Rw3G008020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0001309
Physical Location & Seq
Reverse (-)
7468473 .. 7473465
4993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G008020.1

Sequence Viewer

Length: 1320 bp
ATGGCTTCCACGGAGGGTCTGGTCCCCATAACCAGGGCTTTTCTCGCTTCTTATTACGACACCTACAATTTCCCTCCTCTCTCCGACGACATCCCCCGCCTCTCCTCCGAGATTCGCTCCCTCACCGCCGATTTGCTCTCCGACTCTCCCGCCACTCCAGGAGAAGAGAGATTAGTAATGGATGAGTTAGCGAGTCCGCCGCCGCATAAAGTGGATGAGAATATGTGGAAGAATCGGGAGCATATGGAAGAGATAGTCTTTTTACTTGACAGACCTCATTGGCCTCAATCGCTTCAAGAGCCTTCAAGTCCCGAGGATGCTCAGCTTGCTACTGTTTTTACTCGCCTGCATCATAAGTTCAATATGACTTTGAAGTCATTGGAATCGTTTCAAGCTAACAACTCTGAGCGTGTGTTCAATACAGTTATGACTTACATTCCTCAAGATTTTCGGGGGAGGCTTATTAGACTGCAGAGGGAGCGATCCGAGAGGAATAAGCAAGCGGAGATTGATGCTATGATTAGTTCTGGGGCAAGTATACGTGATCGTTATGCTCTCCTGTGGAAACAACAAATGGAGAGGAGGAGACTGTTGGCACAGCTTGGTTCTGCTACTGGTGTCTACAAAACCCTTGTCAAGTACTTGGTTGGGGTTCCACAGGTTTTACTAGATTTTGTTCGCCAAATAAATGATGATGATGGGCCCATGGAAGAGCAACGACAGCGTTATGGACCGCCTTTGTACAGCCTTACAACTATGGTGCTTCTTATACGTCTGGCTATTTTGCTGTCTCGGGGGCGGTTTGAAACTAGAAAATTAAACAAGCAAGAGGTAGCTGTCTTGGAACAAGCTGTTGACGTCTACACCACTGAGTTTGAAAGGTTTATTAGGTTTATCAGTGAGGTTTTTGCGAATTCGCCCTTCTTTATTTCAGCAGAGGTTGCCGGTGCATTAGATGGAGGGAATAATGATGATTACAAAGAGACAACTGTTCCTGCCGGTAAAACACATGAGGTTTCTCTGTCTGTGGACTCGATAAATTCGTATATTGCTTGGGATTTTTCGCTTTTGCAAGGGAAGATAAACTTGGATATCGGATTTAGTGTGGAGTATACAAATGCGTCTGGGGTTAAATCTCTCATTTTACCTTACAAACGTTACGAGTCTGACCAAGGGAATTTCTGCACGTGCGAGGCTGGAAACTACAAACTCATTTGGGACAATTCATATTCAAGTTTCTTTAAGAAGGTGCTGCGCTATAAGGTAGATTGTATACCTCCAGTTGTTGAAACGGTACCTCCTGTTGAAGTTGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

439

Amino Acids

50.19

Weight (kDa)

5.22

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 373
AatII GACGTC 1 cut(s) 861
Acc65I GGTACC 1 cut(s) 1294
AccB1I GGYRCC 1 cut(s) 1294
AccI GTMKAC 5 cut(s) 538, 621, 861, 1112, 1273
AciI CCGC 9 cut(s) 97, 126, 150, 197, 200, 203, 503, 734, 799
AclI AACGTT 1 cut(s) 1156
AclWI GGATC 1 cut(s) 477
AcsI RAATTY 3 cut(s) 913, 1039, 1177
AcvI CACGTG 1 cut(s) 1188
AcyI GRCGYC 1 cut(s) 858
AfaI GTAC 3 cut(s) 641, 743, 1296
AfiI CCNNNNNNNGG 1 cut(s) 33
AjnI CCWGG 2 cut(s) 32, 157
AjuI GAANNNNNNNTTGG 2 cut(s) 1070, 1102
AluBI AGCT 5 cut(s) 325, 395, 601, 836, 851
AluI AGCT 5 cut(s) 325, 395, 601, 836, 851
Alw26I GTCTC 3 cut(s) 580, 795, 977
