Rorug03G0185700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001309
Physical Location & Seq
Forward (+)
16083708 .. 16086661
2954 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0185700.1

Sequence Viewer

Length: 2178 bp
ATGCATATGATCGTAACCACACCCGCCGGCGACTCCGCCTTCACCAACCACGCCTACTGCTCACCGTCCGATCTTCCCACCTTCGCCGTCCCTCAATCCGACCGCTGTTATGCCACCGTCGGCGACAACTACGTTCTATCTATCAGCCCCCAAGAGGGTATAGAGAGCGGCCACATTGCTCTGAATGCGACCCAACAAAAGCAAGCTGGTGTTTCTGTTGGTGACCAAGTTTCTGTCACCAGATTTATTCCGCCCGAGGAGTGTTTTAACTTGGCACTGCTCAAGATAGAGATAGAGTTGCTGGGGAAAAGAACCAAAACTCGACAGGTCGATGCTGTTTTCCTCGACGAGTTACTTAGAAACAAGTTCATGTACCAGGTTATGATGGCTGGACAAAAAGTATCATTCAAGTCTGATGGAGGTCGTGAATACGTTTTCACAGTCCATCAAGCTGCAGTAGAGGGGCAAGAAAAGGCTGATGCTGATCTTGAAAGAGGGATGATTTCATCAGGGACATACTTCATCTTCGAGACACCAAAGGGTTCAAATGGACTAAAGATACTAAATCAACGTAAAATCTTCACGCAGAAGGAACTTAATCTTCAAAAACTTGGCATTGGTGGATTAAATGCAGAGTTTGAAAGTATATTTCGAAGAGCTTTTGCCTCCCGTGTACTCCCTCATCATGTGGTGAATAAATTAGGGATTAAGCATGTGAAGGGCATGCTGCTTTATGGACCGCCCGGTACTGGAAAAACTCTGGTGGCTCGGCAAATAGGGAAAATTTTGAATGGGAAAGATCCAAAGATCGTCAATGGCCCTGAAGTGTTGGACTGTTACGTTGGTGAAAGTGAGAAGAATGTTAGGGAGCTATTTGCTGATGCTGAACAAGATCAAATAGTTCATGGGGATGAAAGTGATTTGCATGTCATAATATTTGATGAAATTGATGCAATTTGTAAGAAAAGATCAGGTTCCAGCCGTGATAGTTCACGCATTCATGATAATATTGTGAACACATTACTTACCAAGATGGATGGGGTGGAGCCTCTCAATAATATTCTGATTATTGGATTGACTAACAGAAAGGAGTTGCTGGATGAAGCCCTTTTGAGACCAGGACGTCTGGAAGTTCAAATTCAGATAGGCCTTCCTGATAAAAAAGGTCGCCTACAGATTCTTCAAATTCATACAAACAAGATAAAGGAGAGCTCTTATCTTGCTCCTGATGTGAACCTTCGAGAGCTTGCTACTCATGCAGAAAACTATAGCGGTGCAGAACTTGAAGGTGTTGTTAGAAGTGCTGTGTCATTTGCTTTTAATAGACAACTGAGTCTGGACGATCTTAAGAAGCCAGTGGACGAGGAGAATATTAAAGTTACTATGGATGATTTTCGTCGCGCACTTCAAGAAATTAGACCTACTTATGGAGCCTTGGCAGAGGAGCTTGAACGATGCAGGTTAAATGGCATTGTGAATTGTGGTGATCGACATAGGCACGCTTATGACAGAGCTATGCTTCTTGTGGAGCAAGTTAAATCAAGTGAAGGAAGTCCTCTTGTCAAATGCCTTCTAGAGGGCCCTAGTGGCACTGGCAAATCTGCCTTAGCAGCTACCATTGGCATCCACAGCCGATTTCCATTTGTCAGGATTGTTTCGGCTGAGTCCATGATTGGTCTTCATGACGAGAGGGCAAAGTGTGTTCACCTTGAAAACGTCTTCGAGGAAGCTTACAAGTCACAATTGAGCATCATTATTTTGGATGACATTGAGAGGTTGATGGAGTTTATTCCTATTGGTCCAAGATTTTCAACCATGATATATCAAACACTATGGGGCCTTCTTAAGCGCTTTCCTCCAAAGGGGAACAAGCTTCTGGTGATTGGAACAACTAGTGAATTTGAATTCTTGGACTCAATTCGCTTTTGCAAGACATTCTCTCACACTTACAATGTTCCTACATTAACTAGTGAGGATGCAAAGAAGGTGCTAGAACAGTTGAAAGTTTTTGCTGATGAGGATATAAATGCAGCTGCTGATGCCCTGAATGATATGCCTATCCAGAAGCTCTATACGTTAATTGAGACAGCAGGCCAATGTGCGAAGCCTGTTTACTCTGGAAAAAAGAAAATAAATATCAGTGACTTCTGTAATTGCCTTCGAGATATTGGGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

