pycom03g19890

alpha-amylase subtilisin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003122
Physical Location & Seq
Forward (+)
20512573 .. 20513205
633 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g19890.1

Sequence Viewer

Length: 633 bp
ATGTATACCATGTCTATGAAGTTGATTGGGAGCATCTGCAGCTGCATATGGCTTGTCATGGCCGTATCAACCCTAGCCCAAACTTCCGATGACTCGAATGCTGTGCTTGACACTGCCGGAAAGGCTCTTCAATCCGGTGTGGACTACTACATCAATCCCGCTATTACCGACAGTGGCGGGCGTTTCACTTTGATCAACAGAACTGGATGTCCACTAAATGTTGGTCAAGAAAATGTCTCAGGTCCTGAGGGCTTGCCTGTGACGTTTTCTCCTTTTGTTGAGGGGGAAACTGTGGTGAGGGAAGGAAGGGACCAAAAGATCACATTCTCAGCATCCACAATCTGTGTGCAATCAACTGCATGGAAGGTGGGTGAGACCGATCAAGACACCCAAAGGAGATTAATTATCACCGGAGAAGACGAAAACCAAGGCCTTCCTGGCCCTGCCGGAAACTACTTCCGGATTACAAAGCAGGCAACACCAGATGGTGTGTACAATCTGGAATGGTGCCCTACTGAAGTGTGTCCAACTTGTAGGTTCATTTGTGGATCTGTTGGTGCTATTGTTGAGAATGACAAGAGGCTGCTGGCCTTGGATGGTTCAGCGCTTCCTGTTGTGTTTGAGAGGGCTTAA

Protein Analysis

211

Amino Acids

22.6

Weight (kDa)

4.57

Isoelectric Point (pI)

