RLG00000034605

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001599
Physical Location & Seq
Reverse (-)
45920844 .. 45922661
1818 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034605

Sequence Viewer

Length: 1092 bp
ATGTCGAACACTGCTGAAACTACTTTACCCTACGAAGACTGCCGGGGCGTTCTTCGCGTCTATAGTGACGGCTCCATCGTCCGTTCCCCCAAGCCAAGCTTCGACGTCCCTGTCCACGACGACGGCTCCGTCGCGTGGAAGGACGTAGTTTTCGACCACACTCACCAGCTTCAGCTCCGCCTCTACAAGCCCGCGGCTGCAAAATCCAAGCTACCAATATTCTACTACATCCACGGCGGCGGCTTCTGCATCGGCTCACGCGCTTGGCCCAACTGCCAAAACTACTGCTTCCAGCTCGCTCTGAGCCTCCAAGCCGTCATAGTCTCGCCAGACTATCGGCTGGCCCCCGAACACCGGCTCCCGGCTGCCATTGACGACGGCTTCACTGCCGTCAAGTGGCTCCAAGCCCAAGCCGTGTCGGATGAGCCCGACACGTGGCTGACTGACGTGGCTGACTTTGGCAACGTCTTCATTTCTGGTGACTCGGCTGGCGGCAACATTGCTCACAATTTAGCGGTTCAAATCGGGGCCCGTTCACCTGAGCTGGCTCCGGTTCAGGTCAAGGGTTACGTTTTTTTGGCTCCTTTCTTTGGTGGAACCATTCTGAGCAAGTCAGAAGCTGAAGGACCCAAAGAAGCATTTCTCAATTGGGAGCTCATTGACAGGTTTTGGAGGCTATCGATACCAATCGGAGAGAACACTGATCACCCACTTGTGAACCCGTTCGGGCCAAATAGCCGGAGCCTTGAAGGAGTACCCTTTGATCCGATCTTGGTGGTAGTTGGGGGAAGTGATCTCTTGAAAGATAGAGCTGAGGACTACGCAAAAAGGCTAAAAGCTTGGGGGAACAAGATTGAGTATGTCGAATTTGAAGGAAAGCAACATGGCTTCTTCACTATTGATCCTAATTCGGAAGCTTCAAAAGAACTCATGCTAATTGTCAAACGTGTGGACTGTGATATGGTGGCGGCACGGGATGCAACGACTACAATGGCTTTTGACGACGAAGCGGGGGACGACGCAGATGGGGCTGCTCGTCATAATTGGATTCCCAGATCACCCTTGGATGTCCAAAGCAAATTTGGGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

364

Amino Acids

40.01

Weight (kDa)

4.97

Isoelectric Point (pI)

