Rorug05G0246700

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001537
Physical Location & Seq
Forward (+)
26551334 .. 26557385
6052 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0246700.1

Sequence Viewer

Length: 1548 bp
ATGAACGAGAAGGCCAACGTCACCAAGGAGCTCAACGCCAAGCACAGAAAGATACTGGAAGGACTTCTTAAATTGCCAGAGAATAGGGAATGTGCTGACTGCAAAGCCAAGGGTCCAAGATGGGCCAGTGTGAACTTAGGTATCTTTGTATGCATGCAGTGTTCTGGGATCCACAGAAGTCTCGGGGTACACATATCCAAGGTGAGGTCTGCTACCTTGGACACATGGCTACCAGAGCAGGTGGCATTTATTCAATCAATGGGAAATGAAAGGGCAAATAGTTACTGGGAAGCAGAACTACCTCCAAACTATGACAGAGTTGGTATTGAAAATTTTATTCGTGCAAAGTATGAAGATAAGAGATGGGTCGCTAAAGATGGAAAACCAAAATCACCTTCTAGAGGACGTGAGGAAAATGCTTCCACACATTGGCAGAGACCTGTTGAAAGCAGTGGGCATGGGTATATCACAAATTCTGAAAAGAAATTTGAGGAAAGGAAGAACATACAAACAGCTAGTAGAAGAGACAGTGTTCCTGCTGGAAGAATCAGTCTTCCTGTTCCTCCTAAAGGGCCTGATCATGTTGTACAGGTCCCAAAACCACAAAATGTTCAGAAAACAGAACCAGCAGTTCCACAGGCTGAAGCAACAAAGCCAGCTGCTGAAGTTGCTTCTGCTGCGCCTCCAAAAGTTGATTATGCTACTGACCTTTTCGGCATGCTCTCAGTAGAAAGTCCCAGTGAGAATAGCTCAGATGCAGCACCAGCTGACGATAATGATTGGGCAGGTTTCCAGTCGGCTAATGAAGCATCAAAAATTGAGAAAACCAGTCCACCAAATGCAGCAGAAGGCAGTAGCAAGTCTGCATCAGGAATCGAGGATTTATTTGCAGATTCACTGTCATTAACACCTGTTGCAGAGAAACCCAAGAAAGATGTTAAAAATGATATTATGAGTCTTTTTGAGAAGTCCAATATGGTGTCACCATTCGCTATGCATCAACAGCAACTCACAATGTTGGCTCAGCAGCAGTCTCTTTTCATGGCTGCAGCTAAATCTGCTGGTGGGGACCAAAAGTTTCCGGGCACTACACAACAACCTGTTTTGAATGGAAGCAATTTACCTTCCCAACAGCAACTTGCAATGCTGGCTCAGCAGCAGTCTTTTCTCATGGCTGCTGCAGCTAAATCTGCTGGCATGGACCAAAAGTTCCCTGGCGTTGCTCAACAGCCCGTGTCAAATGGAAGCAGTTTACCCTCCCAAAGTTGGCCAAACATTGGCTACCAAATTCCTGGAATGATGATGCCTATAGATGGACAGGCAAGGACAGCGGGACCGGCAAATCCAACGGGAAGTTCTGTTCCATATCCAACGTCTAGCTTTTATTCTATGCCCCAAGTTAGCCCAGCTAATGGCCTTACAAATGCTGGAGTGAACAAAACTCAGTCACCGGCTCCAACATCGGCTGCAGCTTCTTCTACCCCAATACAAAAGGGAAAAGATTATGATTTCTCTTCCTTAACACAAGGGATGTTCGCAAAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

515

Amino Acids

55.42

Weight (kDa)

8.62

Isoelectric Point (pI)

60.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 17 - 125 5.9e-42 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 229
Acc36I ACCTGC 2 cut(s) 229, 776
AccB7I CCANNNNNTGG 3 cut(s) 429, 1277, 1412
