pycom12g04010

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001599
Physical Location & Seq
Reverse (-)
3931477 .. 3932190
714 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g04010.1

Sequence Viewer

Length: 714 bp
ATGGGTTCCCTCCCTCACATAGTAGAAGATTGCTTGGGCGTCCTCCAAGTCTTTAGCGACGGTTCGATCAACCGTTGCTCTTTAACGGACATAAACTTCAACATTCCTGTCATTGATGACGGCTCCGTGGGCTTCAAAGACATCACTTTCGACAAAAAACACAGACTTTCCCTTCGTTTATACAAACCCAAATCGACAAACCAAGATTCCAAGCTCCCCGTGGTGTTCTACTTCCACGGTGGCGGCTTCTGTGTTGGCTCACACGTGTGGCCAAACTGCCACAATTGCTGCATCCGCCTGTCGTCGGGGCTCCAGGCCCTTGTGGTATCTCCCGACTATAGGCTCGCTCCAGAGCACAAGCTTCCGGCCGCAATTGACGATGCAGTGAGTGCAGTGGAGTGGCTGCAAAGGGAGGCTTCGGGAAAGAGCACGAATAACTGTGATGCATGGATTCGTGGTACAGCTGACTTCGACCGGGTTTTTGTATTGGGTGACTCTTCAGGTGGGAATATGGCTCACCACCTGGCGGTTCGGATCGCGACGGGGTCGATCAAGCCGGCTCCGATTAGGGTACGCGGGTTTGTGCTGCTGGCGCCATTTTTTGGTGGGGCGGAAAGGACAAAGTCAGAGGAAGGTCCTTGTGAGGTATTGTTGAGTTTGGAAATACTTGACAGGTATTCCTTCAAAACTTTTCGAGTCTTGTCCTTTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

238

Amino Acids

26.02

Weight (kDa)

