Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0003122

Physical Location & Seq

Genomic Coordinates Reverse (-)

23682832 .. 23687873

Gene Structure 5042 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000033135

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGAAGTCCATGAATTTGATCGGAAGCTTGAGCTGTTGCATGTGGCTAGTGATGGCCATGGCAACCGCAGCCCAAGATAGCGCTCCAGTGCTCGACACCTCTGGGCAGGCTCTTCAATCTGGTGTAGACTACTACATCAAACCCGCCATCACCGACAACGGCGGCCGTTTCACTTTGATCGACCGAAACGACTCGTGTCCTTTCTATGCTGGTCAAGAAAACACCTCGGGCCCCGAGGGCTTCCCGGTGACCTTTGCGCCTTTCGCAGAGGGGGAGACGGTGGTGAGGGAGACTAGGGATCAGAAGATCACATTCACTGCATTCACAATCTGTGTGCAGTCAACGGCATGGAAGGTGGGTGAGACTCAGTCAGAGACTCAGAGGAGATTGATTGTCACCGGAATTGATCAAAACGAAGGCCTTCCAGGTCCGGCCAGAAACTACTTCAGGATTAATAAACAGGCAGATCATGATGGCGTCTACAACCTGCAGTGGTGCCCTACAGAAGTGTGCCCGACATGCAGGTTCATTTGCGGAGATGTGGGTGCTTTGGTTGAGAATGACAAGAGGCTGTTGGCCTTGGATGAATTAGCTAAAAATCCCATAACCACCATCCCACCCAGATACCTACAGCAAGATTACAATGATGGTGATGCTCAAGACCAACTGAACAAGATCAATACCGATGAGACTCGCAGTTCTGTACAACCTGAAATACCATTCATTGACTTCATGAAACTGCTCTCCCAGCAAGAATCAAACAATTTTGATGAACTATCTAGGCTCCACTTAGCTTGCAAAGAGTGGGGTTTCTTCCAGCTGGTAAACCATGGTGTGAGCTCTTCTTTGATGGAGAAAATGAAGACAGAAATTCAAGACTTCTTCAACCTCCCGATAGAGGAGAAGAAAAGGTTTTGGCAGCACCCGGGAGACGTGGAGGGTTTGGGACAGGCCTTTGTCGTTTCTGAAGAGCAAAAACTTGATTGGGCTGACATTTTCTTCCTCACCACCCTTCCTGTCCAGATGAGGAAGCCTTACTTGTTCCCGGAGCTCCCTACTCCTTTCAAAGACACCTTGGAGATTTACTCAATGGAATTGGAAAACCTAGCCATGGCTATCCTAACCCAAATGGAAAATGCTTTAAAAATTGAAGCCAAGGAATTGACCAAGTCGTTTGTAGGTGGAATGCAATCCATGAGGATGAACTATTATCCTCCATGTCCACAGCCGGAGAAAGTCATCGGCCTTACTCCTCATTCGGACGCCGTAGGTCTAACCATCCTCCTGCAAGTCAATGAAATATTGGAAGGTCTCCAAGTCAAGAAGGATGGGATTTGGTTTCCTGTTAAGCCACTTCCAGATGCCTTCATTGTCAACGTCGGAGACATTTTAGAGATTCAAACAAATGGAACTTATCGTAGCATCGAGCATCGAGCAATTGTCAACTCTAAAAAGGAAAGACTGTCAATCGCGACATTTTACAGCCCGAGACTTGATGGCGAAATTTGTCCAGCACCTAGCTTGATTACTGAAGAAACACATGCTGCATATACAAGCATCGGAGTGCAAGATTTCTTCACAGCCTTATTTGCACGTAAGCTTCAAGGAAAATCTTTTCTTGATGATCTTAGATTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0010150 GO:0016491 GO:0016679 GO:0016682 GO:0032501 GO:0032502 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0055114 GO:0090693 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

