pycom04g16010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001309
Physical Location & Seq
Forward (+)
18721490 .. 18725365
3876 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g16010.5

Sequence Viewer

Length: 1323 bp
ATGGCTTCCACGGAGGGTATAGTACCAATAACCAGGGCTTTTCTCGCTTCCTACTACGACACATACCAATTTCCCCCTCTCTCAAACGACGTCGCTCGCCTCTCAGCCGAGATTCGATCATTCACCGCCGATTTGCTCGGCCACTCTCCGGCGGCTCAAGGCGAGGAGAGATTGTTAGTGAGTGAATTGGAGAGTGAACCTCCCCATAAAGTTGACGAAAACATGTGGAAGAATCGAGAGCATATGGAAGAGATACTATTTTTGCTTGAAAAATCCCATTGGCCTCAAACTCTTCGAAAGCAGTCCAGTCCTGATGATGCTGAACTGGCTACTGTGATTGATCGTCTCCACCAGAAATTTGAAACCAGTTTGAAGACATTGGAGACTTTCCAAGCGAAAAATTCTGAACGTGTGTTCAACACGGTTATGACTTACATTCCCCAGGATTTTCGGGGAAGGCTCATCAGACTACAGCGGGAGCGTTCAGAGAGGAATAAGCAAGCAGAGGTTGAAGCTTTGGTTAGTTCTGGAGCAACTATACGTGAACGTTATGCTCTCTTGTGGAACCAACAAATGGAGAGGAGGAGACTGTTAGCGCAGCTTGGTTCTGCAACTGGTGTCTACAAGACGCTTGTCAAGTACTTGGTTGGGGTTCCACAGGTTTTACTTGATTTTATTCGCCAAATAAATGATGATGATGGGCCCATGGAAGAGCAACGACAGCGTTATGGACCACCATTATACAGCCTTACAACTATGTCGCTTCTTATACGACTCTTTATTTCGTTGTCTTGGGGGCGATTTGAGACTAGAAAATTAAGCAAGCAAGAGTTAACTGTCTTCGAAGAAGCTGTTGATGTCTACACCACTGAATTTGTAAGGTTTATTACGTTCATCAGTGAAGTCTTTGCAAATTCCCCCTTTTTTATCCCAGCAGAGGTTGCGGGCGCATTACAAGGAAGGAATAACGATGATTACCAAGAGACCAGTGTTCCAGCTGGGAAAATTCATGAGGTTTCATTGGCTGTGGACGCAATAAATTCATATATTGCTTGGGATTTCTCGCTTGTACAAGGGAAGATAAACTTGGATATTGGATTTAGTGTTGAGTATACAAATCCATCTGGGCAAAAATCTCTCATTTTACCTTACCGACGTTTCGAGTCTGACCAAGGAAACTTCTGCACATGCGAGGTGGGAAACTACAAACTGATTTGGGACAATTCCTATTCTAGTTTTTTTAAGAAGGTGCTGCGCTACAAGGTAGATTGCATACCACCAGTCACAGAGCCTGTTGAACCTGCGACTGAAGTGGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

441

Amino Acids

50.55

Weight (kDa)

5.2

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 93
Acc36I ACCTGC 1 cut(s) 1309
AccB7I CCANNNNNTGG 1 cut(s) 574
AccI GTMKAC 3 cut(s) 621, 861, 1112
AciI CCGC 4 cut(s) 126, 152, 475, 944
AclI AACGTT 1 cut(s) 547
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 6 cut(s) 356, 400, 872, 913, 1005, 1039
AcyI GRCGYC 1 cut(s) 90
AfaI GTAC 3 cut(s) 24, 641, 1071
AfiI CCNNNNNNNGG 3 cut(s) 148, 574, 937
AflIII ACRYGT 2 cut(s) 222, 409
AgsI TTSAA 6 cut(s) 269, 362, 373, 418, 512, 1298
AjnI CCWGG 2 cut(s) 32, 441
AjuI GAANNNNNNNTTGG 2 cut(s) 1070, 1102
AluBI AGCT 4 cut(s) 515, 601, 851, 998
AluI AGCT 4 cut(s) 515, 601, 851, 998
