pycom11g10820

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000682
Physical Location & Seq
Reverse (-)
9195828 .. 9196768
941 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g10820.1

Sequence Viewer

Length: 843 bp
ATGGGTAAGGAAAAGGTTCACATCAACACTGTGGTCATTGGCCATGTCGACTCTGGGAAGTCAACCACCACTGGTCACTTGATCTACAAGCTTGGAGGTATTGACAAGCATGTCATTGAGAGGTTCGAGAAGGAGGCTGCTGAGATGAACAAGAGGTCATTCAAGTATGCTTGGGTGTTGGACAAGCTCAAGGCTGAGCGCGAGCGTGGTATTACCATTGATATTGCCTTGTGGAAGTTTGAGACTACCAGGTACTACTGCACTGTTATTGATGCTCCTGGACATCGTGATTTCATCAAGAACATGATCACTGGTACCTCCCAGGCTGATTGTGCTGTCCTCATTATTGACTCCACCACTGGTGGTTTTGAGGCCGGTATTTCCAAGGATGGTCAGACCCGTGAGCATGCTCTGCTTGCTTTCACTCTTGGTGTCAAGCAGATGATTTGCTGCTGCAACAAGATGGATGCCACCACTCCCAAGTACTCAAAGGCAAGGTACGATGAAATCATGAAGGAAGTCTCATCCTACCTCAAGAAGGTTGGGTACAACCCTGACAAAATTGCCTTTGTCCCCATTTCTGGATTTGAGGGTGACAACATGATTGAGAGGTCCACCAACCTAGACTGGTACAAGGGACCAACCCTTCTTGAGGCCCTTGACCTGATCAATGAGCCCAAGAGGCCCTCAGACGAGCCCCTCCGTCTCCCACTTCAGGACGTGTACAAGATTGGTGGCATTGGAACTGTGCCTGTGGGACGAGTTGAAACTGGTGTTGTGAAGCCTGGTATGGTTGTGACCTTTGGTCCCACTGGACTGACCACTGAAGTTCAAGTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

281

Amino Acids

31.07

Weight (kDa)

7.59

Isoelectric Point (pI)

