pycom11g15080

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001599
Physical Location & Seq
Reverse (-)
15538386 .. 15539949
1564 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g15080.2

Sequence Viewer

Length: 1005 bp
ATGTCATCAAACACCGCCATCACTCCAACCTTGCCCTACGAAGAGTGCCGCGGTGTTCTCCGCGTCTACAGCGACGGCTCCATCGTCCGCTCTCCCAAACCAAGCTTCGACGTCCCAGTCCACGACGACGGCTCCGTTGACTGGAAAGACGTCGTTTTCGACCCAACCAATCAACTCCAACTCCGCCTCTACAAGCCTGCTGCAACTACACACTCCCCCTCGTCGTTGTCCAAAAAGCTCCCCATCTTCTACTACATCCACGGCGGCGGCTTCTGCATCGGCTCGCGCGCTTGGCCCAACTGCCAGAACTACTGTTTTCAGCTCGCCTCCCAGCTCCAATGCGTCGTCGTTGCACCTGACTATCGCCTCGCGCCAGAACACCGCCTCCCGGCTGCTATTGACGACGGCTTCGCGGCTATGAAATGGCTCCAAGCCCAAGCCGAGGCTGAGGATCCGGACACGTGGCTGACTGAGGTGGCGGATTTTGGGAACGTGTTTGTTTCGGGTGACTCGGCTGGCGGGAATATTGCTCATAACTTGGCGGTTCAGCTCGGGGCCGGTTCGGTTGAATTGGGGCCGGTTCGGGTGAGAGGGTACGTACTTTTGGCACCGTTTTTTGGTGGGACGGTTTTGGCTAGGTCAGAGGCTGAGGGCCCCAAAGAAGCCTTTCTCAATTGGGAGCTCATTGACAGGTTTTGGAGGCTGTCAATACCAATTGGAGAGGACAGAGATCACCCACTTGTGAACCCATTTGGACCAAATAGCCAGAGCCTAGAAGAAGTGGCTTTTGATCCAATCCTAGTGGTTGTAGGTGGAAGCGATCTTCTCAAAGATCGAGCTCAAGATTATGCAAATAGGCTCAAAAATTGGGGAAACAAGGTTGAGTACGTGGAATTTGAAGGACAGCAGCATGGTTTCTTCACCATCCAACCCAGTTCCCAACCTGCAAAGGAATTGATGTTAATCATCAAACGATTCATTGCTCAAAATTCCACTTGCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

335

Amino Acids

36.88

Weight (kDa)

