FvH4_3g17790

alpha-amylase subtilisin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003122
Physical Location & Seq
Forward (+)
11312547 .. 11313371
825 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g17790.t1

Sequence Viewer

Length: 630 bp
ATGATGATGTCCCTGAAGTACTTACTTGGAAGCCTGAGCATGATCAGCAGCATATTCATGGCCGTAGAAACCATAAACCAAACATCTCCGGTGTTTGATACTTCTGGAAAGGCACTAAAATCCGGCATAGATTACTACATCAAACCCGCCGGCACCGACAAAGGTGGTCGTTTAACTCTAATAAACCGGGAGCCAAAATTAAGTCCACTCTACGTTGGCCAAGGAAATATCTCTGGCTTGCCTTTTCACTTCAAGCCATTCTTCACCGGAGAAACAGTGGTGAGGGAGAGTAGGGATCAAATGATCATATCCAGTGTTTCTACACATTGCAGGCGGTTTAGTTCATGGTCAATTGTTACAGCTAACGCAGAGGACCAAAGGAAGTTGATTGTTATGCGTGAACCAGCAAGAGGAAAAGTGCCAGATAGTATATACTTCAGGATTACTAGATCACAGCCGGAGGTGGATGGTAATAGCTACATTTTGAGATGGTGTCCGACCGATGTGTGTCCTAATTGTACATTTGCTGATTGTGGAAATATTGGTTCCTTAGTCGCGAGTAGAGGGAAGAGGTTCTTGGCTTTGAATGGTTCTGAACTTCCAGTTGTGTTTGAGAGGGCTCGAGGATGA

Protein Analysis

210

Amino Acids

23.19

Weight (kDa)

