AT5G01010

EXPRESSED DURING 14 growth stages

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001309
Physical Location & Seq
Reverse (-)
995 .. 5156
4162 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G01010.2

Sequence Viewer

Length: 1317 bp
ATGGCGTCAACGGAAGGCCTTATGCCTATCACCCGGGCTTTTCTCGCTTCTTACTACGACAAATATCCTTTCTCTCCTCTTTCCGACGACGTTTCTCGGCTTTCCTCTGACATGGCATCTCTGATTAAGCTATTGACCGTCCAATCTCCCCCTTCACAAGGGGAAACTTCCCTGATTGACGAAGCAAACCGTCAGCCCCCTCACAAAATTGATGAGAATATGTGGAAAAATAGGGAGCAGATGGAGGAGATACTGTTTCTTCTGTCACCATCTCGCTGGCCTGTTCAGCTTCGGGAACCTTCCACATCCGAAGATGCTGAATTCGCTTCTATTTTGCGGACTCTCAAGGACAGTTTTGATAACGCATTCACTGCTATGATTTCCTTCCAGACGAAGAATTCCGAGCGCATATTCAGCACAGTAATGACCTACATGCCTCAAGATTTCCGGGGAACGCTCATCAGACAGCAGAAGGAGAGGTCGGAGAGGAACAAACAGGCGGAGGTTGATGCGTTGGTCAGCTCTGGAGGGAGCATACGCGACACATATGCTCTTCTTTGGAAGCAGCAAATGGAGAGGAGGAGGCAGTTGGCTCAGCTAGGCTCTGCCACTGGTGTCTACAAAACCCTGGTCAAGTACTTGGTAGGGGTTCCACAGGTGTTGTTGGATTTTATTCGACAAATAAACGATGATGATGGGCCAATGGAAGAACAGCGAGAACGTTATGGACCCCCTCTATACAGTCTCACAAAAATGGTGATCGCAATTCGGGTTTTCCTAACACTTCTGTGGGAAAGATATGATACCTTCAAACTGAGTAAAGACCAAATGAATCTCCTGTCTGAAGCTGCTATAGTCTACACTTCTGAGTTTGAAAGATTCGTCACATTTATCAGCGATGTATTCGCCAATTCCCCCTTCTTTATTTCAGCTGATACAGCTGGCATATTGTGGTCAAGGGACAATGAGGAGTACAAGGAGATAATCGTTCAAGCTGGGAGAACCTATGAGATCTCATTGATGGTGGAATCTGAAAATTCGTATATTGCTTGGGATTTCTCTTTGATGCAAGGCAAGATAAGCATGGATATCGGATTTAGCGTGGAGTACATAAATGCTTCAGGGGAAAAGACTCTGATATTACCTTACCGTCGGTATGAAGCTGACCAGGGCAACTTCTCTACGCTAATGGCTGGAAACTACAAACTTGTTTGGGATAATTCATATTCAACTTTCTTCAAAAAGACTCTTCGATATAAGGTGGATTGCATAGCACCAGTAGTGGAGCCGGATCCAGAGCCGGAGCCGTTAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

438

Amino Acids

50.43

Weight (kDa)

4.91

Isoelectric Point (pI)

