MD12G1046500.v1.1

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001599
Physical Location & Seq
Reverse (-)
5303347 .. 5307135
3789 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1046500.v1.1.491

Sequence Viewer

Length: 981 bp
ATGGGTTCCCTCCCTCACATAGTAGAAGATTGCATGGGCGTCCTCCAAGTCTTTAGCGACGGTTCGATCAACCGTTGCTCTTTAACGGACATAAACTTCAACATTCCTGTCATTGATGACGGCTCCGTGGGCTTCAAAGACATCACTTTCGACAAAAAACACAAACTTTCCCTTCGTTTATACAAACCCAAATCGACAAACCAAGATTCCAAGCTCCCCGTGGTGTTCTACTTCCACGGTGGCGGCTTCTGTGTTGGCTCACACGTGTGGCCAAACTGCCACAATTGCTGCATCCGCCTGTCGTCGGGGCTGCAGGCACTTGTGGTATCTCCCGACTATAGGCTCGCTCCAGAGCACAAACTTCCGGCCGCGATTGACGATGCAGTGAGTGCAGTGGAGTGGCTGCAAAGGGAGGCTTCGGGAGAGAGCACGAATAACTGTGATGCATGGATTGGTGGTACAGCTGACTTCGACCGGGTTTTTATATTGGGTGACTCTTCAGGTGGGAATATGGCTCACCACCTGGCGGTTCGGCTCGCGACGGGGTCGATCAAGCCGGCTCCGATTAGGGTACGCGGGTTTGTGCTGCTGGCGCCATTTTTTGGTGGGGCGGAAAGGACAAAGTCAGAGGAAGGTCCTTGTGAGGCATTGTTGAGTTTGGAAATACTTGACAGATTTTGGAGGCTTTCGATGCCAGTAGGAGAAACCAGAGACCATCCAATGGTCAACCCATTTGGACCAAACAGTCCAAACCTTGAGAAGGTGGCCCTGGATCCCATCTTGGTTATCGTGGGTGGTAATGAACTGTTGAAAGATAGGGTGGAGAGCTACTGGAGAAAGTTAAAAGATTTGGGAAAGAAAATTGAGTATGTCGAATTTGAAGGAGAGCAACATGGCTTTTTAACTAATGATCCATACTCAGAAGTCTCAAATGAAGCTTTGCAAGTTATTAAAAGATTTATGACCGAAAACTCCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

327

Amino Acids

36.05

Weight (kDa)