9.42

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 31 - 205 7.7e-31 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 152
AccII CGCG 1 cut(s) 557
AciI CCGC 2 cut(s) 147, 334
AclWI GGATC 1 cut(s) 303
AcoI YGGCCR 2 cut(s) 60, 217
AcuI CTGAAG 2 cut(s) 35, 421
AfaI GTAC 2 cut(s) 20, 520
AfiI CCNNNNNNNGG 1 cut(s) 410
AgsI TTSAA 2 cut(s) 253, 586
AjuI GAANNNNNNNTTGG 2 cut(s) 560, 592
AluBI AGCT 2 cut(s) 362, 477
AluI AGCT 2 cut(s) 362, 477
AlwI GGATC 1 cut(s) 303
Ama87I CYCGRG 1 cut(s) 621
AoxI GGCC 2 cut(s) 60, 217
ApeKI GCWGC 1 cut(s) 48
Asp700I GAANNNNTTC 1 cut(s) 572
AspS9I GGNCC 1 cut(s) 373
AsuC2I CCSGG 1 cut(s) 188
AsuHPI GGTGA 2 cut(s) 256, 292
AvaI CYCGRG 1 cut(s) 621
AvaII GGWCC 1 cut(s) 373
BalI TGGCCA 1 cut(s) 219
BanI GGYRCC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 622
BbvI GCAGC 1 cut(s) 60
BccI CCATC 2 cut(s) 461, 483
BceAI ACGGC 1 cut(s) 47
BclI TGATCA 2 cut(s) 42, 303
BcnI CCSGG 1 cut(s) 188
BfaI CTAG 1 cut(s) 447
BisI GCNGC 1 cut(s) 49
BlsI GCNGC 1 cut(s) 50
BmcAI AGTACT 1 cut(s) 20
Bme1390I CCNGG 1 cut(s) 188
Bme18I GGWCC 1 cut(s) 373
BmeT110I CYCGRG 1 cut(s) 621
BmgT120I GGNCC 1 cut(s) 373
BmiI GGNNCC 3 cut(s) 154, 192, 547
BmrFI CCNGG 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 35
BpuMI CCSGG 1 cut(s) 188
BsaJI CCNNGG 1 cut(s) 220
BsaWI WCCGGW 2 cut(s) 88, 266
BsaXI ACNNNNNCTCC 2 cut(s) 261, 291
Bsc4I CCNNNNNNNGG 1 cut(s) 410
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 2 cut(s) 312, 602
Bse3DI GCAATG 1 cut(s) 325
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 1 cut(s) 472
BseLI CCNNNNNNNGG 1 cut(s) 410
BseMI GCAATG 1 cut(s) 325
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 2 cut(s) 312, 602
BseXI GCAGC 1 cut(s) 60
Bsh1236I CGCG 1 cut(s) 557
Bsh1285I CGRYCG 1 cut(s) 501
BshFI GGCC 2 cut(s) 62, 219
BshNI GGYRCC 1 cut(s) 152
BsiEI CGRYCG 1 cut(s) 501
BsiHKCI CYCGRG 1 cut(s) 621
BsiSI CCGG 6 cut(s) 89, 123, 150, 187, 267, 458
BslI CCNNNNNNNGG 1 cut(s) 410
BsnI GGCC 2 cut(s) 62, 219
BsoBI CYCGRG 1 cut(s) 621
Bsp1286I GDGCHC 1 cut(s) 622
Bsp1407I TGTACA 1 cut(s) 518
Bsp143I GATC 4 cut(s) 42, 295, 303, 449
Bsp68I TCGCGA 1 cut(s) 557
BspACI CCGC 2 cut(s) 147, 334
BspANI GGCC 2 cut(s) 62, 219
BspCNI CTCAG 1 cut(s) 27
BspFNI CGCG 1 cut(s) 557
BspLI GGNNCC 3 cut(s) 154, 192, 547
BspPI GGATC 1 cut(s) 303
BspT107I GGYRCC 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 325
BsrFI RCCGGY 1 cut(s) 149
BsrGI TGTACA 1 cut(s) 518
BsrI ACTGG 2 cut(s) 312, 602
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 4 cut(s) 42, 295, 303, 449
BssT1I CCWWGG 1 cut(s) 220
Bst4CI ACNGT 1 cut(s) 277
Bst6I CTCTTC 1 cut(s) 563
BstAUI TGTACA 1 cut(s) 518
BstC8I GCNNGC 3 cut(s) 151, 239, 332
BstDEI CTNAG 2 cut(s) 35, 550
BstF5I GGATG 1 cut(s) 472
BstFNI CGCG 1 cut(s) 557
BstKTI GATC 4 cut(s) 45, 298, 306, 452
BstMBI GATC 4 cut(s) 42, 295, 303, 449
BstMCI CGRYCG 1 cut(s) 501
BstMWI GCNNNNNNNGC 1 cut(s) 45
BstSCI CCNGG 1 cut(s) 186
BstUI CGCG 1 cut(s) 557
BstV1I GCAGC 1 cut(s) 60
BsuRI GGCC 2 cut(s) 62, 219
BtsCI GGATG 1 cut(s) 472
BtsIMutI CAGTG 2 cut(s) 282, 319
BtuMI TCGCGA 1 cut(s) 557
Cac8I GCNNGC 3 cut(s) 151, 239, 332
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 373
Csp6I GTAC 2 cut(s) 19, 519
CviAII CATG 3 cut(s) 40, 58, 345
CviQI GTAC 2 cut(s) 19, 519
DdeI CTNAG 2 cut(s) 35, 550
DpnI GATC 4 cut(s) 44, 297, 305, 451
DpnII GATC 4 cut(s) 42, 295, 303, 449
EaeI YGGCCR 2 cut(s) 60, 217
Eam1104I CTCTTC 1 cut(s) 563
EarI CTCTTC 1 cut(s) 563
Eco130I CCWWGG 1 cut(s) 220
Eco24I GRGCYC 1 cut(s) 622
