Rroxscaffold_6G00403880

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001599
Physical Location & Seq
Forward (+)
26340136 .. 26345058
4923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00403880.1

Sequence Viewer

Length: 996 bp
ATGAACTTCGATCTCATATCTAATGCCTACAGTGATCTGGAGACAATCCAGAACCAGAAGCGAACTCGAATCGGAATCCATACTCATGCCCAGATCCTAGATTCGACCGTTAACGACAACTCCGTGGACTTCAAAGACGTCGTTTTCGACAAAAAGCTCAACATTTCCCTACGTGTGTACAGACCTAAAAGCCATGCGTCTCCGACGCCCGCAAAGCTCCCCGTGGTGTTTTACTTCCACGGGGGCGGTTTCTGCGTCGGCTCGCGGGTGTGGCCCAACTGCCACAACTGCTGCATCCGCCTGGCGTCTGAGCTCAACTCGCTCGTCGTGGCGCCAGACTACCGGCTCGCCCCCGAGCACAAGCTCCCGGCCGCAGTGGATGACGCGGTGAGTGCGGTGGAGTGGCTGCAGAGGGAGGCTTTGATGAGTGAGGGCAGTGGTGATTGTGATGCATGGCTGGGTGGTGGTATGGTTGACTTTGACCGGGTTTATGTGGTGGGCGACTCATCAGGAGGTAATATGGCTCACCACGTGGCCGTACGGCTTGGAGCGGGGTCGGTCAAGGCGGCGCCGGTGCGGGTACGCGGATATGTGCTTTTGGCGCCATTTTTCGGTGGAGTGGAGAGGACAAAGTCGGAGGAGGGTCCGTCGGAGACGTTTTTGAATTTGGAGATACTTGACAGTTCATATTTTGGAGGCTCTCATTGCCAAAAGGAGAAACCAGGGATCATCCAATGGCCAACCCATTTGGACCAAACAGCCCCCAGCCTTGAGGAGGTGGATCTTGATCCCATCTTGGTTATTGTGGGTGGCAATGAAATGTTGAAAGATAGAGTGGAGATCTACTCGAGGAGACTGAAAGAGTTGGGAAAGAAGATTGAGTATGTGGATTTTCATGGACAACAACATGGCTTTCTCACAAATGATCCATACTCAGAAGTGGCAAATGAAGCACTGCAAGTTATTAAGAGATTTATGATTGAAAACTCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

331

Amino Acids

36.67

Weight (kDa)

