Rh5DG206800

alpha-amylase subtilisin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003122
Physical Location & Seq
Reverse (-)
22843450 .. 22844329
880 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG206800.1

Sequence Viewer

Length: 603 bp
ATGATGTCCATGAAGTTGATCGGAAGCATATGGCTAGTAATGGCCATGGCAACCGTAGCCCAAGATAGCGCTCCAGTGCTCGACACCTCAGGGCAGGCTCTTCAATCCGGTGTAGACTACTACATCAAACCCGCCATCACCGACAACGGCGGCCGTTTCACTTTGATCAACCGAAACGACTCCTGTCCTTTGTATGTTGGTCAAGAAAACACCTCAGGCCCCAAGGGCTACCCGGTGACCTTTGCGCCTTTCGCAGAGGGTGAGACGGTGGTAAGAGTGGGTAGGGATCAGAAGATCACATTCTCTGCATCCACAATCTGTGTGCAATCGACTGCATGGAAGGTGGGTGAGACTCAGTCCGAGACCCAAAGGAGATTGATTGTCACCGGAATTGATGAAAACGAAAGCCGTCCTACCGGAAACTACTTCAAGATTAACAAACAGGCAGATCTTGATGGCATTTACAATTTCGAATGGTGCCCTACTGAAGTGTGCCCGATTTGTAAGTTCATCTGCTCAGATGTGGGTGCTTTGGTTGAGAATGACAAGAGGTTGTTGGCCTTGGATGGTTCTGTGCTTCCTGTTGTCTTTGAGAGGGCCTAA

Protein Analysis

200

Amino Acids

21.89

Weight (kDa)

