Rroxscaffold_6G00404570

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001599
Physical Location & Seq
Forward (+)
27061511 .. 27064840
3330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00404570.1

Sequence Viewer

Length: 567 bp
ATGAGTGAGGGCAGTGGTGATTGTGATGCATGGCTGGGTGGTGGTATGGTTGACTTTGACCGGGTTTATGTGGTGGGCGACTCATCAGGAGGTAATATGGCTCACCACGTGGCCGTACGGCTTGGAGCGGGGTCGGTCAAGGCGGCGCCGGTGCGGGTACGCGGATATGTGCTTTTGGCGCCATTTTTCGGTGGAGTGGAGAGGACAAAGTCGGAGGAGGGTCCGTCGGAGACGTTTTTGAATTTGGAGATACTTGACAGATTTTGGAGGCTCTCATTGCCAAAAGGAGAAACCAGGGATCATCCAATGGCCAACCCATTTGGACCAAACAGCCCCAGCCTTGAGGAGGTGGATCTTGATCCCATCTTGGTTATTGTGGGTGGCAATGAAATGTTGAAAGATAGAGTGGAGATCTACTCGAGGAGACTGAAAGAGTTGGGAAAGAAGATTGAGTATGTGGATTTTCATGGACAACAACATGGCTTTCTCACAAATGATCCATACTCAGAAGTGGCAAATGAAGCACTGCAAGTTATTAAGAGATTTATGATTGAAAACTCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

188

Amino Acids

20.78

Weight (kDa)

5.14

Isoelectric Point (pI)

