MD04G1011300.v1.1

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000682
Physical Location & Seq
Reverse (-)
1278422 .. 1281105
2684 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1011300.v1.1.491

Sequence Viewer

Length: 1200 bp
ATGAACAAAAGGTCATTCAAGTATGCCTGGGTGCTTGACAAGCTTAAGGCCGAGCGTGAGCGTGGTATCACCATTGATATCGCCTTGTGGAAGTTTGAGACCACAAGGTACTACTGCACAGTCATTGATGCTCCGGGACATCGTGATTTCATTAAGAACATGATTACTGGAACATCACAGGCTGACTGTGCTATCCTAATCATTGACTCCACCACTGGTGGTTTCGAAGCTGGTATTTCCAAGGACGGACAGACCCGTGAGCATGCTCTTCTTGCTTTCACTCTAGGTGTGAGGCAGATGATTTGCTGCTGTAACAAGATGGATGCCACCACTCCTAAGTACTCTAAGGCAAGGTACGATGAAATCGTGAAGGAAGTTTCATCCTACCTGAAGAAGGTTGGGTACAACCCCGACAAAATCGCCTTTGTTCCCATCTCTGGGTTTGAGGGTGACAACATGATTGAGAGGTCCACCAACCTTGACTGGTACAAGGGACCAACCCTTCTTGAGGCTCTTGACCAGATCAACGAGCCCAAGAGACCCTCAGACAAGCCCCTCCGTCTTCCTCTTCAGGATGTGTACAAGATTGGTGGTATTGGAACTGTGCCAGTGGGACGTGTAGAGACTGGTATCGTCAAGCCTGGTATGGTTGTGACTTTTGGGCCAACTGGTCTGACCACTGAAGTTAAGTCAGTTGAGATGCATCACGAGGCTCTTCAGGAGGCACTCCCCGGTGACAATGTCGGGTTCAATGTTAAGAATGTTGCTGTGAAGGATCTCAAACGTGGTTTCGTCGCATCCAACTCCAAGGATGACCCTGCCAAGGAGGCTGCCAACTTTACAGCCCAGGTTATCATCATGAACCACCCTGGTCAGATCAGTAACGGATATGCCCCAGTTCTGGATTGCCACACTTCTCACATTGCCGTGCGGTTCGGTGAGATCCTTACCAAGATTGACAGGAGGTCTGGAAAGGAGATTGAGAAGGAGCCAAAGTTTTTGAAGAACGGAGATGCAGGTATGGTGAAGATGCTTCCCACCAAGCCCATGGTTGTGGAGACCTTCTCTGAGTACCCACCATTGGGTCGCTTTGCTGTTCGTGACATGCGCCAGACTGTTGCTGTCGGTGTTATCAAGAGCGTTGAGAAGAAGGACCCCAGCGGTGCCAAGGTGACCAAGTCTGCTGCCAAGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

400

Amino Acids

44.09

Weight (kDa)

9.16

Isoelectric Point (pI)

