RLG00000023715

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001599
Physical Location & Seq
Forward (+)
27395475 .. 27397382
1908 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023715

Sequence Viewer

Length: 999 bp
ATGGGTTCCCGACCTCACATTGTAGATGACCTCGGTGGCAATCTCCATATCTTCAGCGACGGTTCTATCTGGCGTCGCCCTTTCAAAGACATAAACTTCGGTCCTATTCGATTCATCGGTGACAGTTCCGTCATCTTCAAAGACATTACTTACGACCAAAACCTCAACCTCTCCCTCCGTTTATACAAACCAAGACTAACAAACCCTACTTCCTCAGCTGCCAAGCTCCCGGTGGTGCTCTACTTTCACGGCGGCGGTTTTTGCGTCGGCTCACGTGTCTGGCCCCACTTCCGCAACGTATGTGTCCGATTGGCAGCCGAGCTCAACGCCCTTGTGGTGTCACCGGACCACCGGCTTGCGCCGGAGGATAAGCTCCCTGCTGCGCTGGACGATGCGGCGTGTGTGGTGGATTGGCTGCAAAGGGAGGCCTTGATGAGTGAGAGTGGCGGTGACTGTGATGCATGGATACGTGGTGGTGTCGTTGACTTTGATAGGGTTTTTATCGTGGGTGATTCTTCCGGTGGCACCATAGCTCACCACCTGGCGGTTTCGCTCGGAGGCGGATCAGTCAAGGCGGCGCCGCTTCGGGTACGGGGGTACGTGTTGGTGGCGCCATTTTTTGGTGGAGTGGAGTGGACAAAATCAGAGCAGGAGCCTCATGAGCCAAGGCTGACATTGGATGTAGTTGAAAGACTTTGGAGGCTTTCCCTGCCAGAAGGAGCAACCAGGGATCATCCGCTGGCAAACCCATTTGGACCAAACAGTCCACGCCTTGAGACGGTGGATCTTGATCCCATCTTGGTTATTGTGGGTGGCGATGAAATGTTGAAAGATAGATCGGTGATGTACTGGAGTAAACTAAAAGAGTTGGGAAAGAAAATTGAATATGCGGAATTTGATGGAGAGCAGCATGGGTTTTTCTCAATTAATCCATACTCAGATGTTGCAAACGAAGCATTCCACGTTGTTAAAAGATTTGTGATTGAAAACTCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

333

Amino Acids

36.79

Weight (kDa)

5.77

Isoelectric Point (pI)

