MD14G1045500.v1.1

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001599
Physical Location & Seq
Reverse (-)
4311213 .. 4315061
3849 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1045500.v1.1.491

Sequence Viewer

Length: 975 bp
ATGGGTTCCCTCCCTCACATAGTAGAAGATTGCATGGGAGTTCTCCAAATCTTTAGCGACGGTTCGATCAAACGCTGCTCCTTAACGGACATAAACGTCAACATTCCTGTCATTGACGACGGCTCCGTGGCCTTCAAAGACATAATTTTCGACACAAAACATAAACTTTCCCTCCGTTTATACAAACCCAAATCAGCAAACCTCAATTCCAAGCTCCCCGTGGTCTTCTACTTCCACGGTGGCGGCTTCTGCGTTGGCTCGCACGTGTGGCCAAATTTCCACAACTGCTGCATCCGCCTATCTTCCGGGCTCCAGTCACTTGTCGTGGCCCCGGACTACAGGCTCGCTCCAGAGCATAAACTTCCGGCTGCGATTGATGATGCAGTGAGTGCAGTGGAGTGGCTGCAAAAGGAGGCATTGGGTGAGAGCACGAATGATGCATGGATTGGTGGTACGGCTGACTTGGATCGGGTTTTTGTATTGGGTGACTCTTCAGGTGGGAATATGGCCCACCACCTGGCGGTTCGGCTTGGGATGGGGTCTGTCAAGGCGGCTCCGGTTAGGGTACGGGGGTATGTGCTCCTAGCGCCATTTTTTGGTGGGGTGGAGAGGACAAGGTCAGAGGAAGGTCCTTGTGAGGCGCTTCTAAGTTTGGAAATACTTGACAGATTTTGGAGGCTTTCAATGCCAACAGAAGAAACCAGAGACAATCCAATGGTCAACCCGTTTGGACCGAACAGTCCAAACCTTGAGAAGGTGGCCCTTGATCCCATCTTGGTTGTCGTGGGCGGCAATGAATTGTTGAAAGATAGGGTGGAGAGTTACTGGAAAAAGTTAAAAGATTTGGGAAAGAAAATTGAGTATGTCGAATTTGAAGGACAGGAACATGGGGTTTTAACGAATGATCCATACTCAGAAGTCTCAAATGAAGCTTTGCAAGTTATTAAAAGATTTATGACTGAAAACTGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

325

Amino Acids

35.77

Weight (kDa)