5.6

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 56 - 177 9.5e-08 Carboxylesterase family
BD-FAE PF20434 60 - 177 8.6e-12 BD-FAE
Abhydrolase_3 PF07859 75 - 301 2.9e-58 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 744
AccB1I GGYRCC 1 cut(s) 592
AccB7I CCANNNNNTGG 2 cut(s) 602, 721
AccII CGCG 3 cut(s) 371, 539, 576
AciI CCGC 6 cut(s) 243, 295, 369, 527, 576, 611
AclWI GGATC 3 cut(s) 767, 780, 905
AcoI YGGCCR 2 cut(s) 269, 366
AcsI RAATTY 1 cut(s) 875
AcuI CTGAAG 1 cut(s) 483
AcvI CACGTG 1 cut(s) 265
AcyI GRCGYC 2 cut(s) 39, 593
AfaI GTAC 2 cut(s) 460, 573
AfiI CCNNNNNNNGG 6 cut(s) 304, 339, 526, 602, 721, 760
AflIII ACRYGT 2 cut(s) 262, 264
AgsI TTSAA 4 cut(s) 100, 136, 811, 881
AjnI CCWGG 2 cut(s) 522, 768
AleI CACNNNNGTG 1 cut(s) 265
AluBI AGCT 4 cut(s) 214, 464, 828, 938
AluI AGCT 4 cut(s) 214, 464, 828, 938
Alw21I GWGCWC 2 cut(s) 357, 431
Alw26I GTCTC 2 cut(s) 705, 931
AlwI GGATC 3 cut(s) 767, 780, 905
AoxI GGCC 3 cut(s) 269, 366, 765
ApeKI GCWGC 4 cut(s) 288, 310, 403, 586
ApoI RAATTY 1 cut(s) 875
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AspLEI GCGC 1 cut(s) 595
AspS9I GGNCC 3 cut(s) 635, 737, 766
AsuC2I CCSGG 1 cut(s) 476
AsuHPI GGTGA 2 cut(s) 503, 509
AvaII GGWCC 2 cut(s) 635, 737
BaeI ACNNNNGTAYC 2 cut(s) 563, 596
BalI TGGCCA 1 cut(s) 271
BamHI GGATCC 1 cut(s) 772
BanI GGYRCC 1 cut(s) 592
BbrPI CACGTG 1 cut(s) 265
Bbv12I GWGCWC 2 cut(s) 357, 431
BbvI GCAGC 4 cut(s) 275, 297, 390, 573
BccI CCATC 2 cut(s) 723, 785
BceAI ACGGC 1 cut(s) 136
BciT130I CCWGG 2 cut(s) 524, 770
BcnI CCSGG 1 cut(s) 476
BcoDI GTCTC 2 cut(s) 705, 931
BfmI CTRYAG 2 cut(s) 311, 337
BfoI RGCGCY 1 cut(s) 596
BisI GCNGC 6 cut(s) 244, 289, 311, 369, 404, 587
BlsI GCNGC 6 cut(s) 245, 290, 312, 370, 405, 588
Bme1390I CCNGG 3 cut(s) 476, 524, 770
Bme18I GGWCC 2 cut(s) 635, 737
BmgT120I GGNCC 3 cut(s) 635, 737, 766
BmiI GGNNCC 5 cut(s) 7, 124, 561, 594, 774
BmrFI CCNGG 3 cut(s) 476, 524, 770
BmsI GCATC 4 cut(s) 300, 370, 433, 681
BpmI CTGGAG 2 cut(s) 333, 853
BpuEI CTTGAG 1 cut(s) 776
BpuMI CCSGG 1 cut(s) 476
BsaAI YACGTR 1 cut(s) 265
BsaHI GRCGYC 2 cut(s) 39, 593
BsaI GGTCTC 1 cut(s) 705
BsaJI CCNNGG 4 cut(s) 126, 219, 235, 768
Bsc4I CCNNNNNNNGG 6 cut(s) 304, 339, 526, 602, 721, 760
Bse118I RCCGGY 1 cut(s) 556
Bse1I ACTGG 2 cut(s) 695, 836
BseBI CCWGG 2 cut(s) 524, 770
BseDI CCNNGG 4 cut(s) 126, 219, 235, 768
BseGI GGATG 2 cut(s) 291, 715
BseLI CCNNNNNNNGG 6 cut(s) 304, 339, 526, 602, 721, 760
BseMII CTCAG 1 cut(s) 933
BseNI ACTGG 2 cut(s) 695, 836
BseX3I CGGCCG 1 cut(s) 366
BseXI GCAGC 4 cut(s) 275, 297, 390, 573
BsgI GTGCAG 1 cut(s) 411
Bsh1236I CGCG 3 cut(s) 371, 539, 576
Bsh1285I CGRYCG 2 cut(s) 369, 475
