FvH4_3g26900

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001599
Physical Location & Seq
Reverse (-)
20009550 .. 20011521
1972 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g26900.t1

Sequence Viewer

Length: 987 bp
ATGTCAAGCACTACTACTGAAACCACCAGTAGTACTAGCAGTTTGCCCTACGAAGAATGCCGGGGCGTTCTTCGTGTGTACAGCGACGGCTCCATCGTCCGTTCCTCCAAGCCAAGCTTCGACGTCCCTGTCCACGACGACGGCTCCGTCGCCTGGAAGGACGTAGTGTTCGACCACACTAACCAGCTTCAGCTCCGCCTCTACAAGCCGGCGGCTTCCAAATCCAAGCTACCAATATTCTACTACATCCACGGCGGTGGCTTCTGCATAGGTTCACGCGCCTGGCCCAACTGCCAGAACTACTGTTTCCAGCTCGCTCTCAGCCTCCAAGCAGTTATTGTCTCGCCGGACTACCGCCTGGCTCCCGAACACCGCCTCCCGGCTGCTATTGACGACGGTTTCGCCGCCGTTAAGTGGCTTCAAGCCCAAGCCTTGTCGTCGGAGGACACTTGGTTGACTGACGTGGCTGACTTTGGTAACGTGTTCATTTCTGGTGACTCGGCCGGCGGCAACATTGCTCATAATTTGGCGGTTCAACTCGGGGCCGGTTCGCCTGAGCTGGCTCCGGTTCGGGTCAAGGGTTATGTGCTCTTGGCTCCCTTCTTTGGTGGAACCATTCTGAGCAAGTCAGAAGCTGAAGGACCCAAAGAAGCATTCCTCAATTGGGAGCTCATTGACAGGTTTTGGAGGCTATCGATACCAGTGGGTGAGAACACTGATCACCCACTTGTGAACCCGTTCGGGCCATTTAGTCGGAGCCTTGAAGAAGTGCCGTTTGATCCGATCTTAGTGGTAGTTGGGGGAAGTGATCTCTTGAAAGACAGAGCTGCGGACTATGCAAATAGGCTGAAAAATTGGGGGAACAAGATTGAGTATGTCGAATTTGAAGGAAAGCAACACGGCTTCTTCACTATAGATCCTGATTCCGAAGCTTCGAAAGCACTCATGCTAATTGTCAAACGCTTCATTACTGAAAATTCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

329

Amino Acids

36.06

Weight (kDa)

