Rorug05G0112300

alpha-amylase subtilisin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003122
Physical Location & Seq
Reverse (-)
9998786 .. 10000720
1935 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0112300.1

Sequence Viewer

Length: 726 bp
ATGCAAACTAAGCAGGGCAACCATTCTATAGCCTGGAAAGGTATTCTTGAAAATAGAGATCTTATAATCCAGGGTATGCGTTGGATTGTTGGTAATGGTACTGACATCAATTTCTGGACTTTCAATTGGGCATTTTCTTTTCCTTTGATCAATCTTATTTCGACTACTAATAGAAATTCTATTAATATTGATGAGAATGTGAGTGATTATATTGATAATGGTTGTTGGAACGCTAATAAACTTTCTGTTTTTCTTGATCATGATACTGTTAACTCTATTTTGAGTATTCCCCTTCCTGCTTTGAACTCTAAGGATGAATTTGTTTGGGGTCCAACCTCTAGTGGTGTTTTTTCTGTTAAATCTGCCACTTGGATTCAATGTGACTCTGTTGCTCCTCATTCGAGGGTTAAAATTTTGAATGAAATCTGGAGACTTGACATTCCTCCGAAGGTCAAGATTTTCTCTTGGCTCTTAGCTAGGGGGAGATTGAAAACTAGGGCAAGACTTGCTCATTATTCTACTAATTTTGACTCTTTGTGTGCCCTTTGCAGCTCAGGGGATGAGGACATAAACCACCTTTTCTTCTCCTGCCCCTTTGCTGCAAATGTTTGGCGCTCTGCTGGTATTATCAATTCAGATTTCCAAAACTTTGAAGATTGGTTTTTTTCTTGGTTCAGGAAGGATTACCAAAAGTCTATTACTGAAAATCTTCTCCTTCTTTGTTGA

Protein Analysis

241

Amino Acids

27.65

Weight (kDa)

6.51

Isoelectric Point (pI)

