Rorug03G0335300

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001309
Physical Location & Seq
Forward (+)
39387565 .. 39389891
2327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0335300.1

Sequence Viewer

Length: 870 bp
ATGGAGGACACGATGCCTATTCGCAAGACTGATACTCTAACTCTGGTTAACATTCAGAGCCTTACTGCAGCTGATCTTGATAGATATGGAGTGAATGTATGGCTTCTGTCTCCTCCTTGCCAACCCTATACTAGACAAGGTCTCCAAAAGCAATCTGGTGATGCTCGGGCATTTTCATTTCTCCAGATTCTTGAGTTGATACCACACACCTCGCAACCTCCAATGATGTTATTTGTGGAAAATGTTGTTGGATTTGAGACATCTGATACACATGGAAAAATGATAGAGATATTGATGAGAAGAAGTGATTTTGTTACACAGGAGTTCATATTGAGTCCCTTGCAGTTTGGTGTACCATATTCCAGGCCACGCTATTTTTGCCTGGCAAAGAGGAAACCGTCAACCTTTCATAGTCAACTTTTCAATAATCAGCTTCTTTGGTCCCCAAAGCCCTTATTTGGGCATACAAATGCGGATGACAAAGTGTTCTGTGAACTTGATGAGCCGCAAGGTAGCTTGGATAAGTTGCTTGAATCTTGTGAGCCAATAGAAATCTTTCTTGAATTCAAGAATTGCCGTGATCAGCTAAACTTTGTGGACACCAATGTTTATACAGATACCATTGGAGTATTGGAGAAAGATGAAGAGAATGGGTGTTGTACTAGCACCTTGGATCAGTACTCTGTTCCCTCAAGCTTGATAGAGAGATGGGGAAGTGCCATGGGTATCCCAAGCTCACTCCTAATTGCATTAGGATTCCTACTTCAACTGGGATTCCTTTTCCTGGTAGGATTAGGAAAGCTTCATTTCTCCATTTCTGATCATTTCTGCGCCTCATTTCCTCCTTGCCTTCATTTTCATCATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

289

Amino Acids

32.83

Weight (kDa)