42.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 60 - 173 1.7e-13 BD-FAE
Abhydrolase_3 PF07859 75 - 298 2.9e-60 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 110, 128
AatII GACGTC 1 cut(s) 108
AccII CGCG 4 cut(s) 57, 134, 194, 261
AciI CCGC 9 cut(s) 178, 192, 194, 237, 240, 492, 515, 968, 1010
AclWI GGATC 2 cut(s) 758, 896
AcsI RAATTY 2 cut(s) 866, 1079
AcuI CTGAAG 2 cut(s) 155, 642
AcvI CACGTG 1 cut(s) 435
AcyI GRCGYC 1 cut(s) 105
AfaI GTAC 1 cut(s) 756
AfiI CCNNNNNNNGG 6 cut(s) 135, 354, 361, 396, 435, 590
AflIII ACRYGT 2 cut(s) 432, 946
AgsI TTSAA 5 cut(s) 521, 749, 802, 872, 921
AjiI CACGTC 1 cut(s) 448
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
AloI GAACNNNNNNTCC 2 cut(s) 342, 374
Alw21I GWGCWC 1 cut(s) 657
Alw26I GTCTC 1 cut(s) 328
AlwI GGATC 2 cut(s) 758, 896
AlwNI CAGNNNCTG 1 cut(s) 620
AoxI GGCC 4 cut(s) 266, 342, 528, 728
ApaI GGGCCC 1 cut(s) 532
ApeKI GCWGC 3 cut(s) 197, 365, 1031
ApoI RAATTY 2 cut(s) 866, 1079
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
Asp700I GAANNNNTTC 2 cut(s) 639, 722
AspLEI GCGC 1 cut(s) 263
AspS9I GGNCC 6 cut(s) 267, 343, 528, 529, 626, 728
AsuC2I CCSGG 2 cut(s) 44, 362
AsuHPI GGTGA 5 cut(s) 155, 491, 528, 698, 1050
AvaII GGWCC 1 cut(s) 626
BaeGI GKGCMC 1 cut(s) 532
BanII GRGCYC 3 cut(s) 429, 532, 657
BbrPI CACGTG 1 cut(s) 435
BbsI GAAGAC 2 cut(s) 42, 460
Bbv12I GWGCWC 1 cut(s) 657
BbvCI CCTCAGC 1 cut(s) 813
BbvI GCAGC 3 cut(s) 184, 352, 1018
BccI CCATC 2 cut(s) 83, 1019
BceAI ACGGC 7 cut(s) 85, 139, 250, 299, 374, 394, 398
BclI TGATCA 1 cut(s) 703
BcnI CCSGG 2 cut(s) 44, 362
BcoDI GTCTC 1 cut(s) 328
BfmI CTRYAG 1 cut(s) 61
BisI GCNGC 8 cut(s) 195, 198, 238, 241, 366, 493, 969, 1032
BlsI GCNGC 8 cut(s) 196, 199, 239, 242, 367, 494, 970, 1033
Bme1390I CCNGG 2 cut(s) 44, 362
Bme18I GGWCC 1 cut(s) 626
BmgBI CACGTC 1 cut(s) 448
BmgT120I GGNCC 6 cut(s) 267, 343, 528, 529, 626, 728
BmrFI CCNGG 2 cut(s) 44, 362
BmsI GCATC 2 cut(s) 258, 967
BpiI GAAGAC 2 cut(s) 42, 460
Bpu10I CCTNAGC 2 cut(s) 540, 813
BpuMI CCSGG 2 cut(s) 44, 362
Bsa29I ATCGAT 1 cut(s) 680
BsaAI YACGTR 1 cut(s) 435
BsaBI GATNNNNATC 1 cut(s) 686
BsaHI GRCGYC 1 cut(s) 105
BsaJI CCNNGG 4 cut(s) 43, 192, 232, 1062
BsaWI WCCGGW 1 cut(s) 550
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 342, 372
Bsc4I CCNNNNNNNGG 6 cut(s) 135, 354, 361, 396, 435, 590
Bse118I RCCGGY 1 cut(s) 354
Bse3DI GCAATG 1 cut(s) 498
Bse8I GATNNNNATC 1 cut(s) 686
BseCI ATCGAT 1 cut(s) 680
BseDI CCNNGG 4 cut(s) 43, 192, 232, 1062
BseGI GGATG 4 cut(s) 228, 427, 982, 1072
BseJI GATNNNNATC 1 cut(s) 686
