pycom14g05350

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001537
Physical Location & Seq
Forward (+)
5131547 .. 5132013
467 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g05350.1

Sequence Viewer

Length: 246 bp
ATGTGCAGCTTTAGCAGCAGCTCCCAGTCCGAGCTCAAGTCACCAATGGCGCAAGTTAGCTCCCCCAGGGCCTTCTTCTTCGTGCAGATGCTGGTATTAGCAGTCTTGGCTGCGACGGTCTCGGCTCAGGAGAGCGCGCCGGCGCCGGCACCGATGGACGCTGGAGCGGCGTACTCTTCGCCAGTTAATGGAGTTGTTATTGGAGCATCACTTTTGGTCTCCGTTTTCGTATTTTTCAAGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

82

Amino Acids

8.36

Weight (kDa)

6.51

Isoelectric Point (pI)

58.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 142, 148
AccB7I CCANNNNNTGG 1 cut(s) 188
AccBSI CCGCTC 1 cut(s) 167
AccII CGCG 1 cut(s) 137
AciI CCGC 1 cut(s) 167
AcyI GRCGYC 1 cut(s) 143
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 1 cut(s) 188
AgsI TTSAA 1 cut(s) 238
AjnI CCWGG 1 cut(s) 65
AluBI AGCT 4 cut(s) 9, 21, 34, 60
AluI AGCT 4 cut(s) 9, 21, 34, 60
Alw21I GWGCWC 1 cut(s) 36
Alw26I GTCTC 2 cut(s) 124, 223
AlwNI CAGNNNCTG 1 cut(s) 91
AoxI GGCC 1 cut(s) 69
ApeKI GCWGC 4 cut(s) 6, 15, 18, 110
AspLEI GCGC 4 cut(s) 52, 137, 139, 145
AspS9I GGNCC 1 cut(s) 69
AsuHPI GGTGA 1 cut(s) 33
BanI GGYRCC 2 cut(s) 142, 148
BanII GRGCYC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 36
BbvI GCAGC 4 cut(s) 18, 27, 30, 97
BccI CCATC 1 cut(s) 148
BciT130I CCWGG 1 cut(s) 67
BcoDI GTCTC 2 cut(s) 124, 223
BfaI CTAG 1 cut(s) 244
BfoI RGCGCY 1 cut(s) 146
BisI GCNGC 5 cut(s) 7, 16, 19, 111, 168
BlsI GCNGC 5 cut(s) 8, 17, 20, 112, 169
Bme1390I CCNGG 1 cut(s) 67
BmgT120I GGNCC 1 cut(s) 69
BmiI GGNNCC 2 cut(s) 144, 150
BmrFI CCNGG 1 cut(s) 67
BmrI ACTGGG 1 cut(s) 19
BmsI GCATC 2 cut(s) 78, 215
BmuI ACTGGG 1 cut(s) 19
BpmI CTGGAG 1 cut(s) 183
Bpu10I CCTNAGC 1 cut(s) 126
BpuEI CTTGAG 1 cut(s) 20
BsaHI GRCGYC 1 cut(s) 143
BsaI GGTCTC 2 cut(s) 124, 223
BsaJI CCNNGG 2 cut(s) 65, 66
Bsc4I CCNNNNNNNGG 1 cut(s) 188
Bse118I RCCGGY 2 cut(s) 139, 145
Bse1I ACTGG 2 cut(s) 25, 182
BseBI CCWGG 1 cut(s) 67
BseDI CCNNGG 2 cut(s) 65, 66
BseLI CCNNNNNNNGG 1 cut(s) 188
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 2 cut(s) 25, 182
BsePI GCGCGC 1 cut(s) 135
BseXI GCAGC 4 cut(s) 18, 27, 30, 97
BsgI GTGCAG 2 cut(s) 25, 104
Bsh1236I CGCG 1 cut(s) 137
BshFI GGCC 1 cut(s) 71
BshNI GGYRCC 2 cut(s) 142, 148
