Rh3BG245500

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001599
Physical Location & Seq
Reverse (-)
23272784 .. 23275659
2876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG245500.1

Sequence Viewer

Length: 993 bp
ATGGGTTCCCTCACTCACATTGTGGAAGACTGCATGGGCGTTCTCCAACTCTTTAGCGACGGTTCCGTCCATCGCTGCTCTTTGAAAGACATAAACTTCCCGTTTCCAGCCGTTAACGACAACTCCGTGGACTTCAAAGACGTCGTTTTCGACAGAAAGCTCAACATTTCCCTACGTGTGTACAGACCTAAAAGCCATGCGTCCCCGACGCCCGCAAACCTTCCCGTGGTGTTTTACTTCCACGGGGGCGGTTTCTGCGTTGGCTCGCGGGTGTGGCCCAACTGCCACAACTGCTGCATCCGCCTGGCATCTGAGCTCAACGCGCTCGTCGTGGCGCCAGACTACCGGCTCGCCCCCGAGCACAAGCTCCCGGCAGCGGTGGATGACGCGTTGACTGCGGTGGAGTGGCTGCAGAGGGAGGCTTTGATGAGTGAGGGCAGTGGTGATTGTGATGCATGGCTGGGTGGTGGTATGGTTGATTTTGACCGGGTTTATGTGGTGGGCGACTCATCAGGAGGTAATATGGCTCACCACGTGGCCGTACGGCTCGGAGCCGGGTCTGTCAAGGTGGCGCCGGTGCGGGTACGCGGATATGTGCTTTTGGCGCCATTTTTTGGTGGAGTGGAGAGGACAAAGTCGGAGGAGGGTCCGTCGGAGACGTTTTTGAATTTGGAGATACTTGACAGATTTTGGAGGCTGTCATTGCCAAAAGGAGAAACCAGGGATCATCCAATGGCCAACCCATTTGGACCAAACAGCCCCAGCCTTGAGGAGGTGGATCTTGATCCCATCTTGGTTATTGCGGGTGGCAATGAAATGTTGAAAGATAGAGTGGAGATCTACTCGAGGAGACTGAAAGAGTTGGGAAAGAAGATTGAGTATGTGGATTTTCATGGACAACAACATGGCTTTCTCACAAATGATCCATACTCAGAAGTGGCAAATGAAGCACTACAAGTTATTAAGAGATTTATGATTGAAAACTCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

330

Amino Acids

36.51

Weight (kDa)