49.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 618, 858
AccII CGCG 1 cut(s) 540
AciI CCGC 2 cut(s) 337, 500
AclI AACGTT 1 cut(s) 721
AclWI GGATC 2 cut(s) 1286, 1299
AcsI RAATTY 3 cut(s) 320, 397, 1036
AcuI CTGAAG 2 cut(s) 864, 1104
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 638, 974, 1109
AfiI CCNNNNNNNGG 1 cut(s) 158
AgsI TTSAA 5 cut(s) 811, 875, 992, 1230, 1240
AjnI CCWGG 2 cut(s) 627, 1167
AjuI GAANNNNNNNTTGG 2 cut(s) 902, 934
AleI CACNNNNGTG 1 cut(s) 787
AluBI AGCT 9 cut(s) 130, 289, 522, 598, 848, 932, 941, 995, 1163
AluI AGCT 9 cut(s) 130, 289, 522, 598, 848, 932, 941, 995, 1163
Alw26I GTCTC 1 cut(s) 749
AlwI GGATC 2 cut(s) 1286, 1299
Ama87I CYCGRG 1 cut(s) 33
AoxI GGCC 3 cut(s) 16, 278, 698
ApeKI GCWGC 2 cut(s) 565, 848
ApoI RAATTY 3 cut(s) 320, 397, 1036
ArsI GACNNNNNNTTYG 2 cut(s) 615, 647
AspLEI GCGC 1 cut(s) 408
AspS9I GGNCC 2 cut(s) 698, 728
AsuC2I CCSGG 3 cut(s) 34, 35, 449
AsuHPI GGTGA 3 cut(s) 22, 258, 769
AvaI CYCGRG 1 cut(s) 33
AvaII GGWCC 1 cut(s) 728
BamHI GGATCC 1 cut(s) 1291
BbvI GCAGC 2 cut(s) 577, 835
BccI CCATC 4 cut(s) 235, 277, 689, 1015
BceAI ACGGC 1 cut(s) 1291
BcgI CGANNNNNNTGC 4 cut(s) 530, 564, 1072, 1106
BciT130I CCWGG 2 cut(s) 629, 1169
BcnI CCSGG 3 cut(s) 34, 35, 449
BcoDI GTCTC 1 cut(s) 749
BfaI CTAG 1 cut(s) 599
BfmI CTRYAG 1 cut(s) 852
BglII AGATCT 1 cut(s) 1011
BisI GCNGC 2 cut(s) 566, 849
BlpI GCTNAGC 1 cut(s) 594
BlsI GCNGC 2 cut(s) 567, 850
BmcAI AGTACT 1 cut(s) 638
Bme1390I CCNGG 5 cut(s) 34, 35, 449, 629, 1169
Bme18I GGWCC 1 cut(s) 728
BmeT110I CYCGRG 1 cut(s) 33
BmgT120I GGNCC 2 cut(s) 698, 728
BmiI GGNNCC 6 cut(s) 297, 651, 730, 1287, 1293, 1305
BmrFI CCNGG 5 cut(s) 34, 35, 449, 629, 1169
BmsI GCATC 4 cut(s) 125, 304, 499, 1056
BpmI CTGGAG 1 cut(s) 546
Bpu1102I GCTNAGC 1 cut(s) 594
BpuEI CTTGAG 2 cut(s) 329, 423
BpuMI CCSGG 3 cut(s) 34, 35, 449
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 4 cut(s) 33, 448, 627, 1168
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 1 cut(s) 158
Bse1I ACTGG 2 cut(s) 616, 1277
BseBI CCWGG 2 cut(s) 629, 1169
BseDI CCNNGG 4 cut(s) 33, 448, 627, 1168
BseGI GGATG 1 cut(s) 305
BseLI CCNNNNNNNGG 1 cut(s) 158
BseMII CTCAG 3 cut(s) 608, 806, 858
BseNI ACTGG 2 cut(s) 616, 1277
BseRI GAGGAG 5 cut(s) 66, 260, 592, 595, 983
BseXI GCAGC 2 cut(s) 577, 835
BseYI CCCAGC 1 cut(s) 995
Bsh1236I CGCG 1 cut(s) 540
