Prupe.6G149100_v2.0.a1

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001599
Physical Location & Seq
Reverse (-)
12636902 .. 12638694
1793 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G149100.1

Sequence Viewer

Length: 1164 bp
ATGGCCCACTCCACCATATTTCCATACAAAACAACAAAACAACATGGAAAAAATACCCAACAACTCCACTCCCCCACCTCCCTCCTCCTCCCTATTTCTTCCCCACTTCTCTTCCTTCCCTTTCTCACAATTCTCGCAAGTCCTCCTCATCACCACAACACAAGCATGTCCACCTCAACCACCACCCCTCCCAACACTTTATCGTACGAAGAATGCCGCGGCGTTCTTCGCGTATACAACGACGGCTCCATCGTCCGCTCTCCCAACCCGAGCTTCGACGTCCCCATCCTGGACGACGGCTCCGTCGCCTGGAAGGACGTCGTTTTCGACCCGACCAATCACCTCCAACTCCGCCTCTACAAGCCAGCAGCAACGACACGCTCTCCCTCGTCGTCCAAAAAGCTCCCCATCTTCTACTACATCCACGGTGGAGGCTTCTGCATCGGCTCGCGCGCTTGGCCCAACTGCCAGAACTACTGCTTCCAGCTCGCTTCCCAGCTCCAGTGTGTCGTTGTTTCGCCCGACTATCGCCTCGCTCCCGAGCACCGCCTCCCGGCAGCGATCCACGACGGCGCCGCTGCTCTGCAGTGGCTCCGGGCCCAAGCCGAGGCCGACGAGCCGGACACGTGGTTGACTGACGTGGCTGATTTTGAAAACGTGTTTGTTTCGGGTGACTCGGCGGGCGGGAACATAGCTCATAACTTGGCGGTTCGGTTTGGGCCCGGTTCGGCAGAGTTGGGGCCGGTTCGGGTTAGGGGGTATCTGCTTTTGGCACCGTTTTTCGCTGGAACAGTTTTGAGTAAGTCGGAGGCCGAGGGGCCCAAAGATGCCTTTCTCAATTGGGAGCTCATTGACAGGTTTTGGAGGCTATCGATACCAATTGGAGAGAATACAGATCACCCACTTGTGAACCCATTTGGGCCATTTAGCCGGAGCCTTGAACCGGTGGCTCTTGATCCGATCCTAGTGGTTGTGGGTGGCAGTGATCTGCTCAAAGACCGAGCTGAAGATTACGCAAAGAGGCTCAAGAATTGGGGAAAGAAGGTTGAGTACGTGGAATTTGAAGGGGAGCAGCATGGTTTCTTCACCGTTCATCCTAATTCCCAAGCCGCAAAAGACTTGATGCCAATTCTCAAACGATTCATTACTGAAAATTCCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

388

Amino Acids

42.8

Weight (kDa)

6.27

Isoelectric Point (pI)

47.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 302
AatII GACGTC 2 cut(s) 282, 321
AccB1I GGYRCC 2 cut(s) 572, 772
AccBSI CCGCTC 1 cut(s) 258
AccI GTMKAC 1 cut(s) 234
AccII CGCG 4 cut(s) 219, 231, 451, 453
AclWI GGATC 3 cut(s) 556, 950, 955
AcsI RAATTY 2 cut(s) 1058, 1153
AcuI CTGAAG 1 cut(s) 1026
AcvI CACGTG 1 cut(s) 627
AcyI GRCGYC 3 cut(s) 279, 318, 573
AfaI GTAC 2 cut(s) 206, 1052
AfiI CCNNNNNNNGG 5 cut(s) 289, 309, 553, 607, 943
AflIII ACRYGT 2 cut(s) 624, 657
AgeI ACCGGT 1 cut(s) 943
AgsI TTSAA 3 cut(s) 653, 941, 1064
AjiI CACGTC 1 cut(s) 640