25.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 117 - 204 2.7e-21 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 65
AcsI RAATTY 3 cut(s) 175, 317, 411
AfaI GTAC 1 cut(s) 100
AgsI TTSAA 7 cut(s) 50, 124, 304, 377, 418, 490, 653
AjnI CCWGG 2 cut(s) 32, 69
AluBI AGCT 2 cut(s) 476, 552
AluI AGCT 2 cut(s) 476, 552
Alw26I GTCTC 1 cut(s) 424
ApeKI GCWGC 2 cut(s) 549, 599
ApoI RAATTY 3 cut(s) 175, 317, 411
AseI ATTAAT 1 cut(s) 183
Asp700I GAANNNNTTC 1 cut(s) 708
AspLEI GCGC 1 cut(s) 615
AspS9I GGNCC 1 cut(s) 329
AvaII GGWCC 1 cut(s) 329
BaeGI GKGCMC 1 cut(s) 544
BbvI GCAGC 2 cut(s) 561, 586
BciT130I CCWGG 2 cut(s) 34, 71
BclI TGATCA 2 cut(s) 147, 256
BcoDI GTCTC 1 cut(s) 424
BfaI CTAG 3 cut(s) 339, 477, 495
BfmI CTRYAG 1 cut(s) 27
BfoI RGCGCY 1 cut(s) 616
BglII AGATCT 1 cut(s) 58
BisI GCNGC 2 cut(s) 550, 600
BlsI GCNGC 2 cut(s) 551, 601
Bme1390I CCNGG 2 cut(s) 34, 71
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 329
BmiI GGNNCC 1 cut(s) 330
BmrFI CCNGG 2 cut(s) 34, 71
BpmI CTGGAG 1 cut(s) 448
Bpu10I CCTNAGC 1 cut(s) 553
BsaJI CCNNGG 1 cut(s) 70
BseBI CCWGG 2 cut(s) 34, 71
BseDI CCNNGG 1 cut(s) 70
BseGI GGATG 2 cut(s) 319, 565
BseMII CTCAG 1 cut(s) 567
BseRI GAGGAG 1 cut(s) 384
BseSI GKGCMC 1 cut(s) 544
BseXI GCAGC 2 cut(s) 561, 586
BsmAI GTCTC 1 cut(s) 424
Bsp1286I GDGCHC 1 cut(s) 544
Bsp143I GATC 3 cut(s) 58, 147, 256
BspCNI CTCAG 1 cut(s) 566
BspHI TCATGA 1 cut(s) 259
BspLI GGNNCC 1 cut(s) 330
BssECI CCNNGG 1 cut(s) 70
BssMI GATC 3 cut(s) 58, 147, 256
Bst2UI CCWGG 2 cut(s) 34, 71
Bst4CI ACNGT 1 cut(s) 268
BstAPI GCANNNNNTGC 1 cut(s) 506
BstDEI CTNAG 4 cut(s) 9, 309, 472, 553
BstF5I GGATG 2 cut(s) 319, 565
BstH2I RGCGCY 1 cut(s) 616
BstHHI GCGC 1 cut(s) 615
BstKTI GATC 3 cut(s) 61, 150, 259
BstMAI GTCTC 1 cut(s) 424
BstMBI GATC 3 cut(s) 58, 147, 256
BstMWI GCNNNNNNNGC 2 cut(s) 10, 506
BstNI CCWGG 2 cut(s) 34, 71
BstSCI CCNGG 2 cut(s) 32, 69
BstSFI CTRYAG 1 cut(s) 27
BstSLI GKGCMC 1 cut(s) 544
BstV1I GCAGC 2 cut(s) 561, 586
BstX2I RGATCY 1 cut(s) 58
BstYI RGATCY 1 cut(s) 58
BtsCI GGATG 2 cut(s) 319, 565
CciI TCATGA 1 cut(s) 259
CfoI GCGC 1 cut(s) 615
Cfr13I GGNCC 1 cut(s) 329
Csp6I GTAC 1 cut(s) 99
CviAII CATG 1 cut(s) 260
CviJI RGCY 4 cut(s) 32, 469, 476, 552
CviKI_1 RGCY 4 cut(s) 32, 469, 476, 552
CviQI GTAC 1 cut(s) 99
DdeI CTNAG 4 cut(s) 9, 309, 472, 553
DpnI GATC 3 cut(s) 60, 149, 258
DpnII GATC 3 cut(s) 58, 147, 256
Eco47I GGWCC 1 cut(s) 329
EcoRII CCWGG 2 cut(s) 32, 69
FaeI CATG 1 cut(s) 263
FaiI YATR 6 cut(s) 29, 65, 77, 210, 261, 569
FalI AAGNNNNNCTT 2 cut(s) 30, 62
FatI CATG 1 cut(s) 259
FbaI TGATCA 2 cut(s) 147, 256
Fnu4HI GCNGC 2 cut(s) 550, 600
FokI GGATG 2 cut(s) 326, 572
Fsp4HI GCNGC 2 cut(s) 550, 600
FspBI CTAG 3 cut(s) 339, 477, 495
GlaI GCGC 1 cut(s) 614
GluI GCNGC 2 cut(s) 550, 600
GsuI CTGGAG 1 cut(s) 448
HaeII RGCGCY 1 cut(s) 616