546

Amino Acids

61.34

Weight (kDa)

4.86

Isoelectric Point (pI)

47.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kunitz_legume	PF00197	30 - 196	9.5e-44	Trypsin and protease inhibitor
DIOX_N	PF14226	239 - 345	2.4e-28	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	397 - 496	9e-33	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003122)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73260
fragaria_vesca	FvH4_3g17480 FvH4_3g17480 FvH4_3g17790
malus_domestica	MD03G1251400.v1.1 MD11G1269300.v1.1 MD11G1269400.v1.1
prunus_persica	Prupe.4G155200_v2.0.a1
pyrus_communis	pycom03g19880 pycom03g19890
rosa_chinensis	RchiOBHm_Chr5g0029031 RchiOBHm_Chr5g0029081
rosa_laevigata	RLG00000033135 RLG00000033138 RLG00000033181
rosa_multiflora	Rmu_co8286077.1_g000001 Rmu_sc0001608.1_g000003 Rmu_sc0004324.1_g000002 Rmu_sc0010962.1_g000005 Rmu_sc0026501.1_g000002
rosa_roxburghii	Rroxscaffold_1G00050360
rosa_rugosa	Rorug05G0112300 Rorug05G0112400
rosa_samantha	Rh5AG204500 Rh5AG204600 Rh5AG207300 Rh5BG202800 Rh5BG203000 Rh5BG206100 Rh5CG224100 Rh5CG228200 Rh5DG206700 Rh5DG206800 Rh5DG209800
rosa_wichuraiana	Rw5G018540 Rw5G018560 Rw5G018860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1636
Acc36I	ACCTGC	2 cut(s)	495, 513
AccB1I	GGYRCC	1 cut(s)	495
AccI	GTMKAC	2 cut(s)	126, 480
AccII	CGCG	1 cut(s)	1472
AciI	CCGC	4 cut(s)	66, 144, 162, 534
AclWI	GGATC	1 cut(s)	306
AcoI	YGGCCR	3 cut(s)	54, 163, 432
AcsI	RAATTY	3 cut(s)	13, 870, 1503
AcuI	CTGAAG	3 cut(s)	430, 987, 1551
AcyI	GRCGYC	2 cut(s)	477, 1263
AfaI	GTAC	1 cut(s)	705
AfeI	AGCGCT	1 cut(s)	82
AfiI	CCNNNNNNNGG	2 cut(s)	898, 1111
AgsI	TTSAA	7 cut(s)	116, 875, 886, 1066, 1151, 1400, 1604
AjiI	CACGTC	1 cut(s)	934
AjnI	CCWGG	1 cut(s)	424
AluBI	AGCT	9 cut(s)	27, 33, 593, 794, 820, 840, 1051, 1521, 1600
AluI	AGCT	9 cut(s)	27, 33, 593, 794, 820, 840, 1051, 1521, 1600
Alw21I	GWGCWC	3 cut(s)	93, 842, 1053
Alw26I	GTCTC	9 cut(s)	269, 284, 356, 368, 683, 924, 1316, 1377, 1483
AlwI	GGATC	1 cut(s)	306
