Rorug05G0329800

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000682
Physical Location & Seq
Forward (+)
39758851 .. 39762845
3995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0329800.1

Sequence Viewer

Length: 831 bp
ATGGTTTTGGTATGTCGATTATGCTCTCCTAACCACACCGGAAAAGCCCAACAAGCCAACCAAAGTTGCTCTTCCGATTTGGCCGTAAATGATGAAAACCAGGCTCCACTTCCGGCAACCATTTACTACATTTCCTCTGAGAATCTCAAAGATTCCAAGATTCAGGACTGGATTCAAAGGATTGGACTCAAGTGTGCGATTCTCTCAACAATGCTAGGCGTTTTGCTCTGCTCTTTTGGTGCCTACTCTCTAAAAAGACTATCCTTATGGACTAAGCAAGTAAGAGTTAGCTCTGGGAATGGGATGCCTCTTGGAGATGGCCATATCTGGATTAAATATGGGCAGAAGGAAATTCTAGGAGCCAAATATCCCAGGTTACATCAAAAACCCAAAAAGACACTTGATGGAAGAAGCCATGACCTGTGCTCAGCAAATGTGCATTTCAGCTTCGGACCTCTGTGTCAATATCTATCTGTAAGCTACTTGGATCGCGGTCTTTCAGCCTATGAAATACCTAATGGCAAAGCTTGGACTATGCAATTGTTGGCTGTGGCATGTTTGCCCCTTGCGGCCAAAATAAAGGAGATTAATGTCCCACTCTACCTTGATTTACAGGTGGCTGGGTTAAAAAATGTATTCGAGGCCATTGAGAAGGAGATGTGCAAGTTTCTAGATCTTCTGTTCAACGATTCATTTTTGGTTGAACAGAAATATGTTATCACAACTGAGGGTCATCCCCTTCCAGTGTTAAGTAGTTGGCAGGACAGGCTTCTAGCATCCAAACTAACTGGACTTCAATCAAGGTCTTGCTTCTACCAAAGTGTATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

276

Amino Acids

30.83

Weight (kDa)