BshFI GGCC 3 cut(s) 271, 368, 767
BshNI GGYRCC 1 cut(s) 592
BsiEI CGRYCG 2 cut(s) 369, 475
BsiHKAI GWGCWC 2 cut(s) 357, 431
BsiSI CCGG 3 cut(s) 365, 475, 557
BslI CCNNNNNNNGG 6 cut(s) 304, 339, 526, 602, 721, 760
BsmAI GTCTC 2 cut(s) 705, 931
BsnI GGCC 3 cut(s) 271, 368, 767
Bso31I GGTCTC 1 cut(s) 705
Bsp1286I GDGCHC 2 cut(s) 357, 431
Bsp143I GATC 4 cut(s) 66, 549, 772, 910
Bsp68I TCGCGA 1 cut(s) 539
BspACI CCGC 6 cut(s) 243, 295, 369, 527, 576, 611
BspANI GGCC 3 cut(s) 271, 368, 767
BspCNI CTCAG 1 cut(s) 932
BspFNI CGCG 3 cut(s) 371, 539, 576
BspLI GGNNCC 5 cut(s) 7, 124, 561, 594, 774
BspMAI CTGCAG 1 cut(s) 315
BspPI GGATC 3 cut(s) 767, 780, 905
BspT107I GGYRCC 1 cut(s) 592
BspTNI GGTCTC 1 cut(s) 705
BsrFI RCCGGY 1 cut(s) 556
BsrI ACTGG 2 cut(s) 695, 836
BssAI RCCGGY 1 cut(s) 556
BssECI CCNNGG 4 cut(s) 126, 219, 235, 768
BssMI GATC 4 cut(s) 66, 549, 772, 910
BssNI GRCGYC 2 cut(s) 39, 593
Bst2UI CCWGG 2 cut(s) 524, 770
Bst4CI ACNGT 6 cut(s) 62, 74, 239, 440, 746, 807
Bst6I CTCTTC 1 cut(s) 502
BstACI GRCGYC 2 cut(s) 39, 593
BstAPI GCANNNNNTGC 1 cut(s) 389
BstBAI YACGTR 1 cut(s) 265
BstC8I GCNNGC 5 cut(s) 315, 345, 537, 558, 591
BstDEI CTNAG 1 cut(s) 919
BstDSI CCRYGG 3 cut(s) 126, 219, 235
BstENI CCTNNNNNAGG 1 cut(s) 758
BstF5I GGATG 2 cut(s) 291, 715
BstFNI CGCG 3 cut(s) 371, 539, 576
BstH2I RGCGCY 1 cut(s) 596
BstHHI GCGC 1 cut(s) 595
BstKTI GATC 4 cut(s) 69, 552, 775, 913
BstMAI GTCTC 2 cut(s) 705, 931
BstMBI GATC 4 cut(s) 66, 549, 772, 910
BstMCI CGRYCG 2 cut(s) 369, 475
BstMWI GCNNNNNNNGC 6 cut(s) 129, 285, 294, 389, 592, 691
BstNI CCWGG 2 cut(s) 524, 770
BstSCI CCNGG 3 cut(s) 474, 522, 768
BstSFI CTRYAG 2 cut(s) 311, 337
BstUI CGCG 3 cut(s) 371, 539, 576
BstV1I GCAGC 4 cut(s) 275, 297, 390, 573
BstX2I RGATCY 1 cut(s) 772
BstYI RGATCY 1 cut(s) 772
BstZI CGGCCG 1 cut(s) 366
BsuRI GGCC 3 cut(s) 271, 368, 767
BtgI CCRYGG 3 cut(s) 126, 219, 235
BtsCI GGATG 2 cut(s) 291, 715
BtsI GCAGTG 2 cut(s) 390, 399
BtsIMutI CAGTG 2 cut(s) 390, 399
BtuMI TCGCGA 1 cut(s) 539
Cac8I GCNNGC 5 cut(s) 315, 345, 537, 558, 591
CfoI GCGC 1 cut(s) 595
Cfr10I RCCGGY 1 cut(s) 556
Cfr13I GGNCC 3 cut(s) 635, 737, 766
CseI GACGC 1 cut(s) 28
Csp6I GTAC 2 cut(s) 459, 572
CviAII CATG 3 cut(s) 34, 447, 893
CviQI GTAC 2 cut(s) 459, 572
DdeI CTNAG 1 cut(s) 919
DinI GGCGCC 1 cut(s) 594
DpnI GATC 4 cut(s) 68, 551, 774, 912
DpnII GATC 4 cut(s) 66, 549, 772, 910
DrdI GACNNNNNNGTC 1 cut(s) 744
DseDI GACNNNNNNGTC 1 cut(s) 744
EaeI YGGCCR 2 cut(s) 269, 366
EagI CGGCCG 1 cut(s) 366
Eam1104I CTCTTC 1 cut(s) 502
EarI CTCTTC 1 cut(s) 502
EciI GGCGGA 2 cut(s) 284, 626
EclXI CGGCCG 1 cut(s) 366
Eco31I GGTCTC 1 cut(s) 705