BseLI CCNNNNNNNGG 6 cut(s) 135, 354, 361, 396, 435, 590
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 4 cut(s) 293, 531, 596, 804
BseSI GKGCMC 1 cut(s) 532
BseXI GCAGC 3 cut(s) 184, 352, 1018
Bsh1236I CGCG 4 cut(s) 57, 134, 194, 261
BshFI GGCC 4 cut(s) 268, 344, 530, 730
BshVI ATCGAT 1 cut(s) 680
BsiHKAI GWGCWC 1 cut(s) 657
BsiSI CCGG 5 cut(s) 43, 355, 362, 551, 739
BslFI GGGAC 2 cut(s) 92, 1028
BslI CCNNNNNNNGG 6 cut(s) 135, 354, 361, 396, 435, 590
BsmAI GTCTC 1 cut(s) 328
BsmFI GGGAC 2 cut(s) 92, 1028
BsnI GGCC 4 cut(s) 268, 344, 530, 730
Bsp120I GGGCCC 1 cut(s) 528
Bsp1286I GDGCHC 3 cut(s) 429, 532, 657
Bsp143I GATC 6 cut(s) 703, 763, 768, 793, 901, 1055
BspACI CCGC 9 cut(s) 178, 192, 194, 237, 240, 492, 515, 968, 1010
BspANI GGCC 4 cut(s) 268, 344, 530, 730
BspCNI CTCAG 4 cut(s) 294, 532, 597, 805
BspDI ATCGAT 1 cut(s) 680
BspFNI CGCG 4 cut(s) 57, 134, 194, 261
BspPI GGATC 2 cut(s) 758, 896
BsrDI GCAATG 1 cut(s) 498
BsrFI RCCGGY 1 cut(s) 354
BssAI RCCGGY 1 cut(s) 354
BssECI CCNNGG 4 cut(s) 43, 192, 232, 1062
BssMI GATC 6 cut(s) 703, 763, 768, 793, 901, 1055
BssNI GRCGYC 1 cut(s) 105
BssT1I CCWWGG 1 cut(s) 1062
Bst4CI ACNGT 1 cut(s) 956
BstACI GRCGYC 1 cut(s) 105
BstAPI GCANNNNNTGC 1 cut(s) 977
BstBAI YACGTR 1 cut(s) 435
BstC8I GCNNGC 5 cut(s) 192, 297, 342, 490, 546
BstDEI CTNAG 4 cut(s) 302, 540, 605, 813
BstDSI CCRYGG 2 cut(s) 192, 232
BstF5I GGATG 4 cut(s) 228, 427, 982, 1072
BstFNI CGCG 4 cut(s) 57, 134, 194, 261
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 6 cut(s) 706, 766, 771, 796, 904, 1058
BstMAI GTCTC 1 cut(s) 328
BstMBI GATC 6 cut(s) 703, 763, 768, 793, 901, 1055
BstMWI GCNNNNNNNGC 4 cut(s) 54, 246, 977, 1028
BstSCI CCNGG 2 cut(s) 42, 360
BstSFI CTRYAG 1 cut(s) 61
BstSLI GKGCMC 1 cut(s) 532
BstUI CGCG 4 cut(s) 57, 134, 194, 261
BstV1I GCAGC 3 cut(s) 184, 352, 1018
BstV2I GAAGAC 2 cut(s) 42, 460
Bsu15I ATCGAT 1 cut(s) 680
BsuRI GGCC 4 cut(s) 268, 344, 530, 730
BsuTUI ATCGAT 1 cut(s) 680
BtgI CCRYGG 2 cut(s) 192, 232
BtrI CACGTC 1 cut(s) 448
BtsCI GGATG 4 cut(s) 228, 427, 982, 1072
BtsI GCAGTG 2 cut(s) 9, 384
BtsIMutI CAGTG 3 cut(s) 9, 384, 699
Cac8I GCNNGC 5 cut(s) 192, 297, 342, 490, 546
CaiI CAGNNNCTG 1 cut(s) 620
CfoI GCGC 1 cut(s) 263
Cfr10I RCCGGY 1 cut(s) 354
Cfr13I GGNCC 6 cut(s) 267, 343, 528, 529, 626, 728
Cfr42I CCGCGG 1 cut(s) 195
ClaI ATCGAT 1 cut(s) 680
CseI GACGC 2 cut(s) 46, 1028
Csp6I GTAC 1 cut(s) 755
CviAII CATG 2 cut(s) 884, 931
CviQI GTAC 1 cut(s) 755
DdeI CTNAG 4 cut(s) 302, 540, 605, 813
DpnI GATC 6 cut(s) 705, 765, 770, 795, 903, 1057