AjnI CCWGG 2 cut(s) 288, 308
AjuI GAANNNNNNNTTGG 2 cut(s) 257, 289
AluBI AGCT 8 cut(s) 273, 403, 487, 499, 695, 847, 1004, 1161
AluI AGCT 8 cut(s) 273, 403, 487, 499, 695, 847, 1004, 1161
Alw21I GWGCWC 2 cut(s) 546, 849
AlwI GGATC 3 cut(s) 556, 950, 955
Ama87I CYCGRG 2 cut(s) 268, 539
AoxI GGCC 9 cut(s) 3, 458, 597, 609, 719, 740, 810, 818, 920
ApaI GGGCCC 3 cut(s) 601, 723, 822
ApeKI GCWGC 4 cut(s) 368, 557, 578, 1072
ApoI RAATTY 2 cut(s) 1058, 1153
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
AsiGI ACCGGT 1 cut(s) 943
AspLEI GCGC 3 cut(s) 453, 455, 575
AsuC2I CCSGG 3 cut(s) 554, 596, 723
AsuHPI GGTGA 5 cut(s) 143, 332, 683, 890, 1078
AvaI CYCGRG 2 cut(s) 268, 539
BaeGI GKGCMC 3 cut(s) 601, 723, 822
BanI GGYRCC 2 cut(s) 572, 772
BanII GRGCYC 4 cut(s) 601, 723, 822, 849
BarI GAAGNNNNNNTAC 2 cut(s) 1034, 1066
BbrPI CACGTG 1 cut(s) 627
Bbv12I GWGCWC 2 cut(s) 546, 849
BbvI GCAGC 4 cut(s) 380, 565, 569, 1084
BccI CCATC 3 cut(s) 257, 293, 416
BceAI ACGGC 3 cut(s) 259, 313, 586
BciT130I CCWGG 2 cut(s) 290, 310
BcnI CCSGG 3 cut(s) 554, 596, 723
BfaI CTAG 1 cut(s) 965
BfmI CTRYAG 1 cut(s) 584
BfoI RGCGCY 1 cut(s) 576
BisI GCNGC 8 cut(s) 217, 220, 369, 558, 576, 579, 1073, 1110
BlsI GCNGC 8 cut(s) 218, 221, 370, 559, 577, 580, 1074, 1111
Bme1390I CCNGG 5 cut(s) 290, 310, 554, 596, 723
BmeT110I CYCGRG 2 cut(s) 268, 539
BmgBI CACGTC 1 cut(s) 640
BmrFI CCNGG 5 cut(s) 290, 310, 554, 596, 723
BmsI GCATC 3 cut(s) 450, 817, 1113
BpmI CTGGAG 1 cut(s) 485
BpuEI CTTGAG 1 cut(s) 1010
BpuMI CCSGG 3 cut(s) 554, 596, 723
Bsa29I ATCGAT 1 cut(s) 872
BsaAI YACGTR 2 cut(s) 627, 1054
BsaHI GRCGYC 3 cut(s) 279, 318, 573
BsaJI CCNNGG 4 cut(s) 217, 424, 606, 813
BsaWI WCCGGW 1 cut(s) 943
BsaXI ACNNNNNCTCC 8 cut(s) 172, 202, 230, 260, 284, 314, 367, 397
Bsc4I CCNNNNNNNGG 5 cut(s) 289, 309, 553, 607, 943
Bse118I RCCGGY 2 cut(s) 742, 943
Bse1I ACTGG 1 cut(s) 502
BseBI CCWGG 2 cut(s) 290, 310
BseCI ATCGAT 1 cut(s) 872
BseDI CCNNGG 4 cut(s) 217, 424, 606, 813
BseGI GGATG 3 cut(s) 285, 420, 1093
BseLI CCNNNNNNNGG 5 cut(s) 289, 309, 553, 607, 943
BseNI ACTGG 1 cut(s) 502
BsePI GCGCGC 1 cut(s) 451
