Rw5G018860

alpha-amylase subtilisin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003122
Physical Location & Seq
Forward (+)
24285132 .. 24285749
618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G018860.1

Sequence Viewer

Length: 618 bp
ATGTCCCTGAAGAACTTAATTGGAAGCCTGAGCATGATCGGCAGCATATTCATGGCCATGGCAACCATAAACCAAACATCTCCGGTGCTCGATACGTCTGGGAAGGCACTTAAATCCAGCATAGATTACTACATCAAACCCGTCAGTACTTACAAAGGTGGTGCTTTAACTCTAATAAACCGGGAGCCATATTTATGTCCACTCTACGTTGGCCAAGGAAATGTCTCAGGCTGGCCTTTTCAGTTCAAGCCATTTTTCGACGGAGAAACAGTGGTGAGGGAGAGTAGGGATCAAATGATTATATCTCGTGTCTCTACACATTGCAGGCGGTCAAGTTCATGGTCGATTGAAAAAGCTAACCCAGAGGACCAACGGAAATTAATTGCTATGCAAGAAACTGCAAGAGGAAAACTGCCAAATGGTAAATACTTTAGGATTACTAGATCACAGCCGGAGGTGGATGGTAATAGCTACATTCTGAGATGGTGTCCCACTGATCTGTGTTCTAATTGTACATTCGCTGCTTGTGGAAATATTGGTTCTTTAGATTCGAATGGAGGGAAGAGGTTGTTGGCTTTGAATGGTTCTGAGCTTCCGGTTGTGTTTGAAAGGGCATGA

Protein Analysis

205

Amino Acids

22.66

Weight (kDa)

9.18

Isoelectric Point (pI)