32.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 8 - 175 1.4e-42 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 200 - 265 2.6e-17 Elongation factor Tu domain 2
GTP_EFTU_D3 PF03143 274 - 381 2.2e-27 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 279 - 379 3.4e-38 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1007
AccB1I GGYRCC 1 cut(s) 1163
AciI CCGC 2 cut(s) 931, 1161
AclWI GGATC 2 cut(s) 783, 937
AcuI CTGAAG 4 cut(s) 410, 554, 701, 702
AfaI GTAC 7 cut(s) 110, 341, 356, 404, 488, 581, 1073
AfiI CCNNNNNNNGG 8 cut(s) 394, 437, 438, 508, 823, 901, 1081, 1082
AflII CTTAAG 1 cut(s) 44
AflIII ACRYGT 1 cut(s) 616
AgsI TTSAA 3 cut(s) 19, 751, 1003
AhdI GACNNNNNGTC 1 cut(s) 964
AjiI CACGTC 1 cut(s) 617
AjnI CCWGG 4 cut(s) 26, 640, 846, 868
AluBI AGCT 2 cut(s) 43, 230
AluI AGCT 2 cut(s) 43, 230
Alw26I GTCTC 4 cut(s) 92, 532, 617, 1052
AlwI GGATC 2 cut(s) 783, 937
AoxI GGCC 2 cut(s) 48, 662
ApeKI GCWGC 3 cut(s) 306, 830, 1184
AspLEI GCGC 1 cut(s) 1110
AspS9I GGNCC 4 cut(s) 468, 494, 662, 1153
AsuC2I CCSGG 2 cut(s) 135, 732
AsuHPI GGTGA 6 cut(s) 61, 461, 746, 950, 1036, 1183
AsuII TTCGAA 1 cut(s) 225
AvaII GGWCC 3 cut(s) 468, 494, 1153
BanI GGYRCC 1 cut(s) 1163
BanII GRGCYC 1 cut(s) 534
BarI GAAGNNNNNNTAC 2 cut(s) 386, 418
BauI CACGAG 1 cut(s) 707
BbsI GAAGAC 1 cut(s) 554
BbvI GCAGC 3 cut(s) 293, 817, 1171
BccI CCATC 2 cut(s) 313, 440
BceAI ACGGC 1 cut(s) 911
BciT130I CCWGG 4 cut(s) 28, 642, 848, 870
BcnI CCSGG 2 cut(s) 135, 732
BcoDI GTCTC 4 cut(s) 92, 532, 617, 1052
BfaI CTAG 1 cut(s) 284
BfrI CTTAAG 1 cut(s) 44
BfuAI ACCTGC 1 cut(s) 1007
BglI GCCNNNNNGGC 1 cut(s) 827
BisI GCNGC 3 cut(s) 307, 831, 1185
BlsI GCNGC 3 cut(s) 308, 832, 1186
BmcAI AGTACT 1 cut(s) 341
Bme1390I CCNGG 6 cut(s) 28, 135, 642, 732, 848, 870
Bme18I GGWCC 3 cut(s) 468, 494, 1153
BmeRI GACNNNNNGTC 1 cut(s) 964
BmgBI CACGTC 1 cut(s) 617
BmgT120I GGNCC 4 cut(s) 468, 494, 662, 1153
BmiI GGNNCC 4 cut(s) 495, 990, 1155, 1165
BmrFI CCNGG 6 cut(s) 28, 135, 642, 732, 848, 870
BmrI ACTGGG 1 cut(s) 890
BmsI GCATC 7 cut(s) 118, 313, 690, 712, 806, 1003, 1020
BmuI ACTGGG 1 cut(s) 890
BpiI GAAGAC 1 cut(s) 554
BplI GAGNNNNNCTC 2 cut(s) 1049, 1081
Bpu14I TTCGAA 1 cut(s) 225
BpuEI CTTGAG 1 cut(s) 527
BpuMI CCSGG 2 cut(s) 135, 732
BsaI GGTCTC 3 cut(s) 92, 532, 1052
BsaJI CCNNGG 9 cut(s) 27, 240, 730, 807, 822, 846, 868, 1047, 1167
BsaXI ACNNNNNCTCC 2 cut(s) 980, 1010
Bsc4I CCNNNNNNNGG 8 cut(s) 394, 437, 438, 508, 823, 901, 1081, 1082
Bse1I ACTGG 7 cut(s) 172, 220, 488, 608, 631, 673, 896
Bse3DI GCAATG 1 cut(s) 921
BseBI CCWGG 4 cut(s) 28, 642, 848, 870
BseDI CCNNGG 9 cut(s) 27, 240, 730, 807, 822, 846, 868, 1047, 1167
BseGI GGATG 5 cut(s) 328, 380, 580, 797, 817
BseLI CCNNNNNNNGG 8 cut(s) 394, 437, 438, 508, 823, 901, 1081, 1082
BseMI GCAATG 1 cut(s) 921
