Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001458

Physical Location & Seq

Genomic Coordinates Forward (+)

40179494 .. 40181519

Gene Structure 2026 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1319700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 603 bp

ATGTTGATCGCTAATTTTTGTGGGCTGGCCCAAAGACTTGGAAGCTTCTACAATCTACTACTTACCGCCAGCTTCTCAATTAGTAGCCCCCCTCTCTCTCTGAGTTGCAGAGACCTCCGTCGCTACGACGCTCACCTCTTCCAACGACGACGTTTCAGTAGCCAGCCACATTACGGGCGAGCTTGCTCGATGGATATAATCTCCCAATTACAGGGACAAGTGAACGCAATTGCTTCTCTTACCTTCAATACCTTTGGGACACTACAAAGGGATTCTCCTCCAGTCCGACTTTCACCAAATTACCCAGAACCACCAGCTAATCCTACCGAGGATGCTGCTGATTTTGCCCAACAACCCAAGCTCATGGGCGCAGCTCTTGTTAAGGCTGCTAAGCAGTTTGATGCATTAGTTGCAGCACTTCCATTAGCCGATGGAGGTGAAGAAGGGCAGCTGAAACGGATTGCAGAACTGCAGGCCGAAAATGATGCTGTCGGCCAAGAACTCCAGAGACAACTCGAAGCTGCAGGTAAAGAATTAAGGGAGGTCCGCGAATTGTTCGGCCAATCGGTAGAAAATTGCTTGAACCTGAAGAAACCTGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009814 GO:0009817 GO:0009889 GO:0010468 GO:0010556 GO:0016592 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032991 GO:0043076 GO:0043078 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045087 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0070013 GO:0080090 GO:0098542 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Med21

Protein Analysis

201

Amino Acids

21.98

Weight (kDa)

6.16

Isoelectric Point (pI)

63.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Med21	PF11221	64 - 188	4e-24	Subunit 21 of Mediator complex