5.12

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 116
AatII GACGTC 2 cut(s) 114, 153
Acc36I ACCTGC 1 cut(s) 952
AccB1I GGYRCC 1 cut(s) 607
AccBSI CCGCTC 1 cut(s) 90
AccI GTMKAC 1 cut(s) 66
AccII CGCG 6 cut(s) 51, 63, 286, 288, 371, 413
AccIII TCCGGA 1 cut(s) 454
AclWI GGATC 3 cut(s) 446, 459, 785
AcsI RAATTY 2 cut(s) 893, 988
AcvI CACGTG 1 cut(s) 462
AcyI GRCGYC 2 cut(s) 111, 150
AfaI GTAC 3 cut(s) 596, 600, 887
AfiI CCNNNNNNNGG 4 cut(s) 141, 388, 442, 617
AflIII ACRYGT 2 cut(s) 459, 492
AgsI TTSAA 2 cut(s) 569, 899
AjuI GAANNNNNNNTTGG 2 cut(s) 89, 121
AloI GAACNNNNNNTCC 2 cut(s) 369, 401
AluBI AGCT 7 cut(s) 105, 238, 322, 334, 550, 682, 839
AluI AGCT 7 cut(s) 105, 238, 322, 334, 550, 682, 839
Alw21I GWGCWC 2 cut(s) 684, 841
AlwI GGATC 3 cut(s) 446, 459, 785
AlwNI CAGNNNCTG 1 cut(s) 647
Ama87I CYCGRG 1 cut(s) 551
Aor13HI TCCGGA 1 cut(s) 454
AoxI GGCC 4 cut(s) 293, 555, 575, 652
ApaI GGGCCC 1 cut(s) 656
ApeKI GCWGC 4 cut(s) 200, 392, 907, 999
ApoI RAATTY 2 cut(s) 893, 988
ArsI GACNNNNNNTTYG 4 cut(s) 140, 172, 392, 424
Asp700I GAANNNNTTC 1 cut(s) 666
AspLEI GCGC 3 cut(s) 288, 290, 373
AspS9I GGNCC 6 cut(s) 294, 555, 575, 652, 653, 755
AsuC2I CCSGG 1 cut(s) 389
AsuHPI GGTGA 4 cut(s) 518, 598, 725, 913
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 1 cut(s) 755
BaeGI GKGCMC 1 cut(s) 656
BamHI GGATCC 1 cut(s) 451
BanI GGYRCC 1 cut(s) 607
BanII GRGCYC 3 cut(s) 656, 684, 841
BbrPI CACGTG 1 cut(s) 462
Bbv12I GWGCWC 2 cut(s) 684, 841
BbvCI CCTCAGC 2 cut(s) 447, 648
BbvI GCAGC 4 cut(s) 187, 379, 919, 986
BccI CCATC 4 cut(s) 26, 89, 251, 932
BceAI ACGGC 4 cut(s) 91, 145, 277, 421
BcnI CCSGG 1 cut(s) 389
BfaI CTAG 3 cut(s) 636, 773, 800
BfmI CTRYAG 1 cut(s) 67
BfuAI ACCTGC 1 cut(s) 952
BisI GCNGC 8 cut(s) 49, 201, 265, 268, 393, 414, 908, 1000
BlsI GCNGC 8 cut(s) 50, 202, 266, 269, 394, 415, 909, 1001
Bme1390I CCNGG 1 cut(s) 389
Bme18I GGWCC 1 cut(s) 755
BmeT110I CYCGRG 1 cut(s) 551
BmgT120I GGNCC 6 cut(s) 294, 555, 575, 652, 653, 755
BmiI GGNNCC 9 cut(s) 79, 133, 428, 453, 556, 576, 609, 654, 655
BmrFI CCNGG 1 cut(s) 389
BmrI ACTGGG 2 cut(s) 110, 927
BmsI GCATC 1 cut(s) 285
BmuI ACTGGG 2 cut(s) 110, 927
Bpu10I CCTNAGC 2 cut(s) 447, 648
BpuEI CTTGAG 1 cut(s) 825
BpuMI CCSGG 1 cut(s) 389
BsaAI YACGTR 3 cut(s) 462, 598, 889
BsaBI GATNNNNATC 1 cut(s) 962
BsaHI GRCGYC 2 cut(s) 111, 150
BsaJI CCNNGG 3 cut(s) 49, 259, 441
BsaWI WCCGGW 1 cut(s) 454
BsaXI ACNNNNNCTCC 6 cut(s) 116, 146, 165, 195, 369, 399
Bsc4I CCNNNNNNNGG 4 cut(s) 141, 388, 442, 617
Bse118I RCCGGY 2 cut(s) 557, 577
Bse1I ACTGG 3 cut(s) 116, 146, 933
Bse3DI GCAATG 1 cut(s) 978
Bse8I GATNNNNATC 1 cut(s) 962
BseAI TCCGGA 1 cut(s) 454
BseDI CCNNGG 3 cut(s) 49, 259, 441