BshFI GGCC 3 cut(s) 18, 280, 700
BsiHKCI CYCGRG 1 cut(s) 33
BsiSI CCGG 4 cut(s) 34, 448, 1289, 1301
BslFI GGGAC 1 cut(s) 974
BslI CCNNNNNNNGG 1 cut(s) 158
BsmAI GTCTC 1 cut(s) 749
BsmFI GGGAC 1 cut(s) 974
BsmI GAATGC 1 cut(s) 365
BsnI GGCC 3 cut(s) 18, 280, 700
BsoBI CYCGRG 1 cut(s) 33
Bsp143I GATC 3 cut(s) 759, 1011, 1291
Bsp1720I GCTNAGC 1 cut(s) 594
BspACI CCGC 2 cut(s) 337, 500
BspANI GGCC 3 cut(s) 18, 280, 700
BspCNI CTCAG 3 cut(s) 607, 807, 859
BspFNI CGCG 1 cut(s) 540
BspLI GGNNCC 6 cut(s) 297, 651, 730, 1287, 1293, 1305
BspPI GGATC 2 cut(s) 1286, 1299
BspQI GCTCTTC 1 cut(s) 558
BsrI ACTGG 2 cut(s) 616, 1277
BssECI CCNNGG 4 cut(s) 33, 448, 627, 1168
BssMI GATC 3 cut(s) 759, 1011, 1291
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 2 cut(s) 629, 1169
Bst4CI ACNGT 7 cut(s) 139, 191, 255, 353, 421, 743, 1151
Bst6I CTCTTC 2 cut(s) 558, 1254
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 371
BstC8I GCNNGC 2 cut(s) 278, 943
BstDEI CTNAG 3 cut(s) 594, 815, 867
BstENI CCTNNNNNAGG 1 cut(s) 156
BstF5I GGATG 1 cut(s) 305
BstFNI CGCG 1 cut(s) 540
BstHHI GCGC 1 cut(s) 408
BstKTI GATC 3 cut(s) 762, 1014, 1294
BstMAI GTCTC 1 cut(s) 749
BstMBI GATC 3 cut(s) 759, 1011, 1291
BstMWI GCNNNNNNNGC 7 cut(s) 44, 286, 323, 371, 414, 938, 1080
BstNI CCWGG 2 cut(s) 629, 1169
BstNSI RCATGY 1 cut(s) 436
BstSCI CCNGG 5 cut(s) 32, 33, 447, 627, 1167
BstSFI CTRYAG 1 cut(s) 852
BstUI CGCG 1 cut(s) 540
BstV1I GCAGC 2 cut(s) 577, 835
BstX2I RGATCY 2 cut(s) 1011, 1291
BstXI CCANNNNNNTGG 1 cut(s) 276
BstYI RGATCY 2 cut(s) 1011, 1291
BsuRI GGCC 3 cut(s) 18, 280, 700
BtgZI GCGATG 1 cut(s) 912
BtsCI GGATG 1 cut(s) 305
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 2 cut(s) 369, 609
Cac8I GCNNGC 2 cut(s) 278, 943
CfoI GCGC 1 cut(s) 408
Cfr13I GGNCC 2 cut(s) 698, 728
Cfr9I CCCGGG 1 cut(s) 33
Csp6I GTAC 3 cut(s) 637, 973, 1108
CspCI CAANNNNNGTGG 2 cut(s) 642, 677
CviAII CATG 3 cut(s) 112, 433, 1084
CviQI GTAC 3 cut(s) 637, 973, 1108
DdeI CTNAG 3 cut(s) 594, 815, 867
DpnI GATC 3 cut(s) 761, 1013, 1293
DpnII GATC 3 cut(s) 759, 1011, 1291
Eam1104I CTCTTC 2 cut(s) 558, 1254
EarI CTCTTC 2 cut(s) 558, 1254
EciI GGCGGA 1 cut(s) 515
Eco147I AGGCCT 1 cut(s) 18
Eco32I GATATC 1 cut(s) 1090
Eco47I GGWCC 1 cut(s) 728
Eco57I CTGAAG 2 cut(s) 864, 1104
Eco88I CYCGRG 1 cut(s) 33
EcoNI CCTNNNNNAGG 1 cut(s) 156
EcoRI GAATTC 2 cut(s) 320, 397
