Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0003122

Physical Location & Seq

Genomic Coordinates Reverse (-)

38507168 .. 38511209

Gene Structure 4042 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1269400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 615 bp

ATGATGTACCCCACGTCGGTTGGAAACCTCATCAGCTGCATATGTCTAGCCATGGCCATAGCAACCGTAGCCCAACCGGTGCTTGACACCGCCGGACGGGCTCTTCAACCCGGCGTAGACTACTACATCAAACCCGCCATAACCGACAACGGAGGCCGTTTCACTCTGATCAACCGAAACAATTCCTGTCCCTTCTTTGTAGGTCAAGAAAACGTCTCAGGCCCCGAGGGCTTACCCGTGACCTTTGCTCCATTTGCCGCGGGAGCAACTGTGGTGAGGGAGAGCCGTGACCAAATGATCACATTTTCTGCGTCCACAATATGTGTGTCCTCGACTGCATGGAAGGTGGGTGAGACTGATAGAGTCACCCAAAGGCGGTTGATTGTCGCCGGAGCACCTGCATCTGAAGGGCTGCCTCTTAGAAGCTATTTTATGATAAATAGGGTGGAGCCAGAGAGAGCTGGTGTCTACAGTCTGAGGTGGTGTCCCACTGAAGTGTGCCCCACTTGTAGGTTCAGCAACTGTGGGCCCATCGGTGCTTTGGTTGAGAATGGAAAGAGGTTGGTGGCCTTAGATGGTTCTGGGCTTCCTGTTGTGTTTGAGAGGAGGGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0004857 GO:0004866 GO:0004867 GO:0008150 GO:0009892 GO:0010466 GO:0010605 GO:0010951 GO:0015066 GO:0019222 GO:0030162 GO:0030234 GO:0030414 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0043086 GO:0044092 GO:0045861 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0065007 GO:0065009 GO:0080090 GO:0098772

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kunitz_legume

Protein Analysis

205

Amino Acids

21.9

Weight (kDa)

8.46

Isoelectric Point (pI)

