Rh5BG202800

alpha-amylase subtilisin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003122
Physical Location & Seq
Reverse (-)
22916425 .. 22918041
1617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG202800.1

Sequence Viewer

Length: 621 bp
ATGAAGTCCATGAATTTGATCGGAAGCTTGAGCTGTTGCATGTGGCTAGTGATGGCCATGGCAACCGCAGCCCAAGATAGCGCTCCAGTGCTCGACACCTCTGGGCAGGCTCTTCAATCCGGTGTAGACTACTACATCAAACCCGCCATCACCGACAACGGCGGCCGTTTCACTTTGATCGACCGAAACGACTCGTGTCCTTTCTATGCTGGTCAAGAAAACACCTCGGGCCCCGAGGGCTTCCCGGTGACCTTTGCGCCTTTCGCAGAGGGCGAGACGGTGGTGAGGGAGACTAGGGATCAGAAGATCACATTCTCTGCATTCACAATCTGTGTGCAGTCAACGGCATGGAAGGTCGGTGAGACTCAGTCAGAGACTCAGAGGAGATTGATTGTCACCGGAATTGATCAAAACGAAGGCCTTCCAGGTCCGGCCAGAAACTACTTCAGGATTAATAAACAGGCAGATCATGATGGCGTCTACAACCTGCAGTGGTGCCCTACAGAAGTGTGCCCGACATGCAGGTTCATTTGCGGAGATGTGGGTGCTTTGGTTGAGAATGACAAGAGGCTGTTGGCCTTGGATGGTTCTGTGCTTCCTGTTGTCTTCGAGAAGGCTTAA

Protein Analysis

206

Amino Acids

22.51

Weight (kDa)