5.23

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 18 - 138 2.4e-11 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 473, 506
AclWI GGATC 1 cut(s) 681
AcsI RAATTY 1 cut(s) 563
AfaI GTAC 3 cut(s) 354, 661, 680
AfiI CCNNNNNNNGG 3 cut(s) 458, 459, 784
AgsI TTSAA 5 cut(s) 424, 533, 563, 568, 767
AjnI CCWGG 3 cut(s) 362, 381, 783
AjuI GAANNNNNNNTTGG 2 cut(s) 231, 263
AluBI AGCT 7 cut(s) 71, 433, 516, 586, 696, 735, 802
AluI AGCT 7 cut(s) 71, 433, 516, 586, 696, 735, 802
Alw26I GTCTC 3 cut(s) 114, 146, 251
AlwI GGATC 1 cut(s) 681
Ama87I CYCGRG 1 cut(s) 165
AoxI GGCC 1 cut(s) 365
ApeKI GCWGC 1 cut(s) 68
ApoI RAATTY 1 cut(s) 563
Asp700I GAANNNNTTC 1 cut(s) 555
AspLEI GCGC 1 cut(s) 833
AspS9I GGNCC 1 cut(s) 441
AsuHPI GGTGA 1 cut(s) 170
AvaI CYCGRG 1 cut(s) 165
AvaII GGWCC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 80
BccI CCATC 1 cut(s) 702
BceAI ACGGC 1 cut(s) 561
BciT130I CCWGG 3 cut(s) 364, 383, 785
BciVI GTATCC 1 cut(s) 737
BclI TGATCA 2 cut(s) 580, 820
BcoDI GTCTC 3 cut(s) 114, 146, 251
BfaI CTAG 3 cut(s) 132, 663, 868
BfmI CTRYAG 1 cut(s) 66
BfuI GTATCC 1 cut(s) 737
BisI GCNGC 2 cut(s) 69, 506
BlsI GCNGC 2 cut(s) 70, 507
BmcAI AGTACT 1 cut(s) 680
Bme1390I CCNGG 3 cut(s) 364, 383, 785
Bme18I GGWCC 1 cut(s) 441
BmeT110I CYCGRG 1 cut(s) 165
BmgT120I GGNCC 1 cut(s) 441
BmiI GGNNCC 1 cut(s) 443
BmrFI CCNGG 3 cut(s) 364, 383, 785
BmrI ACTGGG 1 cut(s) 779
BmsI GCATC 2 cut(s) 3, 151
BmuI ACTGGG 1 cut(s) 779
BpmI CTGGAG 1 cut(s) 167
BpuEI CTTGAG 2 cut(s) 212, 676
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 2 cut(s) 669, 720
BsaXI ACNNNNNCTCC 4 cut(s) 81, 111, 126, 156
Bsc4I CCNNNNNNNGG 3 cut(s) 458, 459, 784
Bse1I ACTGG 1 cut(s) 774
BseBI CCWGG 3 cut(s) 364, 383, 785
BseDI CCNNGG 2 cut(s) 669, 720
BseGI GGATG 1 cut(s) 481
BseLI CCNNNNNNNGG 3 cut(s) 458, 459, 784
BseNI ACTGG 1 cut(s) 774
BseRI GAGGAG 1 cut(s) 102
BseXI GCAGC 1 cut(s) 80
BshFI GGCC 1 cut(s) 367
BsiHKCI CYCGRG 1 cut(s) 165
BslFI GGGAC 2 cut(s) 321, 427
BslI CCNNNNNNNGG 3 cut(s) 458, 459, 784
BsmAI GTCTC 3 cut(s) 114, 146, 251
BsmFI GGGAC 2 cut(s) 321, 427
BsnI GGCC 1 cut(s) 367
Bso31I GGTCTC 1 cut(s) 146
BsoBI CYCGRG 1 cut(s) 165
Bsp143I GATC 4 cut(s) 73, 580, 673, 820
Bsp19I CCATGG 1 cut(s) 720
BspACI CCGC 2 cut(s) 473, 506
BspANI GGCC 1 cut(s) 367
BspLI GGNNCC 1 cut(s) 443
BspMAI CTGCAG 1 cut(s) 70
BspPI GGATC 1 cut(s) 681
BspTNI GGTCTC 1 cut(s) 146
BsrI ACTGG 1 cut(s) 774
BssECI CCNNGG 2 cut(s) 669, 720
BssMI GATC 4 cut(s) 73, 580, 673, 820
BssT1I CCWWGG 2 cut(s) 669, 720
Bst2UI CCWGG 3 cut(s) 364, 383, 785
Bst4CI ACNGT 1 cut(s) 399
Bst6I CTCTTC 1 cut(s) 639
BstDSI CCRYGG 1 cut(s) 720
BstF5I GGATG 1 cut(s) 481
BstHHI GCGC 1 cut(s) 833
BstKTI GATC 4 cut(s) 76, 583, 676, 823
BstMAI GTCTC 3 cut(s) 114, 146, 251
BstMBI GATC 4 cut(s) 73, 580, 673, 820
BstMWI GCNNNNNNNGC 1 cut(s) 378
BstNI CCWGG 3 cut(s) 364, 383, 785
BstSCI CCNGG 3 cut(s) 362, 381, 783
BstSFI CTRYAG 1 cut(s) 66
BstV1I GCAGC 1 cut(s) 80
BsuI GTATCC 1 cut(s) 737
BsuRI GGCC 1 cut(s) 367
BtgI CCRYGG 1 cut(s) 720
BtsCI GGATG 1 cut(s) 481
CfoI GCGC 1 cut(s) 833
Cfr13I GGNCC 1 cut(s) 441
Csp6I GTAC 3 cut(s) 353, 660, 679
CviAII CATG 2 cut(s) 272, 721
CviQI GTAC 3 cut(s) 353, 660, 679
DpnI GATC 4 cut(s) 75, 582, 675, 822
DpnII GATC 4 cut(s) 73, 580, 673, 820
Eam1104I CTCTTC 1 cut(s) 639
EarI CTCTTC 1 cut(s) 639
Eco130I CCWWGG 2 cut(s) 669, 720
Eco31I GGTCTC 1 cut(s) 146
Eco47I GGWCC 1 cut(s) 441
Eco88I CYCGRG 1 cut(s) 165
EcoRI GAATTC 1 cut(s) 563