5.64

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 57 - 118 1.9e-06 Carboxylesterase family
BD-FAE PF20434 59 - 180 6.4e-10 BD-FAE
Abhydrolase_3 PF07859 76 - 306 1.8e-48 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 141
AccB1I GGYRCC 3 cut(s) 331, 568, 601
AccBSI CCGCTC 1 cut(s) 551
AccII CGCG 3 cut(s) 265, 386, 585
AclWI GGATC 5 cut(s) 88, 734, 782, 789, 920
AcoI YGGCCR 3 cut(s) 369, 534, 737
AcsI RAATTY 1 cut(s) 664
AcvI CACGTG 1 cut(s) 532
AcyI GRCGYC 6 cut(s) 138, 206, 305, 332, 569, 602
AdeI CACNNNGTG 1 cut(s) 532
AfaI GTAC 3 cut(s) 179, 540, 582
AfiI CCNNNNNNNGG 2 cut(s) 611, 775
AflIII ACRYGT 1 cut(s) 172
AgsI TTSAA 4 cut(s) 133, 664, 826, 983
AjnI CCWGG 2 cut(s) 300, 721
AluBI AGCT 4 cut(s) 157, 217, 313, 364
AluI AGCT 4 cut(s) 157, 217, 313, 364
Alw21I GWGCWC 2 cut(s) 315, 360
Alw26I GTCTC 4 cut(s) 35, 204, 647, 847
AlwI GGATC 5 cut(s) 88, 734, 782, 789, 920
Ama87I CYCGRG 2 cut(s) 353, 847
AoxI GGCC 4 cut(s) 272, 369, 534, 737
ApeKI GCWGC 2 cut(s) 291, 406
ApoI RAATTY 1 cut(s) 664
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AspLEI GCGC 3 cut(s) 334, 571, 604
AspS9I GGNCC 3 cut(s) 273, 644, 751
AsuC2I CCSGG 2 cut(s) 368, 485
AsuHPI GGTGA 3 cut(s) 400, 452, 518
AvaI CYCGRG 2 cut(s) 353, 847
AvaII GGWCC 2 cut(s) 644, 751
BalI TGGCCA 1 cut(s) 739
BanI GGYRCC 3 cut(s) 331, 568, 601
BanII GRGCYC 1 cut(s) 315
BbrPI CACGTG 1 cut(s) 532
Bbv12I GWGCWC 2 cut(s) 315, 360
BbvI GCAGC 2 cut(s) 278, 393
BccI CCATC 1 cut(s) 800
BceAI ACGGC 2 cut(s) 521, 557
BciT130I CCWGG 2 cut(s) 302, 723
BcnI CCSGG 2 cut(s) 368, 485
BcoDI GTCTC 4 cut(s) 35, 204, 647, 847
BfaI CTAG 1 cut(s) 98
BfmI CTRYAG 2 cut(s) 28, 407
BfoI RGCGCY 3 cut(s) 335, 572, 605
BglII AGATCT 1 cut(s) 840
BisI GCNGC 4 cut(s) 292, 372, 407, 567
BlsI GCNGC 4 cut(s) 293, 373, 408, 568
Bme1390I CCNGG 4 cut(s) 302, 368, 485, 723
Bme18I GGWCC 2 cut(s) 644, 751
BmeT110I CYCGRG 2 cut(s) 353, 847
BmgT120I GGNCC 3 cut(s) 273, 644, 751
BmiI GGNNCC 4 cut(s) 333, 570, 603, 645
BmrFI CCNGG 4 cut(s) 302, 368, 485, 723
BmsI GCATC 2 cut(s) 303, 439
BplI GAGNNNNNCTC 4 cut(s) 302, 334, 830, 862
BpmI CTGGAG 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 791
BpuMI CCSGG 2 cut(s) 368, 485
BsaAI YACGTR 2 cut(s) 173, 532
BsaBI GATNNNNATC 1 cut(s) 786
BsaHI GRCGYC 6 cut(s) 138, 206, 305, 332, 569, 602
BsaJI CCNNGG 4 cut(s) 123, 222, 238, 722
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 632, 662
Bsc4I CCNNNNNNNGG 2 cut(s) 611, 775
Bse118I RCCGGY 2 cut(s) 342, 571
Bse3DI GCAATG 2 cut(s) 703, 820
Bse8I GATNNNNATC 1 cut(s) 786
BseBI CCWGG 2 cut(s) 302, 723
BseDI CCNNGG 4 cut(s) 123, 222, 238, 722
BseGI GGATG 3 cut(s) 294, 385, 729
BseJI GATNNNNATC 1 cut(s) 786
BseLI CCNNNNNNNGG 2 cut(s) 611, 775
BseMI GCAATG 2 cut(s) 703, 820
BseMII CTCAG 2 cut(s) 300, 948
BseRI GAGGAG 3 cut(s) 653, 788, 865