4.73

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 30 - 205 6e-48 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 495, 513
AccB1I GGYRCC 1 cut(s) 495
AccI GTMKAC 2 cut(s) 126, 480
AciI CCGC 4 cut(s) 66, 144, 162, 534
AclWI GGATC 1 cut(s) 306
AcoI YGGCCR 3 cut(s) 54, 163, 432
AcsI RAATTY 1 cut(s) 13
AcuI CTGAAG 1 cut(s) 430
AcyI GRCGYC 1 cut(s) 477
AfeI AGCGCT 1 cut(s) 82
AgsI TTSAA 1 cut(s) 116
AjnI CCWGG 1 cut(s) 424
AluBI AGCT 2 cut(s) 27, 33
AluI AGCT 2 cut(s) 27, 33
Alw21I GWGCWC 1 cut(s) 93
Alw26I GTCTC 4 cut(s) 269, 284, 356, 368
AlwI GGATC 1 cut(s) 306
Ama87I CYCGRG 2 cut(s) 226, 233
Aor51HI AGCGCT 1 cut(s) 82
AoxI GGCC 6 cut(s) 54, 163, 229, 418, 432, 576
ApaI GGGCCC 1 cut(s) 233
ApeKI GCWGC 1 cut(s) 68
ApoI RAATTY 1 cut(s) 13
AseI ATTAAT 1 cut(s) 453
Asp700I GAANNNNTTC 1 cut(s) 420
AspLEI GCGC 2 cut(s) 83, 259
AspS9I GGNCC 3 cut(s) 229, 230, 428
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 5 cut(s) 142, 259, 295, 371, 388
AvaI CYCGRG 2 cut(s) 226, 233
AvaII GGWCC 1 cut(s) 428
BaeGI GKGCMC 3 cut(s) 233, 500, 515
BalI TGGCCA 1 cut(s) 56
BanI GGYRCC 1 cut(s) 495
BanII GRGCYC 1 cut(s) 233
BauI CACGAG 1 cut(s) 193
BbsI GAAGAC 1 cut(s) 598
Bbv12I GWGCWC 1 cut(s) 93
BbvI GCAGC 1 cut(s) 80
BccI CCATC 4 cut(s) 46, 155, 467, 578
BceAI ACGGC 3 cut(s) 150, 175, 360
BciT130I CCWGG 1 cut(s) 426
BclI TGATCA 1 cut(s) 406
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 4 cut(s) 269, 284, 356, 368
BfaI CTAG 2 cut(s) 47, 294
BfmI CTRYAG 2 cut(s) 488, 501
BfoI RGCGCY 1 cut(s) 84
BfuAI ACCTGC 2 cut(s) 495, 513
BglI GCCNNNNNGGC 1 cut(s) 237
BisI GCNGC 2 cut(s) 69, 163
BlsI GCNGC 2 cut(s) 70, 164
Bme1390I CCNGG 2 cut(s) 245, 426
Bme18I GGWCC 1 cut(s) 428
BmeT110I CYCGRG 2 cut(s) 226, 233
BmgT120I GGNCC 3 cut(s) 229, 230, 428
BmiI GGNNCC 3 cut(s) 231, 232, 497
BmrFI CCNGG 2 cut(s) 245, 426
BoxI GACNNNNGTC 1 cut(s) 195
BpiI GAAGAC 1 cut(s) 598
BpmI CTGGAG 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 49
BpuMI CCSGG 1 cut(s) 245
BsaHI GRCGYC 1 cut(s) 477
BsaJI CCNNGG 4 cut(s) 57, 225, 234, 579
BsaWI WCCGGW 2 cut(s) 119, 398
Bse1I ACTGG 1 cut(s) 86
BseBI CCWGG 1 cut(s) 426
BseDI CCNNGG 4 cut(s) 57, 225, 234, 579
BseGI GGATG 1 cut(s) 589
BseMII CTCAG 2 cut(s) 380, 392
BseNI ACTGG 1 cut(s) 86
BseRI GAGGAG 1 cut(s) 397
BseSI GKGCMC 3 cut(s) 233, 500, 515
BseX3I CGGCCG 1 cut(s) 163
BseXI GCAGC 1 cut(s) 80
BsgI GTGCAG 1 cut(s) 356
Bsh1285I CGRYCG 2 cut(s) 166, 184
BshFI GGCC 6 cut(s) 56, 165, 231, 420, 434, 578
BshNI GGYRCC 1 cut(s) 495
BsiEI CGRYCG 2 cut(s) 166, 184
BsiHKAI GWGCWC 1 cut(s) 93
BsiHKCI CYCGRG 2 cut(s) 226, 233
BsiSI CCGG 4 cut(s) 120, 245, 399, 431
BsmAI GTCTC 4 cut(s) 269, 284, 356, 368
BsmBI CGTCTC 1 cut(s) 269
BsmI GAATGC 1 cut(s) 320
BsnI GGCC 6 cut(s) 56, 165, 231, 420, 434, 578
BsoBI CYCGRG 2 cut(s) 226, 233
Bsp120I GGGCCC 1 cut(s) 229
Bsp1286I GDGCHC 4 cut(s) 93, 233, 500, 515
Bsp143I GATC 6 cut(s) 18, 177, 298, 306, 406, 466
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 4 cut(s) 66, 144, 162, 534
BspANI GGCC 6 cut(s) 56, 165, 231, 420, 434, 578
BspCNI CTCAG 2 cut(s) 379, 391
BspHI TCATGA 1 cut(s) 469
BspLI GGNNCC 3 cut(s) 231, 232, 497
BspMAI CTGCAG 1 cut(s) 492
BspMI ACCTGC 2 cut(s) 495, 513
BspPI GGATC 1 cut(s) 306
BspQI GCTCTTC 1 cut(s) 117
BspT107I GGYRCC 1 cut(s) 495
BsrI ACTGG 1 cut(s) 86