BseGI GGATG 2 cut(s) 255, 924
BseJI GATNNNNATC 1 cut(s) 962
BseLI CCNNNNNNNGG 4 cut(s) 141, 388, 442, 617
BseMI GCAATG 1 cut(s) 978
BseMII CTCAG 3 cut(s) 438, 462, 639
BseNI ACTGG 3 cut(s) 116, 146, 933
BsePI GCGCGC 1 cut(s) 286
BseSI GKGCMC 1 cut(s) 656
BseXI GCAGC 4 cut(s) 187, 379, 919, 986
BseYI CCCAGC 1 cut(s) 330
Bsh1236I CGCG 6 cut(s) 51, 63, 286, 288, 371, 413
BshFI GGCC 4 cut(s) 295, 557, 577, 654
BshNI GGYRCC 1 cut(s) 607
BsiHKAI GWGCWC 2 cut(s) 684, 841
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 4 cut(s) 389, 455, 558, 578
BslFI GGGAC 2 cut(s) 98, 637
BslI CCNNNNNNNGG 4 cut(s) 141, 388, 442, 617
BsmFI GGGAC 2 cut(s) 98, 637
BsnI GGCC 4 cut(s) 295, 557, 577, 654
BsoBI CYCGRG 1 cut(s) 551
Bsp120I GGGCCC 1 cut(s) 652
Bsp1286I GDGCHC 3 cut(s) 656, 684, 841
Bsp13I TCCGGA 1 cut(s) 454
Bsp143I GATC 5 cut(s) 451, 730, 790, 820, 832
BspANI GGCC 4 cut(s) 295, 557, 577, 654
BspCNI CTCAG 3 cut(s) 439, 463, 640
BspEI TCCGGA 1 cut(s) 454
BspFNI CGCG 6 cut(s) 51, 63, 286, 288, 371, 413
BspLI GGNNCC 9 cut(s) 79, 133, 428, 453, 556, 576, 609, 654, 655
BspMI ACCTGC 1 cut(s) 952
BspPI GGATC 3 cut(s) 446, 459, 785
BspT107I GGYRCC 1 cut(s) 607
BsrBI CCGCTC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 978
BsrFI RCCGGY 2 cut(s) 557, 577
BsrI ACTGG 3 cut(s) 116, 146, 933
BssAI RCCGGY 2 cut(s) 557, 577
BssECI CCNNGG 3 cut(s) 49, 259, 441
BssHII GCGCGC 1 cut(s) 286
BssMI GATC 5 cut(s) 451, 730, 790, 820, 832
BssNI GRCGYC 2 cut(s) 111, 150
Bst4CI ACNGT 3 cut(s) 314, 612, 628
Bst6I CTCTTC 1 cut(s) 36
BstACI GRCGYC 2 cut(s) 111, 150
BstBAI YACGTR 3 cut(s) 462, 598, 889
BstC8I GCNNGC 5 cut(s) 198, 284, 288, 324, 517
BstDEI CTNAG 3 cut(s) 447, 471, 648
BstDSI CCRYGG 2 cut(s) 49, 259
BstF5I GGATG 2 cut(s) 255, 924
BstFNI CGCG 6 cut(s) 51, 63, 286, 288, 371, 413
BstHHI GCGC 3 cut(s) 288, 290, 373
BstKTI GATC 5 cut(s) 454, 733, 793, 823, 835
BstMBI GATC 5 cut(s) 451, 730, 790, 820, 832
BstMWI GCNNNNNNNGC 3 cut(s) 69, 273, 292
BstSCI CCNGG 1 cut(s) 387
BstSFI CTRYAG 1 cut(s) 67
BstSLI GKGCMC 1 cut(s) 656
BstSNI TACGTA 1 cut(s) 598
BstUI CGCG 6 cut(s) 51, 63, 286, 288, 371, 413
BstV1I GCAGC 4 cut(s) 187, 379, 919, 986
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
BsuRI GGCC 4 cut(s) 295, 557, 577, 654
BtgI CCRYGG 2 cut(s) 49, 259
BtsCI GGATG 2 cut(s) 255, 924
BveI ACCTGC 1 cut(s) 952
Cac8I GCNNGC 5 cut(s) 198, 284, 288, 324, 517
CaiI CAGNNNCTG 1 cut(s) 647
CfoI GCGC 3 cut(s) 288, 290, 373
Cfr10I RCCGGY 2 cut(s) 557, 577
Cfr13I GGNCC 6 cut(s) 294, 555, 575, 652, 653, 755
Cfr42I CCGCGG 1 cut(s) 52
CseI GACGC 2 cut(s) 52, 331
Csp6I GTAC 3 cut(s) 595, 599, 886
CspCI CAANNNNNGTGG 2 cut(s) 783, 818
CviAII CATG 1 cut(s) 911
CviQI GTAC 3 cut(s) 595, 599, 886