AciI CCGC 1 cut(s) 1331
AclWI GGATC 2 cut(s) 163, 176
AcoI YGGCCR 1 cut(s) 1268
AcsI RAATTY 4 cut(s) 331, 472, 485, 1287
AcuI CTGAAG 2 cut(s) 663, 684
AfaI GTAC 2 cut(s) 189, 588
AfiI CCNNNNNNNGG 8 cut(s) 204, 401, 429, 569, 1266, 1277, 1313, 1412
AgsI TTSAA 4 cut(s) 254, 329, 446, 1108
AjiI CACGTC 1 cut(s) 407
AjnI CCWGG 2 cut(s) 1213, 1291
AjuI GAANNNNNNNTTGG 4 cut(s) 379, 411, 1339, 1371
AloI GAACNNNNNNTCC 4 cut(s) 1193, 1225, 1344, 1376
Alw21I GWGCWC 1 cut(s) 33
Alw26I GTCTC 4 cut(s) 185, 430, 519, 1038
AlwI GGATC 2 cut(s) 163, 176
AlwNI CAGNNNCTG 1 cut(s) 662
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 5 cut(s) 12, 123, 572, 1268, 1414
ApoI RAATTY 4 cut(s) 331, 472, 485, 1287
Asp700I GAANNNNTTC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 682
AspS9I GGNCC 7 cut(s) 113, 123, 572, 592, 1069, 1201, 1334
AsuC2I CCSGG 1 cut(s) 1083
AsuHPI GGTGA 5 cut(s) 13, 214, 384, 975, 1440
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 5 cut(s) 113, 592, 1069, 1201, 1334
BaeGI GKGCMC 1 cut(s) 1088
BaeI ACNNNNGTAYC 2 cut(s) 124, 157
BalI TGGCCA 1 cut(s) 1270
BamHI GGATCC 1 cut(s) 168
BanII GRGCYC 1 cut(s) 33
BarI GAAGNNNNNNTAC 2 cut(s) 282, 314
BbsI GAAGAC 1 cut(s) 545
Bbv12I GWGCWC 1 cut(s) 33
BccI CCATC 4 cut(s) 114, 357, 371, 1307
BciT130I CCWGG 2 cut(s) 1215, 1293
BclI TGATCA 1 cut(s) 577
BcnI CCSGG 1 cut(s) 1083
BcoDI GTCTC 4 cut(s) 185, 430, 519, 1038
BfaI CTAG 3 cut(s) 399, 516, 1377
BfmI CTRYAG 4 cut(s) 1047, 1179, 1308, 1467
BfuAI ACCTGC 2 cut(s) 229, 776
BlpI GCTNAGC 2 cut(s) 1023, 1152
Bme1390I CCNGG 3 cut(s) 1083, 1215, 1293
Bme18I GGWCC 5 cut(s) 113, 592, 1069, 1201, 1334
BmeT110I CYCGRG 1 cut(s) 182
BmgBI CACGTC 1 cut(s) 407
BmgT120I GGNCC 7 cut(s) 113, 123, 572, 592, 1069, 1201, 1334
BmiI GGNNCC 6 cut(s) 114, 170, 594, 1070, 1335, 1455
BmrFI CCNGG 3 cut(s) 1083, 1215, 1293
BmrI ACTGGG 2 cut(s) 295, 732
BmsI GCATC 5 cut(s) 745, 818, 875, 1006, 1293
BmuI ACTGGG 2 cut(s) 295, 732
BpiI GAAGAC 1 cut(s) 545
BplI GAGNNNNNCTC 2 cut(s) 734, 766
BpmI CTGGAG 1 cut(s) 1449
Bpu1102I GCTNAGC 2 cut(s) 1023, 1152
BpuMI CCSGG 1 cut(s) 1083
BsaI GGTCTC 1 cut(s) 430
