FvH4_3g33150

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000682
Physical Location & Seq
Forward (+)
28569276 .. 28572540
3265 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g33150.t3

Sequence Viewer

Length: 1344 bp
ATGGGTAAGGAAAAGTTTCACATCAACATTGTGGTCATTGGCCACGTTGACTCTGGAAAGTCGACCACCACTGGTCACTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATTGAGAGATTCGAGAAGGAGGCTGCTGAGATGAACAAAAGGTCATTCAAGTATGCCTGGGTGTTGGACAAGCTCAAGGCTGAGCGTGAGCGTGGTATTACCATTGATATTGCCTTGTGGAAGTTTGAGACCACCAAGTACTACTGCACCGTCATTGATGCTCCTGGACATCGTGATTTCATCAAGAACATGATTACTGGAACCTCTCAGGCTGATTGTGCCGTCCTCATTATTGACTCCACCACTGGAGGTTTTGAGGCTGGTATCTCCAAGGATGGGCAGACCCGTGAGCACGCACTGCTTGCCTTCACTCTCGGTGTCAGGCAGATGATTTGCTGCTGTAACAAGATGGATGCCACCACTCCCAAGTACTCAAAGGCAAGGTACGATGAAATTGTGAAGGAAGTCTCTTCCTACCTGAAGAAGGTTGGATACAACCCAGACAAAATTGCCTTTGTCCCCATCTCTGGATTTGAGGGTGACAACATGATTGAGAGGTCCACCAACCTTGACTGGTACAAGGGTCCTACCCTCCTTGAGGCTCTTGACCAGATCAACGAGCCAAAGAGGCCCTCAGACAAGCCCCTCCGTCTACCACTTCAGGATGTATACAAGATCGGTGGTATTGGAACTGTGCCAGTGGGACGTGTGGAGACTGGTATCATCAAGCCTGGTATGGTTGTGACTTTTGGTCCTACTGGTCTAACCACTGAGGTTAAGTCTGTGGAGATGCACCATGAGGCTCTCCTTGAGGCTCTTCCTGGTGACAATGTTGGGTTCAATGTCAAGAATGTCGCTGTCAAGGATCTCAAGCGTGGTTTTGTTGCATCCAACTCCAAGGATGACCCTGCCAAGGAAGCTGCTAACTTCACCTCACAGGTCATCATCATGAACCACCCTGGTCAGATTGGAAATGGATATGCTCCAGTTCTTGACTGCCACACCTCCCACATTGCTGTTAAGTTTGGTGAGATTTTGACCAAGATCGACAGACGATCTGGTAAGGAAATTGAGAAGGAGCCCAAGTTCTTGAAGAATGGTGATTCTGGTATGGTTAAGATGCTTCCCACCAAGCCCATGGTTGTTGAGACCTTCTCTGAGTACCCACCTCTTGGTCGTTTTGCTGTGAGGGACATGCGTCAGACTGTTGCTGTTGGAGTCATCAAGAATGTTGAGAAGAAGGATCCATCTGGTGCCAAGGTGACCAAGTCTGCAGCCAAGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

448

Amino Acids

49.38

Weight (kDa)

9.15

Isoelectric Point (pI)

32.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 7 - 223 1.7e-57 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 248 - 313 2.3e-16 Elongation factor Tu domain 2
GTP_EFTU_D3 PF03143 322 - 429 1.3e-26 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 327 - 427 7.7e-37 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1307
AccB7I CCANNNNNTGG 1 cut(s) 1226
AccI GTMKAC 3 cut(s) 62, 706, 723
AclWI GGATC 3 cut(s) 927, 1292, 1305
AcoI YGGCCR 1 cut(s) 40