Ama87I	CYCGRG	4 cut(s)	226, 233, 925, 1486
Aor51HI	AGCGCT	1 cut(s)	82
AoxI	GGCC	8 cut(s)	54, 163, 229, 418, 432, 576, 951, 1243
ApaI	GGGCCC	1 cut(s)	233
ApeKI	GCWGC	3 cut(s)	68, 919, 1544
ApoI	RAATTY	3 cut(s)	13, 870, 1503
AseI	ATTAAT	1 cut(s)	453
Asp700I	GAANNNNTTC	1 cut(s)	420
AspLEI	GCGC	2 cut(s)	83, 259
AspS9I	GGNCC	3 cut(s)	229, 230, 428
AsuC2I	CCSGG	4 cut(s)	245, 926, 927, 1046
AsuHPI	GGTGA	7 cut(s)	142, 259, 295, 371, 388, 662, 997
AvaI	CYCGRG	4 cut(s)	226, 233, 925, 1486
AvaII	GGWCC	1 cut(s)	428
BaeGI	GKGCMC	3 cut(s)	233, 500, 515
BalI	TGGCCA	1 cut(s)	56
BanI	GGYRCC	1 cut(s)	495
BanII	GRGCYC	3 cut(s)	233, 842, 1053
BauI	CACGAG	1 cut(s)	193
BbsI	GAAGAC	1 cut(s)	869
Bbv12I	GWGCWC	3 cut(s)	93, 842, 1053
BbvI	GCAGC	3 cut(s)	80, 931, 1531
BccI	CCATC	9 cut(s)	46, 155, 467, 620, 641, 844, 1286, 1322, 1490
BceAI	ACGGC	4 cut(s)	150, 175, 360, 1250
BciT130I	CCWGG	1 cut(s)	426
BclI	TGATCA	1 cut(s)	406
BcnI	CCSGG	4 cut(s)	245, 926, 927, 1046
BcoDI	GTCTC	9 cut(s)	269, 284, 356, 368, 683, 924, 1316, 1377, 1483
BfaI	CTAG	5 cut(s)	47, 294, 780, 1106, 1518
BfmI	CTRYAG	3 cut(s)	488, 501, 629
BfoI	RGCGCY	1 cut(s)	84
BfuAI	ACCTGC	2 cut(s)	495, 513
BglI	GCCNNNNNGGC	1 cut(s)	237
BisI	GCNGC	4 cut(s)	69, 163, 920, 1545
BlsI	GCNGC	4 cut(s)	70, 164, 921, 1546
Bme1390I	CCNGG	5 cut(s)	245, 426, 926, 927, 1046
Bme18I	GGWCC	1 cut(s)	428
BmeT110I	CYCGRG	4 cut(s)	226, 233, 925, 1486
BmgBI	CACGTC	1 cut(s)	934
BmgT120I	GGNCC	3 cut(s)	229, 230, 428
BmiI	GGNNCC	4 cut(s)	231, 232, 497, 785
BmrFI	CCNGG	5 cut(s)	245, 426, 926, 927, 1046
BmsI	GCATC	5 cut(s)	643, 1351, 1431, 1438, 1566
BoxI	GACNNNNGTC	1 cut(s)	195
BpiI	GAAGAC	1 cut(s)	869
BplI	GAGNNNNNCTC	2 cut(s)	1070, 1102
BpmI	CTGGAG	1 cut(s)	69
BpuEI	CTTGAG	2 cut(s)	49, 642
BpuMI	CCSGG	4 cut(s)	245, 926, 927, 1046
BsaAI	YACGTR	1 cut(s)	1595
BsaHI	GRCGYC	2 cut(s)	477, 1263
BsaI	GGTCTC	1 cut(s)	1316
BsaJI	CCNNGG	9 cut(s)	57, 225, 234, 579, 829, 925, 1074, 1110, 1155
BsaWI	WCCGGW	1 cut(s)	398
Bsc4I	CCNNNNNNNGG	2 cut(s)	898, 1111
Bse1I	ACTGG	1 cut(s)	86
BseBI	CCWGG	1 cut(s)	426