BsaJI CCNNGG 5 cut(s) 24, 108, 198, 216, 1213
Bsc4I CCNNNNNNNGG 8 cut(s) 204, 401, 429, 569, 1266, 1277, 1313, 1412
Bse118I RCCGGY 2 cut(s) 1336, 1450
Bse1I ACTGG 6 cut(s) 60, 126, 290, 738, 793, 828
Bse3DI GCAATG 1 cut(s) 1149
BseBI CCWGG 2 cut(s) 1215, 1293
BseDI CCNNGG 5 cut(s) 24, 108, 198, 216, 1213
BseGI GGATG 1 cut(s) 1536
BseLI CCNNNNNNNGG 8 cut(s) 204, 401, 429, 569, 1266, 1277, 1313, 1412
BseMI GCAATG 1 cut(s) 1149
BseMII CTCAG 5 cut(s) 738, 765, 1037, 1166, 1457
BseNI ACTGG 6 cut(s) 60, 126, 290, 738, 793, 828
BseSI GKGCMC 1 cut(s) 1088
BseYI CCCAGC 1 cut(s) 1405
BshFI GGCC 5 cut(s) 14, 125, 574, 1270, 1416
BsiHKAI GWGCWC 1 cut(s) 33
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 3 cut(s) 1082, 1337, 1451
BslFI GGGAC 4 cut(s) 578, 720, 1082, 1347
BslI CCNNNNNNNGG 8 cut(s) 204, 401, 429, 569, 1266, 1277, 1313, 1412
BsmAI GTCTC 4 cut(s) 185, 430, 519, 1038
BsmFI GGGAC 4 cut(s) 578, 720, 1082, 1347
BsnI GGCC 5 cut(s) 14, 125, 574, 1270, 1416
Bso31I GGTCTC 1 cut(s) 430
BsoBI CYCGRG 1 cut(s) 182
Bsp1286I GDGCHC 2 cut(s) 33, 1088
Bsp1407I TGTACA 1 cut(s) 586
Bsp143I GATC 2 cut(s) 168, 577
Bsp1720I GCTNAGC 2 cut(s) 1023, 1152
BspACI CCGC 1 cut(s) 1331
BspANI GGCC 5 cut(s) 14, 125, 574, 1270, 1416
BspCNI CTCAG 5 cut(s) 737, 764, 1036, 1165, 1456
BspLI GGNNCC 6 cut(s) 114, 170, 594, 1070, 1335, 1455
BspMAI CTGCAG 3 cut(s) 1051, 1183, 1471
BspMI ACCTGC 2 cut(s) 229, 776
BspPI GGATC 2 cut(s) 163, 176
BspTNI GGTCTC 1 cut(s) 430
BsrDI GCAATG 1 cut(s) 1149
BsrFI RCCGGY 2 cut(s) 1336, 1450
BsrGI TGTACA 1 cut(s) 586
BsrI ACTGG 6 cut(s) 60, 126, 290, 738, 793, 828
BssAI RCCGGY 2 cut(s) 1336, 1450
BssECI CCNNGG 5 cut(s) 24, 108, 198, 216, 1213
BssMI GATC 2 cut(s) 168, 577
BssT1I CCWWGG 4 cut(s) 24, 108, 198, 216
Bst2UI CCWGG 2 cut(s) 1215, 1293
Bst4CI ACNGT 3 cut(s) 530, 900, 1545
Bst6I CTCTTC 2 cut(s) 517, 1519
BstAUI TGTACA 1 cut(s) 586
BstC8I GCNNGC 5 cut(s) 155, 657, 719, 1149, 1195
BstDEI CTNAG 6 cut(s) 136, 724, 751, 1023, 1152, 1443
BstENI CCTNNNNNAGG 2 cut(s) 399, 567
BstF5I GGATG 1 cut(s) 1536
BstHHI GCGC 1 cut(s) 682
BstKTI GATC 2 cut(s) 171, 580
BstMAI GTCTC 4 cut(s) 185, 430, 519, 1038