AcuI CTGAAG 2 cut(s) 554, 698
AfaI GTAC 5 cut(s) 254, 485, 500, 632, 1217
AfiI CCNNNNNNNGG 6 cut(s) 390, 538, 581, 652, 967, 1226
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 3 cut(s) 163, 895, 1147
AhdI GACNNNNNGTC 1 cut(s) 804
AjiI CACGTC 1 cut(s) 761
AjnI CCWGG 5 cut(s) 170, 277, 784, 874, 1012
AloI GAACNNNNNNTCC 2 cut(s) 1124, 1156
AluBI AGCT 3 cut(s) 91, 187, 974
AluI AGCT 3 cut(s) 91, 187, 974
Alw21I GWGCWC 1 cut(s) 408
Alw26I GTCTC 4 cut(s) 236, 526, 761, 1196
AlwI GGATC 3 cut(s) 927, 1292, 1305
AoxI GGCC 2 cut(s) 40, 683
ApeKI GCWGC 4 cut(s) 137, 450, 974, 1328
Asp700I GAANNNNTTC 1 cut(s) 15
AspS9I GGNCC 4 cut(s) 612, 638, 684, 806
AsuHPI GGTGA 6 cut(s) 605, 890, 976, 1094, 1166, 1327
AvaII GGWCC 3 cut(s) 612, 638, 806
BaeI ACNNNNGTAYC 2 cut(s) 622, 655
BalI TGGCCA 1 cut(s) 42
BamHI GGATCC 1 cut(s) 1297
BanI GGYRCC 1 cut(s) 1307
BanII GRGCYC 1 cut(s) 1137
BarI GAAGNNNNNNTAC 2 cut(s) 530, 562
Bbv12I GWGCWC 1 cut(s) 408
BbvI GCAGC 4 cut(s) 124, 437, 961, 1340
BccI CCATC 4 cut(s) 383, 457, 584, 1309
BceAI ACGGC 1 cut(s) 320
BciT130I CCWGG 5 cut(s) 172, 279, 786, 876, 1014
BciVI GTATCC 1 cut(s) 539
BcoDI GTCTC 4 cut(s) 236, 526, 761, 1196
BfmI CTRYAG 1 cut(s) 1326
BfuI GTATCC 1 cut(s) 539
BglI GCCNNNNNGGC 1 cut(s) 682
BisI GCNGC 4 cut(s) 138, 451, 975, 1329
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 4 cut(s) 139, 452, 976, 1330
BmcAI AGTACT 2 cut(s) 254, 485
Bme1390I CCNGG 5 cut(s) 172, 279, 786, 876, 1014
Bme18I GGWCC 3 cut(s) 612, 638, 806
BmeRI GACNNNNNGTC 1 cut(s) 804
BmgBI CACGTC 1 cut(s) 761
BmgT120I GGNCC 4 cut(s) 612, 638, 684, 806
BmiI GGNNCC 5 cut(s) 316, 639, 1134, 1299, 1309
BmrFI CCNGG 5 cut(s) 172, 279, 786, 876, 1014
BmsI GCATC 5 cut(s) 262, 457, 834, 950, 1164
BoxI GACNNNNGTC 1 cut(s) 1251
BplI GAGNNNNNCTC 2 cut(s) 1193, 1225
BpmI CTGGAG 2 cut(s) 381, 1023
Bpu1102I GCTNAGC 1 cut(s) 195
BpuEI CTTGAG 4 cut(s) 173, 671, 884, 908
BsaI GGTCTC 2 cut(s) 236, 1196
BsaJI CCNNGG 7 cut(s) 171, 384, 951, 966, 1012, 1191, 1311
BsaXI ACNNNNNCTCC 2 cut(s) 1124, 1154
Bsc4I CCNNNNNNNGG 6 cut(s) 390, 538, 581, 652, 967, 1226
Bse1I ACTGG 8 cut(s) 76, 316, 364, 632, 752, 775, 817, 1040
Bse3DI GCAATG 1 cut(s) 1065
