FvH4_6g21100

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001599
Physical Location & Seq
Reverse (-)
14684540 .. 14688164
3625 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g21100.t2

Sequence Viewer

Length: 999 bp
ATGGGTTCCCTCCCTCACATTGTCGAAGACTGCATGGGCGTTCTCCAGCTCTTTAGCGACGGATCCGTCCATCGCTGCTCTATGAAAGACATAAACTTCCCGTTTCCAGTAGTTCACGACGACTCTGTGGACTTCAAAGACGTCGTTTTCGACAAAAAGCTCAACATTTCCCTACGTCTGTACAGACCTAAAAGCCACGCGTCGGCCACACCCACCAACCTCCTCCCCGTGGTGTTTTACTTCCACGGGGGCGGTTTCTGTGTCGGCTCGCGGGTGTGGCCCAACTGTCACAACTGCTGCATCCGCCTCGCGTCCGGGCTTGACGCGCTCGTCGTGGCGCCGGACTACCGGCTCGCCCCCGAGCACAAGCTCCCGGCCGCGTTGGATGACGCGGTGAGTGCGGTGGAGTGGCTGCAGAGGGAGGCTTTGATGAGTGCGGGTAGTAGTGGTGACTGTGATGCATGGCTGGGTGGTGGTTTGGTAGACTTTGATCGGGTTTATGTGGTGGGTGACTCTTCAGGTGGTAATATGGCTCACCATGTGGCGGTGCGGCTCGGCTCCGGGTCAGTCAAGGCGGCGCCGGTACGGGTGCGGGGGTATGTTCTTTTGGCACCATTTTTTGGTGGAGAGGAGAGGACGAAGTCGGAGGAGGGTCCGCCGGAGACATTTTTGAATTTGGAGATACTTGACAGATTTTGGAGGCTGTCAATGCCAAAAGGAGAATCGAGGGATCATCCGATGGTGAACCCATTTGGACCAATCAGCCTCAGCCTTAAGGAGGTGGATCTTGATCCCATCTTGGTTATGGTGGGTGGAAATGAAATGCTGAAAGATAGGGTGGAAATATACTCGAGGAGGCTGAAAGAGTTGGGAAAGGAGATTGAGTACGTGGAATTTGATGGACAACAACATGGCTTTCTCACAAATGATCCATACTCAGAAGTTGCAAATGAAGCCCTTCAAGTTATTAAGAGATTTATGATTGAAAACTCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

333

Amino Acids

36.72

Weight (kDa)

