Rroxscaffold_6G00420280

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001309
Physical Location & Seq
Forward (+)
41575936 .. 41581065
5130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00420280.1

Sequence Viewer

Length: 1308 bp
ATGGCTTCCACGGAGGGTCTGGTCCCCATAACCAGGGCTTTTCTCGCTTCTTATTACGACACCTACAATTTCCCTCCTCTCTCCGACGACATCCCCCGCCTCTCCTCCGAGATTCGCTCCCTCACCGCCGATTTGCTCTCCAACTCTCCCGCCACTCCAGGAGAAGAGAGATTAGTAATGGATGAGTTAGCGAGTCCGCCGCCGCATAAAGTGGATGAGAATATGTGGAAGAATCGGGAGCATATGGAAGAGATAGTCTTTTTACTCGACAGACCTCATTGGCCTCAATCGCTTCAAGAGCCTTCGACGTGTGTTCAATACAGGTTTTGTTTGATCTCTGTTGTTAATGTTGATGTTTTGGAATTGGGATGGTTTAGTAATGGGAGTGTGAATGTGTTTTGTGTTTGCAGTTATGACTTACATTCCTCAAGATTTTCGGGGAGGCTTATTAGACTGCAGAGGGAGCGATCCGAGAGGAATAAGCAAGCAGAGATTGATGCTATGATTAGTTCTGGGGCAAGTATACGTGATCGTTATGCTGTCTTGTGGAAACAACAAATGGAGAGGAGAAGACTGTTGGCGCAGCTTGGTTCTGCTACTGGTGTCTACAAAACCCTTGTCAAGTACTTGGTTGGGGTTCCACAGGTTTTACTAGATTTTGTTCGCCAAATAAATGATGATGATGGGCCAATGGAAGAGCAACGAGAGCGTTATGGACCACCTTTGTACAGCCTTACAACTATGGTGCTTCTTATACGTTTGGCTATTTTGCTTTCTTGGGGACGGTTTGAAACTAGAAAATTAAACAAGCAAGAGGTAGCTGTCTTGGAACAAGCTATTGACGTCTACACCACTGAGTTTGAAAGGTTTATTAGGTTTATCAGTGAGGTTTTTGCGAATTCGCCCTTCTTTATTTCAGCAGAGGTTGCCGGTGCGTTAGATGGAGGGAATAATGATGATTACAAAGAGACAACTGTTCCTGCCGGTAAAACACATGAGGTTTCTCTGTCTGTGGACTCGATAAATTCGTATATTGCTTGGGATTTTTCGCTTGTGCAAGGGAAGATAAACTTGGATATTGGATTTAGTGTGGAGTATACAAATGCGTCTGGGGAGAAATCTCTCATTTTACCTTACAAACGTTACGAGTCTGACCAAGGGAATTTCTGCACGTGCGAGGCTGGAAACTACAAACTCGTTTGGGACAATTCATATTCAAGTTTCTTTAAGAAGGTGCTGCGCTATAAGGTAGATTGTATACCTCCAGTTGTTGAAACGGTGCCTCCTGCGACTGAAGTTGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

435

Amino Acids

49.53

Weight (kDa)