42.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 17 - 163 3.4e-30 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 145, 178
AccBSI CCGCTC 1 cut(s) 128
AccII CGCG 1 cut(s) 162
AciI CCGC 4 cut(s) 128, 143, 154, 162
AclWI GGATC 4 cut(s) 306, 353, 360, 491
AcoI YGGCCR 2 cut(s) 111, 309
AcsI RAATTY 1 cut(s) 241
AcvI CACGTG 1 cut(s) 109
AcyI GRCGYC 2 cut(s) 146, 179
AdeI CACNNNGTG 1 cut(s) 109
AfaI GTAC 2 cut(s) 117, 159
AfiI CCNNNNNNNGG 2 cut(s) 188, 346
AgsI TTSAA 3 cut(s) 241, 397, 554
AjnI CCWGG 1 cut(s) 293
Alw26I GTCTC 2 cut(s) 224, 418
AlwI GGATC 4 cut(s) 306, 353, 360, 491
Ama87I CYCGRG 1 cut(s) 418
AoxI GGCC 2 cut(s) 111, 309
ApoI RAATTY 1 cut(s) 241
AspLEI GCGC 2 cut(s) 148, 181
AspS9I GGNCC 2 cut(s) 221, 323
AsuC2I CCSGG 1 cut(s) 62
AsuHPI GGTGA 2 cut(s) 29, 95
AvaI CYCGRG 1 cut(s) 418
AvaII GGWCC 2 cut(s) 221, 323
BalI TGGCCA 1 cut(s) 311
BanI GGYRCC 2 cut(s) 145, 178
BbrPI CACGTG 1 cut(s) 109
BccI CCATC 1 cut(s) 371
BceAI ACGGC 2 cut(s) 98, 134
BciT130I CCWGG 1 cut(s) 295
BcnI CCSGG 1 cut(s) 62
BcoDI GTCTC 2 cut(s) 224, 418
BfoI RGCGCY 2 cut(s) 149, 182
BglII AGATCT 1 cut(s) 411
BisI GCNGC 1 cut(s) 144
BlsI GCNGC 1 cut(s) 145
Bme1390I CCNGG 2 cut(s) 62, 295
Bme18I GGWCC 2 cut(s) 221, 323
BmeT110I CYCGRG 1 cut(s) 418
BmgT120I GGNCC 2 cut(s) 221, 323
BmiI GGNNCC 3 cut(s) 147, 180, 222
BmrFI CCNGG 2 cut(s) 62, 295
BmsI GCATC 1 cut(s) 16
BplI GAGNNNNNCTC 2 cut(s) 401, 433
BpuEI CTTGAG 1 cut(s) 362
BpuMI CCSGG 1 cut(s) 62
BsaAI YACGTR 1 cut(s) 109
BsaBI GATNNNNATC 1 cut(s) 357
BsaHI GRCGYC 2 cut(s) 146, 179
BsaJI CCNNGG 1 cut(s) 294
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 346
Bse118I RCCGGY 1 cut(s) 148
Bse3DI GCAATG 2 cut(s) 275, 391
Bse8I GATNNNNATC 1 cut(s) 357
BseBI CCWGG 1 cut(s) 295
BseDI CCNNGG 1 cut(s) 294
BseGI GGATG 1 cut(s) 301
BseJI GATNNNNATC 1 cut(s) 357
BseLI CCNNNNNNNGG 2 cut(s) 188, 346
BseMI GCAATG 2 cut(s) 275, 391
BseMII CTCAG 1 cut(s) 519
BseRI GAGGAG 3 cut(s) 230, 359, 436
BseYI CCCAGC 2 cut(s) 34, 335
Bsh1236I CGCG 1 cut(s) 162
BshFI GGCC 2 cut(s) 113, 311
BshNI GGYRCC 2 cut(s) 145, 178
BsiHKCI CYCGRG 1 cut(s) 418
BsiSI CCGG 2 cut(s) 61, 149
BsiWI CGTACG 1 cut(s) 115
BslI CCNNNNNNNGG 2 cut(s) 188, 346
BsmAI GTCTC 2 cut(s) 224, 418
BsmBI CGTCTC 1 cut(s) 224
BsnI GGCC 2 cut(s) 113, 311
BsoBI CYCGRG 1 cut(s) 418
Bsp143I GATC 5 cut(s) 298, 352, 358, 411, 496
BspACI CCGC 4 cut(s) 128, 143, 154, 162
BspANI GGCC 2 cut(s) 113, 311
BspCNI CTCAG 1 cut(s) 518
BspFNI CGCG 1 cut(s) 162
BspLI GGNNCC 3 cut(s) 147, 180, 222
BspPI GGATC 4 cut(s) 306, 353, 360, 491
BspT107I GGYRCC 2 cut(s) 145, 178
BsrBI CCGCTC 1 cut(s) 128
BsrDI GCAATG 2 cut(s) 275, 391
BsrFI RCCGGY 1 cut(s) 148