5.52

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 56 - 174 1e-06 Carboxylesterase family
BD-FAE PF20434 60 - 175 1.4e-11 BD-FAE
Abhydrolase_3 PF07859 75 - 299 5.3e-56 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 95, 738
AccB7I CCANNNNNTGG 2 cut(s) 517, 596
AciI CCGC 5 cut(s) 243, 295, 521, 551, 789
AclWI GGATC 3 cut(s) 474, 761, 899
AcoI YGGCCR 1 cut(s) 269
AcsI RAATTY 2 cut(s) 274, 869
AcuI CTGAAG 1 cut(s) 477
AcvI CACGTG 1 cut(s) 265
AfaI GTAC 2 cut(s) 454, 567
AfiI CCNNNNNNNGG 4 cut(s) 517, 520, 596, 754
AflIII ACRYGT 1 cut(s) 264
AgsI TTSAA 4 cut(s) 136, 684, 805, 875
AjnI CCWGG 1 cut(s) 516
AluBI AGCT 2 cut(s) 214, 932
AluI AGCT 2 cut(s) 214, 932
Alw21I GWGCWC 2 cut(s) 431, 582
Alw26I GTCTC 2 cut(s) 699, 925
AlwI GGATC 3 cut(s) 474, 761, 899
AoxI GGCC 5 cut(s) 129, 269, 327, 507, 759
ApeKI GCWGC 4 cut(s) 75, 288, 368, 403
ApoI RAATTY 2 cut(s) 274, 869
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AspLEI GCGC 2 cut(s) 589, 643
AspS9I GGNCC 5 cut(s) 328, 508, 629, 731, 760
AsuC2I CCSGG 2 cut(s) 307, 332
AsuHPI GGTGA 2 cut(s) 434, 497
AvaII GGWCC 2 cut(s) 629, 731
BaeI ACNNNNGTAYC 2 cut(s) 557, 590
BalI TGGCCA 1 cut(s) 271
BanII GRGCYC 1 cut(s) 312
BbrPI CACGTG 1 cut(s) 265
BbsI GAAGAC 1 cut(s) 217
Bbv12I GWGCWC 2 cut(s) 431, 582
BbvI GCAGC 4 cut(s) 62, 275, 355, 390
BccI CCATC 2 cut(s) 529, 779
BceAI ACGGC 2 cut(s) 136, 471
BciT130I CCWGG 1 cut(s) 518
BcnI CCSGG 2 cut(s) 307, 332
BcoDI GTCTC 2 cut(s) 699, 925
BfaI CTAG 1 cut(s) 584
BfmI CTRYAG 1 cut(s) 337
BfoI RGCGCY 2 cut(s) 590, 644
BisI GCNGC 7 cut(s) 76, 244, 289, 369, 404, 552, 790
BlsI GCNGC 7 cut(s) 77, 245, 290, 370, 405, 553, 791
Bme1390I CCNGG 3 cut(s) 307, 332, 518
Bme18I GGWCC 2 cut(s) 629, 731
BmgT120I GGNCC 5 cut(s) 328, 508, 629, 731, 760
BmiI GGNNCC 5 cut(s) 7, 124, 311, 330, 555
BmrFI CCNGG 3 cut(s) 307, 332, 518
BmsI GCATC 3 cut(s) 300, 370, 427
BpiI GAAGAC 1 cut(s) 217
BpmI CTGGAG 2 cut(s) 296, 333
BpuEI CTTGAG 1 cut(s) 770
BpuMI CCSGG 2 cut(s) 307, 332
BsaAI YACGTR 1 cut(s) 265
BsaJI CCNNGG 4 cut(s) 126, 219, 235, 330
BsaWI WCCGGW 1 cut(s) 556
BsaXI ACNNNNNCTCC 4 cut(s) 107, 137, 156, 186
Bsc4I CCNNNNNNNGG 4 cut(s) 517, 520, 596, 754
Bse1I ACTGG 2 cut(s) 313, 830
Bse3DI GCAATG 1 cut(s) 799
BseBI CCWGG 1 cut(s) 518
BseDI CCNNGG 4 cut(s) 126, 219, 235, 330
BseGI GGATG 2 cut(s) 291, 540
BseLI CCNNNNNNNGG 4 cut(s) 517, 520, 596, 754
BseMI GCAATG 1 cut(s) 799
BseMII CTCAG 1 cut(s) 927
BseNI ACTGG 2 cut(s) 313, 830
BseXI GCAGC 4 cut(s) 62, 275, 355, 390
BsgI GTGCAG 1 cut(s) 411
BshFI GGCC 5 cut(s) 131, 271, 329, 509, 761
BsiHKAI GWGCWC 2 cut(s) 431, 582
BsiSI CCGG 4 cut(s) 306, 332, 365, 557
BslI CCNNNNNNNGG 4 cut(s) 517, 520, 596, 754
BsmAI GTCTC 2 cut(s) 699, 925
BsnI GGCC 5 cut(s) 131, 271, 329, 509, 761
Bsp1286I GDGCHC 3 cut(s) 312, 431, 582
Bsp143I GATC 4 cut(s) 66, 466, 766, 904
BspACI CCGC 5 cut(s) 243, 295, 521, 551, 789
BspANI GGCC 5 cut(s) 131, 271, 329, 509, 761
BspCNI CTCAG 1 cut(s) 926
BspLI GGNNCC 5 cut(s) 7, 124, 311, 330, 555
BspPI GGATC 3 cut(s) 474, 761, 899
BsrDI GCAATG 1 cut(s) 799