BstMBI GATC 2 cut(s) 168, 577
BstNI CCWGG 2 cut(s) 1215, 1293
BstNSI RCATGY 2 cut(s) 157, 721
BstSCI CCNGG 3 cut(s) 1081, 1213, 1291
BstSFI CTRYAG 4 cut(s) 1047, 1179, 1308, 1467
BstSLI GKGCMC 1 cut(s) 1088
BstV2I GAAGAC 1 cut(s) 545
BstX2I RGATCY 1 cut(s) 168
BstXI CCANNNNNNTGG 1 cut(s) 1292
BstYI RGATCY 1 cut(s) 168
BsuRI GGCC 5 cut(s) 14, 125, 574, 1270, 1416
BtrI CACGTC 1 cut(s) 407
BtsCI GGATG 1 cut(s) 1536
BtsI GCAGTG 2 cut(s) 164, 457
BtsIMutI CAGTG 6 cut(s) 133, 164, 457, 535, 745, 896
BveI ACCTGC 2 cut(s) 229, 776
Cac8I GCNNGC 5 cut(s) 155, 657, 719, 1149, 1195
CaiI CAGNNNCTG 1 cut(s) 662
CfoI GCGC 1 cut(s) 682
Cfr10I RCCGGY 2 cut(s) 1336, 1450
Cfr13I GGNCC 7 cut(s) 113, 123, 572, 592, 1069, 1201, 1334
Csp6I GTAC 2 cut(s) 188, 587
CviAII CATG 8 cut(s) 154, 225, 458, 581, 718, 1042, 1171, 1198
CviQI GTAC 2 cut(s) 188, 587
DdeI CTNAG 6 cut(s) 136, 724, 751, 1023, 1152, 1443
DpnI GATC 2 cut(s) 170, 579
DpnII GATC 2 cut(s) 168, 577
EaeI YGGCCR 1 cut(s) 1268
Eam1104I CTCTTC 2 cut(s) 517, 1519
EarI CTCTTC 2 cut(s) 517, 1519
Ecl136II GAGCTC 1 cut(s) 31
Eco130I CCWWGG 4 cut(s) 24, 108, 198, 216
Eco24I GRGCYC 1 cut(s) 33
Eco31I GGTCTC 1 cut(s) 430
Eco47I GGWCC 5 cut(s) 113, 592, 1069, 1201, 1334
Eco53kI GAGCTC 1 cut(s) 31
Eco57I CTGAAG 2 cut(s) 663, 684
Eco88I CYCGRG 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 31
EcoNI CCTNNNNNAGG 2 cut(s) 399, 567
EcoO109I RGGNCCY 2 cut(s) 572, 592
EcoRII CCWGG 2 cut(s) 1213, 1291
EcoT14I CCWWGG 4 cut(s) 24, 108, 198, 216
EcoT22I ATGCAT 2 cut(s) 155, 999
EcoT38I GRGCYC 1 cut(s) 33
ErhI CCWWGG 4 cut(s) 24, 108, 198, 216
FaeI CATG 8 cut(s) 157, 228, 461, 584, 721, 1045, 1174, 1201
FalI AAGNNNNNCTT 2 cut(s) 51, 83
FaqI GGGAC 4 cut(s) 578, 720, 1082, 1347
FatI CATG 8 cut(s) 153, 224, 457, 580, 717, 1041, 1170, 1197
FauI CCCGC 1 cut(s) 1324
FbaI TGATCA 1 cut(s) 577
FokI GGATG 1 cut(s) 1543
FriOI GRGCYC 1 cut(s) 33
FspBI CTAG 3 cut(s) 399, 516, 1377
GlaI GCGC 1 cut(s) 681
GsaI CCCAGC 1 cut(s) 1409
GsuI CTGGAG 1 cut(s) 1449
HaeIII GGCC 5 cut(s) 14, 125, 574, 1270, 1416
HapII CCGG 3 cut(s) 1082, 1337, 1451
HhaI GCGC 1 cut(s) 682
