RchiOBHm_Chr3g0494831

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001309
Physical Location & Seq
Forward (+)
43807183 .. 43807726
544 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45733

Sequence Viewer

Length: 447 bp
ATGGCTTCTTCCACGGAGAGTCTGGTGCCCATGACCAGGGCTTTTCTCACTTCTTATTATGACACCTACAAATTCCTTCCTCTCTCCGATGACATCCCCCGCCTCTCCTCTGAGATTCGATCCCTTACCGCCGATTTGCTCTCCAACTCTTCGGCCACTCCAGGAGAAGAGAGATTAGTAGTGGATGAACTAGAGAGTCCTCCGCCGCATAAAGTGGATGAGAGTATGTGGAAGAATCGGGAGCATATGGAAGAGGTAGTCCTTTTACTTGATAGGCCTCATTGGCCTCAATCGGTGAGCATTGATGTGAATGTTAAACAGCTGGTGTTACCTTACATATTTTTGTTTCTTATTTTTGGTGGTATGAGCAGCTTTAAGAGCCTTCGAGTCCCGAGGATGCTCAGCTTGCTATTGTTTTCATTTGCCTGCAACATAAGTTTAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

16.85

Weight (kDa)

5.13

Isoelectric Point (pI)

55.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 25
AciI CCGC 4 cut(s) 100, 129, 203, 206
AclWI GGATC 1 cut(s) 114
AcoI YGGCCR 1 cut(s) 153
AcsI RAATTY 1 cut(s) 71
AfiI CCNNNNNNNGG 1 cut(s) 36
AjnI CCWGG 2 cut(s) 35, 160
AluBI AGCT 3 cut(s) 322, 372, 405
AluI AGCT 3 cut(s) 322, 372, 405
AlwI GGATC 1 cut(s) 114
Ama87I CYCGRG 1 cut(s) 391
AoxI GGCC 3 cut(s) 153, 275, 284
ApeKI GCWGC 1 cut(s) 369
ApoI RAATTY 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 307
AvaI CYCGRG 1 cut(s) 391
BaeGI GKGCMC 1 cut(s) 30
BanI GGYRCC 1 cut(s) 25
BbvI GCAGC 1 cut(s) 381
BciT130I CCWGG 2 cut(s) 37, 162
BfaI CTAG 1 cut(s) 191
BglI GCCNNNNNGGC 1 cut(s) 283
BisI GCNGC 2 cut(s) 206, 370
BlpI GCTNAGC 1 cut(s) 401
BlsI GCNGC 2 cut(s) 207, 371
Bme1390I CCNGG 2 cut(s) 37, 162
BmeT110I CYCGRG 1 cut(s) 391
BmiI GGNNCC 1 cut(s) 27
BmrFI CCNGG 2 cut(s) 37, 162
BmsI GCATC 1 cut(s) 387
BpmI CTGGAG 1 cut(s) 144
Bpu1102I GCTNAGC 1 cut(s) 401
BsaJI CCNNGG 3 cut(s) 12, 36, 392
Bsc4I CCNNNNNNNGG 1 cut(s) 36
BseBI CCWGG 2 cut(s) 37, 162
BseDI CCNNGG 3 cut(s) 12, 36, 392
BseGI GGATG 4 cut(s) 93, 190, 223, 402
BseLI CCNNNNNNNGG 1 cut(s) 36
BseMII CTCAG 2 cut(s) 102, 415
BseRI GAGGAG 1 cut(s) 97
BseSI GKGCMC 1 cut(s) 30
BseXI GCAGC 1 cut(s) 381
BshFI GGCC 3 cut(s) 155, 277, 286
BshNI GGYRCC 1 cut(s) 25
BsiHKCI CYCGRG 1 cut(s) 391
BslFI GGGAC 1 cut(s) 374
BslI CCNNNNNNNGG 1 cut(s) 36
BsmFI GGGAC 1 cut(s) 374
BsnI GGCC 3 cut(s) 155, 277, 286
BsoBI CYCGRG 1 cut(s) 391
Bsp1286I GDGCHC 1 cut(s) 30
Bsp143I GATC 1 cut(s) 119
Bsp1720I GCTNAGC 1 cut(s) 401
BspACI CCGC 4 cut(s) 100, 129, 203, 206
BspANI GGCC 3 cut(s) 155, 277, 286
BspCNI CTCAG 2 cut(s) 103, 414
BspLI GGNNCC 1 cut(s) 27
BspPI GGATC 1 cut(s) 114
BspT107I GGYRCC 1 cut(s) 25
BssECI CCNNGG 3 cut(s) 12, 36, 392
BssMI GATC 1 cut(s) 119
Bst2UI CCWGG 2 cut(s) 37, 162
Bst6I CTCTTC 3 cut(s) 154, 162, 246
BstC8I GCNNGC 2 cut(s) 407, 427
BstDEI CTNAG 2 cut(s) 111, 401
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 4 cut(s) 93, 190, 223, 402
BstKTI GATC 1 cut(s) 122
BstMBI GATC 1 cut(s) 119
BstMWI GCNNNNNNNGC 3 cut(s) 283, 378, 406
BstNI CCWGG 2 cut(s) 37, 162
BstSCI CCNGG 2 cut(s) 35, 160
BstSLI GKGCMC 1 cut(s) 30
BstV1I GCAGC 1 cut(s) 381
BsuRI GGCC 3 cut(s) 155, 277, 286