7.06

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 56 - 117 2.4e-07 Carboxylesterase family
BD-FAE PF20434 60 - 177 4.3e-12 BD-FAE
Abhydrolase_3 PF07859 75 - 212 2.8e-38 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 592
AccB7I CCANNNNNTGG 1 cut(s) 602
AccII CGCG 2 cut(s) 539, 576
AciI CCGC 6 cut(s) 243, 295, 369, 527, 576, 611
AclWI GGATC 1 cut(s) 542
AcoI YGGCCR 2 cut(s) 269, 366
AcuI CTGAAG 1 cut(s) 483
AcvI CACGTG 1 cut(s) 265
AcyI GRCGYC 2 cut(s) 39, 593
AfaI GTAC 2 cut(s) 460, 573
AfiI CCNNNNNNNGG 4 cut(s) 304, 339, 526, 602
AflIII ACRYGT 2 cut(s) 262, 264
AgsI TTSAA 3 cut(s) 100, 136, 685
AjnI CCWGG 2 cut(s) 312, 522
AleI CACNNNNGTG 1 cut(s) 265
AluBI AGCT 3 cut(s) 214, 361, 464
AluI AGCT 3 cut(s) 214, 361, 464
Alw21I GWGCWC 2 cut(s) 357, 431
AlwI GGATC 1 cut(s) 542
AoxI GGCC 3 cut(s) 269, 315, 366
ApeKI GCWGC 3 cut(s) 288, 403, 586
ArsI GACNNNNNNTTYG 6 cut(s) 131, 156, 163, 188, 464, 496
AspLEI GCGC 1 cut(s) 595
AspS9I GGNCC 2 cut(s) 316, 635
AsuC2I CCSGG 1 cut(s) 476
AsuHPI GGTGA 2 cut(s) 503, 509
AvaII GGWCC 1 cut(s) 635
BaeI ACNNNNGTAYC 2 cut(s) 563, 596
BalI TGGCCA 1 cut(s) 271
BanI GGYRCC 1 cut(s) 592
BanII GRGCYC 1 cut(s) 312
BbrPI CACGTG 1 cut(s) 265
Bbv12I GWGCWC 2 cut(s) 357, 431
BbvI GCAGC 3 cut(s) 275, 390, 573
BceAI ACGGC 1 cut(s) 136
BciT130I CCWGG 2 cut(s) 314, 524
BcnI CCSGG 1 cut(s) 476
BfaI CTAG 1 cut(s) 712
BfmI CTRYAG 1 cut(s) 337
BfoI RGCGCY 1 cut(s) 596
BisI GCNGC 5 cut(s) 244, 289, 369, 404, 587
BlsI GCNGC 5 cut(s) 245, 290, 370, 405, 588
Bme1390I CCNGG 3 cut(s) 314, 476, 524
Bme18I GGWCC 1 cut(s) 635
BmgT120I GGNCC 2 cut(s) 316, 635
BmiI GGNNCC 5 cut(s) 7, 124, 311, 561, 594
BmrFI CCNGG 3 cut(s) 314, 476, 524
BmsI GCATC 3 cut(s) 300, 370, 433
BpmI CTGGAG 2 cut(s) 296, 333
BpuMI CCSGG 1 cut(s) 476
BsaAI YACGTR 1 cut(s) 265
BsaHI GRCGYC 2 cut(s) 39, 593
BsaJI CCNNGG 3 cut(s) 126, 219, 235
Bsc4I CCNNNNNNNGG 4 cut(s) 304, 339, 526, 602
Bse118I RCCGGY 1 cut(s) 556
BseBI CCWGG 2 cut(s) 314, 524
BseDI CCNNGG 3 cut(s) 126, 219, 235
BseGI GGATG 1 cut(s) 291
BseLI CCNNNNNNNGG 4 cut(s) 304, 339, 526, 602
BseX3I CGGCCG 1 cut(s) 366
BseXI GCAGC 3 cut(s) 275, 390, 573
BsgI GTGCAG 1 cut(s) 411
Bsh1236I CGCG 2 cut(s) 539, 576
Bsh1285I CGRYCG 2 cut(s) 369, 475
BshFI GGCC 3 cut(s) 271, 317, 368
BshNI GGYRCC 1 cut(s) 592
BsiEI CGRYCG 2 cut(s) 369, 475
BsiHKAI GWGCWC 2 cut(s) 357, 431
BsiSI CCGG 3 cut(s) 365, 475, 557
BslI CCNNNNNNNGG 4 cut(s) 304, 339, 526, 602
BsnI GGCC 3 cut(s) 271, 317, 368
Bsp1286I GDGCHC 3 cut(s) 312, 357, 431
Bsp143I GATC 3 cut(s) 66, 534, 549
Bsp68I TCGCGA 1 cut(s) 539
BspACI CCGC 6 cut(s) 243, 295, 369, 527, 576, 611
BspANI GGCC 3 cut(s) 271, 317, 368
BspFNI CGCG 2 cut(s) 539, 576
BspLI GGNNCC 5 cut(s) 7, 124, 311, 561, 594
BspPI GGATC 1 cut(s) 542
BspT107I GGYRCC 1 cut(s) 592
BsrFI RCCGGY 1 cut(s) 556
BssAI RCCGGY 1 cut(s) 556
BssECI CCNNGG 3 cut(s) 126, 219, 235
BssMI GATC 3 cut(s) 66, 534, 549
BssNI GRCGYC 2 cut(s) 39, 593
Bst2UI CCWGG 2 cut(s) 314, 524
Bst4CI ACNGT 4 cut(s) 62, 74, 239, 440
Bst6I CTCTTC 1 cut(s) 502
BstACI GRCGYC 2 cut(s) 39, 593
BstAPI GCANNNNNTGC 1 cut(s) 389