8.83

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclin_N PF00134 145 - 208 2.6e-11 Cyclin, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 459
AccB1I GGYRCC 1 cut(s) 239
AccI GTMKAC 1 cut(s) 825
AccII CGCG 1 cut(s) 492
AciI CCGC 2 cut(s) 492, 569
AclWI GGATC 1 cut(s) 495
AcoI YGGCCR 3 cut(s) 81, 319, 570
AcsI RAATTY 1 cut(s) 351
AgsI TTSAA 4 cut(s) 176, 685, 704, 797
AjnI CCWGG 2 cut(s) 99, 371
AluBI AGCT 4 cut(s) 291, 447, 480, 527
AluI AGCT 4 cut(s) 291, 447, 480, 527
Alw21I GWGCWC 1 cut(s) 428
AlwI GGATC 1 cut(s) 495
AoxI GGCC 4 cut(s) 81, 319, 570, 642
ApoI RAATTY 1 cut(s) 351
AseI ATTAAT 1 cut(s) 588
AspS9I GGNCC 1 cut(s) 452
AvaII GGWCC 1 cut(s) 452
BalI TGGCCA 1 cut(s) 321
BanI GGYRCC 1 cut(s) 239
Bbv12I GWGCWC 1 cut(s) 428
BccI CCATC 2 cut(s) 311, 398
BceAI ACGGC 1 cut(s) 68
BciT130I CCWGG 2 cut(s) 101, 373
BfaI CTAG 4 cut(s) 215, 356, 671, 773
BglII AGATCT 1 cut(s) 673
BisI GCNGC 1 cut(s) 570
BlpI GCTNAGC 1 cut(s) 427
BlsI GCNGC 1 cut(s) 571
Bme1390I CCNGG 2 cut(s) 101, 373
Bme18I GGWCC 1 cut(s) 452
BmgT120I GGNCC 1 cut(s) 452
BmiI GGNNCC 3 cut(s) 105, 241, 361
BmrFI CCNGG 2 cut(s) 101, 373
BmsI GCATC 2 cut(s) 294, 785
Bpu1102I GCTNAGC 1 cut(s) 427
BpuEI CTTGAG 1 cut(s) 173
BsaJI CCNNGG 1 cut(s) 371
BsaWI WCCGGW 1 cut(s) 38
Bse1I ACTGG 3 cut(s) 173, 743, 793
BseBI CCWGG 2 cut(s) 101, 373
BseDI CCNNGG 1 cut(s) 371
BseGI GGATG 3 cut(s) 309, 733, 776
BseMII CTCAG 3 cut(s) 129, 441, 717
BseNI ACTGG 3 cut(s) 173, 743, 793
BseYI CCCAGC 1 cut(s) 620
Bsh1236I CGCG 1 cut(s) 492
BshFI GGCC 4 cut(s) 83, 321, 572, 644
BshNI GGYRCC 1 cut(s) 239
BsiHKAI GWGCWC 1 cut(s) 428
BsiSI CCGG 2 cut(s) 39, 113
BslFI GGGAC 1 cut(s) 578
BsmFI GGGAC 1 cut(s) 578
BsnI GGCC 4 cut(s) 83, 321, 572, 644
Bsp1286I GDGCHC 1 cut(s) 428
Bsp143I GATC 2 cut(s) 487, 673
Bsp1720I GCTNAGC 1 cut(s) 427
BspACI CCGC 2 cut(s) 492, 569
BspANI GGCC 4 cut(s) 83, 321, 572, 644
BspCNI CTCAG 3 cut(s) 130, 440, 718
BspFNI CGCG 1 cut(s) 492
BspLI GGNNCC 3 cut(s) 105, 241, 361
BspPI GGATC 1 cut(s) 495
BspQI GCTCTTC 1 cut(s) 76
BspT107I GGYRCC 1 cut(s) 239
BsrI ACTGG 3 cut(s) 173, 743, 793
BssECI CCNNGG 1 cut(s) 371
BssMI GATC 2 cut(s) 487, 673
BssNAI GTATAC 1 cut(s) 826
Bst1107I GTATAC 1 cut(s) 826
Bst2UI CCWGG 2 cut(s) 101, 373
Bst6I CTCTTC 1 cut(s) 76
BstDEI CTNAG 4 cut(s) 138, 273, 427, 726
BstF5I GGATG 3 cut(s) 309, 733, 776
BstFNI CGCG 1 cut(s) 492
BstKTI GATC 2 cut(s) 490, 676
BstMBI GATC 2 cut(s) 487, 673
BstMWI GCNNNNNNNGC 2 cut(s) 53, 766
BstNI CCWGG 2 cut(s) 101, 373
BstNSI RCATGY 1 cut(s) 558
BstSCI CCNGG 2 cut(s) 99, 371
BstUI CGCG 1 cut(s) 492
BstX2I RGATCY 1 cut(s) 673
BstYI RGATCY 1 cut(s) 673
BstZ17I GTATAC 1 cut(s) 826
BsuRI GGCC 4 cut(s) 83, 321, 572, 644
BtsCI GGATG 3 cut(s) 309, 733, 776
BtsIMutI CAGTG 1 cut(s) 750
Cfr13I GGNCC 1 cut(s) 452
CviAII CATG 2 cut(s) 416, 555
DdeI CTNAG 4 cut(s) 138, 273, 427, 726
DpnI GATC 2 cut(s) 489, 675
DpnII GATC 2 cut(s) 487, 673
DrdI GACNNNNNNGTC 1 cut(s) 459
DseDI GACNNNNNNGTC 1 cut(s) 459
EaeI YGGCCR 3 cut(s) 81, 319, 570
Eam1104I CTCTTC 1 cut(s) 76
EarI CTCTTC 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 452
