Rroxscaffold_6G00403900

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001599
Physical Location & Seq
Forward (+)
26360643 .. 26362957
2315 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00403900.1

Sequence Viewer

Length: 1050 bp
ATGGTAGTATTGGTTAGAGACTTTGATAGTGGGTTACTTGTGTCTTTATCACGCCAGCAAAGGAAGCCATTCTTAATAATGGTACGGTTGTTGGGCCATTGCTATTGCGACGGTTCTATCTGGCGTCGCCCTTTCAAAGACATAAACTTCGGCCCTATTCGATTCATCGATGACAGTTCCGTCATCTTCAAAGACATTACTTACGATCAAAACCTCAACCTCTCCCTCCGTTTATACAAACCAAGACTAACAAACCCTACTTCCTCAGCTGCCAAGCTCCCGGTGGTGCTCTACTTTCACGGCGGCGGTTTTTGCGGCGGCTCACGTGTCTGGCCCCACTACCGCAACTTATGTGTCAGATTGGCAGCCGAGCTCAAGGCCCTTGTGGTGTCACCGGACCACCGGCTTGCGCCGGAGGATAAGCTCCCTGCTGCGCTGGACGATGCGGCGTGTGTGGTGGATTGGCTGCAAAGGGAGGCCTTGATGAGTGAGAGTGGTGGTGACTGTGATGCATGGATACGTGGTGGTGTCGTTGACTTTGATAGGGTTTTTATCGTGGGTGATTCTTCCGGTGGCACCATAGCTCACCACCTGGCGGTTTCGCTCGGAGGCGGATCAGTCAAGGCGGCGCCGCTTCGGGTACGGGGGTACGTGTTGGTTGCGCCATTTTTTGGTGGAGTGGAGTGGACAAAATCAGAGCAGGAGCCTCATGAGCCACGGCTGACATTGGATGTAGTTGAAAGACTTTGGAGGCTTTCCCTGCCAGAAGGAGCAACCAGGGATCATCCGCTGGCAAACCCATTTGGACCAAACAGTCCACGCCTTGAGACGGTGGATCTTGATCCCATCTTGGTTATTGTGGGTGGCGATGAAATGTTGAAAGATAGATCGGTGATGTACTGGAGAAAACTAAAAGAGTTGGGAAAGAAAATTGAATATGCGGAATTTGATGGAGAGCAACATGGGTTTTTCTCAATTAATCCATACTCGGATGTTGCAAATGAAGCATTCCACGTTGTTAAGAGATTTGTGATTGAAAACTCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

349

Amino Acids

39.05

Weight (kDa)