BseDI	CCNNGG	9 cut(s)	57, 225, 234, 579, 829, 925, 1074, 1110, 1155
BseGI	GGATG	5 cut(s)	589, 612, 1206, 1278, 1333
BseLI	CCNNNNNNNGG	2 cut(s)	898, 1111
BseMII	CTCAG	2 cut(s)	380, 392
BseNI	ACTGG	1 cut(s)	86
BseRI	GAGGAG	3 cut(s)	397, 914, 1242
BseSI	GKGCMC	3 cut(s)	233, 500, 515
BseX3I	CGGCCG	1 cut(s)	163
BseXI	GCAGC	3 cut(s)	80, 931, 1531
BseYI	CCCAGC	1 cut(s)	747
BsgI	GTGCAG	1 cut(s)	356
Bsh1236I	CGCG	1 cut(s)	1472
Bsh1285I	CGRYCG	2 cut(s)	166, 184
BshFI	GGCC	8 cut(s)	56, 165, 231, 420, 434, 578, 953, 1245
BshNI	GGYRCC	1 cut(s)	495
BsiEI	CGRYCG	2 cut(s)	166, 184
BsiHKAI	GWGCWC	3 cut(s)	93, 842, 1053
BsiHKCI	CYCGRG	4 cut(s)	226, 233, 925, 1486
BsiSI	CCGG	6 cut(s)	245, 399, 431, 926, 1046, 1229
BslFI	GGGAC	1 cut(s)	960
BslI	CCNNNNNNNGG	2 cut(s)	898, 1111
BsmAI	GTCTC	9 cut(s)	269, 284, 356, 368, 683, 924, 1316, 1377, 1483
BsmBI	CGTCTC	2 cut(s)	269, 924
BsmFI	GGGAC	1 cut(s)	960
BsmI	GAATGC	2 cut(s)	320, 1191
BsnI	GGCC	8 cut(s)	56, 165, 231, 420, 434, 578, 953, 1245
Bso31I	GGTCTC	1 cut(s)	1316
BsoBI	CYCGRG	4 cut(s)	226, 233, 925, 1486
Bsp120I	GGGCCC	1 cut(s)	229
Bsp1286I	GDGCHC	6 cut(s)	93, 233, 500, 515, 842, 1053
Bsp1407I	TGTACA	1 cut(s)	703
Bsp143I	GATC	8 cut(s)	18, 177, 298, 306, 406, 466, 675, 1624
Bsp19I	CCATGG	3 cut(s)	57, 829, 1110
Bsp68I	TCGCGA	1 cut(s)	1472
BspACI	CCGC	4 cut(s)	66, 144, 162, 534
BspANI	GGCC	8 cut(s)	56, 165, 231, 420, 434, 578, 953, 1245
BspCNI	CTCAG	2 cut(s)	379, 391
BspFNI	CGCG	1 cut(s)	1472
BspHI	TCATGA	2 cut(s)	469, 732
BspLI	GGNNCC	4 cut(s)	231, 232, 497, 785
BspMAI	CTGCAG	1 cut(s)	492
BspMI	ACCTGC	2 cut(s)	495, 513
BspPI	GGATC	1 cut(s)	306
BspQI	GCTCTTC	3 cut(s)	117, 847, 963
BspT107I	GGYRCC	1 cut(s)	495
BspTNI	GGTCTC	1 cut(s)	1316
BsrGI	TGTACA	1 cut(s)	703
BsrI	ACTGG	1 cut(s)	86
BssECI	CCNNGG	9 cut(s)	57, 225, 234, 579, 829, 925, 1074, 1110, 1155
BssMI	GATC	8 cut(s)	18, 177, 298, 306, 406, 466, 675, 1624
BssNI	GRCGYC	2 cut(s)	477, 1263
BssSI	CACGAG	1 cut(s)	193
BssT1I	CCWWGG	6 cut(s)	57, 579, 829, 1074, 1110, 1155
Bst2BI	CACGAG	1 cut(s)	193
Bst2UI	CCWGG	1 cut(s)	426
Bst4CI	ACNGT	2 cut(s)	280, 1464
