MD12G1196200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001309
Physical Location & Seq
Forward (+)
27715587 .. 27724029
8443 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1196200.v1.1.491

Sequence Viewer

Length: 1323 bp
ATGGCTTCTACGGAGGGTATAGTACCAATAACCAGGGCTTTTCTCGCGTCCTACTACGACACGTACCAATTCCCCGCTCTCTCAAACGACGTCGCTCGCCTCTCCTCCGAGATTCGCTCCTTCACCGCCGATTTGCTCCACCACTCTCCGGCGGCTCAAGGCGAGGAGAGATTGTTAGTGAGTGAATTGGAGAGTGAACCGCCGCACAAAGTCGACGAAAACATGTGGAAGAATCGAGAGCATATGGAGGAGATACTGTTTTTGCTTGAAAAACCCCATTGGCCTCAAACTCTTCGAGAGCAGTCGAGTCCTGATGATGCTGAACTTGCTACTGTGATTGATCGCCTCCGCCAGAAATTTCAAACCAGTTTGAAGACACTGGAGTCTTTCCAAGCGAAAAATTCCGAACGTGTGTTCAACACGGTTATGACTTACATTCCTCAGGATTTTCGGGGGAGGCTCATCAGACAACAGCGGGAGCGTTCAGAGAGGAATAAGCAAGCAGAGGTTGAAGCTTTGGTTAGTTCTGGGGCAACTATACGTGAACGTTATGCTCTCTTGTGGAATCAGCAAATGGAGAGGAGGAGACTGTTAGCGCAGCTTGGTTCTGCAACTGGTGTCTACAAGACGCTTGTCAAGTACTTGGTTGGGGTTCCACAGGTTCTACTTGATTTTATTTGCCAAATAAATGATGATGATGGGCCCATGGAAGAGCAACGGCAACGTTATGGACCCCCTTTATACAGCCTTACAACTATGGTGCTTCTTATACGACTCCTTATTTCGCTGTCTTGGGGGCGCTTTGAGAATAGAAAATTAAAGAAGCCAGAGTTAGCTGTCTTGGAAGAAGCTGTTGATGTCTACACCACTGAGTTTGAAAGGTTTATTACGTTCATCAGTGAGGTCTTTGCAAATTCCCCCTTTTTTATCCCAGCAGAGGTTGCGGGCGCATTAGAAGGAAGGAATAATGATGATTACCAAGAGACGAGTGTTCCAGCTGGGAAAACTCATGAGGTTTTATTGGCTGTGGATGCGATAAATTCATATATTGCTTGGGATTTCTCGCTTGTACAGGGGAAGATAAACTTGGATATTGGATTTAGTGTCGAGTACACAAATTCTTCTGGGGAAAAATCTCTCATTTTACCTTACCGACGCTACGATTCTGACCAAGGAAACTTCTGCACATGCGAGGCTGGAATCTACAAACTGATTTGGGACAATTCCTATTCAAATTTTTTTAAGAAGGTTCTGCGCTTCAAGGTAGATTGCATACCGCCAGTTGCAGAGTCGGTGCAAACTGCGAACGAGGTTGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

441

Amino Acids

50.53

Weight (kDa)

5.1

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 382
AatII GACGTC 1 cut(s) 93
AccBSI CCGCTC 1 cut(s) 77
AccI GTMKAC 3 cut(s) 213, 621, 861
AccII CGCG 1 cut(s) 47
AciI CCGC 9 cut(s) 75, 126, 152, 200, 203, 349, 475, 944, 1277
AclI AACGTT 2 cut(s) 547, 724
AcsI RAATTY 6 cut(s) 356, 400, 913, 1039, 1117, 1234
AcyI GRCGYC 1 cut(s) 90
AfaI GTAC 5 cut(s) 24, 65, 641, 1071, 1112
AfiI CCNNNNNNNGG 2 cut(s) 148, 937
AflIII ACRYGT 3 cut(s) 60, 222, 409
AgsI TTSAA 9 cut(s) 269, 362, 373, 418, 512, 878, 1233, 1261, 1316
AjnI CCWGG 1 cut(s) 32
