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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 200 |
| AciI | CCGC | 4 cut(s) | 11, 31, 236, 369 |
| AdeI | CACNNNGTG | 1 cut(s) | 284 |
| AfaI | GTAC | 2 cut(s) | 58, 64 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 101 |
| AgsI | TTSAA | 2 cut(s) | 28, 136 |
| AjiI | CACGTC | 1 cut(s) | 289 |
| AjnI | CCWGG | 3 cut(s) | 13, 94, 331 |
| AluBI | AGCT | 1 cut(s) | 311 |
| AluI | AGCT | 1 cut(s) | 311 |
| Alw26I | GTCTC | 1 cut(s) | 333 |
| AoxI | GGCC | 1 cut(s) | 109 |
| ApeKI | GCWGC | 2 cut(s) | 308, 353 |
| BbvI | GCAGC | 2 cut(s) | 320, 340 |
| BccI | CCATC | 2 cut(s) | 74, 120 |
| BciT130I | CCWGG | 3 cut(s) | 15, 96, 333 |
| BcoDI | GTCTC | 1 cut(s) | 333 |
| BfmI | CTRYAG | 1 cut(s) | 306 |
| BisI | GCNGC | 2 cut(s) | 309, 354 |
| BlsI | GCNGC | 2 cut(s) | 310, 355 |
| Bme1390I | CCNGG | 3 cut(s) | 15, 96, 333 |
| BmgBI | CACGTC | 1 cut(s) | 289 |
| BmrFI | CCNGG | 3 cut(s) | 15, 96, 333 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 101 |
| BseBI | CCWGG | 3 cut(s) | 15, 96, 333 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 101 |
| BseMII | CTCAG | 1 cut(s) | 315 |
| BseXI | GCAGC | 2 cut(s) | 320, 340 |
| BshFI | GGCC | 1 cut(s) | 111 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 101 |
| BsmAI | GTCTC | 1 cut(s) | 333 |
| BsnI | GGCC | 1 cut(s) | 111 |
| Bsp143I | GATC | 1 cut(s) | 138 |
| BspACI | CCGC | 4 cut(s) | 11, 31, 236, 369 |
| BspANI | GGCC | 1 cut(s) | 111 |
| BspCNI | CTCAG | 1 cut(s) | 316 |
| BspMAI | CTGCAG | 1 cut(s) | 310 |
| BssMI | GATC | 1 cut(s) | 138 |
| Bst2UI | CCWGG | 3 cut(s) | 15, 96, 333 |
| Bst4CI | ACNGT | 1 cut(s) | 53 |
| BstC8I | GCNNGC | 2 cut(s) | 11, 234 |
| BstDEI | CTNAG | 2 cut(s) | 324, 378 |
| BstKTI | GATC | 1 cut(s) | 141 |
| BstMAI | GTCTC | 1 cut(s) | 333 |
| BstMBI | GATC | 1 cut(s) | 138 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 233 |
| BstNI | CCWGG | 3 cut(s) | 15, 96, 333 |
| BstSCI | CCNGG | 3 cut(s) | 13, 94, 331 |
| BstSFI | CTRYAG | 1 cut(s) | 306 |
| BstV1I | GCAGC | 2 cut(s) | 320, 340 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 73 |
| BsuRI | GGCC | 1 cut(s) | 111 |
| BtrI | CACGTC | 1 cut(s) | 289 |
| BtsIMutI | CAGTG | 1 cut(s) | 58 |
| Cac8I | GCNNGC | 2 cut(s) | 11, 234 |
| CsiI | ACCWGGT | 1 cut(s) | 94 |
| Csp6I | GTAC | 2 cut(s) | 57, 63 |
| CviJI | RGCY | 4 cut(s) | 89, 111, 311, 353 |
| CviKI_1 | RGCY | 4 cut(s) | 89, 111, 311, 353 |
| CviQI | GTAC | 2 cut(s) | 57, 63 |
| DdeI | CTNAG | 2 cut(s) | 324, 378 |
| DpnI | GATC | 1 cut(s) | 140 |
| DpnII | GATC | 1 cut(s) | 138 |