5.57

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 58 - 118 1.4e-06 Carboxylesterase family
BD-FAE PF20434 60 - 181 6.1e-10 BD-FAE
Abhydrolase_3 PF07859 77 - 305 1.2e-57 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 65
AatII GACGTC 1 cut(s) 144
AccB1I GGYRCC 3 cut(s) 334, 571, 604
AccB7I CCANNNNNTGG 1 cut(s) 614
AccII CGCG 4 cut(s) 268, 323, 389, 588
AciI CCGC 9 cut(s) 213, 249, 268, 301, 377, 398, 580, 588, 803
AclWI GGATC 4 cut(s) 732, 779, 786, 917
AcoI YGGCCR 2 cut(s) 537, 735
AcsI RAATTY 1 cut(s) 667
AcvI CACGTG 1 cut(s) 535
AcyI GRCGYC 5 cut(s) 141, 209, 335, 572, 605
AdeI CACNNNGTG 2 cut(s) 22, 535
AfaI GTAC 3 cut(s) 182, 543, 585
AfiI CCNNNNNNNGG 4 cut(s) 226, 376, 614, 772
AflIII ACRYGT 2 cut(s) 175, 387
AgsI TTSAA 5 cut(s) 85, 136, 667, 823, 980
AjnI CCWGG 2 cut(s) 303, 719
AluBI AGCT 3 cut(s) 160, 316, 367
AluI AGCT 3 cut(s) 160, 316, 367
Alw21I GWGCWC 2 cut(s) 318, 363
Alw26I GTCTC 2 cut(s) 650, 844
AlwI GGATC 4 cut(s) 732, 779, 786, 917
Ama87I CYCGRG 2 cut(s) 356, 844
AoxI GGCC 3 cut(s) 275, 537, 735
ApeKI GCWGC 4 cut(s) 75, 294, 374, 409
ApoI RAATTY 1 cut(s) 667
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AspLEI GCGC 4 cut(s) 325, 337, 574, 607
AspS9I GGNCC 3 cut(s) 276, 647, 749
AsuC2I CCSGG 3 cut(s) 371, 488, 556
AsuHPI GGTGA 2 cut(s) 455, 521
AvaI CYCGRG 2 cut(s) 356, 844
AvaII GGWCC 2 cut(s) 647, 749
BalI TGGCCA 1 cut(s) 737
BanI GGYRCC 3 cut(s) 334, 571, 604
BanII GRGCYC 1 cut(s) 318
BbrPI CACGTG 1 cut(s) 535
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 2 cut(s) 318, 363
BbvI GCAGC 4 cut(s) 62, 281, 386, 396
BccI CCATC 2 cut(s) 78, 797
BceAI ACGGC 3 cut(s) 95, 524, 560
BciT130I CCWGG 2 cut(s) 305, 721
BcnI CCSGG 3 cut(s) 371, 488, 556
BcoDI GTCTC 2 cut(s) 650, 844
BfmI CTRYAG 1 cut(s) 410
BfoI RGCGCY 3 cut(s) 338, 575, 608
BglII AGATCT 1 cut(s) 837
BisI GCNGC 4 cut(s) 76, 295, 375, 410
BlsI GCNGC 4 cut(s) 77, 296, 376, 411
Bme1390I CCNGG 5 cut(s) 305, 371, 488, 556, 721
Bme18I GGWCC 2 cut(s) 647, 749
BmeT110I CYCGRG 2 cut(s) 356, 844
BmgT120I GGNCC 3 cut(s) 276, 647, 749
BmiI GGNNCC 7 cut(s) 7, 64, 336, 553, 573, 606, 648
BmrFI CCNGG 5 cut(s) 305, 371, 488, 556, 721
BmsI GCATC 3 cut(s) 306, 317, 442
BpiI GAAGAC 1 cut(s) 33
BplI GAGNNNNNCTC 2 cut(s) 827, 859
BpuEI CTTGAG 1 cut(s) 788
BpuMI CCSGG 3 cut(s) 371, 488, 556
BsaAI YACGTR 2 cut(s) 176, 535
BsaBI GATNNNNATC 1 cut(s) 783
BsaHI GRCGYC 5 cut(s) 141, 209, 335, 572, 605
BsaJI CCNNGG 4 cut(s) 126, 225, 241, 720
BsaXI ACNNNNNCTCC 4 cut(s) 107, 137, 635, 665
Bsc4I CCNNNNNNNGG 4 cut(s) 226, 376, 614, 772
Bse118I RCCGGY 2 cut(s) 345, 574
Bse3DI GCAATG 2 cut(s) 701, 817
Bse8I GATNNNNATC 1 cut(s) 783
BseBI CCWGG 2 cut(s) 305, 721
BseDI CCNNGG 4 cut(s) 126, 225, 241, 720
BseGI GGATG 3 cut(s) 297, 388, 727
BseJI GATNNNNATC 1 cut(s) 783
BseLI CCNNNNNNNGG 4 cut(s) 226, 376, 614, 772
