Prupe.1G108500_v2.0.a1

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001458
Physical Location & Seq
Reverse (-)
8680115 .. 8682670
2556 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G108500.3

Sequence Viewer

Length: 495 bp
ATGGATGCAATCAGCCAATTACAAGAGAAAGTGAATACAATCGCGACAATCGCGTTCACTACCATCGGAACGCTGCAGAGGGATGCGCCGCCGGTCCGAATCTCTCCGAATTACCCAGAATCCGGATCTGGGCCGGCTCCGGCTGCGGCTCCAAATCCGAACCCGACCCCGACCCCGACCCCGACCCCGATAGCGGACAGTGACGCGGATTTCGCGAAGCAACCCAAGCTGATGAGTGCTGAGCTAGTGAAGGCAGCTAAGCAGTTTGATGCATTGGTGGCGGCACTACCGTTGTCTGAGGGGGGAGAGGAAGCTCAGCTGAAGAGGATTGCACAACTTGAGGCTGAAAATGATGCTGTGGGCCAACAACTTGAGAAGCAACTGGAAGCTGCAGTCAACGGCAGAATCTTTTTCCATCTTCCTTCTCTTTTGCCTTTTAAATCATCACATCAGATCAGAGAAGGGGCCTACGCAAATGACCACTATGGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Med21

Protein Analysis

165

Amino Acids

17.5

Weight (kDa)

5.16

Isoelectric Point (pI)

