Prupe.7G089000_v2.0.a1

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0001599
Physical Location & Seq
Forward (+)
12262744 .. 12265739
2996 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G089000.1

Sequence Viewer

Length: 981 bp
ATGGGCTCCCTCCCTCACATAGTAGAAGACTGCATGGGCGTCCTCCAAGTCTTTAGCGATGGTACGATCAACCGTTGCTCTTTGATGGACATGGACTTCAACATTCCTGTCGTTGATGACAACTCTGTGGCCTTCAAAGACGTCACTTTCGACAAAAGACACAACCTCTCCCTCCGTTTGTACAAACCCAAATCGGCAAACCGCAATTCCAAGCTCCCCGTGGTGTTCTACTTTCATGGCGGCGGTTTTTGTGTCGGCTCATGTGTGTGGCCAAACTTCTACAACTGCTGCCTCCGCCTGTCCGCGGTGCTCCATGCTCTAGTGGTTGCCCCGGACTACAGGCTTGCGCCGGAGCACAAGCTCCCGGCGCCAATTGATGATGCGTTGAGTGCGGTGGAGTGGCTGCAAAGGGAGGCTTTGATGAGTAAAAGTGGCGATTGTGATGCATGGATGGGTGGTACCGCTGACTTTGACCGGGTTTTCATTGTGGGAGACTCTTCTGGCGGGAATATGGCCCACCACCTGGCGGTTCGGCTCGGGGCTGGGTCAGCCAAGGTGGCCCCGGTTCGGGTACGTGGGTATGTGCTACTAGCGCCATTTTTTGGTGGGGTGGAGAGGACAAGGTCAGAGGAGGGGCCTTGTGAGGCACTGTTGAGTTTGGACATACTCGACAGATTTTGGAGACTCTCAATGCCAGCAGGAGAAACCAGAGACCACCCACTGGTGAACCCATTTGGACCAAACAGCCTAAACCTAGAGAAAGTGGCCCTTGATCCGATCTTGGTTTTTGTGGGTGGCAATGAAATTTTGAAAGATAGGGTGGAGAGCTACTCGAGAAAGCTGAAAGAGTTGGGGAAGAAAATTGAGTATGTGGAACTTGAGGGACAACAACATGGGTTTTTAACGGCTGATCCATACTCAGAAGTCTCAAATGAAGCTTTGCAATTTATTAAAAGATTTATGATTGAAAACTCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

327

Amino Acids

36.15

Weight (kDa)