BseMII CTCAG 2 cut(s) 558, 1059
BseNI ACTGG 7 cut(s) 172, 220, 488, 608, 631, 673, 896
BseXI GCAGC 3 cut(s) 293, 817, 1171
BseYI CCCAGC 1 cut(s) 1157
BsgI GTGCAG 1 cut(s) 100
BshFI GGCC 2 cut(s) 50, 664
BshNI GGYRCC 1 cut(s) 1163
BsiSI CCGG 2 cut(s) 134, 732
BslFI GGGAC 3 cut(s) 150, 507, 627
BslI CCNNNNNNNGG 8 cut(s) 394, 437, 438, 508, 823, 901, 1081, 1082
BsmAI GTCTC 4 cut(s) 92, 532, 617, 1052
BsmFI GGGAC 3 cut(s) 150, 507, 627
BsnI GGCC 2 cut(s) 50, 664
Bso31I GGTCTC 3 cut(s) 92, 532, 1052
Bsp119I TTCGAA 1 cut(s) 225
Bsp1286I GDGCHC 1 cut(s) 534
Bsp1407I TGTACA 1 cut(s) 579
Bsp143I GATC 4 cut(s) 522, 775, 876, 942
Bsp19I CCATGG 1 cut(s) 1047
BspACI CCGC 2 cut(s) 931, 1161
BspANI GGCC 2 cut(s) 50, 664
BspCNI CTCAG 2 cut(s) 557, 1060
BspHI TCATGA 1 cut(s) 858
BspLI GGNNCC 4 cut(s) 495, 990, 1155, 1165
BspMI ACCTGC 1 cut(s) 1007
BspPI GGATC 2 cut(s) 783, 937
BspQI GCTCTTC 2 cut(s) 273, 720
BspT104I TTCGAA 1 cut(s) 225
BspT107I GGYRCC 1 cut(s) 1163
BspTI CTTAAG 1 cut(s) 44
BspTNI GGTCTC 3 cut(s) 92, 532, 1052
BsrDI GCAATG 1 cut(s) 921
BsrGI TGTACA 1 cut(s) 579
BsrI ACTGG 7 cut(s) 172, 220, 488, 608, 631, 673, 896
BssECI CCNNGG 9 cut(s) 27, 240, 730, 807, 822, 846, 868, 1047, 1167
BssMI GATC 4 cut(s) 522, 775, 876, 942
BssSI CACGAG 1 cut(s) 707
BssT1I CCWWGG 5 cut(s) 240, 807, 822, 1047, 1167
Bst2BI CACGAG 1 cut(s) 707
Bst2UI CCWGG 4 cut(s) 28, 642, 848, 870
Bst4CI ACNGT 4 cut(s) 121, 188, 604, 1117
Bst6I CTCTTC 3 cut(s) 273, 573, 720
BstAFI CTTAAG 1 cut(s) 44
BstAUI TGTACA 1 cut(s) 579
BstBI TTCGAA 1 cut(s) 225
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 4 cut(s) 336, 345, 544, 1068
BstDSI CCRYGG 1 cut(s) 1047
BstEII GGTNACC 1 cut(s) 1171
BstENI CCTNNNNNAGG 2 cut(s) 392, 506
BstF5I GGATG 5 cut(s) 328, 380, 580, 797, 817
BstHHI GCGC 1 cut(s) 1110
BstKTI GATC 4 cut(s) 525, 778, 879, 945
BstMAI GTCTC 4 cut(s) 92, 532, 617, 1052
BstMBI GATC 4 cut(s) 522, 775, 876, 942
BstMWI GCNNNNNNNGC 4 cut(s) 40, 188, 272, 827
BstNI CCWGG 4 cut(s) 28, 642, 848, 870
BstNSI RCATGY 2 cut(s) 266, 1108
BstPI GGTNACC 1 cut(s) 1171
BstSCI CCNGG 6 cut(s) 26, 133, 640, 730, 846, 868
BstV1I GCAGC 3 cut(s) 293, 817, 1171
BstV2I GAAGAC 1 cut(s) 554
BstX2I RGATCY 2 cut(s) 775, 942
BstXI CCANNNNNNTGG 2 cut(s) 1048, 1054
BstYI RGATCY 2 cut(s) 775, 942
BsuRI GGCC 2 cut(s) 50, 664
BtgI CCRYGG 1 cut(s) 1047
BtrI CACGTC 1 cut(s) 617
BtsCI GGATG 5 cut(s) 328, 380, 580, 797, 817
BtsIMutI CAGTG 3 cut(s) 213, 615, 678
BveI ACCTGC 1 cut(s) 1007
Cac8I GCNNGC 1 cut(s) 264
CciI TCATGA 1 cut(s) 858
CfoI GCGC 1 cut(s) 1110
Cfr13I GGNCC 4 cut(s) 468, 494, 662, 1153
Csp6I GTAC 7 cut(s) 109, 340, 355, 403, 487, 580, 1072
CspCI CAANNNNNGTGG 4 cut(s) 460, 495, 571, 606
CviAII CATG 6 cut(s) 160, 263, 457, 859, 1048, 1105
CviQI GTAC 7 cut(s) 109, 340, 355, 403, 487, 580, 1072