49.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 44, 53, 206, 215
AccIII TCCGGA 1 cut(s) 122
AciI CCGC 5 cut(s) 89, 146, 194, 206, 281
AclWI GGATC 1 cut(s) 133
AcuI CTGAAG 1 cut(s) 341
AfiI CCNNNNNNNGG 3 cut(s) 122, 129, 193
AluBI AGCT 6 cut(s) 229, 244, 257, 314, 319, 389
AluI AGCT 6 cut(s) 229, 244, 257, 314, 319, 389
AlwI GGATC 1 cut(s) 133
Aor13HI TCCGGA 1 cut(s) 122
AoxI GGCC 3 cut(s) 131, 361, 465
ApeKI GCWGC 4 cut(s) 73, 143, 254, 389
ArsI GACNNNNNNTTYG 2 cut(s) 194, 226
AspLEI GCGC 1 cut(s) 88
AspS9I GGNCC 4 cut(s) 94, 131, 361, 465
AvaII GGWCC 1 cut(s) 94
BbvI GCAGC 4 cut(s) 60, 130, 266, 376
BccI CCATC 2 cut(s) 71, 423
BceAI ACGGC 1 cut(s) 415
BfaI CTAG 1 cut(s) 245
BfmI CTRYAG 2 cut(s) 74, 390
BisI GCNGC 7 cut(s) 74, 89, 144, 147, 255, 282, 390
BlpI GCTNAGC 3 cut(s) 240, 258, 315
BlsI GCNGC 7 cut(s) 75, 90, 145, 148, 256, 283, 391
Bme18I GGWCC 1 cut(s) 94
BmgT120I GGNCC 4 cut(s) 94, 131, 361, 465
BmiI GGNNCC 3 cut(s) 138, 150, 466
BmsI GCATC 3 cut(s) 73, 259, 343
Bpu1102I GCTNAGC 3 cut(s) 240, 258, 315
BpuEI CTTGAG 2 cut(s) 359, 392
BsaWI WCCGGW 1 cut(s) 122
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 129, 193
Bse118I RCCGGY 2 cut(s) 91, 133
Bse1I ACTGG 1 cut(s) 387
BseAI TCCGGA 1 cut(s) 122
BseGI GGATG 2 cut(s) 10, 88
BseLI CCNNNNNNNGG 3 cut(s) 122, 129, 193
BseMII CTCAG 3 cut(s) 231, 288, 329
BseNI ACTGG 1 cut(s) 387
BseXI GCAGC 4 cut(s) 60, 130, 266, 376
Bsh1236I CGCG 4 cut(s) 44, 53, 206, 215
BshFI GGCC 3 cut(s) 133, 363, 467
BsiSI CCGG 4 cut(s) 92, 123, 134, 140
BslI CCNNNNNNNGG 3 cut(s) 122, 129, 193
BsnI GGCC 3 cut(s) 133, 363, 467
Bsp13I TCCGGA 1 cut(s) 122
Bsp143I GATC 2 cut(s) 125, 453
Bsp1720I GCTNAGC 3 cut(s) 240, 258, 315
Bsp68I TCGCGA 2 cut(s) 44, 215
BspACI CCGC 5 cut(s) 89, 146, 194, 206, 281
BspANI GGCC 3 cut(s) 133, 363, 467
BspCNI CTCAG 3 cut(s) 232, 289, 328
BspEI TCCGGA 1 cut(s) 122
BspFNI CGCG 4 cut(s) 44, 53, 206, 215
BspLI GGNNCC 3 cut(s) 138, 150, 466
BspMAI CTGCAG 2 cut(s) 78, 394
BspPI GGATC 1 cut(s) 133
BsrFI RCCGGY 2 cut(s) 91, 133
BsrI ACTGG 1 cut(s) 387
BssAI RCCGGY 2 cut(s) 91, 133
BssMI GATC 2 cut(s) 125, 453
Bst4CI ACNGT 2 cut(s) 200, 291
Bst6I CTCTTC 1 cut(s) 317
BstC8I GCNNGC 1 cut(s) 135
BstDEI CTNAG 4 cut(s) 240, 258, 297, 315
BstF5I GGATG 2 cut(s) 10, 88
BstFNI CGCG 4 cut(s) 44, 53, 206, 215
BstHHI GCGC 1 cut(s) 88
BstKTI GATC 2 cut(s) 128, 456
BstMBI GATC 2 cut(s) 125, 453
BstMWI GCNNNNNNNGC 5 cut(s) 50, 143, 212, 226, 278
BstSFI CTRYAG 2 cut(s) 74, 390
BstUI CGCG 4 cut(s) 44, 53, 206, 215
BstV1I GCAGC 4 cut(s) 60, 130, 266, 376
BstX2I RGATCY 1 cut(s) 125
BstYI RGATCY 1 cut(s) 125
BsuRI GGCC 3 cut(s) 133, 363, 467
BtsCI GGATG 2 cut(s) 10, 88
BtsIMutI CAGTG 1 cut(s) 205
BtuMI TCGCGA 2 cut(s) 44, 215
Cac8I GCNNGC 1 cut(s) 135
CfoI GCGC 1 cut(s) 88
Cfr10I RCCGGY 2 cut(s) 91, 133
Cfr13I GGNCC 4 cut(s) 94, 131, 361, 465
CpoI CGGWCCG 1 cut(s) 94
CseI GACGC 1 cut(s) 212
CspI CGGWCCG 1 cut(s) 94
DdeI CTNAG 4 cut(s) 240, 258, 297, 315
DpnI GATC 2 cut(s) 127, 455
DpnII GATC 2 cut(s) 125, 453
DraI TTTAAA 1 cut(s) 439
Eam1104I CTCTTC 1 cut(s) 317
EarI CTCTTC 1 cut(s) 317
Eco47I GGWCC 1 cut(s) 94
