Rh3AG211700

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001599
Physical Location & Seq
Reverse (-)
21338389 .. 21341785
3397 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG211700.1

Sequence Viewer

Length: 720 bp
ATGGGTTCCCTCGCTCACATTGTGGAAGACTGCATGGGCGTTCTCCAACTCTTTAGCGACGGTTCCGTCCATCGCTGCTCTTTGAAAGACATAAACTTCCCGTTTCCAGCCGTTAACGATAACTCCGTGGACTTCAAAGACGTCGTTTTCGACAGAAAGCTCAACATTTCCCTACGTGTGTACAGACCTAAAAGCCATGCGTCCCCGACGCCCGCAAAACTCCCCGTGGTGTTTTACTTCCACGGGGGCGGTTTCTGCGTTGGCTCGCGGGTGTGGCCCAACTGCCACAACTGCTGCATCCGCCTGGCATCTGAGCTCAACGCGCTCGTCGTGGCGCCAGACTACCGGCTCGCCCCCGAGCACAAGCTCCCGGCCGCGGTGGATGACGCGGTGAGTGCGGTGGAGTGGCTGCAGAGGGAGGCTTTGATGAGTGAGGGCAGTGGTGATTGTGATGCATGGCTGGGTGGTGGTATGGTTGACTTTGACCGGGTTTATGTGGTGGGCGATTCATCAGGAGGTAATATGGCTCACCACGTGGCCGTACGGCTCGGAGCCGGGTCGGTCAAGGCGGCGCCGGTGCGGGTACGCGGATATGTGCTTTTGGCGCCATTTTTTGGTGGAGTGGAGAGGACAAAGTCGGAGGAGGGTCCGTCGGAGACGTTTTTGAATTTGGAGATACTTGACAGGGGAAATCGGGGGAACCAGAAGATGCACCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

239

Amino Acids

26.0

Weight (kDa)

6.3

Isoelectric Point (pI)

