Prupe.6G300700_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001309
Physical Location & Seq
Forward (+)
27293230 .. 27297748
4519 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G300700.1

Sequence Viewer

Length: 1326 bp
ATGGCTTCCACCGAGGGTATAGTACCAATAACCAGGGCTTTTCTGGCTTCCTATTACGACACATACCAATTCTCCCCTCTCTCAAACGACGTCGCTCGCCTTTCCTCTGAGATTCGCTCTTTCACCGCCGATTTGCTTAACAGCTCTCCGGCTGCTGAAGGCGAGGAGAGATTATTAGTGAGTGAGTTAGAGAGTGAACCGCCCCATAAAGTTGACGAGAACATGTGGAAGAATCGAGAACACATGGAGGAGATACTGTATTTACTTGAAAAGCCTCATTGGCCTCGATCGCTTCAACAGCAGTTAAGTCCTGATGATGCTGAACTTGCTACTATTATCGATCGTCTCCACCAAAAGTTTCAAACCAGTTTGAAGACATTGGAGTCTTTCCAAGCTAAAAATTCTGAACGTGTCTTCAACACAGTTATGACTTACATTCCCCAAGATTTTCGGGGAAAGCTCATCAGACAACAGAGGGAACGTTCAGAGAGGAATAAGCAAGCAGAGGTTGATGCTTTGGTTAGTTCTGGAGCATCTATACGTGATCGTTATGCTCTCTTGTGGAAACAACAGATGGAGAGGAGGAGACTTTTAGCTCAGCTTGGTTCTGCTACTGGTGTCTACAAAACCCTTGTCAAGTACTTGGTTGGGGTTCCACAGGTTCTACTAGATTTTATTCGCCAAATAAATGATGATGATGGGCCCATGGAAGAACAACGACAACGTTATGGACCACCTTTATATAGACTTACAACTATGGTGCTTCTTATAAGAGTCTTTATTTCACTGTCTTGGGGGCGTTTTGAGACTAGAAAATTAAAGAAAGAAGAGGTAGCTGTATTGGAACAAGCTGCTGATGTCTACACCACTGAGTTTGAAAGGTTTATAACGTTTATCAGCGAGGTCTTTGCAAATTCCCCCTTTTTTATCTCAGCAGAGGTTGCCGGTGCATTAGAAGGAAGGAATAATGATGATTACAAAGAGATAAGTGTTCCGGCTGGGAAAACTCATGAGGTTTCATTGTCTGTGGACGCGATAAATTCATACATTGCTTGGGATTTTTCGCTTGTGCAAGGGAAGATAAACTTGGATATTGGATTTAGTGTCGAGTATACAAATCCTTCTGGAGAAAAATCTCTCATTTTACCTTACCGACGCTTCGAGTCTGACCAAGGAAACTTCTGCACATGCGAGGTTGGAAACTATAAACTGCTTTGGGACAATTCATATTCAAGTTTCTTTAAGAAGGCGCTGCGCTACAAGGTAGATTGCATACCTCCAGTTGCTGAGCCAGTGGCACCCGCCGCAACTGAAGCTAAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

442

Amino Acids

50.53

Weight (kDa)

5.34

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 770, 887
AasI GACNNNNNNGTC 1 cut(s) 382
AatII GACGTC 1 cut(s) 93
AccB1I GGYRCC 1 cut(s) 1297
AccI GTMKAC 3 cut(s) 621, 861, 1112
AccII CGCG 1 cut(s) 1034
AciI CCGC 4 cut(s) 126, 200, 1302, 1305
AclI AACGTT 3 cut(s) 481, 724, 890
AcsI RAATTY 3 cut(s) 400, 913, 1039
AcuI CTGAAG 1 cut(s) 177
AcyI GRCGYC 1 cut(s) 90
AfaI GTAC 2 cut(s) 24, 641
AflIII ACRYGT 2 cut(s) 222, 409
AgsI TTSAA 7 cut(s) 269, 296, 362, 373, 418, 878, 1233
AjnI CCWGG 1 cut(s) 32
AjuI GAANNNNNNNTTGG 2 cut(s) 1070, 1102
AluBI AGCT 8 cut(s) 144, 395, 460, 596, 601, 836, 851, 1316
AluI AGCT 8 cut(s) 144, 395, 460, 596, 601, 836, 851, 1316
