MD03G1251400.v1.1

alpha-amylase subtilisin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003122
Physical Location & Seq
Forward (+)
33912007 .. 33912633
627 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1251400.v1.1.491

Sequence Viewer

Length: 627 bp
ATGTCTATGAAGTTGATTTGGAGCCTCTGCAGCTGCATATGGCTAGTCATGGCCATATCAACCCTAGCCCAAACATCCGATGACTCGAATGCAGTGCTTGACACTGCCGGGCAGGCTCTTCAATCCGGTGTAGACTACTACATCCAACCCGCTATTACCGACAATGGCGGGCGTTTCACTTTGATCAACAGAACTGAATTATGTCCACTTTACGTTGGTCAAGAAAATGTCTCAGGTCCTAAGGGCTTGCCTGTGACATTTTCTCCTTTTGTTGAGGGGGAAACTGTGGTGAGGGAAGGAAGGGACCAAAAGATCACATTCTCAGCAGTCACAATCTGTGTGCAATCAACCACATGGAAGGTGGGTGAGACTGATCAAGACACCCAAAGGAGATTAATTGGCACCGGAGAAGACGAAAACCAAAGCCTTCCTGGCCGTGCTAGAAACTACTTCAGGATTACAAAGCAGGCAACACCAGATGGTGTGTACAATCTGGAATGGTGCCCTACTGAAGTGTGTCCAACTTGTAGGTTCATTTGTGGATCTGTTGGTGCTATTGTTGAGAATGACAAGAGGCTGCTGGCCTTGGATGGTTCAGTGCTTCCTGTTGTGTTTGAGAGGGCTTAA

Protein Analysis

209

Amino Acids

22.82

Weight (kDa)

4.76

Isoelectric Point (pI)