Eco57I CTGAAG 1 cut(s) 341
EcoO109I RGGNCCY 1 cut(s) 465
EcoT22I ATGCAT 1 cut(s) 274
FaiI YATR 1 cut(s) 486
Fnu4HI GCNGC 7 cut(s) 74, 89, 144, 147, 255, 282, 390
FokI GGATG 2 cut(s) 17, 95
Fsp4HI GCNGC 7 cut(s) 74, 89, 144, 147, 255, 282, 390
FspBI CTAG 1 cut(s) 245
GlaI GCGC 1 cut(s) 87
GluI GCNGC 7 cut(s) 74, 89, 144, 147, 255, 282, 390
HaeIII GGCC 3 cut(s) 133, 363, 467
HapII CCGG 4 cut(s) 92, 123, 134, 140
HgaI GACGC 1 cut(s) 212
HhaI GCGC 1 cut(s) 88
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HinfI GANTC 3 cut(s) 99, 119, 405
HpaII CCGG 4 cut(s) 92, 123, 134, 140
Hpy166II GTNNAC 2 cut(s) 57, 397
Hpy188I TCNGA 7 cut(s) 68, 98, 108, 159, 298, 453, 458
Hpy188III TCNNGA 3 cut(s) 43, 123, 214
Hpy8I GTNNAC 2 cut(s) 57, 397
HpyAV CCTTC 3 cut(s) 244, 432, 455
HpyCH4III ACNGT 2 cut(s) 200, 291
HpyCH4V TGCA 5 cut(s) 8, 76, 272, 332, 392
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 143, 212, 226, 278
HpyF3I CTNAG 4 cut(s) 240, 258, 297, 315
HspAI GCGC 1 cut(s) 86
Kpn2I TCCGGA 1 cut(s) 122
KroI GCCGGC 1 cut(s) 133
KroNI GCCGGC 1 cut(s) 135
Kzo9I GATC 2 cut(s) 125, 453
LmnI GCTCC 2 cut(s) 142, 154
LpnPI CCDG 7 cut(s) 105, 114, 129, 136, 147, 153, 368
Lsp1109I GCAGC 4 cut(s) 60, 130, 266, 376
LweI GCATC 3 cut(s) 73, 259, 343
MaeI CTAG 1 cut(s) 245
MaeIII GTNAC 1 cut(s) 200
MalI GATC 2 cut(s) 127, 455
MboI GATC 2 cut(s) 125, 453
MboII GAAGA 2 cut(s) 334, 410
MflI RGATCY 1 cut(s) 125
MluCI AATT 2 cut(s) 17, 109
MnlI CCTC 5 cut(s) 72, 292, 301, 318, 334
Mph1103I ATGCAT 1 cut(s) 274
MroI TCCGGA 1 cut(s) 122
MroNI GCCGGC 1 cut(s) 133
MseI TTAA 1 cut(s) 438
MspA1I CMGCKG 1 cut(s) 319
MspI CCGG 4 cut(s) 92, 123, 134, 140
MvnI CGCG 4 cut(s) 44, 53, 206, 215
MwoI GCNNNNNNNGC 5 cut(s) 50, 143, 212, 226, 278
NaeI GCCGGC 1 cut(s) 135
NdeII GATC 2 cut(s) 125, 453
NgoMIV GCCGGC 1 cut(s) 133
NlaIV GGNNCC 3 cut(s) 138, 150, 466
NmuCI GTSAC 1 cut(s) 200
NruI TCGCGA 2 cut(s) 44, 215
NsiI ATGCAT 1 cut(s) 274
PdiI GCCGGC 1 cut(s) 135
PfeI GAWTC 3 cut(s) 99, 119, 405
PkrI GCNGC 7 cut(s) 75, 90, 145, 148, 256, 283, 391
PspN4I GGNNCC 3 cut(s) 138, 150, 466
PspPI GGNCC 4 cut(s) 94, 131, 361, 465
PstI CTGCAG 2 cut(s) 78, 394
PsuI RGATCY 1 cut(s) 125
PvuII CAGCTG 1 cut(s) 319
RruI TCGCGA 2 cut(s) 44, 215
Rsr2I CGGWCCG 1 cut(s) 94
RsrII CGGWCCG 1 cut(s) 94
SaqAI TTAA 1 cut(s) 438
SatI GCNGC 7 cut(s) 74, 89, 144, 147, 255, 282, 390
Sau3AI GATC 2 cut(s) 125, 453
Sau96I GGNCC 4 cut(s) 94, 131, 361, 465
SetI ASST 6 cut(s) 231, 246, 259, 316, 321, 391
SfaNI GCATC 3 cut(s) 73, 259, 343
SfcI CTRYAG 2 cut(s) 74, 390
SinI GGWCC 1 cut(s) 94
SmlI CTYRAG 2 cut(s) 338, 371
SmoI CTYRAG 2 cut(s) 338, 371
Sse9I AATT 2 cut(s) 17, 109
SsiI CCGC 5 cut(s) 89, 146, 194, 206, 281
SspMI CTAG 1 cut(s) 245
TaaI ACNGT 2 cut(s) 200, 291
TasI AATT 2 cut(s) 17, 109
TauI GCSGC 3 cut(s) 91, 149, 284
TfiI GAWTC 3 cut(s) 99, 119, 405
Tru1I TTAA 1 cut(s) 438
Tru9I TTAA 1 cut(s) 438
TscAI CASTG 1 cut(s) 205
TseFI GTSAC 1 cut(s) 200
TseI GCWGC 4 cut(s) 73, 143, 254, 389
Tsp45I GTSAC 1 cut(s) 200
TspRI CASTG 1 cut(s) 205
VpaK11BI GGWCC 1 cut(s) 94
XspI CTAG 1 cut(s) 245
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.