Rw5G030600

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001599
Physical Location & Seq
Reverse (-)
48629739 .. 48630914
1176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G030600.1

Sequence Viewer

Length: 972 bp
ATGTCGAACACTACTGAAACTACTTTACCCTACGAAGAATGCCGGGGCGTTCTCCGCGTCTACAGCAATGGCTCAATCGTCCGTTCCCCCAAGCCAAGCTTCGACGTCCCTGTCCACGACGACGGCTCCGTCGCGTGGAAGGACGTAGTTTTCGACCACACTCACCAGCTTCAGCTCCGCCTCTACAAGCCGGCGGCTGCAAAATCCAAGCTACCAATATTCTACTACATCCACGGCGGCGGCTTCTGCATCGGCTCACGCGCTTGGCCCAACTGCCAAAACTACTGCTTCCAGCTTGCTCTGAGCCTCCAAGCCGTCATAGTCTCGCCAGACTATAGGCTGGCTCCCGAACACCGGCTCCCGGCTGCCATTGACGACGGCTTCACTGCTGTCAAGTGGCTCCAAACCCAAGCCGTGTCGGAGGAGCCCGACACGTGGCTGACTGACGTGGCTGACTTTGGCAACGTCTTCATTTCTGGTGACTCGGCTGGCGGCAACATTGCTCACAATTTGGCCGTTCAAATCGGGGCCGGTTCACCTGAGCTGGCTCCGGTTCAGGTCAAGGGTTATGTTTTTTTGGCTCCTTTCTTTGGTGGAACCATTCTTAGCAAGTCAGAAGCTGAAGGACCCAAAGAAGCATTCCTCAATTGGGAGCTCATTGACAGGTTTTGGAGACTATCGATACCAATTGGAGAGACCACTGATCACCCACTTGTGAACCCGTTCGGGCCAAATAGCCGGAGCCTTGAAGAAGTACCCTTTGATCCGATCTTGGTGGTAGTTGGGGGAAGTGATCTCTTGAAAGATAGAGCTGAGGACTATGCAAAAAGGCTAAAAGCTTGGGGGAACAAGATTGAGTATGTCGAATTTGAAGGAAAGCAACATGGCTTCTTCACTATTGATCCTAATTCGGAAGCTTCAAAAGAACTCATGCTAATTGTCAAACGGTTCATTACTGAAAATTCCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

323

Amino Acids

35.79

Weight (kDa)