6.37

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 85 - 201 8e-10 BD-FAE
Abhydrolase_3 PF07859 96 - 324 2.2e-52 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 96 - 258 4.5e-06 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 179, 813
AccB1I GGYRCC 2 cut(s) 575, 628
AccB7I CCANNNNNTGG 1 cut(s) 671
AclWI GGATC 4 cut(s) 622, 789, 836, 843
AcsI RAATTY 1 cut(s) 944
AcvI CACGTG 1 cut(s) 326
AcyI GRCGYC 2 cut(s) 124, 629
AfaI GTAC 4 cut(s) 84, 642, 650, 899
AfiI CCNNNNNNNGG 3 cut(s) 595, 671, 829
AflIII ACRYGT 2 cut(s) 325, 651
AgsI TTSAA 6 cut(s) 136, 190, 740, 880, 935, 1037
AjnI CCWGG 2 cut(s) 591, 776
AluBI AGCT 5 cut(s) 269, 277, 373, 424, 584
AluI AGCT 5 cut(s) 269, 277, 373, 424, 584
Alw21I GWGCWC 2 cut(s) 291, 375
Alw26I GTCTC 2 cut(s) 12, 821
AlwI GGATC 4 cut(s) 622, 789, 836, 843
AoxI GGCC 5 cut(s) 94, 151, 332, 378, 477
ApeKI GCWGC 4 cut(s) 269, 365, 431, 466
ApoI RAATTY 1 cut(s) 944
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AseI ATTAAT 1 cut(s) 978
Asp700I GAANNNNTTC 1 cut(s) 68
AspLEI GCGC 4 cut(s) 412, 436, 631, 664
AspS9I GGNCC 6 cut(s) 94, 152, 333, 379, 397, 806
AsuC2I CCSGG 1 cut(s) 281
AsuHPI GGTGA 5 cut(s) 384, 512, 572, 578, 904
AvaII GGWCC 2 cut(s) 397, 806
BaeI ACNNNNGTAYC 4 cut(s) 74, 107, 632, 665
BanI GGYRCC 2 cut(s) 575, 628
BanII GRGCYC 1 cut(s) 375
BbrPI CACGTG 1 cut(s) 326
Bbv12I GWGCWC 2 cut(s) 291, 375
BbvCI CCTCAGC 1 cut(s) 265
BbvI GCAGC 4 cut(s) 256, 377, 418, 453
BccI CCATC 2 cut(s) 854, 944
BceAI ACGGC 2 cut(s) 316, 734
BciT130I CCWGG 2 cut(s) 593, 778
BciVI GTATCC 1 cut(s) 510
BcnI CCSGG 1 cut(s) 281
BcoDI GTCTC 2 cut(s) 12, 821
BfoI RGCGCY 1 cut(s) 632
BfuI GTATCC 1 cut(s) 510
Bme1390I CCNGG 3 cut(s) 281, 593, 778
Bme18I GGWCC 2 cut(s) 397, 806
BmgT120I GGNCC 6 cut(s) 94, 152, 333, 379, 397, 806
BmiI GGNNCC 4 cut(s) 335, 577, 630, 705
BmrFI CCNGG 3 cut(s) 281, 593, 778
BmsI GCATC 2 cut(s) 433, 499
BpmI CTGGAG 1 cut(s) 922
Bpu10I CCTNAGC 1 cut(s) 265
BpuEI CTTGAG 2 cut(s) 359, 845
BpuMI CCSGG 1 cut(s) 281
Bsa29I ATCGAT 1 cut(s) 168
BsaAI YACGTR 3 cut(s) 326, 521, 652
BsaBI GATNNNNATC 1 cut(s) 840
BsaHI GRCGYC 2 cut(s) 124, 629
BsaJI CCNNGG 2 cut(s) 716, 777
BsaWI WCCGGW 2 cut(s) 394, 569
Bsc4I CCNNNNNNNGG 3 cut(s) 595, 671, 829
Bse118I RCCGGY 1 cut(s) 402
Bse1I ACTGG 1 cut(s) 905
Bse3DI GCAATG 1 cut(s) 97
Bse8I GATNNNNATC 1 cut(s) 840
BseBI CCWGG 2 cut(s) 593, 778
BseCI ATCGAT 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 716, 777