BseMI GCAATG 2 cut(s) 701, 817
BseMII CTCAG 2 cut(s) 303, 945
BseRI GAGGAG 3 cut(s) 656, 785, 862
BseXI GCAGC 4 cut(s) 62, 281, 386, 396
BseYI CCCAGC 2 cut(s) 460, 761
Bsh1236I CGCG 4 cut(s) 268, 323, 389, 588
BshFI GGCC 3 cut(s) 277, 539, 737
BshNI GGYRCC 3 cut(s) 334, 571, 604
BsiHKAI GWGCWC 2 cut(s) 318, 363
BsiHKCI CYCGRG 2 cut(s) 356, 844
BsiSI CCGG 5 cut(s) 346, 371, 487, 555, 575
BsiWI CGTACG 1 cut(s) 541
BslFI GGGAC 1 cut(s) 187
BslI CCNNNNNNNGG 4 cut(s) 226, 376, 614, 772
BsmAI GTCTC 2 cut(s) 650, 844
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 1 cut(s) 187
BsnI GGCC 3 cut(s) 277, 539, 737
BsoBI CYCGRG 2 cut(s) 356, 844
Bsp1286I GDGCHC 2 cut(s) 318, 363
Bsp1407I TGTACA 1 cut(s) 180
Bsp143I GATC 5 cut(s) 724, 778, 784, 837, 922
BspACI CCGC 9 cut(s) 213, 249, 268, 301, 377, 398, 580, 588, 803
BspANI GGCC 3 cut(s) 277, 539, 737
BspCNI CTCAG 2 cut(s) 304, 944
BspFNI CGCG 4 cut(s) 268, 323, 389, 588
BspLI GGNNCC 7 cut(s) 7, 64, 336, 553, 573, 606, 648
BspMAI CTGCAG 1 cut(s) 414
BspPI GGATC 4 cut(s) 732, 779, 786, 917
BspT107I GGYRCC 3 cut(s) 334, 571, 604
BsrDI GCAATG 2 cut(s) 701, 817
BsrFI RCCGGY 2 cut(s) 345, 574
BsrGI TGTACA 1 cut(s) 180
BssAI RCCGGY 2 cut(s) 345, 574
BssECI CCNNGG 4 cut(s) 126, 225, 241, 720
BssMI GATC 5 cut(s) 724, 778, 784, 837, 922
BssNI GRCGYC 5 cut(s) 141, 209, 335, 572, 605
Bst2UI CCWGG 2 cut(s) 305, 721
Bst4CI ACNGT 1 cut(s) 62
BstACI GRCGYC 5 cut(s) 141, 209, 335, 572, 605
BstAUI TGTACA 1 cut(s) 180
BstBAI YACGTR 2 cut(s) 176, 535
BstC8I GCNNGC 3 cut(s) 213, 266, 351
BstDEI CTNAG 2 cut(s) 312, 931
BstDSI CCRYGG 3 cut(s) 126, 225, 241
BstENI CCTNNNNNAGG 1 cut(s) 770
BstF5I GGATG 3 cut(s) 297, 388, 727
BstFNI CGCG 4 cut(s) 268, 323, 389, 588
BstH2I RGCGCY 3 cut(s) 338, 575, 608
BstHHI GCGC 4 cut(s) 325, 337, 574, 607
BstKTI GATC 5 cut(s) 727, 781, 787, 840, 925
BstMAI GTCTC 2 cut(s) 650, 844
BstMBI GATC 5 cut(s) 724, 778, 784, 837, 922
BstMWI GCNNNNNNNGC 9 cut(s) 255, 274, 291, 300, 322, 395, 604, 703, 947
BstNI CCWGG 2 cut(s) 305, 721
BstSCI CCNGG 5 cut(s) 303, 369, 486, 554, 719
BstSFI CTRYAG 1 cut(s) 410
BstUI CGCG 4 cut(s) 268, 323, 389, 588
BstV1I GCAGC 4 cut(s) 62, 281, 386, 396
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 2 cut(s) 778, 837
BstYI RGATCY 2 cut(s) 778, 837
BsuRI GGCC 3 cut(s) 277, 539, 737
BtgI CCRYGG 3 cut(s) 126, 225, 241
BtgZI GCGATG 1 cut(s) 56
BtsCI GGATG 3 cut(s) 297, 388, 727
BtsI GCAGTG 1 cut(s) 445
BtsIMutI CAGTG 1 cut(s) 445
Cac8I GCNNGC 3 cut(s) 213, 266, 351
CfoI GCGC 4 cut(s) 325, 337, 574, 607
Cfr10I RCCGGY 2 cut(s) 345, 574
Cfr13I GGNCC 3 cut(s) 276, 647, 749
CseI GACGC 3 cut(s) 189, 217, 395
Csp6I GTAC 3 cut(s) 181, 542, 584
CviAII CATG 5 cut(s) 34, 197, 456, 893, 905
CviQI GTAC 3 cut(s) 181, 542, 584
DdeI CTNAG 2 cut(s) 312, 931
DinI GGCGCC 3 cut(s) 336, 573, 606