AjuI GAANNNNNNNTTGG 2 cut(s) 1070, 1102
AluBI AGCT 5 cut(s) 515, 601, 836, 851, 998
AluI AGCT 5 cut(s) 515, 601, 836, 851, 998
Alw26I GTCTC 2 cut(s) 580, 977
AoxI GGCC 2 cut(s) 281, 701
ApaI GGGCCC 1 cut(s) 705
ApeKI GCWGC 1 cut(s) 598
ApoI RAATTY 6 cut(s) 356, 400, 913, 1039, 1117, 1234
AspLEI GCGC 4 cut(s) 598, 801, 950, 1257
AspS9I GGNCC 3 cut(s) 701, 702, 731
AsuHPI GGTGA 1 cut(s) 115
AvaII GGWCC 1 cut(s) 731
AxyI CCTNAGG 1 cut(s) 441
BaeGI GKGCMC 1 cut(s) 705
BanII GRGCYC 1 cut(s) 705
BbsI GAAGAC 1 cut(s) 380
BbvI GCAGC 1 cut(s) 610
BccI CCATC 1 cut(s) 692
BceAI ACGGC 1 cut(s) 734
BciT130I CCWGG 1 cut(s) 34
BcoDI GTCTC 2 cut(s) 580, 977
BfoI RGCGCY 1 cut(s) 802
BisI GCNGC 3 cut(s) 153, 203, 599
BlsI GCNGC 3 cut(s) 154, 204, 600
BmcAI AGTACT 1 cut(s) 641
Bme1390I CCNGG 1 cut(s) 34
Bme18I GGWCC 1 cut(s) 731
BmgT120I GGNCC 3 cut(s) 701, 702, 731
BmiI GGNNCC 3 cut(s) 654, 703, 733
BmrFI CCNGG 1 cut(s) 34
BmsI GCATC 2 cut(s) 307, 1021
BoxI GACNNNNGTC 1 cut(s) 632
BpiI GAAGAC 1 cut(s) 380
BplI GAGNNNNNCTC 2 cut(s) 101, 133
BpmI CTGGAG 1 cut(s) 401
BpuEI CTTGAG 1 cut(s) 141
BsaAI YACGTR 2 cut(s) 63, 542
BsaHI GRCGYC 1 cut(s) 90
BsaJI CCNNGG 3 cut(s) 33, 705, 1171
BsaXI ACNNNNNCTCC 4 cut(s) 158, 188, 242, 272
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 937
Bse1I ACTGG 4 cut(s) 366, 384, 619, 1280
Bse21I CCTNAGG 1 cut(s) 441
BseBI CCWGG 1 cut(s) 34
BseDI CCNNGG 3 cut(s) 33, 705, 1171
BseGI GGATG 1 cut(s) 1036
BseLI CCNNNNNNNGG 2 cut(s) 148, 937
BseMII CTCAG 2 cut(s) 455, 861
BseNI ACTGG 4 cut(s) 366, 384, 619, 1280
BseRI GAGGAG 5 cut(s) 94, 179, 263, 595, 598
BseSI GKGCMC 1 cut(s) 705
BseXI GCAGC 1 cut(s) 610
BseYI CCCAGC 2 cut(s) 931, 998
BsgI GTGCAG 1 cut(s) 1168
Bsh1236I CGCG 1 cut(s) 47
BshFI GGCC 2 cut(s) 283, 703
BsiSI CCGG 1 cut(s) 149
BslFI GGGAC 1 cut(s) 1232
BslI CCNNNNNNNGG 2 cut(s) 148, 937
BsmAI GTCTC 2 cut(s) 580, 977
BsmBI CGTCTC 1 cut(s) 977
BsmFI GGGAC 1 cut(s) 1232
BsnI GGCC 2 cut(s) 283, 703
Bsp120I GGGCCC 1 cut(s) 701
Bsp1286I GDGCHC 1 cut(s) 705
Bsp1407I TGTACA 1 cut(s) 1069
Bsp143I GATC 1 cut(s) 340
Bsp19I CCATGG 1 cut(s) 705
BspACI CCGC 9 cut(s) 75, 126, 152, 200, 203, 349, 475, 944, 1277
BspANI GGCC 2 cut(s) 283, 703