DdeI CTNAG 3 cut(s) 447, 471, 648
DpnI GATC 5 cut(s) 453, 732, 792, 822, 834
DpnII GATC 5 cut(s) 451, 730, 790, 820, 832
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
EciI GGCGGA 2 cut(s) 173, 494
Ecl136II GAGCTC 2 cut(s) 682, 839
Eco105I TACGTA 1 cut(s) 598
Eco24I GRGCYC 3 cut(s) 656, 684, 841
Eco47I GGWCC 1 cut(s) 755
Eco53kI GAGCTC 2 cut(s) 682, 839
Eco72I CACGTG 1 cut(s) 462
Eco88I CYCGRG 1 cut(s) 551
EcoICRI GAGCTC 2 cut(s) 682, 839
EcoO109I RGGNCCY 2 cut(s) 652, 653
EcoT38I GRGCYC 3 cut(s) 656, 684, 841
FaeI CATG 1 cut(s) 914
FaiI YATR 4 cut(s) 419, 534, 849, 912
FaqI GGGAC 2 cut(s) 98, 637
FatI CATG 1 cut(s) 910
FauI CCCGC 1 cut(s) 512
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 8 cut(s) 49, 201, 265, 268, 393, 414, 908, 1000
FokI GGATG 2 cut(s) 242, 911
FriOI GRGCYC 3 cut(s) 656, 684, 841
Fsp4HI GCNGC 8 cut(s) 49, 201, 265, 268, 393, 414, 908, 1000
FspBI CTAG 3 cut(s) 636, 773, 800
GlaI GCGC 3 cut(s) 287, 289, 372
GluI GCNGC 8 cut(s) 49, 201, 265, 268, 393, 414, 908, 1000
GsaI CCCAGC 1 cut(s) 334
HaeIII GGCC 4 cut(s) 295, 557, 577, 654
HapII CCGG 4 cut(s) 389, 455, 558, 578
HgaI GACGC 2 cut(s) 52, 331
HhaI GCGC 3 cut(s) 288, 290, 373
Hin1I GRCGYC 2 cut(s) 111, 150
Hin1II CATG 1 cut(s) 914
Hin6I GCGC 3 cut(s) 286, 288, 371
HinP1I GCGC 3 cut(s) 286, 288, 371
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HindIII AAGCTT 1 cut(s) 103
HinfI GANTC 2 cut(s) 509, 975
HpaII CCGG 4 cut(s) 389, 455, 558, 578
HphI GGTGA 4 cut(s) 518, 598, 725, 913
Hpy166II GTNNAC 4 cut(s) 67, 121, 139, 745
Hpy188I TCNGA 1 cut(s) 643
Hpy188III TCNNGA 2 cut(s) 455, 842
Hpy8I GTNNAC 4 cut(s) 67, 121, 139, 745
Hpy99I CGWCG 9 cut(s) 77, 113, 128, 131, 155, 226, 347, 350, 407
HpyAV CCTTC 1 cut(s) 893
HpyCH4III ACNGT 3 cut(s) 314, 612, 628
HpyCH4IV ACGT 6 cut(s) 111, 150, 461, 492, 597, 888
HpyCH4V TGCA 5 cut(s) 203, 276, 353, 851, 947
HpyF10VI GCNNNNNNNGC 3 cut(s) 69, 273, 292
HpyF3I CTNAG 3 cut(s) 447, 471, 648
HpySE526I ACGT 6 cut(s) 111, 150, 461, 492, 597, 888
Hsp92I GRCGYC 2 cut(s) 111, 150
Hsp92II CATG 1 cut(s) 914
HspAI GCGC 3 cut(s) 286, 288, 371
Kpn2I TCCGGA 1 cut(s) 454
KspI CCGCGG 1 cut(s) 52
Kzo9I GATC 5 cut(s) 451, 730, 790, 820, 832
LmnI GCTCC 6 cut(s) 83, 137, 243, 339, 432, 679
Lsp1109I GCAGC 4 cut(s) 187, 379, 919, 986
LweI GCATC 1 cut(s) 285
MaeI CTAG 3 cut(s) 636, 773, 800
MaeII ACGT 6 cut(s) 111, 150, 461, 492, 597, 888
MaeIII GTNAC 1 cut(s) 506
MalI GATC 5 cut(s) 453, 732, 792, 822, 834
MbiI CCGCTC 1 cut(s) 90
MboI GATC 5 cut(s) 451, 730, 790, 820, 832
MboII GAAGA 5 cut(s) 53, 238, 788, 815, 910
MfeI CAATTG 2 cut(s) 673, 714
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 3 cut(s) 656, 684, 841