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001458)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04780
fragaria_vesca	FvH4_3g18180 FvH4_3g18181 FvH4_4g21200
malus_domestica	MD10G1319700.v1.1
prunus_persica	Prupe.1G108500_v2.0.a1 Prupe.1G108500_v2.0.a1 Prupe.1G108500_v2.0.a1 Prupe.1G108500_v2.0.a1 Prupe.1G108500_v2.0.a1 Prupe.4G022700_v2.0.a1 Prupe.4G022700_v2.0.a1 Prupe.4G022700_v2.0.a1 Prupe.4G022700_v2.0.a1 Prupe.4G022700_v2.0.a1 Prupe.4G022700_v2.0.a1 Prupe.4G022700_v2.0.a1
pyrus_communis	pycom10g27060 pycom16g12330
rosa_chinensis	RchiOBHm_Chr4g0426811 RchiOBHm_Chr5g0030661 RchiOBHm_Chr5g0030681
rosa_laevigata	RLG00000007276 RLG00000033237 RLG00000033242 RLG00000033244
rosa_multiflora	Rmu_sc0000953.1_g000011 Rmu_sc0004341.1_g000003 Rmu_sc0004963.1_g000002
rosa_roxburghii	Rroxscaffold_1G00049620 Rroxscaffold_1G00049630 Rroxscaffold_1G00049640 Rroxscaffold_5G00368780
rosa_rugosa	Rorug04G0215000 Rorug05G0120100 Rorug05G0120100
rosa_samantha	Rh4AG270000 Rh4BG276300 Rh4CG291000 Rh4DG273500 Rh5AG211500 Rh5AG211700 Rh5BG210400 Rh5BG210600 Rh5CG234200 Rh5DG215500 Rh5DG215600 Rh5DG215800 Rh5DG215900
rosa_wichuraiana	Rw4G023430 Rw5G019370 Rw5G019410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	515
AccII	CGCG	1 cut(s)	549
AciI	CCGC	2 cut(s)	66, 547
AcoI	YGGCCR	2 cut(s)	493, 559
AfiI	CCNNNNNNNGG	2 cut(s)	173, 211
AgsI	TTSAA	2 cut(s)	247, 583
AluBI	AGCT	8 cut(s)	45, 72, 182, 317, 361, 374, 451, 521
AluI	AGCT	8 cut(s)	45, 72, 182, 317, 361, 374, 451, 521
Alw26I	GTCTC	2 cut(s)	105, 502
AoxI	GGCC	4 cut(s)	27, 474, 493, 559
ApeKI	GCWGC	6 cut(s)	335, 371, 386, 413, 448, 521
AspLEI	GCGC	1 cut(s)	371
AspS9I	GGNCC	2 cut(s)	28, 544
AsuHPI	GGTGA	3 cut(s)	125, 285, 449
AvaII	GGWCC	1 cut(s)	544
BbvI	GCAGC	6 cut(s)	322, 373, 383, 425, 460, 508
BccI	CCATC	2 cut(s)	184, 425
BcgI	CGANNNNNNTGC	4 cut(s)	317, 351, 467, 501
BcoDI	GTCTC	2 cut(s)	105, 502
BfmI	CTRYAG	2 cut(s)	470, 522
BfuAI	ACCTGC	1 cut(s)	515
BisI	GCNGC	6 cut(s)	336, 372, 387, 414, 449, 522
BlpI	GCTNAGC	1 cut(s)	390
BlsI	GCNGC	6 cut(s)	337, 373, 388, 415, 450, 523
Bme18I	GGWCC	1 cut(s)	544
BmgT120I	GGNCC	2 cut(s)	28, 544
BmsI	GCATC	3 cut(s)	322, 391, 475
BoxI	GACNNNNGTC	1 cut(s)	117
BpmI	CTGGAG	2 cut(s)	264, 488
Bpu1102I	GCTNAGC	1 cut(s)	390
BsaI	GGTCTC	1 cut(s)	105
BsaJI	CCNNGG	1 cut(s)	327
Bsc4I	CCNNNNNNNGG	2 cut(s)	173, 211
Bse1I	ACTGG	1 cut(s)	281
BseDI	CCNNGG	1 cut(s)	327
BseGI	GGATG	1 cut(s)	337
BseLI	CCNNNNNNNGG	2 cut(s)	173, 211
BseMII	CTCAG	1 cut(s)	92
BseNI	ACTGG	1 cut(s)	281
BseRI	GAGGAG	1 cut(s)	267
BseXI	GCAGC	6 cut(s)	322, 373, 383, 425, 460, 508
Bsh1236I	CGCG	1 cut(s)	549
BshFI	GGCC	4 cut(s)	29, 476, 495, 561
BslFI	GGGAC	2 cut(s)	228, 271
BslI	CCNNNNNNNGG	2 cut(s)	173, 211
BsmAI	GTCTC	2 cut(s)	105, 502
BsmFI	GGGAC	2 cut(s)	228, 271
BsnI	GGCC	4 cut(s)	29, 476, 495, 561
Bso31I	GGTCTC	1 cut(s)	105
Bsp143I	GATC	1 cut(s)	6
Bsp1720I	GCTNAGC	1 cut(s)	390
BspACI	CCGC	2 cut(s)	66, 547
BspANI	GGCC	4 cut(s)	29, 476, 495, 561
BspCNI	CTCAG	1 cut(s)	93
BspFNI	CGCG	1 cut(s)	549
BspMAI	CTGCAG	2 cut(s)	474, 526
BspMI	ACCTGC	1 cut(s)	515
BspTNI	GGTCTC	1 cut(s)	105
BsrI	ACTGG	1 cut(s)	281
BssECI	CCNNGG	1 cut(s)	327
BssMI	GATC	1 cut(s)	6
Bst6I	CTCTTC	1 cut(s)	143
BstAPI	GCANNNNNTGC	1 cut(s)	410
BstC8I	GCNNGC	6 cut(s)	27, 70, 164, 180, 184, 474
BstDEI	CTNAG	2 cut(s)	101, 390