BsrI ACTGG 2 cut(s) 313, 830
BssECI CCNNGG 4 cut(s) 126, 219, 235, 330
BssMI GATC 4 cut(s) 66, 466, 766, 904
Bst2UI CCWGG 1 cut(s) 518
Bst4CI ACNGT 3 cut(s) 62, 239, 740
Bst6I CTCTTC 1 cut(s) 496
BstAPI GCANNNNNTGC 1 cut(s) 389
BstBAI YACGTR 1 cut(s) 265
BstC8I GCNNGC 2 cut(s) 260, 345
BstDEI CTNAG 2 cut(s) 647, 913
BstDSI CCRYGG 3 cut(s) 126, 219, 235
BstENI CCTNNNNNAGG 1 cut(s) 752
BstF5I GGATG 2 cut(s) 291, 540
BstH2I RGCGCY 2 cut(s) 590, 644
BstHHI GCGC 2 cut(s) 589, 643
BstKTI GATC 4 cut(s) 69, 469, 769, 907
BstMAI GTCTC 2 cut(s) 699, 925
BstMBI GATC 4 cut(s) 66, 466, 766, 904
BstMWI GCNNNNNNNGC 6 cut(s) 249, 268, 294, 389, 586, 685
BstNI CCWGG 1 cut(s) 518
BstSCI CCNGG 3 cut(s) 305, 330, 516
BstSFI CTRYAG 1 cut(s) 337
BstV1I GCAGC 4 cut(s) 62, 275, 355, 390
BstV2I GAAGAC 1 cut(s) 217
BsuRI GGCC 5 cut(s) 131, 271, 329, 509, 761
BtgI CCRYGG 3 cut(s) 126, 219, 235
BtsCI GGATG 2 cut(s) 291, 540
BtsI GCAGTG 2 cut(s) 390, 399
BtsIMutI CAGTG 2 cut(s) 390, 399
Cac8I GCNNGC 2 cut(s) 260, 345
CfoI GCGC 2 cut(s) 589, 643
Cfr13I GGNCC 5 cut(s) 328, 508, 629, 731, 760
Csp6I GTAC 2 cut(s) 453, 566
CviAII CATG 3 cut(s) 34, 441, 887
CviQI GTAC 2 cut(s) 453, 566
DdeI CTNAG 2 cut(s) 647, 913
DpnI GATC 4 cut(s) 68, 468, 768, 906
DpnII GATC 4 cut(s) 66, 466, 766, 904
DrdI GACNNNNNNGTC 2 cut(s) 95, 738
DseDI GACNNNNNNGTC 2 cut(s) 95, 738
EaeI YGGCCR 1 cut(s) 269
Eam1104I CTCTTC 1 cut(s) 496
EarI CTCTTC 1 cut(s) 496
EciI GGCGGA 1 cut(s) 284
Eco24I GRGCYC 1 cut(s) 312
Eco47I GGWCC 2 cut(s) 629, 731
Eco57I CTGAAG 1 cut(s) 477
Eco72I CACGTG 1 cut(s) 265
EcoNI CCTNNNNNAGG 1 cut(s) 752
EcoO109I RGGNCCY 1 cut(s) 629
EcoRII CCWGG 1 cut(s) 516
EcoT22I ATGCAT 1 cut(s) 442
EcoT38I GRGCYC 1 cut(s) 312
FaeI CATG 3 cut(s) 37, 444, 890
FatI CATG 3 cut(s) 33, 440, 886
Fnu4HI GCNGC 7 cut(s) 76, 244, 289, 369, 404, 552, 790
FokI GGATG 2 cut(s) 278, 547
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 7 cut(s) 76, 244, 289, 369, 404, 552, 790
FspBI CTAG 1 cut(s) 584
GlaI GCGC 2 cut(s) 588, 642
GluI GCNGC 7 cut(s) 76, 244, 289, 369, 404, 552, 790
GsuI CTGGAG 2 cut(s) 296, 333
HaeII RGCGCY 2 cut(s) 590, 644
HaeIII GGCC 5 cut(s) 131, 271, 329, 509, 761
HapII CCGG 4 cut(s) 306, 332, 365, 557
HhaI GCGC 2 cut(s) 589, 643
Hin1II CATG 3 cut(s) 37, 444, 890
Hin6I GCGC 2 cut(s) 587, 641
HinP1I GCGC 2 cut(s) 587, 641
HincII GTYRAC 2 cut(s) 100, 721
HindII GTYRAC 2 cut(s) 100, 721
HindIII AAGCTT 1 cut(s) 930
HinfI GANTC 1 cut(s) 488
HpaII CCGG 4 cut(s) 306, 332, 365, 557
HphI GGTGA 2 cut(s) 434, 497
Hpy166II GTNNAC 2 cut(s) 100, 721
Hpy188I TCNGA 2 cut(s) 622, 916
Hpy188III TCNNGA 1 cut(s) 350
Hpy8I GTNNAC 2 cut(s) 100, 721
Hpy99I CGWCG 2 cut(s) 62, 122
HpyAV CCTTC 4 cut(s) 142, 620, 748, 869
HpyCH4III ACNGT 3 cut(s) 62, 239, 740
HpyCH4IV ACGT 2 cut(s) 96, 264
HpyCH4V TGCA 8 cut(s) 33, 291, 383, 392, 406, 440, 937, 969
HpyF10VI GCNNNNNNNGC 6 cut(s) 249, 268, 294, 389, 586, 685
HpyF3I CTNAG 2 cut(s) 647, 913
HpySE526I ACGT 2 cut(s) 96, 264
Hsp92II CATG 3 cut(s) 37, 444, 890
HspAI GCGC 2 cut(s) 587, 641
Kzo9I GATC 4 cut(s) 66, 466, 766, 904