BseBI CCWGG 5 cut(s) 172, 279, 786, 876, 1014
BseDI CCNNGG 7 cut(s) 171, 384, 951, 966, 1012, 1191, 1311
BseGI GGATG 5 cut(s) 394, 472, 724, 941, 961
BseLI CCNNNNNNNGG 6 cut(s) 390, 538, 581, 652, 967, 1226
BseMI GCAATG 1 cut(s) 1065
BseMII CTCAG 6 cut(s) 132, 186, 335, 702, 816, 1203
BseNI ACTGG 8 cut(s) 76, 316, 364, 632, 752, 775, 817, 1040
BseXI GCAGC 4 cut(s) 124, 437, 961, 1340
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 2 cut(s) 42, 685
BshNI GGYRCC 1 cut(s) 1307
BsiHKAI GWGCWC 1 cut(s) 408
BslFI GGGAC 3 cut(s) 557, 771, 1259
BslI CCNNNNNNNGG 6 cut(s) 390, 538, 581, 652, 967, 1226
BsmAI GTCTC 4 cut(s) 236, 526, 761, 1196
BsmFI GGGAC 3 cut(s) 557, 771, 1259
BsnI GGCC 2 cut(s) 42, 685
Bso31I GGTCTC 2 cut(s) 236, 1196
Bsp1286I GDGCHC 2 cut(s) 408, 1137
Bsp143I GATC 7 cut(s) 81, 666, 729, 919, 1098, 1109, 1297
Bsp1720I GCTNAGC 1 cut(s) 195
Bsp19I CCATGG 1 cut(s) 1191
BspANI GGCC 2 cut(s) 42, 685
BspCNI CTCAG 6 cut(s) 133, 187, 334, 701, 817, 1204
BspHI TCATGA 1 cut(s) 1002
BspLI GGNNCC 5 cut(s) 316, 639, 1134, 1299, 1309
BspMAI CTGCAG 1 cut(s) 1330
BspPI GGATC 3 cut(s) 927, 1292, 1305
BspQI GCTCTTC 1 cut(s) 876
BspT107I GGYRCC 1 cut(s) 1307
BspTNI GGTCTC 2 cut(s) 236, 1196
BsrDI GCAATG 1 cut(s) 1065
BsrI ACTGG 8 cut(s) 76, 316, 364, 632, 752, 775, 817, 1040
BssECI CCNNGG 7 cut(s) 171, 384, 951, 966, 1012, 1191, 1311
BssMI GATC 7 cut(s) 81, 666, 729, 919, 1098, 1109, 1297
BssNAI GTATAC 1 cut(s) 724
BssT1I CCWWGG 5 cut(s) 384, 951, 966, 1191, 1311
Bst1107I GTATAC 1 cut(s) 724
Bst2UI CCWGG 5 cut(s) 172, 279, 786, 876, 1014
Bst4CI ACNGT 3 cut(s) 265, 748, 1261
Bst6I CTCTTC 2 cut(s) 529, 876
BstAPI GCANNNNNTGC 2 cut(s) 412, 416
BstC8I GCNNGC 2 cut(s) 408, 417
BstDEI CTNAG 6 cut(s) 141, 195, 321, 688, 825, 1212
BstDSI CCRYGG 1 cut(s) 1191
BstEII GGTNACC 1 cut(s) 1315
BstENI CCTNNNNNAGG 2 cut(s) 536, 650
BstF5I GGATG 5 cut(s) 394, 472, 724, 941, 961
BstKTI GATC 7 cut(s) 84, 669, 732, 922, 1101, 1112, 1300
BstMAI GTCTC 4 cut(s) 236, 526, 761, 1196
BstMBI GATC 7 cut(s) 81, 666, 729, 919, 1098, 1109, 1297
BstMWI GCNNNNNNNGC 5 cut(s) 332, 412, 416, 682, 971
BstNI CCWGG 5 cut(s) 172, 279, 786, 876, 1014
BstNSI RCATGY 1 cut(s) 1252