46.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 58 - 119 3.5e-07 Carboxylesterase family
BD-FAE PF20434 60 - 181 2.4e-10 BD-FAE
Abhydrolase_3 PF07859 77 - 217 2.5e-38 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 65
AatII GACGTC 1 cut(s) 144
AccB1I GGYRCC 3 cut(s) 334, 571, 604
AccB7I CCANNNNNTGG 1 cut(s) 614
AccII CGCG 5 cut(s) 268, 323, 377, 389, 588
AcoI YGGCCR 2 cut(s) 372, 537
AcsI RAATTY 1 cut(s) 667
AcvI CACGTG 1 cut(s) 535
AcyI GRCGYC 5 cut(s) 141, 209, 335, 572, 605
AdeI CACNNNGTG 2 cut(s) 22, 535
AfaI GTAC 3 cut(s) 182, 543, 585
AfiI CCNNNNNNNGG 2 cut(s) 376, 614
AflIII ACRYGT 1 cut(s) 175
AgsI TTSAA 3 cut(s) 85, 136, 667
AjnI CCWGG 1 cut(s) 303
AluBI AGCT 3 cut(s) 160, 316, 367
AluI AGCT 3 cut(s) 160, 316, 367
Alw21I GWGCWC 2 cut(s) 318, 363
Alw26I GTCTC 1 cut(s) 650
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 3 cut(s) 275, 372, 537
ApeKI GCWGC 3 cut(s) 75, 294, 409
ApoI RAATTY 1 cut(s) 667
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AspLEI GCGC 4 cut(s) 325, 337, 574, 607
AspS9I GGNCC 2 cut(s) 276, 647
AsuC2I CCSGG 3 cut(s) 371, 488, 556
AsuHPI GGTGA 3 cut(s) 403, 455, 521
AvaI CYCGRG 1 cut(s) 356
AvaII GGWCC 1 cut(s) 647
BanI GGYRCC 3 cut(s) 334, 571, 604
BanII GRGCYC 1 cut(s) 318
BbrPI CACGTG 1 cut(s) 535
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 2 cut(s) 318, 363
BbvI GCAGC 3 cut(s) 62, 281, 396
BccI CCATC 1 cut(s) 78
BceAI ACGGC 3 cut(s) 95, 524, 560
BciT130I CCWGG 1 cut(s) 305
BcnI CCSGG 3 cut(s) 371, 488, 556
BcoDI GTCTC 1 cut(s) 650
BfmI CTRYAG 1 cut(s) 410
BfoI RGCGCY 3 cut(s) 338, 575, 608
BisI GCNGC 5 cut(s) 76, 295, 375, 410, 570
BlsI GCNGC 5 cut(s) 77, 296, 376, 411, 571
Bme1390I CCNGG 4 cut(s) 305, 371, 488, 556
Bme18I GGWCC 1 cut(s) 647
BmeT110I CYCGRG 1 cut(s) 356
BmgT120I GGNCC 2 cut(s) 276, 647
BmiI GGNNCC 8 cut(s) 7, 64, 336, 553, 573, 606, 648, 701
BmrFI CCNGG 4 cut(s) 305, 371, 488, 556
BmsI GCATC 4 cut(s) 306, 317, 442, 699
BpiI GAAGAC 1 cut(s) 33
BpuMI CCSGG 3 cut(s) 371, 488, 556
BsaAI YACGTR 2 cut(s) 176, 535
BsaHI GRCGYC 5 cut(s) 141, 209, 335, 572, 605
BsaJI CCNNGG 4 cut(s) 126, 225, 241, 375
BsaXI ACNNNNNCTCC 4 cut(s) 107, 137, 635, 665
Bsc4I CCNNNNNNNGG 2 cut(s) 376, 614
Bse118I RCCGGY 2 cut(s) 345, 574
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 4 cut(s) 126, 225, 241, 375
BseGI GGATG 2 cut(s) 297, 388
BseLI CCNNNNNNNGG 2 cut(s) 376, 614
BseMII CTCAG 1 cut(s) 303
BseRI GAGGAG 1 cut(s) 656