5.21

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 62 - 121 4.9e-06 Carboxylesterase family
BD-FAE PF20434 66 - 178 7.6e-14 BD-FAE
Abhydrolase_3 PF07859 81 - 303 7.7e-60 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 128, 146
AatII GACGTC 1 cut(s) 126
AccII CGCG 1 cut(s) 279
AciI CCGC 9 cut(s) 196, 212, 255, 355, 373, 405, 507, 530, 830
AclWI GGATC 2 cut(s) 773, 911
AcoI YGGCCR 1 cut(s) 501
AcsI RAATTY 2 cut(s) 881, 976
AcuI CTGAAG 2 cut(s) 173, 657
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 2 cut(s) 34, 80
AfiI CCNNNNNNNGG 5 cut(s) 153, 379, 414, 605, 664
AflIII ACRYGT 1 cut(s) 480
AgsI TTSAA 6 cut(s) 422, 536, 764, 817, 887, 981
AjiI CACGTC 1 cut(s) 463
AjnI CCWGG 3 cut(s) 152, 281, 357
AjuI GAANNNNNNNTTGG 4 cut(s) 101, 133, 638, 670
AleI CACNNNNGTG 1 cut(s) 255
AloI GAACNNNNNNTCC 4 cut(s) 152, 184, 360, 392
Alw21I GWGCWC 2 cut(s) 591, 672
Alw26I GTCTC 1 cut(s) 346
AlwI GGATC 2 cut(s) 773, 911
AlwNI CAGNNNCTG 1 cut(s) 635
Ama87I CYCGRG 1 cut(s) 539
AoxI GGCC 4 cut(s) 284, 501, 543, 743
ApeKI GCWGC 2 cut(s) 383, 827
ApoI RAATTY 2 cut(s) 881, 976
ArsI GACNNNNNNTTYG 4 cut(s) 152, 184, 383, 415
Asp700I GAANNNNTTC 1 cut(s) 737
AspLEI GCGC 1 cut(s) 281
AspS9I GGNCC 4 cut(s) 285, 543, 641, 743
AsuC2I CCSGG 2 cut(s) 62, 380
AsuHPI GGTGA 3 cut(s) 506, 713, 719
AsuII TTCGAA 1 cut(s) 935
AvaI CYCGRG 1 cut(s) 539
AvaII GGWCC 1 cut(s) 641
BanII GRGCYC 1 cut(s) 672
Bbv12I GWGCWC 2 cut(s) 591, 672
BbvI GCAGC 2 cut(s) 370, 814
BccI CCATC 1 cut(s) 101
BceAI ACGGC 6 cut(s) 103, 157, 268, 392, 757, 916
BciT130I CCWGG 3 cut(s) 154, 283, 359
BclI TGATCA 1 cut(s) 718
BcnI CCSGG 2 cut(s) 62, 380
BcoDI GTCTC 1 cut(s) 346
BfaI CTAG 1 cut(s) 36
BfmI CTRYAG 1 cut(s) 912
BisI GCNGC 5 cut(s) 213, 384, 405, 508, 828
BlsI GCNGC 5 cut(s) 214, 385, 406, 509, 829
BmcAI AGTACT 1 cut(s) 34
Bme1390I CCNGG 5 cut(s) 62, 154, 283, 359, 380
Bme18I GGWCC 1 cut(s) 641
BmeT110I CYCGRG 1 cut(s) 539
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 4 cut(s) 285, 543, 641, 743
BmiI GGNNCC 9 cut(s) 91, 145, 363, 544, 564, 597, 613, 643, 758
BmrFI CCNGG 5 cut(s) 62, 154, 283, 359, 380
Bpu10I CCTNAGC 1 cut(s) 555
Bpu14I TTCGAA 1 cut(s) 935
BpuMI CCSGG 2 cut(s) 62, 380
Bsa29I ATCGAT 1 cut(s) 695
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 2 cut(s) 61, 250
BsaWI WCCGGW 1 cut(s) 565
BsaXI ACNNNNNCTCC 4 cut(s) 128, 158, 360, 390
Bsc4I CCNNNNNNNGG 5 cut(s) 153, 379, 414, 605, 664
Bse118I RCCGGY 3 cut(s) 208, 503, 545
Bse1I ACTGG 2 cut(s) 27, 701
Bse3DI GCAATG 1 cut(s) 513
BseBI CCWGG 3 cut(s) 154, 283, 359
BseCI ATCGAT 1 cut(s) 695
BseDI CCNNGG 2 cut(s) 61, 250
BseGI GGATG 1 cut(s) 246
BseLI CCNNNNNNNGG 5 cut(s) 153, 379, 414, 605, 664
BseMI GCAATG 1 cut(s) 513