LmnI GCTCC 7 cut(s) 83, 128, 219, 315, 352, 559, 585
Lsp1109I GCAGC 4 cut(s) 62, 275, 355, 390
LweI GCATC 3 cut(s) 300, 370, 427
MaeI CTAG 1 cut(s) 584
MaeII ACGT 2 cut(s) 96, 264
MaeIII GTNAC 3 cut(s) 315, 485, 821
MalI GATC 4 cut(s) 68, 468, 768, 906
MboI GATC 4 cut(s) 66, 466, 766, 904
MboII GAAGA 5 cut(s) 38, 217, 294, 483, 707
MhlI GDGCHC 3 cut(s) 312, 431, 582
MlsI TGGCCA 1 cut(s) 271
MluCI AATT 6 cut(s) 144, 205, 274, 797, 855, 869
MluNI TGGCCA 1 cut(s) 271
MlyI GAGTC 1 cut(s) 482
MnlI CCTC 9 cut(s) 20, 24, 182, 212, 406, 603, 616, 631, 669
Mox20I TGGCCA 1 cut(s) 271
Mph1103I ATGCAT 1 cut(s) 442
MscI TGGCCA 1 cut(s) 271
MseI TTAA 4 cut(s) 83, 836, 896, 945
Msp20I TGGCCA 1 cut(s) 271
MspI CCGG 4 cut(s) 306, 332, 365, 557
MspR9I CCNGG 3 cut(s) 307, 332, 518
MvaI CCWGG 1 cut(s) 518
MwoI GCNNNNNNNGC 6 cut(s) 249, 268, 294, 389, 586, 685
NciI CCSGG 2 cut(s) 307, 332
NdeII GATC 4 cut(s) 66, 466, 766, 904
NlaIII CATG 3 cut(s) 37, 444, 890
NlaIV GGNNCC 5 cut(s) 7, 124, 311, 330, 555
NmuCI GTSAC 2 cut(s) 315, 485
NsiI ATGCAT 1 cut(s) 442
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PflFI GACNNNGTC 1 cut(s) 616
PflMI CCANNNNNTGG 2 cut(s) 517, 596
PkrI GCNGC 7 cut(s) 77, 245, 290, 370, 405, 553, 791
PleI GAGTC 1 cut(s) 482
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
PpsI GAGTC 1 cut(s) 482
Ppu21I YACGTR 1 cut(s) 265
PpuMI RGGWCCY 1 cut(s) 629
Psp5II RGGWCCY 1 cut(s) 629
Psp6I CCWGG 1 cut(s) 516
PspCI CACGTG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 516
PspN4I GGNNCC 5 cut(s) 7, 124, 311, 330, 555
PspPI GGNCC 5 cut(s) 328, 508, 629, 731, 760
PspPPI RGGWCCY 1 cut(s) 629
PsyI GACNNNGTC 1 cut(s) 616
RsaI GTAC 2 cut(s) 454, 567
RsaNI GTAC 2 cut(s) 453, 566
SaqAI TTAA 4 cut(s) 83, 836, 896, 945
SatI GCNGC 7 cut(s) 76, 244, 289, 369, 404, 552, 790
Sau3AI GATC 4 cut(s) 66, 466, 766, 904
Sau96I GGNCC 5 cut(s) 328, 508, 629, 731, 760
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 3 cut(s) 307, 332, 518
SduI GDGCHC 3 cut(s) 312, 431, 582
SfaNI GCATC 3 cut(s) 300, 370, 427
SfcI CTRYAG 1 cut(s) 337
SinI GGWCC 2 cut(s) 629, 731
SmlI CTYRAG 1 cut(s) 749
SmoI CTYRAG 1 cut(s) 749
Sse9I AATT 6 cut(s) 144, 205, 274, 797, 855, 869
SsiI CCGC 5 cut(s) 243, 295, 521, 551, 789
SspMI CTAG 1 cut(s) 584
StyD4I CCNGG 3 cut(s) 305, 330, 516
TaaI ACNGT 3 cut(s) 62, 239, 740
TaiI ACGT 2 cut(s) 99, 267
TaqI TCGA 3 cut(s) 65, 150, 867
TaqII GACCGA 1 cut(s) 748
TasI AATT 6 cut(s) 144, 205, 274, 797, 855, 869
TauI GCSGC 3 cut(s) 246, 554, 792
Tru1I TTAA 4 cut(s) 83, 836, 896, 945
Tru9I TTAA 4 cut(s) 83, 836, 896, 945
TscAI CASTG 2 cut(s) 390, 399
TseFI GTSAC 2 cut(s) 315, 485
TseI GCWGC 4 cut(s) 75, 288, 368, 403
Tsp45I GTSAC 2 cut(s) 315, 485
TspDTI ATGAA 2 cut(s) 810, 942
TspGWI ACGGA 3 cut(s) 101, 115, 164
TspRI CASTG 2 cut(s) 390, 399
Tth111I GACNNNGTC 1 cut(s) 616
Van91I CCANNNNNTGG 2 cut(s) 517, 596
VpaK11BI GGWCC 2 cut(s) 629, 731
XagI CCTNNNNNAGG 1 cut(s) 752
XapI RAATTY 2 cut(s) 274, 869
XcmI CCANNNNNNNNNTGG 1 cut(s) 217
XspI CTAG 1 cut(s) 584
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.