Eco47I GGWCC 2 cut(s) 635, 737
Eco52I CGGCCG 1 cut(s) 366
Eco57I CTGAAG 1 cut(s) 483
Eco72I CACGTG 1 cut(s) 265
EcoNI CCTNNNNNAGG 1 cut(s) 758
EcoO109I RGGNCCY 1 cut(s) 635
EcoRII CCWGG 2 cut(s) 522, 768
EcoT22I ATGCAT 1 cut(s) 448
EgeI GGCGCC 1 cut(s) 594
EheI GGCGCC 1 cut(s) 594
FaeI CATG 3 cut(s) 37, 450, 896
FalI AAGNNNNNCTT 2 cut(s) 400, 432
FatI CATG 3 cut(s) 33, 446, 892
FauI CCCGC 1 cut(s) 569
Fnu4HI GCNGC 6 cut(s) 244, 289, 311, 369, 404, 587
FokI GGATG 2 cut(s) 278, 702
Fsp4HI GCNGC 6 cut(s) 244, 289, 311, 369, 404, 587
GlaI GCGC 1 cut(s) 594
GluI GCNGC 6 cut(s) 244, 289, 311, 369, 404, 587
GsuI CTGGAG 2 cut(s) 333, 853
HaeII RGCGCY 1 cut(s) 596
HaeIII GGCC 3 cut(s) 271, 368, 767
HapII CCGG 3 cut(s) 365, 475, 557
HgaI GACGC 1 cut(s) 28
HhaI GCGC 1 cut(s) 595
Hin1I GRCGYC 2 cut(s) 39, 593
Hin1II CATG 3 cut(s) 37, 450, 896
Hin6I GCGC 1 cut(s) 593
HinP1I GCGC 1 cut(s) 593
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HindIII AAGCTT 1 cut(s) 936
HinfI GANTC 2 cut(s) 206, 494
HpaII CCGG 3 cut(s) 365, 475, 557
HphI GGTGA 2 cut(s) 503, 509
Hpy166II GTNNAC 1 cut(s) 727
Hpy188I TCNGA 3 cut(s) 564, 628, 922
Hpy188III TCNNGA 4 cut(s) 332, 350, 420, 538
Hpy8I GTNNAC 1 cut(s) 727
Hpy99I CGWCG 3 cut(s) 62, 307, 544
HpyAV CCTTC 4 cut(s) 182, 626, 754, 875
HpyCH4III ACNGT 6 cut(s) 62, 74, 239, 440, 746, 807
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 8 cut(s) 33, 291, 313, 383, 392, 406, 446, 943
HpyF10VI GCNNNNNNNGC 6 cut(s) 129, 285, 294, 389, 592, 691
HpyF3I CTNAG 1 cut(s) 919
HpySE526I ACGT 1 cut(s) 264
Hsp92I GRCGYC 2 cut(s) 39, 593
Hsp92II CATG 3 cut(s) 37, 450, 896
HspAI GCGC 1 cut(s) 593
KasI GGCGCC 1 cut(s) 592
KroI GCCGGC 1 cut(s) 556
KroNI GCCGGC 1 cut(s) 558
Kzo9I GATC 4 cut(s) 66, 549, 772, 910
LmnI GCTCC 4 cut(s) 128, 219, 352, 565
Lsp1109I GCAGC 4 cut(s) 275, 297, 390, 573
LweI GCATC 4 cut(s) 300, 370, 433, 681
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 1 cut(s) 491
MalI GATC 4 cut(s) 68, 551, 774, 912
MboI GATC 4 cut(s) 66, 549, 772, 910
MboII GAAGA 2 cut(s) 38, 489
MfeI CAATTG 1 cut(s) 283
MflI RGATCY 1 cut(s) 772
MhlI GDGCHC 2 cut(s) 357, 431
MlsI TGGCCA 1 cut(s) 271
MluCI AATT 3 cut(s) 283, 861, 875
MluNI TGGCCA 1 cut(s) 271
Mly113I GGCGCC 1 cut(s) 593
MlyI GAGTC 1 cut(s) 488
MnlI CCTC 7 cut(s) 20, 24, 53, 406, 622, 637, 675
Mox20I TGGCCA 1 cut(s) 271
Mph1103I ATGCAT 1 cut(s) 448
MroNI GCCGGC 1 cut(s) 556
MscI TGGCCA 1 cut(s) 271
MseI TTAA 4 cut(s) 83, 842, 902, 951
MslI CAYNNNNRTG 1 cut(s) 265
Msp20I TGGCCA 1 cut(s) 271
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 3 cut(s) 365, 475, 557
MspR9I CCNGG 3 cut(s) 476, 524, 770
MunI CAATTG 1 cut(s) 283
MvaI CCWGG 2 cut(s) 524, 770