5.39

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 58 - 120 5.2e-06 Carboxylesterase family
BD-FAE PF20434 72 - 142 3.4e-07 BD-FAE
Abhydrolase_3 PF07859 78 - 226 1.9e-37 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 65, 329
AatII GACGTC 1 cut(s) 144
AccB1I GGYRCC 3 cut(s) 337, 577, 610
AccB7I CCANNNNNTGG 1 cut(s) 620
AccI GTMKAC 1 cut(s) 483
AccII CGCG 6 cut(s) 200, 271, 311, 326, 380, 392
AclWI GGATC 6 cut(s) 57, 70, 738, 785, 792, 923
AcoI YGGCCR 2 cut(s) 204, 375
AcsI RAATTY 2 cut(s) 673, 893
AcuI CTGAAG 1 cut(s) 501
AcyI GRCGYC 3 cut(s) 141, 338, 578
AdeI CACNNNGTG 1 cut(s) 541
AfaI GTAC 3 cut(s) 182, 585, 887
AfiI CCNNNNNNNGG 5 cut(s) 202, 229, 544, 620, 778
AflII CTTAAG 1 cut(s) 773
AflIII ACRYGT 1 cut(s) 198
AgsI TTSAA 4 cut(s) 136, 673, 962, 986
AluBI AGCT 3 cut(s) 49, 160, 370
AluI AGCT 3 cut(s) 49, 160, 370
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 1 cut(s) 656
AlwI GGATC 6 cut(s) 57, 70, 738, 785, 792, 923
Ama87I CYCGRG 2 cut(s) 359, 850
AoxI GGCC 3 cut(s) 204, 278, 375
ApeKI GCWGC 3 cut(s) 75, 297, 412
ApoI RAATTY 2 cut(s) 673, 893
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
Asp700I GAANNNNTTC 1 cut(s) 957
AspLEI GCGC 3 cut(s) 328, 340, 580
AspS9I GGNCC 3 cut(s) 279, 653, 755
AsuC2I CCSGG 3 cut(s) 316, 374, 562
AsuHPI GGTGA 5 cut(s) 406, 461, 521, 527, 754
AvaI CYCGRG 2 cut(s) 359, 850
AvaII GGWCC 2 cut(s) 653, 755
BamHI GGATCC 1 cut(s) 62
BanI GGYRCC 3 cut(s) 337, 577, 610
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 1 cut(s) 366
BbvCI CCTCAGC 1 cut(s) 767
BbvI GCAGC 3 cut(s) 62, 284, 399
BccI CCATC 4 cut(s) 78, 733, 803, 893
BcgI CGANNNNNNTGC 2 cut(s) 289, 323
BcnI CCSGG 3 cut(s) 316, 374, 562
BcoDI GTCTC 1 cut(s) 656
BfmI CTRYAG 1 cut(s) 413
BfoI RGCGCY 2 cut(s) 341, 581
BfrI CTTAAG 1 cut(s) 773
BisI GCNGC 6 cut(s) 76, 298, 378, 413, 551, 576
BlsI GCNGC 6 cut(s) 77, 299, 379, 414, 552, 577
Bme1390I CCNGG 3 cut(s) 316, 374, 562
Bme18I GGWCC 2 cut(s) 653, 755
BmeT110I CYCGRG 2 cut(s) 359, 850
BmgT120I GGNCC 3 cut(s) 279, 653, 755
BmiI GGNNCC 7 cut(s) 7, 64, 339, 559, 579, 612, 654
BmrFI CCNGG 3 cut(s) 316, 374, 562
BmsI GCATC 2 cut(s) 309, 448
BpiI GAAGAC 1 cut(s) 33
BpmI CTGGAG 1 cut(s) 29
Bpu10I CCTNAGC 1 cut(s) 767
BpuMI CCSGG 3 cut(s) 316, 374, 562
BsaAI YACGTR 1 cut(s) 889
BsaBI GATNNNNATC 1 cut(s) 789
BsaHI GRCGYC 3 cut(s) 141, 338, 578
BsaJI CCNNGG 2 cut(s) 228, 244
BsaXI ACNNNNNCTCC 2 cut(s) 869, 899
Bsc4I CCNNNNNNNGG 5 cut(s) 202, 229, 544, 620, 778
Bse118I RCCGGY 2 cut(s) 348, 580
Bse1I ACTGG 1 cut(s) 107
Bse8I GATNNNNATC 1 cut(s) 789
BseDI CCNNGG 2 cut(s) 228, 244
BseGI GGATG 3 cut(s) 300, 391, 733
BseJI GATNNNNATC 1 cut(s) 789
