MD11G1176900.v1.1

carboxylesterase 15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001599
Physical Location & Seq
Reverse (-)
20982785 .. 20984891
2107 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1176900.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGTCCACACAAAAACTTAAAGACAAGTCACTAAAACTTGCTTTGCCTAATCACCATTGGACCCCACTTCTCCATTTTTTAACCATACGAAAACACCAAAAACGACAGGCAAAACCACCAACTCCATTACTAACCTCCCTATTTCTTTCCCACTTCACTTCCTTTCCCTTCTCACAATTCTCCCATTTATTCCTCACAATCCCTCCAAACATGTCACAAAACACCGCCATCACTCCAACCTTGCCCTACGAAGAATGCCGCGGGGTTCTCCGCGTCTACAGCGACGGGTCCATCGTCCGCTCTCCCAAACCAAGCTTCGACGTCCCTGTCCACGACGACGGCTCCGTTGACTGGAAAGACGTCGTTTTCGACCCAACCAATCAACTCCAACTCCGCCTCTACAAGCCAGCTGCAACGACACACTCCCCCTCGTCGTTGTCCAAAAAGCTCCCCATCTTCTACTACATCCACGGCGGCGGCTTCTGCATCGGCTCTCGCGCTTGGCCCAATTGCCAGAACTACTGTTTTCAGCTCGCCTCCCAGCTCCAATGCGTCGTCGTTGCACCTGACTATCGCCTCGCGCCAGAACACCGCCTCCCGGCTGCCATGGACGACGGCTTCGCGGCTATGAAATGGCTCCAAGCCATAGCCGAGGCTGAGGATCCGGACACGTGGCTGACTGAGGTGGCGGATTTTGGGAACGTGTTTGTTTCGGGTGACTCGGCTGGCGGGAATATTGCTCATAACTTGGCGGTTCAGCTCGGGGCCGGTTCGGTTGAATTGGGGCCGGTTCGGGTGAGAGGGTATGTACTTTTGGCACCGTTTTTTGGTGGGACGGTTTTGGCTAGGTCAGAAGCTGAGGACCCCAAAGAAGCCTTTCTCAATTGGGAGCTCATTGACAGGTTTTGGAGGCTGTCAATACCAATTGGAGAGGACAGAGATCACCCACTTGTGAACCCATTTGGACCAAATAGCCAGAGCCTAGAAGAAGTGGCTTTTGATCCAATCCTAGTGGTTGTAGGTGGAAGTGATCTTCTCAAAGATCGAGCTAAAGATTATGCAAATAGGCTCAAAAATTGGGGAAACAAGGTTGAGTACGTGGAATTTGAAGGACAGCAGCATGGTTTCTTCACCATCCAACCCAGTTCCCAACCTGCAAAGGAATTGATGTTAATCATCAAACGATTCATTGCTCAAAATTCTACTTGCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

405

Amino Acids

45.15

Weight (kDa)

6.5

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 129 - 195 6.1e-07 Carboxylesterase family
BD-FAE PF20434 142 - 252 2.6e-10 BD-FAE
Abhydrolase_3 PF07859 154 - 377 6.2e-62 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Tree File
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 326
AatII GACGTC 2 cut(s) 324, 363
Acc36I ACCTGC 1 cut(s) 1162
AccB1I GGYRCC 1 cut(s) 817
AccBSI CCGCTC 1 cut(s) 300
AccI GTMKAC 1 cut(s) 276
AccII CGCG 5 cut(s) 261, 273, 498, 581, 623
AccIII TCCGGA 1 cut(s) 664
AclWI GGATC 3 cut(s) 656, 669, 995