49.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 29 - 203 3.9e-33 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 328
AclWI GGATC 1 cut(s) 297
AcoI YGGCCR 2 cut(s) 54, 211
AcuI CTGAAG 1 cut(s) 29
AfaI GTAC 2 cut(s) 148, 514
AgsI TTSAA 4 cut(s) 247, 350, 580, 608
AjuI GAANNNNNNNTTGG 2 cut(s) 554, 586
AluBI AGCT 3 cut(s) 356, 471, 592
AluI AGCT 3 cut(s) 356, 471, 592
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 2 cut(s) 229, 316
AlwI GGATC 1 cut(s) 297
AoxI GGCC 3 cut(s) 54, 211, 233
ApeKI GCWGC 2 cut(s) 42, 521
AseI ATTAAT 1 cut(s) 380
AspS9I GGNCC 1 cut(s) 367
AsuC2I CCSGG 1 cut(s) 182
AsuHPI GGTGA 1 cut(s) 286
AsuII TTCGAA 1 cut(s) 551
AvaII GGWCC 1 cut(s) 367
BalI TGGCCA 2 cut(s) 56, 213
BauI CACGAG 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 2 cut(s) 54, 508
BccI CCATC 2 cut(s) 455, 477
BcnI CCSGG 1 cut(s) 182
BcoDI GTCTC 2 cut(s) 229, 316
BfaI CTAG 1 cut(s) 441
BisI GCNGC 2 cut(s) 43, 522
BlsI GCNGC 2 cut(s) 44, 523
BmcAI AGTACT 1 cut(s) 148
Bme1390I CCNGG 1 cut(s) 182
Bme18I GGWCC 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 367
BmiI GGNNCC 1 cut(s) 186
BmrFI CCNGG 1 cut(s) 182
Bpu10I CCTNAGC 1 cut(s) 29
Bpu14I TTCGAA 1 cut(s) 551
BpuMI CCSGG 1 cut(s) 182
BsaJI CCNNGG 2 cut(s) 57, 214
BsaWI WCCGGW 2 cut(s) 82, 595
BsaXI ACNNNNNCTCC 2 cut(s) 255, 285
Bse3DI GCAATG 1 cut(s) 319
BseDI CCNNGG 2 cut(s) 57, 214
BseGI GGATG 1 cut(s) 466
BseMI GCAATG 1 cut(s) 319
BseMII CTCAG 4 cut(s) 20, 240, 470, 579
BseXI GCAGC 2 cut(s) 54, 508
BshFI GGCC 3 cut(s) 56, 213, 235
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 4 cut(s) 83, 181, 452, 596
BslFI GGGAC 1 cut(s) 474
BsmAI GTCTC 2 cut(s) 229, 316
BsmFI GGGAC 1 cut(s) 474
BsnI GGCC 3 cut(s) 56, 213, 235
Bsp119I TTCGAA 1 cut(s) 551
Bsp1286I GDGCHC 1 cut(s) 90
Bsp1407I TGTACA 1 cut(s) 512
Bsp143I GATC 4 cut(s) 36, 289, 443, 496
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 1 cut(s) 328
BspANI GGCC 3 cut(s) 56, 213, 235
BspCNI CTCAG 4 cut(s) 21, 239, 471, 580
BspLI GGNNCC 1 cut(s) 186
BspPI GGATC 1 cut(s) 297
BspT104I TTCGAA 1 cut(s) 551
BsrDI GCAATG 1 cut(s) 319
BsrGI TGTACA 1 cut(s) 512
BssECI CCNNGG 2 cut(s) 57, 214
BssMI GATC 4 cut(s) 36, 289, 443, 496
BssSI CACGAG 1 cut(s) 306
BssT1I CCWWGG 2 cut(s) 57, 214
Bst2BI CACGAG 1 cut(s) 306
Bst4CI ACNGT 1 cut(s) 271
Bst6I CTCTTC 1 cut(s) 557
BstAUI TGTACA 1 cut(s) 512
BstBI TTCGAA 1 cut(s) 551
BstC8I GCNNGC 2 cut(s) 233, 326
BstDEI CTNAG 4 cut(s) 29, 226, 479, 588
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 1 cut(s) 466
BstKTI GATC 4 cut(s) 39, 292, 446, 499
BstMAI GTCTC 2 cut(s) 229, 316
BstMBI GATC 4 cut(s) 36, 289, 443, 496
BstMWI GCNNNNNNNGC 1 cut(s) 39
BstSCI CCNGG 1 cut(s) 180
BstV1I GCAGC 2 cut(s) 54, 508
BsuRI GGCC 3 cut(s) 56, 213, 235
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 1 cut(s) 466
BtsIMutI CAGTG 2 cut(s) 276, 492
Cac8I GCNNGC 2 cut(s) 233, 326
Cfr13I GGNCC 1 cut(s) 367
Csp6I GTAC 2 cut(s) 147, 513
CviAII CATG 5 cut(s) 34, 52, 58, 339, 615
CviQI GTAC 2 cut(s) 147, 513
DdeI CTNAG 4 cut(s) 29, 226, 479, 588
DpnI GATC 4 cut(s) 38, 291, 445, 498
DpnII GATC 4 cut(s) 36, 289, 443, 496
EaeI YGGCCR 2 cut(s) 54, 211
Eam1104I CTCTTC 1 cut(s) 557