Hin1II CATG 8 cut(s) 157, 228, 461, 584, 721, 1045, 1174, 1201
Hin6I GCGC 1 cut(s) 680
HinP1I GCGC 1 cut(s) 680
HinfI GANTC 4 cut(s) 546, 873, 893, 955
HpaII CCGG 3 cut(s) 1082, 1337, 1451
HphI GGTGA 5 cut(s) 13, 214, 384, 975, 1440
Hpy166II GTNNAC 5 cut(s) 133, 190, 833, 1253, 1435
Hpy188I TCNGA 3 cut(s) 478, 615, 754
Hpy188III TCNNGA 2 cut(s) 399, 870
Hpy8I GTNNAC 5 cut(s) 133, 190, 833, 1253, 1435
HpyAV CCTTC 5 cut(s) 4, 53, 405, 842, 1134
HpyCH4III ACNGT 3 cut(s) 530, 900, 1545
HpyCH4IV ACGT 3 cut(s) 18, 406, 1373
HpyF3I CTNAG 6 cut(s) 136, 724, 751, 1023, 1152, 1443
HpySE526I ACGT 3 cut(s) 18, 406, 1373
Hsp92II CATG 8 cut(s) 157, 228, 461, 584, 721, 1045, 1174, 1201
HspAI GCGC 1 cut(s) 680
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 2 cut(s) 168, 577
LmnI GCTCC 2 cut(s) 28, 1459
LweI GCATC 5 cut(s) 745, 818, 875, 1006, 1293
MaeI CTAG 3 cut(s) 399, 516, 1377
MaeII ACGT 3 cut(s) 18, 406, 1373
MaeIII GTNAC 4 cut(s) 19, 281, 981, 1446
MalI GATC 2 cut(s) 170, 579
MboI GATC 2 cut(s) 168, 577
MboII GAAGA 7 cut(s) 365, 511, 534, 545, 555, 1467, 1506
MflI RGATCY 1 cut(s) 168
MhlI GDGCHC 2 cut(s) 33, 1088
MlsI TGGCCA 1 cut(s) 1270
MluCI AATT 7 cut(s) 71, 331, 472, 485, 816, 1117, 1287
MluNI TGGCCA 1 cut(s) 1270
MlyI GAGTC 1 cut(s) 964
MmeI TCCRAC 3 cut(s) 1370, 1394, 1481
MnlI CCTC 9 cut(s) 198, 312, 395, 403, 484, 573, 693, 871, 1267
Mox20I TGGCCA 1 cut(s) 1270
Mph1103I ATGCAT 2 cut(s) 155, 999
MroXI GAANNNNTTC 1 cut(s) 63
MscI TGGCCA 1 cut(s) 1270
MseI TTAA 4 cut(s) 69, 905, 939, 1520
Msp20I TGGCCA 1 cut(s) 1270
MspA1I CMGCKG 3 cut(s) 659, 767, 1331
MspI CCGG 3 cut(s) 1082, 1337, 1451
MspR9I CCNGG 3 cut(s) 1083, 1215, 1293
MvaI CCWGG 2 cut(s) 1215, 1293
NciI CCSGG 1 cut(s) 1083
NdeII GATC 2 cut(s) 168, 577
NlaIII CATG 8 cut(s) 157, 228, 461, 584, 721, 1045, 1174, 1201
NlaIV GGNNCC 6 cut(s) 114, 170, 594, 1070, 1335, 1455
NmuCI GTSAC 3 cut(s) 19, 981, 1446
NsiI ATGCAT 2 cut(s) 155, 999
NspI RCATGY 2 cut(s) 157, 721
PaeI GCATGC 2 cut(s) 157, 721
PaqCI CACCTGC 1 cut(s) 229
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 3 cut(s) 546, 873, 893
PflMI CCANNNNNTGG 3 cut(s) 429, 1277, 1412