Eco47I GGWCC 1 cut(s) 373
Eco57I CTGAAG 2 cut(s) 35, 421
Eco88I CYCGRG 1 cut(s) 621
EcoT14I CCWWGG 1 cut(s) 220
EcoT38I GRGCYC 1 cut(s) 622
ErhI CCWWGG 1 cut(s) 220
FaeI CATG 3 cut(s) 43, 61, 348
FalI AAGNNNNNCTT 4 cut(s) 245, 277, 560, 592
FatI CATG 3 cut(s) 39, 57, 344
FauI CCCGC 1 cut(s) 154
FbaI TGATCA 2 cut(s) 42, 303
Fnu4HI GCNGC 1 cut(s) 49
FokI GGATG 1 cut(s) 479
FriOI GRGCYC 1 cut(s) 622
Fsp4HI GCNGC 1 cut(s) 49
FspBI CTAG 1 cut(s) 447
GluI GCNGC 1 cut(s) 49
HaeIII GGCC 2 cut(s) 62, 219
HapII CCGG 6 cut(s) 89, 123, 150, 187, 267, 458
Hin1II CATG 3 cut(s) 43, 61, 348
HpaII CCGG 6 cut(s) 89, 123, 150, 187, 267, 458
HphI GGTGA 2 cut(s) 256, 292
Hpy166II GTNNAC 2 cut(s) 206, 401
Hpy188I TCNGA 2 cut(s) 498, 595
Hpy188III TCNNGA 3 cut(s) 105, 439, 556
Hpy8I GTNNAC 2 cut(s) 206, 401
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 1 cut(s) 330
HpyF10VI GCNNNNNNNGC 1 cut(s) 45
HpyF3I CTNAG 2 cut(s) 35, 550
HpySE526I ACGT 1 cut(s) 213
Hsp92II CATG 3 cut(s) 43, 61, 348
KroI GCCGGC 1 cut(s) 149
KroNI GCCGGC 1 cut(s) 151
Ksp22I TGATCA 2 cut(s) 42, 303
Kzo9I GATC 4 cut(s) 42, 295, 303, 449
LmnI GCTCC 1 cut(s) 190
Lsp1109I GCAGC 1 cut(s) 60
MaeI CTAG 1 cut(s) 447
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 1 cut(s) 355
MalI GATC 4 cut(s) 44, 297, 305, 451
MboI GATC 4 cut(s) 42, 295, 303, 449
MboII GAAGA 2 cut(s) 253, 580
MfeI CAATTG 1 cut(s) 351
MhlI GDGCHC 1 cut(s) 622
MlsI TGGCCA 1 cut(s) 219
MluCI AATT 3 cut(s) 197, 351, 514
MluNI TGGCCA 1 cut(s) 219
MmeI TCCRAC 1 cut(s) 521
MnlI CCTC 8 cut(s) 276, 364, 404, 454, 557, 564, 609, 617
Mox20I TGGCCA 1 cut(s) 219
MroNI GCCGGC 1 cut(s) 149
MroXI GAANNNNTTC 1 cut(s) 572
MscI TGGCCA 1 cut(s) 219
MseI TTAA 2 cut(s) 173, 200
MslI CAYNNNNRTG 1 cut(s) 56
Msp20I TGGCCA 1 cut(s) 219
MspI CCGG 6 cut(s) 89, 123, 150, 187, 267, 458
MspR9I CCNGG 1 cut(s) 188
MunI CAATTG 1 cut(s) 351
MvnI CGCG 1 cut(s) 557
MwoI GCNNNNNNNGC 1 cut(s) 45
NaeI GCCGGC 1 cut(s) 151
NciI CCSGG 1 cut(s) 188
NdeII GATC 4 cut(s) 42, 295, 303, 449
NgoMIV GCCGGC 1 cut(s) 149
NlaIII CATG 3 cut(s) 43, 61, 348
NlaIV GGNNCC 3 cut(s) 154, 192, 547
NruI TCGCGA 1 cut(s) 557
PaeR7I CTCGAG 1 cut(s) 621
PdiI GCCGGC 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 572
PkrI GCNGC 1 cut(s) 50
PspN4I GGNNCC 3 cut(s) 154, 192, 547
PspPI GGNCC 1 cut(s) 373
PspXI VCTCGAGB 1 cut(s) 621
RruI TCGCGA 1 cut(s) 557
RsaI GTAC 2 cut(s) 20, 520
RsaNI GTAC 2 cut(s) 19, 519
RseI CAYNNNNRTG 1 cut(s) 56
SaqAI TTAA 2 cut(s) 173, 200
SatI GCNGC 1 cut(s) 49
Sau3AI GATC 4 cut(s) 42, 295, 303, 449
Sau96I GGNCC 1 cut(s) 373
ScaI AGTACT 1 cut(s) 20
ScrFI CCNGG 1 cut(s) 188
SduI GDGCHC 1 cut(s) 622
SetI ASST 6 cut(s) 166, 216, 364, 465, 479, 575
Sfr274I CTCGAG 1 cut(s) 621
SinI GGWCC 1 cut(s) 373
SlaI CTCGAG 1 cut(s) 621
SmiMI CAYNNNNRTG 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 621
SmoI CTYRAG 1 cut(s) 621
Sse9I AATT 3 cut(s) 197, 351, 514
SsiI CCGC 2 cut(s) 147, 334
SspI AATATT 1 cut(s) 541
SspMI CTAG 1 cut(s) 447
StyD4I CCNGG 1 cut(s) 186
StyI CCWWGG 1 cut(s) 220
TaaI ACNGT 1 cut(s) 277
TaiI ACGT 1 cut(s) 216
TaqI TCGA 1 cut(s) 622
TaqII GACCGA 1 cut(s) 515
TasI AATT 3 cut(s) 197, 351, 514
TatI WGTACW 2 cut(s) 18, 518
Tru1I TTAA 2 cut(s) 173, 200
Tru9I TTAA 2 cut(s) 173, 200
TscAI CASTG 2 cut(s) 282, 319
TseI GCWGC 1 cut(s) 48
TspDTI ATGAA 2 cut(s) 46, 333
TspRI CASTG 2 cut(s) 282, 319
VpaK11BI GGWCC 1 cut(s) 373
XhoI CTCGAG 1 cut(s) 621
XmnI GAANNNNTTC 1 cut(s) 572
XspI CTAG 1 cut(s) 447
ZrmI AGTACT 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.