5.0

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 846
AccB1I GGYRCC 1 cut(s) 1279
AccI GTMKAC 5 cut(s) 523, 606, 846, 1097, 1258
AciI CCGC 6 cut(s) 97, 126, 150, 197, 200, 203
AclI AACGTT 1 cut(s) 1141
AclWI GGATC 1 cut(s) 462
AcsI RAATTY 3 cut(s) 898, 1024, 1162
AcvI CACGTG 1 cut(s) 1173
AcyI GRCGYC 1 cut(s) 843
AfaI GTAC 2 cut(s) 626, 728
AfiI CCNNNNNNNGG 1 cut(s) 33
AflIII ACRYGT 1 cut(s) 308
AgsI TTSAA 7 cut(s) 296, 317, 791, 863, 1218, 1274, 1301
AjiI CACGTC 1 cut(s) 309
AjnI CCWGG 2 cut(s) 32, 157
AjuI GAANNNNNNNTTGG 2 cut(s) 1055, 1087
AluBI AGCT 3 cut(s) 586, 821, 836
AluI AGCT 3 cut(s) 586, 821, 836
Alw26I GTCTC 1 cut(s) 962
AlwI GGATC 1 cut(s) 462
AoxI GGCC 2 cut(s) 281, 686
ApeKI GCWGC 2 cut(s) 583, 1237
ApoI RAATTY 3 cut(s) 898, 1024, 1162
ArsI GACNNNNNNTTYG 2 cut(s) 603, 635
AspLEI GCGC 2 cut(s) 583, 1242
AspS9I GGNCC 3 cut(s) 22, 686, 716
AsuHPI GGTGA 1 cut(s) 115
AvaII GGWCC 2 cut(s) 22, 716
BanI GGYRCC 1 cut(s) 1279
BbrPI CACGTG 1 cut(s) 1173
BbsI GAAGAC 1 cut(s) 577
BbvI GCAGC 2 cut(s) 595, 1224
BccI CCATC 3 cut(s) 363, 677, 935
BciT130I CCWGG 2 cut(s) 34, 159
BcoDI GTCTC 1 cut(s) 962
BfaI CTAG 2 cut(s) 653, 795
BfmI CTRYAG 1 cut(s) 455
BisI GCNGC 4 cut(s) 200, 203, 584, 1238
BlsI GCNGC 4 cut(s) 201, 204, 585, 1239
BmcAI AGTACT 1 cut(s) 626
Bme1390I CCNGG 2 cut(s) 34, 159
Bme18I GGWCC 2 cut(s) 22, 716
BmgBI CACGTC 1 cut(s) 309
BmgT120I GGNCC 3 cut(s) 22, 686, 716
BmiI GGNNCC 3 cut(s) 24, 639, 1281
BmrFI CCNGG 2 cut(s) 34, 159
BmsI GCATC 1 cut(s) 487
BpiI GAAGAC 1 cut(s) 577
BplI GAGNNNNNCTC 2 cut(s) 101, 133
BpmI CTGGAG 2 cut(s) 141, 1248
BpuEI CTTGAG 1 cut(s) 412
BsaAI YACGTR 2 cut(s) 527, 1173
BsaHI GRCGYC 1 cut(s) 843
BsaJI CCNNGG 3 cut(s) 9, 33, 1156
BsaXI ACNNNNNCTCC 2 cut(s) 1267, 1297
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse118I RCCGGY 2 cut(s) 929, 983
Bse1I ACTGG 2 cut(s) 604, 1265
BseBI CCWGG 2 cut(s) 34, 159
BseDI CCNNGG 3 cut(s) 9, 33, 1156
BseGI GGATG 4 cut(s) 90, 187, 220, 374
BseLI CCNNNNNNNGG 1 cut(s) 33
BseMII CTCAG 1 cut(s) 846
BseNI ACTGG 2 cut(s) 604, 1265
BseRI GAGGAG 3 cut(s) 66, 94, 580
BseXI GCAGC 2 cut(s) 595, 1224
BsgI GTGCAG 1 cut(s) 1153
BshFI GGCC 2 cut(s) 283, 688