BseMII CTCAG 3 cut(s) 334, 546, 611
BseNI ACTGG 2 cut(s) 27, 701
BseX3I CGGCCG 1 cut(s) 501
BseXI GCAGC 2 cut(s) 370, 814
Bsh1236I CGCG 1 cut(s) 279
Bsh1285I CGRYCG 1 cut(s) 504
BshFI GGCC 4 cut(s) 286, 503, 545, 745
BshVI ATCGAT 1 cut(s) 695
BsiEI CGRYCG 1 cut(s) 504
BsiHKAI GWGCWC 2 cut(s) 591, 672
BsiHKCI CYCGRG 1 cut(s) 539
BsiSI CCGG 7 cut(s) 61, 209, 347, 380, 504, 546, 566
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 5 cut(s) 153, 379, 414, 605, 664
BsmAI GTCTC 1 cut(s) 346
BsmFI GGGAC 1 cut(s) 110
BsmI GAATGC 2 cut(s) 62, 653
BsnI GGCC 4 cut(s) 286, 503, 545, 745
BsoBI CYCGRG 1 cut(s) 539
Bsp119I TTCGAA 1 cut(s) 935
Bsp1286I GDGCHC 2 cut(s) 591, 672
Bsp1407I TGTACA 1 cut(s) 78
Bsp143I GATC 5 cut(s) 718, 778, 783, 808, 916
BspACI CCGC 9 cut(s) 196, 212, 255, 355, 373, 405, 507, 530, 830
BspANI GGCC 4 cut(s) 286, 503, 545, 745
BspCNI CTCAG 3 cut(s) 333, 547, 612
BspDI ATCGAT 1 cut(s) 695
BspFNI CGCG 1 cut(s) 279
BspLI GGNNCC 9 cut(s) 91, 145, 363, 544, 564, 597, 613, 643, 758
BspPI GGATC 2 cut(s) 773, 911
BspT104I TTCGAA 1 cut(s) 935
BsrDI GCAATG 1 cut(s) 513
BsrFI RCCGGY 3 cut(s) 208, 503, 545
BsrGI TGTACA 1 cut(s) 78
BsrI ACTGG 2 cut(s) 27, 701
BssAI RCCGGY 3 cut(s) 208, 503, 545
BssECI CCNNGG 2 cut(s) 61, 250
BssMI GATC 5 cut(s) 718, 778, 783, 808, 916
BssNI GRCGYC 1 cut(s) 123
Bst2UI CCWGG 3 cut(s) 154, 283, 359
Bst4CI ACNGT 2 cut(s) 305, 398
BstACI GRCGYC 1 cut(s) 123
BstAUI TGTACA 1 cut(s) 78
BstBI TTCGAA 1 cut(s) 935
BstC8I GCNNGC 4 cut(s) 210, 315, 505, 561
BstDEI CTNAG 4 cut(s) 320, 555, 620, 787
BstDSI CCRYGG 1 cut(s) 250
BstF5I GGATG 1 cut(s) 246
BstFNI CGCG 1 cut(s) 279
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 5 cut(s) 721, 781, 786, 811, 919
BstMAI GTCTC 1 cut(s) 346
BstMBI GATC 5 cut(s) 718, 778, 783, 808, 916
BstMCI CGRYCG 1 cut(s) 504
BstMWI GCNNNNNNNGC 2 cut(s) 836, 938
BstNI CCWGG 3 cut(s) 154, 283, 359
BstSCI CCNGG 5 cut(s) 60, 152, 281, 357, 378
BstSFI CTRYAG 1 cut(s) 912
BstUI CGCG 1 cut(s) 279
BstV1I GCAGC 2 cut(s) 370, 814
BstX2I RGATCY 1 cut(s) 916
BstXI CCANNNNNNTGG 1 cut(s) 257
BstYI RGATCY 1 cut(s) 916
BstZI CGGCCG 1 cut(s) 501
Bsu15I ATCGAT 1 cut(s) 695
BsuRI GGCC 4 cut(s) 286, 503, 545, 745
BsuTUI ATCGAT 1 cut(s) 695
BtgI CCRYGG 1 cut(s) 250
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 1 cut(s) 246
BtsIMutI CAGTG 2 cut(s) 708, 714
Cac8I GCNNGC 4 cut(s) 210, 315, 505, 561
CaiI CAGNNNCTG 1 cut(s) 635
CfoI GCGC 1 cut(s) 281
Cfr10I RCCGGY 3 cut(s) 208, 503, 545
Cfr13I GGNCC 4 cut(s) 285, 543, 641, 743
ClaI ATCGAT 1 cut(s) 695
Csp6I GTAC 2 cut(s) 33, 79
CviAII CATG 1 cut(s) 946
CviQI GTAC 2 cut(s) 33, 79
DdeI CTNAG 4 cut(s) 320, 555, 620, 787
DpnI GATC 5 cut(s) 720, 780, 785, 810, 918
