RLG00000033181

alpha-amylase subtilisin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003122
Physical Location & Seq
Forward (+)
24139205 .. 24139828
624 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033181

Sequence Viewer

Length: 624 bp
ATGATGATGTCCCTGAAGAACTTAATTGGAAGCCTGAGCATGATCGGCAGCATATTCATGGCTATGGCAACCATAAACCAAACATCTCCGGTGCTCGATACGTCTGGGAAGGCACTTAAATCCGGCATAGATTACTACATCAAACCCGCCAGTACTTACAAAGGTGGTGCTTTAACTCTAATAAACCGGGAGCCATATTTATGTCCACTCTATGTTGGCCAAGGAAATGTCTCAGGCTGGCCTTTTCAGTTCAAGCCATTTTTCGACGGAGAAACAGTGGTGAAGGAGAGTAGGGATCAAATGATCATATCCCGTGTCTCTACACATTGCAGGCGGTTAAGTTCATGGTCGATTGAAAAAGCTAACCCAGAGAACCAAGGGAAATTAATTGCTATGCAAGAACCTGCAAGAGGTAAACTGCCAAATGGTAAATACTTCAGGATTACTAGATCACAGCCGGAGGTGGATGGTAATAGCTACATTCTAAGATGGTGTCCCACCAATATGTGTCCTAATTGTACATTCGCTGATTGTGGAAATATTGGTTCTTTAGAATCGAATGGAGGGAAGAGGTTCTTGGCTTTGAATGGTTCTGAGCTTCCGGTTGTGTTTGAGAGGGCTTGA

Protein Analysis

208

Amino Acids

22.88

Weight (kDa)

9.17

Isoelectric Point (pI)