BstPAI GACNNNNGTC 1 cut(s) 1251
BstPI GGTNACC 1 cut(s) 1315
BstSCI CCNGG 5 cut(s) 170, 277, 784, 874, 1012
BstSFI CTRYAG 1 cut(s) 1326
BstV1I GCAGC 4 cut(s) 124, 437, 961, 1340
BstX2I RGATCY 2 cut(s) 919, 1297
BstXI CCANNNNNNTGG 1 cut(s) 1192
BstYI RGATCY 2 cut(s) 919, 1297
BstZ17I GTATAC 1 cut(s) 724
BsuI GTATCC 1 cut(s) 539
BsuRI GGCC 2 cut(s) 42, 685
BtgI CCRYGG 1 cut(s) 1191
BtrI CACGTC 1 cut(s) 761
BtsCI GGATG 5 cut(s) 394, 472, 724, 941, 961
BtsI GCAGTG 1 cut(s) 410
BtsIMutI CAGTG 5 cut(s) 69, 357, 410, 759, 822
Cac8I GCNNGC 2 cut(s) 408, 417
CciI TCATGA 1 cut(s) 1002
Cfr13I GGNCC 4 cut(s) 612, 638, 684, 806
CseI GACGC 1 cut(s) 1241
Csp6I GTAC 5 cut(s) 253, 484, 499, 631, 1216
CspCI CAANNNNNGTGG 6 cut(s) 76, 111, 604, 639, 715, 750
CviAII CATG 6 cut(s) 304, 601, 851, 1003, 1192, 1249
CviQI GTAC 5 cut(s) 253, 484, 499, 631, 1216
DdeI CTNAG 6 cut(s) 141, 195, 321, 688, 825, 1212
DpnI GATC 7 cut(s) 83, 668, 731, 921, 1100, 1111, 1299
DpnII GATC 7 cut(s) 81, 666, 729, 919, 1098, 1109, 1297
DriI GACNNNNNGTC 1 cut(s) 804
EaeI YGGCCR 1 cut(s) 40
Eam1104I CTCTTC 2 cut(s) 529, 876
Eam1105I GACNNNNNGTC 1 cut(s) 804
EarI CTCTTC 2 cut(s) 529, 876
Eco130I CCWWGG 5 cut(s) 384, 951, 966, 1191, 1311
Eco24I GRGCYC 1 cut(s) 1137
Eco31I GGTCTC 2 cut(s) 236, 1196
Eco47I GGWCC 3 cut(s) 612, 638, 806
Eco57I CTGAAG 2 cut(s) 554, 698
Eco91I GGTNACC 1 cut(s) 1315
EcoNI CCTNNNNNAGG 2 cut(s) 536, 650
EcoO109I RGGNCCY 2 cut(s) 638, 684
EcoO65I GGTNACC 1 cut(s) 1315
EcoRII CCWGG 5 cut(s) 170, 277, 784, 874, 1012
EcoT14I CCWWGG 5 cut(s) 384, 951, 966, 1191, 1311
EcoT38I GRGCYC 1 cut(s) 1137
ErhI CCWWGG 5 cut(s) 384, 951, 966, 1191, 1311
FaeI CATG 6 cut(s) 307, 604, 854, 1006, 1195, 1252
FaqI GGGAC 3 cut(s) 557, 771, 1259
FatI CATG 6 cut(s) 303, 600, 850, 1002, 1191, 1248
FblI GTMKAC 3 cut(s) 62, 706, 723
Fnu4HI GCNGC 4 cut(s) 138, 451, 975, 1329
FokI GGATG 5 cut(s) 401, 479, 731, 928, 968
FriOI GRGCYC 1 cut(s) 1137
Fsp4HI GCNGC 4 cut(s) 138, 451, 975, 1329
GluI GCNGC 4 cut(s) 138, 451, 975, 1329
GsuI CTGGAG 2 cut(s) 381, 1023
HaeIII GGCC 2 cut(s) 42, 685
HgaI GACGC 1 cut(s) 1241
Hin1II CATG 6 cut(s) 307, 604, 854, 1006, 1195, 1252
HincII GTYRAC 2 cut(s) 49, 63