MluCI AATT 7 cut(s) 569, 673, 714, 865, 893, 953, 988
MlyI GAGTC 1 cut(s) 503
MmeI TCCRAC 3 cut(s) 50, 202, 952
MroI TCCGGA 1 cut(s) 454
MroXI GAANNNNTTC 1 cut(s) 666
MseI TTAA 1 cut(s) 962
MspA1I CMGCKG 1 cut(s) 51
MspI CCGG 4 cut(s) 389, 455, 558, 578
MspR9I CCNGG 1 cut(s) 389
MunI CAATTG 2 cut(s) 673, 714
MvnI CGCG 6 cut(s) 51, 63, 286, 288, 371, 413
MwoI GCNNNNNNNGC 3 cut(s) 69, 273, 292
NciI CCSGG 1 cut(s) 389
NdeII GATC 5 cut(s) 451, 730, 790, 820, 832
NlaIII CATG 1 cut(s) 914
NlaIV GGNNCC 9 cut(s) 79, 133, 428, 453, 556, 576, 609, 654, 655
NmeAIII GCCGAG 2 cut(s) 466, 491
NmuCI GTSAC 1 cut(s) 506
PauI GCGCGC 1 cut(s) 286
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 132, 156
PdmI GAANNNNTTC 1 cut(s) 666
PfeI GAWTC 1 cut(s) 975
PkrI GCNGC 8 cut(s) 50, 202, 266, 269, 394, 415, 909, 1001
PleI GAGTC 1 cut(s) 503
PmaCI CACGTG 1 cut(s) 462
PmlI CACGTG 1 cut(s) 462
PpsI GAGTC 1 cut(s) 503
Ppu21I YACGTR 3 cut(s) 462, 598, 889
Psp124BI GAGCTC 2 cut(s) 684, 841
PspCI CACGTG 1 cut(s) 462
PspFI CCCAGC 1 cut(s) 330
PspN4I GGNNCC 9 cut(s) 79, 133, 428, 453, 556, 576, 609, 654, 655
PspOMI GGGCCC 1 cut(s) 652
PspPI GGNCC 6 cut(s) 294, 555, 575, 652, 653, 755
PstNI CAGNNNCTG 1 cut(s) 647
PsuI RGATCY 1 cut(s) 451
PteI GCGCGC 1 cut(s) 286
RsaI GTAC 3 cut(s) 596, 600, 887
RsaNI GTAC 3 cut(s) 595, 599, 886
SacI GAGCTC 2 cut(s) 684, 841
SacII CCGCGG 1 cut(s) 52
SaqAI TTAA 1 cut(s) 962
SatI GCNGC 8 cut(s) 49, 201, 265, 268, 393, 414, 908, 1000
Sau3AI GATC 5 cut(s) 451, 730, 790, 820, 832
Sau96I GGNCC 6 cut(s) 294, 555, 575, 652, 653, 755
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 1 cut(s) 389
SduI GDGCHC 3 cut(s) 656, 684, 841
SfaNI GCATC 1 cut(s) 285
SfcI CTRYAG 1 cut(s) 67
Sfr303I CCGCGG 1 cut(s) 52
SgrBI CCGCGG 1 cut(s) 52
SinI GGWCC 1 cut(s) 755
SmlI CTYRAG 1 cut(s) 840
SmoI CTYRAG 1 cut(s) 840
SnaBI TACGTA 1 cut(s) 598
Sse9I AATT 7 cut(s) 569, 673, 714, 865, 893, 953, 988
SspI AATATT 1 cut(s) 526
SspMI CTAG 3 cut(s) 636, 773, 800
SstI GAGCTC 2 cut(s) 684, 841
StyD4I CCNGG 1 cut(s) 387
TaaI ACNGT 3 cut(s) 314, 612, 628
TaiI ACGT 6 cut(s) 114, 153, 464, 495, 600, 891
TaqI TCGA 3 cut(s) 108, 159, 835
TasI AATT 7 cut(s) 569, 673, 714, 865, 893, 953, 988
TauI GCSGC 4 cut(s) 51, 267, 270, 416
TfiI GAWTC 1 cut(s) 975
Tru1I TTAA 1 cut(s) 962
Tru9I TTAA 1 cut(s) 962
TseFI GTSAC 1 cut(s) 506
TseI GCWGC 4 cut(s) 200, 392, 907, 999
Tsp45I GTSAC 1 cut(s) 506
TspDTI ATGAA 2 cut(s) 434, 967
TspGWI ACGGA 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 755
XapI RAATTY 2 cut(s) 893, 988
XmiI GTMKAC 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 666
XspI CTAG 3 cut(s) 636, 773, 800
ZraI GACGTC 2 cut(s) 112, 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.