35.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kunitz_legume PF00197 35 - 208 8.6e-47 Trypsin and protease inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 507
AccI GTMKAC 1 cut(s) 5
AccIII TCCGGA 1 cut(s) 459
AciI CCGC 2 cut(s) 159, 177
AclWI GGATC 1 cut(s) 556
AcoI YGGCCR 1 cut(s) 60
AcuI CTGAAG 1 cut(s) 537
AfaI GTAC 1 cut(s) 494
AfeI AGCGCT 1 cut(s) 606
AgsI TTSAA 1 cut(s) 131
AjnI CCWGG 1 cut(s) 436
AloI GAACNNNNNNTCC 2 cut(s) 193, 225
AluBI AGCT 1 cut(s) 42
AluI AGCT 1 cut(s) 42
Alw26I GTCTC 2 cut(s) 241, 368
AlwI GGATC 1 cut(s) 556
AlwNI CAGNNNCTG 1 cut(s) 245
Aor13HI TCCGGA 1 cut(s) 459
Aor51HI AGCGCT 1 cut(s) 606
AoxI GGCC 4 cut(s) 60, 430, 439, 588
ApeKI GCWGC 3 cut(s) 39, 42, 583
AseI ATTAAT 1 cut(s) 401
AspLEI GCGC 1 cut(s) 607
AspS9I GGNCC 3 cut(s) 242, 310, 440
AsuHPI GGTGA 3 cut(s) 307, 383, 400
AvaII GGWCC 2 cut(s) 242, 310
AxyI CCTNAGG 1 cut(s) 246
BaeGI GKGCMC 1 cut(s) 512
BanI GGYRCC 1 cut(s) 507
BbsI GAAGAC 1 cut(s) 423
BbvI GCAGC 3 cut(s) 29, 51, 570
BccI CCATC 2 cut(s) 479, 590
BceAI ACGGC 1 cut(s) 47
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BciT130I CCWGG 1 cut(s) 438
BclI TGATCA 1 cut(s) 192
BcoDI GTCTC 2 cut(s) 241, 368
BfaI CTAG 1 cut(s) 74
BfmI CTRYAG 1 cut(s) 37
BfoI RGCGCY 1 cut(s) 608
BglI GCCNNNNNGGC 2 cut(s) 122, 438
BisI GCNGC 3 cut(s) 40, 43, 584
BlsI GCNGC 3 cut(s) 41, 44, 585
Bme1390I CCNGG 1 cut(s) 438
Bme18I GGWCC 2 cut(s) 242, 310
BmgT120I GGNCC 3 cut(s) 242, 310, 440
BmiI GGNNCC 2 cut(s) 311, 509
BmrFI CCNGG 1 cut(s) 438
BmsI GCATC 2 cut(s) 42, 341
BpiI GAAGAC 1 cut(s) 423
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 2 cut(s) 427, 591
BsaWI WCCGGW 3 cut(s) 134, 410, 459
BsaXI ACNNNNNCTCC 2 cut(s) 253, 283
Bse1I ACTGG 1 cut(s) 208
Bse21I CCTNAGG 1 cut(s) 246
BseAI TCCGGA 1 cut(s) 459
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 2 cut(s) 427, 591
BseGI GGATG 3 cut(s) 212, 332, 601
BseMII CTCAG 3 cut(s) 237, 252, 342
BseNI ACTGG 1 cut(s) 208
BseSI GKGCMC 1 cut(s) 512
BseXI GCAGC 3 cut(s) 29, 51, 570
BshFI GGCC 4 cut(s) 62, 432, 441, 590
BshNI GGYRCC 1 cut(s) 507
BsiSI CCGG 5 cut(s) 117, 135, 411, 447, 460
BslFI GGGAC 1 cut(s) 323
BsmAI GTCTC 2 cut(s) 241, 368
BsmFI GGGAC 1 cut(s) 323
BsmI GAATGC 1 cut(s) 103
BsnI GGCC 4 cut(s) 62, 432, 441, 590
Bso31I GGTCTC 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 512
Bsp13I TCCGGA 1 cut(s) 459
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 4 cut(s) 192, 318, 379, 548
BspACI CCGC 2 cut(s) 159, 177
BspANI GGCC 4 cut(s) 62, 432, 441, 590
BspCNI CTCAG 3 cut(s) 238, 251, 341
BspEI TCCGGA 1 cut(s) 459
BspLI GGNNCC 2 cut(s) 311, 509
BspMAI CTGCAG 1 cut(s) 41
BspPI GGATC 1 cut(s) 556
BspQI GCTCTTC 1 cut(s) 132
BspT107I GGYRCC 1 cut(s) 507
BspTNI GGTCTC 1 cut(s) 368
BsrGI TGTACA 1 cut(s) 492
BsrI ACTGG 1 cut(s) 208
BssECI CCNNGG 2 cut(s) 427, 591