5.74

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 144
Acc65I GGTACC 1 cut(s) 458
AccB1I GGYRCC 2 cut(s) 367, 458
AccB7I CCANNNNNTGG 3 cut(s) 523, 602, 721
AccII CGCG 1 cut(s) 305
AclWI GGATC 2 cut(s) 767, 905
AcoI YGGCCR 1 cut(s) 269
AcsI RAATTY 1 cut(s) 804
AcyI GRCGYC 3 cut(s) 39, 141, 368
AfaI GTAC 4 cut(s) 64, 182, 460, 573
AfiI CCNNNNNNNGG 7 cut(s) 304, 523, 526, 567, 568, 602, 721
AgsI TTSAA 4 cut(s) 100, 136, 811, 968
AjnI CCWGG 1 cut(s) 522
AluBI AGCT 5 cut(s) 214, 361, 828, 841, 938
AluI AGCT 5 cut(s) 214, 361, 828, 841, 938
Alw21I GWGCWC 2 cut(s) 312, 357
Alw26I GTCTC 4 cut(s) 486, 676, 705, 931
AlwI GGATC 2 cut(s) 767, 905
Ama87I CYCGRG 2 cut(s) 536, 832
AoxI GGCC 6 cut(s) 129, 269, 513, 558, 635, 765
ApeKI GCWGC 2 cut(s) 288, 403
ApoI RAATTY 1 cut(s) 804
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
Asp718I GGTACC 1 cut(s) 458
AspLEI GCGC 3 cut(s) 349, 370, 595
AspS9I GGNCC 5 cut(s) 514, 559, 635, 737, 766
AsuC2I CCSGG 4 cut(s) 332, 365, 476, 563
AsuHPI GGTGA 1 cut(s) 736
AvaI CYCGRG 2 cut(s) 536, 832
AvaII GGWCC 1 cut(s) 737
BaeI ACNNNNGTAYC 2 cut(s) 563, 596
BalI TGGCCA 1 cut(s) 271
BanI GGYRCC 2 cut(s) 367, 458
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 2 cut(s) 312, 357
BbvI GCAGC 2 cut(s) 275, 390
BccI CCATC 3 cut(s) 53, 79, 445
BceAI ACGGC 1 cut(s) 921
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BciT130I CCWGG 1 cut(s) 524
BcnI CCSGG 4 cut(s) 332, 365, 476, 563
BcoDI GTCTC 4 cut(s) 486, 676, 705, 931
BfaI CTAG 3 cut(s) 320, 590, 755
BfmI CTRYAG 1 cut(s) 337
BfoI RGCGCY 2 cut(s) 371, 596
BglI GCCNNNNNGGC 1 cut(s) 557
BisI GCNGC 3 cut(s) 241, 289, 404
BlsI GCNGC 3 cut(s) 242, 290, 405
Bme1390I CCNGG 5 cut(s) 332, 365, 476, 524, 563
Bme18I GGWCC 1 cut(s) 737
BmeT110I CYCGRG 2 cut(s) 536, 832
BmgT120I GGNCC 5 cut(s) 514, 559, 635, 737, 766
BmiI GGNNCC 5 cut(s) 7, 369, 460, 561, 636
BmrFI CCNGG 5 cut(s) 332, 365, 476, 524, 563
BmsI GCATC 2 cut(s) 370, 433
BpiI GAAGAC 1 cut(s) 33
BplI GAGNNNNNCTC 2 cut(s) 815, 847
BpuEI CTTGAG 1 cut(s) 899
BpuMI CCSGG 4 cut(s) 332, 365, 476, 563
BsaAI YACGTR 1 cut(s) 575
BsaHI GRCGYC 3 cut(s) 39, 141, 368
BsaI GGTCTC 1 cut(s) 705
BsaJI CCNNGG 5 cut(s) 219, 303, 330, 552, 561
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 7 cut(s) 304, 523, 526, 567, 568, 602, 721
Bse1I ACTGG 1 cut(s) 726
Bse3DI GCAATG 1 cut(s) 805
BseBI CCWGG 1 cut(s) 524
BseDI CCNNGG 5 cut(s) 219, 303, 330, 552, 561
BseGI GGATG 1 cut(s) 456
BseLI CCNNNNNNNGG 7 cut(s) 304, 523, 526, 567, 568, 602, 721
BseMI GCAATG 1 cut(s) 805
BseMII CTCAG 1 cut(s) 933
BseNI ACTGG 1 cut(s) 726