BseGI GGATG 3 cut(s) 736, 784, 997
BseJI GATNNNNATC 1 cut(s) 840
BseLI CCNNNNNNNGG 3 cut(s) 595, 671, 829
BseMI GCAATG 1 cut(s) 97
BseMII CTCAG 1 cut(s) 279
BseNI ACTGG 1 cut(s) 905
BseXI GCAGC 4 cut(s) 256, 377, 418, 453
BshFI GGCC 5 cut(s) 96, 153, 334, 380, 479
BshNI GGYRCC 2 cut(s) 575, 628
BshVI ATCGAT 1 cut(s) 168
BsiHKAI GWGCWC 2 cut(s) 291, 375
BsiSI CCGG 5 cut(s) 281, 395, 403, 413, 570
BslI CCNNNNNNNGG 3 cut(s) 595, 671, 829
BsmAI GTCTC 2 cut(s) 12, 821
BsmBI CGTCTC 1 cut(s) 821
BsmI GAATGC 1 cut(s) 1007
BsnI GGCC 5 cut(s) 96, 153, 334, 380, 479
Bsp1286I GDGCHC 2 cut(s) 291, 375
Bsp143I GATC 6 cut(s) 205, 614, 781, 835, 841, 887
BspANI GGCC 5 cut(s) 96, 153, 334, 380, 479
BspCNI CTCAG 1 cut(s) 278
BspDI ATCGAT 1 cut(s) 168
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 4 cut(s) 335, 577, 630, 705
BspPI GGATC 4 cut(s) 622, 789, 836, 843
BspT107I GGYRCC 2 cut(s) 575, 628
BsrDI GCAATG 1 cut(s) 97
BsrFI RCCGGY 1 cut(s) 402
BsrI ACTGG 1 cut(s) 905
BssAI RCCGGY 1 cut(s) 402
BssECI CCNNGG 2 cut(s) 716, 777
BssMI GATC 6 cut(s) 205, 614, 781, 835, 841, 887
BssNI GRCGYC 2 cut(s) 124, 629
Bst2UI CCWGG 2 cut(s) 593, 778
Bst4CI ACNGT 6 cut(s) 87, 113, 176, 506, 815, 832
BstACI GRCGYC 2 cut(s) 124, 629
BstBAI YACGTR 3 cut(s) 326, 521, 652
BstC8I GCNNGC 3 cut(s) 56, 408, 792
BstDEI CTNAG 1 cut(s) 265
BstDSI CCRYGG 1 cut(s) 716
BstF5I GGATG 3 cut(s) 736, 784, 997
BstH2I RGCGCY 1 cut(s) 632
BstHHI GCGC 4 cut(s) 412, 436, 631, 664
BstKTI GATC 6 cut(s) 208, 617, 784, 838, 844, 890
BstMAI GTCTC 2 cut(s) 12, 821
BstMBI GATC 6 cut(s) 205, 614, 781, 835, 841, 887
BstMWI GCNNNNNNNGC 5 cut(s) 64, 312, 712, 760, 1004
BstNI CCWGG 2 cut(s) 593, 778
BstSCI CCNGG 3 cut(s) 279, 591, 776
BstV1I GCAGC 4 cut(s) 256, 377, 418, 453
BstX2I RGATCY 1 cut(s) 835
BstYI RGATCY 1 cut(s) 835
Bsu15I ATCGAT 1 cut(s) 168
BsuI GTATCC 1 cut(s) 510
BsuRI GGCC 5 cut(s) 96, 153, 334, 380, 479
BsuTUI ATCGAT 1 cut(s) 168
BtgI CCRYGG 1 cut(s) 716
BtgZI GCGATG 1 cut(s) 882
BtsCI GGATG 3 cut(s) 736, 784, 997
Cac8I GCNNGC 3 cut(s) 56, 408, 792
CciI TCATGA 1 cut(s) 709
CfoI GCGC 4 cut(s) 412, 436, 631, 664
Cfr10I RCCGGY 1 cut(s) 402
Cfr13I GGNCC 6 cut(s) 94, 152, 333, 379, 397, 806
ClaI ATCGAT 1 cut(s) 168
CseI GACGC 1 cut(s) 113
Csp6I GTAC 4 cut(s) 83, 641, 649, 898
CviAII CATG 3 cut(s) 513, 710, 962
CviQI GTAC 4 cut(s) 83, 641, 649, 898
DdeI CTNAG 1 cut(s) 265
DinI GGCGCC 1 cut(s) 630
DpnI GATC 6 cut(s) 207, 616, 783, 837, 843, 889
DpnII GATC 6 cut(s) 205, 614, 781, 835, 841, 887