BseLI CCNNNNNNNGG 5 cut(s) 202, 229, 544, 620, 778
BseMII CTCAG 2 cut(s) 781, 951
BseNI ACTGG 1 cut(s) 107
BseRI GAGGAG 4 cut(s) 212, 644, 662, 868
BseX3I CGGCCG 1 cut(s) 375
BseXI GCAGC 3 cut(s) 62, 284, 399
BseYI CCCAGC 1 cut(s) 466
Bsh1236I CGCG 6 cut(s) 200, 271, 311, 326, 380, 392
Bsh1285I CGRYCG 1 cut(s) 378
BshFI GGCC 3 cut(s) 206, 280, 377
BshNI GGYRCC 3 cut(s) 337, 577, 610
BsiEI CGRYCG 1 cut(s) 378
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 2 cut(s) 359, 850
BsiSI CCGG 7 cut(s) 315, 341, 349, 374, 561, 581, 659
BslI CCNNNNNNNGG 5 cut(s) 202, 229, 544, 620, 778
BsmAI GTCTC 1 cut(s) 656
BsnI GGCC 3 cut(s) 206, 280, 377
BsoBI CYCGRG 2 cut(s) 359, 850
Bsp1286I GDGCHC 1 cut(s) 366
Bsp1407I TGTACA 1 cut(s) 180
Bsp143I GATC 6 cut(s) 62, 490, 730, 784, 790, 928
BspANI GGCC 3 cut(s) 206, 280, 377
BspCNI CTCAG 2 cut(s) 780, 950
BspFNI CGCG 6 cut(s) 200, 271, 311, 326, 380, 392
BspLI GGNNCC 7 cut(s) 7, 64, 339, 559, 579, 612, 654
BspMAI CTGCAG 1 cut(s) 417
BspPI GGATC 6 cut(s) 57, 70, 738, 785, 792, 923
BspT107I GGYRCC 3 cut(s) 337, 577, 610
BspTI CTTAAG 1 cut(s) 773
BsrFI RCCGGY 2 cut(s) 348, 580
BsrGI TGTACA 1 cut(s) 180
BsrI ACTGG 1 cut(s) 107
BssAI RCCGGY 2 cut(s) 348, 580
BssECI CCNNGG 2 cut(s) 228, 244
BssMI GATC 6 cut(s) 62, 490, 730, 784, 790, 928
BssNI GRCGYC 3 cut(s) 141, 338, 578
Bst4CI ACNGT 2 cut(s) 287, 455
Bst6I CTCTTC 1 cut(s) 520
BstACI GRCGYC 3 cut(s) 141, 338, 578
BstAFI CTTAAG 1 cut(s) 773
BstAUI TGTACA 1 cut(s) 180
BstBAI YACGTR 1 cut(s) 889
BstC8I GCNNGC 2 cut(s) 269, 354
BstDEI CTNAG 2 cut(s) 767, 937
BstDSI CCRYGG 2 cut(s) 228, 244
BstENI CCTNNNNNAGG 1 cut(s) 776
BstF5I GGATG 3 cut(s) 300, 391, 733
BstFNI CGCG 6 cut(s) 200, 271, 311, 326, 380, 392
BstH2I RGCGCY 2 cut(s) 341, 581
BstHHI GCGC 3 cut(s) 328, 340, 580
BstKTI GATC 6 cut(s) 65, 493, 733, 787, 793, 931
BstMAI GTCTC 1 cut(s) 656
BstMBI GATC 6 cut(s) 62, 490, 730, 784, 790, 928
BstMCI CGRYCG 1 cut(s) 378
BstMWI GCNNNNNNNGC 6 cut(s) 277, 303, 325, 398, 709, 953
BstSCI CCNGG 3 cut(s) 314, 372, 560
BstSFI CTRYAG 1 cut(s) 413
BstUI CGCG 6 cut(s) 200, 271, 311, 326, 380, 392
BstV1I GCAGC 3 cut(s) 62, 284, 399
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 2 cut(s) 62, 784
BstYI RGATCY 2 cut(s) 62, 784
BstZI CGGCCG 1 cut(s) 375
BsuRI GGCC 3 cut(s) 206, 280, 377
BtgI CCRYGG 2 cut(s) 228, 244
BtgZI GCGATG 1 cut(s) 56
BtsCI GGATG 3 cut(s) 300, 391, 733
Cac8I GCNNGC 2 cut(s) 269, 354
CfoI GCGC 3 cut(s) 328, 340, 580
Cfr10I RCCGGY 2 cut(s) 348, 580
Cfr13I GGNCC 3 cut(s) 279, 653, 755
CseI GACGC 4 cut(s) 189, 300, 332, 398
Csp6I GTAC 3 cut(s) 181, 584, 886
CviAII CATG 4 cut(s) 34, 462, 539, 911
CviQI GTAC 3 cut(s) 181, 584, 886