BseX3I CGGCCG 1 cut(s) 372
BseXI GCAGC 3 cut(s) 62, 281, 396
BseYI CCCAGC 1 cut(s) 460
Bsh1236I CGCG 5 cut(s) 268, 323, 377, 389, 588
Bsh1285I CGRYCG 1 cut(s) 375
BshFI GGCC 3 cut(s) 277, 374, 539
BshNI GGYRCC 3 cut(s) 334, 571, 604
BsiEI CGRYCG 1 cut(s) 375
BsiHKAI GWGCWC 2 cut(s) 318, 363
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 5 cut(s) 346, 371, 487, 555, 575
BsiWI CGTACG 1 cut(s) 541
BslFI GGGAC 1 cut(s) 187
BslI CCNNNNNNNGG 2 cut(s) 376, 614
BsmAI GTCTC 1 cut(s) 650
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 1 cut(s) 187
BsnI GGCC 3 cut(s) 277, 374, 539
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 2 cut(s) 318, 363
Bsp1407I TGTACA 1 cut(s) 180
BspANI GGCC 3 cut(s) 277, 374, 539
BspCNI CTCAG 1 cut(s) 304
BspFNI CGCG 5 cut(s) 268, 323, 377, 389, 588
BspLI GGNNCC 8 cut(s) 7, 64, 336, 553, 573, 606, 648, 701
BspMAI CTGCAG 1 cut(s) 414
BspT107I GGYRCC 3 cut(s) 334, 571, 604
BsrFI RCCGGY 2 cut(s) 345, 574
BsrGI TGTACA 1 cut(s) 180
BssAI RCCGGY 2 cut(s) 345, 574
BssECI CCNNGG 4 cut(s) 126, 225, 241, 375
BssNI GRCGYC 5 cut(s) 141, 209, 335, 572, 605
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 1 cut(s) 62
BstACI GRCGYC 5 cut(s) 141, 209, 335, 572, 605
BstAUI TGTACA 1 cut(s) 180
BstBAI YACGTR 2 cut(s) 176, 535
BstC8I GCNNGC 3 cut(s) 213, 266, 351
BstDEI CTNAG 1 cut(s) 312
BstDSI CCRYGG 4 cut(s) 126, 225, 241, 375
BstF5I GGATG 2 cut(s) 297, 388
BstFNI CGCG 5 cut(s) 268, 323, 377, 389, 588
BstH2I RGCGCY 3 cut(s) 338, 575, 608
BstHHI GCGC 4 cut(s) 325, 337, 574, 607
BstMAI GTCTC 1 cut(s) 650
BstMCI CGRYCG 1 cut(s) 375
BstMWI GCNNNNNNNGC 7 cut(s) 255, 274, 291, 300, 322, 395, 604
BstNI CCWGG 1 cut(s) 305
BstSCI CCNGG 4 cut(s) 303, 369, 486, 554
BstSFI CTRYAG 1 cut(s) 410
BstUI CGCG 5 cut(s) 268, 323, 377, 389, 588
BstV1I GCAGC 3 cut(s) 62, 281, 396
BstV2I GAAGAC 1 cut(s) 33
BstZI CGGCCG 1 cut(s) 372
BsuRI GGCC 3 cut(s) 277, 374, 539
BtgI CCRYGG 4 cut(s) 126, 225, 241, 375
BtgZI GCGATG 1 cut(s) 56
BtsCI GGATG 2 cut(s) 297, 388
BtsI GCAGTG 1 cut(s) 445
BtsIMutI CAGTG 1 cut(s) 445
Cac8I GCNNGC 3 cut(s) 213, 266, 351
CfoI GCGC 4 cut(s) 325, 337, 574, 607
Cfr10I RCCGGY 2 cut(s) 345, 574
Cfr13I GGNCC 2 cut(s) 276, 647
Cfr42I CCGCGG 1 cut(s) 378
CseI GACGC 3 cut(s) 189, 217, 395
Csp6I GTAC 3 cut(s) 181, 542, 584
CviAII CATG 3 cut(s) 34, 197, 456
CviQI GTAC 3 cut(s) 181, 542, 584
DdeI CTNAG 1 cut(s) 312
DinI GGCGCC 3 cut(s) 336, 573, 606
DraIII CACNNNGTG 2 cut(s) 22, 535