Alw26I GTCTC 3 cut(s) 350, 580, 800
AlwNI CAGNNNCTG 1 cut(s) 1286
AoxI GGCC 2 cut(s) 281, 701
ApaI GGGCCC 1 cut(s) 705
ApeKI GCWGC 3 cut(s) 152, 851, 1252
ApoI RAATTY 3 cut(s) 400, 913, 1039
ArsI GACNNNNNNTTYG 2 cut(s) 618, 650
AspLEI GCGC 2 cut(s) 1252, 1257
AspS9I GGNCC 3 cut(s) 701, 702, 731
AsuHPI GGTGA 1 cut(s) 115
AvaII GGWCC 1 cut(s) 731
BaeGI GKGCMC 1 cut(s) 705
BanI GGYRCC 1 cut(s) 1297
BanII GRGCYC 1 cut(s) 705
BbsI GAAGAC 2 cut(s) 380, 406
BbvI GCAGC 3 cut(s) 139, 838, 1239
BccI CCATC 2 cut(s) 568, 692
BcgI CGANNNNNNTGC 2 cut(s) 890, 924
BciT130I CCWGG 1 cut(s) 34
BcoDI GTCTC 3 cut(s) 350, 580, 800
BfaI CTAG 2 cut(s) 668, 810
BfoI RGCGCY 1 cut(s) 1253
BglI GCCNNNNNGGC 1 cut(s) 280
BisI GCNGC 4 cut(s) 153, 852, 1253, 1305
BlpI GCTNAGC 2 cut(s) 597, 1287
BlsI GCNGC 4 cut(s) 154, 853, 1254, 1306
BmcAI AGTACT 1 cut(s) 641
Bme1390I CCNGG 1 cut(s) 34
Bme18I GGWCC 1 cut(s) 731
BmgT120I GGNCC 3 cut(s) 701, 702, 731
BmiI GGNNCC 3 cut(s) 654, 703, 1299
BmrFI CCNGG 1 cut(s) 34
BmsI GCATC 3 cut(s) 307, 502, 542
BpiI GAAGAC 2 cut(s) 380, 406
BplI GAGNNNNNCTC 2 cut(s) 101, 133
BpmI CTGGAG 3 cut(s) 549, 1146, 1263
Bpu1102I GCTNAGC 2 cut(s) 597, 1287
Bsa29I ATCGAT 1 cut(s) 339
BsaAI YACGTR 1 cut(s) 542
BsaHI GRCGYC 1 cut(s) 90
BsaJI CCNNGG 4 cut(s) 12, 33, 705, 1171
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 242, 272
Bse118I RCCGGY 1 cut(s) 944
Bse1I ACTGG 4 cut(s) 366, 619, 1280, 1292
Bse3DI GCAATG 1 cut(s) 1047
BseBI CCWGG 1 cut(s) 34
BseCI ATCGAT 1 cut(s) 339
BseDI CCNNGG 4 cut(s) 12, 33, 705, 1171
BseMI GCAATG 1 cut(s) 1047
BseMII CTCAG 5 cut(s) 99, 611, 861, 945, 1278
BseNI ACTGG 4 cut(s) 366, 619, 1280, 1292
BseRI GAGGAG 4 cut(s) 179, 263, 595, 598
BseSI GKGCMC 1 cut(s) 705
BseXI GCAGC 3 cut(s) 139, 838, 1239
BseYI CCCAGC 1 cut(s) 998
BsgI GTGCAG 1 cut(s) 1168
Bsh1236I CGCG 1 cut(s) 1034
Bsh1285I CGRYCG 2 cut(s) 290, 343
BshFI GGCC 2 cut(s) 283, 703
BshNI GGYRCC 1 cut(s) 1297
BshVI ATCGAT 1 cut(s) 339
BsiEI CGRYCG 2 cut(s) 290, 343
BsiSI CCGG 3 cut(s) 149, 945, 995
BslFI GGGAC 1 cut(s) 1232
BsmAI GTCTC 3 cut(s) 350, 580, 800
BsmBI CGTCTC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 1232
BsnI GGCC 2 cut(s) 283, 703
Bsp120I GGGCCC 1 cut(s) 701
Bsp1286I GDGCHC 1 cut(s) 705
Bsp143I GATC 3 cut(s) 287, 340, 544
Bsp1720I GCTNAGC 2 cut(s) 597, 1287