BshNI GGYRCC 1 cut(s) 1279
BsiSI CCGG 2 cut(s) 930, 984
BslFI GGGAC 3 cut(s) 8, 795, 1217
BslI CCNNNNNNNGG 1 cut(s) 33
BsmAI GTCTC 1 cut(s) 962
BsmFI GGGAC 3 cut(s) 8, 795, 1217
BsnI GGCC 2 cut(s) 283, 688
Bsp1407I TGTACA 1 cut(s) 726
Bsp143I GATC 3 cut(s) 333, 467, 529
BspACI CCGC 6 cut(s) 97, 126, 150, 197, 200, 203
BspANI GGCC 2 cut(s) 283, 688
BspCNI CTCAG 1 cut(s) 847
BspLI GGNNCC 3 cut(s) 24, 639, 1281
BspMAI CTGCAG 1 cut(s) 459
BspPI GGATC 1 cut(s) 462
BspQI GCTCTTC 1 cut(s) 690
BspT107I GGYRCC 1 cut(s) 1279
BsrFI RCCGGY 2 cut(s) 929, 983
BsrGI TGTACA 1 cut(s) 726
BsrI ACTGG 2 cut(s) 604, 1265
BssAI RCCGGY 2 cut(s) 929, 983
BssECI CCNNGG 3 cut(s) 9, 33, 1156
BssMI GATC 3 cut(s) 333, 467, 529
BssNAI GTATAC 3 cut(s) 524, 1098, 1259
BssNI GRCGYC 1 cut(s) 843
BssT1I CCWWGG 1 cut(s) 1156
Bst1107I GTATAC 3 cut(s) 524, 1098, 1259
Bst2UI CCWGG 2 cut(s) 34, 159
Bst4CI ACNGT 4 cut(s) 576, 786, 976, 1279
Bst6I CTCTTC 3 cut(s) 159, 243, 690
BstACI GRCGYC 1 cut(s) 843
BstAPI GCANNNNNTGC 1 cut(s) 926
BstAUI TGTACA 1 cut(s) 726
BstBAI YACGTR 2 cut(s) 527, 1173
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 1 cut(s) 855
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 4 cut(s) 90, 187, 220, 374
BstHHI GCGC 2 cut(s) 583, 1242
BstKTI GATC 3 cut(s) 336, 470, 532
BstMAI GTCTC 1 cut(s) 962
BstMBI GATC 3 cut(s) 333, 467, 529
BstMWI GCNNNNNNNGC 6 cut(s) 44, 289, 298, 463, 706, 926
BstNI CCWGG 2 cut(s) 34, 159
BstSCI CCNGG 2 cut(s) 32, 157
BstSFI CTRYAG 1 cut(s) 455
BstV1I GCAGC 2 cut(s) 595, 1224
BstV2I GAAGAC 1 cut(s) 577
BstZ17I GTATAC 3 cut(s) 524, 1098, 1259
BsuRI GGCC 2 cut(s) 283, 688
BtgI CCRYGG 1 cut(s) 9
BtrI CACGTC 1 cut(s) 309
BtsCI GGATG 4 cut(s) 90, 187, 220, 374
BtsIMutI CAGTG 2 cut(s) 852, 889
Cac8I GCNNGC 1 cut(s) 486
CfoI GCGC 2 cut(s) 583, 1242
Cfr10I RCCGGY 2 cut(s) 929, 983
Cfr13I GGNCC 3 cut(s) 22, 686, 716
CseI GACGC 1 cut(s) 1095
Csp6I GTAC 2 cut(s) 625, 727
CspCI CAANNNNNGTGG 2 cut(s) 841, 876
CviAII CATG 1 cut(s) 995
CviQI GTAC 2 cut(s) 625, 727
DdeI CTNAG 1 cut(s) 855
DpnI GATC 3 cut(s) 335, 469, 531
DpnII GATC 3 cut(s) 333, 467, 529
Eam1104I CTCTTC 3 cut(s) 159, 243, 690
EarI CTCTTC 3 cut(s) 159, 243, 690
EciI GGCGGA 1 cut(s) 186