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 4 cut(s) 93, 190, 223, 402
Cac8I GCNNGC 2 cut(s) 407, 427
CviAII CATG 1 cut(s) 31
CviJI RGCY 9 cut(s) 5, 41, 155, 277, 286, 322, 372, 381, 405
CviKI_1 RGCY 9 cut(s) 5, 41, 155, 277, 286, 322, 372, 381, 405
DdeI CTNAG 2 cut(s) 111, 401
DpnI GATC 1 cut(s) 121
DpnII GATC 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 153
Eam1104I CTCTTC 3 cut(s) 154, 162, 246
EarI CTCTTC 3 cut(s) 154, 162, 246
EciI GGCGGA 1 cut(s) 192
Eco147I AGGCCT 1 cut(s) 277
Eco88I CYCGRG 1 cut(s) 391
EcoRII CCWGG 2 cut(s) 35, 160
FaeI CATG 1 cut(s) 34
FaqI GGGAC 1 cut(s) 374
FatI CATG 1 cut(s) 30
FauI CCCGC 1 cut(s) 107
FauNDI CATATG 1 cut(s) 246
Fnu4HI GCNGC 2 cut(s) 206, 370
FokI GGATG 4 cut(s) 80, 197, 230, 409
Fsp4HI GCNGC 2 cut(s) 206, 370
FspBI CTAG 1 cut(s) 191
GluI GCNGC 2 cut(s) 206, 370
GsuI CTGGAG 1 cut(s) 144
HaeIII GGCC 3 cut(s) 155, 277, 286
Hin1II CATG 1 cut(s) 34
HinfI GANTC 5 cut(s) 19, 115, 196, 235, 387
HphI GGTGA 1 cut(s) 307
Hpy188I TCNGA 2 cut(s) 88, 112
Hpy188III TCNNGA 2 cut(s) 239, 391
HpyAV CCTTC 2 cut(s) 86, 392
HpyCH4V TGCA 1 cut(s) 429
HpyF10VI GCNNNNNNNGC 3 cut(s) 283, 378, 406
HpyF3I CTNAG 2 cut(s) 111, 401
Hsp92II CATG 1 cut(s) 34
Kzo9I GATC 1 cut(s) 119
LmnI GCTCC 1 cut(s) 241
LpnPI CCDG 7 cut(s) 8, 22, 49, 147, 174, 308, 439
Lsp1109I GCAGC 1 cut(s) 381
LweI GCATC 1 cut(s) 387
MaeI CTAG 1 cut(s) 191
MaeIII GTNAC 1 cut(s) 327
MalI GATC 1 cut(s) 121
MboI GATC 1 cut(s) 119
MboII GAAGA 4 cut(s) 141, 179, 244, 263
MhlI GDGCHC 1 cut(s) 30
MluCI AATT 1 cut(s) 71
MlyI GAGTC 3 cut(s) 28, 205, 396
MmeI TCCRAC 1 cut(s) 168
MnlI CCTC 8 cut(s) 90, 113, 118, 210, 247, 288, 297, 387
MseI TTAA 3 cut(s) 315, 375, 440
MslI CAYNNNNRTG 1 cut(s) 305
MspA1I CMGCKG 1 cut(s) 322
MspR9I CCNGG 2 cut(s) 37, 162
MvaI CCWGG 2 cut(s) 37, 162
MwoI GCNNNNNNNGC 3 cut(s) 283, 378, 406
NdeI CATATG 1 cut(s) 246
NdeII GATC 1 cut(s) 119
NlaIII CATG 1 cut(s) 34
NlaIV GGNNCC 1 cut(s) 27
PceI AGGCCT 1 cut(s) 277
PfeI GAWTC 2 cut(s) 115, 235
PfoI TCCNGGA 1 cut(s) 160
PkrI GCNGC 2 cut(s) 207, 371
PleI GAGTC 3 cut(s) 27, 204, 395
PpsI GAGTC 3 cut(s) 27, 204, 395
Psp6I CCWGG 2 cut(s) 35, 160
PspGI CCWGG 2 cut(s) 35, 160
PspN4I GGNNCC 1 cut(s) 27
PvuII CAGCTG 1 cut(s) 322
RseI CAYNNNNRTG 1 cut(s) 305
SaqAI TTAA 3 cut(s) 315, 375, 440
SatI GCNGC 2 cut(s) 206, 370
Sau3AI GATC 1 cut(s) 119
SchI GAGTC 3 cut(s) 28, 205, 396
ScrFI CCNGG 2 cut(s) 37, 162
SduI GDGCHC 1 cut(s) 30
SetI ASST 6 cut(s) 68, 258, 324, 334, 374, 407
SfaNI GCATC 1 cut(s) 387
SfiI GGCCNNNNNGGCC 1 cut(s) 283
SmiMI CAYNNNNRTG 1 cut(s) 305
Sse9I AATT 1 cut(s) 71
SseBI AGGCCT 1 cut(s) 277
SsiI CCGC 4 cut(s) 100, 129, 203, 206
SspMI CTAG 1 cut(s) 191
StuI AGGCCT 1 cut(s) 277
StyD4I CCNGG 2 cut(s) 35, 160
TaqI TCGA 2 cut(s) 118, 385
TasI AATT 1 cut(s) 71
TauI GCSGC 1 cut(s) 208
TfiI GAWTC 2 cut(s) 115, 235
Tru1I TTAA 3 cut(s) 315, 375, 440
Tru9I TTAA 3 cut(s) 315, 375, 440
TseI GCWGC 1 cut(s) 369
TspDTI ATGAA 2 cut(s) 201, 408
TspGWI ACGGA 1 cut(s) 29
XapI RAATTY 1 cut(s) 71
XcmI CCANNNNNNNNNTGG 1 cut(s) 19
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.