Bsp19I CCATGG 1 cut(s) 705
BspACI CCGC 4 cut(s) 126, 200, 1302, 1305
BspANI GGCC 2 cut(s) 283, 703
BspCNI CTCAG 5 cut(s) 100, 610, 862, 944, 1279
BspDI ATCGAT 1 cut(s) 339
BspFNI CGCG 1 cut(s) 1034
BspHI TCATGA 1 cut(s) 1009
BspLI GGNNCC 3 cut(s) 654, 703, 1299
BspT107I GGYRCC 1 cut(s) 1297
BsrDI GCAATG 1 cut(s) 1047
BsrFI RCCGGY 1 cut(s) 944
BsrI ACTGG 4 cut(s) 366, 619, 1280, 1292
BssAI RCCGGY 1 cut(s) 944
BssECI CCNNGG 4 cut(s) 12, 33, 705, 1171
BssMI GATC 3 cut(s) 287, 340, 544
BssNAI GTATAC 1 cut(s) 1113
BssNI GRCGYC 1 cut(s) 90
BssT1I CCWWGG 2 cut(s) 705, 1171
Bst1107I GTATAC 1 cut(s) 1113
Bst2UI CCWGG 1 cut(s) 34
Bst4CI ACNGT 3 cut(s) 258, 424, 789
Bst6I CTCTTC 1 cut(s) 822
BstACI GRCGYC 1 cut(s) 90
BstAPI GCANNNNNTGC 1 cut(s) 941
BstBAI YACGTR 1 cut(s) 542
BstC8I GCNNGC 2 cut(s) 97, 501
BstDEI CTNAG 5 cut(s) 108, 597, 870, 931, 1287
BstDSI CCRYGG 1 cut(s) 705
BstFNI CGCG 1 cut(s) 1034
BstH2I RGCGCY 1 cut(s) 1253
BstHHI GCGC 2 cut(s) 1252, 1257
BstKTI GATC 3 cut(s) 290, 343, 547
BstMAI GTCTC 3 cut(s) 350, 580, 800
BstMBI GATC 3 cut(s) 287, 340, 544
BstMCI CGRYCG 2 cut(s) 290, 343
BstMWI GCNNNNNNNGC 8 cut(s) 44, 280, 289, 298, 326, 941, 1304, 1313
BstNI CCWGG 1 cut(s) 34
BstNSI RCATGY 2 cut(s) 226, 1191
BstSCI CCNGG 1 cut(s) 32
BstSLI GKGCMC 1 cut(s) 705
BstUI CGCG 1 cut(s) 1034
BstV1I GCAGC 3 cut(s) 139, 838, 1239
BstV2I GAAGAC 2 cut(s) 380, 406
BstZ17I GTATAC 1 cut(s) 1113
Bsu15I ATCGAT 1 cut(s) 339
BsuRI GGCC 2 cut(s) 283, 703
BsuTUI ATCGAT 1 cut(s) 339
BtgI CCRYGG 1 cut(s) 705
BtsIMutI CAGTG 3 cut(s) 785, 867, 1299
Cac8I GCNNGC 2 cut(s) 97, 501
CaiI CAGNNNCTG 1 cut(s) 1286
CciI TCATGA 1 cut(s) 1009
CfoI GCGC 2 cut(s) 1252, 1257
Cfr10I RCCGGY 1 cut(s) 944
Cfr13I GGNCC 3 cut(s) 701, 702, 731
ClaI ATCGAT 1 cut(s) 339
CseI GACGC 2 cut(s) 1040, 1164
Csp6I GTAC 2 cut(s) 23, 640
CspCI CAANNNNNGTGG 2 cut(s) 856, 891
CviAII CATG 5 cut(s) 223, 244, 706, 1010, 1188
CviQI GTAC 2 cut(s) 23, 640
DdeI CTNAG 5 cut(s) 108, 597, 870, 931, 1287
DpnI GATC 3 cut(s) 289, 342, 546
DpnII GATC 3 cut(s) 287, 340, 544
DrdI GACNNNNNNGTC 1 cut(s) 382
DseDI GACNNNNNNGTC 1 cut(s) 382
Eam1104I CTCTTC 1 cut(s) 822
EarI CTCTTC 1 cut(s) 822
Eco130I CCWWGG 2 cut(s) 705, 1171
Eco24I GRGCYC 1 cut(s) 705
Eco47I GGWCC 1 cut(s) 731
Eco57I CTGAAG 1 cut(s) 177
EcoRII CCWGG 1 cut(s) 32
EcoT14I CCWWGG 2 cut(s) 705, 1171