EcoRII CCWGG 2 cut(s) 627, 1167
EcoRV GATATC 1 cut(s) 1090
FaeI CATG 3 cut(s) 115, 436, 1087
FaqI GGGAC 1 cut(s) 974
FatI CATG 3 cut(s) 111, 432, 1083
FauNDI CATATG 1 cut(s) 547
FblI GTMKAC 2 cut(s) 618, 858
Fnu4HI GCNGC 2 cut(s) 566, 849
FokI GGATG 1 cut(s) 292
Fsp4HI GCNGC 2 cut(s) 566, 849
FspBI CTAG 1 cut(s) 599
GlaI GCGC 1 cut(s) 407
GluI GCNGC 2 cut(s) 566, 849
GsaI CCCAGC 1 cut(s) 999
GsuI CTGGAG 1 cut(s) 546
HaeIII GGCC 3 cut(s) 18, 280, 700
HapII CCGG 4 cut(s) 34, 448, 1289, 1301
HhaI GCGC 1 cut(s) 408
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 115, 436, 1087
Hin6I GCGC 1 cut(s) 406
HinP1I GCGC 1 cut(s) 406
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HinfI GANTC 6 cut(s) 340, 832, 879, 1028, 1132, 1246
HpaII CCGG 4 cut(s) 34, 448, 1289, 1301
HphI GGTGA 3 cut(s) 22, 258, 769
Hpy166II GTNNAC 3 cut(s) 9, 619, 859
Hpy188III TCNNGA 5 cut(s) 293, 388, 440, 525, 1295
Hpy8I GTNNAC 3 cut(s) 9, 619, 859
Hpy99I CGWCG 3 cut(s) 89, 92, 1155
HpyAV CCTTC 7 cut(s) 8, 162, 309, 394, 466, 817, 928
HpyCH4III ACNGT 7 cut(s) 139, 191, 255, 353, 421, 743, 1151
HpyCH4IV ACGT 2 cut(s) 90, 721
HpyCH4V TGCA 2 cut(s) 1069, 1269
HpyF10VI GCNNNNNNNGC 7 cut(s) 44, 286, 323, 371, 414, 938, 1080
HpyF3I CTNAG 3 cut(s) 594, 815, 867
HpySE526I ACGT 2 cut(s) 90, 721
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 115, 436, 1087
HspAI GCGC 1 cut(s) 406
Kzo9I GATC 3 cut(s) 759, 1011, 1291
LguI GCTCTTC 1 cut(s) 558
LmnI GCTCC 4 cut(s) 235, 531, 1285, 1303
Lsp1109I GCAGC 2 cut(s) 577, 835
LweI GCATC 4 cut(s) 125, 304, 499, 1056
MaeI CTAG 1 cut(s) 599
MaeII ACGT 2 cut(s) 90, 721
MaeIII GTNAC 2 cut(s) 264, 883
MalI GATC 3 cut(s) 761, 1013, 1293
MboI GATC 3 cut(s) 759, 1011, 1291
MboII GAAGA 7 cut(s) 251, 323, 406, 545, 719, 1228, 1241
MflI RGATCY 2 cut(s) 1011, 1291
MluCI AATT 7 cut(s) 207, 320, 397, 765, 910, 1036, 1219
MlyI GAGTC 3 cut(s) 334, 1126, 1240
MmeI TCCRAC 3 cut(s) 108, 462, 645
MseI TTAA 2 cut(s) 126, 1310
MslI CAYNNNNRTG 4 cut(s) 374, 422, 752, 787
MspA1I CMGCKG 2 cut(s) 932, 941
MspI CCGG 4 cut(s) 34, 448, 1289, 1301
MspR9I CCNGG 5 cut(s) 34, 35, 449, 629, 1169
Mva1269I GAATGC 1 cut(s) 365
MvaI CCWGG 2 cut(s) 629, 1169
MvnI CGCG 1 cut(s) 540
MwoI GCNNNNNNNGC 7 cut(s) 44, 286, 323, 371, 414, 938, 1080
NciI CCSGG 3 cut(s) 34, 35, 449
NdeI CATATG 1 cut(s) 547
NdeII GATC 3 cut(s) 759, 1011, 1291
