Rw5G032430

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001309
Physical Location & Seq
Reverse (-)
54884532 .. 54894041
9510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G032430.1

Sequence Viewer

Length: 1113 bp
ATGAAAATTGCTTCATACAACATGTTTAAGGAGCTTCCGTTGTGCGAAGATGTTACAATTCAGAATTTTTTAGATAGAAAAACATTGTACAACACTTATAAGGATGATTCATTCGGCAATGTTCCTCCTTACTGCCTCAGCTCAAATTTAATGTCTTCAGAATCAGACAACATAACTTCAATTTATGTAAATTTCATTCCCTCTCGATCGAGTCCTGAGGATGCTCAGCTTGCTACTTTTTTCACTTGCCTGCATCATAAGTTCAATACGACCTTGAAGTCATTGGAATCGTTTCAAGCTAAGAACTCTGAGCGTGTGTTCAATACAGTTATGACTTACATTCCTCAAGATTTTCGATGGAGGCTTATTAGACTGCAGAGGGAGCGATCCGAGAGGAATGGGATTGTTCATGGCCCTTCCAGGAGGAGACTATTGGCACAGCTTGGTTCTGCTACTTGTGTCTACAAAACCCTTGTCAAGTACTTGGTTGGGGTTCCACAGGTTTTACTAGATTTTGTTCGCCAAATAAATGATGATGATGGGCCCATGGAAGAGCAACGACAGCATTATGGACCACCTTTGTACCGCCTTACAACTTTGGTGCTTCTTATACGTCTGGCTATTTTGCTGTCTCGGGGGCGGTTTGAAACTAGAAAATTAAACAAGCAAGAGTTAGCTGTCTTGGAACAATCTGTTGATGTCTACACCACTGAGTTTGAAAGGTTTATTAGGTTTATCAAGAATAATGATGATTACAAAGAGACAACTGTTCCTGACGGTAAAACACATGAGGTTTCTCTGTCTGTGGACTTGATAAATTCGTATATTGCTTGGGATTTTTCGCTTGTGCAAGGGAAGATAAACTTGGTATCAATGGTTAACTCTATATACTTTTTTGTTTCATACATAAATGATCACTATATCAATTCGAAATTATCATACAAAAGATTTCTATCATGTTTGATTACTCATTTGAGCATTTGCAATGTGTTTTCTTTCCAATTGATCAACGATCTAGACCTATCACACAACAATGATAACACATACCTGTTGTTTGTGTGTTTCTCACACAACTGTGTTCCACCGGCTCACTCTACAATGGTGGGTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

370

Amino Acids

42.94

Weight (kDa)