Eco130I CCWWGG 1 cut(s) 1156
Eco47I GGWCC 2 cut(s) 22, 716
Eco72I CACGTG 1 cut(s) 1173
EcoRI GAATTC 1 cut(s) 898
EcoRII CCWGG 2 cut(s) 32, 157
EcoT14I CCWWGG 1 cut(s) 1156
ErhI CCWWGG 1 cut(s) 1156
FaeI CATG 1 cut(s) 998
FalI AAGNNNNNCTT 2 cut(s) 1055, 1087
FaqI GGGAC 3 cut(s) 8, 795, 1217
FatI CATG 1 cut(s) 994
FauI CCCGC 2 cut(s) 104, 157
FauNDI CATATG 1 cut(s) 243
FblI GTMKAC 5 cut(s) 523, 606, 846, 1097, 1258
Fnu4HI GCNGC 4 cut(s) 200, 203, 584, 1238
FokI GGATG 4 cut(s) 77, 194, 227, 381
Fsp4HI GCNGC 4 cut(s) 200, 203, 584, 1238
FspBI CTAG 2 cut(s) 653, 795
GlaI GCGC 2 cut(s) 582, 1241
GluI GCNGC 4 cut(s) 200, 203, 584, 1238
GsuI CTGGAG 2 cut(s) 141, 1248
HaeIII GGCC 2 cut(s) 283, 688
HapII CCGG 2 cut(s) 930, 984
HgaI GACGC 1 cut(s) 1095
HhaI GCGC 2 cut(s) 583, 1242
Hin1I GRCGYC 1 cut(s) 843
Hin1II CATG 1 cut(s) 998
Hin6I GCGC 2 cut(s) 581, 1240
HinP1I GCGC 2 cut(s) 581, 1240
HinfI GANTC 5 cut(s) 112, 193, 232, 1016, 1148
HpaII CCGG 2 cut(s) 930, 984
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 6 cut(s) 524, 607, 847, 1015, 1098, 1259
Hpy188I TCNGA 4 cut(s) 85, 109, 472, 1153
Hpy188III TCNNGA 3 cut(s) 236, 296, 429
Hpy8I GTNNAC 6 cut(s) 524, 607, 847, 1015, 1098, 1259
Hpy99I CGWCG 2 cut(s) 89, 310
HpyAV CCTTC 3 cut(s) 312, 916, 1225
HpyCH4III ACNGT 4 cut(s) 576, 786, 976, 1279
HpyCH4IV ACGT 6 cut(s) 308, 526, 757, 843, 1141, 1172
HpyCH4V TGCA 4 cut(s) 408, 457, 1057, 1170
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 289, 298, 463, 706, 926
HpyF3I CTNAG 1 cut(s) 855
HpySE526I ACGT 6 cut(s) 308, 526, 757, 843, 1141, 1172
Hsp92I GRCGYC 1 cut(s) 843
Hsp92II CATG 1 cut(s) 998
HspAI GCGC 2 cut(s) 581, 1240
Kzo9I GATC 3 cut(s) 333, 467, 529
LguI GCTCTTC 1 cut(s) 690
LmnI GCTCC 3 cut(s) 122, 238, 463
Lsp1109I GCAGC 2 cut(s) 595, 1224
LweI GCATC 1 cut(s) 487
MaeI CTAG 2 cut(s) 653, 795
MaeII ACGT 6 cut(s) 308, 526, 757, 843, 1141, 1172
MaeIII GTNAC 1 cut(s) 1142
MalI GATC 3 cut(s) 335, 469, 531
MboI GATC 3 cut(s) 333, 467, 529
MboII GAAGA 6 cut(s) 176, 241, 260, 582, 707, 1075
MluCI AATT 7 cut(s) 67, 362, 800, 898, 1024, 1162, 1207
MlyI GAGTC 3 cut(s) 202, 1010, 1157
MmeI TCCRAC 2 cut(s) 108, 165
MseI TTAA 3 cut(s) 345, 803, 1227