Bst6I	CTCTTC	3 cut(s)	117, 847, 963
BstACI	GRCGYC	2 cut(s)	477, 1263
BstAUI	TGTACA	1 cut(s)	703
BstBAI	YACGTR	1 cut(s)	1595
BstC8I	GCNNGC	2 cut(s)	108, 796
BstDEI	CTNAG	4 cut(s)	366, 378, 790, 1628
BstDSI	CCRYGG	3 cut(s)	57, 829, 1110
BstEII	GGTNACC	1 cut(s)	247
BstF5I	GGATG	5 cut(s)	589, 612, 1206, 1278, 1333
BstFNI	CGCG	1 cut(s)	1472
BstH2I	RGCGCY	1 cut(s)	84
BstHHI	GCGC	2 cut(s)	83, 259
BstKTI	GATC	8 cut(s)	21, 180, 301, 309, 409, 469, 678, 1627
BstMAI	GTCTC	9 cut(s)	269, 284, 356, 368, 683, 924, 1316, 1377, 1483
BstMBI	GATC	8 cut(s)	18, 177, 298, 306, 406, 466, 675, 1624
BstMCI	CGRYCG	2 cut(s)	166, 184
BstMWI	GCNNNNNNNGC	6 cut(s)	68, 237, 263, 519, 748, 1589
BstNI	CCWGG	1 cut(s)	426
BstNSI	RCATGY	3 cut(s)	43, 522, 1544
BstPAI	GACNNNNGTC	1 cut(s)	195
BstPI	GGTNACC	1 cut(s)	247
BstSCI	CCNGG	5 cut(s)	243, 424, 924, 925, 1044
BstSFI	CTRYAG	3 cut(s)	488, 501, 629
BstSLI	GKGCMC	3 cut(s)	233, 500, 515
BstUI	CGCG	1 cut(s)	1472
BstV1I	GCAGC	3 cut(s)	80, 931, 1531
BstV2I	GAAGAC	1 cut(s)	869
BstZI	CGGCCG	1 cut(s)	163
BsuRI	GGCC	8 cut(s)	56, 165, 231, 420, 434, 578, 953, 1245
BtgI	CCRYGG	3 cut(s)	57, 829, 1110
BtrI	CACGTC	1 cut(s)	934
BtsCI	GGATG	5 cut(s)	589, 612, 1206, 1278, 1333
BtsI	GCAGTG	2 cut(s)	315, 497
BtsIMutI	CAGTG	3 cut(s)	93, 315, 497
BtuMI	TCGCGA	1 cut(s)	1472
BveI	ACCTGC	2 cut(s)	495, 513
Cac8I	GCNNGC	2 cut(s)	108, 796
CciI	TCATGA	2 cut(s)	469, 732
CfoI	GCGC	2 cut(s)	83, 259
Cfr13I	GGNCC	3 cut(s)	229, 230, 428
Cfr9I	CCCGGG	1 cut(s)	925
CseI	GACGC	2 cut(s)	466, 1271
Csp6I	GTAC	1 cut(s)	704
CspCI	CAANNNNNGTGG	2 cut(s)	776, 811
CviQI	GTAC	1 cut(s)	704
DdeI	CTNAG	4 cut(s)	366, 378, 790, 1628
DpnI	GATC	8 cut(s)	20, 179, 300, 308, 408, 468, 677, 1626
DpnII	GATC	8 cut(s)	18, 177, 298, 306, 406, 466, 675, 1624
DraI	TTTAAA	1 cut(s)	1143
EaeI	YGGCCR	3 cut(s)	54, 163, 432
EagI	CGGCCG	1 cut(s)	163
Eam1104I	CTCTTC	3 cut(s)	117, 847, 963
EarI	CTCTTC	3 cut(s)	117, 847, 963
Ecl136II	GAGCTC	2 cut(s)	840, 1051
EclXI	CGGCCG	1 cut(s)	163
Eco130I	CCWWGG	6 cut(s)	57, 579, 829, 1074, 1110, 1155
Eco147I	AGGCCT	2 cut(s)	420, 953
Eco24I	GRGCYC	3 cut(s)	233, 842, 1053
