pycom03g19880

alpha-amylase subtilisin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003122
Physical Location & Seq
Forward (+)
20508789 .. 20509557
769 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g19880.1

Sequence Viewer

Length: 720 bp
ATGTCTATGAAGTTGATTGGGAGCCTCTGCAGCTGCATATGGCTTGTCATGGCCGTATCAACCCTAGCCCAAACATCCGATGACTCGAATGCTGTGCTTGACACTGCCGGGCAGGCTCTTCAATCCGGTGTGGACTACTACATCAATCCCGCTATTACCGACAGTGGCGGGCGTTTCACTTTGATCAACAGAACTGGATGTCCACTAAATGTTGGTCAAGAAAATGTCTCAGGTCCTGAGGGCTTGCCTGTGACATTTTCTCCTTTTGTTGAGGGGGAAACTGTGGTGAGGGAAGGAAGGGACCAAAAGATCACATTCTCAGCATCCACAATCTGTGTGCAATCAACTGCATGGAAGAAGACGAAAACCAAGGCCTTCCTGGCCCTGCCGGAAACTACTTCCGGATTACAAAGCAGGCAACACCAGATGGTGTGTACAATCTGGAATGGTGCCCTACTGAAGTGTGTCCAACTTGTAGGTTCATTTGTGGATCTGTTGGTGCTATTGTTGAGAATGACAAGAGGCTGCTGGCCTTGGATGGTTCAGCGCTTCCTGTTGTGTTTGAGAGGGCTTAAATTTAAATATGTCCACATATTATATGGGAAACTAATTAAGCGGACCTTAATTATAGTGAGTTATTTCTGTAATAATGGTAGTGATCATGTCCTAGCTGACTATGATCATCGATCTCTTCTCTCTTTTACTGTATCTGAATTATAA

Protein Analysis

240

Amino Acids

26.43

Weight (kDa)