BssMI GATC 4 cut(s) 192, 318, 379, 548
BssNAI GTATAC 1 cut(s) 6
BssT1I CCWWGG 2 cut(s) 427, 591
Bst1107I GTATAC 1 cut(s) 6
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 2 cut(s) 173, 292
Bst6I CTCTTC 1 cut(s) 132
BstAUI TGTACA 1 cut(s) 492
BstC8I GCNNGC 4 cut(s) 179, 254, 474, 588
BstDEI CTNAG 3 cut(s) 238, 246, 328
BstF5I GGATG 3 cut(s) 212, 332, 601
BstH2I RGCGCY 1 cut(s) 608
BstHHI GCGC 1 cut(s) 607
BstKTI GATC 4 cut(s) 195, 321, 382, 551
BstMAI GTCTC 2 cut(s) 241, 368
BstMBI GATC 4 cut(s) 192, 318, 379, 548
BstMWI GCNNNNNNNGC 3 cut(s) 39, 122, 438
BstNI CCWGG 1 cut(s) 438
BstSCI CCNGG 1 cut(s) 436
BstSFI CTRYAG 1 cut(s) 37
BstSLI GKGCMC 1 cut(s) 512
BstV1I GCAGC 3 cut(s) 29, 51, 570
BstV2I GAAGAC 1 cut(s) 423
BstX2I RGATCY 1 cut(s) 548
BstYI RGATCY 1 cut(s) 548
BstZ17I GTATAC 1 cut(s) 6
Bsu36I CCTNAGG 1 cut(s) 246
BsuRI GGCC 4 cut(s) 62, 432, 441, 590
BtsCI GGATG 3 cut(s) 212, 332, 601
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 2 cut(s) 111, 178
Cac8I GCNNGC 4 cut(s) 179, 254, 474, 588
CaiI CAGNNNCTG 1 cut(s) 245
CfoI GCGC 1 cut(s) 607
Cfr13I GGNCC 3 cut(s) 242, 310, 440
Csp6I GTAC 1 cut(s) 493
CviAII CATG 3 cut(s) 10, 58, 360
CviQI GTAC 1 cut(s) 493
DdeI CTNAG 3 cut(s) 238, 246, 328
DpnI GATC 4 cut(s) 194, 320, 381, 550
DpnII GATC 4 cut(s) 192, 318, 379, 548
EaeI YGGCCR 1 cut(s) 60
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Eco130I CCWWGG 2 cut(s) 427, 591
Eco147I AGGCCT 1 cut(s) 432
Eco31I GGTCTC 1 cut(s) 368
Eco47I GGWCC 2 cut(s) 242, 310
Eco47III AGCGCT 1 cut(s) 606
Eco57I CTGAAG 1 cut(s) 537
Eco81I CCTNAGG 1 cut(s) 246
EcoO109I RGGNCCY 1 cut(s) 242
EcoRII CCWGG 1 cut(s) 436
EcoT14I CCWWGG 2 cut(s) 427, 591
ErhI CCWWGG 2 cut(s) 427, 591
FaeI CATG 3 cut(s) 13, 61, 363
FaiI YATR 7 cut(s) 6, 11, 17, 47, 49, 59, 361
FaqI GGGAC 1 cut(s) 323
FatI CATG 3 cut(s) 9, 57, 359
FauI CCCGC 2 cut(s) 166, 170
FauNDI CATATG 1 cut(s) 47
FbaI TGATCA 1 cut(s) 192
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 3 cut(s) 40, 43, 584
FokI GGATG 3 cut(s) 219, 319, 608
Fsp4HI GCNGC 3 cut(s) 40, 43, 584
FspBI CTAG 1 cut(s) 74
GlaI GCGC 1 cut(s) 606
GluI GCNGC 3 cut(s) 40, 43, 584
HaeII RGCGCY 1 cut(s) 608
HaeIII GGCC 4 cut(s) 62, 432, 441, 590
HapII CCGG 5 cut(s) 117, 135, 411, 447, 460
HhaI GCGC 1 cut(s) 607
Hin1II CATG 3 cut(s) 13, 61, 363
Hin6I GCGC 1 cut(s) 605
HinP1I GCGC 1 cut(s) 605
HinfI GANTC 1 cut(s) 92
HpaII CCGG 5 cut(s) 117, 135, 411, 447, 460
HphI GGTGA 3 cut(s) 307, 383, 400
Hpy166II GTNNAC 4 cut(s) 6, 142, 212, 493
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 5 cut(s) 227, 245, 383, 460, 500
Hpy8I GTNNAC 4 cut(s) 6, 142, 212, 493
HpyAV CCTTC 4 cut(s) 296, 300, 358, 443
HpyCH4III ACNGT 2 cut(s) 173, 292
HpyCH4IV ACGT 1 cut(s) 263
HpyCH4V TGCA 4 cut(s) 39, 45, 349, 359
HpyF10VI GCNNNNNNNGC 3 cut(s) 39, 122, 438