39.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 68 - 184 4.4e-10 BD-FAE
Abhydrolase_3 PF07859 79 - 307 1.1e-52 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 79 - 241 7e-06 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 128, 762
AccB1I GGYRCC 3 cut(s) 524, 577, 610
AccB7I CCANNNNNTGG 1 cut(s) 620
AclWI GGATC 4 cut(s) 571, 738, 785, 792
AcsI RAATTY 1 cut(s) 893
AcuI CTGAAG 1 cut(s) 37
AcvI CACGTG 1 cut(s) 275
AcyI GRCGYC 3 cut(s) 73, 578, 611
AfaI GTAC 3 cut(s) 591, 599, 848
AfiI CCNNNNNNNGG 3 cut(s) 544, 620, 778
AflIII ACRYGT 2 cut(s) 274, 600
AgsI TTSAA 6 cut(s) 85, 139, 689, 829, 884, 986
AjnI CCWGG 2 cut(s) 540, 725
AluBI AGCT 5 cut(s) 218, 226, 322, 373, 533
AluI AGCT 5 cut(s) 218, 226, 322, 373, 533
Alw21I GWGCWC 2 cut(s) 240, 324
Alw26I GTCTC 1 cut(s) 770
AlwI GGATC 4 cut(s) 571, 738, 785, 792
AoxI GGCC 2 cut(s) 281, 426
ApeKI GCWGC 5 cut(s) 218, 314, 380, 415, 907
ApoI RAATTY 1 cut(s) 893
ArsI GACNNNNNNTTYG 2 cut(s) 80, 112
AseI ATTAAT 1 cut(s) 927
AspLEI GCGC 4 cut(s) 361, 385, 580, 613
AspS9I GGNCC 4 cut(s) 101, 282, 346, 755
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 6 cut(s) 131, 333, 461, 521, 527, 853
AvaII GGWCC 3 cut(s) 101, 346, 755
BaeI ACNNNNGTAYC 2 cut(s) 581, 614
BanI GGYRCC 3 cut(s) 524, 577, 610
BanII GRGCYC 1 cut(s) 324
BbrPI CACGTG 1 cut(s) 275
Bbv12I GWGCWC 2 cut(s) 240, 324
BbvCI CCTCAGC 1 cut(s) 214
BbvI GCAGC 5 cut(s) 205, 326, 367, 402, 919
BccI CCATC 2 cut(s) 803, 893
BceAI ACGGC 1 cut(s) 265
BciT130I CCWGG 2 cut(s) 542, 727
BciVI GTATCC 1 cut(s) 459
BcnI CCSGG 1 cut(s) 230
BcoDI GTCTC 1 cut(s) 770
BfoI RGCGCY 2 cut(s) 581, 614
BfuI GTATCC 1 cut(s) 459
BisI GCNGC 9 cut(s) 219, 253, 315, 381, 396, 416, 576, 581, 908
BlsI GCNGC 9 cut(s) 220, 254, 316, 382, 397, 417, 577, 582, 909
Bme1390I CCNGG 3 cut(s) 230, 542, 727
Bme18I GGWCC 3 cut(s) 101, 346, 755
BmgT120I GGNCC 4 cut(s) 101, 282, 346, 755
BmiI GGNNCC 6 cut(s) 7, 284, 526, 579, 612, 654
BmrFI CCNGG 3 cut(s) 230, 542, 727
BmsI GCATC 2 cut(s) 382, 448
BpmI CTGGAG 1 cut(s) 871
Bpu10I CCTNAGC 1 cut(s) 214
BpuEI CTTGAG 1 cut(s) 794
BpuMI CCSGG 1 cut(s) 230
BsaAI YACGTR 3 cut(s) 275, 470, 601
BsaBI GATNNNNATC 1 cut(s) 789
BsaHI GRCGYC 3 cut(s) 73, 578, 611
BsaJI CCNNGG 3 cut(s) 31, 665, 726
BsaWI WCCGGW 2 cut(s) 343, 518
Bsc4I CCNNNNNNNGG 3 cut(s) 544, 620, 778
Bse118I RCCGGY 1 cut(s) 351
Bse1I ACTGG 1 cut(s) 854
Bse8I GATNNNNATC 1 cut(s) 789
BseBI CCWGG 2 cut(s) 542, 727
BseDI CCNNGG 3 cut(s) 31, 665, 726