8.73

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 32 - 124 1.4e-24 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 718
AccB1I GGYRCC 1 cut(s) 449
AccIII TCCGGA 1 cut(s) 401
AciI CCGC 3 cut(s) 150, 168, 616
AclWI GGATC 1 cut(s) 498
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 1 cut(s) 575
AcuI CTGAAG 1 cut(s) 479
AfaI GTAC 1 cut(s) 436
AfeI AGCGCT 1 cut(s) 548
AgsI TTSAA 1 cut(s) 122
AjnI CCWGG 1 cut(s) 378
AloI GAACNNNNNNTCC 2 cut(s) 184, 216
AluBI AGCT 2 cut(s) 33, 671
AluI AGCT 2 cut(s) 33, 671
Alw26I GTCTC 1 cut(s) 232
AlwI GGATC 1 cut(s) 498
AlwNI CAGNNNCTG 1 cut(s) 236
Aor13HI TCCGGA 1 cut(s) 401
Aor51HI AGCGCT 1 cut(s) 548
AoxI GGCC 4 cut(s) 51, 372, 381, 530
ApeKI GCWGC 3 cut(s) 30, 33, 525
ApoI RAATTY 1 cut(s) 575
AspLEI GCGC 1 cut(s) 549
AspS9I GGNCC 4 cut(s) 233, 301, 382, 618
AsuC2I CCSGG 1 cut(s) 109
AsuHPI GGTGA 1 cut(s) 298
AvaII GGWCC 3 cut(s) 233, 301, 618
AxyI CCTNAGG 1 cut(s) 237
BaeGI GKGCMC 1 cut(s) 454
BanI GGYRCC 1 cut(s) 449
BbsI GAAGAC 1 cut(s) 365
BbvI GCAGC 3 cut(s) 20, 42, 512
BccI CCATC 2 cut(s) 421, 532
BceAI ACGGC 1 cut(s) 38
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BciT130I CCWGG 1 cut(s) 380
BclI TGATCA 3 cut(s) 183, 658, 679
BcnI CCSGG 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 232
BfaI CTAG 2 cut(s) 65, 668
BfmI CTRYAG 1 cut(s) 28
BfoI RGCGCY 1 cut(s) 550
BglI GCCNNNNNGGC 2 cut(s) 113, 380
BisI GCNGC 3 cut(s) 31, 34, 526
BlsI GCNGC 3 cut(s) 32, 35, 527
Bme1390I CCNGG 2 cut(s) 109, 380
Bme18I GGWCC 3 cut(s) 233, 301, 618
BmgT120I GGNCC 4 cut(s) 233, 301, 382, 618
BmiI GGNNCC 3 cut(s) 23, 302, 451
BmrFI CCNGG 2 cut(s) 109, 380
BmsI GCATC 1 cut(s) 332
BpiI GAAGAC 1 cut(s) 365
BpuMI CCSGG 1 cut(s) 109
Bsa29I ATCGAT 1 cut(s) 685
BsaJI CCNNGG 2 cut(s) 369, 533
BsaWI WCCGGW 2 cut(s) 125, 401
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bse1I ACTGG 1 cut(s) 199
Bse21I CCTNAGG 1 cut(s) 237
BseAI TCCGGA 1 cut(s) 401
BseBI CCWGG 1 cut(s) 380
BseCI ATCGAT 1 cut(s) 685
BseDI CCNNGG 2 cut(s) 369, 533
BseGI GGATG 4 cut(s) 74, 203, 323, 543
BseMII CTCAG 3 cut(s) 228, 243, 333
BseNI ACTGG 1 cut(s) 199
BseSI GKGCMC 1 cut(s) 454
BseXI GCAGC 3 cut(s) 20, 42, 512
BshFI GGCC 4 cut(s) 53, 374, 383, 532
BshNI GGYRCC 1 cut(s) 449
BshVI ATCGAT 1 cut(s) 685
BsiSI CCGG 4 cut(s) 108, 126, 389, 402
BslFI GGGAC 1 cut(s) 314
BsmAI GTCTC 1 cut(s) 232
BsmFI GGGAC 1 cut(s) 314
BsmI GAATGC 1 cut(s) 94
BsnI GGCC 4 cut(s) 53, 374, 383, 532
Bsp1286I GDGCHC 1 cut(s) 454
Bsp13I TCCGGA 1 cut(s) 401
Bsp1407I TGTACA 1 cut(s) 434
Bsp143I GATC 6 cut(s) 183, 309, 490, 658, 679, 686
BspACI CCGC 3 cut(s) 150, 168, 616
BspANI GGCC 4 cut(s) 53, 374, 383, 532
BspCNI CTCAG 3 cut(s) 229, 242, 332
BspDI ATCGAT 1 cut(s) 685
BspEI TCCGGA 1 cut(s) 401
BspLI GGNNCC 3 cut(s) 23, 302, 451
BspMAI CTGCAG 1 cut(s) 32
BspPI GGATC 1 cut(s) 498
BspQI GCTCTTC 1 cut(s) 123
BspT107I GGYRCC 1 cut(s) 449
BsrGI TGTACA 1 cut(s) 434
BsrI ACTGG 1 cut(s) 199