EcoT38I GRGCYC 1 cut(s) 705
ErhI CCWWGG 2 cut(s) 705, 1171
Esp3I CGTCTC 1 cut(s) 350
FaeI CATG 5 cut(s) 226, 247, 709, 1013, 1191
FalI AAGNNNNNCTT 2 cut(s) 1070, 1102
FaqI GGGAC 1 cut(s) 1232
FatI CATG 5 cut(s) 222, 243, 705, 1009, 1187
FauI CCCGC 1 cut(s) 1309
FblI GTMKAC 3 cut(s) 621, 861, 1112
Fnu4HI GCNGC 4 cut(s) 153, 852, 1253, 1305
FriOI GRGCYC 1 cut(s) 705
Fsp4HI GCNGC 4 cut(s) 153, 852, 1253, 1305
FspBI CTAG 2 cut(s) 668, 810
GlaI GCGC 2 cut(s) 1251, 1256
GluI GCNGC 4 cut(s) 153, 852, 1253, 1305
GsaI CCCAGC 1 cut(s) 1002
GsuI CTGGAG 3 cut(s) 549, 1146, 1263
HaeII RGCGCY 1 cut(s) 1253
HaeIII GGCC 2 cut(s) 283, 703
HapII CCGG 3 cut(s) 149, 945, 995
HgaI GACGC 2 cut(s) 1040, 1164
HhaI GCGC 2 cut(s) 1252, 1257
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 5 cut(s) 226, 247, 709, 1013, 1191
Hin6I GCGC 2 cut(s) 1250, 1255
HinP1I GCGC 2 cut(s) 1250, 1255
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 5 cut(s) 112, 232, 383, 774, 1163
HpaII CCGG 3 cut(s) 149, 945, 995
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 6 cut(s) 197, 214, 622, 862, 1030, 1113
Hpy188I TCNGA 5 cut(s) 109, 406, 467, 487, 1168
Hpy188III TCNNGA 5 cut(s) 236, 311, 528, 1010, 1125
Hpy8I GTNNAC 6 cut(s) 197, 214, 622, 862, 1030, 1113
Hpy99I CGWCG 3 cut(s) 92, 95, 1158
HpyAV CCTTC 5 cut(s) 152, 950, 954, 1131, 1240
HpyCH4III ACNGT 3 cut(s) 258, 424, 789
HpyCH4IV ACGT 6 cut(s) 90, 409, 481, 541, 724, 890
HpyCH4V TGCA 5 cut(s) 911, 950, 1072, 1185, 1272
HpyF10VI GCNNNNNNNGC 8 cut(s) 44, 280, 289, 298, 326, 941, 1304, 1313
HpyF3I CTNAG 5 cut(s) 108, 597, 870, 931, 1287
HpySE526I ACGT 6 cut(s) 90, 409, 481, 541, 724, 890
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 5 cut(s) 226, 247, 709, 1013, 1191
HspAI GCGC 2 cut(s) 1250, 1255
Kzo9I GATC 3 cut(s) 287, 340, 544
LmnI GCTCC 1 cut(s) 530
Lsp1109I GCAGC 3 cut(s) 139, 838, 1239
LweI GCATC 3 cut(s) 307, 502, 542
MaeI CTAG 2 cut(s) 668, 810
MaeII ACGT 6 cut(s) 90, 409, 481, 541, 724, 890
MalI GATC 3 cut(s) 289, 342, 546
MboI GATC 3 cut(s) 287, 340, 544
MboII GAAGA 6 cut(s) 241, 385, 406, 722, 839, 1090
MhlI GDGCHC 1 cut(s) 705
MluCI AATT 6 cut(s) 68, 400, 815, 913, 1039, 1222
MlyI GAGTC 3 cut(s) 392, 783, 1172
MmeI TCCRAC 1 cut(s) 1177
MseI TTAA 4 cut(s) 138, 305, 818, 1242
MslI CAYNNNNRTG 1 cut(s) 425