725

Amino Acids

80.75

Weight (kDa)

6.35

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 242 - 383 1.4e-34 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 410 - 446 2.2e-08 AAA+ lid domain
AAA PF00004 524 - 639 1.4e-10 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1122, 1332
AatII GACGTC 1 cut(s) 1126
Acc36I ACCTGC 1 cut(s) 1449
AccBSI CCGCTC 1 cut(s) 168
AccII CGCG 1 cut(s) 1401
AciI CCGC 7 cut(s) 24, 36, 103, 168, 251, 740, 1272
AclWI GGATC 1 cut(s) 794
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 5 cut(s) 783, 1137, 1185, 1898, 1904
AcuI CTGAAG 1 cut(s) 843
AcyI GRCGYC 1 cut(s) 1123
AfaI GTAC 3 cut(s) 374, 675, 748
AfeI AGCGCT 1 cut(s) 1850
AfiI CCNNNNNNNGG 6 cut(s) 154, 155, 749, 1427, 1576, 1862
AflII CTTAAG 2 cut(s) 1346, 1844
AhlI ACTAGT 2 cut(s) 1892, 1967
AjnI CCWGG 2 cut(s) 375, 1117
Alw21I GWGCWC 1 cut(s) 1214
Alw26I GTCTC 3 cut(s) 524, 1108, 2078
AlwI GGATC 1 cut(s) 794
AlwNI CAGNNNCTG 1 cut(s) 2036
Ama87I CYCGRG 1 cut(s) 254
Aor51HI AGCGCT 1 cut(s) 1850
AoxI GGCC 6 cut(s) 169, 817, 1147, 1579, 1837, 2092
ApaI GGGCCC 1 cut(s) 1583
ApeKI GCWGC 5 cut(s) 452, 727, 1610, 2030, 2033
ApoI RAATTY 5 cut(s) 783, 1137, 1185, 1898, 1904
AspLEI GCGC 2 cut(s) 1403, 1851
AspS9I GGNCC 6 cut(s) 737, 818, 1579, 1580, 1799, 1837
AsuC2I CCSGG 1 cut(s) 744
AsuHPI GGTGA 9 cut(s) 34, 54, 229, 233, 703, 857, 1496, 1697, 1891
AsuII TTCGAA 1 cut(s) 652
AvaI CYCGRG 1 cut(s) 254
AvaII GGWCC 2 cut(s) 737, 1799
BaeGI GKGCMC 1 cut(s) 1583
BanII GRGCYC 2 cut(s) 1214, 1583
BarI GAAGNNNNNNTAC 2 cut(s) 2096, 2128
BbsI GAAGAC 2 cut(s) 1670, 1711
Bbv12I GWGCWC 1 cut(s) 1214
BbvI GCAGC 5 cut(s) 439, 714, 1622, 2020, 2042
BccI CCATC 6 cut(s) 379, 410, 453, 1027, 1031, 1774
BceAI ACGGC 2 cut(s) 71, 966
BciT130I CCWGG 2 cut(s) 377, 1119
BcnI CCSGG 1 cut(s) 744
BcoDI GTCTC 3 cut(s) 524, 1108, 2078
BcuI ACTAGT 2 cut(s) 1892, 1967
BfaI CTAG 5 cut(s) 1574, 1584, 1893, 1968, 1991
BfmI CTRYAG 3 cut(s) 453, 1172, 1267
BfoI RGCGCY 1 cut(s) 1852
BfrI CTTAAG 2 cut(s) 1346, 1844