Eco31I	GGTCTC	1 cut(s)	1316
Eco47I	GGWCC	1 cut(s)	428
Eco47III	AGCGCT	1 cut(s)	82
Eco52I	CGGCCG	1 cut(s)	163
Eco53kI	GAGCTC	2 cut(s)	840, 1051
Eco57I	CTGAAG	3 cut(s)	430, 987, 1551
Eco88I	CYCGRG	4 cut(s)	226, 233, 925, 1486
Eco91I	GGTNACC	1 cut(s)	247
EcoICRI	GAGCTC	2 cut(s)	840, 1051
EcoO109I	RGGNCCY	1 cut(s)	230
EcoO65I	GGTNACC	1 cut(s)	247
EcoRII	CCWGG	1 cut(s)	424
EcoT14I	CCWWGG	6 cut(s)	57, 579, 829, 1074, 1110, 1155
EcoT38I	GRGCYC	3 cut(s)	233, 842, 1053
ErhI	CCWWGG	6 cut(s)	57, 579, 829, 1074, 1110, 1155
Esp3I	CGTCTC	2 cut(s)	269, 924
FalI	AAGNNNNNCTT	2 cut(s)	1022, 1054
FaqI	GGGAC	1 cut(s)	960
FauI	CCCGC	1 cut(s)	151
FbaI	TGATCA	1 cut(s)	406
FblI	GTMKAC	2 cut(s)	126, 480
Fnu4HI	GCNGC	4 cut(s)	69, 163, 920, 1545
FokI	GGATG	5 cut(s)	596, 599, 1213, 1265, 1340
FriOI	GRGCYC	3 cut(s)	233, 842, 1053
Fsp4HI	GCNGC	4 cut(s)	69, 163, 920, 1545
FspBI	CTAG	5 cut(s)	47, 294, 780, 1106, 1518
GlaI	GCGC	2 cut(s)	82, 258
GluI	GCNGC	4 cut(s)	69, 163, 920, 1545
GsaI	CCCAGC	1 cut(s)	751
GsuI	CTGGAG	1 cut(s)	69
HaeII	RGCGCY	1 cut(s)	84
HaeIII	GGCC	8 cut(s)	56, 165, 231, 420, 434, 578, 953, 1245
HapII	CCGG	6 cut(s)	245, 399, 431, 926, 1046, 1229
HgaI	GACGC	2 cut(s)	466, 1271
HhaI	GCGC	2 cut(s)	83, 259
Hin1I	GRCGYC	2 cut(s)	477, 1263
Hin6I	GCGC	2 cut(s)	81, 257
HinP1I	GCGC	2 cut(s)	81, 257
HincII	GTYRAC	3 cut(s)	342, 1375, 1444
HindII	GTYRAC	3 cut(s)	342, 1375, 1444
HindIII	AAGCTT	2 cut(s)	25, 1598
HinfI	GANTC	6 cut(s)	191, 364, 376, 691, 755, 1396
HpaII	CCGG	6 cut(s)	245, 399, 431, 926, 1046, 1229
HphI	GGTGA	7 cut(s)	142, 259, 295, 371, 388, 662, 997
Hpy166II	GTNNAC	7 cut(s)	127, 342, 481, 826, 1223, 1375, 1444
Hpy188I	TCNGA	8 cut(s)	23, 303, 373, 381, 967, 1261, 1382, 1562
Hpy8I	GTNNAC	7 cut(s)	127, 342, 481, 826, 1223, 1375, 1444
Hpy99I	CGWCG	1 cut(s)	1382
HpyAV	CCTTC	7 cut(s)	346, 410, 431, 1022, 1301, 1318, 1375
HpyCH4III	ACNGT	2 cut(s)	280, 1464
HpyCH4IV	ACGT	3 cut(s)	933, 1377, 1594
HpyF10VI	GCNNNNNNNGC	6 cut(s)	68, 237, 263, 519, 748, 1589
HpyF3I	CTNAG	4 cut(s)	366, 378, 790, 1628
HpySE526I	ACGT	3 cut(s)	933, 1377, 1594