BseRI GAGGAG 3 cut(s) 74, 77, 135
BseSI GKGCMC 3 cut(s) 601, 723, 822
BseXI GCAGC 4 cut(s) 380, 565, 569, 1084
BseYI CCCAGC 1 cut(s) 495
Bsh1236I CGCG 4 cut(s) 219, 231, 451, 453
BshFI GGCC 9 cut(s) 5, 460, 599, 611, 721, 742, 812, 820, 922
BshNI GGYRCC 2 cut(s) 572, 772
BshTI ACCGGT 1 cut(s) 943
BshVI ATCGAT 1 cut(s) 872
BsiHKAI GWGCWC 2 cut(s) 546, 849
BsiHKCI CYCGRG 2 cut(s) 268, 539
BsiSI CCGG 7 cut(s) 554, 595, 620, 723, 743, 931, 944
BsiWI CGTACG 1 cut(s) 204
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 5 cut(s) 289, 309, 553, 607, 943
BsmFI GGGAC 1 cut(s) 266
BsmI GAATGC 1 cut(s) 218
BsnI GGCC 9 cut(s) 5, 460, 599, 611, 721, 742, 812, 820, 922
BsoBI CYCGRG 2 cut(s) 268, 539
Bsp120I GGGCCC 3 cut(s) 597, 719, 818
Bsp1286I GDGCHC 5 cut(s) 546, 601, 723, 822, 849
Bsp143I GATC 5 cut(s) 561, 895, 955, 960, 985
BspANI GGCC 9 cut(s) 5, 460, 599, 611, 721, 742, 812, 820, 922
BspDI ATCGAT 1 cut(s) 872
BspFNI CGCG 4 cut(s) 219, 231, 451, 453
BspMAI CTGCAG 1 cut(s) 588
BspPI GGATC 3 cut(s) 556, 950, 955
BspT107I GGYRCC 2 cut(s) 572, 772
BsrBI CCGCTC 1 cut(s) 258
BsrFI RCCGGY 2 cut(s) 742, 943
BsrI ACTGG 1 cut(s) 502
BssAI RCCGGY 2 cut(s) 742, 943
BssECI CCNNGG 4 cut(s) 217, 424, 606, 813
BssHII GCGCGC 1 cut(s) 451
BssMI GATC 5 cut(s) 561, 895, 955, 960, 985
BssNAI GTATAC 1 cut(s) 235
BssNI GRCGYC 3 cut(s) 279, 318, 573
Bst1107I GTATAC 1 cut(s) 235
Bst2UI CCWGG 2 cut(s) 290, 310
Bst4CI ACNGT 4 cut(s) 428, 777, 793, 1090
Bst6I CTCTTC 1 cut(s) 116
BstACI GRCGYC 3 cut(s) 279, 318, 573
BstBAI YACGTR 2 cut(s) 627, 1054
BstC8I GCNNGC 5 cut(s) 366, 449, 453, 489, 682
BstDSI CCRYGG 2 cut(s) 217, 424
BstF5I GGATG 3 cut(s) 285, 420, 1093
BstFNI CGCG 4 cut(s) 219, 231, 451, 453
BstH2I RGCGCY 1 cut(s) 576
BstHHI GCGC 3 cut(s) 453, 455, 575
BstKTI GATC 5 cut(s) 564, 898, 958, 963, 988
BstMBI GATC 5 cut(s) 561, 895, 955, 960, 985
BstMWI GCNNNNNNNGC 2 cut(s) 228, 457
BstNI CCWGG 2 cut(s) 290, 310
BstNSI RCATGY 1 cut(s) 169
BstSCI CCNGG 5 cut(s) 288, 308, 552, 594, 721
BstSFI CTRYAG 1 cut(s) 584
BstSLI GKGCMC 3 cut(s) 601, 723, 822
BstUI CGCG 4 cut(s) 219, 231, 451, 453