EcoRII CCWGG 3 cut(s) 362, 381, 783
EcoT14I CCWWGG 2 cut(s) 669, 720
ErhI CCWWGG 2 cut(s) 669, 720
FaeI CATG 2 cut(s) 275, 724
FaqI GGGAC 2 cut(s) 321, 427
FatI CATG 2 cut(s) 271, 720
FbaI TGATCA 2 cut(s) 580, 820
Fnu4HI GCNGC 2 cut(s) 69, 506
FokI GGATG 1 cut(s) 488
Fsp4HI GCNGC 2 cut(s) 69, 506
FspBI CTAG 3 cut(s) 132, 663, 868
GlaI GCGC 1 cut(s) 832
GluI GCNGC 2 cut(s) 69, 506
GsuI CTGGAG 1 cut(s) 167
HaeIII GGCC 1 cut(s) 367
HhaI GCGC 1 cut(s) 833
Hin1II CATG 2 cut(s) 275, 724
Hin6I GCGC 1 cut(s) 831
HinP1I GCGC 1 cut(s) 831
HincII GTYRAC 3 cut(s) 49, 402, 416
HindII GTYRAC 3 cut(s) 49, 402, 416
HindIII AAGCTT 2 cut(s) 694, 800
HinfI GANTC 5 cut(s) 187, 334, 533, 756, 774
HpaI GTTAAC 1 cut(s) 49
HphI GGTGA 1 cut(s) 170
Hpy166II GTNNAC 6 cut(s) 49, 353, 402, 416, 494, 598
Hpy188I TCNGA 3 cut(s) 57, 265, 820
Hpy188III TCNNGA 5 cut(s) 77, 184, 191, 560, 568
Hpy8I GTNNAC 6 cut(s) 49, 353, 402, 416, 494, 598
HpyAV CCTTC 1 cut(s) 860
HpyCH4III ACNGT 1 cut(s) 399
HpyCH4V TGCA 3 cut(s) 68, 343, 749
HpyF10VI GCNNNNNNNGC 1 cut(s) 378
Hsp92II CATG 2 cut(s) 275, 724
HspAI GCGC 1 cut(s) 831
Ksp22I TGATCA 2 cut(s) 580, 820
KspAI GTTAAC 1 cut(s) 49
Kzo9I GATC 4 cut(s) 73, 580, 673, 820
Lsp1109I GCAGC 1 cut(s) 80
LweI GCATC 2 cut(s) 3, 151
MaeI CTAG 3 cut(s) 132, 663, 868
MaeIII GTNAC 1 cut(s) 313
MalI GATC 4 cut(s) 75, 582, 675, 822
MboI GATC 4 cut(s) 73, 580, 673, 820
MboII GAAGA 2 cut(s) 312, 656
MluCI AATT 3 cut(s) 563, 571, 744
MlyI GAGTC 1 cut(s) 343
MmeI TCCRAC 1 cut(s) 229
MnlI CCTC 7 cut(s) 123, 220, 228, 384, 700, 844, 852
MroXI GAANNNNTTC 1 cut(s) 555
MseI TTAA 1 cut(s) 48
MslI CAYNNNNRTG 1 cut(s) 468
MspA1I CMGCKG 1 cut(s) 71
MspR9I CCNGG 3 cut(s) 364, 383, 785
MvaI CCWGG 3 cut(s) 364, 383, 785
MwoI GCNNNNNNNGC 1 cut(s) 378
NcoI CCATGG 1 cut(s) 720
NdeII GATC 4 cut(s) 73, 580, 673, 820
NlaIII CATG 2 cut(s) 275, 724
NlaIV GGNNCC 1 cut(s) 443
PdmI GAANNNNTTC 1 cut(s) 555
PfeI GAWTC 4 cut(s) 187, 533, 756, 774
PflFI GACNNNGTC 1 cut(s) 138
PkrI GCNGC 2 cut(s) 70, 507
PleI GAGTC 1 cut(s) 342
PpsI GAGTC 1 cut(s) 342
Psp6I CCWGG 3 cut(s) 362, 381, 783
PspGI CCWGG 3 cut(s) 362, 381, 783
PspN4I GGNNCC 1 cut(s) 443
PspPI GGNCC 1 cut(s) 441
PstI CTGCAG 1 cut(s) 70
PsyI GACNNNGTC 1 cut(s) 138
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 3 cut(s) 354, 661, 680
RsaNI GTAC 3 cut(s) 353, 660, 679
RseI CAYNNNNRTG 1 cut(s) 468
SaqAI TTAA 1 cut(s) 48
SatI GCNGC 2 cut(s) 69, 506
Sau3AI GATC 4 cut(s) 73, 580, 673, 820
Sau96I GGNCC 1 cut(s) 441
ScaI AGTACT 1 cut(s) 680
SchI GAGTC 1 cut(s) 343
ScrFI CCNGG 3 cut(s) 364, 383, 785
SfaNI GCATC 2 cut(s) 3, 151
SfcI CTRYAG 1 cut(s) 66
SinI GGWCC 1 cut(s) 441
SmiMI CAYNNNNRTG 1 cut(s) 468
SmlI CTYRAG 2 cut(s) 191, 691
SmoI CTYRAG 2 cut(s) 191, 691
Sse9I AATT 3 cut(s) 563, 571, 744
SsiI CCGC 2 cut(s) 473, 506
SspMI CTAG 3 cut(s) 132, 663, 868
StyD4I CCNGG 3 cut(s) 362, 381, 783
StyI CCWWGG 2 cut(s) 669, 720
TaaI ACNGT 1 cut(s) 399
TasI AATT 3 cut(s) 563, 571, 744
TatI WGTACW 2 cut(s) 659, 678
TauI GCSGC 1 cut(s) 508
TfiI GAWTC 4 cut(s) 187, 533, 756, 774
Tru1I TTAA 1 cut(s) 48
Tru9I TTAA 1 cut(s) 48
TseI GCWGC 1 cut(s) 68
TspDTI ATGAA 7 cut(s) 165, 316, 398, 657, 794, 842, 848
Tth111I GACNNNGTC 1 cut(s) 138
VpaK11BI GGWCC 1 cut(s) 441
XapI RAATTY 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 2 cut(s) 152, 628
XmnI GAANNNNTTC 1 cut(s) 555
XspI CTAG 3 cut(s) 132, 663, 868
ZrmI AGTACT 1 cut(s) 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.