BspCNI CTCAG 2 cut(s) 454, 862
BspFNI CGCG 1 cut(s) 47
BspHI TCATGA 1 cut(s) 1009
BspLI GGNNCC 3 cut(s) 654, 703, 733
BspQI GCTCTTC 1 cut(s) 705
BsrBI CCGCTC 1 cut(s) 77
BsrGI TGTACA 1 cut(s) 1069
BsrI ACTGG 4 cut(s) 366, 384, 619, 1280
BssECI CCNNGG 3 cut(s) 33, 705, 1171
BssMI GATC 1 cut(s) 340
BssNI GRCGYC 1 cut(s) 90
BssT1I CCWWGG 2 cut(s) 705, 1171
Bst2UI CCWGG 1 cut(s) 34
Bst4CI ACNGT 4 cut(s) 258, 334, 424, 591
Bst6I CTCTTC 2 cut(s) 297, 705
BstACI GRCGYC 1 cut(s) 90
BstAPI GCANNNNNTGC 1 cut(s) 941
BstAUI TGTACA 1 cut(s) 1069
BstBAI YACGTR 2 cut(s) 63, 542
BstC8I GCNNGC 3 cut(s) 97, 501, 946
BstDEI CTNAG 2 cut(s) 441, 870
BstDSI CCRYGG 1 cut(s) 705
BstF5I GGATG 1 cut(s) 1036
BstFNI CGCG 1 cut(s) 47
BstH2I RGCGCY 1 cut(s) 802
BstHHI GCGC 4 cut(s) 598, 801, 950, 1257
BstKTI GATC 1 cut(s) 343
BstMAI GTCTC 2 cut(s) 580, 977
BstMBI GATC 1 cut(s) 340
BstMWI GCNNNNNNNGC 4 cut(s) 44, 326, 941, 1031
BstNI CCWGG 1 cut(s) 34
BstNSI RCATGY 2 cut(s) 226, 1191
BstPAI GACNNNNGTC 1 cut(s) 632
BstSCI CCNGG 1 cut(s) 32
BstSLI GKGCMC 1 cut(s) 705
BstUI CGCG 1 cut(s) 47
BstV1I GCAGC 1 cut(s) 610
BstV2I GAAGAC 1 cut(s) 380
Bsu36I CCTNAGG 1 cut(s) 441
BsuRI GGCC 2 cut(s) 283, 703
BtgI CCRYGG 1 cut(s) 705
BtsCI GGATG 1 cut(s) 1036
BtsIMutI CAGTG 3 cut(s) 377, 867, 904
Cac8I GCNNGC 3 cut(s) 97, 501, 946
CciI TCATGA 1 cut(s) 1009
CfoI GCGC 4 cut(s) 598, 801, 950, 1257
Cfr13I GGNCC 3 cut(s) 701, 702, 731
CseI GACGC 3 cut(s) 36, 637, 1164
Csp6I GTAC 5 cut(s) 23, 64, 640, 1070, 1111
CspCI CAANNNNNGTGG 2 cut(s) 856, 891
CviAII CATG 4 cut(s) 223, 706, 1010, 1188
CviQI GTAC 5 cut(s) 23, 64, 640, 1070, 1111
DdeI CTNAG 2 cut(s) 441, 870
DpnI GATC 1 cut(s) 342
DpnII GATC 1 cut(s) 340
DrdI GACNNNNNNGTC 1 cut(s) 382
DseDI GACNNNNNNGTC 1 cut(s) 382
Eam1104I CTCTTC 2 cut(s) 297, 705
EarI CTCTTC 2 cut(s) 297, 705
EciI GGCGGA 1 cut(s) 338
Eco130I CCWWGG 2 cut(s) 705, 1171
Eco24I GRGCYC 1 cut(s) 705
Eco47I GGWCC 1 cut(s) 731
Eco81I CCTNAGG 1 cut(s) 441
EcoRII CCWGG 1 cut(s) 32
EcoT14I CCWWGG 2 cut(s) 705, 1171
EcoT38I GRGCYC 1 cut(s) 705
ErhI CCWWGG 2 cut(s) 705, 1171
Esp3I CGTCTC 1 cut(s) 977
FaeI CATG 4 cut(s) 226, 709, 1013, 1191
FalI AAGNNNNNCTT 2 cut(s) 1070, 1102
FaqI GGGAC 1 cut(s) 1232