MvnI CGCG 3 cut(s) 371, 539, 576
MwoI GCNNNNNNNGC 6 cut(s) 129, 285, 294, 389, 592, 691
NaeI GCCGGC 1 cut(s) 558
NarI GGCGCC 1 cut(s) 593
NciI CCSGG 1 cut(s) 476
NdeII GATC 4 cut(s) 66, 549, 772, 910
NgoMIV GCCGGC 1 cut(s) 556
NlaIII CATG 3 cut(s) 37, 450, 896
NlaIV GGNNCC 5 cut(s) 7, 124, 561, 594, 774
NmuCI GTSAC 1 cut(s) 491
NruI TCGCGA 1 cut(s) 539
NsiI ATGCAT 1 cut(s) 448
OliI CACNNNNGTG 1 cut(s) 265
PdiI GCCGGC 1 cut(s) 558
PfeI GAWTC 1 cut(s) 206
PflFI GACNNNGTC 2 cut(s) 544, 622
PflMI CCANNNNNTGG 2 cut(s) 602, 721
PkrI GCNGC 6 cut(s) 245, 290, 312, 370, 405, 588
PleI GAGTC 1 cut(s) 488
PluTI GGCGCC 1 cut(s) 596
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
PpsI GAGTC 1 cut(s) 488
Ppu21I YACGTR 1 cut(s) 265
PpuMI RGGWCCY 1 cut(s) 635
Psp5II RGGWCCY 1 cut(s) 635
Psp6I CCWGG 2 cut(s) 522, 768
PspCI CACGTG 1 cut(s) 265
PspGI CCWGG 2 cut(s) 522, 768
PspN4I GGNNCC 5 cut(s) 7, 124, 561, 594, 774
PspPI GGNCC 3 cut(s) 635, 737, 766
PspPPI RGGWCCY 1 cut(s) 635
PstI CTGCAG 1 cut(s) 315
PsuI RGATCY 1 cut(s) 772
PsyI GACNNNGTC 2 cut(s) 544, 622
PvuII CAGCTG 1 cut(s) 464
RruI TCGCGA 1 cut(s) 539
RsaI GTAC 2 cut(s) 460, 573
RsaNI GTAC 2 cut(s) 459, 572
RseI CAYNNNNRTG 1 cut(s) 265
SaqAI TTAA 4 cut(s) 83, 842, 902, 951
SatI GCNGC 6 cut(s) 244, 289, 311, 369, 404, 587
Sau3AI GATC 4 cut(s) 66, 549, 772, 910
Sau96I GGNCC 3 cut(s) 635, 737, 766
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 3 cut(s) 476, 524, 770
SduI GDGCHC 2 cut(s) 357, 431
SfaNI GCATC 4 cut(s) 300, 370, 433, 681
SfcI CTRYAG 2 cut(s) 311, 337
SfoI GGCGCC 1 cut(s) 594
SinI GGWCC 2 cut(s) 635, 737
SmiMI CAYNNNNRTG 1 cut(s) 265
SmlI CTYRAG 1 cut(s) 755
SmoI CTYRAG 1 cut(s) 755
Sse9I AATT 3 cut(s) 283, 861, 875
SsiI CCGC 6 cut(s) 243, 295, 369, 527, 576, 611
SspDI GGCGCC 1 cut(s) 592
StyD4I CCNGG 3 cut(s) 474, 522, 768
TaaI ACNGT 6 cut(s) 62, 74, 239, 440, 746, 807
TaiI ACGT 1 cut(s) 267
TaqI TCGA 7 cut(s) 65, 150, 194, 471, 548, 689, 873
TaqII GACCGA 1 cut(s) 980
TasI AATT 3 cut(s) 283, 861, 875
TauI GCSGC 2 cut(s) 246, 371
TfiI GAWTC 1 cut(s) 206
Tru1I TTAA 4 cut(s) 83, 842, 902, 951
Tru9I TTAA 4 cut(s) 83, 842, 902, 951
TscAI CASTG 2 cut(s) 390, 399
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 4 cut(s) 288, 310, 403, 586
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 2 cut(s) 816, 948
TspGWI ACGGA 2 cut(s) 101, 115
TspRI CASTG 2 cut(s) 390, 399
Tth111I GACNNNGTC 2 cut(s) 544, 622
Van91I CCANNNNNTGG 2 cut(s) 602, 721
VpaK11BI GGWCC 2 cut(s) 635, 737
XagI CCTNNNNNAGG 1 cut(s) 758
XapI RAATTY 1 cut(s) 875
XcmI CCANNNNNNNNNTGG 1 cut(s) 217
Zsp2I ATGCAT 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.