28.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 6 - 221 1.5e-56 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
Acc65I GGTACC 1 cut(s) 314
AccB1I GGYRCC 1 cut(s) 314
AccI GTMKAC 1 cut(s) 48
AccII CGCG 1 cut(s) 201
AcoI YGGCCR 1 cut(s) 40
AcuI CTGAAG 1 cut(s) 698
AfaI GTAC 7 cut(s) 254, 316, 485, 500, 548, 632, 725
AfiI CCNNNNNNNGG 4 cut(s) 538, 581, 652, 715
AflIII ACRYGT 1 cut(s) 720
AgsI TTSAA 3 cut(s) 163, 767, 833
AhdI GACNNNNNGTC 1 cut(s) 804
AjiI CACGTC 1 cut(s) 721
AjnI CCWGG 4 cut(s) 248, 277, 321, 784
AluBI AGCT 2 cut(s) 91, 187
AluI AGCT 2 cut(s) 91, 187
Alw26I GTCTC 3 cut(s) 236, 526, 710
AoxI GGCC 4 cut(s) 40, 372, 654, 683
ApeKI GCWGC 3 cut(s) 137, 450, 453
Asp700I GAANNNNTTC 1 cut(s) 15
Asp718I GGTACC 1 cut(s) 314
AspLEI GCGC 1 cut(s) 201
AspS9I GGNCC 5 cut(s) 612, 638, 655, 684, 806
AsuHPI GGTGA 1 cut(s) 605
AvaII GGWCC 3 cut(s) 612, 638, 806
BalI TGGCCA 1 cut(s) 42
BanI GGYRCC 1 cut(s) 314
BanII GRGCYC 2 cut(s) 678, 699
BarI GAAGNNNNNNTAC 2 cut(s) 530, 562
BbvI GCAGC 3 cut(s) 124, 437, 440
BccI CCATC 2 cut(s) 383, 457
BciT130I CCWGG 4 cut(s) 250, 279, 323, 786
BclI TGATCA 2 cut(s) 306, 666
BcoDI GTCTC 3 cut(s) 236, 526, 710
BfaI CTAG 1 cut(s) 623
BglI GCCNNNNNGGC 1 cut(s) 682
BisI GCNGC 3 cut(s) 138, 451, 454
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 3 cut(s) 139, 452, 455
BmcAI AGTACT 1 cut(s) 485
Bme1390I CCNGG 4 cut(s) 250, 279, 323, 786
Bme18I GGWCC 3 cut(s) 612, 638, 806
BmeRI GACNNNNNGTC 1 cut(s) 804
BmgBI CACGTC 1 cut(s) 721
BmgT120I GGNCC 5 cut(s) 612, 638, 655, 684, 806
BmiI GGNNCC 3 cut(s) 316, 639, 808
BmrFI CCNGG 4 cut(s) 250, 279, 323, 786
BmsI GCATC 2 cut(s) 262, 457
Bpu1102I GCTNAGC 1 cut(s) 195
BpuEI CTTGAG 3 cut(s) 173, 518, 671
BsaJI CCNNGG 2 cut(s) 321, 384
Bsc4I CCNNNNNNNGG 4 cut(s) 538, 581, 652, 715
Bse118I RCCGGY 1 cut(s) 374
Bse1I ACTGG 6 cut(s) 76, 316, 364, 632, 775, 817
BseBI CCWGG 4 cut(s) 250, 279, 323, 786
BseDI CCNNGG 2 cut(s) 321, 384
BseGI GGATG 3 cut(s) 394, 472, 524
BseLI CCNNNNNNNGG 4 cut(s) 538, 581, 652, 715
BseMII CTCAG 3 cut(s) 132, 186, 702
BseNI ACTGG 6 cut(s) 76, 316, 364, 632, 775, 817
BseXI GCAGC 3 cut(s) 124, 437, 440
BsgI GTGCAG 1 cut(s) 244
Bsh1236I CGCG 1 cut(s) 201
BshFI GGCC 4 cut(s) 42, 374, 656, 685
BshNI GGYRCC 1 cut(s) 314
BsiSI CCGG 1 cut(s) 375
BslFI GGGAC 4 cut(s) 557, 651, 771, 792
BslI CCNNNNNNNGG 4 cut(s) 538, 581, 652, 715
BsmAI GTCTC 3 cut(s) 236, 526, 710
BsmBI CGTCTC 1 cut(s) 710
BsmFI GGGAC 4 cut(s) 557, 651, 771, 792
BsnI GGCC 4 cut(s) 42, 374, 656, 685
Bsp1286I GDGCHC 2 cut(s) 678, 699
Bsp1407I TGTACA 1 cut(s) 723
Bsp143I GATC 3 cut(s) 81, 306, 666
Bsp1720I GCTNAGC 1 cut(s) 195
BspANI GGCC 4 cut(s) 42, 374, 656, 685
BspCNI CTCAG 3 cut(s) 133, 187, 701
BspFNI CGCG 1 cut(s) 201
BspHI TCATGA 1 cut(s) 510
BspLI GGNNCC 3 cut(s) 316, 639, 808
BspT107I GGYRCC 1 cut(s) 314
BsrFI RCCGGY 1 cut(s) 374
BsrGI TGTACA 1 cut(s) 723
BsrI ACTGG 6 cut(s) 76, 316, 364, 632, 775, 817
BssAI RCCGGY 1 cut(s) 374
BssECI CCNNGG 2 cut(s) 321, 384