BseGI GGATG 2 cut(s) 685, 733
BseJI GATNNNNATC 1 cut(s) 789
BseLI CCNNNNNNNGG 3 cut(s) 544, 620, 778
BseMII CTCAG 2 cut(s) 228, 951
BseNI ACTGG 1 cut(s) 854
BseXI GCAGC 5 cut(s) 205, 326, 367, 402, 919
BshFI GGCC 2 cut(s) 283, 428
BshNI GGYRCC 3 cut(s) 524, 577, 610
BsiHKAI GWGCWC 2 cut(s) 240, 324
BsiSI CCGG 5 cut(s) 230, 344, 352, 362, 519
BslI CCNNNNNNNGG 3 cut(s) 544, 620, 778
BsmAI GTCTC 1 cut(s) 770
BsmBI CGTCTC 1 cut(s) 770
BsmI GAATGC 1 cut(s) 956
BsnI GGCC 2 cut(s) 283, 428
Bsp1286I GDGCHC 2 cut(s) 240, 324
Bsp143I GATC 5 cut(s) 563, 730, 784, 790, 836
BspANI GGCC 2 cut(s) 283, 428
BspCNI CTCAG 2 cut(s) 227, 950
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 6 cut(s) 7, 284, 526, 579, 612, 654
BspPI GGATC 4 cut(s) 571, 738, 785, 792
BspT107I GGYRCC 3 cut(s) 524, 577, 610
BsrFI RCCGGY 1 cut(s) 351
BsrI ACTGG 1 cut(s) 854
BssAI RCCGGY 1 cut(s) 351
BssECI CCNNGG 3 cut(s) 31, 665, 726
BssMI GATC 5 cut(s) 563, 730, 784, 790, 836
BssNI GRCGYC 3 cut(s) 73, 578, 611
BssT1I CCWWGG 1 cut(s) 665
Bst2UI CCWGG 2 cut(s) 542, 727
Bst4CI ACNGT 5 cut(s) 62, 125, 455, 764, 781
BstACI GRCGYC 3 cut(s) 73, 578, 611
BstBAI YACGTR 3 cut(s) 275, 470, 601
BstC8I GCNNGC 2 cut(s) 357, 741
BstDEI CTNAG 2 cut(s) 214, 937
BstF5I GGATG 2 cut(s) 685, 733
BstH2I RGCGCY 2 cut(s) 581, 614
BstHHI GCGC 4 cut(s) 361, 385, 580, 613
BstKTI GATC 5 cut(s) 566, 733, 787, 793, 839
BstMAI GTCTC 1 cut(s) 770
BstMBI GATC 5 cut(s) 563, 730, 784, 790, 836
BstMWI GCNNNNNNNGC 4 cut(s) 261, 661, 709, 953
BstNI CCWGG 2 cut(s) 542, 727
BstSCI CCNGG 3 cut(s) 228, 540, 725
BstV1I GCAGC 5 cut(s) 205, 326, 367, 402, 919
BstX2I RGATCY 1 cut(s) 784
BstYI RGATCY 1 cut(s) 784
BsuI GTATCC 1 cut(s) 459
BsuRI GGCC 2 cut(s) 283, 428
BtgZI GCGATG 1 cut(s) 831
BtsCI GGATG 2 cut(s) 685, 733
Cac8I GCNNGC 2 cut(s) 357, 741
CciI TCATGA 1 cut(s) 658
CfoI GCGC 4 cut(s) 361, 385, 580, 613
Cfr10I RCCGGY 1 cut(s) 351
Cfr13I GGNCC 4 cut(s) 101, 282, 346, 755
CseI GACGC 2 cut(s) 62, 253
Csp6I GTAC 3 cut(s) 590, 598, 847
CviAII CATG 3 cut(s) 462, 659, 911
CviQI GTAC 3 cut(s) 590, 598, 847
DdeI CTNAG 2 cut(s) 214, 937
DinI GGCGCC 2 cut(s) 579, 612
DpnI GATC 5 cut(s) 565, 732, 786, 792, 838
DpnII GATC 5 cut(s) 563, 730, 784, 790, 836
DrdI GACNNNNNNGTC 2 cut(s) 128, 762
DseDI GACNNNNNNGTC 2 cut(s) 128, 762
EciI GGCGGA 1 cut(s) 576
Ecl136II GAGCTC 1 cut(s) 322
Eco130I CCWWGG 1 cut(s) 665
Eco147I AGGCCT 1 cut(s) 428
Eco24I GRGCYC 1 cut(s) 324