EarI CTCTTC 1 cut(s) 557
Eco130I CCWWGG 2 cut(s) 57, 214
Eco47I GGWCC 1 cut(s) 367
Eco57I CTGAAG 1 cut(s) 29
EcoT14I CCWWGG 2 cut(s) 57, 214
ErhI CCWWGG 2 cut(s) 57, 214
FaeI CATG 5 cut(s) 37, 55, 61, 342, 618
FaqI GGGAC 1 cut(s) 474
FatI CATG 5 cut(s) 33, 51, 57, 338, 614
Fnu4HI GCNGC 2 cut(s) 43, 522
FokI GGATG 1 cut(s) 473
Fsp4HI GCNGC 2 cut(s) 43, 522
FspBI CTAG 1 cut(s) 441
GluI GCNGC 2 cut(s) 43, 522
HaeIII GGCC 3 cut(s) 56, 213, 235
HapII CCGG 4 cut(s) 83, 181, 452, 596
Hin1II CATG 5 cut(s) 37, 55, 61, 342, 618
HinfI GANTC 1 cut(s) 548
HpaII CCGG 4 cut(s) 83, 181, 452, 596
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 1 cut(s) 200
Hpy188I TCNGA 2 cut(s) 480, 589
Hpy8I GTNNAC 1 cut(s) 200
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 1 cut(s) 97
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4IV ACGT 2 cut(s) 95, 207
HpyCH4V TGCA 3 cut(s) 324, 391, 401
HpyF10VI GCNNNNNNNGC 1 cut(s) 39
HpyF3I CTNAG 4 cut(s) 29, 226, 479, 588
HpySE526I ACGT 2 cut(s) 95, 207
Hsp92II CATG 5 cut(s) 37, 55, 61, 342, 618
Kzo9I GATC 4 cut(s) 36, 289, 443, 496
LmnI GCTCC 1 cut(s) 184
Lsp1109I GCAGC 2 cut(s) 54, 508
MaeI CTAG 1 cut(s) 441
MaeII ACGT 2 cut(s) 95, 207
MalI GATC 4 cut(s) 38, 291, 445, 498
MboI GATC 4 cut(s) 36, 289, 443, 496
MboII GAAGA 2 cut(s) 22, 574
MhlI GDGCHC 1 cut(s) 90
MlsI TGGCCA 2 cut(s) 56, 213
MluCI AATT 4 cut(s) 18, 377, 381, 508
MluNI TGGCCA 2 cut(s) 56, 213
MnlI CCTC 6 cut(s) 270, 358, 398, 448, 551, 558
Mox20I TGGCCA 2 cut(s) 56, 213
MscI TGGCCA 2 cut(s) 56, 213
MseI TTAA 4 cut(s) 17, 111, 167, 380
MslI CAYNNNNRTG 3 cut(s) 50, 56, 193
Msp20I TGGCCA 2 cut(s) 56, 213
MspI CCGG 4 cut(s) 83, 181, 452, 596
MspR9I CCNGG 1 cut(s) 182
MwoI GCNNNNNNNGC 1 cut(s) 39
NciI CCSGG 1 cut(s) 182
NcoI CCATGG 1 cut(s) 57
NdeII GATC 4 cut(s) 36, 289, 443, 496
NlaIII CATG 5 cut(s) 37, 55, 61, 342, 618
NlaIV GGNNCC 1 cut(s) 186
NspV TTCGAA 1 cut(s) 551
PfeI GAWTC 1 cut(s) 548
PkrI GCNGC 2 cut(s) 44, 523
PshBI ATTAAT 1 cut(s) 380
PspN4I GGNNCC 1 cut(s) 186
PspPI GGNCC 1 cut(s) 367
RsaI GTAC 2 cut(s) 148, 514
RsaNI GTAC 2 cut(s) 147, 513
RseI CAYNNNNRTG 3 cut(s) 50, 56, 193
SaqAI TTAA 4 cut(s) 17, 111, 167, 380
SatI GCNGC 2 cut(s) 43, 522
Sau3AI GATC 4 cut(s) 36, 289, 443, 496
Sau96I GGNCC 1 cut(s) 367
ScaI AGTACT 1 cut(s) 148
ScrFI CCNGG 1 cut(s) 182
SduI GDGCHC 1 cut(s) 90
SetI ASST 8 cut(s) 98, 160, 210, 358, 459, 473, 569, 594
SfuI TTCGAA 1 cut(s) 551
SinI GGWCC 1 cut(s) 367
SmiMI CAYNNNNRTG 3 cut(s) 50, 56, 193
Sse9I AATT 4 cut(s) 18, 377, 381, 508
SsiI CCGC 1 cut(s) 328
SspI AATATT 1 cut(s) 535
SspMI CTAG 1 cut(s) 441
StyD4I CCNGG 1 cut(s) 180
StyI CCWWGG 2 cut(s) 57, 214
TaaI ACNGT 1 cut(s) 271
TaiI ACGT 2 cut(s) 98, 210
TaqI TCGA 4 cut(s) 90, 258, 344, 551
TasI AATT 4 cut(s) 18, 377, 381, 508
TatI WGTACW 2 cut(s) 146, 512
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 4 cut(s) 17, 111, 167, 380
Tru9I TTAA 4 cut(s) 17, 111, 167, 380
TscAI CASTG 2 cut(s) 276, 499
TseI GCWGC 2 cut(s) 42, 521
TspDTI ATGAA 2 cut(s) 40, 327
TspGWI ACGGA 2 cut(s) 276, 388
TspRI CASTG 2 cut(s) 276, 499
VpaK11BI GGWCC 1 cut(s) 367
VspI ATTAAT 1 cut(s) 380
XspI CTAG 1 cut(s) 441
ZrmI AGTACT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.