7.6

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 99
AasI GACNNNNNNGTC 1 cut(s) 277
AccI GTMKAC 2 cut(s) 462, 702
AciI CCGC 2 cut(s) 586, 640
AclWI GGATC 1 cut(s) 381
AcsI RAATTY 4 cut(s) 64, 145, 190, 817
AcuI CTGAAG 1 cut(s) 141
AfaI GTAC 3 cut(s) 89, 482, 584
AflIII ACRYGT 1 cut(s) 21
AgsI TTSAA 7 cut(s) 180, 265, 277, 296, 322, 647, 719
AjnI CCWGG 1 cut(s) 419
AjuI GAANNNNNNNTTGG 2 cut(s) 848, 880
AleI CACNNNNGTG 1 cut(s) 1074
AluBI AGCT 6 cut(s) 34, 141, 229, 299, 442, 677
AluI AGCT 6 cut(s) 34, 141, 229, 299, 442, 677
Alw26I GTCTC 3 cut(s) 421, 636, 755
AlwI GGATC 1 cut(s) 381
Ama87I CYCGRG 1 cut(s) 633
AoxI GGCC 2 cut(s) 412, 542
ApaI GGGCCC 1 cut(s) 546
ApoI RAATTY 4 cut(s) 64, 145, 190, 817
ArsI GACNNNNNNTTYG 4 cut(s) 137, 169, 459, 491
Asp700I GAANNNNTTC 1 cut(s) 291
AspS9I GGNCC 4 cut(s) 413, 542, 543, 572
AsuII TTCGAA 1 cut(s) 929
AvaI CYCGRG 1 cut(s) 633
AvaII GGWCC 1 cut(s) 572
AxyI CCTNAGG 1 cut(s) 216
BaeGI GKGCMC 1 cut(s) 546
BaeI ACNNNNGTAYC 2 cut(s) 566, 599
BanII GRGCYC 1 cut(s) 546
BbsI GAAGAC 1 cut(s) 147
BbvCI CCTCAGC 1 cut(s) 137
BccI CCATC 2 cut(s) 351, 533
BciT130I CCWGG 1 cut(s) 421
BclI TGATCA 2 cut(s) 913, 1005
BcoDI GTCTC 3 cut(s) 421, 636, 755
BfaI CTAG 3 cut(s) 509, 651, 1016
BfmI CTRYAG 1 cut(s) 374
BlpI GCTNAGC 1 cut(s) 225
BmcAI AGTACT 1 cut(s) 482
Bme1390I CCNGG 1 cut(s) 421
Bme18I GGWCC 1 cut(s) 572
BmeT110I CYCGRG 1 cut(s) 633
BmgT120I GGNCC 4 cut(s) 413, 542, 543, 572
BmiI GGNNCC 2 cut(s) 495, 544
BmrFI CCNGG 1 cut(s) 421
BmsI GCATC 2 cut(s) 211, 262
BpiI GAAGAC 1 cut(s) 147
Bpu10I CCTNAGC 1 cut(s) 137
Bpu1102I GCTNAGC 1 cut(s) 225
Bpu14I TTCGAA 1 cut(s) 929
BpuEI CTTGAG 1 cut(s) 330
BsaBI GATNNNNATC 1 cut(s) 952
BsaJI CCNNGG 1 cut(s) 546
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bse118I RCCGGY 1 cut(s) 1084
Bse21I CCTNAGG 1 cut(s) 216
Bse3DI GCAATG 2 cut(s) 124, 991
Bse8I GATNNNNATC 1 cut(s) 952
BseBI CCWGG 1 cut(s) 421
BseDI CCNNGG 1 cut(s) 546
BseGI GGATG 2 cut(s) 109, 226
BseJI GATNNNNATC 1 cut(s) 952
BseMI GCAATG 2 cut(s) 124, 991
BseMII CTCAG 5 cut(s) 151, 207, 239, 300, 702
BseRI GAGGAG 1 cut(s) 439
BseSI GKGCMC 1 cut(s) 546
Bsh1285I CGRYCG 1 cut(s) 209
BshFI GGCC 2 cut(s) 414, 544
BsiEI CGRYCG 1 cut(s) 209
BsiHKCI CYCGRG 1 cut(s) 633
BsiSI CCGG 1 cut(s) 1085
BsmAI GTCTC 3 cut(s) 421, 636, 755
BsnI GGCC 2 cut(s) 414, 544
BsoBI CYCGRG 1 cut(s) 633
Bsp119I TTCGAA 1 cut(s) 929
Bsp120I GGGCCC 1 cut(s) 542
Bsp1286I GDGCHC 1 cut(s) 546
Bsp1407I TGTACA 1 cut(s) 87
Bsp143I GATC 5 cut(s) 206, 386, 913, 1005, 1012
Bsp1720I GCTNAGC 1 cut(s) 225
Bsp19I CCATGG 1 cut(s) 546
BspACI CCGC 2 cut(s) 586, 640
BspANI GGCC 2 cut(s) 414, 544
BspCNI CTCAG 5 cut(s) 150, 208, 238, 301, 703
BspLI GGNNCC 2 cut(s) 495, 544
BspMAI CTGCAG 1 cut(s) 378
BspPI GGATC 1 cut(s) 381
BspQI GCTCTTC 1 cut(s) 546