5.23

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 57 - 115 9.9e-06 Carboxylesterase family
BD-FAE PF20434 60 - 173 2.2e-13 BD-FAE
Abhydrolase_3 PF07859 75 - 298 1.2e-59 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 110, 128
AatII GACGTC 1 cut(s) 108
AccI GTMKAC 1 cut(s) 60
AccII CGCG 3 cut(s) 57, 134, 261
AciI CCGC 6 cut(s) 55, 178, 194, 237, 240, 492
AclWI GGATC 2 cut(s) 758, 896
AcoI YGGCCR 1 cut(s) 513
AcsI RAATTY 2 cut(s) 866, 961
AcuI CTGAAG 2 cut(s) 155, 642
AcvI CACGTG 1 cut(s) 435
AcyI GRCGYC 1 cut(s) 105
AfaI GTAC 1 cut(s) 756
AfiI CCNNNNNNNGG 6 cut(s) 135, 354, 361, 435, 590, 649
AflIII ACRYGT 1 cut(s) 432
AgsI TTSAA 5 cut(s) 521, 749, 802, 872, 921
AjiI CACGTC 1 cut(s) 448
AjuI GAANNNNNNNTTGG 4 cut(s) 83, 115, 623, 655
AloI GAACNNNNNNTCC 2 cut(s) 342, 374
Alw21I GWGCWC 1 cut(s) 657
Alw26I GTCTC 3 cut(s) 328, 667, 689
AlwI GGATC 2 cut(s) 758, 896
AlwNI CAGNNNCTG 1 cut(s) 620
AoxI GGCC 4 cut(s) 266, 513, 528, 728
ApeKI GCWGC 2 cut(s) 197, 365
ApoI RAATTY 2 cut(s) 866, 961
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
Asp700I GAANNNNTTC 1 cut(s) 722
AspLEI GCGC 1 cut(s) 263
AspS9I GGNCC 4 cut(s) 267, 528, 626, 728
AsuC2I CCSGG 2 cut(s) 44, 362
AsuHPI GGTGA 4 cut(s) 155, 491, 528, 698
AvaII GGWCC 1 cut(s) 626
BanII GRGCYC 2 cut(s) 429, 657
BbrPI CACGTG 1 cut(s) 435
BbsI GAAGAC 1 cut(s) 460
Bbv12I GWGCWC 1 cut(s) 657
BbvCI CCTCAGC 1 cut(s) 813
BbvI GCAGC 2 cut(s) 184, 352
BceAI ACGGC 6 cut(s) 139, 250, 299, 394, 398, 500
BclI TGATCA 1 cut(s) 703
BcnI CCSGG 2 cut(s) 44, 362
BcoDI GTCTC 3 cut(s) 328, 667, 689
BfmI CTRYAG 2 cut(s) 61, 334
BisI GCNGC 6 cut(s) 195, 198, 238, 241, 366, 493
BlsI GCNGC 6 cut(s) 196, 199, 239, 242, 367, 494
Bme1390I CCNGG 2 cut(s) 44, 362
Bme18I GGWCC 1 cut(s) 626
BmgBI CACGTC 1 cut(s) 448
BmgT120I GGNCC 4 cut(s) 267, 528, 626, 728
BmrFI CCNGG 2 cut(s) 44, 362
BmsI GCATC 1 cut(s) 258
BpiI GAAGAC 1 cut(s) 460
Bpu10I CCTNAGC 2 cut(s) 540, 813
BpuMI CCSGG 2 cut(s) 44, 362
Bsa29I ATCGAT 1 cut(s) 680
BsaAI YACGTR 1 cut(s) 435
BsaHI GRCGYC 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 689
BsaJI CCNNGG 2 cut(s) 43, 232
BsaWI WCCGGW 1 cut(s) 550
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 342, 372
Bsc4I CCNNNNNNNGG 6 cut(s) 135, 354, 361, 435, 590, 649
Bse118I RCCGGY 3 cut(s) 190, 354, 530
Bse3DI GCAATG 2 cut(s) 73, 498
BseCI ATCGAT 1 cut(s) 680
BseDI CCNNGG 2 cut(s) 43, 232
BseGI GGATG 1 cut(s) 228
BseLI CCNNNNNNNGG 6 cut(s) 135, 354, 361, 435, 590, 649
BseMI GCAATG 2 cut(s) 73, 498
BseMII CTCAG 3 cut(s) 293, 531, 804
BseRI GAGGAG 1 cut(s) 437
BseXI GCAGC 2 cut(s) 184, 352
Bsh1236I CGCG 3 cut(s) 57, 134, 261
BshFI GGCC 4 cut(s) 268, 515, 530, 730
BshVI ATCGAT 1 cut(s) 680
BsiHKAI GWGCWC 1 cut(s) 657
BsiSI CCGG 7 cut(s) 43, 191, 355, 362, 531, 551, 739
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 6 cut(s) 135, 354, 361, 435, 590, 649
BsmAI GTCTC 3 cut(s) 328, 667, 689
BsmFI GGGAC 1 cut(s) 92
BsmI GAATGC 2 cut(s) 44, 638
BsnI GGCC 4 cut(s) 268, 515, 530, 730
Bso31I GGTCTC 1 cut(s) 689
Bsp1286I GDGCHC 2 cut(s) 429, 657
Bsp143I GATC 5 cut(s) 703, 763, 768, 793, 901
BspACI CCGC 6 cut(s) 55, 178, 194, 237, 240, 492