Alw26I GTCTC 5 cut(s) 350, 377, 580, 800, 977
AlwNI CAGNNNCTG 1 cut(s) 1292
AoxI GGCC 3 cut(s) 139, 281, 701
ApaI GGGCCC 1 cut(s) 705
ApeKI GCWGC 2 cut(s) 598, 1252
ApoI RAATTY 6 cut(s) 356, 400, 872, 913, 1005, 1039
AspLEI GCGC 3 cut(s) 598, 950, 1257
AspS9I GGNCC 3 cut(s) 701, 702, 731
AsuHPI GGTGA 1 cut(s) 115
AsuII TTCGAA 2 cut(s) 295, 843
AvaII GGWCC 1 cut(s) 731
BaeGI GKGCMC 1 cut(s) 705
BanII GRGCYC 1 cut(s) 705
BbsI GAAGAC 2 cut(s) 380, 832
BbvI GCAGC 2 cut(s) 610, 1239
BccI CCATC 2 cut(s) 692, 1129
BciT130I CCWGG 2 cut(s) 34, 443
BcoDI GTCTC 5 cut(s) 350, 377, 580, 800, 977
BfaI CTAG 2 cut(s) 810, 1233
BfmI CTRYAG 1 cut(s) 470
BfuAI ACCTGC 1 cut(s) 1309
BisI GCNGC 3 cut(s) 153, 599, 1253
BlsI GCNGC 3 cut(s) 154, 600, 1254
BmcAI AGTACT 1 cut(s) 641
Bme1390I CCNGG 2 cut(s) 34, 443
Bme18I GGWCC 1 cut(s) 731
BmgT120I GGNCC 3 cut(s) 701, 702, 731
BmiI GGNNCC 3 cut(s) 566, 654, 703
BmrFI CCNGG 2 cut(s) 34, 443
BmsI GCATC 1 cut(s) 307
BoxI GACNNNNGTC 1 cut(s) 632
BpiI GAAGAC 2 cut(s) 380, 832
BplI GAGNNNNNCTC 2 cut(s) 184, 216
BpmI CTGGAG 1 cut(s) 549
Bpu14I TTCGAA 2 cut(s) 295, 843
BpuEI CTTGAG 1 cut(s) 141
BsaAI YACGTR 1 cut(s) 542
BsaHI GRCGYC 1 cut(s) 90
BsaI GGTCTC 1 cut(s) 977
BsaJI CCNNGG 5 cut(s) 9, 33, 441, 705, 1171
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 574, 937
Bse1I ACTGG 6 cut(s) 306, 330, 366, 619, 987, 1280
BseBI CCWGG 2 cut(s) 34, 443
BseDI CCNNGG 5 cut(s) 9, 33, 441, 705, 1171
BseLI CCNNNNNNNGG 3 cut(s) 148, 574, 937
BseMII CTCAG 1 cut(s) 117
BseNI ACTGG 6 cut(s) 306, 330, 366, 619, 987, 1280
BseRI GAGGAG 3 cut(s) 179, 595, 598
BseSI GKGCMC 1 cut(s) 705
BseXI GCAGC 2 cut(s) 610, 1239
BseYI CCCAGC 2 cut(s) 931, 998
BsgI GTGCAG 1 cut(s) 1168
BshFI GGCC 3 cut(s) 141, 283, 703
BsiSI CCGG 1 cut(s) 149
BslFI GGGAC 1 cut(s) 1232
BslI CCNNNNNNNGG 3 cut(s) 148, 574, 937
BsmAI GTCTC 5 cut(s) 350, 377, 580, 800, 977
BsmBI CGTCTC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 1232
BsnI GGCC 3 cut(s) 141, 283, 703
Bso31I GGTCTC 1 cut(s) 977
Bsp119I TTCGAA 2 cut(s) 295, 843
Bsp120I GGGCCC 1 cut(s) 701
Bsp1286I GDGCHC 1 cut(s) 705
Bsp1407I TGTACA 1 cut(s) 1069
Bsp143I GATC 2 cut(s) 116, 340
Bsp19I CCATGG 1 cut(s) 705
BspACI CCGC 4 cut(s) 126, 152, 475, 944
BspANI GGCC 3 cut(s) 141, 283, 703
BspCNI CTCAG 1 cut(s) 116
BspHI TCATGA 1 cut(s) 1009