Hsp92I	GRCGYC	2 cut(s)	477, 1263
HspAI	GCGC	2 cut(s)	81, 257
Ksp22I	TGATCA	1 cut(s)	406
Kzo9I	GATC	8 cut(s)	18, 177, 298, 306, 406, 466, 675, 1624
LguI	GCTCTTC	3 cut(s)	117, 847, 963
LmnI	GCTCC	4 cut(s)	88, 789, 1048, 1056
Lsp1109I	GCAGC	3 cut(s)	80, 931, 1531
LweI	GCATC	5 cut(s)	643, 1351, 1431, 1438, 1566
MaeI	CTAG	5 cut(s)	47, 294, 780, 1106, 1518
MaeII	ACGT	3 cut(s)	933, 1377, 1594
MaeIII	GTNAC	2 cut(s)	247, 394
MalI	GATC	8 cut(s)	20, 179, 300, 308, 408, 468, 677, 1626
MboI	GATC	8 cut(s)	18, 177, 298, 306, 406, 466, 675, 1624
MfeI	CAATTG	1 cut(s)	1437
MhlI	GDGCHC	6 cut(s)	93, 233, 500, 515, 842, 1053
MlsI	TGGCCA	1 cut(s)	56
MluNI	TGGCCA	1 cut(s)	56
MlyI	GAGTC	4 cut(s)	185, 358, 370, 685
MmeI	TCCRAC	1 cut(s)	1360
Mox20I	TGGCCA	1 cut(s)	56
MroXI	GAANNNNTTC	1 cut(s)	420
MscI	TGGCCA	1 cut(s)	56
MseI	TTAA	3 cut(s)	453, 1142, 1347
MslI	CAYNNNNRTG	2 cut(s)	1199, 1562
Msp20I	TGGCCA	1 cut(s)	56
MspA1I	CMGCKG	1 cut(s)	820
MspI	CCGG	6 cut(s)	245, 399, 431, 926, 1046, 1229
MspR9I	CCNGG	5 cut(s)	245, 426, 926, 927, 1046
MunI	CAATTG	1 cut(s)	1437
Mva1269I	GAATGC	2 cut(s)	320, 1191
MvaI	CCWGG	1 cut(s)	426
MvnI	CGCG	1 cut(s)	1472
MwoI	GCNNNNNNNGC	6 cut(s)	68, 237, 263, 519, 748, 1589
NciI	CCSGG	4 cut(s)	245, 926, 927, 1046
NcoI	CCATGG	3 cut(s)	57, 829, 1110
NdeII	GATC	8 cut(s)	18, 177, 298, 306, 406, 466, 675, 1624
NlaIV	GGNNCC	4 cut(s)	231, 232, 497, 785
NmuCI	GTSAC	2 cut(s)	247, 394
NruI	TCGCGA	1 cut(s)	1472
NspI	RCATGY	3 cut(s)	43, 522, 1544
PagI	TCATGA	2 cut(s)	469, 732
PceI	AGGCCT	2 cut(s)	420, 953
PciSI	GCTCTTC	3 cut(s)	117, 847, 963
PcsI	WCGNNNNNNNCGW	1 cut(s)	186
PctI	GAATGC	2 cut(s)	320, 1191
PdmI	GAANNNNTTC	1 cut(s)	420
PfeI	GAWTC	2 cut(s)	755, 1396
PflFI	GACNNNGTC	2 cut(s)	367, 1168
PfoI	TCCNGGA	1 cut(s)	1044
PkrI	GCNGC	4 cut(s)	70, 164, 921, 1546
PleI	GAGTC	4 cut(s)	185, 358, 370, 685
PpsI	GAGTC	4 cut(s)	185, 358, 370, 685
Ppu21I	YACGTR	1 cut(s)	1595
PshAI	GACNNNNGTC	1 cut(s)	195
PshBI	ATTAAT	1 cut(s)	453
PsiI	TTATAA	1 cut(s)	1636
Psp124BI	GAGCTC	2 cut(s)	842, 1053
Psp6I	CCWGG	1 cut(s)	424
PspEI	GGTNACC	1 cut(s)	247