DpnI GATC 5 cut(s) 726, 780, 786, 839, 924
DpnII GATC 5 cut(s) 724, 778, 784, 837, 922
DraIII CACNNNGTG 2 cut(s) 22, 535
DrdI GACNNNNNNGTC 1 cut(s) 65
DseDI GACNNNNNNGTC 1 cut(s) 65
EaeI YGGCCR 2 cut(s) 537, 735
EciI GGCGGA 1 cut(s) 290
Ecl136II GAGCTC 1 cut(s) 316
Eco24I GRGCYC 1 cut(s) 318
Eco47I GGWCC 2 cut(s) 647, 749
Eco53kI GAGCTC 1 cut(s) 316
Eco72I CACGTG 1 cut(s) 535
Eco88I CYCGRG 2 cut(s) 356, 844
EcoICRI GAGCTC 1 cut(s) 316
EcoNI CCTNNNNNAGG 1 cut(s) 770
EcoRII CCWGG 2 cut(s) 303, 719
EcoT22I ATGCAT 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 318
EgeI GGCGCC 3 cut(s) 336, 573, 606
EheI GGCGCC 3 cut(s) 336, 573, 606
Esp3I CGTCTC 1 cut(s) 650
FaeI CATG 5 cut(s) 37, 200, 459, 896, 908
FaqI GGGAC 1 cut(s) 187
FatI CATG 5 cut(s) 33, 196, 455, 892, 904
FauI CCCGC 4 cut(s) 220, 261, 573, 796
Fnu4HI GCNGC 4 cut(s) 76, 295, 375, 410
FokI GGATG 3 cut(s) 284, 395, 714
FriOI GRGCYC 1 cut(s) 318
Fsp4HI GCNGC 4 cut(s) 76, 295, 375, 410
GlaI GCGC 4 cut(s) 324, 336, 573, 606
GluI GCNGC 4 cut(s) 76, 295, 375, 410
GsaI CCCAGC 2 cut(s) 464, 765
HaeII RGCGCY 3 cut(s) 338, 575, 608
HaeIII GGCC 3 cut(s) 277, 539, 737
HapII CCGG 5 cut(s) 346, 371, 487, 555, 575
HgaI GACGC 3 cut(s) 189, 217, 395
HhaI GCGC 4 cut(s) 325, 337, 574, 607
Hin1I GRCGYC 5 cut(s) 141, 209, 335, 572, 605
Hin1II CATG 5 cut(s) 37, 200, 459, 896, 908
Hin6I GCGC 4 cut(s) 323, 335, 572, 605
HinP1I GCGC 4 cut(s) 323, 335, 572, 605
HincII GTYRAC 2 cut(s) 115, 393
HindII GTYRAC 2 cut(s) 115, 393
HinfI GANTC 1 cut(s) 506
HpaI GTTAAC 1 cut(s) 115
HpaII CCGG 5 cut(s) 346, 371, 487, 555, 575
HphI GGTGA 2 cut(s) 455, 521
Hpy166II GTNNAC 4 cut(s) 115, 130, 181, 393
Hpy188I TCNGA 5 cut(s) 313, 551, 640, 655, 934
Hpy188III TCNNGA 2 cut(s) 513, 782
Hpy8I GTNNAC 4 cut(s) 115, 130, 181, 393
Hpy99I CGWCG 5 cut(s) 62, 146, 211, 332, 655
HpyAV CCTTC 1 cut(s) 230
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4IV ACGT 4 cut(s) 141, 175, 534, 659
HpyCH4V TGCA 4 cut(s) 33, 297, 412, 455
HpyF10VI GCNNNNNNNGC 9 cut(s) 255, 274, 291, 300, 322, 395, 604, 703, 947
HpyF3I CTNAG 2 cut(s) 312, 931
HpySE526I ACGT 4 cut(s) 141, 175, 534, 659
Hsp92I GRCGYC 5 cut(s) 141, 209, 335, 572, 605
Hsp92II CATG 5 cut(s) 37, 200, 459, 896, 908
HspAI GCGC 4 cut(s) 323, 335, 572, 605
KasI GGCGCC 3 cut(s) 334, 571, 604
KspAI GTTAAC 1 cut(s) 115
Kzo9I GATC 5 cut(s) 724, 778, 784, 837, 922
LmnI GCTCC 2 cut(s) 372, 551
Lsp1109I GCAGC 4 cut(s) 62, 281, 386, 396
LweI GCATC 3 cut(s) 306, 317, 442
MaeII ACGT 4 cut(s) 141, 175, 534, 659
MalI GATC 5 cut(s) 726, 780, 786, 839, 924
MboI GATC 5 cut(s) 724, 778, 784, 837, 922
MboII GAAGA 2 cut(s) 38, 883
MflI RGATCY 2 cut(s) 778, 837
MhlI GDGCHC 2 cut(s) 318, 363
MlsI TGGCCA 1 cut(s) 737
MluCI AATT 1 cut(s) 667
MluI ACGCGT 1 cut(s) 387
MluNI TGGCCA 1 cut(s) 737
Mly113I GGCGCC 3 cut(s) 335, 572, 605