BspLI GGNNCC 3 cut(s) 566, 654, 703
BspMI ACCTGC 1 cut(s) 1309
BspQI GCTCTTC 1 cut(s) 705
BspT104I TTCGAA 2 cut(s) 295, 843
BspTNI GGTCTC 1 cut(s) 977
BsrGI TGTACA 1 cut(s) 1069
BsrI ACTGG 6 cut(s) 306, 330, 366, 619, 987, 1280
BssECI CCNNGG 5 cut(s) 9, 33, 441, 705, 1171
BssMI GATC 2 cut(s) 116, 340
BssNAI GTATAC 1 cut(s) 1113
BssNI GRCGYC 1 cut(s) 90
BssT1I CCWWGG 2 cut(s) 705, 1171
Bst1107I GTATAC 1 cut(s) 1113
Bst2UI CCWGG 2 cut(s) 34, 443
Bst4CI ACNGT 4 cut(s) 334, 424, 591, 838
Bst6I CTCTTC 3 cut(s) 243, 297, 705
BstACI GRCGYC 1 cut(s) 90
BstAPI GCANNNNNTGC 1 cut(s) 941
BstAUI TGTACA 1 cut(s) 1069
BstBAI YACGTR 1 cut(s) 542
BstBI TTCGAA 2 cut(s) 295, 843
BstC8I GCNNGC 4 cut(s) 97, 501, 824, 946
BstDEI CTNAG 1 cut(s) 103
BstDSI CCRYGG 2 cut(s) 9, 705
BstHHI GCGC 3 cut(s) 598, 950, 1257
BstKTI GATC 2 cut(s) 119, 343
BstMAI GTCTC 5 cut(s) 350, 377, 580, 800, 977
BstMBI GATC 2 cut(s) 116, 340
BstMWI GCNNNNNNNGC 5 cut(s) 44, 326, 721, 941, 1031
BstNI CCWGG 2 cut(s) 34, 443
BstNSI RCATGY 2 cut(s) 226, 1191
BstPAI GACNNNNGTC 1 cut(s) 632
BstSCI CCNGG 2 cut(s) 32, 441
BstSFI CTRYAG 1 cut(s) 470
BstSLI GKGCMC 1 cut(s) 705
BstV1I GCAGC 2 cut(s) 610, 1239
BstV2I GAAGAC 2 cut(s) 380, 832
BstZ17I GTATAC 1 cut(s) 1113
BsuRI GGCC 3 cut(s) 141, 283, 703
BtgI CCRYGG 2 cut(s) 9, 705
BtsIMutI CAGTG 3 cut(s) 867, 904, 994
BveI ACCTGC 1 cut(s) 1309
Cac8I GCNNGC 4 cut(s) 97, 501, 824, 946
CaiI CAGNNNCTG 1 cut(s) 1292
CciI TCATGA 1 cut(s) 1009
CfoI GCGC 3 cut(s) 598, 950, 1257
Cfr13I GGNCC 3 cut(s) 701, 702, 731
CseI GACGC 2 cut(s) 637, 1040
Csp6I GTAC 3 cut(s) 23, 640, 1070
CspCI CAANNNNNGTGG 2 cut(s) 856, 891
CviAII CATG 4 cut(s) 223, 706, 1010, 1188
CviQI GTAC 3 cut(s) 23, 640, 1070
DdeI CTNAG 1 cut(s) 103
DpnI GATC 2 cut(s) 118, 342
DpnII GATC 2 cut(s) 116, 340
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 3 cut(s) 243, 297, 705
EarI CTCTTC 3 cut(s) 243, 297, 705
Eco130I CCWWGG 2 cut(s) 705, 1171
Eco24I GRGCYC 1 cut(s) 705
Eco31I GGTCTC 1 cut(s) 977
Eco47I GGWCC 1 cut(s) 731
EcoRII CCWGG 2 cut(s) 32, 441
EcoT14I CCWWGG 2 cut(s) 705, 1171
EcoT38I GRGCYC 1 cut(s) 705
ErhI CCWWGG 2 cut(s) 705, 1171
Esp3I CGTCTC 1 cut(s) 350
FaeI CATG 4 cut(s) 226, 709, 1013, 1191
FalI AAGNNNNNCTT 2 cut(s) 1070, 1102
FaqI GGGAC 1 cut(s) 1232
FatI CATG 4 cut(s) 222, 705, 1009, 1187
FauI CCCGC 2 cut(s) 468, 937