DrdI GACNNNNNNGTC 1 cut(s) 65
DseDI GACNNNNNNGTC 1 cut(s) 65
EaeI YGGCCR 2 cut(s) 372, 537
EagI CGGCCG 1 cut(s) 372
EciI GGCGGA 1 cut(s) 290
Ecl136II GAGCTC 1 cut(s) 316
EclXI CGGCCG 1 cut(s) 372
Eco24I GRGCYC 1 cut(s) 318
Eco47I GGWCC 1 cut(s) 647
Eco52I CGGCCG 1 cut(s) 372
Eco53kI GAGCTC 1 cut(s) 316
Eco72I CACGTG 1 cut(s) 535
Eco88I CYCGRG 1 cut(s) 356
EcoICRI GAGCTC 1 cut(s) 316
EcoRII CCWGG 1 cut(s) 303
EcoT22I ATGCAT 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 318
EgeI GGCGCC 3 cut(s) 336, 573, 606
EheI GGCGCC 3 cut(s) 336, 573, 606
Esp3I CGTCTC 1 cut(s) 650
FaeI CATG 3 cut(s) 37, 200, 459
FaiI YATR 8 cut(s) 35, 92, 198, 457, 473, 495, 524, 594
FaqI GGGAC 1 cut(s) 187
FatI CATG 3 cut(s) 33, 196, 455
FauI CCCGC 3 cut(s) 220, 261, 573
Fnu4HI GCNGC 5 cut(s) 76, 295, 375, 410, 570
FokI GGATG 2 cut(s) 284, 395
FriOI GRGCYC 1 cut(s) 318
Fsp4HI GCNGC 5 cut(s) 76, 295, 375, 410, 570
GlaI GCGC 4 cut(s) 324, 336, 573, 606
GluI GCNGC 5 cut(s) 76, 295, 375, 410, 570
GsaI CCCAGC 1 cut(s) 464
HaeII RGCGCY 3 cut(s) 338, 575, 608
HaeIII GGCC 3 cut(s) 277, 374, 539
HapII CCGG 5 cut(s) 346, 371, 487, 555, 575
HgaI GACGC 3 cut(s) 189, 217, 395
HhaI GCGC 4 cut(s) 325, 337, 574, 607
Hin1I GRCGYC 5 cut(s) 141, 209, 335, 572, 605
Hin1II CATG 3 cut(s) 37, 200, 459
Hin6I GCGC 4 cut(s) 323, 335, 572, 605
HinP1I GCGC 4 cut(s) 323, 335, 572, 605
HincII GTYRAC 2 cut(s) 115, 478
HindII GTYRAC 2 cut(s) 115, 478
HinfI GANTC 1 cut(s) 506
HpaI GTTAAC 1 cut(s) 115
HpaII CCGG 5 cut(s) 346, 371, 487, 555, 575
HphI GGTGA 3 cut(s) 403, 455, 521
Hpy166II GTNNAC 4 cut(s) 115, 130, 181, 478
Hpy188I TCNGA 4 cut(s) 313, 551, 640, 655
Hpy188III TCNNGA 1 cut(s) 513
Hpy8I GTNNAC 4 cut(s) 115, 130, 181, 478
Hpy99I CGWCG 5 cut(s) 62, 146, 211, 332, 655
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4IV ACGT 4 cut(s) 141, 175, 534, 659
HpyCH4V TGCA 5 cut(s) 33, 297, 412, 455, 712
HpyF10VI GCNNNNNNNGC 7 cut(s) 255, 274, 291, 300, 322, 395, 604
HpyF3I CTNAG 1 cut(s) 312
HpySE526I ACGT 4 cut(s) 141, 175, 534, 659
Hsp92I GRCGYC 5 cut(s) 141, 209, 335, 572, 605
Hsp92II CATG 3 cut(s) 37, 200, 459
HspAI GCGC 4 cut(s) 323, 335, 572, 605
KasI GGCGCC 3 cut(s) 334, 571, 604
KspAI GTTAAC 1 cut(s) 115
KspI CCGCGG 1 cut(s) 378
LmnI GCTCC 2 cut(s) 372, 551
Lsp1109I GCAGC 3 cut(s) 62, 281, 396
LweI GCATC 4 cut(s) 306, 317, 442, 699
MaeII ACGT 4 cut(s) 141, 175, 534, 659
MboII GAAGA 2 cut(s) 38, 718
MhlI GDGCHC 2 cut(s) 318, 363
MluCI AATT 1 cut(s) 667