FatI CATG 4 cut(s) 222, 705, 1009, 1187
FauI CCCGC 3 cut(s) 82, 468, 937
FauNDI CATATG 1 cut(s) 243
FblI GTMKAC 3 cut(s) 213, 621, 861
Fnu4HI GCNGC 3 cut(s) 153, 203, 599
FokI GGATG 1 cut(s) 1043
FriOI GRGCYC 1 cut(s) 705
Fsp4HI GCNGC 3 cut(s) 153, 203, 599
GlaI GCGC 4 cut(s) 597, 800, 949, 1256
GluI GCNGC 3 cut(s) 153, 203, 599
GsaI CCCAGC 2 cut(s) 935, 1002
GsuI CTGGAG 1 cut(s) 401
HaeII RGCGCY 1 cut(s) 802
HaeIII GGCC 2 cut(s) 283, 703
HapII CCGG 1 cut(s) 149
HgaI GACGC 3 cut(s) 36, 637, 1164
HhaI GCGC 4 cut(s) 598, 801, 950, 1257
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 4 cut(s) 226, 709, 1013, 1191
Hin6I GCGC 4 cut(s) 596, 799, 948, 1255
HinP1I GCGC 4 cut(s) 596, 799, 948, 1255
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 1 cut(s) 513
HinfI GANTC 9 cut(s) 112, 232, 307, 383, 565, 774, 1163, 1200, 1289
HpaII CCGG 1 cut(s) 149
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 6 cut(s) 197, 214, 545, 622, 862, 1113
Hpy188I TCNGA 5 cut(s) 109, 406, 467, 487, 1168
Hpy188III TCNNGA 5 cut(s) 236, 296, 311, 443, 1010
Hpy8I GTNNAC 6 cut(s) 197, 214, 545, 622, 862, 1113
Hpy99I CGWCG 4 cut(s) 92, 95, 218, 1158
HpyAV CCTTC 4 cut(s) 130, 950, 954, 1240
HpyCH4III ACNGT 4 cut(s) 258, 334, 424, 591
HpyCH4IV ACGT 7 cut(s) 62, 90, 409, 541, 547, 724, 890
HpyCH4V TGCA 6 cut(s) 611, 911, 1185, 1272, 1286, 1297
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 326, 941, 1031
HpyF3I CTNAG 2 cut(s) 441, 870
HpySE526I ACGT 7 cut(s) 62, 90, 409, 541, 547, 724, 890
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 4 cut(s) 226, 709, 1013, 1191
HspAI GCGC 4 cut(s) 596, 799, 948, 1255
Kzo9I GATC 1 cut(s) 340
LguI GCTCTTC 1 cut(s) 705
LmnI GCTCC 3 cut(s) 122, 141, 478
Lsp1109I GCAGC 1 cut(s) 610
LweI GCATC 2 cut(s) 307, 1021
MaeII ACGT 7 cut(s) 62, 90, 409, 541, 547, 724, 890
MalI GATC 1 cut(s) 342
MbiI CCGCTC 1 cut(s) 77
MboI GATC 1 cut(s) 340
MboII GAAGA 7 cut(s) 241, 284, 385, 722, 857, 1090, 1113
MhlI GDGCHC 1 cut(s) 705
MlyI GAGTC 4 cut(s) 316, 392, 768, 1298
MseI TTAA 2 cut(s) 818, 1242
MslI CAYNNNNRTG 1 cut(s) 425
MspA1I CMGCKG 2 cut(s) 475, 998
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 1 cut(s) 34
MvaI CCWGG 1 cut(s) 34