BssECI CCNNGG 2 cut(s) 369, 533
BssMI GATC 6 cut(s) 183, 309, 490, 658, 679, 686
BssT1I CCWWGG 2 cut(s) 369, 533
Bst2UI CCWGG 1 cut(s) 380
Bst4CI ACNGT 3 cut(s) 164, 283, 706
Bst6I CTCTTC 2 cut(s) 123, 696
BstAUI TGTACA 1 cut(s) 434
BstC8I GCNNGC 5 cut(s) 114, 170, 245, 416, 530
BstDEI CTNAG 3 cut(s) 229, 237, 319
BstF5I GGATG 4 cut(s) 74, 203, 323, 543
BstH2I RGCGCY 1 cut(s) 550
BstHHI GCGC 1 cut(s) 549
BstKTI GATC 6 cut(s) 186, 312, 493, 661, 682, 689
BstMAI GTCTC 1 cut(s) 232
BstMBI GATC 6 cut(s) 183, 309, 490, 658, 679, 686
BstMWI GCNNNNNNNGC 3 cut(s) 30, 113, 380
BstNI CCWGG 1 cut(s) 380
BstSCI CCNGG 2 cut(s) 107, 378
BstSFI CTRYAG 1 cut(s) 28
BstSLI GKGCMC 1 cut(s) 454
BstV1I GCAGC 3 cut(s) 20, 42, 512
BstV2I GAAGAC 1 cut(s) 365
BstX2I RGATCY 1 cut(s) 490
BstYI RGATCY 1 cut(s) 490
Bsu15I ATCGAT 1 cut(s) 685
Bsu36I CCTNAGG 1 cut(s) 237
BsuRI GGCC 4 cut(s) 53, 374, 383, 532
BsuTUI ATCGAT 1 cut(s) 685
BtsCI GGATG 4 cut(s) 74, 203, 323, 543
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 2 cut(s) 102, 169
Cac8I GCNNGC 5 cut(s) 114, 170, 245, 416, 530
CaiI CAGNNNCTG 1 cut(s) 236
CfoI GCGC 1 cut(s) 549
Cfr13I GGNCC 4 cut(s) 233, 301, 382, 618
ClaI ATCGAT 1 cut(s) 685
Csp6I GTAC 1 cut(s) 435
CviAII CATG 3 cut(s) 49, 351, 662
CviQI GTAC 1 cut(s) 435
DdeI CTNAG 3 cut(s) 229, 237, 319
DpnI GATC 6 cut(s) 185, 311, 492, 660, 681, 688
DpnII GATC 6 cut(s) 183, 309, 490, 658, 679, 686
DraI TTTAAA 1 cut(s) 580
EaeI YGGCCR 1 cut(s) 51
Eam1104I CTCTTC 2 cut(s) 123, 696
EarI CTCTTC 2 cut(s) 123, 696
Eco130I CCWWGG 2 cut(s) 369, 533
Eco147I AGGCCT 1 cut(s) 374
Eco47I GGWCC 3 cut(s) 233, 301, 618
Eco47III AGCGCT 1 cut(s) 548
Eco57I CTGAAG 1 cut(s) 479
Eco81I CCTNAGG 1 cut(s) 237
EcoO109I RGGNCCY 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 378
EcoT14I CCWWGG 2 cut(s) 369, 533
ErhI CCWWGG 2 cut(s) 369, 533
FaeI CATG 3 cut(s) 52, 354, 665
FalI AAGNNNNNCTT 2 cut(s) 605, 637
FaqI GGGAC 1 cut(s) 314
FatI CATG 3 cut(s) 48, 350, 661
FauI CCCGC 2 cut(s) 157, 161
FauNDI CATATG 1 cut(s) 38
FbaI TGATCA 3 cut(s) 183, 658, 679
Fnu4HI GCNGC 3 cut(s) 31, 34, 526
FokI GGATG 4 cut(s) 61, 210, 310, 550
Fsp4HI GCNGC 3 cut(s) 31, 34, 526
FspBI CTAG 2 cut(s) 65, 668
GlaI GCGC 1 cut(s) 548
GluI GCNGC 3 cut(s) 31, 34, 526
HaeII RGCGCY 1 cut(s) 550
HaeIII GGCC 4 cut(s) 53, 374, 383, 532
HapII CCGG 4 cut(s) 108, 126, 389, 402
HhaI GCGC 1 cut(s) 549
Hin1II CATG 3 cut(s) 52, 354, 665
Hin6I GCGC 1 cut(s) 547
HinP1I GCGC 1 cut(s) 547
HinfI GANTC 1 cut(s) 83
HpaII CCGG 4 cut(s) 108, 126, 389, 402
HphI GGTGA 1 cut(s) 298
Hpy166II GTNNAC 4 cut(s) 133, 203, 435, 589
Hpy188I TCNGA 2 cut(s) 79, 712
Hpy188III TCNNGA 4 cut(s) 218, 236, 402, 442
Hpy8I GTNNAC 4 cut(s) 133, 203, 435, 589
HpyAV CCTTC 3 cut(s) 287, 291, 385
HpyCH4III ACNGT 3 cut(s) 164, 283, 706
HpyCH4V TGCA 4 cut(s) 30, 36, 340, 350
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 113, 380
HpyF3I CTNAG 3 cut(s) 229, 237, 319
Hsp92II CATG 3 cut(s) 52, 354, 665