DdeI CTNAG 4 cut(s) 336, 345, 544, 1068
DpnI GATC 4 cut(s) 524, 777, 878, 944
DpnII GATC 4 cut(s) 522, 775, 876, 942
DriI GACNNNNNGTC 1 cut(s) 964
Eam1104I CTCTTC 3 cut(s) 273, 573, 720
Eam1105I GACNNNNNGTC 1 cut(s) 964
EarI CTCTTC 3 cut(s) 273, 573, 720
Eco130I CCWWGG 5 cut(s) 240, 807, 822, 1047, 1167
Eco24I GRGCYC 1 cut(s) 534
Eco31I GGTCTC 3 cut(s) 92, 532, 1052
Eco32I GATATC 1 cut(s) 79
Eco47I GGWCC 3 cut(s) 468, 494, 1153
Eco57I CTGAAG 4 cut(s) 410, 554, 701, 702
Eco91I GGTNACC 1 cut(s) 1171
EcoNI CCTNNNNNAGG 2 cut(s) 392, 506
EcoO109I RGGNCCY 1 cut(s) 1153
EcoO65I GGTNACC 1 cut(s) 1171
EcoRII CCWGG 4 cut(s) 26, 640, 846, 868
EcoRV GATATC 1 cut(s) 79
EcoT14I CCWWGG 5 cut(s) 240, 807, 822, 1047, 1167
EcoT22I ATGCAT 1 cut(s) 705
EcoT38I GRGCYC 1 cut(s) 534
ErhI CCWWGG 5 cut(s) 240, 807, 822, 1047, 1167
FaeI CATG 6 cut(s) 163, 266, 460, 862, 1051, 1108
FaqI GGGAC 3 cut(s) 150, 507, 627
FatI CATG 6 cut(s) 159, 262, 456, 858, 1047, 1104
Fnu4HI GCNGC 3 cut(s) 307, 831, 1185
FokI GGATG 5 cut(s) 335, 367, 587, 784, 824
FriOI GRGCYC 1 cut(s) 534
Fsp4HI GCNGC 3 cut(s) 307, 831, 1185
FspBI CTAG 1 cut(s) 284
GlaI GCGC 1 cut(s) 1109
GluI GCNGC 3 cut(s) 307, 831, 1185
GsaI CCCAGC 1 cut(s) 1161
HaeIII GGCC 2 cut(s) 50, 664
HapII CCGG 2 cut(s) 134, 732
HhaI GCGC 1 cut(s) 1110
Hin1II CATG 6 cut(s) 163, 266, 460, 862, 1051, 1108
Hin6I GCGC 1 cut(s) 1108
HinP1I GCGC 1 cut(s) 1108
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 1 cut(s) 206
HpaII CCGG 2 cut(s) 134, 732
HphI GGTGA 6 cut(s) 61, 461, 746, 950, 1036, 1183
Hpy166II GTNNAC 2 cut(s) 471, 580
Hpy188I TCNGA 4 cut(s) 547, 675, 876, 1069
Hpy8I GTNNAC 2 cut(s) 471, 580
Hpy99I CGWCG 1 cut(s) 797
HpyAV CCTTC 7 cut(s) 364, 388, 512, 766, 979, 1072, 1144
HpyCH4III ACNGT 4 cut(s) 121, 188, 604, 1117
HpyCH4IV ACGT 2 cut(s) 616, 784
HpyCH4V TGCA 3 cut(s) 117, 703, 1016
HpyF10VI GCNNNNNNNGC 4 cut(s) 40, 188, 272, 827
HpyF3I CTNAG 4 cut(s) 336, 345, 544, 1068
HpySE526I ACGT 2 cut(s) 616, 784
Hsp92II CATG 6 cut(s) 163, 266, 460, 862, 1051, 1108
HspAI GCGC 1 cut(s) 1108
Kzo9I GATC 4 cut(s) 522, 775, 876, 942
LguI GCTCTTC 2 cut(s) 273, 720
LmnI GCTCC 2 cut(s) 136, 988
Lsp1109I GCAGC 3 cut(s) 293, 817, 1171
LweI GCATC 7 cut(s) 118, 313, 690, 712, 806, 1003, 1020
MaeI CTAG 1 cut(s) 284
MaeII ACGT 2 cut(s) 616, 784
MaeIII GTNAC 7 cut(s) 311, 449, 652, 734, 881, 1100, 1171
MalI GATC 4 cut(s) 524, 777, 878, 944
MboI GATC 4 cut(s) 522, 775, 876, 942
MboII GAAGA 8 cut(s) 260, 403, 554, 560, 707, 1015, 1039, 1159
MflI RGATCY 2 cut(s) 775, 942
MhlI GDGCHC 1 cut(s) 534
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 825
Mph1103I ATGCAT 1 cut(s) 705
MseI TTAA 4 cut(s) 45, 153, 687, 756
MslI CAYNNNNRTG 2 cut(s) 926, 1052
MspA1I CMGCKG 1 cut(s) 1161
MspCI CTTAAG 1 cut(s) 44
MspI CCGG 2 cut(s) 134, 732