48.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kunitz_legume	PF00197	27 - 201	9.4e-42	Trypsin and protease inhibitor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003122)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73260
fragaria_vesca	FvH4_3g17480 FvH4_3g17480 FvH4_3g17790
malus_domestica	MD03G1251400.v1.1 MD11G1269300.v1.1 MD11G1269400.v1.1
prunus_persica	Prupe.4G155200_v2.0.a1
pyrus_communis	pycom03g19880 pycom03g19890
rosa_chinensis	RchiOBHm_Chr5g0029031 RchiOBHm_Chr5g0029081
rosa_laevigata	RLG00000033135 RLG00000033138 RLG00000033181
rosa_multiflora	Rmu_co8286077.1_g000001 Rmu_sc0001608.1_g000003 Rmu_sc0004324.1_g000002 Rmu_sc0010962.1_g000005 Rmu_sc0026501.1_g000002
rosa_roxburghii	Rroxscaffold_1G00050360
rosa_rugosa	Rorug05G0112300 Rorug05G0112400
rosa_samantha	Rh5AG204500 Rh5AG204600 Rh5AG207300 Rh5BG202800 Rh5BG203000 Rh5BG206100 Rh5CG224100 Rh5CG228200 Rh5DG206700 Rh5DG206800 Rh5DG209800
rosa_wichuraiana	Rw5G018540 Rw5G018560 Rw5G018860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	406
Acc36I	ACCTGC	1 cut(s)	406
AccI	GTMKAC	2 cut(s)	117, 468
AccII	CGCG	1 cut(s)	260
AciI	CCGC	5 cut(s)	90, 135, 258, 260, 376
AcoI	YGGCCR	1 cut(s)	54
AcuI	CTGAAG	2 cut(s)	426, 513
AfaI	GTAC	1 cut(s)	8
AfiI	CCNNNNNNNGG	4 cut(s)	16, 96, 375, 510
AgeI	ACCGGT	1 cut(s)	76
AgsI	TTSAA	1 cut(s)	107
AjiI	CACGTC	1 cut(s)	15
AleI	CACNNNNGTG	1 cut(s)	494
AluBI	AGCT	3 cut(s)	36, 426, 461
AluI	AGCT	3 cut(s)	36, 426, 461
Alw21I	GWGCWC	1 cut(s)	397
Alw26I	GTCTC	2 cut(s)	220, 347
AlwNI	CAGNNNCTG	1 cut(s)	522
Ama87I	CYCGRG	1 cut(s)	224
AoxI	GGCC	5 cut(s)	54, 154, 220, 527, 567
ApaI	GGGCCC	1 cut(s)	531
ApeKI	GCWGC	2 cut(s)	36, 412
AsiGI	ACCGGT	1 cut(s)	76
Asp700I	GAANNNNTTC	1 cut(s)	181
AspS9I	GGNCC	3 cut(s)	221, 527, 528
AsuC2I	CCSGG	1 cut(s)	111
AsuHPI	GGTGA	3 cut(s)	286, 358, 362
AvaI	CYCGRG	1 cut(s)	224
BaeGI	GKGCMC	2 cut(s)	503, 531
BalI	TGGCCA	1 cut(s)	56
BanII	GRGCYC	2 cut(s)	103, 531
Bbv12I	GWGCWC	1 cut(s)	397
BbvI	GCAGC	2 cut(s)	23, 399
BccI	CCATC	2 cut(s)	539, 569
BceAI	ACGGC	2 cut(s)	141, 270
BclI	TGATCA	2 cut(s)	168, 297
BcnI	CCSGG	1 cut(s)	111
BcoDI	GTCTC	2 cut(s)	220, 347
BfaI	CTAG	1 cut(s)	47
BfmI	CTRYAG	1 cut(s)	469
BfuAI	ACCTGC	1 cut(s)	406
BglI	GCCNNNNNGGC	2 cut(s)	98, 228
BisI	GCNGC	3 cut(s)	37, 258, 413
BlsI	GCNGC	3 cut(s)	38, 259, 414
Bme1390I	CCNGG	1 cut(s)	111
BmeT110I	CYCGRG	1 cut(s)	224
BmgBI	CACGTC	1 cut(s)	15
BmgT120I	GGNCC	3 cut(s)	221, 527, 528
BmiI	GGNNCC	3 cut(s)	223, 450, 529
BmrFI	CCNGG	1 cut(s)	111
BmsI	GCATC	1 cut(s)	410
BpuMI	CCSGG	1 cut(s)	111
BsaJI	CCNNGG	3 cut(s)	51, 225, 258
BsaWI	WCCGGW	1 cut(s)	76
BsaXI	ACNNNNNCTCC	4 cut(s)	232, 255, 262, 285
Bsc4I	CCNNNNNNNGG	4 cut(s)	16, 96, 375, 510
Bse118I	RCCGGY	1 cut(s)	76
BseDI	CCNNGG	3 cut(s)	51, 225, 258
BseLI	CCNNNNNNNGG	4 cut(s)	16, 96, 375, 510
BseMII	CTCAG	2 cut(s)	231, 467
BseSI	GKGCMC	2 cut(s)	503, 531
BseXI	GCAGC	2 cut(s)	23, 399
Bsh1236I	CGCG	1 cut(s)	260