BseX3I CGGCCG 1 cut(s) 369
BseXI GCAGC 2 cut(s) 278, 393
BseYI CCCAGC 2 cut(s) 457, 764
Bsh1236I CGCG 3 cut(s) 265, 386, 585
Bsh1285I CGRYCG 2 cut(s) 108, 372
BshFI GGCC 4 cut(s) 274, 371, 536, 739
BshNI GGYRCC 3 cut(s) 331, 568, 601
BsiEI CGRYCG 2 cut(s) 108, 372
BsiHKAI GWGCWC 2 cut(s) 315, 360
BsiHKCI CYCGRG 2 cut(s) 353, 847
BsiSI CCGG 4 cut(s) 343, 368, 484, 572
BsiWI CGTACG 1 cut(s) 538
BslI CCNNNNNNNGG 2 cut(s) 611, 775
BsmAI GTCTC 4 cut(s) 35, 204, 647, 847
BsmBI CGTCTC 2 cut(s) 204, 647
BsnI GGCC 4 cut(s) 274, 371, 536, 739
BsoBI CYCGRG 2 cut(s) 353, 847
Bsp1286I GDGCHC 2 cut(s) 315, 360
Bsp1407I TGTACA 1 cut(s) 177
Bsp143I GATC 8 cut(s) 10, 34, 93, 726, 781, 787, 840, 925
BspANI GGCC 4 cut(s) 274, 371, 536, 739
BspCNI CTCAG 2 cut(s) 301, 947
BspFNI CGCG 3 cut(s) 265, 386, 585
BspLI GGNNCC 4 cut(s) 333, 570, 603, 645
BspMAI CTGCAG 1 cut(s) 411
BspPI GGATC 5 cut(s) 88, 734, 782, 789, 920
BspT107I GGYRCC 3 cut(s) 331, 568, 601
BsrBI CCGCTC 1 cut(s) 551
BsrDI GCAATG 2 cut(s) 703, 820
BsrFI RCCGGY 2 cut(s) 342, 571
BsrGI TGTACA 1 cut(s) 177
BssAI RCCGGY 2 cut(s) 342, 571
BssECI CCNNGG 4 cut(s) 123, 222, 238, 722
BssMI GATC 8 cut(s) 10, 34, 93, 726, 781, 787, 840, 925
BssNI GRCGYC 6 cut(s) 138, 206, 305, 332, 569, 602
Bst2UI CCWGG 2 cut(s) 302, 723
Bst4CI ACNGT 3 cut(s) 32, 109, 683
BstACI GRCGYC 6 cut(s) 138, 206, 305, 332, 569, 602
BstAUI TGTACA 1 cut(s) 177
BstBAI YACGTR 2 cut(s) 173, 532
BstC8I GCNNGC 3 cut(s) 210, 263, 348
BstDEI CTNAG 2 cut(s) 309, 934
BstDSI CCRYGG 3 cut(s) 123, 222, 238
BstENI CCTNNNNNAGG 1 cut(s) 773
BstF5I GGATG 3 cut(s) 294, 385, 729
BstFNI CGCG 3 cut(s) 265, 386, 585
BstH2I RGCGCY 3 cut(s) 335, 572, 605
BstHHI GCGC 3 cut(s) 334, 571, 604
BstKTI GATC 8 cut(s) 13, 37, 96, 729, 784, 790, 843, 928
BstMAI GTCTC 4 cut(s) 35, 204, 647, 847
BstMBI GATC 8 cut(s) 10, 34, 93, 726, 781, 787, 840, 925
BstMCI CGRYCG 2 cut(s) 108, 372
BstNI CCWGG 2 cut(s) 302, 723
BstSCI CCNGG 4 cut(s) 300, 366, 483, 721
BstSFI CTRYAG 2 cut(s) 28, 407
BstUI CGCG 3 cut(s) 265, 386, 585
BstV1I GCAGC 2 cut(s) 278, 393
BstX2I RGATCY 3 cut(s) 93, 781, 840
BstYI RGATCY 3 cut(s) 93, 781, 840
BstZI CGGCCG 1 cut(s) 369
BsuRI GGCC 4 cut(s) 274, 371, 536, 739
BtgI CCRYGG 3 cut(s) 123, 222, 238
BtsCI GGATG 3 cut(s) 294, 385, 729
BtsI GCAGTG 3 cut(s) 381, 442, 953
BtsIMutI CAGTG 4 cut(s) 37, 381, 442, 953
Cac8I GCNNGC 3 cut(s) 210, 263, 348
CfoI GCGC 3 cut(s) 334, 571, 604
Cfr10I RCCGGY 2 cut(s) 342, 571
Cfr13I GGNCC 3 cut(s) 273, 644, 751
CseI GACGC 5 cut(s) 186, 214, 244, 294, 392
Csp6I GTAC 3 cut(s) 178, 539, 581
CviAII CATG 5 cut(s) 86, 194, 453, 896, 908
CviQI GTAC 3 cut(s) 178, 539, 581
DdeI CTNAG 2 cut(s) 309, 934
DinI GGCGCC 3 cut(s) 333, 570, 603
DpnI GATC 8 cut(s) 12, 36, 95, 728, 783, 789, 842, 927
DpnII GATC 8 cut(s) 10, 34, 93, 726, 781, 787, 840, 925
DraIII CACNNNGTG 1 cut(s) 532