DrdI GACNNNNNNGTC 2 cut(s) 179, 813
DseDI GACNNNNNNGTC 2 cut(s) 179, 813
EciI GGCGGA 1 cut(s) 627
Ecl136II GAGCTC 1 cut(s) 373
Eco147I AGGCCT 1 cut(s) 479
Eco24I GRGCYC 1 cut(s) 375
Eco47I GGWCC 2 cut(s) 397, 806
Eco53kI GAGCTC 1 cut(s) 373
Eco72I CACGTG 1 cut(s) 326
EcoICRI GAGCTC 1 cut(s) 373
EcoO109I RGGNCCY 1 cut(s) 379
EcoRII CCWGG 2 cut(s) 591, 776
EcoT22I ATGCAT 1 cut(s) 514
EcoT38I GRGCYC 1 cut(s) 375
EgeI GGCGCC 1 cut(s) 630
EheI GGCGCC 1 cut(s) 630
Esp3I CGTCTC 1 cut(s) 821
FaeI CATG 3 cut(s) 516, 713, 965
FaiI YATR 9 cut(s) 143, 235, 352, 514, 581, 711, 939, 963, 985
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FatI CATG 3 cut(s) 512, 709, 961
FokI GGATG 3 cut(s) 743, 771, 1004
FriOI GRGCYC 1 cut(s) 375
GlaI GCGC 4 cut(s) 411, 435, 630, 663
GsuI CTGGAG 1 cut(s) 922
HaeII RGCGCY 1 cut(s) 632
HaeIII GGCC 5 cut(s) 96, 153, 334, 380, 479
HapII CCGG 5 cut(s) 281, 395, 403, 413, 570
HgaI GACGC 1 cut(s) 113
HhaI GCGC 4 cut(s) 412, 436, 631, 664
Hin1I GRCGYC 2 cut(s) 124, 629
Hin1II CATG 3 cut(s) 516, 713, 965
Hin6I GCGC 4 cut(s) 410, 434, 629, 662
HinP1I GCGC 4 cut(s) 410, 434, 629, 662
HincII GTYRAC 1 cut(s) 535
HindII GTYRAC 1 cut(s) 535
HinfI GANTC 2 cut(s) 162, 563
HpaII CCGG 5 cut(s) 281, 395, 403, 413, 570
HphI GGTGA 5 cut(s) 384, 512, 572, 578, 904
Hpy166II GTNNAC 3 cut(s) 535, 687, 818
Hpy188I TCNGA 4 cut(s) 359, 608, 697, 991
Hpy188III TCNNGA 2 cut(s) 710, 839
Hpy8I GTNNAC 3 cut(s) 535, 687, 818
Hpy99I CGWCG 2 cut(s) 113, 129
HpyAV CCTTC 1 cut(s) 761
HpyCH4III ACNGT 6 cut(s) 87, 113, 176, 506, 815, 832
HpyCH4IV ACGT 4 cut(s) 325, 520, 651, 1014
HpyCH4V TGCA 3 cut(s) 469, 512, 998
HpyF10VI GCNNNNNNNGC 5 cut(s) 64, 312, 712, 760, 1004
HpyF3I CTNAG 1 cut(s) 265
HpySE526I ACGT 4 cut(s) 325, 520, 651, 1014
Hsp92I GRCGYC 2 cut(s) 124, 629
Hsp92II CATG 3 cut(s) 516, 713, 965
HspAI GCGC 4 cut(s) 410, 434, 629, 662
KasI GGCGCC 1 cut(s) 628
Kzo9I GATC 6 cut(s) 205, 614, 781, 835, 841, 887
LmnI GCTCC 4 cut(s) 282, 429, 703, 770
Lsp1109I GCAGC 4 cut(s) 256, 377, 418, 453
LweI GCATC 2 cut(s) 433, 499
MaeII ACGT 4 cut(s) 325, 520, 651, 1014
MaeIII GTNAC 3 cut(s) 33, 390, 500
MalI GATC 6 cut(s) 207, 616, 783, 837, 843, 889
MboI GATC 6 cut(s) 205, 614, 781, 835, 841, 887
MboII GAAGA 2 cut(s) 178, 558
MflI RGATCY 1 cut(s) 835
MhlI GDGCHC 2 cut(s) 291, 375
MluCI AATT 3 cut(s) 930, 944, 975
Mly113I GGCGCC 1 cut(s) 629
MnlI CCTC 9 cut(s) 224, 230, 236, 274, 409, 469, 602, 717, 744
Mph1103I ATGCAT 1 cut(s) 514