BssMI GATC 3 cut(s) 81, 306, 666
BssT1I CCWWGG 1 cut(s) 384
Bst2UI CCWGG 4 cut(s) 250, 279, 323, 786
Bst4CI ACNGT 3 cut(s) 31, 265, 748
BstAPI GCANNNNNTGC 1 cut(s) 412
BstAUI TGTACA 1 cut(s) 723
BstC8I GCNNGC 3 cut(s) 203, 408, 417
BstDEI CTNAG 3 cut(s) 141, 195, 688
BstENI CCTNNNNNAGG 2 cut(s) 536, 650
BstF5I GGATG 3 cut(s) 394, 472, 524
BstFNI CGCG 1 cut(s) 201
BstHHI GCGC 1 cut(s) 201
BstKTI GATC 3 cut(s) 84, 309, 669
BstMAI GTCTC 3 cut(s) 236, 526, 710
BstMBI GATC 3 cut(s) 81, 306, 666
BstMWI GCNNNNNNNGC 4 cut(s) 332, 412, 416, 682
BstNI CCWGG 4 cut(s) 250, 279, 323, 786
BstNSI RCATGY 2 cut(s) 113, 410
BstSCI CCNGG 4 cut(s) 248, 277, 321, 784
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 3 cut(s) 124, 437, 440
BsuRI GGCC 4 cut(s) 42, 374, 656, 685
BtrI CACGTC 1 cut(s) 721
BtsCI GGATG 3 cut(s) 394, 472, 524
BtsIMutI CAGTG 7 cut(s) 27, 69, 261, 309, 357, 810, 822
Cac8I GCNNGC 3 cut(s) 203, 408, 417
CciI TCATGA 1 cut(s) 510
CfoI GCGC 1 cut(s) 201
Cfr10I RCCGGY 1 cut(s) 374
Cfr13I GGNCC 5 cut(s) 612, 638, 655, 684, 806
CsiI ACCWGGT 1 cut(s) 248
Csp6I GTAC 7 cut(s) 253, 315, 484, 499, 547, 631, 724
CspCI CAANNNNNGTGG 2 cut(s) 715, 750
CviAII CATG 6 cut(s) 44, 110, 304, 407, 511, 601
CviQI GTAC 7 cut(s) 253, 315, 484, 499, 547, 631, 724
DdeI CTNAG 3 cut(s) 141, 195, 688
DpnI GATC 3 cut(s) 83, 308, 668
DpnII GATC 3 cut(s) 81, 306, 666
DrdI GACNNNNNNGTC 1 cut(s) 110
DriI GACNNNNNGTC 1 cut(s) 804
DseDI GACNNNNNNGTC 1 cut(s) 110
EaeI YGGCCR 1 cut(s) 40
Eam1105I GACNNNNNGTC 1 cut(s) 804
Eco130I CCWWGG 1 cut(s) 384
Eco24I GRGCYC 2 cut(s) 678, 699
Eco47I GGWCC 3 cut(s) 612, 638, 806
Eco57I CTGAAG 1 cut(s) 698
EcoNI CCTNNNNNAGG 2 cut(s) 536, 650
EcoO109I RGGNCCY 2 cut(s) 655, 684
EcoRII CCWGG 4 cut(s) 248, 277, 321, 784
EcoT14I CCWWGG 1 cut(s) 384
EcoT38I GRGCYC 2 cut(s) 678, 699
ErhI CCWWGG 1 cut(s) 384
Esp3I CGTCTC 1 cut(s) 710
FaeI CATG 6 cut(s) 47, 113, 307, 410, 514, 604
FaiI YATR 8 cut(s) 45, 111, 168, 305, 408, 512, 602, 791
FaqI GGGAC 4 cut(s) 557, 651, 771, 792
FatI CATG 6 cut(s) 43, 109, 303, 406, 510, 600
FbaI TGATCA 2 cut(s) 306, 666
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 3 cut(s) 138, 451, 454
FokI GGATG 3 cut(s) 401, 479, 511
FriOI GRGCYC 2 cut(s) 678, 699
Fsp4HI GCNGC 3 cut(s) 138, 451, 454
FspBI CTAG 1 cut(s) 623
GlaI GCGC 1 cut(s) 200
GluI GCNGC 3 cut(s) 138, 451, 454
HaeIII GGCC 4 cut(s) 42, 374, 656, 685
HapII CCGG 1 cut(s) 375
HhaI GCGC 1 cut(s) 201
Hin1II CATG 6 cut(s) 47, 113, 307, 410, 514, 604
Hin6I GCGC 1 cut(s) 199
HinP1I GCGC 1 cut(s) 199
HincII GTYRAC 2 cut(s) 49, 63
HindII GTYRAC 2 cut(s) 49, 63
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 2 cut(s) 50, 350
HpaII CCGG 1 cut(s) 375
HphI GGTGA 1 cut(s) 605
Hpy166II GTNNAC 5 cut(s) 19, 49, 63, 615, 724
Hpy188I TCNGA 2 cut(s) 396, 691
Hpy188III TCNNGA 8 cut(s) 127, 287, 298, 511, 535, 582, 650, 716
Hpy8I GTNNAC 5 cut(s) 19, 49, 63, 615, 724
HpyAV CCTTC 4 cut(s) 124, 508, 532, 656
HpyCH4III ACNGT 3 cut(s) 31, 265, 748
