Rh5CG228200

alpha-amylase subtilisin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003122
Physical Location & Seq
Forward (+)
23286442 .. 23287107
666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG228200.1

Sequence Viewer

Length: 666 bp
ATGTTATTTTTTCTTTACAGCTTAATATTTTCTTGTCTGAAAATGATGATGTCCCTGAAGAACTTAATTGGAAGCCTGAGCATGATCGGCAGCATATTCATGGCCATGGCAACCATAAACCAAACATCTCCGGTGCTCGATACGTCTGGGAAGGCACTTAAATCCGGCATAGATTACTACATCAAACCCGTCAGTACTTACAAAGGTGGTGCTTTAACTCCAATAAACCGGGAGCCATATTTATGTCCACTCTACGTTGGCCAAGGAAATGTCTCAGGCTGGCCTTTTCAGTTCAAGCCATTTTTTGACGGAGAAACAGTGGTGAGGGAGAGTAGGGATCAAATGATTATATCTCGTGTCTCTACACATTGCAGGCGGTCAAGTTCATGGTCGATTGAAAAAGCTAACCCAGAGGACCAACGGAAATTAATTGCTATGCAAGAAACTGCAAGAGGAAAACTGCCAAATGGTAAATACTTTAGGATTACTAGATCACAGCCGGAGGTGGATGGTAATAGCTACATTCTGAGATGGTGTCCCACTGATCTGTGTTCTAATTGTACATTCGCTGCTTGTGGAAATATTGGTTCTTTAGATTCGAATGGAGGGAAGAGGTTGTTGGCTTTGAATGGTTCTGAGCTTCCGGTTGTGTTTGAAAGGGCATGA

Protein Analysis

221

Amino Acids

24.59

Weight (kDa)

9.2

Isoelectric Point (pI)