BspT104I TTCGAA 1 cut(s) 929
BsrDI GCAATG 2 cut(s) 124, 991
BsrFI RCCGGY 1 cut(s) 1084
BsrGI TGTACA 1 cut(s) 87
BssAI RCCGGY 1 cut(s) 1084
BssECI CCNNGG 1 cut(s) 546
BssMI GATC 5 cut(s) 206, 386, 913, 1005, 1012
BssT1I CCWWGG 1 cut(s) 546
Bst2UI CCWGG 1 cut(s) 421
Bst4CI ACNGT 4 cut(s) 328, 769, 779, 1076
Bst6I CTCTTC 1 cut(s) 546
BstAUI TGTACA 1 cut(s) 87
BstBI TTCGAA 1 cut(s) 929
BstC8I GCNNGC 2 cut(s) 231, 251
BstDEI CTNAG 6 cut(s) 137, 216, 225, 300, 309, 711
BstDSI CCRYGG 1 cut(s) 546
BstF5I GGATG 2 cut(s) 109, 226
BstKTI GATC 5 cut(s) 209, 389, 916, 1008, 1015
BstMAI GTCTC 3 cut(s) 421, 636, 755
BstMBI GATC 5 cut(s) 206, 386, 913, 1005, 1012
BstMCI CGRYCG 1 cut(s) 209
BstMWI GCNNNNNNNGC 3 cut(s) 230, 382, 562
BstNI CCWGG 1 cut(s) 421
BstNSI RCATGY 1 cut(s) 25
BstSCI CCNGG 1 cut(s) 419
BstSFI CTRYAG 1 cut(s) 374
BstSLI GKGCMC 1 cut(s) 546
BstV2I GAAGAC 1 cut(s) 147
Bsu36I CCTNAGG 1 cut(s) 216
BsuRI GGCC 2 cut(s) 414, 544
BtgI CCRYGG 1 cut(s) 546
BtsCI GGATG 2 cut(s) 109, 226
BtsIMutI CAGTG 1 cut(s) 708
Cac8I GCNNGC 2 cut(s) 231, 251
Cfr10I RCCGGY 1 cut(s) 1084
Cfr13I GGNCC 4 cut(s) 413, 542, 543, 572
Csp6I GTAC 3 cut(s) 88, 481, 583
CspCI CAANNNNNGTGG 2 cut(s) 697, 732
CviAII CATG 5 cut(s) 22, 410, 547, 788, 957
CviQI GTAC 3 cut(s) 88, 481, 583
DdeI CTNAG 6 cut(s) 137, 216, 225, 300, 309, 711
DpnI GATC 5 cut(s) 208, 388, 915, 1007, 1014
DpnII GATC 5 cut(s) 206, 386, 913, 1005, 1012
DrdI GACNNNNNNGTC 1 cut(s) 277
DseDI GACNNNNNNGTC 1 cut(s) 277
Eam1104I CTCTTC 1 cut(s) 546
EarI CTCTTC 1 cut(s) 546
Eco130I CCWWGG 1 cut(s) 546
Eco24I GRGCYC 1 cut(s) 546
Eco47I GGWCC 1 cut(s) 572
Eco57I CTGAAG 1 cut(s) 141
Eco81I CCTNAGG 1 cut(s) 216
Eco88I CYCGRG 1 cut(s) 633
EcoRII CCWGG 1 cut(s) 419
EcoT14I CCWWGG 1 cut(s) 546
EcoT38I GRGCYC 1 cut(s) 546
ErhI CCWWGG 1 cut(s) 546
FaeI CATG 5 cut(s) 25, 413, 550, 791, 960
FalI AAGNNNNNCTT 2 cut(s) 848, 880
FatI CATG 5 cut(s) 21, 409, 546, 787, 956
FbaI TGATCA 2 cut(s) 913, 1005
FblI GTMKAC 2 cut(s) 462, 702
FokI GGATG 2 cut(s) 116, 233
FriOI GRGCYC 1 cut(s) 546
FspBI CTAG 3 cut(s) 509, 651, 1016
HaeIII GGCC 2 cut(s) 414, 544
HapII CCGG 1 cut(s) 1085
Hin1II CATG 5 cut(s) 25, 413, 550, 791, 960
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HinfI GANTC 4 cut(s) 107, 161, 211, 287
HpaI GTTAAC 1 cut(s) 880
HpaII CCGG 1 cut(s) 1085
Hpy166II GTNNAC 4 cut(s) 463, 703, 808, 880
Hpy188I TCNGA 5 cut(s) 63, 160, 166, 310, 391
Hpy188III TCNNGA 6 cut(s) 204, 215, 347, 739, 773, 1016
Hpy8I GTNNAC 4 cut(s) 463, 703, 808, 880
HpyAV CCTTC 1 cut(s) 426
HpyCH4III ACNGT 4 cut(s) 328, 769, 779, 1076
HpyCH4IV ACGT 1 cut(s) 613
HpyCH4V TGCA 4 cut(s) 253, 376, 850, 984
HpyF10VI GCNNNNNNNGC 3 cut(s) 230, 382, 562
HpyF3I CTNAG 6 cut(s) 137, 216, 225, 300, 309, 711
HpySE526I ACGT 1 cut(s) 613
Hsp92II CATG 5 cut(s) 25, 413, 550, 791, 960