HpyCH4IV ACGT 1 cut(s) 720
HpyCH4V TGCA 2 cut(s) 261, 456
HpyF10VI GCNNNNNNNGC 4 cut(s) 332, 412, 416, 682
HpyF3I CTNAG 3 cut(s) 141, 195, 688
HpySE526I ACGT 1 cut(s) 720
Hsp92II CATG 6 cut(s) 47, 113, 307, 410, 514, 604
HspAI GCGC 1 cut(s) 199
KpnI GGTACC 1 cut(s) 318
Ksp22I TGATCA 2 cut(s) 306, 666
Kzo9I GATC 3 cut(s) 81, 306, 666
LmnI GCTCC 1 cut(s) 280
Lsp1109I GCAGC 3 cut(s) 124, 437, 440
LweI GCATC 2 cut(s) 262, 457
MabI ACCWGGT 1 cut(s) 248
MaeI CTAG 1 cut(s) 623
MaeII ACGT 1 cut(s) 720
MaeIII GTNAC 3 cut(s) 74, 593, 796
MalI GATC 3 cut(s) 83, 308, 668
MboI GATC 3 cut(s) 81, 306, 666
MhlI GDGCHC 2 cut(s) 678, 699
MlsI TGGCCA 1 cut(s) 42
MluCI AATT 1 cut(s) 561
MluNI TGGCCA 1 cut(s) 42
MlyI GAGTC 2 cut(s) 44, 344
MmeI TCCRAC 1 cut(s) 159
Mox20I TGGCCA 1 cut(s) 42
MroXI GAANNNNTTC 1 cut(s) 15
MscI TGGCCA 1 cut(s) 42
Msp20I TGGCCA 1 cut(s) 42
MspI CCGG 1 cut(s) 375
MspR9I CCNGG 4 cut(s) 250, 279, 323, 786
MvaI CCWGG 4 cut(s) 250, 279, 323, 786
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 4 cut(s) 332, 412, 416, 682
NdeII GATC 3 cut(s) 81, 306, 666
NlaIII CATG 6 cut(s) 47, 113, 307, 410, 514, 604
NlaIV GGNNCC 3 cut(s) 316, 639, 808
NmuCI GTSAC 3 cut(s) 74, 593, 796
NspI RCATGY 2 cut(s) 113, 410
PaeI GCATGC 1 cut(s) 410
PagI TCATGA 1 cut(s) 510
PdmI GAANNNNTTC 1 cut(s) 15
PfoI TCCNGGA 1 cut(s) 277
PkrI GCNGC 3 cut(s) 139, 452, 455
PleI GAGTC 2 cut(s) 44, 344
PpsI GAGTC 2 cut(s) 44, 344
Psp6I CCWGG 4 cut(s) 248, 277, 321, 784
PspGI CCWGG 4 cut(s) 248, 277, 321, 784
PspN4I GGNNCC 3 cut(s) 316, 639, 808
PspPI GGNCC 5 cut(s) 612, 638, 655, 684, 806
RsaI GTAC 7 cut(s) 254, 316, 485, 500, 548, 632, 725
RsaNI GTAC 7 cut(s) 253, 315, 484, 499, 547, 631, 724
SalI GTCGAC 1 cut(s) 47
SatI GCNGC 3 cut(s) 138, 451, 454
Sau3AI GATC 3 cut(s) 81, 306, 666
Sau96I GGNCC 5 cut(s) 612, 638, 655, 684, 806
ScaI AGTACT 1 cut(s) 485
SchI GAGTC 2 cut(s) 44, 344
ScrFI CCNGG 4 cut(s) 250, 279, 323, 786
SduI GDGCHC 2 cut(s) 678, 699
SexAI ACCWGGT 1 cut(s) 248
SfaNI GCATC 2 cut(s) 262, 457
SinI GGWCC 3 cut(s) 612, 638, 806
SmlI CTYRAG 3 cut(s) 188, 533, 650
SmoI CTYRAG 3 cut(s) 188, 533, 650
SphI GCATGC 1 cut(s) 410
Sse9I AATT 1 cut(s) 561
SspMI CTAG 1 cut(s) 623
StyD4I CCNGG 4 cut(s) 248, 277, 321, 784
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 3 cut(s) 31, 265, 748
TaiI ACGT 1 cut(s) 723
TaqI TCGA 2 cut(s) 48, 126
TasI AATT 1 cut(s) 561
TatI WGTACW 2 cut(s) 483, 723
TscAI CASTG 7 cut(s) 34, 76, 268, 316, 364, 817, 829
TseFI GTSAC 3 cut(s) 74, 593, 796
TseI GCWGC 3 cut(s) 137, 450, 453
Tsp45I GTSAC 3 cut(s) 74, 593, 796
TspDTI ATGAA 4 cut(s) 161, 283, 519, 527
TspGWI ACGGA 1 cut(s) 692
TspRI CASTG 7 cut(s) 34, 76, 268, 316, 364, 817, 829
VpaK11BI GGWCC 3 cut(s) 612, 638, 806
XagI CCTNNNNNAGG 2 cut(s) 536, 650
XceI RCATGY 2 cut(s) 113, 410
XcmI CCANNNNNNNNNTGG 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 48
XmnI GAANNNNTTC 1 cut(s) 15
XspI CTAG 1 cut(s) 623
ZrmI AGTACT 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.