BfuAI ACCTGC 1 cut(s) 1449
BglI GCCNNNNNGGC 1 cut(s) 1587
BisI GCNGC 6 cut(s) 169, 453, 728, 1611, 2031, 2034
BlsI GCNGC 6 cut(s) 170, 454, 729, 1612, 2032, 2035
Bme1390I CCNGG 3 cut(s) 377, 744, 1119
Bme18I GGWCC 2 cut(s) 737, 1799
BmeT110I CYCGRG 1 cut(s) 254
BmgT120I GGNCC 6 cut(s) 737, 818, 1579, 1580, 1799, 1837
BmiI GGNNCC 5 cut(s) 976, 1047, 1432, 1581, 1838
BmrFI CCNGG 3 cut(s) 377, 744, 1119
BmsI GCATC 9 cut(s) 322, 469, 871, 940, 1445, 1632, 1758, 1966, 2029
BpiI GAAGAC 2 cut(s) 1670, 1711
Bpu10I CCTNAGC 1 cut(s) 1606
Bpu14I TTCGAA 1 cut(s) 652
BpuEI CTTGAG 1 cut(s) 266
BpuMI CCSGG 1 cut(s) 744
BsaBI GATNNNNATC 1 cut(s) 483
BsaHI GRCGYC 1 cut(s) 1123
BsaI GGTCTC 1 cut(s) 1108
BsaJI CCNNGG 2 cut(s) 255, 1434
Bsc4I CCNNNNNNNGG 6 cut(s) 154, 155, 749, 1427, 1576, 1862
Bse118I RCCGGY 1 cut(s) 26
Bse1I ACTGG 3 cut(s) 754, 1355, 1597
Bse3DI GCAATG 1 cut(s) 174
Bse8I GATNNNNATC 1 cut(s) 483
BseBI CCWGG 2 cut(s) 377, 1119
BseDI CCNNGG 2 cut(s) 255, 1434
BseGI GGATG 8 cut(s) 504, 916, 1042, 1105, 1393, 1623, 1768, 1981
BseJI GATNNNNATC 1 cut(s) 483
BseLI CCNNNNNNNGG 6 cut(s) 154, 155, 749, 1427, 1576, 1862
BseMI GCAATG 1 cut(s) 174
BseMII CTCAG 2 cut(s) 1322, 1653
BseNI ACTGG 3 cut(s) 754, 1355, 1597
BseRI GAGGAG 3 cut(s) 272, 1379, 1457
BseSI GKGCMC 1 cut(s) 1583
BseXI GCAGC 5 cut(s) 439, 714, 1622, 2020, 2042
BseYI CCCAGC 1 cut(s) 301
BsgI GTGCAG 1 cut(s) 1296
Bsh1236I CGCG 1 cut(s) 1401
Bsh1285I CGRYCG 1 cut(s) 103
BshFI GGCC 6 cut(s) 171, 819, 1149, 1581, 1839, 2094
BsiEI CGRYCG 1 cut(s) 103
BsiHKAI GWGCWC 1 cut(s) 1214
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 2 cut(s) 27, 744
BslFI GGGAC 2 cut(s) 74, 526
BslI CCNNNNNNNGG 6 cut(s) 154, 155, 749, 1427, 1576, 1862
BsmAI GTCTC 3 cut(s) 524, 1108, 2078
BsmFI GGGAC 2 cut(s) 74, 526
BsmI GAATGC 2 cut(s) 190, 996
BsnI GGCC 6 cut(s) 171, 819, 1149, 1581, 1839, 2094
Bso31I GGTCTC 1 cut(s) 1108
BsoBI CYCGRG 1 cut(s) 254
Bsp119I TTCGAA 1 cut(s) 652
Bsp120I GGGCCC 1 cut(s) 1579
Bsp1286I GDGCHC 2 cut(s) 1214, 1583
Bsp143I GATC 9 cut(s) 9, 70, 484, 799, 807, 892, 968, 1342, 1486
BspACI CCGC 7 cut(s) 24, 36, 103, 168, 251, 740, 1272
BspANI GGCC 6 cut(s) 171, 819, 1149, 1581, 1839, 2094
BspCNI CTCAG 2 cut(s) 1323, 1654
BspFNI CGCG 1 cut(s) 1401