MspR9I CCNGG 6 cut(s) 28, 135, 642, 732, 848, 870
MvaI CCWGG 4 cut(s) 28, 642, 848, 870
MwoI GCNNNNNNNGC 4 cut(s) 40, 188, 272, 827
NciI CCSGG 2 cut(s) 135, 732
NcoI CCATGG 1 cut(s) 1047
NdeII GATC 4 cut(s) 522, 775, 876, 942
NlaIII CATG 6 cut(s) 163, 266, 460, 862, 1051, 1108
NlaIV GGNNCC 4 cut(s) 495, 990, 1155, 1165
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 5 cut(s) 449, 652, 734, 1100, 1171
NsiI ATGCAT 1 cut(s) 705
NspI RCATGY 2 cut(s) 266, 1108
NspV TTCGAA 1 cut(s) 225
PaeI GCATGC 1 cut(s) 266
PagI TCATGA 1 cut(s) 858
PciSI GCTCTTC 2 cut(s) 273, 720
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PflFI GACNNNGTC 2 cut(s) 740, 1177
PfoI TCCNGGA 1 cut(s) 133
PkrI GCNGC 3 cut(s) 308, 832, 1186
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
PpuMI RGGWCCY 1 cut(s) 1153
Psp5II RGGWCCY 1 cut(s) 1153
Psp6I CCWGG 4 cut(s) 26, 640, 846, 868
PspEI GGTNACC 1 cut(s) 1171
PspFI CCCAGC 1 cut(s) 1157
PspGI CCWGG 4 cut(s) 26, 640, 846, 868
PspN4I GGNNCC 4 cut(s) 495, 990, 1155, 1165
PspPI GGNCC 4 cut(s) 468, 494, 662, 1153
PspPPI RGGWCCY 1 cut(s) 1153
PsuI RGATCY 2 cut(s) 775, 942
PsyI GACNNNGTC 2 cut(s) 740, 1177
RsaI GTAC 7 cut(s) 110, 341, 356, 404, 488, 581, 1073
RsaNI GTAC 7 cut(s) 109, 340, 355, 403, 487, 580, 1072
RseI CAYNNNNRTG 2 cut(s) 926, 1052
SapI GCTCTTC 2 cut(s) 273, 720
SaqAI TTAA 4 cut(s) 45, 153, 687, 756
SatI GCNGC 3 cut(s) 307, 831, 1185
Sau3AI GATC 4 cut(s) 522, 775, 876, 942
Sau96I GGNCC 4 cut(s) 468, 494, 662, 1153
ScaI AGTACT 1 cut(s) 341
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 6 cut(s) 28, 135, 642, 732, 848, 870
SduI GDGCHC 1 cut(s) 534
SfaNI GCATC 7 cut(s) 118, 313, 690, 712, 806, 1003, 1020
SfuI TTCGAA 1 cut(s) 225
SinI GGWCC 3 cut(s) 468, 494, 1153
SmiMI CAYNNNNRTG 2 cut(s) 926, 1052
SmlI CTYRAG 2 cut(s) 44, 506
SmoI CTYRAG 2 cut(s) 44, 506
SphI GCATGC 1 cut(s) 266
SsiI CCGC 2 cut(s) 931, 1161
SspMI CTAG 1 cut(s) 284
StyD4I CCNGG 6 cut(s) 26, 133, 640, 730, 846, 868
StyI CCWWGG 5 cut(s) 240, 807, 822, 1047, 1167
TaaI ACNGT 4 cut(s) 121, 188, 604, 1117
TaiI ACGT 2 cut(s) 619, 787
TaqI TCGA 1 cut(s) 225
TatI WGTACW 2 cut(s) 339, 579
Tru1I TTAA 4 cut(s) 45, 153, 687, 756
Tru9I TTAA 4 cut(s) 45, 153, 687, 756
TscAI CASTG 3 cut(s) 220, 615, 685
TseFI GTSAC 5 cut(s) 449, 652, 734, 1100, 1171
TseI GCWGC 3 cut(s) 306, 830, 1184
Tsp45I GTSAC 5 cut(s) 449, 652, 734, 1100, 1171
TspDTI ATGAA 5 cut(s) 17, 139, 369, 375, 875
TspGWI ACGGA 4 cut(s) 261, 548, 900, 1023
TspRI CASTG 3 cut(s) 220, 615, 685
Tth111I GACNNNGTC 2 cut(s) 740, 1177
Vha464I CTTAAG 1 cut(s) 44
VpaK11BI GGWCC 3 cut(s) 468, 494, 1153
XagI CCTNNNNNAGG 2 cut(s) 392, 506
XceI RCATGY 2 cut(s) 266, 1108
XcmI CCANNNNNNNNNTGG 1 cut(s) 1045
XspI CTAG 1 cut(s) 284
ZrmI AGTACT 1 cut(s) 341
Zsp2I ATGCAT 1 cut(s) 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.