BstBAI YACGTR 1 cut(s) 265
BstC8I GCNNGC 3 cut(s) 345, 558, 591
BstDSI CCRYGG 3 cut(s) 126, 219, 235
BstF5I GGATG 1 cut(s) 291
BstFNI CGCG 2 cut(s) 539, 576
BstH2I RGCGCY 1 cut(s) 596
BstHHI GCGC 1 cut(s) 595
BstKTI GATC 3 cut(s) 69, 537, 552
BstMBI GATC 3 cut(s) 66, 534, 549
BstMCI CGRYCG 2 cut(s) 369, 475
BstMWI GCNNNNNNNGC 5 cut(s) 129, 285, 294, 389, 592
BstNI CCWGG 2 cut(s) 314, 524
BstSCI CCNGG 3 cut(s) 312, 474, 522
BstSFI CTRYAG 1 cut(s) 337
BstUI CGCG 2 cut(s) 539, 576
BstV1I GCAGC 3 cut(s) 275, 390, 573
BstZI CGGCCG 1 cut(s) 366
BsuRI GGCC 3 cut(s) 271, 317, 368
BtgI CCRYGG 3 cut(s) 126, 219, 235
BtsCI GGATG 1 cut(s) 291
BtsI GCAGTG 2 cut(s) 390, 399
BtsIMutI CAGTG 2 cut(s) 390, 399
BtuMI TCGCGA 1 cut(s) 539
Cac8I GCNNGC 3 cut(s) 345, 558, 591
CfoI GCGC 1 cut(s) 595
Cfr10I RCCGGY 1 cut(s) 556
Cfr13I GGNCC 2 cut(s) 316, 635
CseI GACGC 1 cut(s) 28
Csp6I GTAC 2 cut(s) 459, 572
CviAII CATG 1 cut(s) 447
CviQI GTAC 2 cut(s) 459, 572
DinI GGCGCC 1 cut(s) 594
DpnI GATC 3 cut(s) 68, 536, 551
DpnII GATC 3 cut(s) 66, 534, 549
EaeI YGGCCR 2 cut(s) 269, 366
EagI CGGCCG 1 cut(s) 366
Eam1104I CTCTTC 1 cut(s) 502
EarI CTCTTC 1 cut(s) 502
EciI GGCGGA 2 cut(s) 284, 626
EclXI CGGCCG 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 312
Eco47I GGWCC 1 cut(s) 635
Eco52I CGGCCG 1 cut(s) 366
Eco57I CTGAAG 1 cut(s) 483
Eco72I CACGTG 1 cut(s) 265
EcoO109I RGGNCCY 2 cut(s) 316, 635
EcoRII CCWGG 2 cut(s) 312, 522
EcoT22I ATGCAT 1 cut(s) 448
EcoT38I GRGCYC 1 cut(s) 312
EgeI GGCGCC 1 cut(s) 594
EheI GGCGCC 1 cut(s) 594
FaeI CATG 1 cut(s) 450
FaiI YATR 6 cut(s) 20, 92, 181, 339, 448, 512
FalI AAGNNNNNCTT 2 cut(s) 400, 432
FatI CATG 1 cut(s) 446
FauI CCCGC 1 cut(s) 569
Fnu4HI GCNGC 5 cut(s) 244, 289, 369, 404, 587
FokI GGATG 1 cut(s) 278
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 5 cut(s) 244, 289, 369, 404, 587
FspBI CTAG 1 cut(s) 712
GlaI GCGC 1 cut(s) 594
GluI GCNGC 5 cut(s) 244, 289, 369, 404, 587
GsuI CTGGAG 2 cut(s) 296, 333
HaeII RGCGCY 1 cut(s) 596
HaeIII GGCC 3 cut(s) 271, 317, 368
HapII CCGG 3 cut(s) 365, 475, 557
HgaI GACGC 1 cut(s) 28
HhaI GCGC 1 cut(s) 595
Hin1I GRCGYC 2 cut(s) 39, 593
Hin1II CATG 1 cut(s) 450
Hin6I GCGC 1 cut(s) 593
HinP1I GCGC 1 cut(s) 593
HindIII AAGCTT 1 cut(s) 359
HinfI GANTC 4 cut(s) 206, 451, 494, 696
HpaII CCGG 3 cut(s) 365, 475, 557
HphI GGTGA 2 cut(s) 503, 509
Hpy188I TCNGA 3 cut(s) 534, 564, 628
Hpy188III TCNNGA 4 cut(s) 332, 350, 420, 538
Hpy99I CGWCG 3 cut(s) 62, 307, 544
HpyAV CCTTC 3 cut(s) 182, 626, 691
HpyCH4III ACNGT 4 cut(s) 62, 74, 239, 440
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 5 cut(s) 291, 383, 392, 406, 446
HpyF10VI GCNNNNNNNGC 5 cut(s) 129, 285, 294, 389, 592
HpySE526I ACGT 1 cut(s) 264
Hsp92I GRCGYC 2 cut(s) 39, 593
Hsp92II CATG 1 cut(s) 450
HspAI GCGC 1 cut(s) 593
KasI GGCGCC 1 cut(s) 592
KroI GCCGGC 1 cut(s) 556
KroNI GCCGGC 1 cut(s) 558
Kzo9I GATC 3 cut(s) 66, 534, 549
LmnI GCTCC 5 cut(s) 128, 219, 315, 352, 565
Lsp1109I GCAGC 3 cut(s) 275, 390, 573
LweI GCATC 3 cut(s) 300, 370, 433
MaeI CTAG 1 cut(s) 712
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 1 cut(s) 491