HhaI GCGC 1 cut(s) 615
Hin1II CATG 1 cut(s) 263
Hin6I GCGC 1 cut(s) 613
HinP1I GCGC 1 cut(s) 613
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 3 cut(s) 373, 383, 530
HpaI GTTAAC 1 cut(s) 271
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 2 cut(s) 447, 637
Hpy188III TCNNGA 7 cut(s) 47, 115, 254, 260, 427, 454, 676
Hpy8I GTNNAC 1 cut(s) 271
HpyAV CCTTC 4 cut(s) 302, 442, 673, 725
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4V TGCA 3 cut(s) 4, 549, 602
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 506
HpyF3I CTNAG 4 cut(s) 9, 309, 472, 553
Hsp92II CATG 1 cut(s) 263
HspAI GCGC 1 cut(s) 613
Ksp22I TGATCA 2 cut(s) 147, 256
KspAI GTTAAC 1 cut(s) 271
Kzo9I GATC 3 cut(s) 58, 147, 256
LmnI GCTCC 1 cut(s) 397
Lsp1109I GCAGC 2 cut(s) 561, 586
MaeI CTAG 3 cut(s) 339, 477, 495
MaeIII GTNAC 1 cut(s) 380
MalI GATC 3 cut(s) 60, 149, 258
MboI GATC 3 cut(s) 58, 147, 256
MboII GAAGA 3 cut(s) 574, 665, 701
MfeI CAATTG 1 cut(s) 124
MflI RGATCY 1 cut(s) 58
MhlI GDGCHC 1 cut(s) 544
MluCI AATT 7 cut(s) 109, 124, 175, 317, 411, 523, 631
MlyI GAGTC 2 cut(s) 377, 524
MmeI TCCRAC 3 cut(s) 62, 206, 356
MnlI CCTC 5 cut(s) 346, 396, 405, 453, 556
MroXI GAANNNNTTC 1 cut(s) 708
MseI TTAA 4 cut(s) 183, 270, 357, 408
MspR9I CCNGG 2 cut(s) 34, 71
MunI CAATTG 1 cut(s) 124
MvaI CCWGG 2 cut(s) 34, 71
MwoI GCNNNNNNNGC 2 cut(s) 10, 506
NdeII GATC 3 cut(s) 58, 147, 256
NlaIII CATG 1 cut(s) 263
NlaIV GGNNCC 1 cut(s) 330
NmuCI GTSAC 1 cut(s) 380
PagI TCATGA 1 cut(s) 259
PdmI GAANNNNTTC 1 cut(s) 708
PfeI GAWTC 1 cut(s) 373
PkrI GCNGC 2 cut(s) 551, 601
PleI GAGTC 2 cut(s) 377, 524
PpsI GAGTC 2 cut(s) 377, 524
PshBI ATTAAT 1 cut(s) 183
PsiI TTATAA 1 cut(s) 65
Psp6I CCWGG 2 cut(s) 32, 69
PspGI CCWGG 2 cut(s) 32, 69
PspN4I GGNNCC 1 cut(s) 330
PspPI GGNCC 1 cut(s) 329
PsuI RGATCY 1 cut(s) 58
RsaI GTAC 1 cut(s) 100
RsaNI GTAC 1 cut(s) 99
SaqAI TTAA 4 cut(s) 183, 270, 357, 408
SatI GCNGC 2 cut(s) 550, 600
Sau3AI GATC 3 cut(s) 58, 147, 256
Sau96I GGNCC 1 cut(s) 329
SchI GAGTC 2 cut(s) 377, 524
ScrFI CCNGG 2 cut(s) 34, 71
SduI GDGCHC 1 cut(s) 544
SetI ASST 6 cut(s) 43, 338, 453, 478, 554, 579
SfcI CTRYAG 1 cut(s) 27
SinI GGWCC 1 cut(s) 329
Sse9I AATT 7 cut(s) 109, 124, 175, 317, 411, 523, 631
SspI AATATT 1 cut(s) 187
SspMI CTAG 3 cut(s) 339, 477, 495
StyD4I CCNGG 2 cut(s) 32, 69
TaaI ACNGT 1 cut(s) 268
TaqI TCGA 2 cut(s) 161, 401
TasI AATT 7 cut(s) 109, 124, 175, 317, 411, 523, 631
TfiI GAWTC 1 cut(s) 373
Tru1I TTAA 4 cut(s) 183, 270, 357, 408
Tru9I TTAA 4 cut(s) 183, 270, 357, 408
TseFI GTSAC 1 cut(s) 380
TseI GCWGC 2 cut(s) 549, 599
Tsp45I GTSAC 1 cut(s) 380
TspDTI ATGAA 2 cut(s) 330, 435
VpaK11BI GGWCC 1 cut(s) 329
VspI ATTAAT 1 cut(s) 183
XapI RAATTY 3 cut(s) 175, 317, 411
XmnI GAANNNNTTC 1 cut(s) 708
XspI CTAG 3 cut(s) 339, 477, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.