AlwI GGATC 1 cut(s) 477
Ama87I CYCGRG 2 cut(s) 311, 792
AoxI GGCC 2 cut(s) 281, 701
ApaI GGGCCC 1 cut(s) 705
ApeKI GCWGC 1 cut(s) 1252
ApoI RAATTY 3 cut(s) 913, 1039, 1177
ArsI GACNNNNNNTTYG 2 cut(s) 618, 650
Asp700I GAANNNNTTC 1 cut(s) 387
Asp718I GGTACC 1 cut(s) 1294
AspLEI GCGC 1 cut(s) 1257
AspS9I GGNCC 4 cut(s) 22, 701, 702, 731
AsuHPI GGTGA 1 cut(s) 115
AvaI CYCGRG 2 cut(s) 311, 792
AvaII GGWCC 2 cut(s) 22, 731
BaeGI GKGCMC 1 cut(s) 705
BanI GGYRCC 1 cut(s) 1294
BanII GRGCYC 1 cut(s) 705
BbrPI CACGTG 1 cut(s) 1188
BbvI GCAGC 1 cut(s) 1239
BccI CCATC 2 cut(s) 692, 950
BciT130I CCWGG 2 cut(s) 34, 159
BcoDI GTCTC 3 cut(s) 580, 795, 977
BfaI CTAG 2 cut(s) 668, 810
BfmI CTRYAG 1 cut(s) 470
BisI GCNGC 3 cut(s) 200, 203, 1253
BlpI GCTNAGC 1 cut(s) 321
BlsI GCNGC 3 cut(s) 201, 204, 1254
BmcAI AGTACT 1 cut(s) 641
Bme1390I CCNGG 2 cut(s) 34, 159
Bme18I GGWCC 2 cut(s) 22, 731
BmeT110I CYCGRG 2 cut(s) 311, 792
BmgT120I GGNCC 4 cut(s) 22, 701, 702, 731
BmiI GGNNCC 4 cut(s) 24, 654, 703, 1296
BmrFI CCNGG 2 cut(s) 34, 159
BmsI GCATC 3 cut(s) 307, 358, 502
BplI GAGNNNNNCTC 2 cut(s) 101, 133
BpmI CTGGAG 2 cut(s) 141, 1263
Bpu1102I GCTNAGC 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 426
BsaAI YACGTR 2 cut(s) 542, 1188
BsaHI GRCGYC 1 cut(s) 858
BsaJI CCNNGG 5 cut(s) 9, 33, 312, 705, 1171
BsaXI ACNNNNNCTCC 2 cut(s) 1282, 1312
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse118I RCCGGY 2 cut(s) 944, 998
Bse1I ACTGG 2 cut(s) 619, 1280
BseBI CCWGG 2 cut(s) 34, 159
BseDI CCNNGG 5 cut(s) 9, 33, 312, 705, 1171
BseGI GGATG 4 cut(s) 90, 187, 220, 322
BseLI CCNNNNNNNGG 1 cut(s) 33
BseMII CTCAG 3 cut(s) 335, 396, 861
BseNI ACTGG 2 cut(s) 619, 1280
BseRI GAGGAG 4 cut(s) 66, 94, 595, 598
BseSI GKGCMC 1 cut(s) 705
BseXI GCAGC 1 cut(s) 1239
BsgI GTGCAG 1 cut(s) 1168
BshFI GGCC 2 cut(s) 283, 703
BshNI GGYRCC 1 cut(s) 1294
BsiHKCI CYCGRG 2 cut(s) 311, 792
BsiSI CCGG 2 cut(s) 945, 999
BslFI GGGAC 3 cut(s) 8, 294, 1232
BslI CCNNNNNNNGG 1 cut(s) 33
BsmAI GTCTC 3 cut(s) 580, 795, 977
BsmFI GGGAC 3 cut(s) 8, 294, 1232
BsnI GGCC 2 cut(s) 283, 703
BsoBI CYCGRG 2 cut(s) 311, 792
Bsp120I GGGCCC 1 cut(s) 701
Bsp1286I GDGCHC 1 cut(s) 705
Bsp1407I TGTACA 1 cut(s) 741
Bsp143I GATC 2 cut(s) 482, 544
Bsp1720I GCTNAGC 1 cut(s) 321
Bsp19I CCATGG 1 cut(s) 705
BspACI CCGC 9 cut(s) 97, 126, 150, 197, 200, 203, 503, 734, 799