4.83

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 26 - 198 2.6e-49 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 477
AccI GTMKAC 1 cut(s) 114
AciI CCGC 2 cut(s) 132, 150
AclWI GGATC 1 cut(s) 294
AcoI YGGCCR 2 cut(s) 42, 151
AcuI CTGAAG 1 cut(s) 507
AfeI AGCGCT 1 cut(s) 70
AgsI TTSAA 2 cut(s) 104, 430
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 3 cut(s) 257, 344, 356
AlwI GGATC 1 cut(s) 294
Aor51HI AGCGCT 1 cut(s) 70
AoxI GGCC 5 cut(s) 42, 151, 217, 558, 597
AspLEI GCGC 2 cut(s) 71, 247
AspS9I GGNCC 2 cut(s) 218, 597
AsuC2I CCSGG 1 cut(s) 233
AsuHPI GGTGA 5 cut(s) 130, 247, 272, 359, 376
AsuII TTCGAA 1 cut(s) 471
AxyI CCTNAGG 2 cut(s) 88, 214
BaeGI GKGCMC 2 cut(s) 482, 497
BalI TGGCCA 1 cut(s) 44
BanI GGYRCC 1 cut(s) 477
Bbv12I GWGCWC 1 cut(s) 81
BccI CCATC 3 cut(s) 143, 449, 560
BceAI ACGGC 3 cut(s) 138, 163, 393
BclI TGATCA 1 cut(s) 165
BcnI CCSGG 1 cut(s) 233
BcoDI GTCTC 3 cut(s) 257, 344, 356
BfaI CTAG 1 cut(s) 35
BfoI RGCGCY 1 cut(s) 72
BglI GCCNNNNNGGC 1 cut(s) 225
BglII AGATCT 1 cut(s) 448
BisI GCNGC 1 cut(s) 151
BlsI GCNGC 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 233
BmgT120I GGNCC 2 cut(s) 218, 597
BmiI GGNNCC 2 cut(s) 220, 479
BmrFI CCNGG 1 cut(s) 233
BmsI GCATC 1 cut(s) 317
BoxI GACNNNNGTC 1 cut(s) 183
BpmI CTGGAG 1 cut(s) 57
Bpu14I TTCGAA 1 cut(s) 471
BpuMI CCSGG 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 356
BsaJI CCNNGG 3 cut(s) 45, 222, 561
BsaWI WCCGGW 3 cut(s) 107, 386, 416
Bse1I ACTGG 1 cut(s) 74
Bse21I CCTNAGG 2 cut(s) 88, 214
BseDI CCNNGG 3 cut(s) 45, 222, 561
BseGI GGATG 2 cut(s) 308, 571
BseMII CTCAG 4 cut(s) 102, 228, 368, 531
BseNI ACTGG 1 cut(s) 74
BseSI GKGCMC 2 cut(s) 482, 497
BseX3I CGGCCG 1 cut(s) 151
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 5 cut(s) 44, 153, 219, 560, 599
BshNI GGYRCC 1 cut(s) 477
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 81
BsiSI CCGG 4 cut(s) 108, 233, 387, 417
BsmAI GTCTC 3 cut(s) 257, 344, 356
BsmBI CGTCTC 1 cut(s) 257
BsnI GGCC 5 cut(s) 44, 153, 219, 560, 599
Bso31I GGTCTC 1 cut(s) 356
Bsp119I TTCGAA 1 cut(s) 471
Bsp1286I GDGCHC 3 cut(s) 81, 482, 497
Bsp143I GATC 5 cut(s) 18, 165, 286, 294, 448
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 2 cut(s) 132, 150
BspANI GGCC 5 cut(s) 44, 153, 219, 560, 599
BspCNI CTCAG 4 cut(s) 101, 227, 367, 530
BspLI GGNNCC 2 cut(s) 220, 479
BspPI GGATC 1 cut(s) 294
BspQI GCTCTTC 1 cut(s) 105
BspT104I TTCGAA 1 cut(s) 471
BspT107I GGYRCC 1 cut(s) 477
BspTNI GGTCTC 1 cut(s) 356
BsrI ACTGG 1 cut(s) 74
BssECI CCNNGG 3 cut(s) 45, 222, 561
BssMI GATC 5 cut(s) 18, 165, 286, 294, 448
BssT1I CCWWGG 3 cut(s) 45, 222, 561
Bst4CI ACNGT 2 cut(s) 55, 268
Bst6I CTCTTC 1 cut(s) 105
BstBI TTCGAA 1 cut(s) 471
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 4 cut(s) 88, 214, 354, 517
BstDSI CCRYGG 1 cut(s) 45
BstEII GGTNACC 1 cut(s) 235
BstF5I GGATG 2 cut(s) 308, 571
BstH2I RGCGCY 1 cut(s) 72
BstHHI GCGC 2 cut(s) 71, 247
BstKTI GATC 5 cut(s) 21, 168, 289, 297, 451
BstMAI GTCTC 3 cut(s) 257, 344, 356
BstMBI GATC 5 cut(s) 18, 165, 286, 294, 448
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 3 cut(s) 56, 225, 251
BstPAI GACNNNNGTC 1 cut(s) 183
BstPI GGTNACC 1 cut(s) 235
BstSCI CCNGG 1 cut(s) 231
BstSLI GKGCMC 2 cut(s) 482, 497
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BstZI CGGCCG 1 cut(s) 151
Bsu36I CCTNAGG 2 cut(s) 88, 214
BsuRI GGCC 5 cut(s) 44, 153, 219, 560, 599
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 2 cut(s) 308, 571
BtsIMutI CAGTG 1 cut(s) 81
Cac8I GCNNGC 1 cut(s) 96
CfoI GCGC 2 cut(s) 71, 247
Cfr13I GGNCC 2 cut(s) 218, 597
CviAII CATG 3 cut(s) 10, 46, 336
DdeI CTNAG 4 cut(s) 88, 214, 354, 517
DpnI GATC 5 cut(s) 20, 167, 288, 296, 450
DpnII GATC 5 cut(s) 18, 165, 286, 294, 448
EaeI YGGCCR 2 cut(s) 42, 151