EaeI YGGCCR 3 cut(s) 369, 534, 737
EagI CGGCCG 1 cut(s) 369
EciI GGCGGA 1 cut(s) 287
Ecl136II GAGCTC 1 cut(s) 313
EclXI CGGCCG 1 cut(s) 369
Eco24I GRGCYC 1 cut(s) 315
Eco47I GGWCC 2 cut(s) 644, 751
Eco52I CGGCCG 1 cut(s) 369
Eco53kI GAGCTC 1 cut(s) 313
Eco72I CACGTG 1 cut(s) 532
Eco88I CYCGRG 2 cut(s) 353, 847
EcoICRI GAGCTC 1 cut(s) 313
EcoNI CCTNNNNNAGG 1 cut(s) 773
EcoRII CCWGG 2 cut(s) 300, 721
EcoT22I ATGCAT 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 315
EgeI GGCGCC 3 cut(s) 333, 570, 603
EheI GGCGCC 3 cut(s) 333, 570, 603
Esp3I CGTCTC 2 cut(s) 204, 647
FaeI CATG 5 cut(s) 89, 197, 456, 899, 911
FatI CATG 5 cut(s) 85, 193, 452, 895, 907
FauI CCCGC 4 cut(s) 217, 258, 544, 570
Fnu4HI GCNGC 4 cut(s) 292, 372, 407, 567
FokI GGATG 3 cut(s) 281, 392, 716
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 4 cut(s) 292, 372, 407, 567
FspBI CTAG 1 cut(s) 98
GlaI GCGC 3 cut(s) 333, 570, 603
GluI GCNGC 4 cut(s) 292, 372, 407, 567
GsaI CCCAGC 2 cut(s) 461, 768
GsuI CTGGAG 1 cut(s) 59
HaeII RGCGCY 3 cut(s) 335, 572, 605
HaeIII GGCC 4 cut(s) 274, 371, 536, 739
HapII CCGG 4 cut(s) 343, 368, 484, 572
HgaI GACGC 5 cut(s) 186, 214, 244, 294, 392
HhaI GCGC 3 cut(s) 334, 571, 604
Hin1I GRCGYC 6 cut(s) 138, 206, 305, 332, 569, 602
Hin1II CATG 5 cut(s) 89, 197, 456, 899, 911
Hin6I GCGC 3 cut(s) 332, 569, 602
HinP1I GCGC 3 cut(s) 332, 569, 602
HincII GTYRAC 2 cut(s) 112, 475
HindII GTYRAC 2 cut(s) 112, 475
HinfI GANTC 4 cut(s) 69, 75, 101, 503
HpaI GTTAAC 1 cut(s) 112
HpaII CCGG 4 cut(s) 343, 368, 484, 572
HphI GGTGA 3 cut(s) 400, 452, 518
Hpy166II GTNNAC 4 cut(s) 112, 127, 178, 475
Hpy188I TCNGA 6 cut(s) 74, 204, 310, 637, 652, 937
Hpy188III TCNNGA 4 cut(s) 38, 49, 510, 785
Hpy8I GTNNAC 4 cut(s) 112, 127, 178, 475
Hpy99I CGWCG 5 cut(s) 143, 208, 260, 329, 652
HpyCH4III ACNGT 3 cut(s) 32, 109, 683
HpyCH4IV ACGT 4 cut(s) 138, 172, 531, 656
HpyCH4V TGCA 4 cut(s) 294, 409, 452, 958
HpyF3I CTNAG 2 cut(s) 309, 934
HpySE526I ACGT 4 cut(s) 138, 172, 531, 656
Hsp92I GRCGYC 6 cut(s) 138, 206, 305, 332, 569, 602
Hsp92II CATG 5 cut(s) 89, 197, 456, 899, 911
HspAI GCGC 3 cut(s) 332, 569, 602
KasI GGCGCC 3 cut(s) 331, 568, 601
KspAI GTTAAC 1 cut(s) 112
Kzo9I GATC 8 cut(s) 10, 34, 93, 726, 781, 787, 840, 925
LmnI GCTCC 3 cut(s) 222, 369, 548
Lsp1109I GCAGC 2 cut(s) 278, 393
LweI GCATC 2 cut(s) 303, 439
MaeI CTAG 1 cut(s) 98
MaeII ACGT 4 cut(s) 138, 172, 531, 656
MalI GATC 8 cut(s) 12, 36, 95, 728, 783, 789, 842, 927
MbiI CCGCTC 1 cut(s) 551
MboI GATC 8 cut(s) 10, 34, 93, 726, 781, 787, 840, 925
MboII GAAGA 1 cut(s) 886
MflI RGATCY 3 cut(s) 93, 781, 840
MhlI GDGCHC 2 cut(s) 315, 360
MlsI TGGCCA 1 cut(s) 739
MluCI AATT 1 cut(s) 664
MluNI TGGCCA 1 cut(s) 739
Mly113I GGCGCC 3 cut(s) 332, 569, 602
MlyI GAGTC 1 cut(s) 497
MmeI TCCRAC 3 cut(s) 227, 615, 630
Mox20I TGGCCA 1 cut(s) 739