FauNDI CATATG 1 cut(s) 243
FblI GTMKAC 3 cut(s) 621, 861, 1112
Fnu4HI GCNGC 3 cut(s) 153, 599, 1253
FriOI GRGCYC 1 cut(s) 705
Fsp4HI GCNGC 3 cut(s) 153, 599, 1253
FspBI CTAG 2 cut(s) 810, 1233
GlaI GCGC 3 cut(s) 597, 949, 1256
GluI GCNGC 3 cut(s) 153, 599, 1253
GsaI CCCAGC 2 cut(s) 935, 1002
GsuI CTGGAG 1 cut(s) 549
HaeIII GGCC 3 cut(s) 141, 283, 703
HapII CCGG 1 cut(s) 149
HgaI GACGC 2 cut(s) 637, 1040
HhaI GCGC 3 cut(s) 598, 950, 1257
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 4 cut(s) 226, 709, 1013, 1191
Hin6I GCGC 3 cut(s) 596, 948, 1255
HinP1I GCGC 3 cut(s) 596, 948, 1255
HincII GTYRAC 2 cut(s) 214, 834
HindII GTYRAC 2 cut(s) 214, 834
HindIII AAGCTT 1 cut(s) 513
HinfI GANTC 4 cut(s) 112, 232, 774, 1163
HpaI GTTAAC 1 cut(s) 834
HpaII CCGG 1 cut(s) 149
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 8 cut(s) 197, 214, 545, 622, 834, 862, 1030, 1113
Hpy188I TCNGA 4 cut(s) 406, 467, 487, 1168
Hpy188III TCNNGA 4 cut(s) 236, 311, 528, 1010
Hpy8I GTNNAC 8 cut(s) 197, 214, 545, 622, 834, 862, 1030, 1113
Hpy99I CGWCG 3 cut(s) 92, 95, 1158
HpyAV CCTTC 3 cut(s) 450, 954, 1240
HpyCH4III ACNGT 4 cut(s) 334, 424, 591, 838
HpyCH4IV ACGT 6 cut(s) 90, 409, 541, 547, 890, 1156
HpyCH4V TGCA 4 cut(s) 611, 911, 1185, 1272
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 326, 721, 941, 1031
HpyF3I CTNAG 1 cut(s) 103
HpySE526I ACGT 6 cut(s) 90, 409, 541, 547, 890, 1156
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 4 cut(s) 226, 709, 1013, 1191
HspAI GCGC 3 cut(s) 596, 948, 1255
KspAI GTTAAC 1 cut(s) 834
Kzo9I GATC 2 cut(s) 116, 340
LguI GCTCTTC 1 cut(s) 705
LmnI GCTCC 2 cut(s) 478, 530
Lsp1109I GCAGC 2 cut(s) 610, 1239
LweI GCATC 1 cut(s) 307
MaeI CTAG 2 cut(s) 810, 1233
MaeII ACGT 6 cut(s) 90, 409, 541, 547, 890, 1156
MaeIII GTNAC 1 cut(s) 1282
MalI GATC 2 cut(s) 118, 342
MboI GATC 2 cut(s) 116, 340
MboII GAAGA 8 cut(s) 241, 260, 284, 385, 722, 832, 857, 1090
MhlI GDGCHC 1 cut(s) 705
MlyI GAGTC 2 cut(s) 768, 1172
MseI TTAA 3 cut(s) 818, 833, 1242
MslI CAYNNNNRTG 1 cut(s) 425
MspA1I CMGCKG 2 cut(s) 475, 998
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 2 cut(s) 34, 443
MvaI CCWGG 2 cut(s) 34, 443
MwoI GCNNNNNNNGC 5 cut(s) 44, 326, 721, 941, 1031
NcoI CCATGG 1 cut(s) 705
NdeI CATATG 1 cut(s) 243
NdeII GATC 2 cut(s) 116, 340