DpnII GATC 5 cut(s) 718, 778, 783, 808, 916
DrdI GACNNNNNNGTC 2 cut(s) 128, 146
DseDI GACNNNNNNGTC 2 cut(s) 128, 146
EaeI YGGCCR 1 cut(s) 501
EagI CGGCCG 1 cut(s) 501
EciI GGCGGA 1 cut(s) 185
Ecl136II GAGCTC 1 cut(s) 670
EclXI CGGCCG 1 cut(s) 501
Eco24I GRGCYC 1 cut(s) 672
Eco47I GGWCC 1 cut(s) 641
Eco52I CGGCCG 1 cut(s) 501
Eco53kI GAGCTC 1 cut(s) 670
Eco57I CTGAAG 2 cut(s) 173, 657
Eco88I CYCGRG 1 cut(s) 539
EcoICRI GAGCTC 1 cut(s) 670
EcoO109I RGGNCCY 1 cut(s) 641
EcoRII CCWGG 3 cut(s) 152, 281, 357
EcoT38I GRGCYC 1 cut(s) 672
FaeI CATG 1 cut(s) 949
FaiI YATR 7 cut(s) 269, 522, 585, 837, 876, 914, 947
FalI AAGNNNNNCTT 2 cut(s) 101, 133
FaqI GGGAC 1 cut(s) 110
FatI CATG 1 cut(s) 945
FbaI TGATCA 1 cut(s) 718
Fnu4HI GCNGC 5 cut(s) 213, 384, 405, 508, 828
FokI GGATG 1 cut(s) 233
FriOI GRGCYC 1 cut(s) 672
Fsp4HI GCNGC 5 cut(s) 213, 384, 405, 508, 828
FspBI CTAG 1 cut(s) 36
GlaI GCGC 1 cut(s) 280
GluI GCNGC 5 cut(s) 213, 384, 405, 508, 828
HaeIII GGCC 4 cut(s) 286, 503, 545, 745
HapII CCGG 7 cut(s) 61, 209, 347, 380, 504, 546, 566
HhaI GCGC 1 cut(s) 281
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 1 cut(s) 949
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HincII GTYRAC 1 cut(s) 456
HindII GTYRAC 1 cut(s) 456
HindIII AAGCTT 2 cut(s) 115, 930
HinfI GANTC 2 cut(s) 497, 923
HpaII CCGG 7 cut(s) 61, 209, 347, 380, 504, 546, 566
HphI GGTGA 3 cut(s) 506, 713, 719
Hpy166II GTNNAC 5 cut(s) 79, 133, 275, 456, 733
Hpy188I TCNGA 6 cut(s) 442, 621, 631, 756, 783, 928
Hpy188III TCNNGA 3 cut(s) 365, 814, 920
Hpy8I GTNNAC 5 cut(s) 79, 133, 275, 456, 733
Hpy99I CGWCG 7 cut(s) 89, 125, 140, 143, 152, 398, 442
HpyAV CCTTC 4 cut(s) 151, 610, 632, 881
HpyCH4III ACNGT 2 cut(s) 305, 398
HpyCH4IV ACGT 4 cut(s) 123, 162, 462, 480
HpyCH4V TGCA 2 cut(s) 267, 839
HpyF10VI GCNNNNNNNGC 2 cut(s) 836, 938
HpyF3I CTNAG 4 cut(s) 320, 555, 620, 787
HpySE526I ACGT 4 cut(s) 123, 162, 462, 480
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 1 cut(s) 949
HspAI GCGC 1 cut(s) 279
KroI GCCGGC 2 cut(s) 208, 503
KroNI GCCGGC 2 cut(s) 210, 505
Ksp22I TGATCA 1 cut(s) 718
Kzo9I GATC 5 cut(s) 718, 778, 783, 808, 916
LmnI GCTCC 8 cut(s) 95, 149, 198, 367, 568, 601, 667, 756
Lsp1109I GCAGC 2 cut(s) 370, 814
MaeI CTAG 1 cut(s) 36
MaeII ACGT 4 cut(s) 123, 162, 462, 480
MaeIII GTNAC 2 cut(s) 476, 494
MalI GATC 5 cut(s) 720, 780, 785, 810, 918
MboI GATC 5 cut(s) 718, 778, 783, 808, 916
MboII GAAGA 4 cut(s) 62, 65, 776, 898
MfeI CAATTG 1 cut(s) 661
MflI RGATCY 1 cut(s) 916
MhlI GDGCHC 2 cut(s) 591, 672
MluCI AATT 6 cut(s) 523, 661, 853, 881, 951, 976
MlyI GAGTC 1 cut(s) 491
MmeI TCCRAC 2 cut(s) 420, 734
MnlI CCTC 7 cut(s) 115, 209, 335, 386, 436, 668, 681
MroNI GCCGGC 2 cut(s) 208, 503