Mph1103I ATGCAT 1 cut(s) 454
MscI TGGCCA 1 cut(s) 739
MseI TTAA 2 cut(s) 111, 966
MslI CAYNNNNRTG 1 cut(s) 84
Msp20I TGGCCA 1 cut(s) 739
MspI CCGG 4 cut(s) 343, 368, 484, 572
MspR9I CCNGG 4 cut(s) 302, 368, 485, 723
MvaI CCWGG 2 cut(s) 302, 723
MvnI CGCG 3 cut(s) 265, 386, 585
NarI GGCGCC 3 cut(s) 332, 569, 602
NciI CCSGG 2 cut(s) 368, 485
NdeII GATC 8 cut(s) 10, 34, 93, 726, 781, 787, 840, 925
NlaIII CATG 5 cut(s) 89, 197, 456, 899, 911
NlaIV GGNNCC 4 cut(s) 333, 570, 603, 645
NsiI ATGCAT 1 cut(s) 454
PaeR7I CTCGAG 1 cut(s) 847
PcsI WCGNNNNNNNCGW 2 cut(s) 120, 144
PfeI GAWTC 3 cut(s) 69, 75, 101
Pfl23II CGTACG 1 cut(s) 538
PflFI GACNNNGTC 1 cut(s) 631
PkrI GCNGC 4 cut(s) 293, 373, 408, 568
PleI GAGTC 1 cut(s) 497
PluTI GGCGCC 3 cut(s) 335, 572, 605
PmaCI CACGTG 1 cut(s) 532
PmlI CACGTG 1 cut(s) 532
PpsI GAGTC 1 cut(s) 497
Ppu21I YACGTR 2 cut(s) 173, 532
Psp124BI GAGCTC 1 cut(s) 315
Psp6I CCWGG 2 cut(s) 300, 721
PspCI CACGTG 1 cut(s) 532
PspFI CCCAGC 2 cut(s) 457, 764
PspGI CCWGG 2 cut(s) 300, 721
PspLI CGTACG 1 cut(s) 538
PspN4I GGNNCC 4 cut(s) 333, 570, 603, 645
PspPI GGNCC 3 cut(s) 273, 644, 751
PspXI VCTCGAGB 1 cut(s) 847
PstI CTGCAG 1 cut(s) 411
PsuI RGATCY 3 cut(s) 93, 781, 840
PsyI GACNNNGTC 1 cut(s) 631
RsaI GTAC 3 cut(s) 179, 540, 582
RsaNI GTAC 3 cut(s) 178, 539, 581
RseI CAYNNNNRTG 1 cut(s) 84
SacI GAGCTC 1 cut(s) 315
SaqAI TTAA 2 cut(s) 111, 966
SatI GCNGC 4 cut(s) 292, 372, 407, 567
Sau3AI GATC 8 cut(s) 10, 34, 93, 726, 781, 787, 840, 925
Sau96I GGNCC 3 cut(s) 273, 644, 751
SchI GAGTC 1 cut(s) 497
ScrFI CCNGG 4 cut(s) 302, 368, 485, 723
SduI GDGCHC 2 cut(s) 315, 360
SfaNI GCATC 2 cut(s) 303, 439
SfcI CTRYAG 2 cut(s) 28, 407
SfoI GGCGCC 3 cut(s) 333, 570, 603
Sfr274I CTCGAG 1 cut(s) 847
SgrAI CRCCGGYG 1 cut(s) 571
SinI GGWCC 2 cut(s) 644, 751
SlaI CTCGAG 1 cut(s) 847
SmiMI CAYNNNNRTG 1 cut(s) 84
SmlI CTYRAG 2 cut(s) 770, 847
SmoI CTYRAG 2 cut(s) 770, 847
Sse9I AATT 1 cut(s) 664
SspDI GGCGCC 3 cut(s) 331, 568, 601
SspMI CTAG 1 cut(s) 98
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 4 cut(s) 300, 366, 483, 721
TaaI ACNGT 3 cut(s) 32, 109, 683
TaiI ACGT 4 cut(s) 141, 175, 534, 659
TaqI TCGA 5 cut(s) 9, 67, 104, 147, 848
TaqII GACCGA 1 cut(s) 547
TasI AATT 1 cut(s) 664
TatI WGTACW 1 cut(s) 177
TauI GCSGC 2 cut(s) 374, 569
TfiI GAWTC 3 cut(s) 69, 75, 101
Tru1I TTAA 2 cut(s) 111, 966
Tru9I TTAA 2 cut(s) 111, 966
TscAI CASTG 4 cut(s) 37, 381, 442, 960
TseI GCWGC 2 cut(s) 291, 406
TspDTI ATGAA 5 cut(s) 17, 675, 831, 884, 963
TspGWI ACGGA 2 cut(s) 112, 636
TspRI CASTG 4 cut(s) 37, 381, 442, 960
Tth111I GACNNNGTC 1 cut(s) 631
VpaK11BI GGWCC 2 cut(s) 644, 751
XagI CCTNNNNNAGG 1 cut(s) 773
XapI RAATTY 1 cut(s) 664
XhoI CTCGAG 1 cut(s) 847
XspI CTAG 1 cut(s) 98
ZraI GACGTC 1 cut(s) 139
Zsp2I ATGCAT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.