BseRI GAGGAG 1 cut(s) 644
BseXI GCAGC 2 cut(s) 275, 390
BseYI CCCAGC 1 cut(s) 542
Bsh1236I CGCG 1 cut(s) 305
BshFI GGCC 6 cut(s) 131, 271, 515, 560, 637, 767
BshNI GGYRCC 2 cut(s) 367, 458
BsiHKAI GWGCWC 2 cut(s) 312, 357
BsiHKCI CYCGRG 2 cut(s) 536, 832
BsiSI CCGG 5 cut(s) 332, 350, 365, 475, 563
BslFI GGGAC 1 cut(s) 897
BslI CCNNNNNNNGG 7 cut(s) 304, 523, 526, 567, 568, 602, 721
BsmAI GTCTC 4 cut(s) 486, 676, 705, 931
BsmFI GGGAC 1 cut(s) 897
BsnI GGCC 6 cut(s) 131, 271, 515, 560, 637, 767
Bso31I GGTCTC 1 cut(s) 705
BsoBI CYCGRG 2 cut(s) 536, 832
Bsp1286I GDGCHC 3 cut(s) 8, 312, 357
Bsp1407I TGTACA 1 cut(s) 180
Bsp143I GATC 4 cut(s) 66, 772, 777, 910
BspANI GGCC 6 cut(s) 131, 271, 515, 560, 637, 767
BspCNI CTCAG 1 cut(s) 932
BspFNI CGCG 1 cut(s) 305
BspLI GGNNCC 5 cut(s) 7, 369, 460, 561, 636
BspPI GGATC 2 cut(s) 767, 905
BspT107I GGYRCC 2 cut(s) 367, 458
BspTNI GGTCTC 1 cut(s) 705
BsrDI GCAATG 1 cut(s) 805
BsrGI TGTACA 1 cut(s) 180
BsrI ACTGG 1 cut(s) 726
BssECI CCNNGG 5 cut(s) 219, 303, 330, 552, 561
BssMI GATC 4 cut(s) 66, 772, 777, 910
BssNI GRCGYC 3 cut(s) 39, 141, 368
BssT1I CCWWGG 1 cut(s) 552
Bst2UI CCWGG 1 cut(s) 524
Bst4CI ACNGT 2 cut(s) 74, 651
Bst6I CTCTTC 1 cut(s) 502
BstACI GRCGYC 3 cut(s) 39, 141, 368
BstAUI TGTACA 1 cut(s) 180
BstBAI YACGTR 1 cut(s) 575
BstC8I GCNNGC 2 cut(s) 345, 696
BstDEI CTNAG 1 cut(s) 919
BstDSI CCRYGG 2 cut(s) 219, 303
BstF5I GGATG 1 cut(s) 456
BstFNI CGCG 1 cut(s) 305
BstH2I RGCGCY 2 cut(s) 371, 596
BstHHI GCGC 3 cut(s) 349, 370, 595
BstKTI GATC 4 cut(s) 69, 775, 780, 913
BstMAI GTCTC 4 cut(s) 486, 676, 705, 931
BstMBI GATC 4 cut(s) 66, 772, 777, 910
BstMWI GCNNNNNNNGC 6 cut(s) 294, 367, 389, 548, 557, 592
BstNI CCWGG 1 cut(s) 524
BstSCI CCNGG 5 cut(s) 330, 363, 474, 522, 561
BstSFI CTRYAG 1 cut(s) 337
BstUI CGCG 1 cut(s) 305
BstV1I GCAGC 2 cut(s) 275, 390
BstV2I GAAGAC 1 cut(s) 33
BsuRI GGCC 6 cut(s) 131, 271, 515, 560, 637, 767
BtgI CCRYGG 2 cut(s) 219, 303
BtgZI GCGATG 1 cut(s) 72
BtsCI GGATG 1 cut(s) 456
BtsIMutI CAGTG 2 cut(s) 647, 719
Cac8I GCNNGC 2 cut(s) 345, 696
CfoI GCGC 3 cut(s) 349, 370, 595
Cfr13I GGNCC 5 cut(s) 514, 559, 635, 737, 766
Cfr42I CCGCGG 1 cut(s) 306
CseI GACGC 1 cut(s) 28
Csp6I GTAC 4 cut(s) 63, 181, 459, 572
CviAII CATG 7 cut(s) 34, 91, 236, 261, 314, 447, 893
CviQI GTAC 4 cut(s) 63, 181, 459, 572
DdeI CTNAG 1 cut(s) 919
DinI GGCGCC 1 cut(s) 369
DpnI GATC 4 cut(s) 68, 774, 779, 912
DpnII GATC 4 cut(s) 66, 772, 777, 910
EaeI YGGCCR 1 cut(s) 269
Eam1104I CTCTTC 1 cut(s) 502
EarI CTCTTC 1 cut(s) 502
EciI GGCGGA 1 cut(s) 284
Eco130I CCWWGG 1 cut(s) 552
Eco24I GRGCYC 1 cut(s) 8