AcsI RAATTY 2 cut(s) 1103, 1198
AcvI CACGTG 1 cut(s) 672
AcyI GRCGYC 2 cut(s) 321, 360
AfaI GTAC 2 cut(s) 810, 1097
AfiI CCNNNNNNNGG 3 cut(s) 351, 598, 827
AflIII ACRYGT 3 cut(s) 210, 669, 702
AgsI TTSAA 2 cut(s) 779, 1109
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
AloI GAACNNNNNNTCC 2 cut(s) 579, 611
AluBI AGCT 9 cut(s) 315, 410, 448, 532, 544, 760, 857, 892, 1049
AluI AGCT 9 cut(s) 315, 410, 448, 532, 544, 760, 857, 892, 1049
Alw21I GWGCWC 1 cut(s) 894
AlwI GGATC 3 cut(s) 656, 669, 995
AlwNI CAGNNNCTG 1 cut(s) 857
Ama87I CYCGRG 1 cut(s) 761
Aor13HI TCCGGA 1 cut(s) 664
AoxI GGCC 3 cut(s) 503, 765, 785
ApeKI GCWGC 4 cut(s) 410, 602, 1117, 1209
ApoI RAATTY 2 cut(s) 1103, 1198
ArsI GACNNNNNNTTYG 4 cut(s) 350, 382, 602, 634
Asp700I GAANNNNTTC 1 cut(s) 876
AspLEI GCGC 2 cut(s) 500, 583
AspS9I GGNCC 7 cut(s) 60, 288, 504, 765, 785, 862, 965
AsuC2I CCSGG 1 cut(s) 599
AsuHPI GGTGA 5 cut(s) 44, 728, 808, 935, 1123
AvaI CYCGRG 1 cut(s) 761
AvaII GGWCC 4 cut(s) 60, 288, 862, 965
BamHI GGATCC 1 cut(s) 661
BanI GGYRCC 1 cut(s) 817
BanII GRGCYC 1 cut(s) 894
BbrPI CACGTG 1 cut(s) 672
Bbv12I GWGCWC 1 cut(s) 894
BbvCI CCTCAGC 2 cut(s) 657, 858
BbvI GCAGC 4 cut(s) 397, 589, 1129, 1196
BccI CCATC 4 cut(s) 236, 299, 461, 1142
BceAI ACGGC 3 cut(s) 355, 487, 631
BcnI CCSGG 1 cut(s) 599
BfaI CTAG 3 cut(s) 846, 983, 1010
BfmI CTRYAG 1 cut(s) 277
BfuAI ACCTGC 1 cut(s) 1162
BisI GCNGC 8 cut(s) 259, 411, 475, 478, 603, 624, 1118, 1210
BlsI GCNGC 8 cut(s) 260, 412, 476, 479, 604, 625, 1119, 1211
Bme1390I CCNGG 1 cut(s) 599
Bme18I GGWCC 4 cut(s) 60, 288, 862, 965
BmeT110I CYCGRG 1 cut(s) 761
BmgT120I GGNCC 7 cut(s) 60, 288, 504, 765, 785, 862, 965
BmiI GGNNCC 9 cut(s) 62, 289, 343, 638, 663, 766, 786, 819, 864
BmrFI CCNGG 1 cut(s) 599
BmrI ACTGGG 1 cut(s) 1137
BmsI GCATC 1 cut(s) 495
BmuI ACTGGG 1 cut(s) 1137
Bpu10I CCTNAGC 2 cut(s) 657, 858
BpuMI CCSGG 1 cut(s) 599
BsaAI YACGTR 2 cut(s) 672, 1099
BsaBI GATNNNNATC 1 cut(s) 1172
BsaHI GRCGYC 2 cut(s) 321, 360
BsaJI CCNNGG 4 cut(s) 259, 469, 606, 651
BsaWI WCCGGW 1 cut(s) 664
BsaXI ACNNNNNCTCC 8 cut(s) 187, 217, 326, 356, 375, 405, 579, 609