HindII GTYRAC 2 cut(s) 49, 63
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 5 cut(s) 50, 123, 350, 1157, 1272
HphI GGTGA 6 cut(s) 605, 890, 976, 1094, 1166, 1327
Hpy166II GTNNAC 5 cut(s) 49, 63, 615, 707, 724
Hpy188I TCNGA 4 cut(s) 691, 1020, 1213, 1257
Hpy8I GTNNAC 5 cut(s) 49, 63, 615, 707, 724
HpyAV CCTTC 7 cut(s) 124, 430, 508, 532, 1123, 1216, 1288
HpyCH4III ACNGT 3 cut(s) 265, 748, 1261
HpyCH4IV ACGT 2 cut(s) 45, 760
HpyCH4V TGCA 4 cut(s) 261, 847, 941, 1328
HpyF10VI GCNNNNNNNGC 5 cut(s) 332, 412, 416, 682, 971
HpyF3I CTNAG 6 cut(s) 141, 195, 321, 688, 825, 1212
HpySE526I ACGT 2 cut(s) 45, 760
Hsp92II CATG 6 cut(s) 307, 604, 854, 1006, 1195, 1252
Kzo9I GATC 7 cut(s) 81, 666, 729, 919, 1098, 1109, 1297
LguI GCTCTTC 1 cut(s) 876
LmnI GCTCC 3 cut(s) 280, 1042, 1132
Lsp1109I GCAGC 4 cut(s) 124, 437, 961, 1340
LweI GCATC 5 cut(s) 262, 457, 834, 950, 1164
MaeII ACGT 2 cut(s) 45, 760
MaeIII GTNAC 6 cut(s) 74, 455, 593, 796, 878, 1315
MalI GATC 7 cut(s) 83, 668, 731, 921, 1100, 1111, 1299
MboI GATC 7 cut(s) 81, 666, 729, 919, 1098, 1109, 1297
MboII GAAGA 5 cut(s) 516, 547, 863, 1159, 1303
MflI RGATCY 2 cut(s) 919, 1297
MhlI GDGCHC 2 cut(s) 408, 1137
MlsI TGGCCA 1 cut(s) 42
MluCI AATT 3 cut(s) 507, 561, 1122
MluNI TGGCCA 1 cut(s) 42
MlyI GAGTC 3 cut(s) 44, 344, 1281
MmeI TCCRAC 4 cut(s) 159, 523, 969, 1249
Mox20I TGGCCA 1 cut(s) 42
MroXI GAANNNNTTC 1 cut(s) 15
MscI TGGCCA 1 cut(s) 42
MseI TTAA 3 cut(s) 831, 1074, 1170
MslI CAYNNNNRTG 1 cut(s) 1001
Msp20I TGGCCA 1 cut(s) 42
MspR9I CCNGG 5 cut(s) 172, 279, 786, 876, 1014
MvaI CCWGG 5 cut(s) 172, 279, 786, 876, 1014
MwoI GCNNNNNNNGC 5 cut(s) 332, 412, 416, 682, 971
NcoI CCATGG 1 cut(s) 1191
NdeII GATC 7 cut(s) 81, 666, 729, 919, 1098, 1109, 1297
NlaIII CATG 6 cut(s) 307, 604, 854, 1006, 1195, 1252
NlaIV GGNNCC 5 cut(s) 316, 639, 1134, 1299, 1309
NmuCI GTSAC 5 cut(s) 74, 593, 796, 878, 1315
NspI RCATGY 1 cut(s) 1252
PagI TCATGA 1 cut(s) 1002
PciSI GCTCTTC 1 cut(s) 876
PdmI GAANNNNTTC 1 cut(s) 15
PfeI GAWTC 2 cut(s) 123, 1157
PflFI GACNNNGTC 1 cut(s) 1321
PflMI CCANNNNNTGG 1 cut(s) 1226
PfoI TCCNGGA 1 cut(s) 277
PkrI GCNGC 4 cut(s) 139, 452, 976, 1330
PleI GAGTC 3 cut(s) 44, 344, 1280