BsiHKAI GWGCWC 1 cut(s) 36
BsiSI CCGG 2 cut(s) 140, 146
BslI CCNNNNNNNGG 1 cut(s) 188
BsmAI GTCTC 2 cut(s) 124, 223
BsnI GGCC 1 cut(s) 71
Bso31I GGTCTC 2 cut(s) 124, 223
Bsp1286I GDGCHC 1 cut(s) 36
BspACI CCGC 1 cut(s) 167
BspANI GGCC 1 cut(s) 71
BspCNI CTCAG 1 cut(s) 139
BspFNI CGCG 1 cut(s) 137
BspLI GGNNCC 2 cut(s) 144, 150
BspT107I GGYRCC 2 cut(s) 142, 148
BspTNI GGTCTC 2 cut(s) 124, 223
BsrBI CCGCTC 1 cut(s) 167
BsrFI RCCGGY 2 cut(s) 139, 145
BsrI ACTGG 2 cut(s) 25, 182
BssAI RCCGGY 2 cut(s) 139, 145
BssECI CCNNGG 2 cut(s) 65, 66
BssHII GCGCGC 1 cut(s) 135
BssNI GRCGYC 1 cut(s) 143
Bst2UI CCWGG 1 cut(s) 67
Bst4CI ACNGT 1 cut(s) 118
Bst6I CTCTTC 1 cut(s) 181
BstACI GRCGYC 1 cut(s) 143
BstC8I GCNNGC 3 cut(s) 137, 141, 147
BstDEI CTNAG 1 cut(s) 126
BstFNI CGCG 1 cut(s) 137
BstH2I RGCGCY 1 cut(s) 146
BstHHI GCGC 4 cut(s) 52, 137, 139, 145
BstMAI GTCTC 2 cut(s) 124, 223
BstMWI GCNNNNNNNGC 4 cut(s) 12, 15, 107, 167
BstNI CCWGG 1 cut(s) 67
BstSCI CCNGG 1 cut(s) 65
BstUI CGCG 1 cut(s) 137
BstV1I GCAGC 4 cut(s) 18, 27, 30, 97
BsuRI GGCC 1 cut(s) 71
Cac8I GCNNGC 3 cut(s) 137, 141, 147
CaiI CAGNNNCTG 1 cut(s) 91
CfoI GCGC 4 cut(s) 52, 137, 139, 145
Cfr10I RCCGGY 2 cut(s) 139, 145
Cfr13I GGNCC 1 cut(s) 69
CseI GACGC 1 cut(s) 167
Csp6I GTAC 1 cut(s) 172
CviJI RGCY 7 cut(s) 9, 21, 34, 60, 71, 110, 125
CviKI_1 RGCY 7 cut(s) 9, 21, 34, 60, 71, 110, 125
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 1 cut(s) 126
DinI GGCGCC 1 cut(s) 144
Eam1104I CTCTTC 1 cut(s) 181
EarI CTCTTC 1 cut(s) 181
Ecl136II GAGCTC 1 cut(s) 34
Eco24I GRGCYC 1 cut(s) 36
Eco31I GGTCTC 2 cut(s) 124, 223
Eco53kI GAGCTC 1 cut(s) 34
EcoICRI GAGCTC 1 cut(s) 34
EcoO109I RGGNCCY 1 cut(s) 69
EcoRII CCWGG 1 cut(s) 65
EcoT38I GRGCYC 1 cut(s) 36
EgeI GGCGCC 1 cut(s) 144
EheI GGCGCC 1 cut(s) 144
Fnu4HI GCNGC 5 cut(s) 7, 16, 19, 111, 168
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 5 cut(s) 7, 16, 19, 111, 168
FspBI CTAG 1 cut(s) 244
GlaI GCGC 4 cut(s) 51, 136, 138, 144
GluI GCNGC 5 cut(s) 7, 16, 19, 111, 168
GsuI CTGGAG 1 cut(s) 183
HaeII RGCGCY 1 cut(s) 146
HaeIII GGCC 1 cut(s) 71
HapII CCGG 2 cut(s) 140, 146
HgaI GACGC 1 cut(s) 167
HhaI GCGC 4 cut(s) 52, 137, 139, 145
Hin1I GRCGYC 1 cut(s) 143
Hin6I GCGC 4 cut(s) 50, 135, 137, 143
HinP1I GCGC 4 cut(s) 50, 135, 137, 143