EcoRII CCWGG 2 cut(s) 99, 371
FaeI CATG 2 cut(s) 419, 558
FalI AAGNNNNNCTT 2 cut(s) 55, 87
FaqI GGGAC 1 cut(s) 578
FatI CATG 2 cut(s) 415, 554
FblI GTMKAC 1 cut(s) 825
Fnu4HI GCNGC 1 cut(s) 570
FokI GGATG 3 cut(s) 316, 720, 763
Fsp4HI GCNGC 1 cut(s) 570
FspBI CTAG 4 cut(s) 215, 356, 671, 773
GluI GCNGC 1 cut(s) 570
GsaI CCCAGC 1 cut(s) 624
HaeIII GGCC 4 cut(s) 83, 321, 572, 644
HapII CCGG 2 cut(s) 39, 113
Hin1II CATG 2 cut(s) 419, 558
HindIII AAGCTT 1 cut(s) 525
HinfI GANTC 7 cut(s) 142, 152, 160, 172, 186, 199, 689
HpaII CCGG 2 cut(s) 39, 113
Hpy166II GTNNAC 1 cut(s) 826
Hpy188I TCNGA 3 cut(s) 76, 139, 452
Hpy188III TCNNGA 3 cut(s) 164, 328, 671
Hpy8I GTNNAC 1 cut(s) 826
HpyAV CCTTC 3 cut(s) 340, 646, 749
HpyCH4V TGCA 3 cut(s) 439, 538, 663
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 766
HpyF3I CTNAG 4 cut(s) 138, 273, 427, 726
Hsp92II CATG 2 cut(s) 419, 558
Kzo9I GATC 2 cut(s) 487, 673
LguI GCTCTTC 1 cut(s) 76
LmnI GCTCC 2 cut(s) 109, 359
LweI GCATC 2 cut(s) 294, 785
MaeI CTAG 4 cut(s) 215, 356, 671, 773
MaeIII GTNAC 1 cut(s) 375
MalI GATC 2 cut(s) 489, 675
MboI GATC 2 cut(s) 487, 673
MboII GAAGA 3 cut(s) 63, 420, 668
MfeI CAATTG 1 cut(s) 539
MflI RGATCY 1 cut(s) 673
MhlI GDGCHC 1 cut(s) 428
MlsI TGGCCA 1 cut(s) 321
MluCI AATT 2 cut(s) 351, 539
MluNI TGGCCA 1 cut(s) 321
MlyI GAGTC 1 cut(s) 180
MnlI CCTC 5 cut(s) 145, 318, 465, 634, 721
Mox20I TGGCCA 1 cut(s) 321
MscI TGGCCA 1 cut(s) 321
MseI TTAA 4 cut(s) 333, 588, 626, 749
Msp20I TGGCCA 1 cut(s) 321
MspI CCGG 2 cut(s) 39, 113
MspR9I CCNGG 2 cut(s) 101, 373
MunI CAATTG 1 cut(s) 539
MvaI CCWGG 2 cut(s) 101, 373
MvnI CGCG 1 cut(s) 492
MwoI GCNNNNNNNGC 2 cut(s) 53, 766
NdeII GATC 2 cut(s) 487, 673
NlaIII CATG 2 cut(s) 419, 558
NlaIV GGNNCC 3 cut(s) 105, 241, 361
NspI RCATGY 1 cut(s) 558
PciSI GCTCTTC 1 cut(s) 76
PfeI GAWTC 6 cut(s) 142, 152, 160, 172, 199, 689
PkrI GCNGC 1 cut(s) 571
PleI GAGTC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 180
PshBI ATTAAT 1 cut(s) 588
Psp6I CCWGG 2 cut(s) 99, 371
PspFI CCCAGC 1 cut(s) 620
PspGI CCWGG 2 cut(s) 99, 371
PspN4I GGNNCC 3 cut(s) 105, 241, 361
PspPI GGNCC 1 cut(s) 452
PsuI RGATCY 1 cut(s) 673
SapI GCTCTTC 1 cut(s) 76
SaqAI TTAA 4 cut(s) 333, 588, 626, 749
SatI GCNGC 1 cut(s) 570
Sau3AI GATC 2 cut(s) 487, 673
Sau96I GGNCC 1 cut(s) 452
SchI GAGTC 1 cut(s) 180
ScrFI CCNGG 2 cut(s) 101, 373
SduI GDGCHC 1 cut(s) 428
SfaNI GCATC 2 cut(s) 294, 785
SinI GGWCC 1 cut(s) 452
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 2 cut(s) 351, 539
SsiI CCGC 2 cut(s) 492, 569
SspMI CTAG 4 cut(s) 215, 356, 671, 773
StyD4I CCNGG 2 cut(s) 99, 371
TaqI TCGA 2 cut(s) 16, 639
TasI AATT 2 cut(s) 351, 539
TauI GCSGC 1 cut(s) 572
TfiI GAWTC 6 cut(s) 142, 152, 160, 172, 199, 689
Tru1I TTAA 4 cut(s) 333, 588, 626, 749
Tru9I TTAA 4 cut(s) 333, 588, 626, 749
TscAI CASTG 1 cut(s) 750
TspDTI ATGAA 3 cut(s) 108, 522, 681
TspRI CASTG 1 cut(s) 750
VpaK11BI GGWCC 1 cut(s) 452
VspI ATTAAT 1 cut(s) 588
XapI RAATTY 1 cut(s) 351
XbaI TCTAGA 1 cut(s) 670
XceI RCATGY 1 cut(s) 558
XmiI GTMKAC 1 cut(s) 825
XspI CTAG 4 cut(s) 215, 356, 671, 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.