BshFI	GGCC	5 cut(s)	56, 156, 222, 529, 569
BshTI	ACCGGT	1 cut(s)	76
BsiHKAI	GWGCWC	1 cut(s)	397
BsiHKCI	CYCGRG	1 cut(s)	224
BsiSI	CCGG	4 cut(s)	77, 93, 111, 390
BslFI	GGGAC	2 cut(s)	174, 471
BslI	CCNNNNNNNGG	4 cut(s)	16, 96, 375, 510
BsmAI	GTCTC	2 cut(s)	220, 347
BsmBI	CGTCTC	1 cut(s)	220
BsmFI	GGGAC	2 cut(s)	174, 471
BsnI	GGCC	5 cut(s)	56, 156, 222, 529, 569
BsoBI	CYCGRG	1 cut(s)	224
Bsp120I	GGGCCC	1 cut(s)	527
Bsp1286I	GDGCHC	4 cut(s)	103, 397, 503, 531
Bsp143I	GATC	2 cut(s)	168, 297
Bsp19I	CCATGG	1 cut(s)	51
BspACI	CCGC	5 cut(s)	90, 135, 258, 260, 376
BspANI	GGCC	5 cut(s)	56, 156, 222, 529, 569
BspCNI	CTCAG	2 cut(s)	230, 468
BspFNI	CGCG	1 cut(s)	260
BspLI	GGNNCC	3 cut(s)	223, 450, 529
BspMI	ACCTGC	1 cut(s)	406
BspQI	GCTCTTC	1 cut(s)	108
BsrFI	RCCGGY	1 cut(s)	76
BssAI	RCCGGY	1 cut(s)	76
BssECI	CCNNGG	3 cut(s)	51, 225, 258
BssMI	GATC	2 cut(s)	168, 297
BssT1I	CCWWGG	1 cut(s)	51
Bst4CI	ACNGT	4 cut(s)	67, 271, 473, 524
Bst6I	CTCTTC	1 cut(s)	108
BstDEI	CTNAG	4 cut(s)	217, 419, 476, 571
BstDSI	CCRYGG	2 cut(s)	51, 258
BstFNI	CGCG	1 cut(s)	260
BstKTI	GATC	2 cut(s)	171, 300
BstMAI	GTCTC	2 cut(s)	220, 347
BstMBI	GATC	2 cut(s)	168, 297
BstMWI	GCNNNNNNNGC	5 cut(s)	68, 98, 228, 254, 263
BstSCI	CCNGG	1 cut(s)	109
BstSFI	CTRYAG	1 cut(s)	469
BstSLI	GKGCMC	2 cut(s)	503, 531
BstUI	CGCG	1 cut(s)	260
BstV1I	GCAGC	2 cut(s)	23, 399
BsuRI	GGCC	5 cut(s)	56, 156, 222, 529, 569
BtgI	CCRYGG	2 cut(s)	51, 258
BtrI	CACGTC	1 cut(s)	15
BtsIMutI	CAGTG	1 cut(s)	489
BveI	ACCTGC	1 cut(s)	406
CaiI	CAGNNNCTG	1 cut(s)	522
Cfr10I	RCCGGY	1 cut(s)	76
Cfr13I	GGNCC	3 cut(s)	221, 527, 528
Cfr42I	CCGCGG	1 cut(s)	261
CseI	GACGC	1 cut(s)	300
Csp6I	GTAC	1 cut(s)	7
CspAI	ACCGGT	1 cut(s)	76
CspCI	CAANNNNNGTGG	1 cut(s)	36
CviAII	CATG	2 cut(s)	52, 339
CviQI	GTAC	1 cut(s)	7
DdeI	CTNAG	4 cut(s)	217, 419, 476, 571
DpnI	GATC	2 cut(s)	170, 299
DpnII	GATC	2 cut(s)	168, 297
EaeI	YGGCCR	1 cut(s)	54
Eam1104I	CTCTTC	1 cut(s)	108
EarI	CTCTTC	1 cut(s)	108
Eco130I	CCWWGG	1 cut(s)	51
Eco24I	GRGCYC	2 cut(s)	103, 531
Eco57I	CTGAAG	2 cut(s)	426, 513
Eco88I	CYCGRG	1 cut(s)	224
EcoO109I	RGGNCCY	1 cut(s)	221
EcoT14I	CCWWGG	1 cut(s)	51
EcoT38I	GRGCYC	2 cut(s)	103, 531
ErhI	CCWWGG	1 cut(s)	51
Esp3I	CGTCTC	1 cut(s)	220
FaeI	CATG	2 cut(s)	55, 342
FaiI	YATR	8 cut(s)	41, 43, 53, 59, 140, 322, 340, 434
FaqI	GGGAC	2 cut(s)	174, 471
FatI	CATG	2 cut(s)	51, 338
FauI	CCCGC	2 cut(s)	142, 253
FauNDI	CATATG	1 cut(s)	41
FbaI	TGATCA	2 cut(s)	168, 297
FblI	GTMKAC	2 cut(s)	117, 468
Fnu4HI	GCNGC	3 cut(s)	37, 258, 413
FriOI	GRGCYC	2 cut(s)	103, 531
Fsp4HI	GCNGC	3 cut(s)	37, 258, 413
FspBI	CTAG	1 cut(s)	47
GluI	GCNGC	3 cut(s)	37, 258, 413
HaeIII	GGCC	5 cut(s)	56, 156, 222, 529, 569
HapII	CCGG	4 cut(s)	77, 93, 111, 390
HgaI	GACGC	1 cut(s)	300
Hin1II	CATG	2 cut(s)	55, 342
HinfI	GANTC	1 cut(s)	363
HpaII	CCGG	4 cut(s)	77, 93, 111, 390
HphI	GGTGA	3 cut(s)	286, 358, 362
Hpy166II	GTNNAC	3 cut(s)	118, 315, 469
Hpy188I	TCNGA	3 cut(s)	168, 406, 477
Hpy188III	TCNNGA	1 cut(s)	206