Bsc4I CCNNNNNNNGG 3 cut(s) 351, 598, 827
Bse118I RCCGGY 2 cut(s) 767, 787
Bse1I ACTGG 2 cut(s) 356, 1143
Bse3DI GCAATG 1 cut(s) 1188
Bse8I GATNNNNATC 1 cut(s) 1172
BseAI TCCGGA 1 cut(s) 664
BseDI CCNNGG 4 cut(s) 259, 469, 606, 651
BseGI GGATG 2 cut(s) 465, 1134
BseJI GATNNNNATC 1 cut(s) 1172
BseLI CCNNNNNNNGG 3 cut(s) 351, 598, 827
BseMI GCAATG 1 cut(s) 1188
BseMII CTCAG 3 cut(s) 648, 672, 849
BseNI ACTGG 2 cut(s) 356, 1143
BseXI GCAGC 4 cut(s) 397, 589, 1129, 1196
BseYI CCCAGC 1 cut(s) 540
Bsh1236I CGCG 5 cut(s) 261, 273, 498, 581, 623
BshFI GGCC 3 cut(s) 505, 767, 787
BshNI GGYRCC 1 cut(s) 817
BsiHKAI GWGCWC 1 cut(s) 894
BsiHKCI CYCGRG 1 cut(s) 761
BsiSI CCGG 4 cut(s) 599, 665, 768, 788
BslFI GGGAC 2 cut(s) 308, 847
BslI CCNNNNNNNGG 3 cut(s) 351, 598, 827
BsmFI GGGAC 2 cut(s) 308, 847
BsmI GAATGC 1 cut(s) 260
BsnI GGCC 3 cut(s) 505, 767, 787
BsoBI CYCGRG 1 cut(s) 761
Bsp1286I GDGCHC 1 cut(s) 894
Bsp13I TCCGGA 1 cut(s) 664
Bsp143I GATC 5 cut(s) 661, 940, 1000, 1030, 1042
Bsp19I CCATGG 1 cut(s) 606
BspANI GGCC 3 cut(s) 505, 767, 787
BspCNI CTCAG 3 cut(s) 649, 673, 850
BspEI TCCGGA 1 cut(s) 664
BspFNI CGCG 5 cut(s) 261, 273, 498, 581, 623
BspLI GGNNCC 9 cut(s) 62, 289, 343, 638, 663, 766, 786, 819, 864
BspMI ACCTGC 1 cut(s) 1162
BspPI GGATC 3 cut(s) 656, 669, 995
BspT107I GGYRCC 1 cut(s) 817
BsrBI CCGCTC 1 cut(s) 300
BsrDI GCAATG 1 cut(s) 1188
BsrFI RCCGGY 2 cut(s) 767, 787
BsrI ACTGG 2 cut(s) 356, 1143
BssAI RCCGGY 2 cut(s) 767, 787
BssECI CCNNGG 4 cut(s) 259, 469, 606, 651
BssMI GATC 5 cut(s) 661, 940, 1000, 1030, 1042
BssNI GRCGYC 2 cut(s) 321, 360
BssT1I CCWWGG 1 cut(s) 606
Bst4CI ACNGT 3 cut(s) 524, 822, 838
BstACI GRCGYC 2 cut(s) 321, 360
BstBAI YACGTR 2 cut(s) 672, 1099
BstC8I GCNNGC 3 cut(s) 408, 534, 727
BstDEI CTNAG 3 cut(s) 657, 681, 858
BstDSI CCRYGG 3 cut(s) 259, 469, 606
BstF5I GGATG 2 cut(s) 465, 1134
BstFNI CGCG 5 cut(s) 261, 273, 498, 581, 623
BstHHI GCGC 2 cut(s) 500, 583
BstKTI GATC 5 cut(s) 664, 943, 1003, 1033, 1045
BstMBI GATC 5 cut(s) 661, 940, 1000, 1030, 1042
BstMWI GCNNNNNNNGC 2 cut(s) 279, 483
BstNSI RCATGY 1 cut(s) 214
BstSCI CCNGG 1 cut(s) 597
BstSFI CTRYAG 1 cut(s) 277