BspANI GGCC 2 cut(s) 283, 703
BspCNI CTCAG 3 cut(s) 334, 397, 862
BspLI GGNNCC 4 cut(s) 24, 654, 703, 1296
BspMAI CTGCAG 1 cut(s) 474
BspPI GGATC 1 cut(s) 477
BspQI GCTCTTC 1 cut(s) 705
BspT107I GGYRCC 1 cut(s) 1294
BsrFI RCCGGY 2 cut(s) 944, 998
BsrGI TGTACA 1 cut(s) 741
BsrI ACTGG 2 cut(s) 619, 1280
BssAI RCCGGY 2 cut(s) 944, 998
BssECI CCNNGG 5 cut(s) 9, 33, 312, 705, 1171
BssMI GATC 2 cut(s) 482, 544
BssNAI GTATAC 3 cut(s) 539, 1113, 1274
BssNI GRCGYC 1 cut(s) 858
BssT1I CCWWGG 2 cut(s) 705, 1171
Bst1107I GTATAC 3 cut(s) 539, 1113, 1274
Bst2UI CCWGG 2 cut(s) 34, 159
Bst4CI ACNGT 5 cut(s) 334, 424, 591, 991, 1294
Bst6I CTCTTC 3 cut(s) 159, 243, 705
BstACI GRCGYC 1 cut(s) 858
BstAPI GCANNNNNTGC 1 cut(s) 941
BstAUI TGTACA 1 cut(s) 741
BstBAI YACGTR 2 cut(s) 542, 1188
BstC8I GCNNGC 3 cut(s) 327, 347, 501
BstDEI CTNAG 3 cut(s) 321, 405, 870
BstDSI CCRYGG 2 cut(s) 9, 705
BstF5I GGATG 4 cut(s) 90, 187, 220, 322
BstHHI GCGC 1 cut(s) 1257
BstKTI GATC 2 cut(s) 485, 547
BstMAI GTCTC 3 cut(s) 580, 795, 977
BstMBI GATC 2 cut(s) 482, 544
BstMWI GCNNNNNNNGC 7 cut(s) 44, 289, 298, 326, 478, 721, 941
BstNI CCWGG 2 cut(s) 34, 159
BstSCI CCNGG 2 cut(s) 32, 157
BstSFI CTRYAG 1 cut(s) 470
BstSLI GKGCMC 1 cut(s) 705
BstV1I GCAGC 1 cut(s) 1239
BstZ17I GTATAC 3 cut(s) 539, 1113, 1274
BsuRI GGCC 2 cut(s) 283, 703
BtgI CCRYGG 2 cut(s) 9, 705
BtsCI GGATG 4 cut(s) 90, 187, 220, 322
BtsIMutI CAGTG 2 cut(s) 867, 904
Cac8I GCNNGC 3 cut(s) 327, 347, 501
CfoI GCGC 1 cut(s) 1257
Cfr10I RCCGGY 2 cut(s) 944, 998
Cfr13I GGNCC 4 cut(s) 22, 701, 702, 731
CseI GACGC 1 cut(s) 1110
Csp6I GTAC 3 cut(s) 640, 742, 1295
CspCI CAANNNNNGTGG 2 cut(s) 856, 891
CviAII CATG 2 cut(s) 706, 1010
CviQI GTAC 3 cut(s) 640, 742, 1295
DdeI CTNAG 3 cut(s) 321, 405, 870
DpnI GATC 2 cut(s) 484, 546
DpnII GATC 2 cut(s) 482, 544
DrdI GACNNNNNNGTC 1 cut(s) 373
DseDI GACNNNNNNGTC 1 cut(s) 373
Eam1104I CTCTTC 3 cut(s) 159, 243, 705
EarI CTCTTC 3 cut(s) 159, 243, 705
EciI GGCGGA 1 cut(s) 186
Eco130I CCWWGG 2 cut(s) 705, 1171
Eco24I GRGCYC 1 cut(s) 705
Eco32I GATATC 1 cut(s) 1093
Eco47I GGWCC 2 cut(s) 22, 731
Eco72I CACGTG 1 cut(s) 1188
Eco88I CYCGRG 2 cut(s) 311, 792
EcoRI GAATTC 1 cut(s) 913
EcoRII CCWGG 2 cut(s) 32, 157
EcoRV GATATC 1 cut(s) 1093
EcoT14I CCWWGG 2 cut(s) 705, 1171