48.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 45 - 219 6.7e-33 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 376
AclWI GGATC 1 cut(s) 345
AcoI YGGCCR 2 cut(s) 102, 259
AcuI CTGAAG 1 cut(s) 77
AfaI GTAC 2 cut(s) 196, 562
AgsI TTSAA 4 cut(s) 295, 398, 628, 656
AjuI GAANNNNNNNTTGG 2 cut(s) 602, 634
AluBI AGCT 4 cut(s) 21, 404, 519, 640
AluI AGCT 4 cut(s) 21, 404, 519, 640
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 2 cut(s) 277, 364
AlwI GGATC 1 cut(s) 345
AoxI GGCC 3 cut(s) 102, 259, 281
ApeKI GCWGC 2 cut(s) 90, 569
AseI ATTAAT 1 cut(s) 428
AspS9I GGNCC 1 cut(s) 415
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 1 cut(s) 334
AsuII TTCGAA 1 cut(s) 599
AvaII GGWCC 1 cut(s) 415
BalI TGGCCA 2 cut(s) 104, 261
BauI CACGAG 1 cut(s) 354
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 2 cut(s) 102, 556
BccI CCATC 2 cut(s) 503, 525
BcnI CCSGG 1 cut(s) 230
BcoDI GTCTC 2 cut(s) 277, 364
BfaI CTAG 1 cut(s) 489
BisI GCNGC 2 cut(s) 91, 570
BlsI GCNGC 2 cut(s) 92, 571
BmcAI AGTACT 1 cut(s) 196
Bme1390I CCNGG 1 cut(s) 230
Bme18I GGWCC 1 cut(s) 415
BmgT120I GGNCC 1 cut(s) 415
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 1 cut(s) 230
Bpu10I CCTNAGC 1 cut(s) 77
Bpu14I TTCGAA 1 cut(s) 599
BpuMI CCSGG 1 cut(s) 230
BsaJI CCNNGG 2 cut(s) 105, 262
BsaWI WCCGGW 2 cut(s) 130, 643
BsaXI ACNNNNNCTCC 2 cut(s) 303, 333
Bse3DI GCAATG 1 cut(s) 367
BseDI CCNNGG 2 cut(s) 105, 262
BseGI GGATG 1 cut(s) 514
BseMI GCAATG 1 cut(s) 367
BseMII CTCAG 4 cut(s) 68, 288, 518, 627
BseXI GCAGC 2 cut(s) 102, 556
BshFI GGCC 3 cut(s) 104, 261, 283
BsiHKAI GWGCWC 1 cut(s) 138
BsiSI CCGG 5 cut(s) 131, 165, 229, 500, 644
BslFI GGGAC 2 cut(s) 37, 522
BsmAI GTCTC 2 cut(s) 277, 364
BsmFI GGGAC 2 cut(s) 37, 522
BsnI GGCC 3 cut(s) 104, 261, 283
Bsp119I TTCGAA 1 cut(s) 599
Bsp1286I GDGCHC 1 cut(s) 138
Bsp1407I TGTACA 1 cut(s) 560
Bsp143I GATC 4 cut(s) 84, 337, 491, 544
Bsp19I CCATGG 1 cut(s) 105
BspACI CCGC 1 cut(s) 376
BspANI GGCC 3 cut(s) 104, 261, 283
BspCNI CTCAG 4 cut(s) 69, 287, 519, 628
BspLI GGNNCC 1 cut(s) 234
BspPI GGATC 1 cut(s) 345
BspT104I TTCGAA 1 cut(s) 599
BsrDI GCAATG 1 cut(s) 367
BsrGI TGTACA 1 cut(s) 560
BssECI CCNNGG 2 cut(s) 105, 262
BssMI GATC 4 cut(s) 84, 337, 491, 544
BssSI CACGAG 1 cut(s) 354
BssT1I CCWWGG 2 cut(s) 105, 262
Bst2BI CACGAG 1 cut(s) 354
Bst4CI ACNGT 1 cut(s) 319
Bst6I CTCTTC 1 cut(s) 605
BstAUI TGTACA 1 cut(s) 560
BstBI TTCGAA 1 cut(s) 599
BstC8I GCNNGC 2 cut(s) 281, 374
BstDEI CTNAG 4 cut(s) 77, 274, 527, 636
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 1 cut(s) 514
BstKTI GATC 4 cut(s) 87, 340, 494, 547
BstMAI GTCTC 2 cut(s) 277, 364
BstMBI GATC 4 cut(s) 84, 337, 491, 544
BstMWI GCNNNNNNNGC 1 cut(s) 87
BstSCI CCNGG 1 cut(s) 228
BstV1I GCAGC 2 cut(s) 102, 556
BsuRI GGCC 3 cut(s) 104, 261, 283
BtgI CCRYGG 1 cut(s) 105
BtsCI GGATG 1 cut(s) 514
BtsIMutI CAGTG 2 cut(s) 324, 540
Cac8I GCNNGC 2 cut(s) 281, 374
Cfr13I GGNCC 1 cut(s) 415
Csp6I GTAC 2 cut(s) 195, 561
CviAII CATG 5 cut(s) 82, 100, 106, 387, 663
CviQI GTAC 2 cut(s) 195, 561
DdeI CTNAG 4 cut(s) 77, 274, 527, 636
DpnI GATC 4 cut(s) 86, 339, 493, 546
DpnII GATC 4 cut(s) 84, 337, 491, 544
EaeI YGGCCR 2 cut(s) 102, 259
Eam1104I CTCTTC 1 cut(s) 605