PfoI TCCNGGA 1 cut(s) 1291
PleI GAGTC 1 cut(s) 963
PpsI GAGTC 1 cut(s) 963
PpuMI RGGWCCY 1 cut(s) 592
Psp124BI GAGCTC 1 cut(s) 33
Psp5II RGGWCCY 1 cut(s) 592
Psp6I CCWGG 2 cut(s) 1213, 1291
PspFI CCCAGC 1 cut(s) 1405
PspGI CCWGG 2 cut(s) 1213, 1291
PspN4I GGNNCC 6 cut(s) 114, 170, 594, 1070, 1335, 1455
PspPI GGNCC 7 cut(s) 113, 123, 572, 592, 1069, 1201, 1334
PspPPI RGGWCCY 1 cut(s) 592
PstI CTGCAG 3 cut(s) 1051, 1183, 1471
PstNI CAGNNNCTG 1 cut(s) 662
PsuI RGATCY 1 cut(s) 168
PvuII CAGCTG 2 cut(s) 659, 767
RsaI GTAC 2 cut(s) 189, 588
RsaNI GTAC 2 cut(s) 188, 587
SacI GAGCTC 1 cut(s) 33
SaqAI TTAA 4 cut(s) 69, 905, 939, 1520
Sau3AI GATC 2 cut(s) 168, 577
Sau96I GGNCC 7 cut(s) 113, 123, 572, 592, 1069, 1201, 1334
SchI GAGTC 1 cut(s) 964
ScrFI CCNGG 3 cut(s) 1083, 1215, 1293
SduI GDGCHC 2 cut(s) 33, 1088
SfaNI GCATC 5 cut(s) 745, 818, 875, 1006, 1293
SfcI CTRYAG 4 cut(s) 1047, 1179, 1308, 1467
SinI GGWCC 5 cut(s) 113, 592, 1069, 1201, 1334
SphI GCATGC 2 cut(s) 157, 721
Sse9I AATT 7 cut(s) 71, 331, 472, 485, 816, 1117, 1287
SsiI CCGC 1 cut(s) 1331
SspMI CTAG 3 cut(s) 399, 516, 1377
SstI GAGCTC 1 cut(s) 33
StyD4I CCNGG 3 cut(s) 1081, 1213, 1291
StyI CCWWGG 4 cut(s) 24, 108, 198, 216
TaaI ACNGT 3 cut(s) 530, 900, 1545
TaiI ACGT 3 cut(s) 21, 409, 1376
TaqI TCGA 1 cut(s) 876
TasI AATT 7 cut(s) 71, 331, 472, 485, 816, 1117, 1287
TatI WGTACW 1 cut(s) 586
TfiI GAWTC 3 cut(s) 546, 873, 893
Tru1I TTAA 4 cut(s) 69, 905, 939, 1520
Tru9I TTAA 4 cut(s) 69, 905, 939, 1520
TscAI CASTG 6 cut(s) 133, 164, 457, 535, 745, 903
TseFI GTSAC 3 cut(s) 19, 981, 1446
Tsp45I GTSAC 3 cut(s) 19, 981, 1446
TspDTI ATGAA 5 cut(s) 17, 282, 366, 819, 1030
TspRI CASTG 6 cut(s) 133, 164, 457, 535, 745, 903
Van91I CCANNNNNTGG 3 cut(s) 429, 1277, 1412
VpaK11BI GGWCC 5 cut(s) 113, 592, 1069, 1201, 1334
XagI CCTNNNNNAGG 2 cut(s) 399, 567
XapI RAATTY 4 cut(s) 331, 472, 485, 1287
XbaI TCTAGA 1 cut(s) 398
XceI RCATGY 2 cut(s) 157, 721
XcmI CCANNNNNNNNNTGG 1 cut(s) 1211
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 3 cut(s) 399, 516, 1377
Zsp2I ATGCAT 2 cut(s) 155, 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.