PspFI	CCCAGC	1 cut(s)	747
PspGI	CCWGG	1 cut(s)	424
PspN4I	GGNNCC	4 cut(s)	231, 232, 497, 785
PspOMI	GGGCCC	1 cut(s)	229
PspPI	GGNCC	3 cut(s)	229, 230, 428
PstI	CTGCAG	1 cut(s)	492
PsyI	GACNNNGTC	2 cut(s)	367, 1168
PvuII	CAGCTG	1 cut(s)	820
RruI	TCGCGA	1 cut(s)	1472
RsaI	GTAC	1 cut(s)	705
RsaNI	GTAC	1 cut(s)	704
RseI	CAYNNNNRTG	2 cut(s)	1199, 1562
SacI	GAGCTC	2 cut(s)	842, 1053
SapI	GCTCTTC	3 cut(s)	117, 847, 963
SaqAI	TTAA	3 cut(s)	453, 1142, 1347
SatI	GCNGC	4 cut(s)	69, 163, 920, 1545
Sau3AI	GATC	8 cut(s)	18, 177, 298, 306, 406, 466, 675, 1624
Sau96I	GGNCC	3 cut(s)	229, 230, 428
SchI	GAGTC	4 cut(s)	185, 358, 370, 685
ScrFI	CCNGG	5 cut(s)	245, 426, 926, 927, 1046
SduI	GDGCHC	6 cut(s)	93, 233, 500, 515, 842, 1053
SfaNI	GCATC	5 cut(s)	643, 1351, 1431, 1438, 1566
SfcI	CTRYAG	3 cut(s)	488, 501, 629
SinI	GGWCC	1 cut(s)	428
SmaI	CCCGGG	1 cut(s)	927
SmiMI	CAYNNNNRTG	2 cut(s)	1199, 1562
SmlI	CTYRAG	2 cut(s)	28, 657
SmoI	CTYRAG	2 cut(s)	28, 657
SseBI	AGGCCT	2 cut(s)	420, 953
SsiI	CCGC	4 cut(s)	66, 144, 162, 534
SspI	AATATT	1 cut(s)	1302
SspMI	CTAG	5 cut(s)	47, 294, 780, 1106, 1518
SstI	GAGCTC	2 cut(s)	842, 1053
StuI	AGGCCT	2 cut(s)	420, 953
StyD4I	CCNGG	5 cut(s)	243, 424, 924, 925, 1044
StyI	CCWWGG	6 cut(s)	57, 579, 829, 1074, 1110, 1155
TaaI	ACNGT	2 cut(s)	280, 1464
TaiI	ACGT	3 cut(s)	936, 1380, 1597
TaqI	TCGA	4 cut(s)	93, 180, 1425, 1432
TaqII	GACCGA	1 cut(s)	198
TatI	WGTACW	1 cut(s)	703
TauI	GCSGC	1 cut(s)	165
TfiI	GAWTC	2 cut(s)	755, 1396
Tru1I	TTAA	3 cut(s)	453, 1142, 1347
Tru9I	TTAA	3 cut(s)	453, 1142, 1347
TscAI	CASTG	3 cut(s)	93, 322, 497
TseFI	GTSAC	2 cut(s)	247, 394
TseI	GCWGC	3 cut(s)	68, 919, 1544
Tsp45I	GTSAC	2 cut(s)	247, 394
TspMI	CCCGGG	1 cut(s)	925
TspRI	CASTG	3 cut(s)	93, 322, 497
Tth111I	GACNNNGTC	2 cut(s)	367, 1168
VpaK11BI	GGWCC	1 cut(s)	428
VspI	ATTAAT	1 cut(s)	453
XapI	RAATTY	3 cut(s)	13, 870, 1503
XceI	RCATGY	3 cut(s)	43, 522, 1544
XmaI	CCCGGG	1 cut(s)	925
XmiI	GTMKAC	2 cut(s)	126, 480
XmnI	GAANNNNTTC	1 cut(s)	420
XspI	CTAG	5 cut(s)	47, 294, 780, 1106, 1518

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.