36.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 32 - 206 9.2e-48 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 401, 501
AccI GTMKAC 1 cut(s) 132
AciI CCGC 2 cut(s) 150, 168
AclWI GGATC 1 cut(s) 550
AcoI YGGCCR 2 cut(s) 51, 433
AcuI CTGAAG 2 cut(s) 436, 531
AfaI GTAC 1 cut(s) 488
AgsI TTSAA 1 cut(s) 122
AjnI CCWGG 1 cut(s) 430
AluBI AGCT 1 cut(s) 33
AluI AGCT 1 cut(s) 33
Alw26I GTCTC 2 cut(s) 235, 362
AlwI GGATC 1 cut(s) 550
AoxI GGCC 3 cut(s) 51, 433, 582
ApeKI GCWGC 3 cut(s) 30, 33, 577
AseI ATTAAT 1 cut(s) 395
AspS9I GGNCC 2 cut(s) 236, 304
AsuC2I CCSGG 1 cut(s) 109
AsuHPI GGTGA 2 cut(s) 301, 377
AvaII GGWCC 2 cut(s) 236, 304
AxyI CCTNAGG 1 cut(s) 240
BaeGI GKGCMC 1 cut(s) 506
BalI TGGCCA 1 cut(s) 53
BanI GGYRCC 2 cut(s) 401, 501
BbsI GAAGAC 1 cut(s) 417
BbvI GCAGC 3 cut(s) 20, 42, 564
BccI CCATC 2 cut(s) 473, 584
BceAI ACGGC 1 cut(s) 420
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BciT130I CCWGG 1 cut(s) 432
BclI TGATCA 2 cut(s) 183, 373
BcnI CCSGG 1 cut(s) 109
BcoDI GTCTC 2 cut(s) 235, 362
BfaI CTAG 3 cut(s) 44, 65, 441
BfmI CTRYAG 1 cut(s) 28
BglI GCCNNNNNGGC 2 cut(s) 113, 432
BisI GCNGC 3 cut(s) 31, 34, 578
BlsI GCNGC 3 cut(s) 32, 35, 579
Bme1390I CCNGG 2 cut(s) 109, 432
Bme18I GGWCC 2 cut(s) 236, 304
BmgT120I GGNCC 2 cut(s) 236, 304
BmiI GGNNCC 4 cut(s) 23, 305, 403, 503
BmrFI CCNGG 2 cut(s) 109, 432
BpiI GAAGAC 1 cut(s) 417
BpuMI CCSGG 1 cut(s) 109
BsaJI CCNNGG 1 cut(s) 585
BsaWI WCCGGW 2 cut(s) 125, 404
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bse21I CCTNAGG 1 cut(s) 240
BseBI CCWGG 1 cut(s) 432
BseDI CCNNGG 1 cut(s) 585
BseGI GGATG 3 cut(s) 74, 141, 595
BseMII CTCAG 2 cut(s) 246, 336
BseSI GKGCMC 1 cut(s) 506
BseXI GCAGC 3 cut(s) 20, 42, 564
BshFI GGCC 3 cut(s) 53, 435, 584
BshNI GGYRCC 2 cut(s) 401, 501
BsiSI CCGG 3 cut(s) 108, 126, 405
BslFI GGGAC 1 cut(s) 317
BsmAI GTCTC 2 cut(s) 235, 362
BsmFI GGGAC 1 cut(s) 317
BsmI GAATGC 1 cut(s) 94
BsnI GGCC 3 cut(s) 53, 435, 584
Bsp1286I GDGCHC 1 cut(s) 506
Bsp1407I TGTACA 1 cut(s) 486
Bsp143I GATC 4 cut(s) 183, 312, 373, 542
BspACI CCGC 2 cut(s) 150, 168
BspANI GGCC 3 cut(s) 53, 435, 584
BspCNI CTCAG 2 cut(s) 245, 335
BspLI GGNNCC 4 cut(s) 23, 305, 403, 503
BspMAI CTGCAG 1 cut(s) 32
BspPI GGATC 1 cut(s) 550
BspQI GCTCTTC 1 cut(s) 123
BspT107I GGYRCC 2 cut(s) 401, 501
BsrGI TGTACA 1 cut(s) 486
BssECI CCNNGG 1 cut(s) 585
BssMI GATC 4 cut(s) 183, 312, 373, 542
BssT1I CCWWGG 1 cut(s) 585
Bst2UI CCWGG 1 cut(s) 432
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 1 cut(s) 123
BstAUI TGTACA 1 cut(s) 486
BstC8I GCNNGC 5 cut(s) 114, 170, 248, 468, 582
BstDEI CTNAG 3 cut(s) 232, 240, 322
BstF5I GGATG 3 cut(s) 74, 141, 595
BstKTI GATC 4 cut(s) 186, 315, 376, 545
BstMAI GTCTC 2 cut(s) 235, 362
BstMBI GATC 4 cut(s) 183, 312, 373, 542
BstMWI GCNNNNNNNGC 3 cut(s) 30, 113, 432
BstNI CCWGG 1 cut(s) 432
BstSCI CCNGG 2 cut(s) 107, 430
BstSFI CTRYAG 1 cut(s) 28
BstSLI GKGCMC 1 cut(s) 506
BstV1I GCAGC 3 cut(s) 20, 42, 564
BstV2I GAAGAC 1 cut(s) 417
BstX2I RGATCY 1 cut(s) 542
BstYI RGATCY 1 cut(s) 542
Bsu36I CCTNAGG 1 cut(s) 240
BsuRI GGCC 3 cut(s) 53, 435, 584
BtsCI GGATG 3 cut(s) 74, 141, 595
BtsI GCAGTG 2 cut(s) 99, 102
BtsIMutI CAGTG 3 cut(s) 99, 102, 603
Cac8I GCNNGC 5 cut(s) 114, 170, 248, 468, 582
Cfr13I GGNCC 2 cut(s) 236, 304
Csp6I GTAC 1 cut(s) 487
CviAII CATG 2 cut(s) 49, 354
CviQI GTAC 1 cut(s) 487
DdeI CTNAG 3 cut(s) 232, 240, 322
DpnI GATC 4 cut(s) 185, 314, 375, 544
DpnII GATC 4 cut(s) 183, 312, 373, 542
EaeI YGGCCR 2 cut(s) 51, 433
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco130I CCWWGG 1 cut(s) 585
Eco47I GGWCC 2 cut(s) 236, 304
Eco57I CTGAAG 2 cut(s) 436, 531
Eco81I CCTNAGG 1 cut(s) 240
EcoO109I RGGNCCY 1 cut(s) 236