BspANI GGCC 4 cut(s) 268, 515, 530, 730
BspCNI CTCAG 3 cut(s) 294, 532, 805
BspDI ATCGAT 1 cut(s) 680
BspFNI CGCG 3 cut(s) 57, 134, 261
BspPI GGATC 2 cut(s) 758, 896
BspTNI GGTCTC 1 cut(s) 689
BsrDI GCAATG 2 cut(s) 73, 498
BsrFI RCCGGY 3 cut(s) 190, 354, 530
BssAI RCCGGY 3 cut(s) 190, 354, 530
BssECI CCNNGG 2 cut(s) 43, 232
BssMI GATC 5 cut(s) 703, 763, 768, 793, 901
BssNI GRCGYC 1 cut(s) 105
Bst4CI ACNGT 1 cut(s) 948
BstACI GRCGYC 1 cut(s) 105
BstBAI YACGTR 1 cut(s) 435
BstC8I GCNNGC 5 cut(s) 192, 297, 342, 490, 546
BstDEI CTNAG 4 cut(s) 302, 540, 605, 813
BstDSI CCRYGG 1 cut(s) 232
BstF5I GGATG 1 cut(s) 228
BstFNI CGCG 3 cut(s) 57, 134, 261
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 5 cut(s) 706, 766, 771, 796, 904
BstMAI GTCTC 3 cut(s) 328, 667, 689
BstMBI GATC 5 cut(s) 703, 763, 768, 793, 901
BstMWI GCNNNNNNNGC 3 cut(s) 54, 63, 246
BstSCI CCNGG 2 cut(s) 42, 360
BstSFI CTRYAG 2 cut(s) 61, 334
BstUI CGCG 3 cut(s) 57, 134, 261
BstV1I GCAGC 2 cut(s) 184, 352
BstV2I GAAGAC 1 cut(s) 460
Bsu15I ATCGAT 1 cut(s) 680
BsuRI GGCC 4 cut(s) 268, 515, 530, 730
BsuTUI ATCGAT 1 cut(s) 680
BtgI CCRYGG 1 cut(s) 232
BtrI CACGTC 1 cut(s) 448
BtsCI GGATG 1 cut(s) 228
BtsI GCAGTG 1 cut(s) 384
BtsIMutI CAGTG 2 cut(s) 384, 699
Cac8I GCNNGC 5 cut(s) 192, 297, 342, 490, 546
CaiI CAGNNNCTG 1 cut(s) 620
CfoI GCGC 1 cut(s) 263
Cfr10I RCCGGY 3 cut(s) 190, 354, 530
Cfr13I GGNCC 4 cut(s) 267, 528, 626, 728
ClaI ATCGAT 1 cut(s) 680
CseI GACGC 1 cut(s) 46
Csp6I GTAC 1 cut(s) 755
CviAII CATG 2 cut(s) 884, 931
CviQI GTAC 1 cut(s) 755
DdeI CTNAG 4 cut(s) 302, 540, 605, 813
DpnI GATC 5 cut(s) 705, 765, 770, 795, 903
DpnII GATC 5 cut(s) 703, 763, 768, 793, 901
DrdI GACNNNNNNGTC 2 cut(s) 110, 128
DseDI GACNNNNNNGTC 2 cut(s) 110, 128
EaeI YGGCCR 1 cut(s) 513
EciI GGCGGA 1 cut(s) 167
Ecl136II GAGCTC 1 cut(s) 655
Eco24I GRGCYC 2 cut(s) 429, 657
Eco31I GGTCTC 1 cut(s) 689
Eco47I GGWCC 1 cut(s) 626
Eco53kI GAGCTC 1 cut(s) 655
Eco57I CTGAAG 2 cut(s) 155, 642
Eco72I CACGTG 1 cut(s) 435
EcoICRI GAGCTC 1 cut(s) 655
EcoO109I RGGNCCY 1 cut(s) 626
EcoT38I GRGCYC 2 cut(s) 429, 657
FaeI CATG 2 cut(s) 887, 934
FaiI YATR 7 cut(s) 320, 336, 570, 822, 861, 885, 932
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FaqI GGGAC 1 cut(s) 92
FatI CATG 2 cut(s) 883, 930
FbaI TGATCA 1 cut(s) 703
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 6 cut(s) 195, 198, 238, 241, 366, 493
FokI GGATG 1 cut(s) 215
FriOI GRGCYC 2 cut(s) 429, 657
Fsp4HI GCNGC 6 cut(s) 195, 198, 238, 241, 366, 493
GlaI GCGC 1 cut(s) 262
GluI GCNGC 6 cut(s) 195, 198, 238, 241, 366, 493
HaeIII GGCC 4 cut(s) 268, 515, 530, 730
HapII CCGG 7 cut(s) 43, 191, 355, 362, 531, 551, 739
HgaI GACGC 1 cut(s) 46
HhaI GCGC 1 cut(s) 263
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 2 cut(s) 887, 934
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HindIII AAGCTT 3 cut(s) 97, 837, 915
HinfI GANTC 1 cut(s) 482
HpaII CCGG 7 cut(s) 43, 191, 355, 362, 531, 551, 739
HphI GGTGA 4 cut(s) 155, 491, 528, 698
Hpy166II GTNNAC 4 cut(s) 61, 115, 536, 718
Hpy188I TCNGA 5 cut(s) 303, 421, 616, 768, 913
Hpy188III TCNNGA 2 cut(s) 347, 799
Hpy8I GTNNAC 4 cut(s) 61, 115, 536, 718
Hpy99I CGWCG 5 cut(s) 107, 122, 125, 134, 380