DpnII GATC 6 cut(s) 703, 763, 768, 793, 901, 1055
DrdI GACNNNNNNGTC 2 cut(s) 110, 128
DseDI GACNNNNNNGTC 2 cut(s) 110, 128
EciI GGCGGA 1 cut(s) 167
Ecl136II GAGCTC 1 cut(s) 655
Eco130I CCWWGG 1 cut(s) 1062
Eco24I GRGCYC 3 cut(s) 429, 532, 657
Eco47I GGWCC 1 cut(s) 626
Eco53kI GAGCTC 1 cut(s) 655
Eco57I CTGAAG 2 cut(s) 155, 642
Eco72I CACGTG 1 cut(s) 435
EcoICRI GAGCTC 1 cut(s) 655
EcoO109I RGGNCCY 2 cut(s) 528, 626
EcoT14I CCWWGG 1 cut(s) 1062
EcoT38I GRGCYC 3 cut(s) 429, 532, 657
ErhI CCWWGG 1 cut(s) 1062
FaeI CATG 2 cut(s) 887, 934
FaiI YATR 7 cut(s) 63, 320, 861, 885, 932, 962, 1041
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FaqI GGGAC 2 cut(s) 92, 1028
FatI CATG 2 cut(s) 883, 930
FauI CCCGC 2 cut(s) 199, 1003
FbaI TGATCA 1 cut(s) 703
Fnu4HI GCNGC 8 cut(s) 195, 198, 238, 241, 366, 493, 969, 1032
FokI GGATG 4 cut(s) 215, 434, 989, 1079
FriOI GRGCYC 3 cut(s) 429, 532, 657
Fsp4HI GCNGC 8 cut(s) 195, 198, 238, 241, 366, 493, 969, 1032
GlaI GCGC 1 cut(s) 262
GluI GCNGC 8 cut(s) 195, 198, 238, 241, 366, 493, 969, 1032
HaeIII GGCC 4 cut(s) 268, 344, 530, 730
HapII CCGG 5 cut(s) 43, 355, 362, 551, 739
HgaI GACGC 2 cut(s) 46, 1028
HhaI GCGC 1 cut(s) 263
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 2 cut(s) 887, 934
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HindIII AAGCTT 3 cut(s) 97, 837, 915
HinfI GANTC 2 cut(s) 482, 1048
HpaII CCGG 5 cut(s) 43, 355, 362, 551, 739
HphI GGTGA 5 cut(s) 155, 491, 528, 698, 1050
Hpy166II GTNNAC 4 cut(s) 115, 536, 718, 952
Hpy188I TCNGA 7 cut(s) 303, 421, 606, 616, 692, 768, 913
Hpy188III TCNNGA 1 cut(s) 799
Hpy8I GTNNAC 4 cut(s) 115, 536, 718, 952
Hpy99I CGWCG 7 cut(s) 107, 122, 125, 134, 380, 1007, 1022
HpyAV CCTTC 4 cut(s) 133, 617, 743, 866
HpyCH4III ACNGT 1 cut(s) 956
HpyCH4IV ACGT 7 cut(s) 105, 144, 434, 447, 465, 570, 946
HpyCH4V TGCA 3 cut(s) 200, 249, 980
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 246, 977, 1028
HpyF3I CTNAG 4 cut(s) 302, 540, 605, 813
HpySE526I ACGT 7 cut(s) 105, 144, 434, 447, 465, 570, 946
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 2 cut(s) 887, 934
HspAI GCGC 1 cut(s) 261
Ksp22I TGATCA 1 cut(s) 703
KspI CCGCGG 1 cut(s) 195
Kzo9I GATC 6 cut(s) 703, 763, 768, 793, 901, 1055
LmnI GCTCC 9 cut(s) 77, 131, 180, 363, 405, 553, 586, 652, 741
Lsp1109I GCAGC 3 cut(s) 184, 352, 1018
LweI GCATC 2 cut(s) 258, 967
MaeII ACGT 7 cut(s) 105, 144, 434, 447, 465, 570, 946
MaeIII GTNAC 3 cut(s) 65, 479, 566
MalI GATC 6 cut(s) 705, 765, 770, 795, 903, 1057
MboI GATC 6 cut(s) 703, 763, 768, 793, 901, 1055