BstF5I	GGATG	1 cut(s)	337
BstFNI	CGCG	1 cut(s)	549
BstHHI	GCGC	1 cut(s)	371
BstKTI	GATC	1 cut(s)	9
BstMAI	GTCTC	2 cut(s)	105, 502
BstMBI	GATC	1 cut(s)	6
BstMWI	GCNNNNNNNGC	2 cut(s)	344, 410
BstPAI	GACNNNNGTC	1 cut(s)	117
BstSFI	CTRYAG	2 cut(s)	470, 522
BstUI	CGCG	1 cut(s)	549
BstV1I	GCAGC	6 cut(s)	322, 373, 383, 425, 460, 508
BstXI	CCANNNNNNTGG	2 cut(s)	38, 364
BsuRI	GGCC	4 cut(s)	29, 476, 495, 561
BtsCI	GGATG	1 cut(s)	337
BveI	ACCTGC	1 cut(s)	515
Cac8I	GCNNGC	6 cut(s)	27, 70, 164, 180, 184, 474
CfoI	GCGC	1 cut(s)	371
Cfr13I	GGNCC	2 cut(s)	28, 544
CseI	GACGC	1 cut(s)	137
CviAII	CATG	1 cut(s)	364
DdeI	CTNAG	2 cut(s)	101, 390
DpnI	GATC	1 cut(s)	8
DpnII	GATC	1 cut(s)	6
EaeI	YGGCCR	2 cut(s)	493, 559
Eam1104I	CTCTTC	1 cut(s)	143
EarI	CTCTTC	1 cut(s)	143
Eco31I	GGTCTC	1 cut(s)	105
Eco47I	GGWCC	1 cut(s)	544
EcoT22I	ATGCAT	1 cut(s)	406
FaeI	CATG	1 cut(s)	367
FaiI	YATR	2 cut(s)	197, 365
FaqI	GGGAC	2 cut(s)	228, 271
FatI	CATG	1 cut(s)	363
Fnu4HI	GCNGC	6 cut(s)	336, 372, 387, 414, 449, 522
FokI	GGATG	1 cut(s)	344
Fsp4HI	GCNGC	6 cut(s)	336, 372, 387, 414, 449, 522
GlaI	GCGC	1 cut(s)	370
GluI	GCNGC	6 cut(s)	336, 372, 387, 414, 449, 522
GsuI	CTGGAG	2 cut(s)	264, 488
HaeIII	GGCC	4 cut(s)	29, 476, 495, 561
HgaI	GACGC	1 cut(s)	137
HhaI	GCGC	1 cut(s)	371
Hin1II	CATG	1 cut(s)	367
Hin6I	GCGC	1 cut(s)	369
HinP1I	GCGC	1 cut(s)	369
HindIII	AAGCTT	1 cut(s)	43
HinfI	GANTC	1 cut(s)	272
HphI	GGTGA	3 cut(s)	125, 285, 449
Hpy166II	GTNNAC	1 cut(s)	223
Hpy188I	TCNGA	2 cut(s)	102, 287
Hpy188III	TCNNGA	1 cut(s)	505
Hpy8I	GTNNAC	1 cut(s)	223
Hpy99I	CGWCG	4 cut(s)	123, 131, 150, 153
HpyAV	CCTTC	2 cut(s)	253, 437
HpyCH4IV	ACGT	1 cut(s)	151
HpyCH4V	TGCA	6 cut(s)	108, 404, 413, 464, 472, 524
HpyF10VI	GCNNNNNNNGC	2 cut(s)	344, 410
HpyF3I	CTNAG	2 cut(s)	101, 390
HpySE526I	ACGT	1 cut(s)	151
Hsp92II	CATG	1 cut(s)	367
HspAI	GCGC	1 cut(s)	369
Kzo9I	GATC	1 cut(s)	6
Lsp1109I	GCAGC	6 cut(s)	322, 373, 383, 425, 460, 508
LweI	GCATC	3 cut(s)	322, 391, 475
MaeII	ACGT	1 cut(s)	151
MalI	GATC	1 cut(s)	8
MboI	GATC	1 cut(s)	6
MboII	GAAGA	3 cut(s)	130, 452, 601
MfeI	CAATTG	1 cut(s)	228
MluCI	AATT	8 cut(s)	13, 78, 206, 228, 298, 533, 551, 574
MmeI	TCCRAC	2 cut(s)	166, 310
MnlI	CCTC	7 cut(s)	102, 125, 146, 288, 322, 428, 535
Mph1103I	ATGCAT	1 cut(s)	406
MseI	TTAA	2 cut(s)	381, 536
MspA1I	CMGCKG	1 cut(s)	451
MunI	CAATTG	1 cut(s)	228
MvnI	CGCG	1 cut(s)	549
MwoI	GCNNNNNNNGC	2 cut(s)	344, 410
NdeII	GATC	1 cut(s)	6
NlaIII	CATG	1 cut(s)	367
NsiI	ATGCAT	1 cut(s)	406
PfeI	GAWTC	1 cut(s)	272
PkrI	GCNGC	6 cut(s)	337, 373, 388, 415, 450, 523
PshAI	GACNNNNGTC	1 cut(s)	117
PspPI	GGNCC	2 cut(s)	28, 544
PstI	CTGCAG	2 cut(s)	474, 526
PvuII	CAGCTG	1 cut(s)	451
SaqAI	TTAA	2 cut(s)	381, 536
SatI	GCNGC	6 cut(s)	336, 372, 387, 414, 449, 522
Sau3AI	GATC	1 cut(s)	6
Sau96I	GGNCC	2 cut(s)	28, 544
SfaNI	GCATC	3 cut(s)	322, 391, 475
SfcI	CTRYAG	2 cut(s)	470, 522
SinI	GGWCC	1 cut(s)	544
Sse9I	AATT	8 cut(s)	13, 78, 206, 228, 298, 533, 551, 574
SsiI	CCGC	2 cut(s)	66, 547
TaiI	ACGT	1 cut(s)	154
TaqI	TCGA	2 cut(s)	188, 516
TasI	AATT	8 cut(s)	13, 78, 206, 228, 298, 533, 551, 574
TfiI	GAWTC	1 cut(s)	272
Tru1I	TTAA	2 cut(s)	381, 536
Tru9I	TTAA	2 cut(s)	381, 536
TseI	GCWGC	6 cut(s)	335, 371, 386, 413, 448, 521
TspGWI	ACGGA	2 cut(s)	107, 472
VpaK11BI	GGWCC	1 cut(s)	544
Zsp2I	ATGCAT	1 cut(s)	406

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.