BspHI TCATGA 2 cut(s) 1000, 1681
BspLI GGNNCC 5 cut(s) 976, 1047, 1432, 1581, 1838
BspMAI CTGCAG 1 cut(s) 457
BspMI ACCTGC 1 cut(s) 1449
BspPI GGATC 1 cut(s) 794
BspQI GCTCTTC 1 cut(s) 649
BspT104I TTCGAA 1 cut(s) 652
BspTI CTTAAG 2 cut(s) 1346, 1844
BspTNI GGTCTC 1 cut(s) 1108
BsrBI CCGCTC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 174
BsrFI RCCGGY 1 cut(s) 26
BsrI ACTGG 3 cut(s) 754, 1355, 1597
BssAI RCCGGY 1 cut(s) 26
BssECI CCNNGG 2 cut(s) 255, 1434
BssMI GATC 9 cut(s) 9, 70, 484, 799, 807, 892, 968, 1342, 1486
BssNI GRCGYC 1 cut(s) 1123
BssT1I CCWWGG 1 cut(s) 1434
Bst2UI CCWGG 2 cut(s) 377, 1119
Bst4CI ACNGT 5 cut(s) 66, 118, 442, 836, 1998
Bst6I CTCTTC 1 cut(s) 649
BstACI GRCGYC 1 cut(s) 1123
BstAFI CTTAAG 2 cut(s) 1346, 1844
BstBI TTCGAA 1 cut(s) 652
BstC8I GCNNGC 6 cut(s) 28, 204, 725, 1248, 1500, 2092
BstDEI CTNAG 4 cut(s) 356, 1331, 1606, 1662
BstEII GGTNACC 1 cut(s) 221
BstENI CCTNNNNNAGG 1 cut(s) 1574
BstF5I GGATG 8 cut(s) 504, 916, 1042, 1105, 1393, 1623, 1768, 1981
BstFNI CGCG 1 cut(s) 1401
BstH2I RGCGCY 1 cut(s) 1852
BstHHI GCGC 2 cut(s) 1403, 1851
BstKTI GATC 9 cut(s) 12, 73, 487, 802, 810, 895, 971, 1345, 1489
BstMAI GTCTC 3 cut(s) 524, 1108, 2078
BstMBI GATC 9 cut(s) 9, 70, 484, 799, 807, 892, 968, 1342, 1486
BstMCI CGRYCG 1 cut(s) 103
BstMWI GCNNNNNNNGC 6 cut(s) 185, 1256, 1587, 1610, 1629, 2039
BstNI CCWGG 2 cut(s) 377, 1119
BstNSI RCATGY 3 cut(s) 716, 727, 929
BstPI GGTNACC 1 cut(s) 221
BstSCI CCNGG 3 cut(s) 375, 742, 1117
BstSFI CTRYAG 3 cut(s) 453, 1172, 1267
BstSLI GKGCMC 1 cut(s) 1583
BstUI CGCG 1 cut(s) 1401
BstV1I GCAGC 5 cut(s) 439, 714, 1622, 2020, 2042
BstV2I GAAGAC 2 cut(s) 1670, 1711
BstX2I RGATCY 1 cut(s) 799
BstYI RGATCY 1 cut(s) 799
BsuRI GGCC 6 cut(s) 171, 819, 1149, 1581, 1839, 2094
BtsCI GGATG 8 cut(s) 504, 916, 1042, 1105, 1393, 1623, 1768, 1981
BtsI GCAGTG 1 cut(s) 275
BtsIMutI CAGTG 4 cut(s) 275, 1362, 1590, 2146
BveI ACCTGC 1 cut(s) 1449
Cac8I GCNNGC 6 cut(s) 28, 204, 725, 1248, 1500, 2092
CaiI CAGNNNCTG 1 cut(s) 2036
CciI TCATGA 2 cut(s) 1000, 1681
CfoI GCGC 2 cut(s) 1403, 1851
Cfr10I RCCGGY 1 cut(s) 26
Cfr13I GGNCC 6 cut(s) 737, 818, 1579, 1580, 1799, 1837
CsiI ACCWGGT 1 cut(s) 375
Csp6I GTAC 3 cut(s) 373, 674, 747
CviQI GTAC 3 cut(s) 373, 674, 747