DdeI CTNAG 2 cut(s) 767, 937
DinI GGCGCC 2 cut(s) 339, 579
DpnI GATC 6 cut(s) 64, 492, 732, 786, 792, 930
DpnII GATC 6 cut(s) 62, 490, 730, 784, 790, 928
DraIII CACNNNGTG 1 cut(s) 541
DrdI GACNNNNNNGTC 2 cut(s) 65, 329
DseDI GACNNNNNNGTC 2 cut(s) 65, 329
EaeI YGGCCR 2 cut(s) 204, 375
EagI CGGCCG 1 cut(s) 375
Eam1104I CTCTTC 1 cut(s) 520
EarI CTCTTC 1 cut(s) 520
EciI GGCGGA 2 cut(s) 293, 645
EclXI CGGCCG 1 cut(s) 375
Eco47I GGWCC 2 cut(s) 653, 755
Eco52I CGGCCG 1 cut(s) 375
Eco57I CTGAAG 1 cut(s) 501
Eco88I CYCGRG 2 cut(s) 359, 850
EcoNI CCTNNNNNAGG 1 cut(s) 776
EcoT22I ATGCAT 1 cut(s) 463
EgeI GGCGCC 2 cut(s) 339, 579
EheI GGCGCC 2 cut(s) 339, 579
FaeI CATG 4 cut(s) 37, 465, 542, 914
FatI CATG 4 cut(s) 33, 461, 538, 910
FauI CCCGC 3 cut(s) 264, 430, 585
FblI GTMKAC 1 cut(s) 483
Fnu4HI GCNGC 6 cut(s) 76, 298, 378, 413, 551, 576
FokI GGATG 3 cut(s) 287, 398, 720
Fsp4HI GCNGC 6 cut(s) 76, 298, 378, 413, 551, 576
GlaI GCGC 3 cut(s) 327, 339, 579
GluI GCNGC 6 cut(s) 76, 298, 378, 413, 551, 576
GsaI CCCAGC 1 cut(s) 470
GsuI CTGGAG 1 cut(s) 29
HaeII RGCGCY 2 cut(s) 341, 581
HaeIII GGCC 3 cut(s) 206, 280, 377
HapII CCGG 7 cut(s) 315, 341, 349, 374, 561, 581, 659
HgaI GACGC 4 cut(s) 189, 300, 332, 398
HhaI GCGC 3 cut(s) 328, 340, 580
Hin1I GRCGYC 3 cut(s) 141, 338, 578
Hin1II CATG 4 cut(s) 37, 465, 542, 914
Hin6I GCGC 3 cut(s) 326, 338, 578
HinP1I GCGC 3 cut(s) 326, 338, 578
HinfI GANTC 3 cut(s) 122, 512, 722
HpaII CCGG 7 cut(s) 315, 341, 349, 374, 561, 581, 659
HphI GGTGA 5 cut(s) 406, 461, 521, 527, 754
Hpy166II GTNNAC 4 cut(s) 115, 130, 484, 745
Hpy188I TCNGA 3 cut(s) 646, 738, 940
Hpy188III TCNNGA 2 cut(s) 116, 788
Hpy8I GTNNAC 4 cut(s) 115, 130, 484, 745
Hpy99I CGWCG 5 cut(s) 62, 122, 146, 205, 335
HpyAV CCTTC 1 cut(s) 968
HpyCH4III ACNGT 2 cut(s) 287, 455
HpyCH4IV ACGT 3 cut(s) 141, 175, 888
HpyCH4V TGCA 5 cut(s) 33, 300, 415, 461, 947
HpyF10VI GCNNNNNNNGC 6 cut(s) 277, 303, 325, 398, 709, 953
HpyF3I CTNAG 2 cut(s) 767, 937
HpySE526I ACGT 3 cut(s) 141, 175, 888
Hsp92I GRCGYC 3 cut(s) 141, 338, 578
Hsp92II CATG 4 cut(s) 37, 465, 542, 914
HspAI GCGC 3 cut(s) 326, 338, 578
KasI GGCGCC 2 cut(s) 337, 577
Kzo9I GATC 6 cut(s) 62, 490, 730, 784, 790, 928
LmnI GCTCC 2 cut(s) 375, 563
Lsp1109I GCAGC 3 cut(s) 62, 284, 399
LweI GCATC 2 cut(s) 309, 448
MaeII ACGT 3 cut(s) 141, 175, 888
MaeIII GTNAC 3 cut(s) 287, 449, 509
MalI GATC 6 cut(s) 64, 492, 732, 786, 792, 930
MboI GATC 6 cut(s) 62, 490, 730, 784, 790, 928
MboII GAAGA 2 cut(s) 38, 507
MflI RGATCY 2 cut(s) 62, 784
MhlI GDGCHC 1 cut(s) 366
MluCI AATT 2 cut(s) 673, 893
MluI ACGCGT 1 cut(s) 198
Mly113I GGCGCC 2 cut(s) 338, 578
MlyI GAGTC 2 cut(s) 116, 506