HspAI GCGC 1 cut(s) 547
Kpn2I TCCGGA 1 cut(s) 401
Ksp22I TGATCA 3 cut(s) 183, 658, 679
Kzo9I GATC 6 cut(s) 183, 309, 490, 658, 679, 686
LguI GCTCTTC 1 cut(s) 123
LmnI GCTCC 1 cut(s) 21
Lsp1109I GCAGC 3 cut(s) 20, 42, 512
LweI GCATC 1 cut(s) 332
MaeI CTAG 2 cut(s) 65, 668
MaeIII GTNAC 1 cut(s) 250
MalI GATC 6 cut(s) 185, 311, 492, 660, 681, 688
MboI GATC 6 cut(s) 183, 309, 490, 658, 679, 686
MboII GAAGA 4 cut(s) 110, 367, 370, 683
MflI RGATCY 1 cut(s) 490
MhlI GDGCHC 1 cut(s) 454
MluCI AATT 4 cut(s) 575, 609, 624, 713
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 493
MnlI CCTC 6 cut(s) 35, 232, 265, 282, 515, 560
MroI TCCGGA 1 cut(s) 401
MseI TTAA 4 cut(s) 573, 579, 612, 623
MspA1I CMGCKG 1 cut(s) 33
MspI CCGG 4 cut(s) 108, 126, 389, 402
MspR9I CCNGG 2 cut(s) 109, 380
Mva1269I GAATGC 1 cut(s) 94
MvaI CCWGG 1 cut(s) 380
MwoI GCNNNNNNNGC 3 cut(s) 30, 113, 380
NciI CCSGG 1 cut(s) 109
NdeI CATATG 1 cut(s) 38
NdeII GATC 6 cut(s) 183, 309, 490, 658, 679, 686
NlaIII CATG 3 cut(s) 52, 354, 665
NlaIV GGNNCC 3 cut(s) 23, 302, 451
NmuCI GTSAC 1 cut(s) 250
PceI AGGCCT 1 cut(s) 374
PciSI GCTCTTC 1 cut(s) 123
PctI GAATGC 1 cut(s) 94
PkrI GCNGC 3 cut(s) 32, 35, 527
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
PpuMI RGGWCCY 1 cut(s) 233
PsiI TTATAA 1 cut(s) 718
Psp5II RGGWCCY 1 cut(s) 233
Psp6I CCWGG 1 cut(s) 378
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 3 cut(s) 23, 302, 451
PspPI GGNCC 4 cut(s) 233, 301, 382, 618
PspPPI RGGWCCY 1 cut(s) 233
PstI CTGCAG 1 cut(s) 32
PstNI CAGNNNCTG 1 cut(s) 236
PsuI RGATCY 1 cut(s) 490
PvuII CAGCTG 1 cut(s) 33
RsaI GTAC 1 cut(s) 436
RsaNI GTAC 1 cut(s) 435
SapI GCTCTTC 1 cut(s) 123
SaqAI TTAA 4 cut(s) 573, 579, 612, 623
SatI GCNGC 3 cut(s) 31, 34, 526
Sau3AI GATC 6 cut(s) 183, 309, 490, 658, 679, 686
Sau96I GGNCC 4 cut(s) 233, 301, 382, 618
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 109, 380
SduI GDGCHC 1 cut(s) 454
SetI ASST 5 cut(s) 35, 235, 481, 623, 673
SfaNI GCATC 1 cut(s) 332
SfcI CTRYAG 1 cut(s) 28
SfiI GGCCNNNNNGGCC 1 cut(s) 380
SinI GGWCC 3 cut(s) 233, 301, 618
SmiI ATTTAAAT 1 cut(s) 580
Sse9I AATT 4 cut(s) 575, 609, 624, 713
SseBI AGGCCT 1 cut(s) 374
SsiI CCGC 3 cut(s) 150, 168, 616
SspMI CTAG 2 cut(s) 65, 668
StuI AGGCCT 1 cut(s) 374
StyD4I CCNGG 2 cut(s) 107, 378
StyI CCWWGG 2 cut(s) 369, 533
SwaI ATTTAAAT 1 cut(s) 580
TaaI ACNGT 3 cut(s) 164, 283, 706
TaqI TCGA 2 cut(s) 86, 685
TasI AATT 4 cut(s) 575, 609, 624, 713
TatI WGTACW 1 cut(s) 434
Tru1I TTAA 4 cut(s) 573, 579, 612, 623
Tru9I TTAA 4 cut(s) 573, 579, 612, 623
TscAI CASTG 2 cut(s) 109, 169
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 3 cut(s) 30, 33, 525
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 2 cut(s) 23, 471
TspRI CASTG 2 cut(s) 109, 169
VpaK11BI GGWCC 3 cut(s) 233, 301, 618
XapI RAATTY 1 cut(s) 575
XcmI CCANNNNNNNNNTGG 2 cut(s) 376, 596
XspI CTAG 2 cut(s) 65, 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.