Eco47I GGWCC 3 cut(s) 101, 346, 755
Eco53kI GAGCTC 1 cut(s) 322
Eco57I CTGAAG 1 cut(s) 37
Eco72I CACGTG 1 cut(s) 275
EcoICRI GAGCTC 1 cut(s) 322
EcoRII CCWGG 2 cut(s) 540, 725
EcoT14I CCWWGG 1 cut(s) 665
EcoT22I ATGCAT 1 cut(s) 463
EcoT38I GRGCYC 1 cut(s) 324
EgeI GGCGCC 2 cut(s) 579, 612
EheI GGCGCC 2 cut(s) 579, 612
ErhI CCWWGG 1 cut(s) 665
Esp3I CGTCTC 1 cut(s) 770
FaeI CATG 3 cut(s) 465, 662, 914
FatI CATG 3 cut(s) 461, 658, 910
Fnu4HI GCNGC 9 cut(s) 219, 253, 315, 381, 396, 416, 576, 581, 908
FokI GGATG 2 cut(s) 692, 720
FriOI GRGCYC 1 cut(s) 324
Fsp4HI GCNGC 9 cut(s) 219, 253, 315, 381, 396, 416, 576, 581, 908
GlaI GCGC 4 cut(s) 360, 384, 579, 612
GluI GCNGC 9 cut(s) 219, 253, 315, 381, 396, 416, 576, 581, 908
GsuI CTGGAG 1 cut(s) 871
HaeII RGCGCY 2 cut(s) 581, 614
HaeIII GGCC 2 cut(s) 283, 428
HapII CCGG 5 cut(s) 230, 344, 352, 362, 519
HgaI GACGC 2 cut(s) 62, 253
HhaI GCGC 4 cut(s) 361, 385, 580, 613
Hin1I GRCGYC 3 cut(s) 73, 578, 611
Hin1II CATG 3 cut(s) 465, 662, 914
Hin6I GCGC 4 cut(s) 359, 383, 578, 611
HinP1I GCGC 4 cut(s) 359, 383, 578, 611
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HinfI GANTC 2 cut(s) 111, 512
HpaII CCGG 5 cut(s) 230, 344, 352, 362, 519
HphI GGTGA 6 cut(s) 131, 333, 461, 521, 527, 853
Hpy166II GTNNAC 4 cut(s) 484, 636, 767, 857
Hpy188I TCNGA 4 cut(s) 308, 557, 646, 940
Hpy188III TCNNGA 3 cut(s) 9, 659, 788
Hpy8I GTNNAC 4 cut(s) 484, 636, 767, 857
Hpy99I CGWCG 3 cut(s) 62, 78, 269
HpyAV CCTTC 1 cut(s) 710
HpyCH4III ACNGT 5 cut(s) 62, 125, 455, 764, 781
HpyCH4IV ACGT 5 cut(s) 274, 297, 469, 600, 963
HpyCH4V TGCA 3 cut(s) 418, 461, 947
HpyF10VI GCNNNNNNNGC 4 cut(s) 261, 661, 709, 953
HpyF3I CTNAG 2 cut(s) 214, 937
HpySE526I ACGT 5 cut(s) 274, 297, 469, 600, 963
Hsp92I GRCGYC 3 cut(s) 73, 578, 611
Hsp92II CATG 3 cut(s) 465, 662, 914
HspAI GCGC 4 cut(s) 359, 383, 578, 611
KasI GGCGCC 2 cut(s) 577, 610
Kzo9I GATC 5 cut(s) 563, 730, 784, 790, 836
LmnI GCTCC 4 cut(s) 231, 378, 652, 719
Lsp1109I GCAGC 5 cut(s) 205, 326, 367, 402, 919
LweI GCATC 2 cut(s) 382, 448
MaeII ACGT 5 cut(s) 274, 297, 469, 600, 963
MaeIII GTNAC 3 cut(s) 119, 339, 449
MalI GATC 5 cut(s) 565, 732, 786, 792, 838
MboI GATC 5 cut(s) 563, 730, 784, 790, 836
MboII GAAGA 3 cut(s) 43, 127, 507
MflI RGATCY 1 cut(s) 784
MhlI GDGCHC 2 cut(s) 240, 324
MluCI AATT 3 cut(s) 879, 893, 924
Mly113I GGCGCC 2 cut(s) 578, 611
Mph1103I ATGCAT 1 cut(s) 463
MseI TTAA 2 cut(s) 927, 969
MspA1I CMGCKG 2 cut(s) 218, 739