NlaIII CATG 4 cut(s) 226, 709, 1013, 1191
NlaIV GGNNCC 3 cut(s) 566, 654, 703
NmeAIII GCCGAG 2 cut(s) 117, 133
NmuCI GTSAC 1 cut(s) 1282
NspI RCATGY 2 cut(s) 226, 1191
NspV TTCGAA 2 cut(s) 295, 843
PagI TCATGA 1 cut(s) 1009
PciI ACATGT 1 cut(s) 222
PciSI GCTCTTC 1 cut(s) 705
PfeI GAWTC 2 cut(s) 112, 232
PflMI CCANNNNNTGG 1 cut(s) 574
PkrI GCNGC 3 cut(s) 154, 600, 1254
PleI GAGTC 2 cut(s) 768, 1171
PpsI GAGTC 2 cut(s) 768, 1171
Ppu21I YACGTR 1 cut(s) 542
PscI ACATGT 1 cut(s) 222
PshAI GACNNNNGTC 1 cut(s) 632
Psp1406I AACGTT 1 cut(s) 547
Psp6I CCWGG 2 cut(s) 32, 441
PspFI CCCAGC 2 cut(s) 931, 998
PspGI CCWGG 2 cut(s) 32, 441
PspN4I GGNNCC 3 cut(s) 566, 654, 703
PspOMI GGGCCC 1 cut(s) 701
PspPI GGNCC 3 cut(s) 701, 702, 731
PstNI CAGNNNCTG 1 cut(s) 1292
PvuII CAGCTG 1 cut(s) 998
RsaI GTAC 3 cut(s) 24, 641, 1071
RsaNI GTAC 3 cut(s) 23, 640, 1070
RseI CAYNNNNRTG 1 cut(s) 425
SapI GCTCTTC 1 cut(s) 705
SaqAI TTAA 3 cut(s) 818, 833, 1242
SatI GCNGC 3 cut(s) 153, 599, 1253
Sau3AI GATC 2 cut(s) 116, 340
Sau96I GGNCC 3 cut(s) 701, 702, 731
ScaI AGTACT 1 cut(s) 641
SchI GAGTC 2 cut(s) 768, 1172
ScrFI CCNGG 2 cut(s) 34, 443
SduI GDGCHC 1 cut(s) 705
SfaNI GCATC 1 cut(s) 307
SfcI CTRYAG 1 cut(s) 470
SfuI TTCGAA 2 cut(s) 295, 843
SinI GGWCC 1 cut(s) 731
SmiMI CAYNNNNRTG 1 cut(s) 425
SmlI CTYRAG 1 cut(s) 156
SmoI CTYRAG 1 cut(s) 156
SsiI CCGC 4 cut(s) 126, 152, 475, 944
SspMI CTAG 2 cut(s) 810, 1233
StyD4I CCNGG 2 cut(s) 32, 441
StyI CCWWGG 2 cut(s) 705, 1171
TaaI ACNGT 4 cut(s) 334, 424, 591, 838
TaiI ACGT 6 cut(s) 93, 412, 544, 550, 893, 1159
TaqI TCGA 5 cut(s) 115, 235, 295, 843, 1161
TatI WGTACW 2 cut(s) 639, 1069
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 2 cut(s) 112, 232
Tru1I TTAA 3 cut(s) 818, 833, 1242
Tru9I TTAA 3 cut(s) 818, 833, 1242
TscAI CASTG 3 cut(s) 874, 904, 994
TseFI GTSAC 1 cut(s) 1282
TseI GCWGC 2 cut(s) 598, 1252
Tsp45I GTSAC 1 cut(s) 1282
TspDTI ATGAA 4 cut(s) 883, 998, 1008, 1032
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 3 cut(s) 874, 904, 994
Van91I CCANNNNNTGG 1 cut(s) 574
VpaK11BI GGWCC 1 cut(s) 731
XapI RAATTY 6 cut(s) 356, 400, 872, 913, 1005, 1039
XceI RCATGY 2 cut(s) 226, 1191
XmiI GTMKAC 3 cut(s) 621, 861, 1112
XspI CTAG 2 cut(s) 810, 1233
ZraI GACGTC 1 cut(s) 91
ZrmI AGTACT 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.