BstV1I GCAGC 4 cut(s) 380, 565, 569, 1084
BstZ17I GTATAC 1 cut(s) 235
Bsu15I ATCGAT 1 cut(s) 872
BsuRI GGCC 9 cut(s) 5, 460, 599, 611, 721, 742, 812, 820, 922
BsuTUI ATCGAT 1 cut(s) 872
BtgI CCRYGG 2 cut(s) 217, 424
BtrI CACGTC 1 cut(s) 640
BtsCI GGATG 3 cut(s) 285, 420, 1093
BtsI GCAGTG 2 cut(s) 593, 988
BtsIMutI CAGTG 3 cut(s) 509, 593, 988
Cac8I GCNNGC 5 cut(s) 366, 449, 453, 489, 682
CfoI GCGC 3 cut(s) 453, 455, 575
Cfr10I RCCGGY 2 cut(s) 742, 943
Cfr42I CCGCGG 1 cut(s) 220
ClaI ATCGAT 1 cut(s) 872
Csp6I GTAC 2 cut(s) 205, 1051
CspAI ACCGGT 1 cut(s) 943
CviAII CATG 3 cut(s) 44, 166, 1076
CviQI GTAC 2 cut(s) 205, 1051
DinI GGCGCC 1 cut(s) 574
DpnI GATC 5 cut(s) 563, 897, 957, 962, 987
DpnII GATC 5 cut(s) 561, 895, 955, 960, 985
DrdI GACNNNNNNGTC 1 cut(s) 302
DseDI GACNNNNNNGTC 1 cut(s) 302
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
EciI GGCGGA 1 cut(s) 341
Ecl136II GAGCTC 1 cut(s) 847
Eco24I GRGCYC 4 cut(s) 601, 723, 822, 849
Eco53kI GAGCTC 1 cut(s) 847
Eco57I CTGAAG 1 cut(s) 1026
Eco72I CACGTG 1 cut(s) 627
Eco88I CYCGRG 2 cut(s) 268, 539
EcoICRI GAGCTC 1 cut(s) 847
EcoO109I RGGNCCY 1 cut(s) 818
EcoRII CCWGG 2 cut(s) 288, 308
EcoT38I GRGCYC 4 cut(s) 601, 723, 822, 849
EgeI GGCGCC 1 cut(s) 574
EheI GGCGCC 1 cut(s) 574
FaeI CATG 3 cut(s) 47, 169, 1079
FaiI YATR 8 cut(s) 17, 25, 45, 167, 235, 692, 699, 1077
FaqI GGGAC 1 cut(s) 266
FatI CATG 3 cut(s) 43, 165, 1075
FauI CCCGC 2 cut(s) 673, 677
FblI GTMKAC 1 cut(s) 234
Fnu4HI GCNGC 8 cut(s) 217, 220, 369, 558, 576, 579, 1073, 1110
FokI GGATG 3 cut(s) 272, 407, 1080
FriOI GRGCYC 4 cut(s) 601, 723, 822, 849
Fsp4HI GCNGC 8 cut(s) 217, 220, 369, 558, 576, 579, 1073, 1110
FspBI CTAG 1 cut(s) 965
GlaI GCGC 3 cut(s) 452, 454, 574
GluI GCNGC 8 cut(s) 217, 220, 369, 558, 576, 579, 1073, 1110
GsaI CCCAGC 1 cut(s) 499
GsuI CTGGAG 1 cut(s) 485
HaeII RGCGCY 1 cut(s) 576
HaeIII GGCC 9 cut(s) 5, 460, 599, 611, 721, 742, 812, 820, 922
HapII CCGG 7 cut(s) 554, 595, 620, 723, 743, 931, 944
HhaI GCGC 3 cut(s) 453, 455, 575
Hin1I GRCGYC 3 cut(s) 279, 318, 573
Hin1II CATG 3 cut(s) 47, 169, 1079
Hin6I GCGC 3 cut(s) 451, 453, 573