EcoT38I GRGCYC 1 cut(s) 705
ErhI CCWWGG 2 cut(s) 705, 1171
FaeI CATG 2 cut(s) 709, 1013
FalI AAGNNNNNCTT 2 cut(s) 1070, 1102
FaqI GGGAC 3 cut(s) 8, 294, 1232
FatI CATG 2 cut(s) 705, 1009
FauI CCCGC 2 cut(s) 104, 157
FauNDI CATATG 1 cut(s) 243
FblI GTMKAC 5 cut(s) 538, 621, 861, 1112, 1273
Fnu4HI GCNGC 3 cut(s) 200, 203, 1253
FokI GGATG 4 cut(s) 77, 194, 227, 329
FriOI GRGCYC 1 cut(s) 705
Fsp4HI GCNGC 3 cut(s) 200, 203, 1253
FspBI CTAG 2 cut(s) 668, 810
GlaI GCGC 1 cut(s) 1256
GluI GCNGC 3 cut(s) 200, 203, 1253
GsuI CTGGAG 2 cut(s) 141, 1263
HaeIII GGCC 2 cut(s) 283, 703
HapII CCGG 2 cut(s) 945, 999
HgaI GACGC 1 cut(s) 1110
HhaI GCGC 1 cut(s) 1257
Hin1I GRCGYC 1 cut(s) 858
Hin1II CATG 2 cut(s) 709, 1013
Hin6I GCGC 1 cut(s) 1255
HinP1I GCGC 1 cut(s) 1255
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HinfI GANTC 7 cut(s) 112, 143, 193, 232, 383, 1031, 1163
HpaII CCGG 2 cut(s) 945, 999
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 7 cut(s) 539, 622, 856, 862, 1030, 1113, 1274
Hpy188I TCNGA 7 cut(s) 85, 109, 142, 406, 487, 1097, 1168
Hpy188III TCNNGA 4 cut(s) 236, 296, 311, 443
Hpy8I GTNNAC 7 cut(s) 539, 622, 856, 862, 1030, 1113, 1274
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 3 cut(s) 312, 931, 1240
HpyCH4III ACNGT 5 cut(s) 334, 424, 591, 991, 1294
HpyCH4IV ACGT 5 cut(s) 541, 772, 858, 1156, 1187
HpyCH4V TGCA 5 cut(s) 349, 472, 950, 1072, 1185
HpyF10VI GCNNNNNNNGC 7 cut(s) 44, 289, 298, 326, 478, 721, 941
HpyF3I CTNAG 3 cut(s) 321, 405, 870
HpySE526I ACGT 5 cut(s) 541, 772, 858, 1156, 1187
Hsp92I GRCGYC 1 cut(s) 858
Hsp92II CATG 2 cut(s) 709, 1013
HspAI GCGC 1 cut(s) 1255
KpnI GGTACC 1 cut(s) 1298
Kzo9I GATC 2 cut(s) 482, 544
LguI GCTCTTC 1 cut(s) 705
LmnI GCTCC 3 cut(s) 122, 238, 478
Lsp1109I GCAGC 1 cut(s) 1239
LweI GCATC 3 cut(s) 307, 358, 502
MaeI CTAG 2 cut(s) 668, 810
MaeII ACGT 5 cut(s) 541, 772, 858, 1156, 1187
MaeIII GTNAC 1 cut(s) 1157
MalI GATC 2 cut(s) 484, 546
MboI GATC 2 cut(s) 482, 544
MboII GAAGA 5 cut(s) 176, 241, 260, 722, 1090
MhlI GDGCHC 1 cut(s) 705
MluCI AATT 6 cut(s) 67, 815, 913, 1039, 1177, 1222
MlyI GAGTC 4 cut(s) 137, 202, 1025, 1172
MmeI TCCRAC 2 cut(s) 108, 165
MroXI GAANNNNTTC 1 cut(s) 387
MseI TTAA 3 cut(s) 818, 1131, 1242
MspI CCGG 2 cut(s) 945, 999
MspR9I CCNGG 2 cut(s) 34, 159
MvaI CCWGG 2 cut(s) 34, 159