DdeI CTNAG 4 cut(s) 356, 1331, 1606, 1662
DpnI GATC 9 cut(s) 11, 72, 486, 801, 809, 894, 970, 1344, 1488
DpnII GATC 9 cut(s) 9, 70, 484, 799, 807, 892, 968, 1342, 1486
DrdI GACNNNNNNGTC 2 cut(s) 1122, 1332
DseDI GACNNNNNNGTC 2 cut(s) 1122, 1332
EaeI YGGCCR 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 649
EarI CTCTTC 1 cut(s) 649
EciI GGCGGA 2 cut(s) 25, 240
Ecl136II GAGCTC 1 cut(s) 1212
Eco130I CCWWGG 1 cut(s) 1434
Eco147I AGGCCT 1 cut(s) 1149
Eco24I GRGCYC 2 cut(s) 1214, 1583
Eco31I GGTCTC 1 cut(s) 1108
Eco47I GGWCC 2 cut(s) 737, 1799
Eco47III AGCGCT 1 cut(s) 1850
Eco53kI GAGCTC 1 cut(s) 1212
Eco57I CTGAAG 1 cut(s) 843
Eco88I CYCGRG 1 cut(s) 254
Eco91I GGTNACC 1 cut(s) 221
EcoICRI GAGCTC 1 cut(s) 1212
EcoNI CCTNNNNNAGG 1 cut(s) 1574
EcoO109I RGGNCCY 3 cut(s) 1579, 1580, 1837
EcoO65I GGTNACC 1 cut(s) 221
EcoRI GAATTC 1 cut(s) 1904
EcoRII CCWGG 2 cut(s) 375, 1117
EcoT14I CCWWGG 1 cut(s) 1434
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 1214, 1583
ErhI CCWWGG 1 cut(s) 1434
FaqI GGGAC 2 cut(s) 74, 526
FauI CCCGC 1 cut(s) 31
FauNDI CATATG 1 cut(s) 6
Fnu4HI GCNGC 6 cut(s) 169, 453, 728, 1611, 2031, 2034
FokI GGATG 8 cut(s) 511, 923, 1049, 1112, 1400, 1610, 1775, 1988
FriOI GRGCYC 2 cut(s) 1214, 1583
Fsp4HI GCNGC 6 cut(s) 169, 453, 728, 1611, 2031, 2034
FspBI CTAG 5 cut(s) 1574, 1584, 1893, 1968, 1991
GlaI GCGC 2 cut(s) 1402, 1850
GluI GCNGC 6 cut(s) 169, 453, 728, 1611, 2031, 2034
GsaI CCCAGC 1 cut(s) 305
HaeII RGCGCY 1 cut(s) 1852
HaeIII GGCC 6 cut(s) 171, 819, 1149, 1581, 1839, 2094
HapII CCGG 2 cut(s) 27, 744
HhaI GCGC 2 cut(s) 1403, 1851
Hin1I GRCGYC 1 cut(s) 1123
Hin6I GCGC 2 cut(s) 1401, 1849
HinP1I GCGC 2 cut(s) 1401, 1849
HindIII AAGCTT 2 cut(s) 1728, 1871
HinfI GANTC 5 cut(s) 32, 1177, 1333, 1664, 1913
HpaII CCGG 2 cut(s) 27, 744
HphI GGTGA 9 cut(s) 34, 54, 229, 233, 703, 857, 1496, 1697, 1891
Hpy166II GTNNAC 7 cut(s) 674, 992, 1015, 1234, 1360, 1705, 2113
Hpy188I TCNGA 6 cut(s) 70, 100, 183, 415, 1065, 1143
Hpy8I GTNNAC 7 cut(s) 674, 992, 1015, 1234, 1360, 1705, 2113
Hpy99I CGWCG 3 cut(s) 122, 350, 1401
HpyCH4III ACNGT 5 cut(s) 66, 118, 442, 836, 1998
HpyCH4IV ACGT 7 cut(s) 132, 432, 571, 840, 1123, 1716, 2075
HpyF10VI GCNNNNNNNGC 6 cut(s) 185, 1256, 1587, 1610, 1629, 2039