MmeI TCCRAC 2 cut(s) 363, 624
Mph1103I ATGCAT 1 cut(s) 463
MroXI GAANNNNTTC 1 cut(s) 957
MseI TTAA 2 cut(s) 774, 969
MspCI CTTAAG 1 cut(s) 773
MspI CCGG 7 cut(s) 315, 341, 349, 374, 561, 581, 659
MspR9I CCNGG 3 cut(s) 316, 374, 562
MvnI CGCG 6 cut(s) 200, 271, 311, 326, 380, 392
MwoI GCNNNNNNNGC 6 cut(s) 277, 303, 325, 398, 709, 953
NarI GGCGCC 2 cut(s) 338, 578
NciI CCSGG 3 cut(s) 316, 374, 562
NdeII GATC 6 cut(s) 62, 490, 730, 784, 790, 928
NlaIII CATG 4 cut(s) 37, 465, 542, 914
NlaIV GGNNCC 7 cut(s) 7, 64, 339, 559, 579, 612, 654
NmeAIII GCCGAG 1 cut(s) 534
NmuCI GTSAC 3 cut(s) 287, 449, 509
NsiI ATGCAT 1 cut(s) 463
PaeR7I CTCGAG 1 cut(s) 850
PcsI WCGNNNNNNNCGW 2 cut(s) 147, 330
PdmI GAANNNNTTC 1 cut(s) 957
PfeI GAWTC 1 cut(s) 722
PflFI GACNNNGTC 1 cut(s) 640
PflMI CCANNNNNTGG 1 cut(s) 620
PkrI GCNGC 6 cut(s) 77, 299, 379, 414, 552, 577
PleI GAGTC 2 cut(s) 116, 506
PluTI GGCGCC 2 cut(s) 341, 581
PpsI GAGTC 2 cut(s) 116, 506
Ppu21I YACGTR 1 cut(s) 889
PspFI CCCAGC 1 cut(s) 466
PspN4I GGNNCC 7 cut(s) 7, 64, 339, 559, 579, 612, 654
PspPI GGNCC 3 cut(s) 279, 653, 755
PspXI VCTCGAGB 1 cut(s) 850
PstI CTGCAG 1 cut(s) 417
PsuI RGATCY 2 cut(s) 62, 784
PsyI GACNNNGTC 1 cut(s) 640
RsaI GTAC 3 cut(s) 182, 585, 887
RsaNI GTAC 3 cut(s) 181, 584, 886
SaqAI TTAA 2 cut(s) 774, 969
SatI GCNGC 6 cut(s) 76, 298, 378, 413, 551, 576
Sau3AI GATC 6 cut(s) 62, 490, 730, 784, 790, 928
Sau96I GGNCC 3 cut(s) 279, 653, 755
SchI GAGTC 2 cut(s) 116, 506
ScrFI CCNGG 3 cut(s) 316, 374, 562
SduI GDGCHC 1 cut(s) 366
SfaNI GCATC 2 cut(s) 309, 448
SfcI CTRYAG 1 cut(s) 413
SfoI GGCGCC 2 cut(s) 339, 579
Sfr274I CTCGAG 1 cut(s) 850
SinI GGWCC 2 cut(s) 653, 755
SlaI CTCGAG 1 cut(s) 850
SmlI CTYRAG 2 cut(s) 773, 850
SmoI CTYRAG 2 cut(s) 773, 850
Sse9I AATT 2 cut(s) 673, 893
SspDI GGCGCC 2 cut(s) 337, 577
StyD4I CCNGG 3 cut(s) 314, 372, 560
TaaI ACNGT 2 cut(s) 287, 455
TaiI ACGT 3 cut(s) 144, 178, 891
TaqI TCGA 4 cut(s) 24, 150, 725, 851
TasI AATT 2 cut(s) 673, 893
TatI WGTACW 1 cut(s) 180
TauI GCSGC 3 cut(s) 380, 553, 578
TfiI GAWTC 1 cut(s) 722
Tru1I TTAA 2 cut(s) 774, 969
Tru9I TTAA 2 cut(s) 774, 969
TseFI GTSAC 3 cut(s) 287, 449, 509
TseI GCWGC 3 cut(s) 75, 297, 412
Tsp45I GTSAC 3 cut(s) 287, 449, 509
TspDTI ATGAA 3 cut(s) 98, 834, 966
TspGWI ACGGA 2 cut(s) 55, 75
Tth111I GACNNNGTC 1 cut(s) 640
Van91I CCANNNNNTGG 1 cut(s) 620
Vha464I CTTAAG 1 cut(s) 773
VpaK11BI GGWCC 2 cut(s) 653, 755
XagI CCTNNNNNAGG 1 cut(s) 776
XapI RAATTY 2 cut(s) 673, 893
XcmI CCANNNNNNNNNTGG 1 cut(s) 802
XhoI CTCGAG 1 cut(s) 850
XmiI GTMKAC 1 cut(s) 483
XmnI GAANNNNTTC 1 cut(s) 957
ZraI GACGTC 1 cut(s) 142
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.