Ksp22I TGATCA 2 cut(s) 913, 1005
KspAI GTTAAC 1 cut(s) 880
Kzo9I GATC 5 cut(s) 206, 386, 913, 1005, 1012
LguI GCTCTTC 1 cut(s) 546
LmnI GCTCC 2 cut(s) 31, 382
LpnPI CCDG 9 cut(s) 228, 263, 406, 433, 485, 602, 786, 1061, 1098
LweI GCATC 2 cut(s) 211, 262
MaeI CTAG 3 cut(s) 509, 651, 1016
MaeII ACGT 1 cut(s) 613
MaeIII GTNAC 1 cut(s) 52
MalI GATC 5 cut(s) 208, 388, 915, 1007, 1014
MboI GATC 5 cut(s) 206, 386, 913, 1005, 1012
MboII GAAGA 4 cut(s) 59, 147, 563, 868
MfeI CAATTG 1 cut(s) 1001
MhlI GDGCHC 1 cut(s) 546
MlyI GAGTC 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 291
MseI TTAA 4 cut(s) 27, 149, 659, 879
MslI CAYNNNNRTG 2 cut(s) 1032, 1074
MspI CCGG 1 cut(s) 1085
MspR9I CCNGG 1 cut(s) 421
MunI CAATTG 1 cut(s) 1001
MvaI CCWGG 1 cut(s) 421
MwoI GCNNNNNNNGC 3 cut(s) 230, 382, 562
NcoI CCATGG 1 cut(s) 546
NdeII GATC 5 cut(s) 206, 386, 913, 1005, 1012
NlaIII CATG 5 cut(s) 25, 413, 550, 791, 960
NlaIV GGNNCC 2 cut(s) 495, 544
NspI RCATGY 1 cut(s) 25
NspV TTCGAA 1 cut(s) 929
OliI CACNNNNGTG 1 cut(s) 1074
PciI ACATGT 1 cut(s) 21
PciSI GCTCTTC 1 cut(s) 546
PdmI GAANNNNTTC 1 cut(s) 291
PfeI GAWTC 3 cut(s) 107, 161, 287
PfoI TCCNGGA 1 cut(s) 419
Ple19I CGATCG 1 cut(s) 209
PleI GAGTC 1 cut(s) 219
PpsI GAGTC 1 cut(s) 219
PscI ACATGT 1 cut(s) 21
PsiI TTATAA 1 cut(s) 99
Psp6I CCWGG 1 cut(s) 419
PspGI CCWGG 1 cut(s) 419
PspN4I GGNNCC 2 cut(s) 495, 544
PspOMI GGGCCC 1 cut(s) 542
PspPI GGNCC 4 cut(s) 413, 542, 543, 572
PstI CTGCAG 1 cut(s) 378
PvuI CGATCG 1 cut(s) 209
RsaI GTAC 3 cut(s) 89, 482, 584
RsaNI GTAC 3 cut(s) 88, 481, 583
RseI CAYNNNNRTG 2 cut(s) 1032, 1074
SapI GCTCTTC 1 cut(s) 546
SaqAI TTAA 4 cut(s) 27, 149, 659, 879
Sau3AI GATC 5 cut(s) 206, 386, 913, 1005, 1012
Sau96I GGNCC 4 cut(s) 413, 542, 543, 572
ScaI AGTACT 1 cut(s) 482
SchI GAGTC 1 cut(s) 220
ScrFI CCNGG 1 cut(s) 421
SduI GDGCHC 1 cut(s) 546
SfaNI GCATC 2 cut(s) 211, 262
SfcI CTRYAG 1 cut(s) 374
SfuI TTCGAA 1 cut(s) 929
SinI GGWCC 1 cut(s) 572
SmiMI CAYNNNNRTG 2 cut(s) 1032, 1074
SmlI CTYRAG 1 cut(s) 345
SmoI CTYRAG 1 cut(s) 345
SsiI CCGC 2 cut(s) 586, 640
SspMI CTAG 3 cut(s) 509, 651, 1016
StyD4I CCNGG 1 cut(s) 419
StyI CCWWGG 1 cut(s) 546
TaaI ACNGT 4 cut(s) 328, 769, 779, 1076
TaiI ACGT 1 cut(s) 616
TaqI TCGA 4 cut(s) 205, 209, 355, 929
TatI WGTACW 2 cut(s) 87, 480
TfiI GAWTC 3 cut(s) 107, 161, 287
Tru1I TTAA 4 cut(s) 27, 149, 659, 879
Tru9I TTAA 4 cut(s) 27, 149, 659, 879
TscAI CASTG 1 cut(s) 715
TspDTI ATGAA 5 cut(s) 17, 99, 184, 398, 891
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 1 cut(s) 715
VpaK11BI GGWCC 1 cut(s) 572
XapI RAATTY 4 cut(s) 64, 145, 190, 817
XbaI TCTAGA 1 cut(s) 1015
XceI RCATGY 1 cut(s) 25
XmiI GTMKAC 2 cut(s) 462, 702
XmnI GAANNNNTTC 1 cut(s) 291
XspI CTAG 3 cut(s) 509, 651, 1016
ZrmI AGTACT 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.