HinP1I GCGC 3 cut(s) 451, 453, 573
HincII GTYRAC 1 cut(s) 633
HindII GTYRAC 1 cut(s) 633
HinfI GANTC 2 cut(s) 674, 1140
HpaII CCGG 7 cut(s) 554, 595, 620, 723, 743, 931, 944
HphI GGTGA 5 cut(s) 143, 332, 683, 890, 1078
Hpy166II GTNNAC 4 cut(s) 171, 235, 633, 910
Hpy188I TCNGA 2 cut(s) 808, 960
Hpy188III TCNNGA 3 cut(s) 539, 953, 1027
Hpy8I GTNNAC 4 cut(s) 171, 235, 633, 910
Hpy99I CGWCG 8 cut(s) 245, 281, 299, 308, 323, 394, 572, 617
HpyAV CCTTC 4 cut(s) 125, 307, 1036, 1058
HpyCH4III ACNGT 4 cut(s) 428, 777, 793, 1090
HpyCH4IV ACGT 6 cut(s) 279, 318, 626, 639, 657, 1053
HpyCH4V TGCA 2 cut(s) 441, 586
HpyF10VI GCNNNNNNNGC 2 cut(s) 228, 457
HpySE526I ACGT 6 cut(s) 279, 318, 626, 639, 657, 1053
Hsp92I GRCGYC 3 cut(s) 279, 318, 573
Hsp92II CATG 3 cut(s) 47, 169, 1079
HspAI GCGC 3 cut(s) 451, 453, 573
KasI GGCGCC 1 cut(s) 572
KspI CCGCGG 1 cut(s) 220
Kzo9I GATC 5 cut(s) 561, 895, 955, 960, 985
LmnI GCTCC 9 cut(s) 251, 305, 408, 504, 541, 597, 844, 933, 1069
Lsp1109I GCAGC 4 cut(s) 380, 565, 569, 1084
LweI GCATC 3 cut(s) 450, 817, 1113
MaeI CTAG 1 cut(s) 965
MaeII ACGT 6 cut(s) 279, 318, 626, 639, 657, 1053
MaeIII GTNAC 1 cut(s) 671
MalI GATC 5 cut(s) 563, 897, 957, 962, 987
MbiI CCGCTC 1 cut(s) 258
MboI GATC 5 cut(s) 561, 895, 955, 960, 985
MboII GAAGA 7 cut(s) 90, 103, 218, 221, 403, 1019, 1075
MfeI CAATTG 2 cut(s) 838, 879
MhlI GDGCHC 5 cut(s) 546, 601, 723, 822, 849
MluCI AATT 8 cut(s) 129, 838, 879, 1030, 1058, 1099, 1128, 1153
Mly113I GGCGCC 1 cut(s) 573
MlyI GAGTC 1 cut(s) 668
MmeI TCCRAC 2 cut(s) 370, 786
MslI CAYNNNNRTG 1 cut(s) 164
MspA1I CMGCKG 2 cut(s) 219, 578
MspI CCGG 7 cut(s) 554, 595, 620, 723, 743, 931, 944
MspR9I CCNGG 5 cut(s) 290, 310, 554, 596, 723
MunI CAATTG 2 cut(s) 838, 879
Mva1269I GAATGC 1 cut(s) 218
MvaI CCWGG 2 cut(s) 290, 310
MvnI CGCG 4 cut(s) 219, 231, 451, 453
MwoI GCNNNNNNNGC 2 cut(s) 228, 457
NarI GGCGCC 1 cut(s) 573
NciI CCSGG 3 cut(s) 554, 596, 723
NdeII GATC 5 cut(s) 561, 895, 955, 960, 985
NlaIII CATG 3 cut(s) 47, 169, 1079
NmeAIII GCCGAG 3 cut(s) 631, 656, 838
NmuCI GTSAC 1 cut(s) 671
NspI RCATGY 1 cut(s) 169
PauI GCGCGC 1 cut(s) 451
