MD11G1269300.v1.1

alpha-amylase subtilisin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003122
Physical Location & Seq
Reverse (-)
38504739 .. 38505383
645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1269300.v1.1.491

Sequence Viewer

Length: 645 bp
ATGATGTCTATGAAGTTTATTTCAAGCTTCCTCAGCTTCACATTGCTAATGATGGCCATATCGACCCTAGCCCAAACATCAAATGACACAGCAGGTTCTCCGGTGCTCGACACAACCGGACAACCTCTTCAACGTGGGGTTGAGTACTACATCAAGCCTGCCATTACCGAAAATGGCGGCCGTTTCACTTTGATCGACCATAGAAACGGAACATGCCCGTTTTACGTAGGTCAAGAAAACCTCTCAGGTCCCGATGGCTTTCCGGTGACCTTTGCGCCATTCGCAGAAGGTGAAACAGTGGTGAAGGAGGGTAGGGACATAAAAATCACGTTTGCGGTGTCCACAATTTGTGTGCAGTCAACTGCGTGGAAGCGAAGCAATGAGACGGGCCAAGAGACCGAAAGGAGACTAATTGTGATTGGAGAAGACGAAAATGAAAGGTATCCGACTGGAAACTACTTTAAGATTGTTAGAGAAGGGAATGGTCTCTACAGTCTGTTTTGGTGTCCTTATGAGGCGTGTCCCATTTGCAAATTTGATTGTAGAGCGACGTGGGTTGGTGTTTTGGTTGAGAACGGGAAGAGGTTGCTGGCATTGGATGGCTCTGCGCTTCCTGTTGAGTTTGAAAGGCATATTATTGATTAG

Protein Analysis

215

Amino Acids

23.85

Weight (kDa)