HpyAV CCTTC 3 cut(s) 133, 617, 866
HpyCH4III ACNGT 1 cut(s) 948
HpyCH4IV ACGT 5 cut(s) 105, 144, 434, 447, 465
HpyCH4V TGCA 3 cut(s) 200, 249, 824
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 63, 246
HpyF3I CTNAG 4 cut(s) 302, 540, 605, 813
HpySE526I ACGT 5 cut(s) 105, 144, 434, 447, 465
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 2 cut(s) 887, 934
HspAI GCGC 1 cut(s) 261
KroI GCCGGC 1 cut(s) 190
KroNI GCCGGC 1 cut(s) 192
Ksp22I TGATCA 1 cut(s) 703
Kzo9I GATC 5 cut(s) 703, 763, 768, 793, 901
Lsp1109I GCAGC 2 cut(s) 184, 352
LweI GCATC 1 cut(s) 258
MaeII ACGT 5 cut(s) 105, 144, 434, 447, 465
MaeIII GTNAC 1 cut(s) 479
MalI GATC 5 cut(s) 705, 765, 770, 795, 903
MboI GATC 5 cut(s) 703, 763, 768, 793, 901
MboII GAAGA 4 cut(s) 47, 460, 761, 883
MfeI CAATTG 2 cut(s) 646, 687
MhlI GDGCHC 2 cut(s) 429, 657
MluCI AATT 7 cut(s) 508, 646, 687, 866, 907, 936, 961
MlyI GAGTC 1 cut(s) 476
MmeI TCCRAC 1 cut(s) 399
MnlI CCTC 5 cut(s) 191, 317, 415, 653, 808
MroNI GCCGGC 1 cut(s) 190
MroXI GAANNNNTTC 1 cut(s) 722
MspA1I CMGCKG 1 cut(s) 969
MspI CCGG 7 cut(s) 43, 191, 355, 362, 531, 551, 739
MspR9I CCNGG 2 cut(s) 44, 362
MunI CAATTG 2 cut(s) 646, 687
Mva1269I GAATGC 2 cut(s) 44, 638
MvnI CGCG 3 cut(s) 57, 134, 261
MwoI GCNNNNNNNGC 3 cut(s) 54, 63, 246
NaeI GCCGGC 1 cut(s) 192
NciI CCSGG 2 cut(s) 44, 362
NdeII GATC 5 cut(s) 703, 763, 768, 793, 901
NgoMIV GCCGGC 1 cut(s) 190
NlaIII CATG 2 cut(s) 887, 934
NmeAIII GCCGAG 1 cut(s) 464
NmuCI GTSAC 1 cut(s) 479
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 150
PctI GAATGC 2 cut(s) 44, 638
PdiI GCCGGC 1 cut(s) 192
PdmI GAANNNNTTC 1 cut(s) 722
PkrI GCNGC 6 cut(s) 196, 199, 239, 242, 367, 494
PleI GAGTC 1 cut(s) 476
PmaCI CACGTG 1 cut(s) 435
PmlI CACGTG 1 cut(s) 435
PpsI GAGTC 1 cut(s) 476
Ppu21I YACGTR 1 cut(s) 435
PpuMI RGGWCCY 1 cut(s) 626
Psp124BI GAGCTC 1 cut(s) 657
Psp5II RGGWCCY 1 cut(s) 626
PspCI CACGTG 1 cut(s) 435
PspPI GGNCC 4 cut(s) 267, 528, 626, 728
PspPPI RGGWCCY 1 cut(s) 626
PstNI CAGNNNCTG 1 cut(s) 620
PvuII CAGCTG 1 cut(s) 969
RsaI GTAC 1 cut(s) 756
RsaNI GTAC 1 cut(s) 755
SacI GAGCTC 1 cut(s) 657
SatI GCNGC 6 cut(s) 195, 198, 238, 241, 366, 493
Sau3AI GATC 5 cut(s) 703, 763, 768, 793, 901
Sau96I GGNCC 4 cut(s) 267, 528, 626, 728
SchI GAGTC 1 cut(s) 476
ScrFI CCNGG 2 cut(s) 44, 362
SduI GDGCHC 2 cut(s) 429, 657
SfaNI GCATC 1 cut(s) 258
SfcI CTRYAG 2 cut(s) 61, 334
SinI GGWCC 1 cut(s) 626
Sse9I AATT 7 cut(s) 508, 646, 687, 866, 907, 936, 961
SsiI CCGC 6 cut(s) 55, 178, 194, 237, 240, 492
SspI AATATT 1 cut(s) 219
SstI GAGCTC 1 cut(s) 657
StyD4I CCNGG 2 cut(s) 42, 360
TaaI ACNGT 1 cut(s) 948
TaiI ACGT 5 cut(s) 108, 147, 437, 450, 468
TaqI TCGA 5 cut(s) 5, 102, 153, 680, 864
TasI AATT 7 cut(s) 508, 646, 687, 866, 907, 936, 961
TauI GCSGC 4 cut(s) 197, 240, 243, 495
TscAI CASTG 2 cut(s) 391, 706
TseFI GTSAC 1 cut(s) 479
TseI GCWGC 2 cut(s) 197, 365
Tsp45I GTSAC 1 cut(s) 479
TspDTI ATGAA 2 cut(s) 460, 940
TspGWI ACGGA 2 cut(s) 71, 118
TspRI CASTG 2 cut(s) 391, 706
VpaK11BI GGWCC 1 cut(s) 626
XapI RAATTY 2 cut(s) 866, 961
XmiI GTMKAC 1 cut(s) 60
XmnI GAANNNNTTC 1 cut(s) 722
ZraI GACGTC 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.