MroXI GAANNNNTTC 1 cut(s) 68
MseI TTAA 3 cut(s) 74, 978, 1020
MspA1I CMGCKG 2 cut(s) 269, 790
MspI CCGG 5 cut(s) 281, 395, 403, 413, 570
MspR9I CCNGG 3 cut(s) 281, 593, 778
Mva1269I GAATGC 1 cut(s) 1007
MvaI CCWGG 2 cut(s) 593, 778
MwoI GCNNNNNNNGC 5 cut(s) 64, 312, 712, 760, 1004
NarI GGCGCC 1 cut(s) 629
NciI CCSGG 1 cut(s) 281
NdeII GATC 6 cut(s) 205, 614, 781, 835, 841, 887
NlaIII CATG 3 cut(s) 516, 713, 965
NlaIV GGNNCC 4 cut(s) 335, 577, 630, 705
NmeAIII GCCGAG 1 cut(s) 394
NmuCI GTSAC 2 cut(s) 390, 500
NsiI ATGCAT 1 cut(s) 514
PagI TCATGA 1 cut(s) 709
PceI AGGCCT 1 cut(s) 479
PctI GAATGC 1 cut(s) 1007
PdmI GAANNNNTTC 1 cut(s) 68
PfeI GAWTC 2 cut(s) 162, 563
PflMI CCANNNNNTGG 1 cut(s) 671
PluTI GGCGCC 1 cut(s) 632
PmaCI CACGTG 1 cut(s) 326
PmlI CACGTG 1 cut(s) 326
Ppu21I YACGTR 3 cut(s) 326, 521, 652
PshBI ATTAAT 1 cut(s) 978
Psp124BI GAGCTC 1 cut(s) 375
Psp6I CCWGG 2 cut(s) 591, 776
PspCI CACGTG 1 cut(s) 326
PspGI CCWGG 2 cut(s) 591, 776
PspN4I GGNNCC 4 cut(s) 335, 577, 630, 705
PspPI GGNCC 6 cut(s) 94, 152, 333, 379, 397, 806
PsuI RGATCY 1 cut(s) 835
PvuII CAGCTG 1 cut(s) 269
RsaI GTAC 4 cut(s) 84, 642, 650, 899
RsaNI GTAC 4 cut(s) 83, 641, 649, 898
SacI GAGCTC 1 cut(s) 375
SaqAI TTAA 3 cut(s) 74, 978, 1020
Sau3AI GATC 6 cut(s) 205, 614, 781, 835, 841, 887
Sau96I GGNCC 6 cut(s) 94, 152, 333, 379, 397, 806
ScrFI CCNGG 3 cut(s) 281, 593, 778
SduI GDGCHC 2 cut(s) 291, 375
SfaNI GCATC 2 cut(s) 433, 499
SfoI GGCGCC 1 cut(s) 630
SinI GGWCC 2 cut(s) 397, 806
SmlI CTYRAG 2 cut(s) 374, 824
SmoI CTYRAG 2 cut(s) 374, 824
Sse9I AATT 3 cut(s) 930, 944, 975
SseBI AGGCCT 1 cut(s) 479
SspDI GGCGCC 1 cut(s) 628
SstI GAGCTC 1 cut(s) 375
StuI AGGCCT 1 cut(s) 479
StyD4I CCNGG 3 cut(s) 279, 591, 776
TaaI ACNGT 6 cut(s) 87, 113, 176, 506, 815, 832
TaiI ACGT 4 cut(s) 328, 523, 654, 1017
TaqI TCGA 2 cut(s) 160, 168
TasI AATT 3 cut(s) 930, 944, 975
TatI WGTACW 1 cut(s) 897
TauI GCSGC 6 cut(s) 306, 318, 321, 449, 629, 634
TfiI GAWTC 2 cut(s) 162, 563
Tru1I TTAA 3 cut(s) 74, 978, 1020
Tru9I TTAA 3 cut(s) 74, 978, 1020
TseFI GTSAC 2 cut(s) 390, 500
TseI GCWGC 4 cut(s) 269, 365, 431, 466
Tsp45I GTSAC 2 cut(s) 390, 500
TspDTI ATGAA 3 cut(s) 154, 885, 1017
TspGWI ACGGA 2 cut(s) 169, 218
Van91I CCANNNNNTGG 1 cut(s) 671
VpaK11BI GGWCC 2 cut(s) 397, 806
VspI ATTAAT 1 cut(s) 978
XapI RAATTY 1 cut(s) 944
XcmI CCANNNNNNNNNTGG 1 cut(s) 280
XmnI GAANNNNTTC 1 cut(s) 68
Zsp2I ATGCAT 1 cut(s) 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.