HpyF3I CTNAG 4 cut(s) 356, 1331, 1606, 1662
HpySE526I ACGT 7 cut(s) 132, 432, 571, 840, 1123, 1716, 2075
Hsp92I GRCGYC 1 cut(s) 1123
HspAI GCGC 2 cut(s) 1401, 1849
KroI GCCGGC 1 cut(s) 26
KroNI GCCGGC 1 cut(s) 28
Kzo9I GATC 9 cut(s) 9, 70, 484, 799, 807, 892, 968, 1342, 1486
LguI GCTCTTC 1 cut(s) 649
LmnI GCTCC 6 cut(s) 868, 1045, 1228, 1430, 1444, 1528
Lsp1109I GCAGC 5 cut(s) 439, 714, 1622, 2020, 2042
LweI GCATC 9 cut(s) 322, 469, 871, 940, 1445, 1632, 1758, 1966, 2029
MabI ACCWGGT 1 cut(s) 375
MaeI CTAG 5 cut(s) 1574, 1584, 1893, 1968, 1991
MaeII ACGT 7 cut(s) 132, 432, 571, 840, 1123, 1716, 2075
MaeIII GTNAC 8 cut(s) 13, 221, 235, 351, 836, 1378, 1737, 2141
MalI GATC 9 cut(s) 11, 72, 486, 801, 809, 894, 970, 1344, 1488
MbiI CCGCTC 1 cut(s) 168
MboI GATC 9 cut(s) 9, 70, 484, 799, 807, 892, 968, 1342, 1486
MboII GAAGA 9 cut(s) 65, 517, 571, 593, 666, 868, 1172, 1670, 1711
MfeI CAATTG 1 cut(s) 1742
MflI RGATCY 1 cut(s) 799
MhlI GDGCHC 2 cut(s) 1214, 1583
MlyI GAGTC 4 cut(s) 26, 1342, 1673, 1907
MmeI TCCRAC 2 cut(s) 123, 810
Mph1103I ATGCAT 1 cut(s) 6
MreI CGCCGGCG 1 cut(s) 26
MroNI GCCGGC 1 cut(s) 26
MslI CAYNNNNRTG 2 cut(s) 909, 1503
MspA1I CMGCKG 2 cut(s) 105, 2033
MspCI CTTAAG 2 cut(s) 1346, 1844
MspI CCGG 2 cut(s) 27, 744
MspR9I CCNGG 3 cut(s) 377, 744, 1119
MunI CAATTG 1 cut(s) 1742
Mva1269I GAATGC 2 cut(s) 190, 996
MvaI CCWGG 2 cut(s) 377, 1119
MvnI CGCG 1 cut(s) 1401
MwoI GCNNNNNNNGC 6 cut(s) 185, 1256, 1587, 1610, 1629, 2039
NaeI GCCGGC 1 cut(s) 28
NciI CCSGG 1 cut(s) 744
NdeI CATATG 1 cut(s) 6
NdeII GATC 9 cut(s) 9, 70, 484, 799, 807, 892, 968, 1342, 1486
NgoMIV GCCGGC 1 cut(s) 26
NlaIV GGNNCC 5 cut(s) 976, 1047, 1432, 1581, 1838
NmeAIII GCCGAG 1 cut(s) 748
NmuCI GTSAC 4 cut(s) 221, 235, 1737, 2141
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 716, 727, 929
NspV TTCGAA 1 cut(s) 652
PaeI GCATGC 1 cut(s) 727
PagI TCATGA 2 cut(s) 1000, 1681
PceI AGGCCT 1 cut(s) 1149
PciSI GCTCTTC 1 cut(s) 649
PctI GAATGC 2 cut(s) 190, 996
PdiI GCCGGC 1 cut(s) 28
PfeI GAWTC 1 cut(s) 1177
PkrI GCNGC 6 cut(s) 170, 454, 729, 1612, 2032, 2035
PleI GAGTC 4 cut(s) 26, 1341, 1672, 1907
PpsI GAGTC 4 cut(s) 26, 1341, 1672, 1907
Psp124BI GAGCTC 1 cut(s) 1214
Psp6I CCWGG 2 cut(s) 375, 1117