Mly113I GGCGCC 3 cut(s) 335, 572, 605
MmeI TCCRAC 3 cut(s) 70, 618, 633
MnlI CCTC 8 cut(s) 20, 408, 412, 427, 509, 621, 634, 637
Mph1103I ATGCAT 1 cut(s) 457
MseI TTAA 1 cut(s) 114
MspA1I CMGCKG 1 cut(s) 377
MspI CCGG 5 cut(s) 346, 371, 487, 555, 575
MspR9I CCNGG 4 cut(s) 305, 371, 488, 556
MvaI CCWGG 1 cut(s) 305
MvnI CGCG 5 cut(s) 268, 323, 377, 389, 588
MwoI GCNNNNNNNGC 7 cut(s) 255, 274, 291, 300, 322, 395, 604
NarI GGCGCC 3 cut(s) 335, 572, 605
NciI CCSGG 3 cut(s) 371, 488, 556
NlaIII CATG 3 cut(s) 37, 200, 459
NlaIV GGNNCC 8 cut(s) 7, 64, 336, 553, 573, 606, 648, 701
NsiI ATGCAT 1 cut(s) 457
PcsI WCGNNNNNNNCGW 3 cut(s) 123, 147, 327
PfeI GAWTC 1 cut(s) 506
Pfl23II CGTACG 1 cut(s) 541
PflFI GACNNNGTC 1 cut(s) 634
PflMI CCANNNNNTGG 1 cut(s) 614
PkrI GCNGC 5 cut(s) 77, 296, 376, 411, 571
PluTI GGCGCC 3 cut(s) 338, 575, 608
PmaCI CACGTG 1 cut(s) 535
PmlI CACGTG 1 cut(s) 535
Ppu21I YACGTR 2 cut(s) 176, 535
Psp124BI GAGCTC 1 cut(s) 318
Psp6I CCWGG 1 cut(s) 303
PspCI CACGTG 1 cut(s) 535
PspFI CCCAGC 1 cut(s) 460
PspGI CCWGG 1 cut(s) 303
PspLI CGTACG 1 cut(s) 541
PspN4I GGNNCC 8 cut(s) 7, 64, 336, 553, 573, 606, 648, 701
PspPI GGNCC 2 cut(s) 276, 647
PstI CTGCAG 1 cut(s) 414
PsyI GACNNNGTC 1 cut(s) 634
RsaI GTAC 3 cut(s) 182, 543, 585
RsaNI GTAC 3 cut(s) 181, 542, 584
SacI GAGCTC 1 cut(s) 318
SacII CCGCGG 1 cut(s) 378
SaqAI TTAA 1 cut(s) 114
SatI GCNGC 5 cut(s) 76, 295, 375, 410, 570
Sau96I GGNCC 2 cut(s) 276, 647
ScrFI CCNGG 4 cut(s) 305, 371, 488, 556
SduI GDGCHC 2 cut(s) 318, 363
SfaNI GCATC 4 cut(s) 306, 317, 442, 699
SfcI CTRYAG 1 cut(s) 410
SfoI GGCGCC 3 cut(s) 336, 573, 606
Sfr303I CCGCGG 1 cut(s) 378
SgrAI CRCCGGYG 1 cut(s) 574
SgrBI CCGCGG 1 cut(s) 378
SinI GGWCC 1 cut(s) 647
Sse9I AATT 1 cut(s) 667
SspDI GGCGCC 3 cut(s) 334, 571, 604
SstI GAGCTC 1 cut(s) 318
StyD4I CCNGG 4 cut(s) 303, 369, 486, 554
TaaI ACNGT 1 cut(s) 62
TaiI ACGT 4 cut(s) 144, 178, 537, 662
TaqI TCGA 1 cut(s) 150
TaqII GACCGA 1 cut(s) 550
TasI AATT 1 cut(s) 667
TatI WGTACW 1 cut(s) 180
TauI GCSGC 2 cut(s) 377, 572
TfiI GAWTC 1 cut(s) 506
Tru1I TTAA 1 cut(s) 114
Tru9I TTAA 1 cut(s) 114
TscAI CASTG 1 cut(s) 445
TseI GCWGC 3 cut(s) 75, 294, 409
TspDTI ATGAA 1 cut(s) 498
TspGWI ACGGA 3 cut(s) 55, 115, 639
TspRI CASTG 1 cut(s) 445
Tth111I GACNNNGTC 1 cut(s) 634
Van91I CCANNNNNTGG 1 cut(s) 614
VpaK11BI GGWCC 1 cut(s) 647
XapI RAATTY 1 cut(s) 667
ZraI GACGTC 1 cut(s) 142
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.