BstUI CGCG 5 cut(s) 261, 273, 498, 581, 623
BstV1I GCAGC 4 cut(s) 397, 589, 1129, 1196
BstX2I RGATCY 1 cut(s) 661
BstYI RGATCY 1 cut(s) 661
BsuRI GGCC 3 cut(s) 505, 767, 787
BtgI CCRYGG 3 cut(s) 259, 469, 606
BtsCI GGATG 2 cut(s) 465, 1134
BveI ACCTGC 1 cut(s) 1162
Cac8I GCNNGC 3 cut(s) 408, 534, 727
CaiI CAGNNNCTG 1 cut(s) 857
CfoI GCGC 2 cut(s) 500, 583
Cfr10I RCCGGY 2 cut(s) 767, 787
Cfr13I GGNCC 7 cut(s) 60, 288, 504, 765, 785, 862, 965
Cfr42I CCGCGG 1 cut(s) 262
CseI GACGC 2 cut(s) 262, 541
Csp6I GTAC 2 cut(s) 809, 1096
CspCI CAANNNNNGTGG 2 cut(s) 993, 1028
CviAII CATG 3 cut(s) 211, 607, 1121
CviQI GTAC 2 cut(s) 809, 1096
DdeI CTNAG 3 cut(s) 657, 681, 858
DpnI GATC 5 cut(s) 663, 942, 1002, 1032, 1044
DpnII GATC 5 cut(s) 661, 940, 1000, 1030, 1042
DrdI GACNNNNNNGTC 1 cut(s) 326
DseDI GACNNNNNNGTC 1 cut(s) 326
EciI GGCGGA 2 cut(s) 383, 704
Ecl136II GAGCTC 1 cut(s) 892
Eco130I CCWWGG 1 cut(s) 606
Eco24I GRGCYC 1 cut(s) 894
Eco47I GGWCC 4 cut(s) 60, 288, 862, 965
Eco53kI GAGCTC 1 cut(s) 892
Eco72I CACGTG 1 cut(s) 672
Eco88I CYCGRG 1 cut(s) 761
EcoICRI GAGCTC 1 cut(s) 892
EcoO109I RGGNCCY 1 cut(s) 862
EcoT14I CCWWGG 1 cut(s) 606
EcoT38I GRGCYC 1 cut(s) 894
ErhI CCWWGG 1 cut(s) 606
FaeI CATG 3 cut(s) 214, 610, 1124
FaiI YATR 9 cut(s) 86, 212, 608, 629, 647, 744, 807, 1059, 1122
FaqI GGGAC 2 cut(s) 308, 847
FatI CATG 3 cut(s) 210, 606, 1120
FauI CCCGC 2 cut(s) 254, 722
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 8 cut(s) 259, 411, 475, 478, 603, 624, 1118, 1210
FokI GGATG 2 cut(s) 452, 1121
FriOI GRGCYC 1 cut(s) 894
Fsp4HI GCNGC 8 cut(s) 259, 411, 475, 478, 603, 624, 1118, 1210
FspBI CTAG 3 cut(s) 846, 983, 1010
GlaI GCGC 2 cut(s) 499, 582
GluI GCNGC 8 cut(s) 259, 411, 475, 478, 603, 624, 1118, 1210
GsaI CCCAGC 1 cut(s) 544
HaeIII GGCC 3 cut(s) 505, 767, 787
HapII CCGG 4 cut(s) 599, 665, 768, 788
HgaI GACGC 2 cut(s) 262, 541
HhaI GCGC 2 cut(s) 500, 583
Hin1I GRCGYC 2 cut(s) 321, 360
Hin1II CATG 3 cut(s) 214, 610, 1124
Hin6I GCGC 2 cut(s) 498, 581
HinP1I GCGC 2 cut(s) 498, 581
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 313
HinfI GANTC 2 cut(s) 719, 1185