BssAI RCCGGY 1 cut(s) 148
BssECI CCNNGG 1 cut(s) 294
BssMI GATC 5 cut(s) 298, 352, 358, 411, 496
BssNI GRCGYC 2 cut(s) 146, 179
Bst2UI CCWGG 1 cut(s) 295
BstACI GRCGYC 2 cut(s) 146, 179
BstBAI YACGTR 1 cut(s) 109
BstDEI CTNAG 1 cut(s) 505
BstENI CCTNNNNNAGG 1 cut(s) 344
BstF5I GGATG 1 cut(s) 301
BstFNI CGCG 1 cut(s) 162
BstH2I RGCGCY 2 cut(s) 149, 182
BstHHI GCGC 2 cut(s) 148, 181
BstKTI GATC 5 cut(s) 301, 355, 361, 414, 499
BstMAI GTCTC 2 cut(s) 224, 418
BstMBI GATC 5 cut(s) 298, 352, 358, 411, 496
BstMWI GCNNNNNNNGC 3 cut(s) 178, 277, 521
BstNI CCWGG 1 cut(s) 295
BstSCI CCNGG 2 cut(s) 60, 293
BstUI CGCG 1 cut(s) 162
BstX2I RGATCY 2 cut(s) 352, 411
BstYI RGATCY 2 cut(s) 352, 411
BsuRI GGCC 2 cut(s) 113, 311
BtsCI GGATG 1 cut(s) 301
BtsI GCAGTG 2 cut(s) 19, 524
BtsIMutI CAGTG 2 cut(s) 19, 524
CfoI GCGC 2 cut(s) 148, 181
Cfr10I RCCGGY 1 cut(s) 148
Cfr13I GGNCC 2 cut(s) 221, 323
Csp6I GTAC 2 cut(s) 116, 158
CviAII CATG 3 cut(s) 30, 467, 479
CviJI RGCY 9 cut(s) 34, 101, 113, 121, 271, 311, 333, 339, 483
CviKI_1 RGCY 9 cut(s) 34, 101, 113, 121, 271, 311, 333, 339, 483
CviQI GTAC 2 cut(s) 116, 158
DdeI CTNAG 1 cut(s) 505
DinI GGCGCC 2 cut(s) 147, 180
DpnI GATC 5 cut(s) 300, 354, 360, 413, 498
DpnII GATC 5 cut(s) 298, 352, 358, 411, 496
DraIII CACNNNGTG 1 cut(s) 109
EaeI YGGCCR 2 cut(s) 111, 309
Eco47I GGWCC 2 cut(s) 221, 323
Eco72I CACGTG 1 cut(s) 109
Eco88I CYCGRG 1 cut(s) 418
EcoNI CCTNNNNNAGG 1 cut(s) 344
EcoRII CCWGG 1 cut(s) 293
EcoT22I ATGCAT 1 cut(s) 31
EgeI GGCGCC 2 cut(s) 147, 180
EheI GGCGCC 2 cut(s) 147, 180
Esp3I CGTCTC 1 cut(s) 224
FaeI CATG 3 cut(s) 33, 470, 482
FatI CATG 3 cut(s) 29, 466, 478
FauI CCCGC 2 cut(s) 121, 147
Fnu4HI GCNGC 1 cut(s) 144
FokI GGATG 1 cut(s) 288
Fsp4HI GCNGC 1 cut(s) 144
GlaI GCGC 2 cut(s) 147, 180
GluI GCNGC 1 cut(s) 144
GsaI CCCAGC 2 cut(s) 38, 339
HaeII RGCGCY 2 cut(s) 149, 182
HaeIII GGCC 2 cut(s) 113, 311
HapII CCGG 2 cut(s) 61, 149
HhaI GCGC 2 cut(s) 148, 181
Hin1I GRCGYC 2 cut(s) 146, 179
Hin1II CATG 3 cut(s) 33, 470, 482
Hin6I GCGC 2 cut(s) 146, 179
HinP1I GCGC 2 cut(s) 146, 179
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HinfI GANTC 1 cut(s) 80
HpaII CCGG 2 cut(s) 61, 149
HphI GGTGA 2 cut(s) 29, 95
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 3 cut(s) 214, 229, 508
Hpy188III TCNNGA 2 cut(s) 87, 356
Hpy8I GTNNAC 1 cut(s) 52
Hpy99I CGWCG 1 cut(s) 229
HpyCH4IV ACGT 2 cut(s) 108, 233
HpyCH4V TGCA 2 cut(s) 29, 529
HpyF10VI GCNNNNNNNGC 3 cut(s) 178, 277, 521
HpyF3I CTNAG 1 cut(s) 505
HpySE526I ACGT 2 cut(s) 108, 233
Hsp92I GRCGYC 2 cut(s) 146, 179
Hsp92II CATG 3 cut(s) 33, 470, 482
HspAI GCGC 2 cut(s) 146, 179
KasI GGCGCC 2 cut(s) 145, 178
Kzo9I GATC 5 cut(s) 298, 352, 358, 411, 496