MvnI CGCG 1 cut(s) 47
MwoI GCNNNNNNNGC 4 cut(s) 44, 326, 941, 1031
NcoI CCATGG 1 cut(s) 705
NdeI CATATG 1 cut(s) 243
NdeII GATC 1 cut(s) 340
NlaIII CATG 4 cut(s) 226, 709, 1013, 1191
NlaIV GGNNCC 3 cut(s) 654, 703, 733
NspI RCATGY 2 cut(s) 226, 1191
PagI TCATGA 1 cut(s) 1009
PciI ACATGT 1 cut(s) 222
PciSI GCTCTTC 1 cut(s) 705
PfeI GAWTC 5 cut(s) 112, 232, 565, 1163, 1200
PkrI GCNGC 3 cut(s) 154, 204, 600
PleI GAGTC 4 cut(s) 315, 391, 768, 1297
PpsI GAGTC 4 cut(s) 315, 391, 768, 1297
Ppu21I YACGTR 2 cut(s) 63, 542
PscI ACATGT 1 cut(s) 222
PshAI GACNNNNGTC 1 cut(s) 632
Psp1406I AACGTT 2 cut(s) 547, 724
Psp6I CCWGG 1 cut(s) 32
PspFI CCCAGC 2 cut(s) 931, 998
PspGI CCWGG 1 cut(s) 32
PspN4I GGNNCC 3 cut(s) 654, 703, 733
PspOMI GGGCCC 1 cut(s) 701
PspPI GGNCC 3 cut(s) 701, 702, 731
PvuII CAGCTG 1 cut(s) 998
RsaI GTAC 5 cut(s) 24, 65, 641, 1071, 1112
RsaNI GTAC 5 cut(s) 23, 64, 640, 1070, 1111
RseI CAYNNNNRTG 1 cut(s) 425
SalI GTCGAC 1 cut(s) 212
SapI GCTCTTC 1 cut(s) 705
SaqAI TTAA 2 cut(s) 818, 1242
SatI GCNGC 3 cut(s) 153, 203, 599
Sau3AI GATC 1 cut(s) 340
Sau96I GGNCC 3 cut(s) 701, 702, 731
ScaI AGTACT 1 cut(s) 641
SchI GAGTC 4 cut(s) 316, 392, 768, 1298
ScrFI CCNGG 1 cut(s) 34
SduI GDGCHC 1 cut(s) 705
SfaNI GCATC 2 cut(s) 307, 1021
SinI GGWCC 1 cut(s) 731
SmiMI CAYNNNNRTG 1 cut(s) 425
SmlI CTYRAG 1 cut(s) 156
SmoI CTYRAG 1 cut(s) 156
SsiI CCGC 9 cut(s) 75, 126, 152, 200, 203, 349, 475, 944, 1277
StyD4I CCNGG 1 cut(s) 32
StyI CCWWGG 2 cut(s) 705, 1171
TaaI ACNGT 4 cut(s) 258, 334, 424, 591
TaiI ACGT 7 cut(s) 65, 93, 412, 544, 550, 727, 893
TaqI TCGA 5 cut(s) 213, 235, 295, 305, 1107
TatI WGTACW 3 cut(s) 639, 1069, 1110
TauI GCSGC 2 cut(s) 155, 205
TfiI GAWTC 5 cut(s) 112, 232, 565, 1163, 1200
Tru1I TTAA 2 cut(s) 818, 1242
Tru9I TTAA 2 cut(s) 818, 1242
TscAI CASTG 3 cut(s) 384, 874, 904
TseI GCWGC 1 cut(s) 598
TspDTI ATGAA 2 cut(s) 883, 1032
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 3 cut(s) 384, 874, 904
VpaK11BI GGWCC 1 cut(s) 731
XapI RAATTY 6 cut(s) 356, 400, 913, 1039, 1117, 1234
XceI RCATGY 2 cut(s) 226, 1191
XmiI GTMKAC 3 cut(s) 213, 621, 861
ZraI GACGTC 1 cut(s) 91
ZrmI AGTACT 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.