Eco31I GGTCTC 1 cut(s) 705
Eco47I GGWCC 1 cut(s) 737
Eco88I CYCGRG 2 cut(s) 536, 832
EcoO109I RGGNCCY 1 cut(s) 635
EcoRII CCWGG 1 cut(s) 522
EcoT14I CCWWGG 1 cut(s) 552
EcoT22I ATGCAT 1 cut(s) 448
EcoT38I GRGCYC 1 cut(s) 8
EgeI GGCGCC 1 cut(s) 369
EheI GGCGCC 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 552
FaeI CATG 7 cut(s) 37, 94, 239, 264, 317, 450, 896
FalI AAGNNNNNCTT 4 cut(s) 400, 432, 753, 785
FaqI GGGAC 1 cut(s) 897
FatI CATG 7 cut(s) 33, 90, 235, 260, 313, 446, 892
FauI CCCGC 1 cut(s) 497
Fnu4HI GCNGC 3 cut(s) 241, 289, 404
FokI GGATG 1 cut(s) 463
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 3 cut(s) 241, 289, 404
FspBI CTAG 3 cut(s) 320, 590, 755
GlaI GCGC 3 cut(s) 348, 369, 594
GluI GCNGC 3 cut(s) 241, 289, 404
GsaI CCCAGC 1 cut(s) 546
HaeII RGCGCY 2 cut(s) 371, 596
HaeIII GGCC 6 cut(s) 131, 271, 515, 560, 637, 767
HapII CCGG 5 cut(s) 332, 350, 365, 475, 563
HgaI GACGC 1 cut(s) 28
HhaI GCGC 3 cut(s) 349, 370, 595
Hin1I GRCGYC 3 cut(s) 39, 141, 368
Hin1II CATG 7 cut(s) 37, 94, 239, 264, 317, 450, 896
Hin6I GCGC 3 cut(s) 347, 368, 593
HinP1I GCGC 3 cut(s) 347, 368, 593
HindIII AAGCTT 1 cut(s) 936
HinfI GANTC 2 cut(s) 494, 684
HpaII CCGG 5 cut(s) 332, 350, 365, 475, 563
HphI GGTGA 1 cut(s) 736
Hpy166II GTNNAC 1 cut(s) 727
Hpy188I TCNGA 3 cut(s) 628, 777, 922
Hpy188III TCNNGA 1 cut(s) 834
Hpy8I GTNNAC 1 cut(s) 727
HpyAV CCTTC 1 cut(s) 142
HpyCH4III ACNGT 2 cut(s) 74, 651
HpyCH4IV ACGT 2 cut(s) 141, 574
HpyCH4V TGCA 4 cut(s) 33, 406, 446, 943
HpyF10VI GCNNNNNNNGC 6 cut(s) 294, 367, 389, 548, 557, 592
HpyF3I CTNAG 1 cut(s) 919
HpySE526I ACGT 2 cut(s) 141, 574
Hsp92I GRCGYC 3 cut(s) 39, 141, 368
Hsp92II CATG 7 cut(s) 37, 94, 239, 264, 317, 450, 896
HspAI GCGC 3 cut(s) 347, 368, 593
KasI GGCGCC 1 cut(s) 367
KpnI GGTACC 1 cut(s) 462
KspI CCGCGG 1 cut(s) 306
Kzo9I GATC 4 cut(s) 66, 772, 777, 910
LmnI GCTCC 5 cut(s) 11, 219, 315, 352, 366
Lsp1109I GCAGC 2 cut(s) 275, 390
LweI GCATC 2 cut(s) 370, 433
MaeI CTAG 3 cut(s) 320, 590, 755
MaeII ACGT 2 cut(s) 141, 574
MaeIII GTNAC 1 cut(s) 142
MalI GATC 4 cut(s) 68, 774, 779, 912
MboI GATC 4 cut(s) 66, 772, 777, 910
MboII GAAGA 3 cut(s) 38, 489, 868
MfeI CAATTG 1 cut(s) 372
MhlI GDGCHC 3 cut(s) 8, 312, 357
MlsI TGGCCA 1 cut(s) 271
MluCI AATT 5 cut(s) 205, 372, 804, 861, 944
MluNI TGGCCA 1 cut(s) 271
Mly113I GGCGCC 1 cut(s) 368
MlyI GAGTC 2 cut(s) 488, 678
Mox20I TGGCCA 1 cut(s) 271
Mph1103I ATGCAT 1 cut(s) 448
MscI TGGCCA 1 cut(s) 271
MseI TTAA 2 cut(s) 902, 951
MslI CAYNNNNRTG 1 cut(s) 265
Msp20I TGGCCA 1 cut(s) 271
MspA1I CMGCKG 2 cut(s) 305, 464
MspI CCGG 5 cut(s) 332, 350, 365, 475, 563