MspI CCGG 2 cut(s) 930, 984
MspR9I CCNGG 2 cut(s) 34, 159
MvaI CCWGG 2 cut(s) 34, 159
MwoI GCNNNNNNNGC 6 cut(s) 44, 289, 298, 463, 706, 926
NdeI CATATG 1 cut(s) 243
NdeII GATC 3 cut(s) 333, 467, 529
NlaIII CATG 1 cut(s) 998
NlaIV GGNNCC 3 cut(s) 24, 639, 1281
PciSI GCTCTTC 1 cut(s) 690
PcsI WCGNNNNNNNCGW 1 cut(s) 1025
PfeI GAWTC 2 cut(s) 112, 232
PfoI TCCNGGA 1 cut(s) 157
PkrI GCNGC 4 cut(s) 201, 204, 585, 1239
PleI GAGTC 3 cut(s) 201, 1010, 1156
PmaCI CACGTG 1 cut(s) 1173
PmlI CACGTG 1 cut(s) 1173
PpsI GAGTC 3 cut(s) 201, 1010, 1156
Ppu21I YACGTR 2 cut(s) 527, 1173
Psp1406I AACGTT 1 cut(s) 1141
Psp6I CCWGG 2 cut(s) 32, 157
PspCI CACGTG 1 cut(s) 1173
PspGI CCWGG 2 cut(s) 32, 157
PspN4I GGNNCC 3 cut(s) 24, 639, 1281
PspPI GGNCC 3 cut(s) 22, 686, 716
PstI CTGCAG 1 cut(s) 459
RsaI GTAC 2 cut(s) 626, 728
RsaNI GTAC 2 cut(s) 625, 727
SapI GCTCTTC 1 cut(s) 690
SaqAI TTAA 3 cut(s) 345, 803, 1227
SatI GCNGC 4 cut(s) 200, 203, 584, 1238
Sau3AI GATC 3 cut(s) 333, 467, 529
Sau96I GGNCC 3 cut(s) 22, 686, 716
ScaI AGTACT 1 cut(s) 626
SchI GAGTC 3 cut(s) 202, 1010, 1157
ScrFI CCNGG 2 cut(s) 34, 159
SfaNI GCATC 1 cut(s) 487
SfcI CTRYAG 1 cut(s) 455
SinI GGWCC 2 cut(s) 22, 716
SmlI CTYRAG 1 cut(s) 427
SmoI CTYRAG 1 cut(s) 427
Sse9I AATT 7 cut(s) 67, 362, 800, 898, 1024, 1162, 1207
SsiI CCGC 6 cut(s) 97, 126, 150, 197, 200, 203
SspMI CTAG 2 cut(s) 653, 795
StyD4I CCNGG 2 cut(s) 32, 157
StyI CCWWGG 1 cut(s) 1156
TaaI ACNGT 4 cut(s) 576, 786, 976, 1279
TaiI ACGT 6 cut(s) 311, 529, 760, 846, 1144, 1175
TaqI TCGA 3 cut(s) 267, 305, 1019
TasI AATT 7 cut(s) 67, 362, 800, 898, 1024, 1162, 1207
TatI WGTACW 2 cut(s) 624, 726
TauI GCSGC 2 cut(s) 202, 205
TfiI GAWTC 2 cut(s) 112, 232
Tru1I TTAA 3 cut(s) 345, 803, 1227
Tru9I TTAA 3 cut(s) 345, 803, 1227
TscAI CASTG 2 cut(s) 859, 889
TseI GCWGC 2 cut(s) 583, 1237
TspDTI ATGAA 1 cut(s) 1200
TspGWI ACGGA 1 cut(s) 26
TspRI CASTG 2 cut(s) 859, 889
VpaK11BI GGWCC 2 cut(s) 22, 716
XapI RAATTY 3 cut(s) 898, 1024, 1162
XcmI CCANNNNNNNNNTGG 1 cut(s) 16
XmiI GTMKAC 5 cut(s) 523, 606, 846, 1097, 1258
XspI CTAG 2 cut(s) 653, 795
ZraI GACGTC 1 cut(s) 844
ZrmI AGTACT 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.