BssECI CCNNGG 4 cut(s) 57, 225, 234, 579
BssMI GATC 6 cut(s) 18, 177, 298, 306, 406, 466
BssNI GRCGYC 1 cut(s) 477
BssSI CACGAG 1 cut(s) 193
BssT1I CCWWGG 2 cut(s) 57, 579
Bst2BI CACGAG 1 cut(s) 193
Bst2UI CCWGG 1 cut(s) 426
Bst4CI ACNGT 1 cut(s) 280
Bst6I CTCTTC 1 cut(s) 117
BstACI GRCGYC 1 cut(s) 477
BstC8I GCNNGC 1 cut(s) 108
BstDEI CTNAG 2 cut(s) 366, 378
BstDSI CCRYGG 1 cut(s) 57
BstEII GGTNACC 1 cut(s) 247
BstF5I GGATG 1 cut(s) 589
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 2 cut(s) 83, 259
BstKTI GATC 6 cut(s) 21, 180, 301, 309, 409, 469
BstMAI GTCTC 4 cut(s) 269, 284, 356, 368
BstMBI GATC 6 cut(s) 18, 177, 298, 306, 406, 466
BstMCI CGRYCG 2 cut(s) 166, 184
BstMWI GCNNNNNNNGC 4 cut(s) 68, 237, 263, 519
BstNI CCWGG 1 cut(s) 426
BstNSI RCATGY 2 cut(s) 43, 522
BstPAI GACNNNNGTC 1 cut(s) 195
BstPI GGTNACC 1 cut(s) 247
BstSCI CCNGG 2 cut(s) 243, 424
BstSFI CTRYAG 2 cut(s) 488, 501
BstSLI GKGCMC 3 cut(s) 233, 500, 515
BstV1I GCAGC 1 cut(s) 80
BstV2I GAAGAC 1 cut(s) 598
BstZI CGGCCG 1 cut(s) 163
BsuRI GGCC 6 cut(s) 56, 165, 231, 420, 434, 578
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 1 cut(s) 589
BtsI GCAGTG 1 cut(s) 497
BtsIMutI CAGTG 2 cut(s) 93, 497
BveI ACCTGC 2 cut(s) 495, 513
Cac8I GCNNGC 1 cut(s) 108
CciI TCATGA 1 cut(s) 469
CfoI GCGC 2 cut(s) 83, 259
Cfr13I GGNCC 3 cut(s) 229, 230, 428
CseI GACGC 1 cut(s) 466
CviAII CATG 6 cut(s) 10, 40, 58, 348, 470, 519
DdeI CTNAG 2 cut(s) 366, 378
DpnI GATC 6 cut(s) 20, 179, 300, 308, 408, 468
DpnII GATC 6 cut(s) 18, 177, 298, 306, 406, 466
EaeI YGGCCR 3 cut(s) 54, 163, 432
EagI CGGCCG 1 cut(s) 163
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
EclXI CGGCCG 1 cut(s) 163
Eco130I CCWWGG 2 cut(s) 57, 579
Eco147I AGGCCT 1 cut(s) 420
Eco24I GRGCYC 1 cut(s) 233
Eco47I GGWCC 1 cut(s) 428
Eco47III AGCGCT 1 cut(s) 82
Eco52I CGGCCG 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 430
Eco88I CYCGRG 2 cut(s) 226, 233
Eco91I GGTNACC 1 cut(s) 247
EcoO109I RGGNCCY 1 cut(s) 230
EcoO65I GGTNACC 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 424
EcoT14I CCWWGG 2 cut(s) 57, 579
EcoT38I GRGCYC 1 cut(s) 233
ErhI CCWWGG 2 cut(s) 57, 579
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 6 cut(s) 13, 43, 61, 351, 473, 522
FaiI YATR 7 cut(s) 11, 41, 59, 207, 349, 471, 520
FatI CATG 6 cut(s) 9, 39, 57, 347, 469, 518
FauI CCCGC 1 cut(s) 151
FbaI TGATCA 1 cut(s) 406
FblI GTMKAC 2 cut(s) 126, 480
Fnu4HI GCNGC 2 cut(s) 69, 163
FokI GGATG 1 cut(s) 596
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 2 cut(s) 69, 163
FspBI CTAG 2 cut(s) 47, 294
GlaI GCGC 2 cut(s) 82, 258
GluI GCNGC 2 cut(s) 69, 163
GsuI CTGGAG 1 cut(s) 69
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 6 cut(s) 56, 165, 231, 420, 434, 578
HapII CCGG 4 cut(s) 120, 245, 399, 431
HgaI GACGC 1 cut(s) 466
HhaI GCGC 2 cut(s) 83, 259
Hin1I GRCGYC 1 cut(s) 477
Hin1II CATG 6 cut(s) 13, 43, 61, 351, 473, 522
Hin6I GCGC 2 cut(s) 81, 257
HinP1I GCGC 2 cut(s) 81, 257
HincII GTYRAC 1 cut(s) 342
HindII GTYRAC 1 cut(s) 342
HindIII AAGCTT 1 cut(s) 25
HinfI GANTC 3 cut(s) 191, 364, 376
HpaII CCGG 4 cut(s) 120, 245, 399, 431
HphI GGTGA 5 cut(s) 142, 259, 295, 371, 388
Hpy166II GTNNAC 3 cut(s) 127, 342, 481
Hpy188I TCNGA 4 cut(s) 23, 303, 373, 381
Hpy188III TCNNGA 4 cut(s) 215, 448, 470, 610
Hpy8I GTNNAC 3 cut(s) 127, 342, 481
HpyAV CCTTC 4 cut(s) 346, 410, 431, 607