| DraIII | CACNNNGTG | 1 cut(s) | 284 |
| EcoRII | CCWGG | 3 cut(s) | 13, 94, 331 |
| FaiI | YATR | 4 cut(s) | 74, 200, 248, 275 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 99, 131 |
| FauI | CCCGC | 2 cut(s) | 18, 376 |
| Fnu4HI | GCNGC | 2 cut(s) | 309, 354 |
| Fsp4HI | GCNGC | 2 cut(s) | 309, 354 |
| GluI | GCNGC | 2 cut(s) | 309, 354 |
| HaeIII | GGCC | 1 cut(s) | 111 |
| HinfI | GANTC | 1 cut(s) | 326 |
| Hpy188I | TCNGA | 1 cut(s) | 325 |
| Hpy188III | TCNNGA | 3 cut(s) | 116, 284, 314 |
| HpyAV | CCTTC | 4 cut(s) | 101, 148, 289, 357 |
| HpyCH4III | ACNGT | 1 cut(s) | 53 |
| HpyCH4IV | ACGT | 1 cut(s) | 288 |
| HpyCH4V | TGCA | 1 cut(s) | 308 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 233 |
| HpyF3I | CTNAG | 2 cut(s) | 324, 378 |
| HpySE526I | ACGT | 1 cut(s) | 288 |
| Kzo9I | GATC | 1 cut(s) | 138 |
| LmnI | GCTCC | 2 cut(s) | 178, 316 |
| Lsp1109I | GCAGC | 2 cut(s) | 320, 340 |
| MabI | ACCWGGT | 1 cut(s) | 94 |
| MaeII | ACGT | 1 cut(s) | 288 |
| MaeIII | GTNAC | 2 cut(s) | 208, 284 |
| MalI | GATC | 1 cut(s) | 140 |
| MboI | GATC | 1 cut(s) | 138 |
| MboII | GAAGA | 2 cut(s) | 148, 304 |
| MluCI | AATT | 1 cut(s) | 195 |
| MlyI | GAGTC | 1 cut(s) | 335 |
| MnlI | CCTC | 3 cut(s) | 143, 207, 372 |
| MspA1I | CMGCKG | 1 cut(s) | 236 |
| MspR9I | CCNGG | 3 cut(s) | 15, 96, 333 |
| MvaI | CCWGG | 3 cut(s) | 15, 96, 333 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 233 |
| NdeII | GATC | 1 cut(s) | 138 |
| NmuCI | GTSAC | 2 cut(s) | 208, 284 |
| PfoI | TCCNGGA | 1 cut(s) | 331 |
| PkrI | GCNGC | 2 cut(s) | 310, 355 |
| PleI | GAGTC | 1 cut(s) | 334 |
| PpsI | GAGTC | 1 cut(s) | 334 |
| PsiI | TTATAA | 1 cut(s) | 200 |
| Psp6I | CCWGG | 3 cut(s) | 13, 94, 331 |
| PspGI | CCWGG | 3 cut(s) | 13, 94, 331 |
| PstI | CTGCAG | 1 cut(s) | 310 |
| RsaI | GTAC | 2 cut(s) | 58, 64 |
| RsaNI | GTAC | 2 cut(s) | 57, 63 |
| SatI | GCNGC | 2 cut(s) | 309, 354 |
| Sau3AI | GATC | 1 cut(s) | 138 |
| SchI | GAGTC | 1 cut(s) | 335 |
| ScrFI | CCNGG | 3 cut(s) | 15, 96, 333 |
| SetI | ASST | 5 cut(s) | 19, 97, 225, 291, 313 |
| SexAI | ACCWGGT | 1 cut(s) | 94 |
| SfcI | CTRYAG | 1 cut(s) | 306 |
| Sse9I | AATT | 1 cut(s) | 195 |
| SsiI | CCGC | 4 cut(s) | 11, 31, 236, 369 |
| StyD4I | CCNGG | 3 cut(s) | 13, 94, 331 |
| TaaI | ACNGT | 1 cut(s) | 53 |
| TaiI | ACGT | 1 cut(s) | 291 |
| TasI | AATT | 1 cut(s) | 195 |
| TscAI | CASTG | 1 cut(s) | 58 |
| TseFI | GTSAC | 2 cut(s) | 208, 284 |
| TseI | GCWGC | 2 cut(s) | 308, 353 |
| Tsp45I | GTSAC | 2 cut(s) | 208, 284 |
| TspDTI | ATGAA | 1 cut(s) | 230 |
| TspRI | CASTG | 1 cut(s) | 58 |