MalI GATC 3 cut(s) 68, 536, 551
MboI GATC 3 cut(s) 66, 534, 549
MboII GAAGA 2 cut(s) 38, 489
MfeI CAATTG 2 cut(s) 283, 372
MhlI GDGCHC 3 cut(s) 312, 357, 431
MlsI TGGCCA 1 cut(s) 271
MluCI AATT 2 cut(s) 283, 372
MluNI TGGCCA 1 cut(s) 271
Mly113I GGCGCC 1 cut(s) 593
MlyI GAGTC 2 cut(s) 488, 705
MnlI CCTC 6 cut(s) 20, 24, 53, 406, 622, 637
Mox20I TGGCCA 1 cut(s) 271
Mph1103I ATGCAT 1 cut(s) 448
MroNI GCCGGC 1 cut(s) 556
MscI TGGCCA 1 cut(s) 271
MseI TTAA 1 cut(s) 83
MslI CAYNNNNRTG 1 cut(s) 265
Msp20I TGGCCA 1 cut(s) 271
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 3 cut(s) 365, 475, 557
MspR9I CCNGG 3 cut(s) 314, 476, 524
MunI CAATTG 2 cut(s) 283, 372
MvaI CCWGG 2 cut(s) 314, 524
MvnI CGCG 2 cut(s) 539, 576
MwoI GCNNNNNNNGC 5 cut(s) 129, 285, 294, 389, 592
NaeI GCCGGC 1 cut(s) 558
NarI GGCGCC 1 cut(s) 593
NciI CCSGG 1 cut(s) 476
NdeII GATC 3 cut(s) 66, 534, 549
NgoMIV GCCGGC 1 cut(s) 556
NlaIII CATG 1 cut(s) 450
NlaIV GGNNCC 5 cut(s) 7, 124, 311, 561, 594
NmuCI GTSAC 1 cut(s) 491
NruI TCGCGA 1 cut(s) 539
NsiI ATGCAT 1 cut(s) 448
OliI CACNNNNGTG 1 cut(s) 265
PdiI GCCGGC 1 cut(s) 558
PfeI GAWTC 2 cut(s) 206, 451
PflFI GACNNNGTC 2 cut(s) 544, 622
PflMI CCANNNNNTGG 1 cut(s) 602
PkrI GCNGC 5 cut(s) 245, 290, 370, 405, 588
PleI GAGTC 2 cut(s) 488, 704
PluTI GGCGCC 1 cut(s) 596
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
PpsI GAGTC 2 cut(s) 488, 704
Ppu21I YACGTR 1 cut(s) 265
PpuMI RGGWCCY 1 cut(s) 635
Psp5II RGGWCCY 1 cut(s) 635
Psp6I CCWGG 2 cut(s) 312, 522
PspCI CACGTG 1 cut(s) 265
PspGI CCWGG 2 cut(s) 312, 522
PspN4I GGNNCC 5 cut(s) 7, 124, 311, 561, 594
PspPI GGNCC 2 cut(s) 316, 635
PspPPI RGGWCCY 1 cut(s) 635
PsyI GACNNNGTC 2 cut(s) 544, 622
PvuII CAGCTG 1 cut(s) 464
RruI TCGCGA 1 cut(s) 539
RsaI GTAC 2 cut(s) 460, 573
RsaNI GTAC 2 cut(s) 459, 572
RseI CAYNNNNRTG 1 cut(s) 265
SaqAI TTAA 1 cut(s) 83
SatI GCNGC 5 cut(s) 244, 289, 369, 404, 587
Sau3AI GATC 3 cut(s) 66, 534, 549
Sau96I GGNCC 2 cut(s) 316, 635
SchI GAGTC 2 cut(s) 488, 705
ScrFI CCNGG 3 cut(s) 314, 476, 524
SduI GDGCHC 3 cut(s) 312, 357, 431
SetI ASST 9 cut(s) 216, 267, 363, 466, 505, 525, 637, 648, 677
SfaNI GCATC 3 cut(s) 300, 370, 433
SfcI CTRYAG 1 cut(s) 337
SfoI GGCGCC 1 cut(s) 594
SinI GGWCC 1 cut(s) 635
SmiMI CAYNNNNRTG 1 cut(s) 265
Sse9I AATT 2 cut(s) 283, 372
SsiI CCGC 6 cut(s) 243, 295, 369, 527, 576, 611
SspDI GGCGCC 1 cut(s) 592
SspMI CTAG 1 cut(s) 712
StyD4I CCNGG 3 cut(s) 312, 474, 522
TaaI ACNGT 4 cut(s) 62, 74, 239, 440
TaiI ACGT 1 cut(s) 267
TaqI TCGA 6 cut(s) 65, 150, 194, 471, 548, 694
TasI AATT 2 cut(s) 283, 372
TauI GCSGC 2 cut(s) 246, 371
TfiI GAWTC 2 cut(s) 206, 451
Tru1I TTAA 1 cut(s) 83
Tru9I TTAA 1 cut(s) 83
TscAI CASTG 2 cut(s) 390, 399
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 3 cut(s) 288, 403, 586
Tsp45I GTSAC 1 cut(s) 491
TspGWI ACGGA 2 cut(s) 101, 115
TspRI CASTG 2 cut(s) 390, 399
Tth111I GACNNNGTC 2 cut(s) 544, 622
Van91I CCANNNNNTGG 1 cut(s) 602
VpaK11BI GGWCC 1 cut(s) 635
XcmI CCANNNNNNNNNTGG 1 cut(s) 217
XspI CTAG 1 cut(s) 712
Zsp2I ATGCAT 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.