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4V TGCA 5 cut(s) 39, 320, 337, 490, 522
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 237, 263, 519
HpyF3I CTNAG 2 cut(s) 366, 378
Hsp92I GRCGYC 1 cut(s) 477
Hsp92II CATG 6 cut(s) 13, 43, 61, 351, 473, 522
HspAI GCGC 2 cut(s) 81, 257
Ksp22I TGATCA 1 cut(s) 406
Kzo9I GATC 6 cut(s) 18, 177, 298, 306, 406, 466
LguI GCTCTTC 1 cut(s) 117
LmnI GCTCC 1 cut(s) 88
Lsp1109I GCAGC 1 cut(s) 80
MaeI CTAG 2 cut(s) 47, 294
MaeIII GTNAC 2 cut(s) 247, 394
MalI GATC 6 cut(s) 20, 179, 300, 308, 408, 468
MboI GATC 6 cut(s) 18, 177, 298, 306, 406, 466
MboII GAAGA 3 cut(s) 104, 316, 598
MhlI GDGCHC 4 cut(s) 93, 233, 500, 515
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 2 cut(s) 13, 402
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 3 cut(s) 185, 358, 370
MnlI CCTC 7 cut(s) 109, 229, 235, 262, 279, 375, 561
Mox20I TGGCCA 1 cut(s) 56
MroXI GAANNNNTTC 1 cut(s) 420
MscI TGGCCA 1 cut(s) 56
MseI TTAA 2 cut(s) 453, 619
Msp20I TGGCCA 1 cut(s) 56
MspI CCGG 4 cut(s) 120, 245, 399, 431
MspR9I CCNGG 2 cut(s) 245, 426
Mva1269I GAATGC 1 cut(s) 320
MvaI CCWGG 1 cut(s) 426
MwoI GCNNNNNNNGC 4 cut(s) 68, 237, 263, 519
NciI CCSGG 1 cut(s) 245
NcoI CCATGG 1 cut(s) 57
NdeII GATC 6 cut(s) 18, 177, 298, 306, 406, 466
NlaIII CATG 6 cut(s) 13, 43, 61, 351, 473, 522
NlaIV GGNNCC 3 cut(s) 231, 232, 497
NmuCI GTSAC 2 cut(s) 247, 394
NspI RCATGY 2 cut(s) 43, 522
PagI TCATGA 1 cut(s) 469
PceI AGGCCT 1 cut(s) 420
PciSI GCTCTTC 1 cut(s) 117
PcsI WCGNNNNNNNCGW 2 cut(s) 186, 270
PctI GAATGC 1 cut(s) 320
PdmI GAANNNNTTC 1 cut(s) 420
PflFI GACNNNGTC 1 cut(s) 367
PkrI GCNGC 2 cut(s) 70, 164
PleI GAGTC 3 cut(s) 185, 358, 370
PpsI GAGTC 3 cut(s) 185, 358, 370
PshAI GACNNNNGTC 1 cut(s) 195
PshBI ATTAAT 1 cut(s) 453
Psp6I CCWGG 1 cut(s) 424
PspEI GGTNACC 1 cut(s) 247
PspGI CCWGG 1 cut(s) 424
PspN4I GGNNCC 3 cut(s) 231, 232, 497
PspOMI GGGCCC 1 cut(s) 229
PspPI GGNCC 3 cut(s) 229, 230, 428
PstI CTGCAG 1 cut(s) 492
PsyI GACNNNGTC 1 cut(s) 367
SapI GCTCTTC 1 cut(s) 117
SaqAI TTAA 2 cut(s) 453, 619
SatI GCNGC 2 cut(s) 69, 163
Sau3AI GATC 6 cut(s) 18, 177, 298, 306, 406, 466
Sau96I GGNCC 3 cut(s) 229, 230, 428
SchI GAGTC 3 cut(s) 185, 358, 370
ScrFI CCNGG 2 cut(s) 245, 426
SduI GDGCHC 4 cut(s) 93, 233, 500, 515
SetI ASST 9 cut(s) 29, 35, 101, 227, 254, 357, 430, 489, 527
SfcI CTRYAG 2 cut(s) 488, 501
SinI GGWCC 1 cut(s) 428
SmlI CTYRAG 1 cut(s) 28
SmoI CTYRAG 1 cut(s) 28
Sse9I AATT 2 cut(s) 13, 402
SseBI AGGCCT 1 cut(s) 420
SsiI CCGC 4 cut(s) 66, 144, 162, 534
SspMI CTAG 2 cut(s) 47, 294
StuI AGGCCT 1 cut(s) 420
StyD4I CCNGG 2 cut(s) 243, 424
StyI CCWWGG 2 cut(s) 57, 579
TaaI ACNGT 1 cut(s) 280
TaqI TCGA 3 cut(s) 93, 180, 609
TaqII GACCGA 1 cut(s) 198
TasI AATT 2 cut(s) 13, 402
TauI GCSGC 1 cut(s) 165
Tru1I TTAA 2 cut(s) 453, 619
Tru9I TTAA 2 cut(s) 453, 619
TscAI CASTG 2 cut(s) 93, 497
TseFI GTSAC 2 cut(s) 247, 394
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 2 cut(s) 247, 394
TspDTI ATGAA 3 cut(s) 17, 26, 517
TspRI CASTG 2 cut(s) 93, 497
Tth111I GACNNNGTC 1 cut(s) 367
VpaK11BI GGWCC 1 cut(s) 428
VspI ATTAAT 1 cut(s) 453
XapI RAATTY 1 cut(s) 13
XceI RCATGY 2 cut(s) 43, 522
XmiI GTMKAC 2 cut(s) 126, 480
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 2 cut(s) 47, 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.