MroXI GAANNNNTTC 1 cut(s) 737
MseI TTAA 1 cut(s) 411
MslI CAYNNNNRTG 1 cut(s) 255
MspI CCGG 7 cut(s) 61, 209, 347, 380, 504, 546, 566
MspR9I CCNGG 5 cut(s) 62, 154, 283, 359, 380
MunI CAATTG 1 cut(s) 661
Mva1269I GAATGC 2 cut(s) 62, 653
MvaI CCWGG 3 cut(s) 154, 283, 359
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 2 cut(s) 836, 938
NaeI GCCGGC 2 cut(s) 210, 505
NciI CCSGG 2 cut(s) 62, 380
NdeII GATC 5 cut(s) 718, 778, 783, 808, 916
NgoMIV GCCGGC 2 cut(s) 208, 503
NlaIII CATG 1 cut(s) 949
NlaIV GGNNCC 9 cut(s) 91, 145, 363, 544, 564, 597, 613, 643, 758
NmeAIII GCCGAG 1 cut(s) 479
NmuCI GTSAC 1 cut(s) 494
NspV TTCGAA 1 cut(s) 935
OliI CACNNNNGTG 1 cut(s) 255
PcsI WCGNNNNNNNCGW 3 cut(s) 93, 144, 168
PctI GAATGC 2 cut(s) 62, 653
PdiI GCCGGC 2 cut(s) 210, 505
PdmI GAANNNNTTC 1 cut(s) 737
PfeI GAWTC 1 cut(s) 923
PkrI GCNGC 5 cut(s) 214, 385, 406, 509, 829
PleI GAGTC 1 cut(s) 491
PpsI GAGTC 1 cut(s) 491
PpuMI RGGWCCY 1 cut(s) 641
Psp124BI GAGCTC 1 cut(s) 672
Psp5II RGGWCCY 1 cut(s) 641
Psp6I CCWGG 3 cut(s) 152, 281, 357
PspGI CCWGG 3 cut(s) 152, 281, 357
PspN4I GGNNCC 9 cut(s) 91, 145, 363, 544, 564, 597, 613, 643, 758
PspPI GGNCC 4 cut(s) 285, 543, 641, 743
PspPPI RGGWCCY 1 cut(s) 641
PstNI CAGNNNCTG 1 cut(s) 635
PsuI RGATCY 1 cut(s) 916
RsaI GTAC 2 cut(s) 34, 80
RsaNI GTAC 2 cut(s) 33, 79
RseI CAYNNNNRTG 1 cut(s) 255
SacI GAGCTC 1 cut(s) 672
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 5 cut(s) 213, 384, 405, 508, 828
Sau3AI GATC 5 cut(s) 718, 778, 783, 808, 916
Sau96I GGNCC 4 cut(s) 285, 543, 641, 743
ScaI AGTACT 1 cut(s) 34
SchI GAGTC 1 cut(s) 491
ScrFI CCNGG 5 cut(s) 62, 154, 283, 359, 380
SduI GDGCHC 2 cut(s) 591, 672
SfcI CTRYAG 1 cut(s) 912
SfuI TTCGAA 1 cut(s) 935
SinI GGWCC 1 cut(s) 641
SmiMI CAYNNNNRTG 1 cut(s) 255
Sse9I AATT 6 cut(s) 523, 661, 853, 881, 951, 976
SsiI CCGC 9 cut(s) 196, 212, 255, 355, 373, 405, 507, 530, 830
SspI AATATT 1 cut(s) 237
SspMI CTAG 1 cut(s) 36
SstI GAGCTC 1 cut(s) 672
StyD4I CCNGG 5 cut(s) 60, 152, 281, 357, 378
TaaI ACNGT 2 cut(s) 305, 398
TaiI ACGT 4 cut(s) 126, 165, 465, 483
TaqI TCGA 5 cut(s) 120, 171, 695, 879, 935
TasI AATT 6 cut(s) 523, 661, 853, 881, 951, 976
TatI WGTACW 2 cut(s) 32, 78
TauI GCSGC 3 cut(s) 215, 407, 510
TfiI GAWTC 1 cut(s) 923
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 2 cut(s) 708, 721
TseFI GTSAC 1 cut(s) 494
TseI GCWGC 2 cut(s) 383, 827
Tsp45I GTSAC 1 cut(s) 494
TspDTI ATGAA 2 cut(s) 475, 955
TspGWI ACGGA 2 cut(s) 89, 136
TspRI CASTG 2 cut(s) 708, 721
VpaK11BI GGWCC 1 cut(s) 641
XapI RAATTY 2 cut(s) 881, 976
XmnI GAANNNNTTC 1 cut(s) 737
XspI CTAG 1 cut(s) 36
ZraI GACGTC 1 cut(s) 124
ZrmI AGTACT 1 cut(s) 34
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.