PspEI GGTNACC 1 cut(s) 221
PspFI CCCAGC 1 cut(s) 301
PspGI CCWGG 2 cut(s) 375, 1117
PspN4I GGNNCC 5 cut(s) 976, 1047, 1432, 1581, 1838
PspOMI GGGCCC 1 cut(s) 1579
PspPI GGNCC 6 cut(s) 737, 818, 1579, 1580, 1799, 1837
PstI CTGCAG 1 cut(s) 457
PstNI CAGNNNCTG 1 cut(s) 2036
PsuI RGATCY 1 cut(s) 799
PvuII CAGCTG 1 cut(s) 2033
RsaI GTAC 3 cut(s) 374, 675, 748
RsaNI GTAC 3 cut(s) 373, 674, 747
RseI CAYNNNNRTG 2 cut(s) 909, 1503
SacI GAGCTC 1 cut(s) 1214
SapI GCTCTTC 1 cut(s) 649
SatI GCNGC 6 cut(s) 169, 453, 728, 1611, 2031, 2034
Sau3AI GATC 9 cut(s) 9, 70, 484, 799, 807, 892, 968, 1342, 1486
Sau96I GGNCC 6 cut(s) 737, 818, 1579, 1580, 1799, 1837
SchI GAGTC 4 cut(s) 26, 1342, 1673, 1907
ScrFI CCNGG 3 cut(s) 377, 744, 1119
SduI GDGCHC 2 cut(s) 1214, 1583
SexAI ACCWGGT 1 cut(s) 375
SfaNI GCATC 9 cut(s) 322, 469, 871, 940, 1445, 1632, 1758, 1966, 2029
SfcI CTRYAG 3 cut(s) 453, 1172, 1267
SfuI TTCGAA 1 cut(s) 652
SgrAI CRCCGGYG 1 cut(s) 26
SinI GGWCC 2 cut(s) 737, 1799
SmiMI CAYNNNNRTG 2 cut(s) 909, 1503
SmlI CTYRAG 3 cut(s) 281, 1346, 1844
SmoI CTYRAG 3 cut(s) 281, 1346, 1844
SpeI ACTAGT 2 cut(s) 1892, 1967
SphI GCATGC 1 cut(s) 727
SseBI AGGCCT 1 cut(s) 1149
SsiI CCGC 7 cut(s) 24, 36, 103, 168, 251, 740, 1272
SspI AATATT 4 cut(s) 936, 1009, 1060, 1372
SspMI CTAG 5 cut(s) 1574, 1584, 1893, 1968, 1991
SstI GAGCTC 1 cut(s) 1214
StuI AGGCCT 1 cut(s) 1149
StyD4I CCNGG 3 cut(s) 375, 742, 1117
StyI CCWWGG 1 cut(s) 1434
TaaI ACNGT 5 cut(s) 66, 118, 442, 836, 1998
TaiI ACGT 7 cut(s) 135, 435, 574, 843, 1126, 1719, 2078
TaqI TCGA 9 cut(s) 322, 330, 345, 528, 652, 1240, 1489, 1722, 2161
TatI WGTACW 1 cut(s) 673
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 1 cut(s) 1177
TscAI CASTG 4 cut(s) 282, 1362, 1597, 2146
TseFI GTSAC 4 cut(s) 221, 235, 1737, 2141
TseI GCWGC 5 cut(s) 452, 727, 1610, 2030, 2033
Tsp45I GTSAC 4 cut(s) 221, 235, 1737, 2141
TspRI CASTG 4 cut(s) 282, 1362, 1597, 2146
Vha464I CTTAAG 2 cut(s) 1346, 1844
VpaK11BI GGWCC 2 cut(s) 737, 1799
XagI CCTNNNNNAGG 1 cut(s) 1574
XapI RAATTY 5 cut(s) 783, 1137, 1185, 1898, 1904
XbaI TCTAGA 1 cut(s) 1573
XceI RCATGY 3 cut(s) 716, 727, 929
XspI CTAG 5 cut(s) 1574, 1584, 1893, 1968, 1991
ZraI GACGTC 1 cut(s) 1124
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.