EarI CTCTTC 1 cut(s) 605
Eco130I CCWWGG 2 cut(s) 105, 262
Eco47I GGWCC 1 cut(s) 415
Eco57I CTGAAG 1 cut(s) 77
EcoT14I CCWWGG 2 cut(s) 105, 262
ErhI CCWWGG 2 cut(s) 105, 262
FaeI CATG 5 cut(s) 85, 103, 109, 390, 666
FaqI GGGAC 2 cut(s) 37, 522
FatI CATG 5 cut(s) 81, 99, 105, 386, 662
Fnu4HI GCNGC 2 cut(s) 91, 570
FokI GGATG 1 cut(s) 521
Fsp4HI GCNGC 2 cut(s) 91, 570
FspBI CTAG 1 cut(s) 489
GluI GCNGC 2 cut(s) 91, 570
HaeIII GGCC 3 cut(s) 104, 261, 283
HapII CCGG 5 cut(s) 131, 165, 229, 500, 644
Hin1II CATG 5 cut(s) 85, 103, 109, 390, 666
HinfI GANTC 1 cut(s) 596
HpaII CCGG 5 cut(s) 131, 165, 229, 500, 644
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 1 cut(s) 248
Hpy188I TCNGA 3 cut(s) 39, 528, 637
Hpy8I GTNNAC 1 cut(s) 248
HpyAV CCTTC 1 cut(s) 145
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4IV ACGT 2 cut(s) 143, 255
HpyCH4V TGCA 3 cut(s) 372, 439, 449
HpyF10VI GCNNNNNNNGC 1 cut(s) 87
HpyF3I CTNAG 4 cut(s) 77, 274, 527, 636
HpySE526I ACGT 2 cut(s) 143, 255
Hsp92II CATG 5 cut(s) 85, 103, 109, 390, 666
Kzo9I GATC 4 cut(s) 84, 337, 491, 544
LmnI GCTCC 1 cut(s) 232
Lsp1109I GCAGC 2 cut(s) 102, 556
MaeI CTAG 1 cut(s) 489
MaeII ACGT 2 cut(s) 143, 255
MalI GATC 4 cut(s) 86, 339, 493, 546
MboI GATC 4 cut(s) 84, 337, 491, 544
MboII GAAGA 2 cut(s) 70, 622
MhlI GDGCHC 1 cut(s) 138
MlsI TGGCCA 2 cut(s) 104, 261
MluCI AATT 4 cut(s) 66, 425, 429, 556
MluNI TGGCCA 2 cut(s) 104, 261
MnlI CCTC 6 cut(s) 318, 406, 446, 496, 599, 606
Mox20I TGGCCA 2 cut(s) 104, 261
MscI TGGCCA 2 cut(s) 104, 261
MseI TTAA 5 cut(s) 23, 65, 159, 215, 428
MslI CAYNNNNRTG 3 cut(s) 98, 104, 241
Msp20I TGGCCA 2 cut(s) 104, 261
MspI CCGG 5 cut(s) 131, 165, 229, 500, 644
MspR9I CCNGG 1 cut(s) 230
MwoI GCNNNNNNNGC 1 cut(s) 87
NciI CCSGG 1 cut(s) 230
NcoI CCATGG 1 cut(s) 105
NdeII GATC 4 cut(s) 84, 337, 491, 544
NlaIII CATG 5 cut(s) 85, 103, 109, 390, 666
NlaIV GGNNCC 1 cut(s) 234
NspV TTCGAA 1 cut(s) 599
PfeI GAWTC 1 cut(s) 596
PkrI GCNGC 2 cut(s) 92, 571
PshBI ATTAAT 1 cut(s) 428
PspN4I GGNNCC 1 cut(s) 234
PspPI GGNCC 1 cut(s) 415
RsaI GTAC 2 cut(s) 196, 562
RsaNI GTAC 2 cut(s) 195, 561
RseI CAYNNNNRTG 3 cut(s) 98, 104, 241
SaqAI TTAA 5 cut(s) 23, 65, 159, 215, 428
SatI GCNGC 2 cut(s) 91, 570
Sau3AI GATC 4 cut(s) 84, 337, 491, 544
Sau96I GGNCC 1 cut(s) 415
ScaI AGTACT 1 cut(s) 196
ScrFI CCNGG 1 cut(s) 230
SduI GDGCHC 1 cut(s) 138
SetI ASST 9 cut(s) 23, 146, 208, 258, 406, 507, 521, 617, 642
SfuI TTCGAA 1 cut(s) 599
SinI GGWCC 1 cut(s) 415
SmiMI CAYNNNNRTG 3 cut(s) 98, 104, 241
Sse9I AATT 4 cut(s) 66, 425, 429, 556
SsiI CCGC 1 cut(s) 376
SspI AATATT 2 cut(s) 27, 583
SspMI CTAG 1 cut(s) 489
StyD4I CCNGG 1 cut(s) 228
StyI CCWWGG 2 cut(s) 105, 262
TaaI ACNGT 1 cut(s) 319
TaiI ACGT 2 cut(s) 146, 258
TaqI TCGA 3 cut(s) 138, 392, 599
TasI AATT 4 cut(s) 66, 425, 429, 556
TatI WGTACW 2 cut(s) 194, 560
TfiI GAWTC 1 cut(s) 596
Tru1I TTAA 5 cut(s) 23, 65, 159, 215, 428
Tru9I TTAA 5 cut(s) 23, 65, 159, 215, 428
TscAI CASTG 2 cut(s) 324, 547
TseI GCWGC 2 cut(s) 90, 569
TspDTI ATGAA 2 cut(s) 88, 375
TspGWI ACGGA 2 cut(s) 324, 436
TspRI CASTG 2 cut(s) 324, 547
VpaK11BI GGWCC 1 cut(s) 415
VspI ATTAAT 1 cut(s) 428
XspI CTAG 1 cut(s) 489
ZrmI AGTACT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.