NlaIII CATG 3 cut(s) 115, 436, 1087
NlaIV GGNNCC 6 cut(s) 297, 651, 730, 1287, 1293, 1305
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 2 cut(s) 264, 883
NspI RCATGY 1 cut(s) 436
OliI CACNNNNGTG 1 cut(s) 787
PceI AGGCCT 1 cut(s) 18
PciSI GCTCTTC 1 cut(s) 558
PcsI WCGNNNNNNNCGW 1 cut(s) 1098
PctI GAATGC 1 cut(s) 365
PfeI GAWTC 3 cut(s) 832, 879, 1028
PkrI GCNGC 2 cut(s) 567, 850
PleI GAGTC 3 cut(s) 334, 1126, 1240
PpsI GAGTC 3 cut(s) 334, 1126, 1240
Psp1406I AACGTT 1 cut(s) 721
Psp6I CCWGG 2 cut(s) 627, 1167
PspFI CCCAGC 1 cut(s) 995
PspGI CCWGG 2 cut(s) 627, 1167
PspN4I GGNNCC 6 cut(s) 297, 651, 730, 1287, 1293, 1305
PspPI GGNCC 2 cut(s) 698, 728
PsuI RGATCY 2 cut(s) 1011, 1291
PvuII CAGCTG 2 cut(s) 932, 941
RsaI GTAC 3 cut(s) 638, 974, 1109
RsaNI GTAC 3 cut(s) 637, 973, 1108
RseI CAYNNNNRTG 4 cut(s) 374, 422, 752, 787
SapI GCTCTTC 1 cut(s) 558
SaqAI TTAA 2 cut(s) 126, 1310
SatI GCNGC 2 cut(s) 566, 849
Sau3AI GATC 3 cut(s) 759, 1011, 1291
Sau96I GGNCC 2 cut(s) 698, 728
ScaI AGTACT 1 cut(s) 638
SchI GAGTC 3 cut(s) 334, 1126, 1240
ScrFI CCNGG 5 cut(s) 34, 35, 449, 629, 1169
SfaNI GCATC 4 cut(s) 125, 304, 499, 1056
SfcI CTRYAG 1 cut(s) 852
SinI GGWCC 1 cut(s) 728
SmaI CCCGGG 1 cut(s) 35
SmiMI CAYNNNNRTG 4 cut(s) 374, 422, 752, 787
SmlI CTYRAG 2 cut(s) 344, 438
SmoI CTYRAG 2 cut(s) 344, 438
Sse9I AATT 7 cut(s) 207, 320, 397, 765, 910, 1036, 1219
SseBI AGGCCT 1 cut(s) 18
SsiI CCGC 2 cut(s) 337, 500
SspMI CTAG 1 cut(s) 599
StuI AGGCCT 1 cut(s) 18
StyD4I CCNGG 5 cut(s) 32, 33, 447, 627, 1167
TaaI ACNGT 7 cut(s) 139, 191, 255, 353, 421, 743, 1151
TaiI ACGT 2 cut(s) 93, 724
TaqI TCGA 2 cut(s) 676, 1252
TasI AATT 7 cut(s) 207, 320, 397, 765, 910, 1036, 1219
TatI WGTACW 3 cut(s) 636, 972, 1107
TfiI GAWTC 3 cut(s) 832, 879, 1028
Tru1I TTAA 2 cut(s) 126, 1310
Tru9I TTAA 2 cut(s) 126, 1310
TscAI CASTG 2 cut(s) 376, 616
TseFI GTSAC 2 cut(s) 264, 883
TseI GCWGC 2 cut(s) 565, 848
Tsp45I GTSAC 2 cut(s) 264, 883
TspDTI ATGAA 3 cut(s) 845, 1173, 1212
TspGWI ACGGA 1 cut(s) 26
TspMI CCCGGG 1 cut(s) 33
TspRI CASTG 2 cut(s) 376, 616
VpaK11BI GGWCC 1 cut(s) 728
XagI CCTNNNNNAGG 1 cut(s) 156
XapI RAATTY 3 cut(s) 320, 397, 1036
XceI RCATGY 1 cut(s) 436
XmaI CCCGGG 1 cut(s) 33
XmiI GTMKAC 2 cut(s) 618, 858
XspI CTAG 1 cut(s) 599
ZrmI AGTACT 1 cut(s) 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.