DNA binding domain with preference for A/T rich regions
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 403 |
| AciI | CCGC | 3 cut(s) | 87, 195, 263 |
| AclWI | GGATC | 1 cut(s) | 395 |
| AfaI | GTAC | 2 cut(s) | 130, 136 |
| AgsI | TTSAA | 2 cut(s) | 28, 112 |
| AjnI | CCWGG | 2 cut(s) | 13, 94 |
| AlwI | GGATC | 1 cut(s) | 395 |
| AspS9I | GGNCC | 3 cut(s) | 98, 155, 307 |
| AsuHPI | GGTGA | 3 cut(s) | 223, 307, 391 |
| AsuII | TTCGAA | 1 cut(s) | 202 |
| AvaII | GGWCC | 3 cut(s) | 98, 155, 307 |
| BbvCI | CCTCAGC | 1 cut(s) | 375 |
| BccI | CCATC | 2 cut(s) | 185, 286 |
| BceAI | ACGGC | 1 cut(s) | 308 |
| BciT130I | CCWGG | 2 cut(s) | 15, 96 |
| BfmI | CTRYAG | 1 cut(s) | 396 |
| BfuAI | ACCTGC | 1 cut(s) | 403 |
| Bme1390I | CCNGG | 2 cut(s) | 15, 96 |
| Bme18I | GGWCC | 3 cut(s) | 98, 155, 307 |
| BmgT120I | GGNCC | 3 cut(s) | 98, 155, 307 |
| BmiI | GGNNCC | 1 cut(s) | 100 |
| BmrFI | CCNGG | 2 cut(s) | 15, 96 |
| Bpu10I | CCTNAGC | 1 cut(s) | 375 |
| Bpu14I | TTCGAA | 1 cut(s) | 202 |
| BseBI | CCWGG | 2 cut(s) | 15, 96 |
| BseMII | CTCAG | 3 cut(s) | 219, 363, 366 |
| BslFI | GGGAC | 1 cut(s) | 242 |
| BsmFI | GGGAC | 1 cut(s) | 242 |
| BsmI | GAATGC | 2 cut(s) | 85, 328 |
| Bsp119I | TTCGAA | 1 cut(s) | 202 |
| Bsp143I | GATC | 1 cut(s) | 400 |
| BspACI | CCGC | 3 cut(s) | 87, 195, 263 |
| BspCNI | CTCAG | 3 cut(s) | 220, 362, 367 |
| BspLI | GGNNCC | 1 cut(s) | 100 |
| BspMAI | CTGCAG | 1 cut(s) | 400 |
| BspMI | ACCTGC | 1 cut(s) | 403 |
| BspPI | GGATC | 1 cut(s) | 395 |
| BspT104I | TTCGAA | 1 cut(s) | 202 |
| BssMI | GATC | 1 cut(s) | 400 |
| Bst2UI | CCWGG | 2 cut(s) | 15, 96 |
| Bst6I | CTCTTC | 2 cut(s) | 69, 230 |
| BstBI | TTCGAA | 1 cut(s) | 202 |
| BstDEI | CTNAG | 4 cut(s) | 228, 349, 375, 405 |
| BstKTI | GATC | 1 cut(s) | 403 |
| BstMBI | GATC | 1 cut(s) | 400 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 320 |
| BstNI | CCWGG | 2 cut(s) | 15, 96 |
| BstSCI | CCNGG | 2 cut(s) | 13, 94 |
| BstSFI | CTRYAG | 1 cut(s) | 396 |
| BstX2I | RGATCY | 1 cut(s) | 400 |
| BstYI | RGATCY | 1 cut(s) | 400 |
| BveI | ACCTGC | 1 cut(s) | 403 |
| Cfr13I | GGNCC | 3 cut(s) | 98, 155, 307 |
| CsiI | ACCWGGT | 1 cut(s) | 13 |
| Csp6I | GTAC | 2 cut(s) | 129, 135 |
| CviAII | CATG | 2 cut(s) | 56, 71 |
| CviJI | RGCY | 2 cut(s) | 94, 323 |
| CviKI_1 | RGCY | 2 cut(s) | 94, 323 |
| CviQI | GTAC | 2 cut(s) | 129, 135 |
| DdeI | CTNAG | 4 cut(s) | 228, 349, 375, 405 |
| DpnI | GATC | 1 cut(s) | 402 |
| DpnII | GATC | 1 cut(s) | 400 |
| Eam1104I | CTCTTC | 2 cut(s) | 69, 230 |
| EarI | CTCTTC | 2 cut(s) | 69, 230 |
| EciI | GGCGGA | 1 cut(s) | 252 |
| Eco47I | GGWCC | 3 cut(s) | 98, 155, 307 |
| EcoO109I | RGGNCCY | 1 cut(s) | 307 |
| EcoRII | CCWGG | 2 cut(s) | 13, 94 |
| FaeI | CATG | 2 cut(s) | 59, 74 |
| FaiI | YATR | 3 cut(s) | 57, 72, 317 |
| FaqI | GGGAC | 1 cut(s) | 242 |