HpaII CCGG 2 cut(s) 140, 146
HphI GGTGA 1 cut(s) 33
Hpy188I TCNGA 1 cut(s) 31
Hpy188III TCNNGA 1 cut(s) 128
Hpy99I CGWCG 1 cut(s) 118
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4V TGCA 2 cut(s) 6, 85
HpyF10VI GCNNNNNNNGC 4 cut(s) 12, 15, 107, 167
HpyF3I CTNAG 1 cut(s) 126
Hsp92I GRCGYC 1 cut(s) 143
HspAI GCGC 4 cut(s) 50, 135, 137, 143
KasI GGCGCC 1 cut(s) 142
KroI GCCGGC 2 cut(s) 139, 145
KroNI GCCGGC 2 cut(s) 141, 147
LmnI GCTCC 4 cut(s) 26, 65, 164, 203
LpnPI CCDG 9 cut(s) 38, 52, 77, 79, 113, 147, 153, 159, 195
Lsp1109I GCAGC 4 cut(s) 18, 27, 30, 97
LweI GCATC 2 cut(s) 78, 215
MaeI CTAG 1 cut(s) 244
MaeIII GTNAC 1 cut(s) 39
MbiI CCGCTC 1 cut(s) 167
MboII GAAGA 3 cut(s) 67, 70, 168
MhlI GDGCHC 1 cut(s) 36
Mly113I GGCGCC 1 cut(s) 143
MreI CGCCGGCG 1 cut(s) 139
MroNI GCCGGC 2 cut(s) 139, 145
MseI TTAA 1 cut(s) 186
MspI CCGG 2 cut(s) 140, 146
MspR9I CCNGG 1 cut(s) 67
MvaI CCWGG 1 cut(s) 67
MvnI CGCG 1 cut(s) 137
MwoI GCNNNNNNNGC 4 cut(s) 12, 15, 107, 167
NaeI GCCGGC 2 cut(s) 141, 147
NarI GGCGCC 1 cut(s) 143
NgoMIV GCCGGC 2 cut(s) 139, 145
NlaIV GGNNCC 2 cut(s) 144, 150
NmeAIII GCCGAG 1 cut(s) 101
NmuCI GTSAC 1 cut(s) 39
PasI CCCWGGG 1 cut(s) 66
PauI GCGCGC 1 cut(s) 135
PdiI GCCGGC 2 cut(s) 141, 147
PflMI CCANNNNNTGG 1 cut(s) 188
PkrI GCNGC 5 cut(s) 8, 17, 20, 112, 169
PluTI GGCGCC 1 cut(s) 146
Psp124BI GAGCTC 1 cut(s) 36
Psp6I CCWGG 1 cut(s) 65
PspGI CCWGG 1 cut(s) 65
PspN4I GGNNCC 2 cut(s) 144, 150
PspPI GGNCC 1 cut(s) 69
PstNI CAGNNNCTG 1 cut(s) 91
PteI GCGCGC 1 cut(s) 135
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
SacI GAGCTC 1 cut(s) 36
SaqAI TTAA 1 cut(s) 186
SatI GCNGC 5 cut(s) 7, 16, 19, 111, 168
Sau96I GGNCC 1 cut(s) 69
ScrFI CCNGG 1 cut(s) 67
SduI GDGCHC 1 cut(s) 36
SetI ASST 4 cut(s) 11, 23, 36, 62
SfaNI GCATC 2 cut(s) 78, 215
SfoI GGCGCC 1 cut(s) 144
SgrAI CRCCGGYG 1 cut(s) 139
SmlI CTYRAG 1 cut(s) 35
SmoI CTYRAG 1 cut(s) 35
SsiI CCGC 1 cut(s) 167
SspDI GGCGCC 1 cut(s) 142
SspMI CTAG 1 cut(s) 244
SstI GAGCTC 1 cut(s) 36
StyD4I CCNGG 1 cut(s) 65
TaaI ACNGT 1 cut(s) 118
TauI GCSGC 1 cut(s) 170
Tru1I TTAA 1 cut(s) 186
Tru9I TTAA 1 cut(s) 186
TseFI GTSAC 1 cut(s) 39
TseI GCWGC 4 cut(s) 6, 15, 18, 110
Tsp45I GTSAC 1 cut(s) 39
TspGWI ACGGA 1 cut(s) 211
Van91I CCANNNNNTGG 1 cut(s) 188
XspI CTAG 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.