5.08

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 35 - 209 2.4e-43 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 83
AciI CCGC 2 cut(s) 177, 335
AcoI YGGCCR 2 cut(s) 54, 178
AcsI RAATTY 1 cut(s) 533
AfaI GTAC 1 cut(s) 146
AgsI TTSAA 3 cut(s) 24, 131, 626
AjiI CACGTC 1 cut(s) 552
AluBI AGCT 2 cut(s) 27, 36
AluI AGCT 2 cut(s) 27, 36
Alw21I GWGCWC 1 cut(s) 108
Alw26I GTCTC 4 cut(s) 377, 389, 400, 491
AoxI GGCC 3 cut(s) 54, 178, 388
ApoI RAATTY 1 cut(s) 533
AspLEI GCGC 2 cut(s) 277, 610
AspS9I GGNCC 2 cut(s) 248, 388
AsuHPI GGTGA 3 cut(s) 277, 302, 313
AvaII GGWCC 1 cut(s) 248
BalI TGGCCA 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 108
BbvCI CCTCAGC 1 cut(s) 32
BccI CCATC 3 cut(s) 46, 248, 593
BceAI ACGGC 1 cut(s) 165
BciVI GTATCC 1 cut(s) 453
BcoDI GTCTC 4 cut(s) 377, 389, 400, 491
BfaI CTAG 1 cut(s) 68
BfmI CTRYAG 1 cut(s) 490
BfuAI ACCTGC 1 cut(s) 83
BfuI GTATCC 1 cut(s) 453
BisI GCNGC 1 cut(s) 178
BlsI GCNGC 1 cut(s) 179
BmcAI AGTACT 1 cut(s) 146
Bme18I GGWCC 1 cut(s) 248
BmgBI CACGTC 1 cut(s) 552
BmgT120I GGNCC 2 cut(s) 248, 388
BmiI GGNNCC 1 cut(s) 250
BpiI GAAGAC 1 cut(s) 432
Bpu10I CCTNAGC 1 cut(s) 32
BsaAI YACGTR 1 cut(s) 226
BsaI GGTCTC 2 cut(s) 389, 491
BsaWI WCCGGW 3 cut(s) 100, 116, 262
Bse1I ACTGG 1 cut(s) 454
Bse3DI GCAATG 2 cut(s) 41, 385
BseGI GGATG 1 cut(s) 604
BseMI GCAATG 2 cut(s) 41, 385
BseMII CTCAG 2 cut(s) 46, 258
BseNI ACTGG 1 cut(s) 454
BseX3I CGGCCG 1 cut(s) 178
BsgI GTGCAG 1 cut(s) 374
Bsh1285I CGRYCG 1 cut(s) 181
BshFI GGCC 3 cut(s) 56, 180, 390
BsiEI CGRYCG 1 cut(s) 181
BsiHKAI GWGCWC 1 cut(s) 108
BsiSI CCGG 3 cut(s) 101, 117, 263
BslFI GGGAC 3 cut(s) 234, 329, 507
BsmAI GTCTC 4 cut(s) 377, 389, 400, 491
BsmBI CGTCTC 1 cut(s) 377
BsmFI GGGAC 3 cut(s) 234, 329, 507
BsnI GGCC 3 cut(s) 56, 180, 390
Bso31I GGTCTC 2 cut(s) 389, 491
Bsp1286I GDGCHC 1 cut(s) 108
Bsp143I GATC 1 cut(s) 192
BspACI CCGC 2 cut(s) 177, 335
BspANI GGCC 3 cut(s) 56, 180, 390
BspCNI CTCAG 2 cut(s) 45, 257
BspLI GGNNCC 1 cut(s) 250
BspMI ACCTGC 1 cut(s) 83
BspTNI GGTCTC 2 cut(s) 389, 491
BsrDI GCAATG 2 cut(s) 41, 385
BsrI ACTGG 1 cut(s) 454
BssMI GATC 1 cut(s) 192
Bst4CI ACNGT 2 cut(s) 298, 494
Bst6I CTCTTC 2 cut(s) 132, 575
BstBAI YACGTR 1 cut(s) 226
BstC8I GCNNGC 2 cut(s) 159, 591
BstDEI CTNAG 2 cut(s) 32, 244
BstEII GGTNACC 1 cut(s) 265
BstF5I GGATG 1 cut(s) 604
BstHHI GCGC 2 cut(s) 277, 610
BstKTI GATC 1 cut(s) 195
BstMAI GTCTC 4 cut(s) 377, 389, 400, 491
BstMBI GATC 1 cut(s) 192
BstMCI CGRYCG 1 cut(s) 181
BstMWI GCNNNNNNNGC 2 cut(s) 33, 281
BstNSI RCATGY 1 cut(s) 216
BstPI GGTNACC 1 cut(s) 265
BstSFI CTRYAG 1 cut(s) 490
BstSNI TACGTA 1 cut(s) 226
BstV2I GAAGAC 1 cut(s) 432
BstZI CGGCCG 1 cut(s) 178
BsuI GTATCC 1 cut(s) 453
BsuRI GGCC 3 cut(s) 56, 180, 390
BtrI CACGTC 1 cut(s) 552
BtsCI GGATG 1 cut(s) 604
BtsIMutI CAGTG 1 cut(s) 303
BveI ACCTGC 1 cut(s) 83
Cac8I GCNNGC 2 cut(s) 159, 591
CfoI GCGC 2 cut(s) 277, 610
Cfr13I GGNCC 2 cut(s) 248, 388
Csp6I GTAC 1 cut(s) 145
CviAII CATG 1 cut(s) 213
CviJI RGCY 9 cut(s) 27, 36, 56, 71, 157, 180, 258, 390, 603
CviKI_1 RGCY 9 cut(s) 27, 36, 56, 71, 157, 180, 258, 390, 603
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 2 cut(s) 32, 244
DpnI GATC 1 cut(s) 194
DpnII GATC 1 cut(s) 192
EaeI YGGCCR 2 cut(s) 54, 178
EagI CGGCCG 1 cut(s) 178
Eam1104I CTCTTC 2 cut(s) 132, 575