MspR9I CCNGG 5 cut(s) 332, 365, 476, 524, 563
MunI CAATTG 1 cut(s) 372
MvaI CCWGG 1 cut(s) 524
MvnI CGCG 1 cut(s) 305
MwoI GCNNNNNNNGC 6 cut(s) 294, 367, 389, 548, 557, 592
NarI GGCGCC 1 cut(s) 368
NciI CCSGG 4 cut(s) 332, 365, 476, 563
NdeII GATC 4 cut(s) 66, 772, 777, 910
NlaIII CATG 7 cut(s) 37, 94, 239, 264, 317, 450, 896
NlaIV GGNNCC 5 cut(s) 7, 369, 460, 561, 636
NmuCI GTSAC 1 cut(s) 142
NsiI ATGCAT 1 cut(s) 448
PaeR7I CTCGAG 1 cut(s) 832
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PflFI GACNNNGTC 1 cut(s) 622
PflMI CCANNNNNTGG 3 cut(s) 523, 602, 721
PkrI GCNGC 3 cut(s) 242, 290, 405
PleI GAGTC 2 cut(s) 488, 678
PluTI GGCGCC 1 cut(s) 371
PpsI GAGTC 2 cut(s) 488, 678
Ppu21I YACGTR 1 cut(s) 575
Psp6I CCWGG 1 cut(s) 522
PspFI CCCAGC 1 cut(s) 542
PspGI CCWGG 1 cut(s) 522
PspN4I GGNNCC 5 cut(s) 7, 369, 460, 561, 636
PspPI GGNCC 5 cut(s) 514, 559, 635, 737, 766
PsyI GACNNNGTC 1 cut(s) 622
RsaI GTAC 4 cut(s) 64, 182, 460, 573
RsaNI GTAC 4 cut(s) 63, 181, 459, 572
RseI CAYNNNNRTG 1 cut(s) 265
SacII CCGCGG 1 cut(s) 306
SaqAI TTAA 2 cut(s) 902, 951
SatI GCNGC 3 cut(s) 241, 289, 404
Sau3AI GATC 4 cut(s) 66, 772, 777, 910
Sau96I GGNCC 5 cut(s) 514, 559, 635, 737, 766
SchI GAGTC 2 cut(s) 488, 678
ScrFI CCNGG 5 cut(s) 332, 365, 476, 524, 563
SduI GDGCHC 3 cut(s) 8, 312, 357
SfaNI GCATC 2 cut(s) 370, 433
SfcI CTRYAG 1 cut(s) 337
SfoI GGCGCC 1 cut(s) 369
Sfr274I CTCGAG 1 cut(s) 832
Sfr303I CCGCGG 1 cut(s) 306
SgrBI CCGCGG 1 cut(s) 306
SinI GGWCC 1 cut(s) 737
SlaI CTCGAG 1 cut(s) 832
SmiMI CAYNNNNRTG 1 cut(s) 265
SmlI CTYRAG 2 cut(s) 832, 878
SmoI CTYRAG 2 cut(s) 832, 878
Sse9I AATT 5 cut(s) 205, 372, 804, 861, 944
SspDI GGCGCC 1 cut(s) 367
SspMI CTAG 3 cut(s) 320, 590, 755
StyD4I CCNGG 5 cut(s) 330, 363, 474, 522, 561
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 2 cut(s) 74, 651
TaiI ACGT 2 cut(s) 144, 577
TaqI TCGA 3 cut(s) 150, 669, 833
TasI AATT 5 cut(s) 205, 372, 804, 861, 944
TatI WGTACW 1 cut(s) 180
TauI GCSGC 1 cut(s) 243
Tru1I TTAA 2 cut(s) 902, 951
Tru9I TTAA 2 cut(s) 902, 951
TscAI CASTG 2 cut(s) 654, 726
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 2 cut(s) 288, 403
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 4 cut(s) 224, 472, 816, 948
TspGWI ACGGA 1 cut(s) 164
TspRI CASTG 2 cut(s) 654, 726
Tth111I GACNNNGTC 1 cut(s) 622
Van91I CCANNNNNTGG 3 cut(s) 523, 602, 721
VpaK11BI GGWCC 1 cut(s) 737
XapI RAATTY 1 cut(s) 804
XcmI CCANNNNNNNNNTGG 1 cut(s) 217
XhoI CTCGAG 1 cut(s) 832
XspI CTAG 3 cut(s) 320, 590, 755
ZraI GACGTC 1 cut(s) 142
Zsp2I ATGCAT 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.