PpsI GAGTC 3 cut(s) 44, 344, 1280
PpuMI RGGWCCY 1 cut(s) 638
PshAI GACNNNNGTC 1 cut(s) 1251
Psp5II RGGWCCY 1 cut(s) 638
Psp6I CCWGG 5 cut(s) 170, 277, 784, 874, 1012
PspEI GGTNACC 1 cut(s) 1315
PspGI CCWGG 5 cut(s) 170, 277, 784, 874, 1012
PspN4I GGNNCC 5 cut(s) 316, 639, 1134, 1299, 1309
PspPI GGNCC 4 cut(s) 612, 638, 684, 806
PspPPI RGGWCCY 1 cut(s) 638
PstI CTGCAG 1 cut(s) 1330
PsuI RGATCY 2 cut(s) 919, 1297
PsyI GACNNNGTC 1 cut(s) 1321
RsaI GTAC 5 cut(s) 254, 485, 500, 632, 1217
RsaNI GTAC 5 cut(s) 253, 484, 499, 631, 1216
RseI CAYNNNNRTG 1 cut(s) 1001
SalI GTCGAC 1 cut(s) 61
SapI GCTCTTC 1 cut(s) 876
SaqAI TTAA 3 cut(s) 831, 1074, 1170
SatI GCNGC 4 cut(s) 138, 451, 975, 1329
Sau3AI GATC 7 cut(s) 81, 666, 729, 919, 1098, 1109, 1297
Sau96I GGNCC 4 cut(s) 612, 638, 684, 806
ScaI AGTACT 2 cut(s) 254, 485
SchI GAGTC 3 cut(s) 44, 344, 1281
ScrFI CCNGG 5 cut(s) 172, 279, 786, 876, 1014
SduI GDGCHC 2 cut(s) 408, 1137
SfaNI GCATC 5 cut(s) 262, 457, 834, 950, 1164
SfcI CTRYAG 1 cut(s) 1326
SinI GGWCC 3 cut(s) 612, 638, 806
SmiMI CAYNNNNRTG 1 cut(s) 1001
SmlI CTYRAG 4 cut(s) 188, 650, 863, 923
SmoI CTYRAG 4 cut(s) 188, 650, 863, 923
Sse9I AATT 3 cut(s) 507, 561, 1122
StyD4I CCNGG 5 cut(s) 170, 277, 784, 874, 1012
StyI CCWWGG 5 cut(s) 384, 951, 966, 1191, 1311
TaaI ACNGT 3 cut(s) 265, 748, 1261
TaiI ACGT 2 cut(s) 48, 763
TaqI TCGA 3 cut(s) 62, 126, 1101
TasI AATT 3 cut(s) 507, 561, 1122
TatI WGTACW 2 cut(s) 252, 483
TfiI GAWTC 2 cut(s) 123, 1157
Tru1I TTAA 3 cut(s) 831, 1074, 1170
Tru9I TTAA 3 cut(s) 831, 1074, 1170
TscAI CASTG 5 cut(s) 76, 364, 417, 759, 829
TseFI GTSAC 5 cut(s) 74, 593, 796, 878, 1315
TseI GCWGC 4 cut(s) 137, 450, 974, 1328
Tsp45I GTSAC 5 cut(s) 74, 593, 796, 878, 1315
TspDTI ATGAA 4 cut(s) 161, 283, 519, 1019
TspGWI ACGGA 1 cut(s) 692
TspRI CASTG 5 cut(s) 76, 364, 417, 759, 829
Tth111I GACNNNGTC 1 cut(s) 1321
Van91I CCANNNNNTGG 1 cut(s) 1226
VpaK11BI GGWCC 3 cut(s) 612, 638, 806
XagI CCTNNNNNAGG 2 cut(s) 536, 650
XceI RCATGY 1 cut(s) 1252
XcmI CCANNNNNNNNNTGG 2 cut(s) 50, 1189
XmiI GTMKAC 3 cut(s) 62, 706, 723
XmnI GAANNNNTTC 1 cut(s) 15
ZrmI AGTACT 2 cut(s) 254, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.