LmnI GCTCC 1 cut(s) 125
LpnPI CCDG 7 cut(s) 20, 72, 74, 162, 280, 307, 349
LweI GCATC 1 cut(s) 16
MaeII ACGT 2 cut(s) 108, 233
MalI GATC 5 cut(s) 300, 354, 360, 413, 498
MbiI CCGCTC 1 cut(s) 128
MboI GATC 5 cut(s) 298, 352, 358, 411, 496
MboII GAAGA 1 cut(s) 457
MflI RGATCY 2 cut(s) 352, 411
MlsI TGGCCA 1 cut(s) 311
MluCI AATT 1 cut(s) 241
MluNI TGGCCA 1 cut(s) 311
Mly113I GGCGCC 2 cut(s) 146, 179
MlyI GAGTC 1 cut(s) 74
MmeI TCCRAC 2 cut(s) 192, 207
MnlI CCTC 8 cut(s) 83, 195, 208, 211, 261, 337, 340, 414
Mox20I TGGCCA 1 cut(s) 311
Mph1103I ATGCAT 1 cut(s) 31
MscI TGGCCA 1 cut(s) 311
MseI TTAA 1 cut(s) 537
Msp20I TGGCCA 1 cut(s) 311
MspI CCGG 2 cut(s) 61, 149
MspR9I CCNGG 2 cut(s) 62, 295
MvaI CCWGG 1 cut(s) 295
MvnI CGCG 1 cut(s) 162
MwoI GCNNNNNNNGC 3 cut(s) 178, 277, 521
NarI GGCGCC 2 cut(s) 146, 179
NciI CCSGG 1 cut(s) 62
NdeII GATC 5 cut(s) 298, 352, 358, 411, 496
NlaIII CATG 3 cut(s) 33, 470, 482
NlaIV GGNNCC 3 cut(s) 147, 180, 222
NsiI ATGCAT 1 cut(s) 31
PaeR7I CTCGAG 1 cut(s) 418
Pfl23II CGTACG 1 cut(s) 115
PflFI GACNNNGTC 1 cut(s) 208
PkrI GCNGC 1 cut(s) 145
PleI GAGTC 1 cut(s) 74
PluTI GGCGCC 2 cut(s) 149, 182
PmaCI CACGTG 1 cut(s) 109
PmlI CACGTG 1 cut(s) 109
PpsI GAGTC 1 cut(s) 74
Ppu21I YACGTR 1 cut(s) 109
Psp6I CCWGG 1 cut(s) 293
PspCI CACGTG 1 cut(s) 109
PspFI CCCAGC 2 cut(s) 34, 335
PspGI CCWGG 1 cut(s) 293
PspLI CGTACG 1 cut(s) 115
PspN4I GGNNCC 3 cut(s) 147, 180, 222
PspPI GGNCC 2 cut(s) 221, 323
PspXI VCTCGAGB 1 cut(s) 418
PsuI RGATCY 2 cut(s) 352, 411
PsyI GACNNNGTC 1 cut(s) 208
RsaI GTAC 2 cut(s) 117, 159
RsaNI GTAC 2 cut(s) 116, 158
SaqAI TTAA 1 cut(s) 537
SatI GCNGC 1 cut(s) 144
Sau3AI GATC 5 cut(s) 298, 352, 358, 411, 496
Sau96I GGNCC 2 cut(s) 221, 323
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 2 cut(s) 62, 295
SetI ASST 4 cut(s) 94, 111, 236, 351
SfaNI GCATC 1 cut(s) 16
SfoI GGCGCC 2 cut(s) 147, 180
Sfr274I CTCGAG 1 cut(s) 418
SgrAI CRCCGGYG 1 cut(s) 148
SinI GGWCC 2 cut(s) 221, 323
SlaI CTCGAG 1 cut(s) 418
SmlI CTYRAG 2 cut(s) 341, 418
SmoI CTYRAG 2 cut(s) 341, 418
Sse9I AATT 1 cut(s) 241
SsiI CCGC 4 cut(s) 128, 143, 154, 162
SspDI GGCGCC 2 cut(s) 145, 178
StyD4I CCNGG 2 cut(s) 60, 293
TaiI ACGT 2 cut(s) 111, 236
TaqI TCGA 1 cut(s) 419
TaqII GACCGA 1 cut(s) 124
TasI AATT 1 cut(s) 241
TauI GCSGC 1 cut(s) 146
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TscAI CASTG 2 cut(s) 19, 531
TspDTI ATGAA 3 cut(s) 402, 455, 534
TspGWI ACGGA 1 cut(s) 213
TspRI CASTG 2 cut(s) 19, 531
Tth111I GACNNNGTC 1 cut(s) 208
VpaK11BI GGWCC 2 cut(s) 221, 323
XagI CCTNNNNNAGG 1 cut(s) 344
XapI RAATTY 1 cut(s) 241
XhoI CTCGAG 1 cut(s) 418
Zsp2I ATGCAT 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.