MspI CCGG 3 cut(s) 149, 945, 995
MspR9I CCNGG 1 cut(s) 34
MteI GCGCNGCGC 1 cut(s) 1253
MvaI CCWGG 1 cut(s) 34
MvnI CGCG 1 cut(s) 1034
MwoI GCNNNNNNNGC 8 cut(s) 44, 280, 289, 298, 326, 941, 1304, 1313
NcoI CCATGG 1 cut(s) 705
NdeII GATC 3 cut(s) 287, 340, 544
NlaIII CATG 5 cut(s) 226, 247, 709, 1013, 1191
NlaIV GGNNCC 3 cut(s) 654, 703, 1299
NspI RCATGY 2 cut(s) 226, 1191
PagI TCATGA 1 cut(s) 1009
PciI ACATGT 1 cut(s) 222
PfeI GAWTC 2 cut(s) 112, 232
PkrI GCNGC 4 cut(s) 154, 853, 1254, 1306
Ple19I CGATCG 2 cut(s) 290, 343
PleI GAGTC 3 cut(s) 391, 782, 1171
PpsI GAGTC 3 cut(s) 391, 782, 1171
Ppu21I YACGTR 1 cut(s) 542
PscI ACATGT 1 cut(s) 222
PsiI TTATAA 2 cut(s) 770, 887
Psp1406I AACGTT 3 cut(s) 481, 724, 890
Psp6I CCWGG 1 cut(s) 32
PspFI CCCAGC 1 cut(s) 998
PspGI CCWGG 1 cut(s) 32
PspN4I GGNNCC 3 cut(s) 654, 703, 1299
PspOMI GGGCCC 1 cut(s) 701
PspPI GGNCC 3 cut(s) 701, 702, 731
PstNI CAGNNNCTG 1 cut(s) 1286
PvuI CGATCG 2 cut(s) 290, 343
RsaI GTAC 2 cut(s) 24, 641
RsaNI GTAC 2 cut(s) 23, 640
RseI CAYNNNNRTG 1 cut(s) 425
SaqAI TTAA 4 cut(s) 138, 305, 818, 1242
SatI GCNGC 4 cut(s) 153, 852, 1253, 1305
Sau3AI GATC 3 cut(s) 287, 340, 544
Sau96I GGNCC 3 cut(s) 701, 702, 731
ScaI AGTACT 1 cut(s) 641
SchI GAGTC 3 cut(s) 392, 783, 1172
ScrFI CCNGG 1 cut(s) 34
SduI GDGCHC 1 cut(s) 705
SfaNI GCATC 3 cut(s) 307, 502, 542
SinI GGWCC 1 cut(s) 731
SmiMI CAYNNNNRTG 1 cut(s) 425
Sse9I AATT 6 cut(s) 68, 400, 815, 913, 1039, 1222
SsiI CCGC 4 cut(s) 126, 200, 1302, 1305
SspMI CTAG 2 cut(s) 668, 810
StyD4I CCNGG 1 cut(s) 32
StyI CCWWGG 2 cut(s) 705, 1171
TaaI ACNGT 3 cut(s) 258, 424, 789
TaiI ACGT 6 cut(s) 93, 412, 484, 544, 727, 893
TaqI TCGA 5 cut(s) 235, 286, 339, 1107, 1161
TasI AATT 6 cut(s) 68, 400, 815, 913, 1039, 1222
TatI WGTACW 1 cut(s) 639
TauI GCSGC 1 cut(s) 1307
TfiI GAWTC 2 cut(s) 112, 232
Tru1I TTAA 4 cut(s) 138, 305, 818, 1242
Tru9I TTAA 4 cut(s) 138, 305, 818, 1242
TscAI CASTG 3 cut(s) 792, 874, 1299
TseI GCWGC 3 cut(s) 152, 851, 1252
TspDTI ATGAA 3 cut(s) 1008, 1032, 1215
TspRI CASTG 3 cut(s) 792, 874, 1299
VpaK11BI GGWCC 1 cut(s) 731
XapI RAATTY 3 cut(s) 400, 913, 1039
XceI RCATGY 2 cut(s) 226, 1191
XcmI CCANNNNNNNNNTGG 1 cut(s) 40
XmiI GTMKAC 3 cut(s) 621, 861, 1112
XspI CTAG 2 cut(s) 668, 810
ZraI GACGTC 1 cut(s) 91
ZrmI AGTACT 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.