41.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 31 - 205 6.7e-32 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 412
AciI CCGC 2 cut(s) 147, 334
AclWI GGATC 1 cut(s) 303
AcoI YGGCCR 1 cut(s) 217
AcuI CTGAAG 2 cut(s) 35, 421
AfaI GTAC 2 cut(s) 154, 520
AfiI CCNNNNNNNGG 1 cut(s) 410
AgsI TTSAA 3 cut(s) 253, 356, 586
AjuI GAANNNNNNNTTGG 2 cut(s) 560, 592
AluBI AGCT 3 cut(s) 362, 477, 598
AluI AGCT 3 cut(s) 362, 477, 598
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 2 cut(s) 235, 322
AlwI GGATC 1 cut(s) 303
AoxI GGCC 2 cut(s) 217, 239
ApeKI GCWGC 1 cut(s) 48
AseI ATTAAT 1 cut(s) 386
Asp700I GAANNNNTTC 1 cut(s) 572
AsuC2I CCSGG 1 cut(s) 188
AsuHPI GGTGA 1 cut(s) 292
BalI TGGCCA 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 1 cut(s) 60
BccI CCATC 2 cut(s) 461, 483
BclI TGATCA 1 cut(s) 303
BcnI CCSGG 1 cut(s) 188
BcoDI GTCTC 2 cut(s) 235, 322
BfaI CTAG 1 cut(s) 447
BfuAI ACCTGC 1 cut(s) 412
BisI GCNGC 1 cut(s) 49
BlsI GCNGC 1 cut(s) 50
BmcAI AGTACT 1 cut(s) 154
Bme1390I CCNGG 1 cut(s) 188
BmiI GGNNCC 1 cut(s) 192
BmrFI CCNGG 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 35
BpuMI CCSGG 1 cut(s) 188
BsaJI CCNNGG 2 cut(s) 220, 376
BsaWI WCCGGW 2 cut(s) 88, 601
BsaXI ACNNNNNCTCC 2 cut(s) 261, 291
Bsc4I CCNNNNNNNGG 1 cut(s) 410
Bse1I ACTGG 1 cut(s) 150
Bse3DI GCAATG 1 cut(s) 325
BseDI CCNNGG 2 cut(s) 220, 376
BseGI GGATG 1 cut(s) 472
BseLI CCNNNNNNNGG 1 cut(s) 410
BseMI GCAATG 1 cut(s) 325
BseMII CTCAG 3 cut(s) 26, 246, 585
BseNI ACTGG 1 cut(s) 150
BseXI GCAGC 1 cut(s) 60
BshFI GGCC 2 cut(s) 219, 241
BsiHKAI GWGCWC 1 cut(s) 96
BsiSI CCGG 5 cut(s) 89, 123, 187, 458, 602
BslFI GGGAC 1 cut(s) 480
BslI CCNNNNNNNGG 1 cut(s) 410
BsmAI GTCTC 2 cut(s) 235, 322
BsmFI GGGAC 1 cut(s) 480
BsnI GGCC 2 cut(s) 219, 241
Bsp1286I GDGCHC 1 cut(s) 96
Bsp1407I TGTACA 1 cut(s) 518
Bsp143I GATC 4 cut(s) 42, 295, 303, 449
BspACI CCGC 2 cut(s) 147, 334
BspANI GGCC 2 cut(s) 219, 241
BspCNI CTCAG 3 cut(s) 27, 245, 586
BspLI GGNNCC 1 cut(s) 192
BspMI ACCTGC 1 cut(s) 412
BspPI GGATC 1 cut(s) 303
BsrDI GCAATG 1 cut(s) 325
BsrGI TGTACA 1 cut(s) 518
BsrI ACTGG 1 cut(s) 150
BssECI CCNNGG 2 cut(s) 220, 376
BssMI GATC 4 cut(s) 42, 295, 303, 449
BssT1I CCWWGG 2 cut(s) 220, 376
Bst4CI ACNGT 1 cut(s) 277
Bst6I CTCTTC 1 cut(s) 563
BstAUI TGTACA 1 cut(s) 518
BstC8I GCNNGC 2 cut(s) 239, 332
BstDEI CTNAG 4 cut(s) 35, 232, 485, 594
BstENI CCTNNNNNAGG 1 cut(s) 408
BstF5I GGATG 1 cut(s) 472
BstKTI GATC 4 cut(s) 45, 298, 306, 452
BstMAI GTCTC 2 cut(s) 235, 322
BstMBI GATC 4 cut(s) 42, 295, 303, 449
BstMWI GCNNNNNNNGC 1 cut(s) 45
BstSCI CCNGG 1 cut(s) 186
BstV1I GCAGC 1 cut(s) 60
BsuRI GGCC 2 cut(s) 219, 241
BtsCI GGATG 1 cut(s) 472
BtsIMutI CAGTG 1 cut(s) 282
BveI ACCTGC 1 cut(s) 412
Cac8I GCNNGC 2 cut(s) 239, 332
Csp6I GTAC 2 cut(s) 153, 519
CviAII CATG 3 cut(s) 40, 58, 345
CviQI GTAC 2 cut(s) 153, 519
DdeI CTNAG 4 cut(s) 35, 232, 485, 594
DpnI GATC 4 cut(s) 44, 297, 305, 451
DpnII GATC 4 cut(s) 42, 295, 303, 449
EaeI YGGCCR 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 563
EarI CTCTTC 1 cut(s) 563
Eco130I CCWWGG 2 cut(s) 220, 376