MwoI GCNNNNNNNGC 7 cut(s) 44, 289, 298, 326, 478, 721, 941
NcoI CCATGG 1 cut(s) 705
NdeI CATATG 1 cut(s) 243
NdeII GATC 2 cut(s) 482, 544
NlaIII CATG 2 cut(s) 709, 1013
NlaIV GGNNCC 4 cut(s) 24, 654, 703, 1296
PciSI GCTCTTC 1 cut(s) 705
PcsI WCGNNNNNNNCGW 1 cut(s) 1040
PdmI GAANNNNTTC 1 cut(s) 387
PfeI GAWTC 3 cut(s) 112, 232, 383
PfoI TCCNGGA 1 cut(s) 157
PkrI GCNGC 3 cut(s) 201, 204, 1254
PleI GAGTC 4 cut(s) 137, 201, 1025, 1171
PmaCI CACGTG 1 cut(s) 1188
PmlI CACGTG 1 cut(s) 1188
PpsI GAGTC 4 cut(s) 137, 201, 1025, 1171
Ppu21I YACGTR 2 cut(s) 542, 1188
Psp1406I AACGTT 1 cut(s) 1156
Psp6I CCWGG 2 cut(s) 32, 157
PspCI CACGTG 1 cut(s) 1188
PspGI CCWGG 2 cut(s) 32, 157
PspN4I GGNNCC 4 cut(s) 24, 654, 703, 1296
PspOMI GGGCCC 1 cut(s) 701
PspPI GGNCC 4 cut(s) 22, 701, 702, 731
PstI CTGCAG 1 cut(s) 474
RsaI GTAC 3 cut(s) 641, 743, 1296
RsaNI GTAC 3 cut(s) 640, 742, 1295
SapI GCTCTTC 1 cut(s) 705
SaqAI TTAA 3 cut(s) 818, 1131, 1242
SatI GCNGC 3 cut(s) 200, 203, 1253
Sau3AI GATC 2 cut(s) 482, 544
Sau96I GGNCC 4 cut(s) 22, 701, 702, 731
ScaI AGTACT 1 cut(s) 641
SchI GAGTC 4 cut(s) 137, 202, 1025, 1172
ScrFI CCNGG 2 cut(s) 34, 159
SduI GDGCHC 1 cut(s) 705
SfaNI GCATC 3 cut(s) 307, 358, 502
SfcI CTRYAG 1 cut(s) 470
SinI GGWCC 2 cut(s) 22, 731
SmlI CTYRAG 1 cut(s) 441
SmoI CTYRAG 1 cut(s) 441
Sse9I AATT 6 cut(s) 67, 815, 913, 1039, 1177, 1222
SsiI CCGC 9 cut(s) 97, 126, 150, 197, 200, 203, 503, 734, 799
SspMI CTAG 2 cut(s) 668, 810
StyD4I CCNGG 2 cut(s) 32, 157
StyI CCWWGG 2 cut(s) 705, 1171
TaaI ACNGT 5 cut(s) 334, 424, 591, 991, 1294
TaiI ACGT 5 cut(s) 544, 775, 861, 1159, 1190
TaqI TCGA 1 cut(s) 1034
TasI AATT 6 cut(s) 67, 815, 913, 1039, 1177, 1222
TatI WGTACW 2 cut(s) 639, 741
TauI GCSGC 2 cut(s) 202, 205
TfiI GAWTC 3 cut(s) 112, 232, 383
Tru1I TTAA 3 cut(s) 818, 1131, 1242
Tru9I TTAA 3 cut(s) 818, 1131, 1242
TscAI CASTG 2 cut(s) 874, 904
TseI GCWGC 1 cut(s) 1252
TspDTI ATGAA 1 cut(s) 1215
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 2 cut(s) 874, 904
VpaK11BI GGWCC 2 cut(s) 22, 731
XapI RAATTY 3 cut(s) 913, 1039, 1177
XcmI CCANNNNNNNNNTGG 1 cut(s) 16
XmiI GTMKAC 5 cut(s) 538, 621, 861, 1112, 1273
XmnI GAANNNNTTC 1 cut(s) 387
XspI CTAG 2 cut(s) 668, 810
ZraI GACGTC 1 cut(s) 859
ZrmI AGTACT 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.