HpyF3I CTNAG 3 cut(s) 238, 246, 328
HpySE526I ACGT 1 cut(s) 263
Hsp92II CATG 3 cut(s) 13, 61, 363
HspAI GCGC 1 cut(s) 605
Kpn2I TCCGGA 1 cut(s) 459
Ksp22I TGATCA 1 cut(s) 192
Kzo9I GATC 4 cut(s) 192, 318, 379, 548
LguI GCTCTTC 1 cut(s) 132
LmnI GCTCC 1 cut(s) 30
Lsp1109I GCAGC 3 cut(s) 29, 51, 570
LweI GCATC 2 cut(s) 42, 341
MaeI CTAG 1 cut(s) 74
MaeII ACGT 1 cut(s) 263
MaeIII GTNAC 1 cut(s) 259
MalI GATC 4 cut(s) 194, 320, 381, 550
MboI GATC 4 cut(s) 192, 318, 379, 548
MboII GAAGA 2 cut(s) 119, 428
MflI RGATCY 1 cut(s) 548
MhlI GDGCHC 1 cut(s) 512
MluCI AATT 1 cut(s) 402
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 1 cut(s) 551
MnlI CCTC 5 cut(s) 241, 274, 291, 573, 618
MroI TCCGGA 1 cut(s) 459
MseI TTAA 2 cut(s) 401, 631
MslI CAYNNNNRTG 1 cut(s) 14
MspA1I CMGCKG 1 cut(s) 42
MspI CCGG 5 cut(s) 117, 135, 411, 447, 460
MspR9I CCNGG 1 cut(s) 438
Mva1269I GAATGC 1 cut(s) 103
MvaI CCWGG 1 cut(s) 438
MwoI GCNNNNNNNGC 3 cut(s) 39, 122, 438
NdeI CATATG 1 cut(s) 47
NdeII GATC 4 cut(s) 192, 318, 379, 548
NlaIII CATG 3 cut(s) 13, 61, 363
NlaIV GGNNCC 2 cut(s) 311, 509
NmuCI GTSAC 1 cut(s) 259
PceI AGGCCT 1 cut(s) 432
PciSI GCTCTTC 1 cut(s) 132
PctI GAATGC 1 cut(s) 103
PkrI GCNGC 3 cut(s) 41, 44, 585
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
PpuMI RGGWCCY 1 cut(s) 242
PshBI ATTAAT 1 cut(s) 401
Psp5II RGGWCCY 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 2 cut(s) 311, 509
PspPI GGNCC 3 cut(s) 242, 310, 440
PspPPI RGGWCCY 1 cut(s) 242
PstI CTGCAG 1 cut(s) 41
PstNI CAGNNNCTG 1 cut(s) 245
PsuI RGATCY 1 cut(s) 548
PvuII CAGCTG 1 cut(s) 42
RsaI GTAC 1 cut(s) 494
RsaNI GTAC 1 cut(s) 493
RseI CAYNNNNRTG 1 cut(s) 14
SapI GCTCTTC 1 cut(s) 132
SaqAI TTAA 2 cut(s) 401, 631
SatI GCNGC 3 cut(s) 40, 43, 584
Sau3AI GATC 4 cut(s) 192, 318, 379, 548
Sau96I GGNCC 3 cut(s) 242, 310, 440
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 1 cut(s) 438
SduI GDGCHC 1 cut(s) 512
SetI ASST 5 cut(s) 44, 244, 266, 369, 539
SfaNI GCATC 2 cut(s) 42, 341
SfcI CTRYAG 1 cut(s) 37
SfiI GGCCNNNNNGGCC 1 cut(s) 438
SinI GGWCC 2 cut(s) 242, 310
SmiMI CAYNNNNRTG 1 cut(s) 14
Sse9I AATT 1 cut(s) 402
SseBI AGGCCT 1 cut(s) 432
SsiI CCGC 2 cut(s) 159, 177
SspMI CTAG 1 cut(s) 74
StuI AGGCCT 1 cut(s) 432
StyD4I CCNGG 1 cut(s) 436
StyI CCWWGG 2 cut(s) 427, 591
TaaI ACNGT 2 cut(s) 173, 292
TaiI ACGT 1 cut(s) 266
TaqI TCGA 1 cut(s) 95
TaqII GACCGA 1 cut(s) 392
TasI AATT 1 cut(s) 402
TatI WGTACW 1 cut(s) 492
Tru1I TTAA 2 cut(s) 401, 631
Tru9I TTAA 2 cut(s) 401, 631
TscAI CASTG 2 cut(s) 118, 178
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 3 cut(s) 39, 42, 583
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 2 cut(s) 32, 529
TspRI CASTG 2 cut(s) 118, 178
VpaK11BI GGWCC 2 cut(s) 242, 310
VspI ATTAAT 1 cut(s) 401
XcmI CCANNNNNNNNNTGG 1 cut(s) 434
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.