MlyI GAGTC 1 cut(s) 500
MmeI TCCRAC 3 cut(s) 70, 618, 633
Mox20I TGGCCA 1 cut(s) 737
Mph1103I ATGCAT 1 cut(s) 457
MscI TGGCCA 1 cut(s) 737
MseI TTAA 2 cut(s) 114, 963
Msp20I TGGCCA 1 cut(s) 737
MspA1I CMGCKG 1 cut(s) 377
MspI CCGG 5 cut(s) 346, 371, 487, 555, 575
MspR9I CCNGG 5 cut(s) 305, 371, 488, 556, 721
MvaI CCWGG 2 cut(s) 305, 721
MvnI CGCG 4 cut(s) 268, 323, 389, 588
MwoI GCNNNNNNNGC 9 cut(s) 255, 274, 291, 300, 322, 395, 604, 703, 947
NarI GGCGCC 3 cut(s) 335, 572, 605
NciI CCSGG 3 cut(s) 371, 488, 556
NdeII GATC 5 cut(s) 724, 778, 784, 837, 922
NlaIII CATG 5 cut(s) 37, 200, 459, 896, 908
NlaIV GGNNCC 7 cut(s) 7, 64, 336, 553, 573, 606, 648
NsiI ATGCAT 1 cut(s) 457
PaeR7I CTCGAG 1 cut(s) 844
PcsI WCGNNNNNNNCGW 3 cut(s) 123, 147, 327
Pfl23II CGTACG 1 cut(s) 541
PflFI GACNNNGTC 1 cut(s) 634
PflMI CCANNNNNTGG 1 cut(s) 614
PkrI GCNGC 4 cut(s) 77, 296, 376, 411
PleI GAGTC 1 cut(s) 500
PluTI GGCGCC 3 cut(s) 338, 575, 608
PmaCI CACGTG 1 cut(s) 535
PmlI CACGTG 1 cut(s) 535
PpsI GAGTC 1 cut(s) 500
Ppu21I YACGTR 2 cut(s) 176, 535
Psp124BI GAGCTC 1 cut(s) 318
Psp6I CCWGG 2 cut(s) 303, 719
PspCI CACGTG 1 cut(s) 535
PspFI CCCAGC 2 cut(s) 460, 761
PspGI CCWGG 2 cut(s) 303, 719
PspLI CGTACG 1 cut(s) 541
PspN4I GGNNCC 7 cut(s) 7, 64, 336, 553, 573, 606, 648
PspPI GGNCC 3 cut(s) 276, 647, 749
PspXI VCTCGAGB 1 cut(s) 844
PstI CTGCAG 1 cut(s) 414
PsuI RGATCY 2 cut(s) 778, 837
PsyI GACNNNGTC 1 cut(s) 634
RsaI GTAC 3 cut(s) 182, 543, 585
RsaNI GTAC 3 cut(s) 181, 542, 584
SacI GAGCTC 1 cut(s) 318
SaqAI TTAA 2 cut(s) 114, 963
SatI GCNGC 4 cut(s) 76, 295, 375, 410
Sau3AI GATC 5 cut(s) 724, 778, 784, 837, 922
Sau96I GGNCC 3 cut(s) 276, 647, 749
SchI GAGTC 1 cut(s) 500
ScrFI CCNGG 5 cut(s) 305, 371, 488, 556, 721
SduI GDGCHC 2 cut(s) 318, 363
SfaNI GCATC 3 cut(s) 306, 317, 442
SfcI CTRYAG 1 cut(s) 410
SfoI GGCGCC 3 cut(s) 336, 573, 606
Sfr274I CTCGAG 1 cut(s) 844
SgrAI CRCCGGYG 1 cut(s) 574
SinI GGWCC 2 cut(s) 647, 749
SlaI CTCGAG 1 cut(s) 844
SmlI CTYRAG 2 cut(s) 767, 844
SmoI CTYRAG 2 cut(s) 767, 844
Sse9I AATT 1 cut(s) 667
SsiI CCGC 9 cut(s) 213, 249, 268, 301, 377, 398, 580, 588, 803
SspDI GGCGCC 3 cut(s) 334, 571, 604
SstI GAGCTC 1 cut(s) 318
StyD4I CCNGG 5 cut(s) 303, 369, 486, 554, 719
TaaI ACNGT 1 cut(s) 62
TaiI ACGT 4 cut(s) 144, 178, 537, 662
TaqI TCGA 2 cut(s) 150, 845
TasI AATT 1 cut(s) 667
TatI WGTACW 1 cut(s) 180
Tru1I TTAA 2 cut(s) 114, 963
Tru9I TTAA 2 cut(s) 114, 963
TscAI CASTG 1 cut(s) 445
TseI GCWGC 4 cut(s) 75, 294, 374, 409
TspDTI ATGAA 3 cut(s) 828, 881, 960
TspGWI ACGGA 3 cut(s) 55, 115, 639
TspRI CASTG 1 cut(s) 445
Tth111I GACNNNGTC 1 cut(s) 634
Van91I CCANNNNNTGG 1 cut(s) 614
VpaK11BI GGWCC 2 cut(s) 647, 749
XagI CCTNNNNNAGG 1 cut(s) 770
XapI RAATTY 1 cut(s) 667
XhoI CTCGAG 1 cut(s) 844
ZraI GACGTC 1 cut(s) 142
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.