HpaII CCGG 4 cut(s) 599, 665, 768, 788
HphI GGTGA 5 cut(s) 44, 728, 808, 935, 1123
Hpy166II GTNNAC 5 cut(s) 6, 277, 331, 349, 955
Hpy188I TCNGA 1 cut(s) 853
Hpy188III TCNNGA 1 cut(s) 665
Hpy8I GTNNAC 5 cut(s) 6, 277, 331, 349, 955
Hpy99I CGWCG 9 cut(s) 287, 323, 338, 341, 365, 436, 557, 560, 617
HpyAV CCTTC 2 cut(s) 178, 1103
HpyCH4III ACNGT 3 cut(s) 524, 822, 838
HpyCH4IV ACGT 5 cut(s) 321, 360, 671, 702, 1098
HpyCH4V TGCA 5 cut(s) 413, 486, 563, 1061, 1157
HpyF10VI GCNNNNNNNGC 2 cut(s) 279, 483
HpyF3I CTNAG 3 cut(s) 657, 681, 858
HpySE526I ACGT 5 cut(s) 321, 360, 671, 702, 1098
Hsp92I GRCGYC 2 cut(s) 321, 360
Hsp92II CATG 3 cut(s) 214, 610, 1124
HspAI GCGC 2 cut(s) 498, 581
Kpn2I TCCGGA 1 cut(s) 664
KspI CCGCGG 1 cut(s) 262
Kzo9I GATC 5 cut(s) 661, 940, 1000, 1030, 1042
LmnI GCTCC 5 cut(s) 347, 453, 549, 642, 889
Lsp1109I GCAGC 4 cut(s) 397, 589, 1129, 1196
LweI GCATC 1 cut(s) 495
MaeI CTAG 3 cut(s) 846, 983, 1010
MaeII ACGT 5 cut(s) 321, 360, 671, 702, 1098
MaeIII GTNAC 3 cut(s) 27, 213, 716
MalI GATC 5 cut(s) 663, 942, 1002, 1032, 1044
MbiI CCGCTC 1 cut(s) 300
MboI GATC 5 cut(s) 661, 940, 1000, 1030, 1042
MboII GAAGA 5 cut(s) 263, 448, 998, 1025, 1120
MfeI CAATTG 3 cut(s) 508, 883, 924
MflI RGATCY 1 cut(s) 661
MhlI GDGCHC 1 cut(s) 894
MluCI AATT 9 cut(s) 176, 508, 779, 883, 924, 1075, 1103, 1163, 1198
MlyI GAGTC 1 cut(s) 713
MmeI TCCRAC 3 cut(s) 260, 412, 1162
MroI TCCGGA 1 cut(s) 664
MroXI GAANNNNTTC 1 cut(s) 876
MseI TTAA 3 cut(s) 18, 80, 1172
MspA1I CMGCKG 2 cut(s) 261, 410
MspI CCGG 4 cut(s) 599, 665, 768, 788
MspR9I CCNGG 1 cut(s) 599
MunI CAATTG 3 cut(s) 508, 883, 924
Mva1269I GAATGC 1 cut(s) 260
MvnI CGCG 5 cut(s) 261, 273, 498, 581, 623
MwoI GCNNNNNNNGC 2 cut(s) 279, 483
NciI CCSGG 1 cut(s) 599
NcoI CCATGG 1 cut(s) 606
NdeII GATC 5 cut(s) 661, 940, 1000, 1030, 1042
NlaIII CATG 3 cut(s) 214, 610, 1124
NlaIV GGNNCC 9 cut(s) 62, 289, 343, 638, 663, 766, 786, 819, 864
NmeAIII GCCGAG 2 cut(s) 676, 701
NmuCI GTSAC 3 cut(s) 27, 213, 716
NspI RCATGY 1 cut(s) 214
PciI ACATGT 1 cut(s) 210
PcsI WCGNNNNNNNCGW 3 cut(s) 291, 342, 366
PctI GAATGC 1 cut(s) 260
PdmI GAANNNNTTC 1 cut(s) 876