| FatI | CATG | 2 cut(s) | 55, 70 |
| FauI | CCCGC | 1 cut(s) | 188 |
| Hin1II | CATG | 2 cut(s) | 59, 74 |
| HinfI | GANTC | 1 cut(s) | 5 |
| HphI | GGTGA | 3 cut(s) | 223, 307, 391 |
| Hpy166II | GTNNAC | 1 cut(s) | 41 |
| Hpy188I | TCNGA | 1 cut(s) | 67 |
| Hpy188III | TCNNGA | 1 cut(s) | 109 |
| Hpy8I | GTNNAC | 1 cut(s) | 41 |
| Hpy99I | CGWCG | 1 cut(s) | 51 |
| HpyAV | CCTTC | 2 cut(s) | 202, 208 |
| HpyCH4IV | ACGT | 3 cut(s) | 37, 49, 370 |
| HpyCH4V | TGCA | 4 cut(s) | 83, 245, 328, 398 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 320 |
| HpyF3I | CTNAG | 4 cut(s) | 228, 349, 375, 405 |
| HpySE526I | ACGT | 3 cut(s) | 37, 49, 370 |
| Hsp92II | CATG | 2 cut(s) | 59, 74 |
| Kzo9I | GATC | 1 cut(s) | 400 |
| LpnPI | CCDG | 6 cut(s) | 27, 81, 108, 171, 294, 333 |
| MabI | ACCWGGT | 1 cut(s) | 13 |
| MaeII | ACGT | 3 cut(s) | 37, 49, 370 |
| MaeIII | GTNAC | 1 cut(s) | 295 |
| MalI | GATC | 1 cut(s) | 402 |
| MboI | GATC | 1 cut(s) | 400 |
| MboII | GAAGA | 4 cut(s) | 86, 191, 247, 324 |
| MflI | RGATCY | 1 cut(s) | 400 |
| MnlI | CCTC | 4 cut(s) | 142, 270, 357, 370 |
| MseI | TTAA | 2 cut(s) | 165, 288 |
| MspR9I | CCNGG | 2 cut(s) | 15, 96 |
| Mva1269I | GAATGC | 2 cut(s) | 85, 328 |
| MvaI | CCWGG | 2 cut(s) | 15, 96 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 320 |
| NdeII | GATC | 1 cut(s) | 400 |
| NlaIII | CATG | 2 cut(s) | 59, 74 |
| NlaIV | GGNNCC | 1 cut(s) | 100 |
| NmuCI | GTSAC | 1 cut(s) | 295 |
| NspV | TTCGAA | 1 cut(s) | 202 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 43 |
| PctI | GAATGC | 2 cut(s) | 85, 328 |
| PfeI | GAWTC | 1 cut(s) | 5 |
| PpuMI | RGGWCCY | 1 cut(s) | 307 |
| Psp5II | RGGWCCY | 1 cut(s) | 307 |
| Psp6I | CCWGG | 2 cut(s) | 13, 94 |
| PspGI | CCWGG | 2 cut(s) | 13, 94 |
| PspN4I | GGNNCC | 1 cut(s) | 100 |
| PspPI | GGNCC | 3 cut(s) | 98, 155, 307 |
| PspPPI | RGGWCCY | 1 cut(s) | 307 |
| PstI | CTGCAG | 1 cut(s) | 400 |
| PsuI | RGATCY | 1 cut(s) | 400 |
| RsaI | GTAC | 2 cut(s) | 130, 136 |
| RsaNI | GTAC | 2 cut(s) | 129, 135 |
| SaqAI | TTAA | 2 cut(s) | 165, 288 |
| Sau3AI | GATC | 1 cut(s) | 400 |
| Sau96I | GGNCC | 3 cut(s) | 98, 155, 307 |
| ScrFI | CCNGG | 2 cut(s) | 15, 96 |
| SexAI | ACCWGGT | 1 cut(s) | 13 |
| SfcI | CTRYAG | 1 cut(s) | 396 |
| SfuI | TTCGAA | 1 cut(s) | 202 |
| SinI | GGWCC | 3 cut(s) | 98, 155, 307 |
| SsiI | CCGC | 3 cut(s) | 87, 195, 263 |
| SspI | AATATT | 1 cut(s) | 185 |
| StyD4I | CCNGG | 2 cut(s) | 13, 94 |
| TaiI | ACGT | 3 cut(s) | 40, 52, 373 |
| TaqI | TCGA | 3 cut(s) | 46, 189, 202 |
| TatI | WGTACW | 2 cut(s) | 128, 134 |
| TfiI | GAWTC | 1 cut(s) | 5 |
| Tru1I | TTAA | 2 cut(s) | 165, 288 |
| Tru9I | TTAA | 2 cut(s) | 165, 288 |
| TseFI | GTSAC | 1 cut(s) | 295 |
| Tsp45I | GTSAC | 1 cut(s) | 295 |
| TspDTI | ATGAA | 4 cut(s) | 87, 221, 248, 332 |
| VpaK11BI | GGWCC | 3 cut(s) | 98, 155, 307 |