EagI CGGCCG 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 105
EarI CTCTTC 1 cut(s) 105
EclXI CGGCCG 1 cut(s) 151
Eco130I CCWWGG 3 cut(s) 45, 222, 561
Eco31I GGTCTC 1 cut(s) 356
Eco47III AGCGCT 1 cut(s) 70
Eco52I CGGCCG 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 507
Eco81I CCTNAGG 2 cut(s) 88, 214
Eco91I GGTNACC 1 cut(s) 235
EcoO109I RGGNCCY 2 cut(s) 218, 597
EcoO65I GGTNACC 1 cut(s) 235
EcoT14I CCWWGG 3 cut(s) 45, 222, 561
ErhI CCWWGG 3 cut(s) 45, 222, 561
Esp3I CGTCTC 1 cut(s) 257
FaeI CATG 3 cut(s) 13, 49, 339
FaiI YATR 6 cut(s) 11, 29, 31, 47, 195, 337
FatI CATG 3 cut(s) 9, 45, 335
FauI CCCGC 1 cut(s) 139
FauNDI CATATG 1 cut(s) 29
FbaI TGATCA 1 cut(s) 165
FblI GTMKAC 1 cut(s) 114
Fnu4HI GCNGC 1 cut(s) 151
FokI GGATG 2 cut(s) 295, 578
Fsp4HI GCNGC 1 cut(s) 151
FspBI CTAG 1 cut(s) 35
GlaI GCGC 2 cut(s) 70, 246
GluI GCNGC 1 cut(s) 151
GsuI CTGGAG 1 cut(s) 57
HaeII RGCGCY 1 cut(s) 72
HaeIII GGCC 5 cut(s) 44, 153, 219, 560, 599
HapII CCGG 4 cut(s) 108, 233, 387, 417
HhaI GCGC 2 cut(s) 71, 247
Hin1II CATG 3 cut(s) 13, 49, 339
Hin6I GCGC 2 cut(s) 69, 245
HinP1I GCGC 2 cut(s) 69, 245
HinfI GANTC 2 cut(s) 179, 352
HpaII CCGG 4 cut(s) 108, 233, 387, 417
HphI GGTGA 5 cut(s) 130, 247, 272, 359, 376
Hpy166II GTNNAC 1 cut(s) 115
Hpy188I TCNGA 4 cut(s) 23, 291, 361, 520
Hpy188III TCNNGA 3 cut(s) 203, 430, 452
Hpy8I GTNNAC 1 cut(s) 115
HpyAV CCTTC 1 cut(s) 334
HpyCH4III ACNGT 2 cut(s) 55, 268
HpyCH4V TGCA 3 cut(s) 308, 325, 335
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 225, 251
HpyF3I CTNAG 4 cut(s) 88, 214, 354, 517
Hsp92II CATG 3 cut(s) 13, 49, 339
HspAI GCGC 2 cut(s) 69, 245
Ksp22I TGATCA 1 cut(s) 165
Kzo9I GATC 5 cut(s) 18, 165, 286, 294, 448
LguI GCTCTTC 1 cut(s) 105
LmnI GCTCC 1 cut(s) 76
LweI GCATC 1 cut(s) 317
MaeI CTAG 1 cut(s) 35
MaeIII GTNAC 2 cut(s) 235, 382
MalI GATC 5 cut(s) 20, 167, 288, 296, 450
MboI GATC 5 cut(s) 18, 165, 286, 294, 448
MboII GAAGA 2 cut(s) 92, 304
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 3 cut(s) 81, 482, 497
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 2 cut(s) 390, 466
MluNI TGGCCA 1 cut(s) 44
MlyI GAGTC 2 cut(s) 173, 346
MnlI CCTC 5 cut(s) 97, 223, 250, 543, 588
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
MseI TTAA 1 cut(s) 435
Msp20I TGGCCA 1 cut(s) 44
MspI CCGG 4 cut(s) 108, 233, 387, 417
MspR9I CCNGG 1 cut(s) 233
MwoI GCNNNNNNNGC 3 cut(s) 56, 225, 251
NciI CCSGG 1 cut(s) 233
NcoI CCATGG 1 cut(s) 45
NdeI CATATG 1 cut(s) 29
NdeII GATC 5 cut(s) 18, 165, 286, 294, 448
NlaIII CATG 3 cut(s) 13, 49, 339
NlaIV GGNNCC 2 cut(s) 220, 479
NmuCI GTSAC 2 cut(s) 235, 382
NspV TTCGAA 1 cut(s) 471
PciSI GCTCTTC 1 cut(s) 105
PflFI GACNNNGTC 1 cut(s) 355
PkrI GCNGC 1 cut(s) 152
PleI GAGTC 2 cut(s) 173, 346
PpsI GAGTC 2 cut(s) 173, 346
PshAI GACNNNNGTC 1 cut(s) 183
PspEI GGTNACC 1 cut(s) 235
PspN4I GGNNCC 2 cut(s) 220, 479
PspPI GGNCC 2 cut(s) 218, 597
PsuI RGATCY 1 cut(s) 448
PsyI GACNNNGTC 1 cut(s) 355
SapI GCTCTTC 1 cut(s) 105
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 1 cut(s) 151
Sau3AI GATC 5 cut(s) 18, 165, 286, 294, 448
Sau96I GGNCC 2 cut(s) 218, 597
SchI GAGTC 2 cut(s) 173, 346
ScrFI CCNGG 1 cut(s) 233
SduI GDGCHC 3 cut(s) 81, 482, 497
SetI ASST 5 cut(s) 89, 215, 242, 345, 554
SfaNI GCATC 1 cut(s) 317
SfuI TTCGAA 1 cut(s) 471
Sse9I AATT 2 cut(s) 390, 466
SsiI CCGC 2 cut(s) 132, 150
SspMI CTAG 1 cut(s) 35
StyD4I CCNGG 1 cut(s) 231
StyI CCWWGG 3 cut(s) 45, 222, 561
TaaI ACNGT 2 cut(s) 55, 268
TaqI TCGA 3 cut(s) 81, 329, 471
TasI AATT 2 cut(s) 390, 466
TauI GCSGC 1 cut(s) 153
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 1 cut(s) 81
TseFI GTSAC 2 cut(s) 235, 382
Tsp45I GTSAC 2 cut(s) 235, 382
TspDTI ATGAA 3 cut(s) 26, 411, 499
TspRI CASTG 1 cut(s) 81
Tth111I GACNNNGTC 1 cut(s) 355
XmiI GTMKAC 1 cut(s) 114
XspI CTAG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.