Eco57I CTGAAG 2 cut(s) 35, 421
EcoNI CCTNNNNNAGG 1 cut(s) 408
EcoT14I CCWWGG 2 cut(s) 220, 376
ErhI CCWWGG 2 cut(s) 220, 376
FaeI CATG 3 cut(s) 43, 61, 348
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 1 cut(s) 480
FatI CATG 3 cut(s) 39, 57, 344
FauI CCCGC 1 cut(s) 154
FbaI TGATCA 1 cut(s) 303
Fnu4HI GCNGC 1 cut(s) 49
FokI GGATG 1 cut(s) 479
Fsp4HI GCNGC 1 cut(s) 49
FspBI CTAG 1 cut(s) 447
GluI GCNGC 1 cut(s) 49
HaeIII GGCC 2 cut(s) 219, 241
HapII CCGG 5 cut(s) 89, 123, 187, 458, 602
Hin1II CATG 3 cut(s) 43, 61, 348
HinfI GANTC 1 cut(s) 554
HpaII CCGG 5 cut(s) 89, 123, 187, 458, 602
HphI GGTGA 1 cut(s) 292
Hpy166II GTNNAC 2 cut(s) 206, 416
Hpy188I TCNGA 1 cut(s) 595
Hpy188III TCNNGA 1 cut(s) 439
Hpy8I GTNNAC 2 cut(s) 206, 416
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 2 cut(s) 103, 277
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 3 cut(s) 330, 397, 407
HpyF10VI GCNNNNNNNGC 1 cut(s) 45
HpyF3I CTNAG 4 cut(s) 35, 232, 485, 594
HpySE526I ACGT 1 cut(s) 101
Hsp92II CATG 3 cut(s) 43, 61, 348
Ksp22I TGATCA 1 cut(s) 303
Kzo9I GATC 4 cut(s) 42, 295, 303, 449
LmnI GCTCC 1 cut(s) 190
Lsp1109I GCAGC 1 cut(s) 60
MaeI CTAG 1 cut(s) 447
MaeII ACGT 1 cut(s) 101
MalI GATC 4 cut(s) 44, 297, 305, 451
MboI GATC 4 cut(s) 42, 295, 303, 449
MboII GAAGA 2 cut(s) 28, 580
MhlI GDGCHC 1 cut(s) 96
MlsI TGGCCA 1 cut(s) 219
MluCI AATT 4 cut(s) 24, 383, 387, 514
MluNI TGGCCA 1 cut(s) 219
MnlI CCTC 5 cut(s) 404, 454, 557, 564, 609
Mox20I TGGCCA 1 cut(s) 219
MroXI GAANNNNTTC 1 cut(s) 572
MscI TGGCCA 1 cut(s) 219
MseI TTAA 5 cut(s) 23, 117, 173, 338, 386
MslI CAYNNNNRTG 4 cut(s) 56, 62, 199, 503
Msp20I TGGCCA 1 cut(s) 219
MspI CCGG 5 cut(s) 89, 123, 187, 458, 602
MspR9I CCNGG 1 cut(s) 188
MwoI GCNNNNNNNGC 1 cut(s) 45
NciI CCSGG 1 cut(s) 188
NdeII GATC 4 cut(s) 42, 295, 303, 449
NlaIII CATG 3 cut(s) 43, 61, 348
NlaIV GGNNCC 1 cut(s) 192
PdmI GAANNNNTTC 1 cut(s) 572
PfeI GAWTC 1 cut(s) 554
PkrI GCNGC 1 cut(s) 50
PshBI ATTAAT 1 cut(s) 386
PspN4I GGNNCC 1 cut(s) 192
RsaI GTAC 2 cut(s) 154, 520
RsaNI GTAC 2 cut(s) 153, 519
RseI CAYNNNNRTG 4 cut(s) 56, 62, 199, 503
SaqAI TTAA 5 cut(s) 23, 117, 173, 338, 386
SatI GCNGC 1 cut(s) 49
Sau3AI GATC 4 cut(s) 42, 295, 303, 449
ScaI AGTACT 1 cut(s) 154
ScrFI CCNGG 1 cut(s) 188
SduI GDGCHC 1 cut(s) 96
SetI ASST 9 cut(s) 104, 166, 364, 406, 415, 465, 479, 575, 600
SmiMI CAYNNNNRTG 4 cut(s) 56, 62, 199, 503
Sse9I AATT 4 cut(s) 24, 383, 387, 514
SsiI CCGC 2 cut(s) 147, 334
SspI AATATT 1 cut(s) 541
SspMI CTAG 1 cut(s) 447
StyD4I CCNGG 1 cut(s) 186
StyI CCWWGG 2 cut(s) 220, 376
TaaI ACNGT 1 cut(s) 277
TaiI ACGT 1 cut(s) 104
TaqI TCGA 4 cut(s) 96, 264, 350, 557
TasI AATT 4 cut(s) 24, 383, 387, 514
TatI WGTACW 2 cut(s) 152, 518
TfiI GAWTC 1 cut(s) 554
Tru1I TTAA 5 cut(s) 23, 117, 173, 338, 386
Tru9I TTAA 5 cut(s) 23, 117, 173, 338, 386
TscAI CASTG 1 cut(s) 282
TseI GCWGC 1 cut(s) 48
TspDTI ATGAA 2 cut(s) 46, 333
TspGWI ACGGA 1 cut(s) 282
TspRI CASTG 1 cut(s) 282
VspI ATTAAT 1 cut(s) 386
XagI CCTNNNNNAGG 1 cut(s) 408
XmnI GAANNNNTTC 1 cut(s) 572
XspI CTAG 1 cut(s) 447
ZrmI AGTACT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.