MboII GAAGA 4 cut(s) 44, 47, 460, 883
MfeI CAATTG 1 cut(s) 646
MhlI GDGCHC 3 cut(s) 429, 532, 657
MluCI AATT 7 cut(s) 508, 646, 866, 907, 936, 1042, 1079
MlyI GAGTC 1 cut(s) 476
MmeI TCCRAC 1 cut(s) 399
MnlI CCTC 4 cut(s) 191, 317, 666, 808
MroXI GAANNNNTTC 2 cut(s) 639, 722
MspA1I CMGCKG 1 cut(s) 194
MspI CCGG 5 cut(s) 43, 355, 362, 551, 739
MspR9I CCNGG 2 cut(s) 44, 362
MunI CAATTG 1 cut(s) 646
MvnI CGCG 4 cut(s) 57, 134, 194, 261
MwoI GCNNNNNNNGC 4 cut(s) 54, 246, 977, 1028
NciI CCSGG 2 cut(s) 44, 362
NdeII GATC 6 cut(s) 703, 763, 768, 793, 901, 1055
NlaIII CATG 2 cut(s) 887, 934
NmeAIII GCCGAG 1 cut(s) 464
NmuCI GTSAC 2 cut(s) 65, 479
PcsI WCGNNNNNNNCGW 3 cut(s) 75, 126, 150
PdmI GAANNNNTTC 2 cut(s) 639, 722
PfeI GAWTC 1 cut(s) 1048
PkrI GCNGC 8 cut(s) 196, 199, 239, 242, 367, 494, 970, 1033
PleI GAGTC 1 cut(s) 476
PmaCI CACGTG 1 cut(s) 435
PmlI CACGTG 1 cut(s) 435
PpsI GAGTC 1 cut(s) 476
Ppu21I YACGTR 1 cut(s) 435
PpuMI RGGWCCY 1 cut(s) 626
Psp124BI GAGCTC 1 cut(s) 657
Psp5II RGGWCCY 1 cut(s) 626
PspCI CACGTG 1 cut(s) 435
PspOMI GGGCCC 1 cut(s) 528
PspPI GGNCC 6 cut(s) 267, 343, 528, 529, 626, 728
PspPPI RGGWCCY 1 cut(s) 626
PstNI CAGNNNCTG 1 cut(s) 620
RsaI GTAC 1 cut(s) 756
RsaNI GTAC 1 cut(s) 755
SacI GAGCTC 1 cut(s) 657
SacII CCGCGG 1 cut(s) 195
SatI GCNGC 8 cut(s) 195, 198, 238, 241, 366, 493, 969, 1032
Sau3AI GATC 6 cut(s) 703, 763, 768, 793, 901, 1055
Sau96I GGNCC 6 cut(s) 267, 343, 528, 529, 626, 728
SchI GAGTC 1 cut(s) 476
ScrFI CCNGG 2 cut(s) 44, 362
SduI GDGCHC 3 cut(s) 429, 532, 657
SfaNI GCATC 2 cut(s) 258, 967
SfcI CTRYAG 1 cut(s) 61
Sfr303I CCGCGG 1 cut(s) 195
SgrBI CCGCGG 1 cut(s) 195
SinI GGWCC 1 cut(s) 626
Sse9I AATT 7 cut(s) 508, 646, 866, 907, 936, 1042, 1079
SsiI CCGC 9 cut(s) 178, 192, 194, 237, 240, 492, 515, 968, 1010
SspI AATATT 1 cut(s) 219
SstI GAGCTC 1 cut(s) 657
StyD4I CCNGG 2 cut(s) 42, 360
StyI CCWWGG 1 cut(s) 1062
TaaI ACNGT 1 cut(s) 956
TaiI ACGT 7 cut(s) 108, 147, 437, 450, 468, 573, 949
TaqI TCGA 5 cut(s) 5, 102, 153, 680, 864
TasI AATT 7 cut(s) 508, 646, 866, 907, 936, 1042, 1079
TauI GCSGC 5 cut(s) 197, 240, 243, 495, 971
TfiI GAWTC 1 cut(s) 1048
TscAI CASTG 3 cut(s) 16, 391, 706
TseFI GTSAC 2 cut(s) 65, 479
TseI GCWGC 3 cut(s) 197, 365, 1031
Tsp45I GTSAC 2 cut(s) 65, 479
TspDTI ATGAA 1 cut(s) 460
TspGWI ACGGA 2 cut(s) 71, 118
TspRI CASTG 3 cut(s) 16, 391, 706
VpaK11BI GGWCC 1 cut(s) 626
XapI RAATTY 2 cut(s) 866, 1079
XcmI CCANNNNNNNNNTGG 2 cut(s) 1060, 1079
XmnI GAANNNNTTC 2 cut(s) 639, 722
ZraI GACGTC 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.