PfeI GAWTC 1 cut(s) 1185
PkrI GCNGC 8 cut(s) 260, 412, 476, 479, 604, 625, 1119, 1211
PleI GAGTC 1 cut(s) 713
PmaCI CACGTG 1 cut(s) 672
PmlI CACGTG 1 cut(s) 672
PpsI GAGTC 1 cut(s) 713
Ppu21I YACGTR 2 cut(s) 672, 1099
PpuMI RGGWCCY 1 cut(s) 862
PscI ACATGT 1 cut(s) 210
Psp124BI GAGCTC 1 cut(s) 894
Psp5II RGGWCCY 1 cut(s) 862
PspCI CACGTG 1 cut(s) 672
PspFI CCCAGC 1 cut(s) 540
PspN4I GGNNCC 9 cut(s) 62, 289, 343, 638, 663, 766, 786, 819, 864
PspPI GGNCC 7 cut(s) 60, 288, 504, 765, 785, 862, 965
PspPPI RGGWCCY 1 cut(s) 862
PstNI CAGNNNCTG 1 cut(s) 857
PsuI RGATCY 1 cut(s) 661
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 2 cut(s) 810, 1097
RsaNI GTAC 2 cut(s) 809, 1096
SacI GAGCTC 1 cut(s) 894
SacII CCGCGG 1 cut(s) 262
SaqAI TTAA 3 cut(s) 18, 80, 1172
SatI GCNGC 8 cut(s) 259, 411, 475, 478, 603, 624, 1118, 1210
Sau3AI GATC 5 cut(s) 661, 940, 1000, 1030, 1042
Sau96I GGNCC 7 cut(s) 60, 288, 504, 765, 785, 862, 965
SchI GAGTC 1 cut(s) 713
ScrFI CCNGG 1 cut(s) 599
SduI GDGCHC 1 cut(s) 894
SfaNI GCATC 1 cut(s) 495
SfcI CTRYAG 1 cut(s) 277
Sfr303I CCGCGG 1 cut(s) 262
SgrBI CCGCGG 1 cut(s) 262
SinI GGWCC 4 cut(s) 60, 288, 862, 965
Sse9I AATT 9 cut(s) 176, 508, 779, 883, 924, 1075, 1103, 1163, 1198
SspI AATATT 1 cut(s) 736
SspMI CTAG 3 cut(s) 846, 983, 1010
SstI GAGCTC 1 cut(s) 894
StyD4I CCNGG 1 cut(s) 597
StyI CCWWGG 1 cut(s) 606
TaaI ACNGT 3 cut(s) 524, 822, 838
TaiI ACGT 5 cut(s) 324, 363, 674, 705, 1101
TaqI TCGA 3 cut(s) 318, 369, 1045
TasI AATT 9 cut(s) 176, 508, 779, 883, 924, 1075, 1103, 1163, 1198
TatI WGTACW 1 cut(s) 808
TauI GCSGC 4 cut(s) 261, 477, 480, 626
TfiI GAWTC 1 cut(s) 1185
Tru1I TTAA 3 cut(s) 18, 80, 1172
Tru9I TTAA 3 cut(s) 18, 80, 1172
TseFI GTSAC 3 cut(s) 27, 213, 716
TseI GCWGC 4 cut(s) 410, 602, 1117, 1209
Tsp45I GTSAC 3 cut(s) 27, 213, 716
TspDTI ATGAA 2 cut(s) 644, 1177
TspGWI ACGGA 1 cut(s) 334
VpaK11BI GGWCC 4 cut(s) 60, 288, 862, 965
XapI RAATTY 2 cut(s) 1103, 1198
XceI RCATGY 1 cut(s) 214
XmiI GTMKAC 1 cut(s) 276
XmnI GAANNNNTTC 1 cut(s) 876
XspI CTAG 3 cut(s) 846, 983, 1010
ZraI GACGTC 2 cut(s) 322, 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.