EarI CTCTTC 2 cut(s) 132, 575
EclXI CGGCCG 1 cut(s) 178
Eco105I TACGTA 1 cut(s) 226
Eco31I GGTCTC 2 cut(s) 389, 491
Eco47I GGWCC 1 cut(s) 248
Eco52I CGGCCG 1 cut(s) 178
Eco91I GGTNACC 1 cut(s) 265
EcoO109I RGGNCCY 1 cut(s) 248
EcoO65I GGTNACC 1 cut(s) 265
Esp3I CGTCTC 1 cut(s) 377
FaeI CATG 1 cut(s) 216
FaiI YATR 7 cut(s) 11, 59, 201, 214, 320, 513, 633
FaqI GGGAC 3 cut(s) 234, 329, 507
FatI CATG 1 cut(s) 212
Fnu4HI GCNGC 1 cut(s) 178
FokI GGATG 1 cut(s) 611
Fsp4HI GCNGC 1 cut(s) 178
FspBI CTAG 1 cut(s) 68
GlaI GCGC 2 cut(s) 276, 609
GluI GCNGC 1 cut(s) 178
HaeIII GGCC 3 cut(s) 56, 180, 390
HapII CCGG 3 cut(s) 101, 117, 263
HhaI GCGC 2 cut(s) 277, 610
Hin1II CATG 1 cut(s) 216
Hin6I GCGC 2 cut(s) 275, 608
HinP1I GCGC 2 cut(s) 275, 608
HincII GTYRAC 1 cut(s) 360
HindII GTYRAC 1 cut(s) 360
HindIII AAGCTT 1 cut(s) 25
HpaII CCGG 3 cut(s) 101, 117, 263
HphI GGTGA 3 cut(s) 277, 302, 313
Hpy166II GTNNAC 2 cut(s) 342, 360
Hpy188I TCNGA 1 cut(s) 447
Hpy188III TCNNGA 2 cut(s) 233, 251
Hpy8I GTNNAC 2 cut(s) 342, 360
Hpy99I CGWCG 1 cut(s) 553
HpyAV CCTTC 3 cut(s) 281, 298, 470
HpyCH4III ACNGT 2 cut(s) 298, 494
HpyCH4IV ACGT 4 cut(s) 133, 225, 329, 551
HpyCH4V TGCA 2 cut(s) 355, 531
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 281
HpyF3I CTNAG 2 cut(s) 32, 244
HpySE526I ACGT 4 cut(s) 133, 225, 329, 551
Hsp92II CATG 1 cut(s) 216
HspAI GCGC 2 cut(s) 275, 608
Kzo9I GATC 1 cut(s) 192
LpnPI CCDG 9 cut(s) 78, 114, 130, 171, 231, 276, 435, 575, 627
MaeI CTAG 1 cut(s) 68
MaeII ACGT 4 cut(s) 133, 225, 329, 551
MaeIII GTNAC 1 cut(s) 265
MalI GATC 1 cut(s) 194
MboI GATC 1 cut(s) 192
MboII GAAGA 3 cut(s) 119, 437, 592
MhlI GDGCHC 1 cut(s) 108
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 3 cut(s) 345, 411, 533
MluNI TGGCCA 1 cut(s) 56
MmeI TCCRAC 1 cut(s) 470
MnlI CCTC 6 cut(s) 41, 135, 251, 301, 508, 576
Mox20I TGGCCA 1 cut(s) 56
MscI TGGCCA 1 cut(s) 56
MseI TTAA 1 cut(s) 462
Msp20I TGGCCA 1 cut(s) 56
MspI CCGG 3 cut(s) 101, 117, 263
MwoI GCNNNNNNNGC 2 cut(s) 33, 281
NdeII GATC 1 cut(s) 192
NlaIII CATG 1 cut(s) 216
NlaIV GGNNCC 1 cut(s) 250
NmuCI GTSAC 1 cut(s) 265
NspI RCATGY 1 cut(s) 216
PkrI GCNGC 1 cut(s) 179
Ppu21I YACGTR 1 cut(s) 226
PpuMI RGGWCCY 1 cut(s) 248
Psp5II RGGWCCY 1 cut(s) 248
PspEI GGTNACC 1 cut(s) 265
PspN4I GGNNCC 1 cut(s) 250
PspPI GGNCC 2 cut(s) 248, 388
PspPPI RGGWCCY 1 cut(s) 248
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 1 cut(s) 178
Sau3AI GATC 1 cut(s) 192
Sau96I GGNCC 2 cut(s) 248, 388
ScaI AGTACT 1 cut(s) 146
SduI GDGCHC 1 cut(s) 108
SfcI CTRYAG 1 cut(s) 490
SinI GGWCC 1 cut(s) 248
SnaBI TACGTA 1 cut(s) 226
Sse9I AATT 3 cut(s) 345, 411, 533
SsiI CCGC 2 cut(s) 177, 335
SspMI CTAG 1 cut(s) 68
TaaI ACNGT 2 cut(s) 298, 494
TaiI ACGT 4 cut(s) 136, 228, 332, 554
TaqI TCGA 3 cut(s) 62, 108, 195
TaqII GACCGA 1 cut(s) 413
TasI AATT 3 cut(s) 345, 411, 533
TatI WGTACW 1 cut(s) 144
TauI GCSGC 1 cut(s) 180
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TscAI CASTG 1 cut(s) 303
TseFI GTSAC 1 cut(s) 265
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 2 cut(s) 26, 450
TspGWI ACGGA 1 cut(s) 222
TspRI CASTG 1 cut(s) 303
VpaK11BI GGWCC 1 cut(s) 248
XapI RAATTY 1 cut(s) 533
XceI RCATGY 1 cut(s) 216
XspI CTAG 1 cut(s) 68
ZrmI AGTACT 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.