MspI CCGG 5 cut(s) 230, 344, 352, 362, 519
MspR9I CCNGG 3 cut(s) 230, 542, 727
Mva1269I GAATGC 1 cut(s) 956
MvaI CCWGG 2 cut(s) 542, 727
MwoI GCNNNNNNNGC 4 cut(s) 261, 661, 709, 953
NarI GGCGCC 2 cut(s) 578, 611
NciI CCSGG 1 cut(s) 230
NdeII GATC 5 cut(s) 563, 730, 784, 790, 836
NlaIII CATG 3 cut(s) 465, 662, 914
NlaIV GGNNCC 6 cut(s) 7, 284, 526, 579, 612, 654
NmeAIII GCCGAG 1 cut(s) 343
NmuCI GTSAC 3 cut(s) 119, 339, 449
NsiI ATGCAT 1 cut(s) 463
PagI TCATGA 1 cut(s) 658
PceI AGGCCT 1 cut(s) 428
PctI GAATGC 1 cut(s) 956
PfeI GAWTC 2 cut(s) 111, 512
PflMI CCANNNNNTGG 1 cut(s) 620
PkrI GCNGC 9 cut(s) 220, 254, 316, 382, 397, 417, 577, 582, 909
PluTI GGCGCC 2 cut(s) 581, 614
PmaCI CACGTG 1 cut(s) 275
PmlI CACGTG 1 cut(s) 275
Ppu21I YACGTR 3 cut(s) 275, 470, 601
PshBI ATTAAT 1 cut(s) 927
Psp124BI GAGCTC 1 cut(s) 324
Psp6I CCWGG 2 cut(s) 540, 725
PspCI CACGTG 1 cut(s) 275
PspGI CCWGG 2 cut(s) 540, 725
PspN4I GGNNCC 6 cut(s) 7, 284, 526, 579, 612, 654
PspPI GGNCC 4 cut(s) 101, 282, 346, 755
PsuI RGATCY 1 cut(s) 784
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 3 cut(s) 591, 599, 848
RsaNI GTAC 3 cut(s) 590, 598, 847
SacI GAGCTC 1 cut(s) 324
SaqAI TTAA 2 cut(s) 927, 969
SatI GCNGC 9 cut(s) 219, 253, 315, 381, 396, 416, 576, 581, 908
Sau3AI GATC 5 cut(s) 563, 730, 784, 790, 836
Sau96I GGNCC 4 cut(s) 101, 282, 346, 755
ScrFI CCNGG 3 cut(s) 230, 542, 727
SduI GDGCHC 2 cut(s) 240, 324
SfaNI GCATC 2 cut(s) 382, 448
SfoI GGCGCC 2 cut(s) 579, 612
SinI GGWCC 3 cut(s) 101, 346, 755
SmlI CTYRAG 1 cut(s) 773
SmoI CTYRAG 1 cut(s) 773
Sse9I AATT 3 cut(s) 879, 893, 924
SseBI AGGCCT 1 cut(s) 428
SspDI GGCGCC 2 cut(s) 577, 610
SstI GAGCTC 1 cut(s) 324
StuI AGGCCT 1 cut(s) 428
StyD4I CCNGG 3 cut(s) 228, 540, 725
StyI CCWWGG 1 cut(s) 665
TaaI ACNGT 5 cut(s) 62, 125, 455, 764, 781
TaiI ACGT 5 cut(s) 277, 300, 472, 603, 966
TaqI TCGA 1 cut(s) 109
TaqII GACCGA 1 cut(s) 89
TasI AATT 3 cut(s) 879, 893, 924
TatI WGTACW 1 cut(s) 846
TauI GCSGC 4 cut(s) 255, 398, 578, 583
TfiI GAWTC 2 cut(s) 111, 512
Tru1I TTAA 2 cut(s) 927, 969
Tru9I TTAA 2 cut(s) 927, 969
TseFI GTSAC 3 cut(s) 119, 339, 449
TseI GCWGC 5 cut(s) 218, 314, 380, 415, 907
Tsp45I GTSAC 3 cut(s) 119, 339, 449
TspDTI ATGAA 2 cut(s) 103, 834
TspGWI ACGGA 2 cut(s) 118, 167
Van91I CCANNNNNTGG 1 cut(s) 620
VpaK11BI GGWCC 3 cut(s) 101, 346, 755
VspI ATTAAT 1 cut(s) 927
XapI RAATTY 1 cut(s) 893
XcmI CCANNNNNNNNNTGG 1 cut(s) 229
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.