PcsI WCGNNNNNNNCGW 3 cut(s) 249, 300, 324
PctI GAATGC 1 cut(s) 218
PfeI GAWTC 1 cut(s) 1140
Pfl23II CGTACG 1 cut(s) 204
PfoI TCCNGGA 1 cut(s) 288
PinAI ACCGGT 1 cut(s) 943
PkrI GCNGC 8 cut(s) 218, 221, 370, 559, 577, 580, 1074, 1111
PleI GAGTC 1 cut(s) 668
PluTI GGCGCC 1 cut(s) 576
PmaCI CACGTG 1 cut(s) 627
PmlI CACGTG 1 cut(s) 627
PpsI GAGTC 1 cut(s) 668
Ppu21I YACGTR 2 cut(s) 627, 1054
Psp124BI GAGCTC 1 cut(s) 849
Psp6I CCWGG 2 cut(s) 288, 308
PspCI CACGTG 1 cut(s) 627
PspFI CCCAGC 1 cut(s) 495
PspGI CCWGG 2 cut(s) 288, 308
PspLI CGTACG 1 cut(s) 204
PspOMI GGGCCC 3 cut(s) 597, 719, 818
PstI CTGCAG 1 cut(s) 588
PteI GCGCGC 1 cut(s) 451
RsaI GTAC 2 cut(s) 206, 1052
RsaNI GTAC 2 cut(s) 205, 1051
RseI CAYNNNNRTG 1 cut(s) 164
SacI GAGCTC 1 cut(s) 849
SacII CCGCGG 1 cut(s) 220
SatI GCNGC 8 cut(s) 217, 220, 369, 558, 576, 579, 1073, 1110
Sau3AI GATC 5 cut(s) 561, 895, 955, 960, 985
SchI GAGTC 1 cut(s) 668
ScrFI CCNGG 5 cut(s) 290, 310, 554, 596, 723
SduI GDGCHC 5 cut(s) 546, 601, 723, 822, 849
SfaNI GCATC 3 cut(s) 450, 817, 1113
SfcI CTRYAG 1 cut(s) 584
SfoI GGCGCC 1 cut(s) 574
Sfr303I CCGCGG 1 cut(s) 220
SgrBI CCGCGG 1 cut(s) 220
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 1025
SmoI CTYRAG 1 cut(s) 1025
Sse9I AATT 8 cut(s) 129, 838, 879, 1030, 1058, 1099, 1128, 1153
SspDI GGCGCC 1 cut(s) 572
SspMI CTAG 1 cut(s) 965
SstI GAGCTC 1 cut(s) 849
StyD4I CCNGG 5 cut(s) 288, 308, 552, 594, 721
TaaI ACNGT 4 cut(s) 428, 777, 793, 1090
TaiI ACGT 6 cut(s) 282, 321, 629, 642, 660, 1056
TaqI TCGA 3 cut(s) 276, 327, 872
TaqII GACCGA 1 cut(s) 1014
TasI AATT 8 cut(s) 129, 838, 879, 1030, 1058, 1099, 1128, 1153
TauI GCSGC 4 cut(s) 219, 222, 578, 1112
TfiI GAWTC 1 cut(s) 1140
TscAI CASTG 3 cut(s) 509, 593, 988
TseFI GTSAC 1 cut(s) 671
TseI GCWGC 4 cut(s) 368, 557, 578, 1072
Tsp45I GTSAC 1 cut(s) 671
TspDTI ATGAA 2 cut(s) 1082, 1132
TspGWI ACGGA 1 cut(s) 292
TspRI CASTG 3 cut(s) 509, 593, 988
XapI RAATTY 2 cut(s) 1058, 1153
XceI RCATGY 1 cut(s) 169
XmiI GTMKAC 1 cut(s) 234
XspI CTAG 1 cut(s) 965
ZraI GACGTC 2 cut(s) 280, 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.