EcoRII CCWGG 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 585
ErhI CCWWGG 1 cut(s) 585
FaeI CATG 2 cut(s) 52, 357
FaiI YATR 7 cut(s) 8, 38, 40, 50, 56, 202, 355
FaqI GGGAC 1 cut(s) 317
FatI CATG 2 cut(s) 48, 353
FauI CCCGC 2 cut(s) 157, 161
FauNDI CATATG 1 cut(s) 38
FbaI TGATCA 2 cut(s) 183, 373
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 3 cut(s) 31, 34, 578
FokI GGATG 3 cut(s) 61, 128, 602
Fsp4HI GCNGC 3 cut(s) 31, 34, 578
FspBI CTAG 3 cut(s) 44, 65, 441
GluI GCNGC 3 cut(s) 31, 34, 578
HaeIII GGCC 3 cut(s) 53, 435, 584
HapII CCGG 3 cut(s) 108, 126, 405
Hin1II CATG 2 cut(s) 52, 357
HinfI GANTC 1 cut(s) 83
HpaII CCGG 3 cut(s) 108, 126, 405
HphI GGTGA 2 cut(s) 301, 377
Hpy166II GTNNAC 3 cut(s) 133, 206, 487
Hpy188I TCNGA 1 cut(s) 79
Hpy188III TCNNGA 4 cut(s) 221, 377, 454, 494
Hpy8I GTNNAC 3 cut(s) 133, 206, 487
HpyAV CCTTC 4 cut(s) 290, 294, 352, 437
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 4 cut(s) 30, 36, 92, 343
HpyF10VI GCNNNNNNNGC 3 cut(s) 30, 113, 432
HpyF3I CTNAG 3 cut(s) 232, 240, 322
HpySE526I ACGT 1 cut(s) 213
Hsp92II CATG 2 cut(s) 52, 357
Ksp22I TGATCA 2 cut(s) 183, 373
Kzo9I GATC 4 cut(s) 183, 312, 373, 542
LguI GCTCTTC 1 cut(s) 123
LmnI GCTCC 1 cut(s) 21
Lsp1109I GCAGC 3 cut(s) 20, 42, 564
MaeI CTAG 3 cut(s) 44, 65, 441
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 2 cut(s) 253, 328
MalI GATC 4 cut(s) 185, 314, 375, 544
MboI GATC 4 cut(s) 183, 312, 373, 542
MboII GAAGA 2 cut(s) 110, 422
MflI RGATCY 1 cut(s) 542
MhlI GDGCHC 1 cut(s) 506
MlsI TGGCCA 1 cut(s) 53
MluCI AATT 2 cut(s) 197, 396
MluNI TGGCCA 1 cut(s) 53
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 2 cut(s) 169, 545
MnlI CCTC 5 cut(s) 35, 268, 285, 567, 612
Mox20I TGGCCA 1 cut(s) 53
MscI TGGCCA 1 cut(s) 53
MseI TTAA 2 cut(s) 395, 625
Msp20I TGGCCA 1 cut(s) 53
MspA1I CMGCKG 1 cut(s) 33
MspI CCGG 3 cut(s) 108, 126, 405
MspR9I CCNGG 2 cut(s) 109, 432
Mva1269I GAATGC 1 cut(s) 94
MvaI CCWGG 1 cut(s) 432
MwoI GCNNNNNNNGC 3 cut(s) 30, 113, 432
NciI CCSGG 1 cut(s) 109
NdeI CATATG 1 cut(s) 38
NdeII GATC 4 cut(s) 183, 312, 373, 542
NlaIII CATG 2 cut(s) 52, 357
NlaIV GGNNCC 4 cut(s) 23, 305, 403, 503
NmuCI GTSAC 2 cut(s) 253, 328
PciSI GCTCTTC 1 cut(s) 123
PctI GAATGC 1 cut(s) 94
PkrI GCNGC 3 cut(s) 32, 35, 579
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
PpuMI RGGWCCY 1 cut(s) 236
PshBI ATTAAT 1 cut(s) 395
Psp5II RGGWCCY 1 cut(s) 236
Psp6I CCWGG 1 cut(s) 430
PspGI CCWGG 1 cut(s) 430
PspN4I GGNNCC 4 cut(s) 23, 305, 403, 503
PspPI GGNCC 2 cut(s) 236, 304
PspPPI RGGWCCY 1 cut(s) 236
PstI CTGCAG 1 cut(s) 32
PsuI RGATCY 1 cut(s) 542
PvuII CAGCTG 1 cut(s) 33
RsaI GTAC 1 cut(s) 488
RsaNI GTAC 1 cut(s) 487
SapI GCTCTTC 1 cut(s) 123
SaqAI TTAA 2 cut(s) 395, 625
SatI GCNGC 3 cut(s) 31, 34, 578
Sau3AI GATC 4 cut(s) 183, 312, 373, 542
Sau96I GGNCC 2 cut(s) 236, 304
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 109, 432
SduI GDGCHC 1 cut(s) 506
SetI ASST 5 cut(s) 35, 216, 238, 363, 533
SfcI CTRYAG 1 cut(s) 28
SinI GGWCC 2 cut(s) 236, 304
Sse9I AATT 2 cut(s) 197, 396
SsiI CCGC 2 cut(s) 150, 168
SspMI CTAG 3 cut(s) 44, 65, 441
StyD4I CCNGG 2 cut(s) 107, 430
StyI CCWWGG 1 cut(s) 585
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 1 cut(s) 216
TaqI TCGA 1 cut(s) 86
TasI AATT 2 cut(s) 197, 396
TatI WGTACW 1 cut(s) 486
Tru1I TTAA 2 cut(s) 395, 625
Tru9I TTAA 2 cut(s) 395, 625
TscAI CASTG 3 cut(s) 99, 109, 603
TseFI GTSAC 2 cut(s) 253, 328
TseI GCWGC 3 cut(s) 30, 33, 577
Tsp45I GTSAC 2 cut(s) 253, 328
TspDTI ATGAA 2 cut(s) 23, 523
TspRI CASTG 3 cut(s) 99, 109, 603
VpaK11BI GGWCC 2 cut(s) 236, 304
VspI ATTAAT 1 cut(s) 395
XcmI CCANNNNNNNNNTGG 2 cut(s) 358, 428
XmiI GTMKAC 1 cut(s) 132
XspI CTAG 3 cut(s) 44, 65, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.