Hpy8I	GTNNAC	3 cut(s)	118, 315, 469
Hpy99I	CGWCG	1 cut(s)	19
HpyAV	CCTTC	3 cut(s)	202, 337, 401
HpyCH4III	ACNGT	4 cut(s)	67, 271, 473, 524
HpyCH4IV	ACGT	2 cut(s)	14, 213
HpyCH4V	TGCA	3 cut(s)	39, 338, 401
HpyF10VI	GCNNNNNNNGC	5 cut(s)	68, 98, 228, 254, 263
HpyF3I	CTNAG	4 cut(s)	217, 419, 476, 571
HpySE526I	ACGT	2 cut(s)	14, 213
Hsp92II	CATG	2 cut(s)	55, 342
Ksp22I	TGATCA	2 cut(s)	168, 297
KspI	CCGCGG	1 cut(s)	261
Kzo9I	GATC	2 cut(s)	168, 297
LguI	GCTCTTC	1 cut(s)	108
LmnI	GCTCC	4 cut(s)	253, 263, 392, 448
Lsp1109I	GCAGC	2 cut(s)	23, 399
LweI	GCATC	1 cut(s)	410
MaeI	CTAG	1 cut(s)	47
MaeII	ACGT	2 cut(s)	14, 213
MaeIII	GTNAC	3 cut(s)	238, 287, 364
MalI	GATC	2 cut(s)	170, 299
MboI	GATC	2 cut(s)	168, 297
MboII	GAAGA	1 cut(s)	95
MhlI	GDGCHC	4 cut(s)	103, 397, 503, 531
MlsI	TGGCCA	1 cut(s)	56
MluCI	AATT	1 cut(s)	181
MluNI	TGGCCA	1 cut(s)	56
MlyI	GAGTC	1 cut(s)	372
Mox20I	TGGCCA	1 cut(s)	56
MroXI	GAANNNNTTC	1 cut(s)	181
MscI	TGGCCA	1 cut(s)	56
MseI	TTAA	1 cut(s)	613
MslI	CAYNNNNRTG	1 cut(s)	494
Msp20I	TGGCCA	1 cut(s)	56
MspA1I	CMGCKG	2 cut(s)	36, 260
MspI	CCGG	4 cut(s)	77, 93, 111, 390
MspR9I	CCNGG	1 cut(s)	111
MvnI	CGCG	1 cut(s)	260
MwoI	GCNNNNNNNGC	5 cut(s)	68, 98, 228, 254, 263
NciI	CCSGG	1 cut(s)	111
NcoI	CCATGG	1 cut(s)	51
NdeI	CATATG	1 cut(s)	41
NdeII	GATC	2 cut(s)	168, 297
NlaIII	CATG	2 cut(s)	55, 342
NlaIV	GGNNCC	3 cut(s)	223, 450, 529
NmuCI	GTSAC	3 cut(s)	238, 287, 364
OliI	CACNNNNGTG	1 cut(s)	494
PaqCI	CACCTGC	1 cut(s)	406
PciSI	GCTCTTC	1 cut(s)	108
PdmI	GAANNNNTTC	1 cut(s)	181
PinAI	ACCGGT	1 cut(s)	76
PkrI	GCNGC	3 cut(s)	38, 259, 414
PleI	GAGTC	1 cut(s)	371
PpsI	GAGTC	1 cut(s)	371
PspN4I	GGNNCC	3 cut(s)	223, 450, 529
PspOMI	GGGCCC	1 cut(s)	527
PspPI	GGNCC	3 cut(s)	221, 527, 528
PstNI	CAGNNNCTG	1 cut(s)	522
PvuII	CAGCTG	1 cut(s)	36
RsaI	GTAC	1 cut(s)	8
RsaNI	GTAC	1 cut(s)	7
RseI	CAYNNNNRTG	1 cut(s)	494
SacII	CCGCGG	1 cut(s)	261
SapI	GCTCTTC	1 cut(s)	108
SaqAI	TTAA	1 cut(s)	613
SatI	GCNGC	3 cut(s)	37, 258, 413
Sau3AI	GATC	2 cut(s)	168, 297
Sau96I	GGNCC	3 cut(s)	221, 527, 528
SchI	GAGTC	1 cut(s)	372
ScrFI	CCNGG	1 cut(s)	111
SduI	GDGCHC	4 cut(s)	103, 397, 503, 531
SfaNI	GCATC	1 cut(s)	410
SfcI	CTRYAG	1 cut(s)	469
Sfr303I	CCGCGG	1 cut(s)	261
SgrBI	CCGCGG	1 cut(s)	261
SmiMI	CAYNNNNRTG	1 cut(s)	494
Sse9I	AATT	1 cut(s)	181
SsiI	CCGC	5 cut(s)	90, 135, 258, 260, 376
SspMI	CTAG	1 cut(s)	47
StyD4I	CCNGG	1 cut(s)	109
StyI	CCWWGG	1 cut(s)	51
TaaI	ACNGT	4 cut(s)	67, 271, 473, 524
TaiI	ACGT	2 cut(s)	17, 216
TaqI	TCGA	1 cut(s)	332
TasI	AATT	1 cut(s)	181
TauI	GCSGC	1 cut(s)	260
Tru1I	TTAA	1 cut(s)	613
Tru9I	TTAA	1 cut(s)	613
TscAI	CASTG	1 cut(s)	496
TseFI	GTSAC	3 cut(s)	238, 287, 364
TseI	GCWGC	2 cut(s)	36, 412
Tsp45I	GTSAC	3 cut(s)	238, 287, 364
TspGWI	ACGGA	1 cut(s)	165
TspRI	CASTG	1 cut(s)	496
XcmI	CCANNNNNNNNNTGG	1 cut(s)	538
XmiI	GTMKAC	2 cut(s)	117, 468
XmnI	GAANNNNTTC	1 cut(s)	181
XspI	CTAG	1 cut(s)	47

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.